Pub Date : 2023-06-01DOI: 10.1177/14690667231183046
Metin Demirel, Sahabettin Selek
Vitamin K is an essential lipophilic vitamin that acts as a coenzyme in several metabolic pathways. Accurate measurement of apolar metabolites transported by lipoproteins in serum matrices requires high-recovery extractions of vitamin K and its derivatives following standardized protocols. Conventionally developed methods in this field have predominantly employed solid-phase extraction for the measurement of vitamin K and its derivatives. In this study, our objective was to develop an enzyme-assisted extraction method for the precise measurement of vitamin K and its derivatives. Our methodology involved mixing 450 µL of serum samples with 50 µL of an internal standard and 50 µL of a lipase enzyme solution. Following vortexing, the mixture was incubated at 37°C for 15 min to activate the enzymes. The enzyme reaction was subsequently quenched with a mixture of 250 µL of methanol and 1 mL of hexane, followed by centrifugation at 12,000 g for 5 min. The upper phase was collected, concentrated using a concentrator device, and dissolved in a 100 µL solution of methanol/acetone/isopropanol (7:1:1, v/v/v) for analysis. Spectrum analysis was performed using the open-source MZmine 3 software, and a reference interval was established using the Python programming language on the Google Colab platform. The developed method for measuring vitamin K and its derivatives exhibited limit of detection and limit of quantitation values of 0.005 and 0.01 ng/mL, respectively. In conclusion, our study presents a precise and reliable method for the measurement of vitamin K and its derivatives using enzyme-assisted extraction.
{"title":"Measurement of vitamin K and its derivatives in human plasma through enzyme-assisted liquid chromatography-tandem mass spectrometry assay.","authors":"Metin Demirel, Sahabettin Selek","doi":"10.1177/14690667231183046","DOIUrl":"https://doi.org/10.1177/14690667231183046","url":null,"abstract":"<p><p>Vitamin K is an essential lipophilic vitamin that acts as a coenzyme in several metabolic pathways. Accurate measurement of apolar metabolites transported by lipoproteins in serum matrices requires high-recovery extractions of vitamin K and its derivatives following standardized protocols. Conventionally developed methods in this field have predominantly employed solid-phase extraction for the measurement of vitamin K and its derivatives. In this study, our objective was to develop an enzyme-assisted extraction method for the precise measurement of vitamin K and its derivatives. Our methodology involved mixing 450 µL of serum samples with 50 µL of an internal standard and 50 µL of a lipase enzyme solution. Following vortexing, the mixture was incubated at 37°C for 15 min to activate the enzymes. The enzyme reaction was subsequently quenched with a mixture of 250 µL of methanol and 1 mL of hexane, followed by centrifugation at 12,000 <i>g</i> for 5 min. The upper phase was collected, concentrated using a concentrator device, and dissolved in a 100 µL solution of methanol/acetone/isopropanol (7:1:1, v/v/v) for analysis. Spectrum analysis was performed using the open-source MZmine 3 software, and a reference interval was established using the Python programming language on the Google Colab platform. The developed method for measuring vitamin K and its derivatives exhibited limit of detection and limit of quantitation values of 0.005 and 0.01 ng/mL, respectively. In conclusion, our study presents a precise and reliable method for the measurement of vitamin K and its derivatives using enzyme-assisted extraction.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study is to gain insights into the underlying metabolic transformations that occurred during the whole progression of cecal ligation and puncture (CLP)-induced sepsis, thus providing new targets for its treatment. High-performance liquid chromatography of quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS) combined with multivariate statistical techniques was used to detect the s in serum from septic mice. Fifty male mice were divided into two groups, including the sham group (n = 7) and the CLP-induced sepsis group (n = 43). Animals were sacrificed at 1, 3, 5, and 7 days post-CLP and then serum were collected for metabolomic analysis. Multivariate regression analysis was carried out through MetaboAnalyst 5.0, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), to identify the s and screen out the related differential metabolites. Besides, the KEGG pathway analysis was used to analyze the related metabolic pathways in which the identified metabolites were involved. Based on the fold change (FC > 2.0 or <0.5), variable important in projection (VIP > 1.2), and P value (P < 0.05), we found 26, 17, 21, and 17 metabolites in septic mice at 1, 3, 5, and 7 days post-CLP, respectively, compared with that of the sham group. The PCA and PLS-DA pattern recognition showed a cluster-type distribution between the sham group and the CLP group. Dysregulated amino acid metabolism, as well as disturbed nucleotide metabolism, is observed. Several important metabolic pathways were identified between the sham group and the CLP group. Among them, phenylalanine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis showed striking at day 1 post-CLP. At day 3, phenylalanine, tyrosine, and tryptophan biosynthesis changed significantly. However, as the disease process, only pyrimidine metabolism showed the most significant alternation, compared to the sham group. Several differential metabolites were identified in the CLP group compared with that of the sham group and they were presented with dynamic alternation at different time points post-CLP, indicating metabolic disturbance occurred throughout the whole sepsis progression.
{"title":"Dynamic changes of serum metabolite profiling in septic mice based on high performance liquid chromatography of quadrupole time of flight mass spectrometry analysis.","authors":"Shutong Li, Qi Zeng, Shentang Li, Yarong Liu, Yang Feng, Fang Chen, Lianhong Zou, Xiehong Liu, Yanjuan Liu, Yu Jiang","doi":"10.1177/14690667231179565","DOIUrl":"https://doi.org/10.1177/14690667231179565","url":null,"abstract":"<p><p>The objective of this study is to gain insights into the underlying metabolic transformations that occurred during the whole progression of cecal ligation and puncture (CLP)-induced sepsis, thus providing new targets for its treatment. High-performance liquid chromatography of quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS) combined with multivariate statistical techniques was used to detect the s in serum from septic mice. Fifty male mice were divided into two groups, including the sham group (<i>n</i> = 7) and the CLP-induced sepsis group (<i>n</i> = 43). Animals were sacrificed at 1, 3, 5, and 7 days post-CLP and then serum were collected for metabolomic analysis. Multivariate regression analysis was carried out through MetaboAnalyst 5.0, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), to identify the s and screen out the related differential metabolites. Besides, the KEGG pathway analysis was used to analyze the related metabolic pathways in which the identified metabolites were involved. Based on the fold change (FC > 2.0 or <0.5), variable important in projection (VIP > 1.2), and <i>P</i> value (<i>P</i> < 0.05), we found 26, 17, 21, and 17 metabolites in septic mice at 1, 3, 5, and 7 days post-CLP, respectively, compared with that of the sham group. The PCA and PLS-DA pattern recognition showed a cluster-type distribution between the sham group and the CLP group. Dysregulated amino acid metabolism, as well as disturbed nucleotide metabolism, is observed. Several important metabolic pathways were identified between the sham group and the CLP group. Among them, phenylalanine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis showed striking at day 1 post-CLP. At day 3, phenylalanine, tyrosine, and tryptophan biosynthesis changed significantly. However, as the disease process, only pyrimidine metabolism showed the most significant alternation, compared to the sham group. Several differential metabolites were identified in the CLP group compared with that of the sham group and they were presented with dynamic alternation at different time points post-CLP, indicating metabolic disturbance occurred throughout the whole sepsis progression.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10067019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1177/14690667231174446
Kalpana Talari, Sai Krishna Ganji, Raja Rajeswari Tiruveedula
Bisphenols are known endocrine disruptors commonly utilized in food packaging and storage materials, which frequently come into touch with multiple food products packed in them. The bisphenols in fish feed and other feed materials for aquatic organisms are harmful. The consumption of such marine foods is hazardous. Hence, the feed of aquatic products needs to be verified for the presence of bisphenols. The present study was focused on developing and validating a rapid, selective, and sensitive method to quantify 11 bisphenols from the fish feed with dispersive solid-phase extraction, which was cleaned by an optimized amount of activated carbon spheres and silylated by N,O-bis(trimethylsilyl)trifluoro acetamide and analyzed by gas chromatography-mass spectrometry. The new method was rigorously tested and verified after carefully tuning various parameters affecting analyte recovery. Limit of detection (LOD) were set at 0.5-5 ng/g and limit of quantification (LOQ) at 1-10 ng/g, respectively, resulting in 95-114% recoveries. Interday and intraday precisions in terms of relative standard deviation were found to be less than 11%. The proposed approach was effectively applied in floating and sinking fish feeds. The obtained results showed that higher concentration of bisphenol A, followed by bisphenol TMC, and bisphenol M at a concentration of 256.10, 159.01, and 168.82 ng/g in floating feed and 88.04, 200.79, and 98.03 ng/g in sinking feed samples, respectively.
{"title":"Gas chromatography-mass spectrometric determination of bisphenol residues by dispersive solid phase extraction followed by activated carbon spheres cleanup from fish feed samples.","authors":"Kalpana Talari, Sai Krishna Ganji, Raja Rajeswari Tiruveedula","doi":"10.1177/14690667231174446","DOIUrl":"https://doi.org/10.1177/14690667231174446","url":null,"abstract":"<p><p>Bisphenols are known endocrine disruptors commonly utilized in food packaging and storage materials, which frequently come into touch with multiple food products packed in them. The bisphenols in fish feed and other feed materials for aquatic organisms are harmful. The consumption of such marine foods is hazardous. Hence, the feed of aquatic products needs to be verified for the presence of bisphenols. The present study was focused on developing and validating a rapid, selective, and sensitive method to quantify 11 bisphenols from the fish feed with dispersive solid-phase extraction, which was cleaned by an optimized amount of activated carbon spheres and silylated by N,O-bis(trimethylsilyl)trifluoro acetamide and analyzed by gas chromatography-mass spectrometry. The new method was rigorously tested and verified after carefully tuning various parameters affecting analyte recovery. Limit of detection (LOD) were set at 0.5-5 ng/g and limit of quantification (LOQ) at 1-10 ng/g, respectively, resulting in 95-114% recoveries. Interday and intraday precisions in terms of relative standard deviation were found to be less than 11%. The proposed approach was effectively applied in floating and sinking fish feeds. The obtained results showed that higher concentration of bisphenol A, followed by bisphenol TMC, and bisphenol M at a concentration of 256.10, 159.01, and 168.82 ng/g in floating feed and 88.04, 200.79, and 98.03 ng/g in sinking feed samples, respectively.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1177/14690667231181340
Chandramohan Alluri, G Veera Raghava Sharma
Edoxaban is an anti-coagulant medication and a director factor Xa inhibitor. A novel reverse phase liquid chromatography-mass spectrometry compatible method developed for separation and identification of new oxidative degradation impurities in edoxaban tosylate hydrate drug substance. The separation of three oxidative degradation impurities was achieved by using YMC Triart phenyl (250 × 4.6) mm, 5µm column with mobile phase containing gradient elution of mobile phase-A as 10mM ammonium acetate and mobile phase-B as acetonitrile:methanol (1:1)% (v/v). The flow rate of the mobile phase is 0.7mL/min with a column temperature of 40 °C and detection wavelength of 290nm. Edoxaban tosylate hydrate shows significant degradation in oxidative stress conditions and forms three oxidative degradation products. The degradation products were identified and characterized by using a high-resolution mass spectrometry quadrupole-time of flight mass detector. The three oxidative degradation impurities of Edoxaban drug substance were well resolved with each other and along with Edoxaban drug substance peak. Among the three oxidative degradation impurities di-N-oxide impurity was the new oxidative degradation impurity identified for the first time and a novel reverse-phase high-performance liquid chromatography method was developed for separation of the three oxidative degradation impurities.
依多沙班是一种抗凝血药物,也是一种指导因子Xa抑制剂。建立了一种新的反相液相色谱-质谱兼容方法,用于分离鉴定水合戊沙班酯原料药中新的氧化降解杂质。采用YMC Triart苯基(250 × 4.6) mm, 5µm色谱柱,流动相梯度洗脱,流动相a为10 mm乙酸铵,流动相b为乙腈:甲醇(1:1)% (v/v),实现了3种氧化降解杂质的分离。流动相流速为0.7 mL/min,柱温为40℃,检测波长为290 nm。水合戊磺酸乙多沙班在氧化应激条件下表现出明显的降解,形成三种氧化降解产物。利用高分辨率质谱四极杆飞行时间质量检测器对降解产物进行了鉴定和表征。依多沙班原料药的三种氧化降解杂质相互降解良好,并随依多沙班原料药峰出现。在三种氧化降解杂质中,二氮氧化物杂质是首次发现的新的氧化降解杂质,并建立了一种新的反相高效液相色谱法分离这三种氧化降解杂质。
{"title":"Liquid chromatography separation and identification of new oxidative degradation impurity in edoxaban tosylate hydrate drug substance by using liquid chromatography with tandem mass spectrometry.","authors":"Chandramohan Alluri, G Veera Raghava Sharma","doi":"10.1177/14690667231181340","DOIUrl":"https://doi.org/10.1177/14690667231181340","url":null,"abstract":"<p><p>Edoxaban is an anti-coagulant medication and a director factor Xa inhibitor. A novel reverse phase liquid chromatography-mass spectrometry compatible method developed for separation and identification of new oxidative degradation impurities in edoxaban tosylate hydrate drug substance. The separation of three oxidative degradation impurities was achieved by using YMC Triart phenyl (250 × 4.6) mm, 5<b> </b>µm column with mobile phase containing gradient elution of mobile phase-A as 10<b> </b>mM ammonium acetate and mobile phase-B as acetonitrile:methanol (1:1)% (v/v). The flow rate of the mobile phase is 0.7<b> </b>mL/min with a column temperature of 40 °C and detection wavelength of 290<b> </b>nm. Edoxaban tosylate hydrate shows significant degradation in oxidative stress conditions and forms three oxidative degradation products. The degradation products were identified and characterized by using a high-resolution mass spectrometry quadrupole-time of flight mass detector. The three oxidative degradation impurities of Edoxaban drug substance were well resolved with each other and along with Edoxaban drug substance peak. Among the three oxidative degradation impurities di-N-oxide impurity was the new oxidative degradation impurity identified for the first time and a novel reverse-phase high-performance liquid chromatography method was developed for separation of the three oxidative degradation impurities.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conventional endoscopic biopsy tests are not suitable for early detection of the acute onset and progression of peptic ulcer as well as various gastric complications. This also limits its suitability for widespread population-based screening and consequently, many people with complex gastric phenotypes remain undiagnosed. Here, we demonstrate a new non-invasive methodology for accurate diagnosis and classification of various gastric disorders exploiting a pattern-recognition-based cluster analysis of a breathomics dataset generated from a simple residual gas analyzer-mass spectrometry. The clustering approach recognizes unique breathograms and "breathprints" signatures that clearly reflect the specific gastric condition of an individual person. The method can selectively distinguish the breath of peptic ulcer and other gastric dysfunctions like dyspepsia, gastritis, and gastroesophageal reflux disease patients from the exhaled breath of healthy individuals with high diagnostic sensitivity and specificity. Moreover, the clustering method exhibited a reasonable power to selectively classify the early-stage and high-risk gastric conditions with/without ulceration, thus opening a new non-invasive analytical avenue for early detection, follow-up, and fast population-based robust screening strategy of gastric complications in the real-world clinical domain.
{"title":"A pattern-recognition-based clustering method for non-invasive diagnosis and classification of various gastric conditions.","authors":"Abhijit Maity, Sayoni Bhattacharya, Anil C Mahato, Sujit Chaudhuri, Manik Pradhan","doi":"10.1177/14690667231174350","DOIUrl":"https://doi.org/10.1177/14690667231174350","url":null,"abstract":"<p><p>Conventional endoscopic biopsy tests are not suitable for early detection of the acute onset and progression of peptic ulcer as well as various gastric complications. This also limits its suitability for widespread population-based screening and consequently, many people with complex gastric phenotypes remain undiagnosed. Here, we demonstrate a new non-invasive methodology for accurate diagnosis and classification of various gastric disorders exploiting a pattern-recognition-based cluster analysis of a breathomics dataset generated from a simple residual gas analyzer-mass spectrometry. The clustering approach recognizes unique breathograms and \"breathprints\" signatures that clearly reflect the specific gastric condition of an individual person. The method can selectively distinguish the breath of peptic ulcer and other gastric dysfunctions like dyspepsia, gastritis, and gastroesophageal reflux disease patients from the exhaled breath of healthy individuals with high diagnostic sensitivity and specificity. Moreover, the clustering method exhibited a reasonable power to selectively classify the early-stage and high-risk gastric conditions with/without ulceration, thus opening a new non-invasive analytical avenue for early detection, follow-up, and fast population-based robust screening strategy of gastric complications in the real-world clinical domain.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9689204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1177/14690667221142699
Kuanliang Shao, Ge Sun, Mariah Gomez, Xinghua Liu, Jingsong Zhang
Thermal decomposition of cycloheptane was studied using flash pyrolysis coupled with vacuum ultraviolet (118.2 nm) single photon ionization time-of-flight mass spectrometry at temperatures ranging from 295 K to 1380 K. C-C bond breaking of cycloheptane leading to the 1,7-heptyl diradical was considered as the initiation step. The 1,7-heptyl diradical could readily isomerize to 1-heptene and decompose into several fragments, with dissociation to •C4H9 and •C3H5 as the predominant product channel. The 1,7-heptyl diradical could undergo direct dissociation, as evidenced by the production of the C5H10 species. Quantum chemistry calculations at UCCSD(T)/cc-pVDZ//UB3LYP/cc-pVDZ level of theory on the initial reaction pathways of cycloheptane were also carried out to support the experimental observations. Other possible initiation channels, as well as some secondary reaction products, were also identified.
{"title":"Flash pyrolysis vacuum ultraviolet photoionization mass spectrometry of cycloheptane: A study of the initial decomposition mechanism.","authors":"Kuanliang Shao, Ge Sun, Mariah Gomez, Xinghua Liu, Jingsong Zhang","doi":"10.1177/14690667221142699","DOIUrl":"https://doi.org/10.1177/14690667221142699","url":null,"abstract":"<p><p>Thermal decomposition of cycloheptane was studied using flash pyrolysis coupled with vacuum ultraviolet (118.2 nm) single photon ionization time-of-flight mass spectrometry at temperatures ranging from 295 K to 1380 K. C-C bond breaking of cycloheptane leading to the 1,7-heptyl diradical was considered as the initiation step. The 1,7-heptyl diradical could readily isomerize to 1-heptene and decompose into several fragments, with dissociation to •C<sub>4</sub>H<sub>9</sub> and •C<sub>3</sub>H<sub>5</sub> as the predominant product channel. The 1,7-heptyl diradical could undergo direct dissociation, as evidenced by the production of the C<sub>5</sub>H<sub>10</sub> species. Quantum chemistry calculations at UCCSD(T)/cc-pVDZ//UB3LYP/cc-pVDZ level of theory on the initial reaction pathways of cycloheptane were also carried out to support the experimental observations. Other possible initiation channels, as well as some secondary reaction products, were also identified.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9600274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1177/14690667231153777
Adam R Michalik, Nathan W Fenwick, Richard Telford, Archie W Johnson, William Hc Martin, Richard D Bowen
The electron ionisation mass spectra of an extensive set of 53 ionised monosubstituted and disubstituted cinnamamides [XC6H4CH=CHCONH2, X = H, F, Cl, Br, I, CH3, CH3O, CF3, NO2, CH3CH2, (CH3)2CH and (CH3)3C; and XYC6H3CH=CHCONH2, X = Y = Cl; and X, Y = F, Cl or Br] are reported and discussed. Particular attention is paid to the significance of loss of the substituent, X, from the 2-position, via a rearrangement that is sometimes known as a proximity effect, which has been reported for a range of radical-cations, but is shown in this work to be especially important for ionised cinnamamides. When X is in the 2-position of the aromatic ring, [M - X]+ is formed to a far greater extent than [M - H]+; in contrast, when X is in the 3-position or 4-position, [M - H]+ is generally much more important than [M - X]+. Parallel trends are found in the spectra of XYC6H3CH=CHCONH2: the signal for [M - X]+ dominates that for [M - Y]+ when X is in the 2-position and Y in the 4-position or 5-position, irrespective of the nature of X and Y. Further insight is obtained by studying the competition between expulsion of X· and alternative fragmentations that may be described as simple cleavages. Loss of ·NH2 results in the formation of a substituted cinnamoyl cation, [XC6H4CH=CHCO]+ or [XYC6H3CH=CHCO]+; this process competes far less effectively with the proximity effect when X is in the 2-position than when it is in the 3-position or 4-position. Additional information has been obtained by investigating the competition between formation of [M - H]+ by the proximity effect and loss of CH3· by cleavage of a 4-alkyl group to give a benzylic cation, [R1R2CC6H4CH=CHCONH2]+ (R1, R2 = H, CH3).
53种单取代和双取代肉桂酰胺的电子电离质谱[XC6H4CH=CHCONH2, X = H, F, Cl, Br, I, CH3, ch30, CF3, NO2, CH3CH2, (CH3)2CH和(CH3)3C]和XYC6H3CH=CHCONH2, X = Y = Cl;和X, Y = F, Cl或Br]的报道和讨论。特别注意的是取代基,X,从2位损失的重要性,通过重排,有时被称为邻近效应,这已经报道了一系列的自由基阳离子,但在这项工作中显示,对电离肉桂酰胺特别重要。当X位于芳环的2位时,[M - X]+的形成程度远远大于[M - H]+;相反,当X处于3位或4位时,[M - H]+通常比[M - X]+重要得多。在XYC6H3CH=CHCONH2的光谱中发现了类似的趋势:当X处于2位,Y处于4位或5位时,无论X和Y的性质如何,[M - X]+的信号都优于[M - Y]+的信号。通过研究X·的排出和可被描述为简单解理的选择性片段之间的竞争,我们得到了进一步的认识。·NH2的损失导致形成取代的肉桂基阳离子,[XC6H4CH=CHCO]+或[XYC6H3CH=CHCO]+;当X位于2位时,这个过程与邻近效应的竞争远不如位于3位或4位时有效。通过研究邻近效应形成的[M - H]+和4-烷基裂解生成的苯基阳离子[R1R2CC6H4CH=CHCONH2]+ (R1, R2 = H, CH3)损失的CH3·之间的竞争,获得了更多的信息。
{"title":"Proximity effects in the electron ionisation mass spectra of substituted cinnamamides.","authors":"Adam R Michalik, Nathan W Fenwick, Richard Telford, Archie W Johnson, William Hc Martin, Richard D Bowen","doi":"10.1177/14690667231153777","DOIUrl":"https://doi.org/10.1177/14690667231153777","url":null,"abstract":"<p><p>The electron ionisation mass spectra of an extensive set of 53 ionised monosubstituted and disubstituted cinnamamides [XC<sub>6</sub>H<sub>4</sub>CH=CHCONH<sub>2</sub>, X = H, F, Cl, Br, I, CH<sub>3</sub>, CH<sub>3</sub>O, CF<sub>3</sub>, NO<sub>2</sub>, CH<sub>3</sub>CH<sub>2</sub>, (CH<sub>3</sub>)<sub>2</sub>CH and (CH<sub>3</sub>)<sub>3</sub>C; and XYC<sub>6</sub>H<sub>3</sub>CH=CHCONH<sub>2</sub>, X = Y = Cl; and X, Y = F, Cl or Br] are reported and discussed. Particular attention is paid to the significance of loss of the substituent, X, from the 2-position, via a rearrangement that is sometimes known as a proximity effect, which has been reported for a range of radical-cations, but is shown in this work to be especially important for ionised cinnamamides. When X is in the 2-position of the aromatic ring, [M - X]<sup>+</sup> is formed to a far greater extent than [M - H]<sup>+</sup>; in contrast, when X is in the 3-position or 4-position, [M - H]<sup>+</sup> is generally much more important than [M - X]<sup>+</sup>. Parallel trends are found in the spectra of XYC<sub>6</sub>H<sub>3</sub>CH=CHCONH<sub>2</sub>: the signal for [M - X]<sup>+</sup> dominates that for [M - Y]<sup>+</sup> when X is in the 2-position and Y in the 4-position or 5-position, irrespective of the nature of X and Y. Further insight is obtained by studying the competition between expulsion of X<sup>·</sup> and alternative fragmentations that may be described as simple cleavages. Loss of <sup>·</sup>NH<sub>2</sub> results in the formation of a substituted cinnamoyl cation, [XC<sub>6</sub>H<sub>4</sub>CH=CHCO]<sup>+</sup> or [XYC<sub>6</sub>H<sub>3</sub>CH=CHCO]<sup>+</sup>; this process competes far less effectively with the proximity effect when X is in the 2-position than when it is in the 3-position or 4-position. Additional information has been obtained by investigating the competition between formation of [M - H]<sup>+</sup> by the proximity effect and loss of CH<sub>3</sub><sup>·</sup> by cleavage of a 4-alkyl group to give a benzylic cation, [R<sup>1</sup>R<sup>2</sup>CC<sub>6</sub>H<sub>4</sub>CH=CHCONH<sub>2</sub>]<sup>+</sup> (R<sup>1</sup>, R<sup>2</sup> = H, CH<sub>3</sub>).</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9246388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1177/14690667221139419
Julia Taubert, Matthias Vogt, Robert Langer
A comparative mass spectrometric investigation using electrospray ionisation (ESI) and liquid injection field desorption/ionisation (LIFDI) techniques is reported for the highly luminescent and cationic copper cluster [(PCP)Cu] (1[Formula: see text], PCP = [1,3-(PhP)CH]). Depending on the available counter ion X, ion pairs consisting of the original or a modified cluster cation and the weakly coordinating counter ion can be detected by LIFDI-high-resolution-mass spectrometry in addition to the cluster cation. Notably, only large counter ions with an extremely low tendency for metal coordination give rise to the observation of ion pairs, whereas smaller ions such as BF do not show peaks corresponding to ion pairs in their mass spectra. In principle, two pathways were identified for the formation of positively charged ion pairs: (i) association of a generated Cu ion to the neutral ion pair [(PCP)Cu]X (1+X, X = BAr, BAr) and (ii) abstraction of an electron from the neutral ion pair [(PCP)Cu]X (1+X), leading to the oxidised ion pair [1+X][Formula: see text] (X = Al(OR)).
{"title":"Mass spectrometric detection of ion pairs containing rigid copper clusters and weakly coordinating counter ions using liquid injection field desorption/ionisation.","authors":"Julia Taubert, Matthias Vogt, Robert Langer","doi":"10.1177/14690667221139419","DOIUrl":"https://doi.org/10.1177/14690667221139419","url":null,"abstract":"<p><p>A comparative mass spectrometric investigation using electrospray ionisation (ESI) and liquid injection field desorption/ionisation (LIFDI) techniques is reported for the highly luminescent and cationic copper cluster [(PCP)<math><msub><mrow></mrow><mn>3</mn></msub></math>Cu<math><msub><mrow></mrow><mn>4</mn></msub></math>]<math><msup><mrow></mrow><mo>+</mo></msup></math> (<b>1[Formula: see text]</b>, PCP = [1,3-(Ph<math><msub><mrow></mrow><mn>2</mn></msub></math>P)<math><msub><mrow></mrow><mn>2</mn></msub></math>C<math><msub><mrow></mrow><mn>6</mn></msub></math>H<math><msub><mrow></mrow><mn>3</mn></msub></math>]<math><msup><mrow></mrow><mo>-</mo></msup></math>). Depending on the available counter ion X<math><msup><mrow></mrow><mo>-</mo></msup></math>, ion pairs consisting of the original or a modified cluster cation and the weakly coordinating counter ion can be detected by LIFDI-high-resolution-mass spectrometry in addition to the cluster cation. Notably, only large counter ions with an extremely low tendency for metal coordination give rise to the observation of ion pairs, whereas smaller ions such as BF<math><msubsup><mrow></mrow><mn>4</mn><mo>-</mo></msubsup></math> do not show peaks corresponding to ion pairs in their mass spectra. In principle, two pathways were identified for the formation of positively charged ion pairs: (i) association of a generated Cu<math><msup><mrow></mrow><mo>+</mo></msup></math> ion to the neutral ion pair [(PCP)<math><msub><mrow></mrow><mn>3</mn></msub></math>Cu<math><msub><mrow></mrow><mn>4</mn></msub></math>]X (<b>1+X</b>, X<math><msup><mrow></mrow><mo>-</mo></msup></math> = BAr<math><msubsup><mrow></mrow><mn>20</mn><mi>F</mi></msubsup></math>, BAr<math><msubsup><mrow></mrow><mn>24</mn><mi>F</mi></msubsup></math>) and (ii) abstraction of an electron from the neutral ion pair [(PCP)<math><msub><mrow></mrow><mn>3</mn></msub></math>Cu<math><msub><mrow></mrow><mn>4</mn></msub></math>]X (<b>1+X</b>), leading to the oxidised ion pair <b>[1+X][Formula: see text]</b> (X<math><msup><mrow></mrow><mo>-</mo></msup></math> = Al(OR<math><msup><mrow></mrow><mi>F</mi></msup></math>)<math><msub><mrow></mrow><mn>4</mn></msub></math>).</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9339495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1177/14690667231162345
Vivek Dhiman, Balasaheb B Chavan, Niharika Ramarapu, Gananadhamu Samanthula
Osimertinib mesylate is a third-generation epidermal growth factor receptor tyrosine kinase inhibitor used to treat nonsmall-cell lung cancer. The objective was to understand in silico prediction and chemical-based stress testing of the osimertinib mesylate. A total of eight degradation products (DPs) were formed under chemical stress testing. An in silico tool viz., Zeneth predicted a higher percentage of DPs. The separation of all the DPs was achieved using reversed phase high-performance liquid chromatography, employing X-Bridge C18 column with ammonium acetate (pH adjusted to 7.50 with ammonia) and acetonitrile as mobile phase. The overall results showed it underwent significant degradation in acidic, alkaline, and oxidative conditions. In rest of the conditions, osimertinib mesylate was found to be stable or slight degradation was observed in photolytic condition. The structure of DPs was elucidated with a comparison of data generated from high-resolution mass spectrometry (HRMS) of osimertinib mesylate and its degradation products. To confirm the unambiguous regioisomers, one-dimensional (1D) and two-dimentional (2D) nuclear magnetic resonance studies were performed. Furthermore, the N-oxide position was assigned for the first time using the Meisenheimer rearrangement reaction in atmospheric pressure chemical ionization mode. Interestingly, an unusual reaction of DP2 formation was observed at alkaline conditions. In silico tools such as DEREK and Sarah predicted osimertinib mesylate and most of the DPs found to be structural alert for mutagenicity.
{"title":"Insight into <i>in silico</i> prediction and chemical degradation study of osimertinib mesylate by LC-HRMS and NMR: Investigation of a typical case of alkaline pH-mediated oxidative degradation product.","authors":"Vivek Dhiman, Balasaheb B Chavan, Niharika Ramarapu, Gananadhamu Samanthula","doi":"10.1177/14690667231162345","DOIUrl":"https://doi.org/10.1177/14690667231162345","url":null,"abstract":"Osimertinib mesylate is a third-generation epidermal growth factor receptor tyrosine kinase inhibitor used to treat nonsmall-cell lung cancer. The objective was to understand in silico prediction and chemical-based stress testing of the osimertinib mesylate. A total of eight degradation products (DPs) were formed under chemical stress testing. An in silico tool viz., Zeneth predicted a higher percentage of DPs. The separation of all the DPs was achieved using reversed phase high-performance liquid chromatography, employing X-Bridge C18 column with ammonium acetate (pH adjusted to 7.50 with ammonia) and acetonitrile as mobile phase. The overall results showed it underwent significant degradation in acidic, alkaline, and oxidative conditions. In rest of the conditions, osimertinib mesylate was found to be stable or slight degradation was observed in photolytic condition. The structure of DPs was elucidated with a comparison of data generated from high-resolution mass spectrometry (HRMS) of osimertinib mesylate and its degradation products. To confirm the unambiguous regioisomers, one-dimensional (1D) and two-dimentional (2D) nuclear magnetic resonance studies were performed. Furthermore, the N-oxide position was assigned for the first time using the Meisenheimer rearrangement reaction in atmospheric pressure chemical ionization mode. Interestingly, an unusual reaction of DP2 formation was observed at alkaline conditions. In silico tools such as DEREK and Sarah predicted osimertinib mesylate and most of the DPs found to be structural alert for mutagenicity.","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9238890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1177/14690667231164766
Jiří Novák, Kevin A Schug, Vladimír Havlíček
Gaussian and exponentially modified Gaussian functions were incorporated into integrating algorithms used by an open-source, cross-platform tool called CycloBranch. The quantitation is demonstrated on bacterial pyoverdines separated by fine isotope features. Using our algorithm, we can separate the m/z values 694.25802 and 694.26731 (a 0.009 Da difference), where the former belongs to the most intense peak of pyoverdine D (PvdD), and the latter to the second most intense peak of pyoverdine E (PvdE) in the respective isotopic clusters of [M + Fe-H]2+ ions. The areas under chromatographic curves of standards were analyzed for the limit of detection (LOD), limit of quantitation (LOQ), and regression coefficient calculations. The quantitative module returned a LOD and LOQ of 1.4 and 4.3 ng/mL, respectively, for both PvdD and PvdE in human urine. If present and detected in mass spectra, the intensities of user-defined [M + H]+, [M + Na]+, [M + K]+, [M + Fe-H]2+, or other ion types, can be accumulated and used for quantitation. The quantitation result is returned by CycloBranch in seconds or minutes, contrary to an hours-long manual approach, prone to user-born errors originating from necessary copying among various software environments. Native Bruker, Waters, Thermo, txt, mgf, mzML, and mzXML data formats are supported in CycloBranch, which is freely available at https://ms.biomed.cas.cz/cyclobranch.
{"title":"Quantitation of small molecules from liquid chromatography-mass spectrometric accurate mass datasets using CycloBranch.","authors":"Jiří Novák, Kevin A Schug, Vladimír Havlíček","doi":"10.1177/14690667231164766","DOIUrl":"https://doi.org/10.1177/14690667231164766","url":null,"abstract":"<p><p>Gaussian and exponentially modified Gaussian functions were incorporated into integrating algorithms used by an open-source, cross-platform tool called CycloBranch. The quantitation is demonstrated on bacterial pyoverdines separated by fine isotope features. Using our algorithm, we can separate the m/z values 694.25802 and 694.26731 (a 0.009 Da difference), where the former belongs to the most intense peak of pyoverdine D (PvdD), and the latter to the second most intense peak of pyoverdine E (PvdE) in the respective isotopic clusters of [M + Fe-H]<sup>2+</sup> ions. The areas under chromatographic curves of standards were analyzed for the limit of detection (LOD), limit of quantitation (LOQ), and regression coefficient calculations. The quantitative module returned a LOD and LOQ of 1.4 and 4.3 ng/mL, respectively, for both PvdD and PvdE in human urine. If present and detected in mass spectra, the intensities of user-defined [M + H]<sup>+</sup>, [M + Na]<sup>+</sup>, [M + K]<sup>+</sup>, [M + Fe-H]<sup>2+</sup>, or other ion types, can be accumulated and used for quantitation. The quantitation result is returned by CycloBranch in seconds or minutes, contrary to an hours-long manual approach, prone to user-born errors originating from necessary copying among various software environments. Native Bruker, Waters, Thermo, txt, mgf, mzML, and mzXML data formats are supported in CycloBranch, which is freely available at https://ms.biomed.cas.cz/cyclobranch.</p>","PeriodicalId":12007,"journal":{"name":"European Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9294015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}