Pub Date : 2024-01-01Epub Date: 2023-11-20DOI: 10.1007/s10493-023-00865-5
Mateus C Matos, Francisco W B Silva, Rosenya M C Filgueiras, Debora B Lima, Jose W S Melo
Multiple arthropod pests can affect the same crop in agricultural systems, requiring the integration of control methods. In the present study, the effects of residual exposure to four broad-spectrum insecticides/acaricides (azadiractin, abamectin, chlorfenapyr, and fenpyroximate) on immature (development and survival time) and adult females (longevity, fecundity, and fertility life table parameters) of the predatory mite Neoseiulus barkeri were evaluated. Additionally, the insecticides/acaricides were categorized according to their selectivity based on the classification proposed by the International Organization for Biological Control (IOBC) for assessing the susceptibility of arthropods in laboratory experiments. Method 004, proposed by the Insecticide Resistance Action Committee (IRAC), was adopted for the bioassays with predators exposed to insecticide-acaricide residues. Among the insecticides/acaricides studied, azadirachtin had minimal effects on immature and adult N. barkeri (all non-significant) and was considered harmless based on the classification of toxicity according to the standards/categories proposed by the IOBC. All other insecticides/acaricides affected immature and adult N. barkeri and were considered slightly harmful in terms of toxicity, according to the IOBC.
{"title":"Compatibility of pesticides with the predatory mite Neoseiulus barkeri.","authors":"Mateus C Matos, Francisco W B Silva, Rosenya M C Filgueiras, Debora B Lima, Jose W S Melo","doi":"10.1007/s10493-023-00865-5","DOIUrl":"10.1007/s10493-023-00865-5","url":null,"abstract":"<p><p>Multiple arthropod pests can affect the same crop in agricultural systems, requiring the integration of control methods. In the present study, the effects of residual exposure to four broad-spectrum insecticides/acaricides (azadiractin, abamectin, chlorfenapyr, and fenpyroximate) on immature (development and survival time) and adult females (longevity, fecundity, and fertility life table parameters) of the predatory mite Neoseiulus barkeri were evaluated. Additionally, the insecticides/acaricides were categorized according to their selectivity based on the classification proposed by the International Organization for Biological Control (IOBC) for assessing the susceptibility of arthropods in laboratory experiments. Method 004, proposed by the Insecticide Resistance Action Committee (IRAC), was adopted for the bioassays with predators exposed to insecticide-acaricide residues. Among the insecticides/acaricides studied, azadirachtin had minimal effects on immature and adult N. barkeri (all non-significant) and was considered harmless based on the classification of toxicity according to the standards/categories proposed by the IOBC. All other insecticides/acaricides affected immature and adult N. barkeri and were considered slightly harmful in terms of toxicity, according to the IOBC.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01Epub Date: 2023-11-28DOI: 10.1007/s10493-023-00866-4
Souad Hazam, Salem Touati, Lounis Touati, Liza Saher, Hassiba Khedidji, Sabrina Ait Kaki, Smain Chemat
Varroosis induced by Varroa destructor Anderson and Trueman represents the most pathogenic and destructive disease affecting the western honey bee, Apis mellifera. In this study, we investigated the acaricidal activity against the Varroa mite using essential oils (EOs) from the aerial parts of four autochthonous Algerian herbal species, namely Artemisia herba alba, Artemisia campestris, Artemisia judaica and Ruta montana. EOs were obtained by means of hydrodistillation and their composition was characterized by gas chromatography-mass spectrometry. The toxicity of the selected EOs toward V. destructor and A. mellifera adult honey bees was evaluated using the complete exposure method. The results indicate the predominance of davanone (66.9%) in A. herba alba, β-pinene (19.5%) in A. campestris, piperitone (68.7%) in A. judaica and 2-undecanone (70.1%) in R. montana EOs. Interestingly, the LC50 values coupled to bee mortality rates revealed that all tested oils exhibited significant acaricidal efficiency with selectivity ratio (SR) values of 10.77, 8.78, 5.62 and 3.73 for A. campestris, A. judaica, A. herba alba, and R. montana, respectively. These values were better than that of thymol (SR = 3.65), the positive control. These findings suggest that these EOs could be used as plant-derived veterinary acaricides to control varroosis in field conditions.
{"title":"Promising Algerian essential oils as natural acaricides against the honey bee mite Varroa destructor (Acari: Varroidae).","authors":"Souad Hazam, Salem Touati, Lounis Touati, Liza Saher, Hassiba Khedidji, Sabrina Ait Kaki, Smain Chemat","doi":"10.1007/s10493-023-00866-4","DOIUrl":"10.1007/s10493-023-00866-4","url":null,"abstract":"<p><p>Varroosis induced by Varroa destructor Anderson and Trueman represents the most pathogenic and destructive disease affecting the western honey bee, Apis mellifera. In this study, we investigated the acaricidal activity against the Varroa mite using essential oils (EOs) from the aerial parts of four autochthonous Algerian herbal species, namely Artemisia herba alba, Artemisia campestris, Artemisia judaica and Ruta montana. EOs were obtained by means of hydrodistillation and their composition was characterized by gas chromatography-mass spectrometry. The toxicity of the selected EOs toward V. destructor and A. mellifera adult honey bees was evaluated using the complete exposure method. The results indicate the predominance of davanone (66.9%) in A. herba alba, β-pinene (19.5%) in A. campestris, piperitone (68.7%) in A. judaica and 2-undecanone (70.1%) in R. montana EOs. Interestingly, the LC<sub>50</sub> values coupled to bee mortality rates revealed that all tested oils exhibited significant acaricidal efficiency with selectivity ratio (SR) values of 10.77, 8.78, 5.62 and 3.73 for A. campestris, A. judaica, A. herba alba, and R. montana, respectively. These values were better than that of thymol (SR = 3.65), the positive control. These findings suggest that these EOs could be used as plant-derived veterinary acaricides to control varroosis in field conditions.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138444387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01Epub Date: 2023-12-12DOI: 10.1007/s10493-023-00872-6
Livia Maria Silva Ataide, Jaqueline F Della Vechia, Ronald Ochoa, Daniel Carrillo, Alexandra M Revynthi
The lychee erinose mite, Aceria litchii (Keifer), is a tiny eriophyid mite known to induce the formation of open galls (erinea) on lychee plants, Litchi chinensis Sonn. In lychee infested by A. litchii, four stages of erineum are observed, based on erineum color: hyaline, white, amber and dark brown. The cause of the color change in erinea is unknown, but it might be linked to the extent of A. litchii infestation. Artificially infested lychee seedlings were used to determine the developmental time, trichome density, and mite population size in each of the four erineum stages. The effect of temperature (15, 25, and 35 °C) on A. litchii population size and erineum developmental time was also investigated. Overall, each erineum stage lasted approximately 50 days and the population size of A. litchii increased gradually through the hyaline, white and amber stages, and a strong decline was observed in dark brown erinea. Visual comparisons of the low-temperature scanning electron microscope (LT-SEM) images revealed that the trichome density was lower in the hyaline stage than in the other stages. Temperature influenced mite population size and amber erinea developmental time. Mite population was lower at 35 °C than at 15 and 25 °C, suggesting that high temperatures promote detrimental effects on A. litchii. Amber erinea developed slower at 15 °C than at 25 and 35 °C. Possible effects of the temperature on A. litchii population in lychee plants are discussed.
{"title":"Influence of temperature on the population size of Aceria litchii (Acari: Eriophyidae) and the development of its galls.","authors":"Livia Maria Silva Ataide, Jaqueline F Della Vechia, Ronald Ochoa, Daniel Carrillo, Alexandra M Revynthi","doi":"10.1007/s10493-023-00872-6","DOIUrl":"10.1007/s10493-023-00872-6","url":null,"abstract":"<p><p>The lychee erinose mite, Aceria litchii (Keifer), is a tiny eriophyid mite known to induce the formation of open galls (erinea) on lychee plants, Litchi chinensis Sonn. In lychee infested by A. litchii, four stages of erineum are observed, based on erineum color: hyaline, white, amber and dark brown. The cause of the color change in erinea is unknown, but it might be linked to the extent of A. litchii infestation. Artificially infested lychee seedlings were used to determine the developmental time, trichome density, and mite population size in each of the four erineum stages. The effect of temperature (15, 25, and 35 °C) on A. litchii population size and erineum developmental time was also investigated. Overall, each erineum stage lasted approximately 50 days and the population size of A. litchii increased gradually through the hyaline, white and amber stages, and a strong decline was observed in dark brown erinea. Visual comparisons of the low-temperature scanning electron microscope (LT-SEM) images revealed that the trichome density was lower in the hyaline stage than in the other stages. Temperature influenced mite population size and amber erinea developmental time. Mite population was lower at 35 °C than at 15 and 25 °C, suggesting that high temperatures promote detrimental effects on A. litchii. Amber erinea developed slower at 15 °C than at 25 and 35 °C. Possible effects of the temperature on A. litchii population in lychee plants are discussed.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138801028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01Epub Date: 2024-01-14DOI: 10.1007/s10493-023-00874-4
Marina Winter, Patrick Stephan Sebastian, Evelina Luisa Tarragona, Fernando Sebastián Flores, Sergio Damián Abate, Santiago Nava
This study presents the results of the molecular detection of tick-borne microorganisms in Amblyomma tigrinum Koch collected near the city of Viedma, Río Negro, Argentina. Ticks were collected in their non-parasitic stage, on pet dogs and on Lycalopex gymnocercus (Pampa fox). Also, six tick samples from humans were analyzed. All ticks were morphologically identified to species level and genomic DNA was extracted. The DNA samples were examined by end point PCR assays to amplified DNA of Anaplasma sp., Babesia sp., Ehrlichia sp., Rickettsia sp. and Theileria sp. Although all tested DNA samples from the collected ticks resulted negative to the detection of Piroplasmida and Rickettsia spp., 16 samples (16.5%, including all hosts) were positive in the 16S rDNA gene PCR that detects bacteria from the Anaplasmataceae family. Phylogenetic analysis of seven obtained partial sequences resulted in the identification of three bacteria: two Ehrlichia spp. (related to Ehrlichia sp. strain Iberá and strain Viedma) and Candidatus Anaplasma boleense. The latter finding represents the first detection of this novel Candidatus species in A. tigrinum. Based on the results of this study, it must be assumed that the diversity of bacteria of the Anaplasmataceae family in Argentina is greater than previously thought, and that these bacteria can infect a wide range of domestic and wild animals.
{"title":"Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina.","authors":"Marina Winter, Patrick Stephan Sebastian, Evelina Luisa Tarragona, Fernando Sebastián Flores, Sergio Damián Abate, Santiago Nava","doi":"10.1007/s10493-023-00874-4","DOIUrl":"10.1007/s10493-023-00874-4","url":null,"abstract":"<p><p>This study presents the results of the molecular detection of tick-borne microorganisms in Amblyomma tigrinum Koch collected near the city of Viedma, Río Negro, Argentina. Ticks were collected in their non-parasitic stage, on pet dogs and on Lycalopex gymnocercus (Pampa fox). Also, six tick samples from humans were analyzed. All ticks were morphologically identified to species level and genomic DNA was extracted. The DNA samples were examined by end point PCR assays to amplified DNA of Anaplasma sp., Babesia sp., Ehrlichia sp., Rickettsia sp. and Theileria sp. Although all tested DNA samples from the collected ticks resulted negative to the detection of Piroplasmida and Rickettsia spp., 16 samples (16.5%, including all hosts) were positive in the 16S rDNA gene PCR that detects bacteria from the Anaplasmataceae family. Phylogenetic analysis of seven obtained partial sequences resulted in the identification of three bacteria: two Ehrlichia spp. (related to Ehrlichia sp. strain Iberá and strain Viedma) and Candidatus Anaplasma boleense. The latter finding represents the first detection of this novel Candidatus species in A. tigrinum. Based on the results of this study, it must be assumed that the diversity of bacteria of the Anaplasmataceae family in Argentina is greater than previously thought, and that these bacteria can infect a wide range of domestic and wild animals.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-21DOI: 10.1007/s10493-023-00869-1
Abstract
Mites are considered a threat to the commercial poultry industry due to their direct and indirect pathogenic effects. Dermanyssus gallinae is among the mite species that endanger production, as it is hematophagous and poses a serious sanitary risk. Recently, Tyrophagus putrescentiae has also drawn attention as a potential pest in the poultry industry, and its attacks have been related to clinical symptoms such as cutaneous eruptions and bird restlessness. Considering the potential economic, sanitary, and productive losses both species represent to the laying-hen industry, the aim of the present study was to evaluate the influence of D. gallinae and T. putrescentiae on the health and productivity of commercial laying hens by carrying out infestation experiments and by monitoring laying hens for a period of 60 days. Tyrophagus putrescentiae and D. gallinae affected egg quality and zootechnical parameters in laying hens. Hens infested by T. putrescentiae had behavioral changes, weight loss, stress symptoms, and decreased egg laying and quality, as well as diarrhea, gradual feather loss, and itching. Hens infested by D. gallinae also had weight loss, behavioral changes, and stress symptoms, leading to decreased laying and egg quality. The present study proposes an experimental model to understand the impacts caused by T. putrescentiae and D. gallinae on commercial laying production and egg quality parameters.
{"title":"Impact of the mites Dermanyssus gallinae and Tyrophagus putrescentiae on production and health of laying hens","authors":"","doi":"10.1007/s10493-023-00869-1","DOIUrl":"https://doi.org/10.1007/s10493-023-00869-1","url":null,"abstract":"<h3>Abstract</h3> <p>Mites are considered a threat to the commercial poultry industry due to their direct and indirect pathogenic effects. <em>Dermanyssus gallinae</em> is among the mite species that endanger production, as it is hematophagous and poses a serious sanitary risk. Recently, <em>Tyrophagus putrescentiae</em> has also drawn attention as a potential pest in the poultry industry, and its attacks have been related to clinical symptoms such as cutaneous eruptions and bird restlessness. Considering the potential economic, sanitary, and productive losses both species represent to the laying-hen industry, the aim of the present study was to evaluate the influence of <em>D. gallinae</em> and <em>T. putrescentiae</em> on the health and productivity of commercial laying hens by carrying out infestation experiments and by monitoring laying hens for a period of 60 days. <em>Tyrophagus putrescentiae</em> and <em>D. gallinae</em> affected egg quality and zootechnical parameters in laying hens. Hens infested by <em>T. putrescentiae</em> had behavioral changes, weight loss, stress symptoms, and decreased egg laying and quality, as well as diarrhea, gradual feather loss, and itching. Hens infested by <em>D. gallinae</em> also had weight loss, behavioral changes, and stress symptoms, leading to decreased laying and egg quality. The present study proposes an experimental model to understand the impacts caused by <em>T. putrescentiae</em> and <em>D. gallinae</em> on commercial laying production and egg quality parameters.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138823557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.1007/s10493-023-00868-2
Zhuo Li, Liang Wang, Tianci Yi, Dongdong Liu, Gang Li, Dao-Chao Jin
The nuclear receptor gene Ecdysone-induced protein 75 (E75), as the component of ecdysone response genes in the ecdysone signaling pathway, has important regulatory function for insect molting. However, the regulatory function of E75 during the molting process of spider mites is not yet clear. In this study, the expression pattern of E75 in the molting process of the spider mite Tetranychus urticae was analyzed. The results showed that there was a peak at 8 h post-molting, followed by a decline 8 h after entering each respective quiescent stage across various developmental stages. During the deutonymph stage, the expression dynamics of E75, observed at 4-h intervals, indicated that the transcript levels of TuE75 peaked at 24 h, coinciding with the onset of molting in the mites. To investigate the function of TuE75 during the molting process, silencing TuE75 through dsRNA injection into deutonymph mites at the age of 8 h yielded a notable outcome: 78% of the deutonymph mites were unable to progress to the adult stage. Among these phenotypic mites, 37% were incapable of transitioning into the quiescent state and eventually succumbed after a certain period. An additional 41% of the mites successfully entered the quiescent state but encountered difficulties in shedding the old epidermis, leading to eventual mortality. In summary, these results suggested that TuE75 plays a key role in the molting process of T. urticae.
{"title":"The nuclear receptor gene E75 plays a key role in regulating the molting process of the spider mite, Tetranychus urticae","authors":"Zhuo Li, Liang Wang, Tianci Yi, Dongdong Liu, Gang Li, Dao-Chao Jin","doi":"10.1007/s10493-023-00868-2","DOIUrl":"https://doi.org/10.1007/s10493-023-00868-2","url":null,"abstract":"<p>The nuclear receptor gene <i>Ecdysone-induced protein 75</i> (<i>E75</i>), as the component of ecdysone response genes in the ecdysone signaling pathway, has important regulatory function for insect molting. However, the regulatory function of <i>E75</i> during the molting process of spider mites is not yet clear. In this study, the expression pattern of <i>E75</i> in the molting process of the spider mite <i>Tetranychus urticae</i> was analyzed. The results showed that there was a peak at 8 h post-molting, followed by a decline 8 h after entering each respective quiescent stage across various developmental stages. During the deutonymph stage, the expression dynamics of <i>E75</i>, observed at 4-h intervals, indicated that the transcript levels of TuE75 peaked at 24 h, coinciding with the onset of molting in the mites. To investigate the function of <i>TuE75</i> during the molting process, silencing <i>TuE75</i> through dsRNA injection into deutonymph mites at the age of 8 h yielded a notable outcome: 78% of the deutonymph mites were unable to progress to the adult stage. Among these phenotypic mites, 37% were incapable of transitioning into the quiescent state and eventually succumbed after a certain period. An additional 41% of the mites successfully entered the quiescent state but encountered difficulties in shedding the old epidermis, leading to eventual mortality. In summary, these results suggested that <i>TuE75</i> plays a key role in the molting process of <i>T. urticae</i>.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.1007/s10493-023-00871-7
Ellie M. S. P. van Dalen, Candice Jansen van Rensburg
Rhipicephalus (Boophilus) microplus, an invasive species to Africa, and the endemic R. (B.) decoloratus are of high economic importance in the cattle industry. Invasion of the alien species in South Africa has mostly been reported for traditional communal grazing areas where it seemed to be rapid and, in some cases, even replaced the native species. The alien species is also assumed to already be resistant to acaricides upon invasion. The presence of R. (B.) microplus on commercial farms was therefore investigated and resistance screening of both species to field concentrations of cypermethrin, amitraz, and chlorfenvinphos was determined by means of the larval immersion test. Results showed that only 3.7% (of 383) tick collections submitted were R. (B.) microplus populations. A further 1.6% (of 383) showed co-existence of the two species. Comparing the level of resistance to the acaricides between the two species indicated a mean phenotypic resistance of 66.2 and 26.5% of R. (B.) decoloratus populations to cypermethrin and amitraz, respectively. This was significantly lower for R. (B.) microplus, with 23.0 and 4.1% of its populations resistant to cypermethrin and amitraz, respectively. Closed commercial farming areas seemed to have a preventative advantage for the invasion of R. (B.) microplus and displacement of R. (B.) decoloratus by R. (B.) microplus. Regular monitoring of these two species may be of high importance to prevent unnecessary financial losses due to insufficient control and increased awareness of the threat of Asiatic babesiosis vectored by R. (B.) microplus.
{"title":"Competitive displacement and acaricide resistance of two Rhipicephalus (Boophilus) species collected on commercial farms in South Africa","authors":"Ellie M. S. P. van Dalen, Candice Jansen van Rensburg","doi":"10.1007/s10493-023-00871-7","DOIUrl":"https://doi.org/10.1007/s10493-023-00871-7","url":null,"abstract":"<p><i>Rhipicephalus</i> (<i>Boophilus</i>) <i>microplus</i>, an invasive species to Africa, and the endemic<i> R.</i> (<i>B.</i>) <i>decoloratus</i> are of high economic importance in the cattle industry. Invasion of the alien species in South Africa has mostly been reported for traditional communal grazing areas where it seemed to be rapid and, in some cases, even replaced the native species. The alien species is also assumed to already be resistant to acaricides upon invasion. The presence of <i>R.</i> (<i>B.</i>) <i>microplus</i> on commercial farms was therefore investigated and resistance screening of both species to field concentrations of cypermethrin, amitraz, and chlorfenvinphos was determined by means of the larval immersion test. Results showed that only 3.7% (of 383) tick collections submitted were <i>R.</i> (<i>B.</i>) <i>microplus</i> populations. A further 1.6% (of 383) showed co-existence of the two species. Comparing the level of resistance to the acaricides between the two species indicated a mean phenotypic resistance of 66.2 and 26.5% of <i>R.</i> (<i>B.</i>) <i>decoloratus</i> populations to cypermethrin and amitraz, respectively. This was significantly lower for <i>R.</i> (<i>B.</i>) <i>microplus</i>, with 23.0 and 4.1% of its populations resistant to cypermethrin and amitraz, respectively. Closed commercial farming areas seemed to have a preventative advantage for the invasion of <i>R.</i> (<i>B.</i>) <i>microplus</i> and displacement of <i>R.</i> (<i>B.</i>) <i>decoloratus</i> by <i>R.</i> (<i>B.</i>) <i>microplus</i>. Regular monitoring of these two species may be of high importance to prevent unnecessary financial losses due to insufficient control and increased awareness of the threat of Asiatic babesiosis vectored by <i>R.</i> (<i>B.</i>) <i>microplus.</i></p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138744361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-12DOI: 10.1007/s10493-023-00864-6
Matthew E. M. Yunik, Chulantha P. Diyes, Neil B. Chilton
A comparison was made of the supercooling points (SCPs) of questing Dermacentor variabilis adults from two populations located ca. 800 km apart on the Canadian prairies. This is also the first study to examine whether there are seasonal fluctuations in the SCP of questing D. variabilis adults. The SCPs of adult ticks from Lizard Lake Community Pasture, a recently established population in west-central Saskatchewan, varied over spring and summer, with the median SCP warming over time. In addition, the SCPs of ticks from Lizard Lake Community Pasture were significantly higher than those of adult ticks collected from Sandy Hook in Manitoba, a population that has been established for decades. The off-host survival of adults from Sandy Hook between summer and spring has been shown previously to be significantly greater than that of adults from Lizard Lake Community Pasture. The findings of the present study suggest that there may be geographical variation in the SCPs of D. variabilis adults which may be associated with differences in overwinter survival. The relatively low SCPs of questing D. variabilis adults, and the ability of some adults to survive off-host during winter, may be factors contributing to the range expansion of this tick species in Canada.
{"title":"Comparison of the supercooling points of questing Dermacentor variabilis adults in two populations on the Canadian prairies and implications for overwinter survival","authors":"Matthew E. M. Yunik, Chulantha P. Diyes, Neil B. Chilton","doi":"10.1007/s10493-023-00864-6","DOIUrl":"https://doi.org/10.1007/s10493-023-00864-6","url":null,"abstract":"<p>A comparison was made of the supercooling points (SCPs) of questing <i>Dermacentor variabilis</i> adults from two populations located ca. 800 km apart on the Canadian prairies. This is also the first study to examine whether there are seasonal fluctuations in the SCP of questing <i>D. variabilis</i> adults. The SCPs of adult ticks from Lizard Lake Community Pasture, a recently established population in west-central Saskatchewan, varied over spring and summer, with the median SCP warming over time. In addition, the SCPs of ticks from Lizard Lake Community Pasture were significantly higher than those of adult ticks collected from Sandy Hook in Manitoba, a population that has been established for decades. The off-host survival of adults from Sandy Hook between summer and spring has been shown previously to be significantly greater than that of adults from Lizard Lake Community Pasture. The findings of the present study suggest that there may be geographical variation in the SCPs of <i>D. variabilis</i> adults which may be associated with differences in overwinter survival. The relatively low SCPs of questing <i>D. variabilis</i> adults, and the ability of some adults to survive off-host during winter, may be factors contributing to the range expansion of this tick species in Canada.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138628661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-31DOI: 10.1007/s10493-023-00860-w
Rui Zhu, Jian-Jun Guo, Tian-Ci Yi, Fei Hou, Dao-Chao Jin
Mass rearing of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) using natural (prey) methods is costly and laborious, limiting its application in the biological control of pests. A high-production, low-cost method using a prey substitute would help to relieve this problem. Oulenziella bakeri Hughes (Acari: Winterschmidtiidae) could be an alternative prey source, but studies on the reproductive parameters of N. californicus under rearing conditions are lacking. This study evaluated the potential of O. bakeri as an alternative prey in N. californicus rearing by comparing developmental parameters among N. californicus reared on three diets based on an age-stage two-sex life table. We found that the preoviposition period and developmental time of N. californicus did not vary based on diet. The fecundity of N. californicus adults reared on O. bakeri was 29.8 eggs per female, which was lower than that of adults reared on Tetranychus urticae Koch (Acari: Tetranychidae) (42.9 eggs per female); there was no significant difference between O. bakeri and apple pollen (30.2 eggs per female). The oviposition rate of mites fed on O. bakeri was 69% of that fed on T. urticae. Neoseiulus californicus reared on O. bakeri and apple pollen showed the same intrinsic rate of increase (0.25 per day), which was 86% of the rate of those fed on T. urticae. Compared with predatory mites reared on natural prey, N. californicus reared on O. bakeri had a high survival rate and good oviposition and population growth parameters, suggesting that O. bakeri is suitable for the rearing of N. californicus.
{"title":"Potential of a winterschmidtiid prey mite for the production of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae).","authors":"Rui Zhu, Jian-Jun Guo, Tian-Ci Yi, Fei Hou, Dao-Chao Jin","doi":"10.1007/s10493-023-00860-w","DOIUrl":"10.1007/s10493-023-00860-w","url":null,"abstract":"<p><p>Mass rearing of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) using natural (prey) methods is costly and laborious, limiting its application in the biological control of pests. A high-production, low-cost method using a prey substitute would help to relieve this problem. Oulenziella bakeri Hughes (Acari: Winterschmidtiidae) could be an alternative prey source, but studies on the reproductive parameters of N. californicus under rearing conditions are lacking. This study evaluated the potential of O. bakeri as an alternative prey in N. californicus rearing by comparing developmental parameters among N. californicus reared on three diets based on an age-stage two-sex life table. We found that the preoviposition period and developmental time of N. californicus did not vary based on diet. The fecundity of N. californicus adults reared on O. bakeri was 29.8 eggs per female, which was lower than that of adults reared on Tetranychus urticae Koch (Acari: Tetranychidae) (42.9 eggs per female); there was no significant difference between O. bakeri and apple pollen (30.2 eggs per female). The oviposition rate of mites fed on O. bakeri was 69% of that fed on T. urticae. Neoseiulus californicus reared on O. bakeri and apple pollen showed the same intrinsic rate of increase (0.25 per day), which was 86% of the rate of those fed on T. urticae. Compared with predatory mites reared on natural prey, N. californicus reared on O. bakeri had a high survival rate and good oviposition and population growth parameters, suggesting that O. bakeri is suitable for the rearing of N. californicus.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71421984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleuroglyphus ovatus (Acari: Acaridae) is a major pest mite of stored grains that is distributed worldwide. Paeonol, a phenolic component of the essential oil extracted from the Chinese herb Paeonia moutan, possesses a range of biological activities, including antiviral, antifungal and acaricidal activity. This study investigated the bioactivity of paeonol against A. ovatus and its effect on the activity of detoxification enzymes. The bioactivity of paeonol against A. ovatus was determined by contact, fumigation and repellency bioassays, and the mechanism was preliminarily explored via morphological observation of the color changes of mite epidermis and determination of the changing trend of some important enzymes associated with acaricidal efficacy in the mites. The results showed that the median lethal concentration (LC50) in the contact and fumigation bioassays was 9.832 μg/cm2 and 14.827 μg/cm3, respectively, and the acaricidal activity of paeonol was higher under direct contact than under fumigation. Dynamic symptomatology studies registered typical neurotoxicity symptoms including excitation, convulsion and paralysis in A. ovatus treated with paeonol. The enzyme activity of catalase (CAT), nitric oxide synthase (NOS) and glutathione-S-transferase (GST) was higher, whereas the activity of superoxide dismutase (SOD) and acetylcholinesterase (AChE) was lower, compared to the control group. CAT, NOS and GST were activated, whereas SOD and AChE activities were inhibited after paeonol intervention. Our findings suggest paeonol has potent acaricidal activity against A. ovatus and thus may be used as an agent to control the stored-product mite A. ovatus.
卵粉粉螨(Acari: Acaridae)是分布在世界各地的储粮害虫螨。丹皮酚是芍药精油中的酚类成分,具有抗病毒、抗真菌、杀螨等多种生物活性。本文研究了丹皮酚对卵圆蚧的生物活性及其对解毒酶活性的影响。采用接触法、熏蒸法和驱避法测定丹皮酚对卵圆螨的生物活性,并通过螨表皮颜色变化的形态学观察和与杀螨效果相关的重要酶的变化趋势,初步探讨其作用机制。结果表明,接触法和熏蒸法的中位致死浓度(LC50)分别为9.832 μg/cm2和14.827 μg/cm3,直接接触法的杀螨活性高于熏蒸法。动态症状学研究记录了典型的神经毒性症状,包括丹皮酚治疗后的卵田鼠兴奋、抽搐和麻痹。过氧化氢酶(CAT)、一氧化氮合酶(NOS)和谷胱甘肽- s -转移酶(GST)活性均高于对照组,超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)活性低于对照组。丹皮酚干预后,CAT、NOS和GST活性被激活,SOD和AChE活性被抑制。本研究结果提示丹皮酚对卵圆螨有较强的杀螨活性,可作为储藏品卵圆螨的防治药剂。
{"title":"Acaricidal activities of paeonol from Moutan Cortex, dried bark of Paeonia × suffruticosa, against the grain pest mite Aleuroglyphus ovatus (Acari: Acaridae).","authors":"Minghui Zou, Qiqi Xue, Qiao Teng, Qiqi Zhang, Ting Liu, Yuanyuan Li, Jinhong Zhao","doi":"10.1007/s10493-023-00861-9","DOIUrl":"10.1007/s10493-023-00861-9","url":null,"abstract":"<p><p>Aleuroglyphus ovatus (Acari: Acaridae) is a major pest mite of stored grains that is distributed worldwide. Paeonol, a phenolic component of the essential oil extracted from the Chinese herb Paeonia moutan, possesses a range of biological activities, including antiviral, antifungal and acaricidal activity. This study investigated the bioactivity of paeonol against A. ovatus and its effect on the activity of detoxification enzymes. The bioactivity of paeonol against A. ovatus was determined by contact, fumigation and repellency bioassays, and the mechanism was preliminarily explored via morphological observation of the color changes of mite epidermis and determination of the changing trend of some important enzymes associated with acaricidal efficacy in the mites. The results showed that the median lethal concentration (LC<sub>50</sub>) in the contact and fumigation bioassays was 9.832 μg/cm<sup>2</sup> and 14.827 μg/cm<sup>3</sup>, respectively, and the acaricidal activity of paeonol was higher under direct contact than under fumigation. Dynamic symptomatology studies registered typical neurotoxicity symptoms including excitation, convulsion and paralysis in A. ovatus treated with paeonol. The enzyme activity of catalase (CAT), nitric oxide synthase (NOS) and glutathione-S-transferase (GST) was higher, whereas the activity of superoxide dismutase (SOD) and acetylcholinesterase (AChE) was lower, compared to the control group. CAT, NOS and GST were activated, whereas SOD and AChE activities were inhibited after paeonol intervention. Our findings suggest paeonol has potent acaricidal activity against A. ovatus and thus may be used as an agent to control the stored-product mite A. ovatus.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136396950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}