Grace E. Houston, Allison K. Blomme, O. Harrison, J. Bai, J. Woodworth, Cassandra K. Jones, Elizabeth G. Poulsen-Porter, C. Paulk, J. Gebhardt
This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.
{"title":"Evaluating the impact of organic matter and sample processing technique on RNA detection using environmental samples","authors":"Grace E. Houston, Allison K. Blomme, O. Harrison, J. Bai, J. Woodworth, Cassandra K. Jones, Elizabeth G. Poulsen-Porter, C. Paulk, J. Gebhardt","doi":"10.54846/am2022/106","DOIUrl":"https://doi.org/10.54846/am2022/106","url":null,"abstract":"This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115480827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernando Leite, Paulo Arruda, Dianna Jordan, Shawn Bearson
{"title":"Efficacy of Enterisol Salmonella T/C® in reducing the shedding and colonization of multidrug resistant monophasic <i>Salmonella enterica</i> serovar I 4,[5],12:i:-","authors":"Fernando Leite, Paulo Arruda, Dianna Jordan, Shawn Bearson","doi":"10.54846/am2023/75","DOIUrl":"https://doi.org/10.54846/am2023/75","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"277 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135238866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Veterinary medicine and swine nutrition at JBS","authors":"L. Glowzenski","doi":"10.54846/am2023/182","DOIUrl":"https://doi.org/10.54846/am2023/182","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"193 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135238859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Veterinary medicine and swine nutrition at AMVC","authors":"J. Hocker, T. Kellner","doi":"10.54846/am2023/183","DOIUrl":"https://doi.org/10.54846/am2023/183","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135239113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vaccination with an <i>Escherichia coli</i> F4/F18 vaccine improves piglet performance combined with a reduction in antimicrobial use and secondary infections due to <i>Streptococcus suis</i>","authors":"Frédéric Vangroenweghe, Mieke Boone","doi":"10.54846/am2023/133","DOIUrl":"https://doi.org/10.54846/am2023/133","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135238856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Balka, L. Schwarz, R. Brunthaler, S. Högler, Lilla Dénes
{"title":"Detection and localization of atypical porcine pestivirus in the reproductive tract of a persistently infected boar","authors":"G. Balka, L. Schwarz, R. Brunthaler, S. Högler, Lilla Dénes","doi":"10.54846/am2023/108","DOIUrl":"https://doi.org/10.54846/am2023/108","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117183125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DFM PAK™ improves manure characteristics to influence environmental and health benefits in the pit, the pen and in the air","authors":"J. Lee, J. Spencer, E. Galbraith, S. Son, M. King","doi":"10.54846/am2023/69","DOIUrl":"https://doi.org/10.54846/am2023/69","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"9 13","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120965424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative efficacy of Aivlosin® and tilmicosin for treatment of swine respiratory disease in nursery-age pigs","authors":"C. Sexton, C. Smith, N. Lauterbach, J. Okones","doi":"10.54846/am2023/57","DOIUrl":"https://doi.org/10.54846/am2023/57","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127110047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Retrospective analysis of Mycoplasma hyorhinis pulmonary and systemic infection in diagnostic cases with correlation of qPCR Ct values and detection by RNAscope®","authors":"M. Merodio, J. Groeltz, P. Piñeyro, R. Derscheid","doi":"10.54846/am2022/121","DOIUrl":"https://doi.org/10.54846/am2022/121","url":null,"abstract":"","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126865784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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