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PCR diagnostic platforms for non-Aspergillus mold infections: ready for routine implementation in the clinic? 非曲霉菌霉菌感染的 PCR 诊断平台:是否已准备好在临床上常规使用?
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2024-03-19 DOI: 10.1080/14737159.2024.2326474
Frederic Lamoth, Dimitrios P Kontoyiannis

Introduction: While Aspergillus spp. remain the predominant cause of invasive mold infections, non-Aspergillus molds, such as the Mucorales or Fusarium spp., account for an increasing proportion of cases. The diagnosis of non-Aspergillus invasive mold infections (NAIMI) is challenging because of the low sensitivity and delay of conventional microbiological tests. Therefore, there is a particular interest to develop molecular tools for their early detection in blood or other clinical samples.

Areas covered: This extensive review of the literature discusses the performance of Mucorales-specific PCR and other genus-specific or broad-range fungal PCR that can be used for the diagnosis of NAIMI in diverse clinical samples, with a focus on novel technologies.

Expert opinion: PCR currently represents the most promising approach, combining good sensitivity/specificity and ability to detect NAIMI in clinical samples before diagnosis by conventional cultures and histopathology. Several PCR assays have been designed for the detection of Mucorales in particular, but also Fusarium spp. or Scedosporium/Lomentospora spp. Some commercial Mucorales PCRs are now available. While efforts are still needed for standardized protocols and the development of more rapid and simpler techniques, PCR is on the way to becoming an essential test for the early diagnosis of mucormycosis and possibly other NAIMIs.

导言:虽然曲霉菌属仍然是侵袭性霉菌感染的主要病因,但非曲霉菌霉菌(如粘菌或镰刀菌属)在病例中所占的比例也在不断增加。非曲霉菌侵袭性霉菌感染(NAIMI)的诊断极具挑战性,因为传统微生物检验的灵敏度低且延迟。因此,人们特别关注开发分子工具,以便在血液或其他临床样本中对其进行早期检测:这篇广泛的文献综述讨论了黏菌类特异性 PCR 和其他属特异性或广谱真菌 PCR 的性能,这些 PCR 可用于诊断不同临床样本中的非传染性真菌感染,重点关注新型技术:目前,PCR 是最有前途的方法,它具有良好的灵敏度/特异性,能够在传统培养和组织病理学诊断之前检测临床样本中的 NAIMI。目前已设计出几种 PCR 检测方法,特别是用于检测粘孢子菌,也可用于检测镰刀菌属(Fusarium spp.)或孢子菌属(Scedosporium/Lomentospora spp.)。虽然仍需努力制定标准化方案并开发更快速、更简单的技术,但 PCR 正逐渐成为粘孢子菌病以及其他非传染性疾病早期诊断的一种基本检测方法。
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引用次数: 0
The impact of companion diagnostic testing on medical decision making and IVD regulations. 辅助诊断测试对医疗决策和 IVD 法规的影响。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2024-02-12 DOI: 10.1080/14737159.2024.2317976
Jan Trøst Jørgensen
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引用次数: 0
Advancements in protein glycosylation biomarkers for ovarian cancer through mass spectrometry-based approaches. 通过质谱方法研究卵巢癌蛋白质糖基化生物标记物的进展。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2023-12-25 DOI: 10.1080/14737159.2023.2297933
Jua Lee, Ji Eun Park, Daum Lee, Nari Seo, Hyun Joo An

Introduction: Ovarian cancer, characterized by metastasis and reduced 5-year survival rates, stands as a substantial factor in the mortality of gynecological malignancies worldwide. The challenge of delayed diagnosis originates from vague early symptoms and the absence of efficient screening and diagnostic biomarkers for early cancer detection. Recent studies have explored the intricate interplay between ovarian cancer and protein glycosylation, unveiling the potential significance of glycosylation-oriented biomarkers.

Areas covered: This review examines the progress in glycosylation biomarker research, with particular emphasis on advances driven by mass spectrometry-based technologies. We document milestones achieved, discuss encountered limitations, and also highlight potential areas for future research and development of protein glycosylation biomarkers for ovarian cancer.

Expert opinion: The association of glycosylation in ovarian cancer is well known, but current research lacks desired sensitivity and specificity for early detection. Notably, investigations into protein-specific and site-specific glycoproteomics have the potential to significantly enhance our understanding of ovarian cancer and facilitate the identification of glycosylation-based biomarkers. Furthermore, the integration of advanced mass spectrometry techniques with AI-driven analysis and glycome databases holds the promise for revolutionizing biomarker discovery for ovarian cancer, ultimately transforming diagnosis and improving patient outcomes.

导言:卵巢癌以转移和降低 5 年生存率为特征,是全球妇科恶性肿瘤死亡率的一个重要因素。由于早期症状模糊,且缺乏有效的筛查和诊断生物标志物来进行早期癌症检测,这就给延迟诊断带来了挑战。最近的研究探索了卵巢癌与蛋白质糖基化之间错综复杂的相互作用,揭示了以糖基化为导向的生物标志物的潜在意义:这篇综述探讨了糖基化生物标记物研究的进展,特别强调了基于质谱技术的研究进展。我们记录了取得的阶段性成果,讨论了遇到的局限性,还强调了卵巢癌蛋白质糖基化生物标记物未来研究和开发的潜在领域:糖基化与卵巢癌的关系众所周知,但目前的研究缺乏早期检测所需的灵敏度和特异性。值得注意的是,对蛋白质特异性和位点特异性糖蛋白组学的研究有可能大大提高我们对卵巢癌的认识,促进糖基化生物标志物的鉴定。此外,先进的质谱技术与人工智能驱动的分析和糖蛋白数据库的整合有望彻底改变卵巢癌生物标志物的发现,最终改变诊断方法并改善患者预后。
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引用次数: 0
SOWAHB polymorphisms affect thyroid cancer risk in the Chinese Han population. SOWAHB多态性影响中国汉族人群患甲状腺癌的风险。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2024-01-23 DOI: 10.1080/14737159.2024.2305183
Man Zhang, Jing Liang, Junhui Han, Wenjing Zhang, Panpan Wan, Leteng Yang, Xufeng Zang, Wanli Ren, Ling Zhang, Hao Dai, Yue Wu, Tianbo Jin

Objectives: This study aimed to detect the correlation between SOWAHB polymorphisms and Thyroid cancer (TC) risk in the Chinese Han population.

Methods: We genotyped SOWAHB variants in 510 TC patients and 509 controls using Agena MassARRAY. We assessed the association between SOWAHB polymorphisms and TC susceptibility, with the significant results evaluated through FPRP analysis. We predicted TC risk by the SNP-SNP interaction, analyzed by MDR.

Results: Carriers with rs2703129 CC had a lower probability of TC (codominant, recessive: p = 0.002), while subjects with rs1874564 AG had an increased risk of developing TC (codominant, recessive: p = 0.000, log-additive: p = 0.028). In subjects aged > 45 years, rs2703129 may reduce TC predisposition (codominant: p = 0.011, recessive: p = 0.007), but there was an increased association between rs1874564 and TC risk (codominant: p = 0.030, dominant: p = 0.047). Also, rs2703129 was associated with a lower risk of TC among males (codominant: p = 0.018, recessive: p = 0.013). Conversely, rs1874564 was associated with an increased risk of TC in females (codominant: p = 0.001, dominant: p = 0.003).

Conclusion: SOWAHB SNPs were related to the occurrence of TC, and rs2703129 may be a protective site for TC.

研究目的本研究旨在检测中国汉族人群中SOWAHB多态性与甲状腺癌(TC)风险之间的相关性:方法:我们使用Agena MassARRAY对510名TC患者和509名对照者的SOWAHB变异进行了基因分型。我们评估了 SOWAHB 多态性与 TC 易感性之间的关联,并通过 FPRP 分析评估了显著结果。我们通过MDR分析SNP-SNP相互作用来预测TC风险:结果:rs2703129 CC携带者患TC的概率较低(显性、隐性:p = 0.002),而rs1874564 AG携带者患TC的风险较高(显性、隐性:p = 0.000,对数加成:p = 0.028)。在年龄大于 45 岁的受试者中,rs2703129 可降低 TC 易感性(显性:p = 0.011,隐性:p = 0.007),但 rs1874564 与 TC 风险之间的关联性增加(显性:p = 0.030,显性:p = 0.047)。此外,rs2703129 与男性 TC 风险较低有关(显性:p = 0.018,隐性:p = 0.013)。相反,rs1874564 与女性 TC 风险增加有关(共显性:p = 0.001,显性:p = 0.003):结论:SOWAHB SNPs与TC的发生有关,rs2703129可能是TC的保护位点。
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引用次数: 0
Detection of lung cancer and stages via breath analysis using a self-made electronic nose device. 使用自制的电子鼻装置,通过呼气分析检测肺癌及其分期。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2024-02-19 DOI: 10.1080/14737159.2024.2316755
Binson V A, Philip Mathew, Sania Thomas, Luke Mathew

Background: Breathomics is an emerging area focusing on monitoring and diagnosing pulmonary diseases, especially lung cancer. This research aims to employ metabolomic methods to create a breathprint in human-expelled air to rapidly identify lung cancer and its stages.

Research design and methods: An electronic nose (e-nose) system with five metal oxide semiconductor (MOS) gas sensors, a microcontroller, and machine learning algorithms was designed and developed for this application. The volunteers in this study include 114 patients with lung cancer and 147 healthy controls to understand the clinical potential of the e-nose system to detect lung cancer and its stages.

Results: In the training phase, in discriminating lung cancer from controls, the XGBoost classifier model with 10-fold cross-validation gave an accuracy of 91.67%. In the validation phase, the XGBoost classifier model correctly identified 35 out of 42 patients with lung cancer samples and 44 out of 51 healthy control samples providing an overall sensitivity of 83.33% and specificity of 86.27%.

Conclusions: These results indicate that the exhaled breath VOC analysis method may be developed as a new diagnostic tool for lung cancer detection. The advantages of e-nose based diagnostics, such as an easy and painless method of sampling, and low-cost procedures, will make it an excellent diagnostic method in the future.

背景:呼吸组学是一个新兴领域,主要用于监测和诊断肺部疾病,尤其是肺癌。本研究旨在利用代谢组学方法在人体排出的空气中建立呼吸指纹,以快速识别肺癌及其分期:研究设计和开发了一个电子鼻(e-nose)系统,其中包含五个金属氧化物半导体(MOS)气体传感器、一个微控制器和机器学习算法。这项研究的志愿者包括 114 名肺癌患者和 147 名健康对照者,目的是了解电子鼻系统检测肺癌及其分期的临床潜力:在训练阶段,XGBoost 分类器模型在区分肺癌和对照组时的准确率为 91.67%。在验证阶段,XGBoost 分类器模型正确识别了 42 个肺癌患者样本中的 35 个样本和 51 个健康对照样本中的 44 个样本,总体灵敏度为 83.33%,特异度为 86.27%:这些结果表明,呼出气体挥发性有机化合物分析方法可作为一种新的肺癌检测诊断工具。基于电子鼻的诊断方法具有取样简便、无痛苦、成本低廉等优点,未来将成为一种很好的诊断方法。
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引用次数: 0
Body fluid markers for multiple sclerosis and differential diagnosis from atypical demyelinating disorders. 多发性硬化症的体液标志物以及与非典型脱髓鞘疾病的鉴别诊断。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-04-01 Epub Date: 2024-03-27 DOI: 10.1080/14737159.2024.2334849
Angelika Bauer, Harald Hegen, Markus Reindl

Introduction: Body fluid markers could be helpful to predict the conversion into clinically definite multiple sclerosis (MS) in people with a first demyelinating event of the central nervous system (CNS). Consequently, biomarkers such as oligoclonal bands, which are integrated in the current MS diagnostic criteria, could assist early MS diagnosis.

Areas covered: This review examines existing knowledge on a broad spectrum of body fluid markers in people with a first CNS demyelinating event, explores their potential to predict conversion to MS, to assess MS disease activity, as well as their utility to differentiate MS from atypical demyelinating disorders such as neuromyelitis optica spectrum disorder and myelin oligodendrocyte glycoprotein associated disease.

Expert opinion: This field of research has shown a dramatic increase of evidence, especially in the last decade. Some biomarkers are already established in clinical routine (e.g. oligoclonal bands) while others are currently implemented (e.g. kappa free light chains) or considered as breakthroughs (e.g. neurofilament light). Determination of biomarkers poses challenges for continuous monitoring, especially if exclusively detectable in cerebrospinal fluid. A handful of biomarkers are measurable in blood which holds a significant potential.

导言体液标志物有助于预测中枢神经系统(CNS)首次出现脱髓鞘症状的患者是否会转变为临床明确的多发性硬化症(MS)。因此,已纳入现行多发性硬化症诊断标准的寡克隆带等生物标志物有助于早期诊断多发性硬化症:本综述研究了首次中枢神经系统脱髓鞘事件患者体液标志物的现有知识,探讨了这些标志物在预测多发性硬化症转归、评估多发性硬化症疾病活动性方面的潜力,以及它们在区分多发性硬化症与非典型脱髓鞘疾病(如神经性脊髓炎视网膜谱系障碍和髓鞘少突胶质细胞糖蛋白相关疾病)方面的效用:这一研究领域的证据急剧增加,尤其是在过去十年中。一些生物标志物已在临床常规中确立(如少克隆带),而另一些生物标志物目前正在实施(如 kappa 游离轻链)或被视为突破性成果(如神经丝光)。生物标记物的确定给持续监测带来了挑战,特别是如果只能在脑脊液中检测到生物标记物。少数几种生物标志物可在血液中测量,具有很大的潜力。
{"title":"Body fluid markers for multiple sclerosis and differential diagnosis from atypical demyelinating disorders.","authors":"Angelika Bauer, Harald Hegen, Markus Reindl","doi":"10.1080/14737159.2024.2334849","DOIUrl":"10.1080/14737159.2024.2334849","url":null,"abstract":"<p><strong>Introduction: </strong>Body fluid markers could be helpful to predict the conversion into clinically definite multiple sclerosis (MS) in people with a first demyelinating event of the central nervous system (CNS). Consequently, biomarkers such as oligoclonal bands, which are integrated in the current MS diagnostic criteria, could assist early MS diagnosis.</p><p><strong>Areas covered: </strong>This review examines existing knowledge on a broad spectrum of body fluid markers in people with a first CNS demyelinating event, explores their potential to predict conversion to MS, to assess MS disease activity, as well as their utility to differentiate MS from atypical demyelinating disorders such as neuromyelitis optica spectrum disorder and myelin oligodendrocyte glycoprotein associated disease.</p><p><strong>Expert opinion: </strong>This field of research has shown a dramatic increase of evidence, especially in the last decade. Some biomarkers are already established in clinical routine (e.g. oligoclonal bands) while others are currently implemented (e.g. kappa free light chains) or considered as breakthroughs (e.g. neurofilament light). Determination of biomarkers poses challenges for continuous monitoring, especially if exclusively detectable in cerebrospinal fluid. A handful of biomarkers are measurable in blood which holds a significant potential.</p>","PeriodicalId":12113,"journal":{"name":"Expert Review of Molecular Diagnostics","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A state-of-the-art overview of candidate diagnostic biomarkers for attention-Deficit/Hyperactivity disorder (ADHD) 注意力缺陷/多动障碍(ADHD)候选诊断生物标志物的最新概况
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-03-20 DOI: 10.1080/14737159.2024.2333277
Valeria Parlatini, Alessio Bellato, Alessandra Gabellone, Lucia Margari, Lucia Marzulli, Emilia Matera, Maria Giuseppina Petruzzelli, Marco Solmi, Christoph U. Correll, Samuele Cortese
Attention-deficit/hyperactivity disorder (ADHD) is one of the most common neurodevelopmental conditions and is highly heterogeneous in terms of symptom profile, associated cognitive deficits, comor...
注意力缺陷/多动障碍(ADHD)是最常见的神经发育疾病之一,在症状特征、相关认知缺陷、共性和个体差异等方面存在很大差异。
{"title":"A state-of-the-art overview of candidate diagnostic biomarkers for attention-Deficit/Hyperactivity disorder (ADHD)","authors":"Valeria Parlatini, Alessio Bellato, Alessandra Gabellone, Lucia Margari, Lucia Marzulli, Emilia Matera, Maria Giuseppina Petruzzelli, Marco Solmi, Christoph U. Correll, Samuele Cortese","doi":"10.1080/14737159.2024.2333277","DOIUrl":"https://doi.org/10.1080/14737159.2024.2333277","url":null,"abstract":"Attention-deficit/hyperactivity disorder (ADHD) is one of the most common neurodevelopmental conditions and is highly heterogeneous in terms of symptom profile, associated cognitive deficits, comor...","PeriodicalId":12113,"journal":{"name":"Expert Review of Molecular Diagnostics","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140167795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Maintaining point of care testing capacity and pandemic preparedness in the post-COVID-19 era. 在后COVID-19时代保持护理点检测能力和大流行准备。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-03-01 Epub Date: 2023-09-24 DOI: 10.1080/14737159.2023.2260743
Jennifer L Rakeman-Cagno, David H Persing, Michael J Loeffelholz

Introduction: Testing at the point of care (we also refer to the 'point of need'), with rapid, actionable results reported to the patient and provider within hours can impact the individual as well as public health. Faster testing is good for patients and public health outcomes during 'peace time' (outside of the pandemic setting).

Areas covered: Testing at the point of need was important during the COVID-19 pandemic to meet testing capacity demands, providing actionable results, and for providing testing within communities to increase access for all populations. Resources were acquired and built up dramatically during the pandemic as part of the response. With the end of the COVID-19 public health emergency and transition back to 'peace time' some testing sites have successfully shifted to using this capacity for testing for other critical needs, like sexually transmitted infection (STI) testing, and response to other seasonal diseases and for outbreak response.

Expert opinion: The increased testing capacity added to handle unprecedented testing volume during the COVID-19 pandemic can be repurposed for other critical infectious diseases during 'peace time' (post-COVID-19 pandemic). This maintains testing capacity for the next pandemic.

引言:在护理点(我们也指“需求点”)进行检测,在数小时内向患者和提供者报告快速、可操作的结果,可以影响个人和公众健康。在“和平时期”(疫情环境之外),更快的检测有利于患者和公共卫生结果。涵盖的领域:在新冠肺炎大流行期间,在需要的时候进行检测对于满足检测能力需求、提供可操作的结果以及在社区内提供检测以增加所有人群的机会至关重要。作为应对措施的一部分,在疫情期间获得并大量积累了资源。随着新冠肺炎公共卫生紧急状态的结束和向“和平时期”的过渡,一些检测点已成功地将这种能力用于检测其他关键需求,如性传播感染(STI)检测,以及应对其他季节性疾病和疫情应对。专家意见:在新冠肺炎大流行期间,为应对前所未有的检测量而增加的检测能力可以在“和平时期”(新冠肺炎大流行后)用于其他关键传染病。这为下一次疫情保持了检测能力。
{"title":"Maintaining point of care testing capacity and pandemic preparedness in the post-COVID-19 era.","authors":"Jennifer L Rakeman-Cagno, David H Persing, Michael J Loeffelholz","doi":"10.1080/14737159.2023.2260743","DOIUrl":"10.1080/14737159.2023.2260743","url":null,"abstract":"<p><strong>Introduction: </strong>Testing at the point of care (we also refer to the 'point of need'), with rapid, actionable results reported to the patient and provider within hours can impact the individual as well as public health. Faster testing is good for patients and public health outcomes during 'peace time' (outside of the pandemic setting).</p><p><strong>Areas covered: </strong>Testing at the point of need was important during the COVID-19 pandemic to meet testing capacity demands, providing actionable results, and for providing testing within communities to increase access for all populations. Resources were acquired and built up dramatically during the pandemic as part of the response. With the end of the COVID-19 public health emergency and transition back to 'peace time' some testing sites have successfully shifted to using this capacity for testing for other critical needs, like sexually transmitted infection (STI) testing, and response to other seasonal diseases and for outbreak response.</p><p><strong>Expert opinion: </strong>The increased testing capacity added to handle unprecedented testing volume during the COVID-19 pandemic can be repurposed for other critical infectious diseases during 'peace time' (post-COVID-19 pandemic). This maintains testing capacity for the next pandemic.</p>","PeriodicalId":12113,"journal":{"name":"Expert Review of Molecular Diagnostics","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10363645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The diagnosis of central nervous system infections in resource-limited settings and the use of novel and molecular diagnostic platforms to improve diagnosis. 在资源有限的环境中诊断中枢神经系统感染,利用新型分子诊断平台改进诊断。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-03-01 Epub Date: 2024-02-19 DOI: 10.1080/14737159.2024.2317414
James Milburn, Rachita Suresh, Ronan Doyle, Joseph N Jarvis

Introduction: Central nervous system infections (CNSI) disproportionately affect individuals in low-resource settings where diagnosis is challenging; large proportions of patients never receive a confirmed microbiological diagnosis resulting in inadequate management and high mortality. The epidemiology of CNSI varies globally and conventional diagnostics deployed in resource-limited settings have significant limitations, with an urgent need for improved diagnostic strategies.

Areas covered: This review describes molecular platforms and other novel diagnostics used in the diagnosis of CNSI that are applicable to resource-limited settings. An extensive literature search of Medline and PubMed was performed. The emphasis is on investigations targeting infections of relevance to resource-limited settings either due to variation in regional CNSI epidemiology or due to increased prevalence in patients with immunosuppression. This includes commercially available multiplex PCR platforms, mycobacterial PCR platforms, and rapid diagnostics tests. To offer a framework for the optimal implementation in clinical settings, existing evidence highlighting the advantages and limitations of available platforms is reviewed.

Expert opinion: The implementation of molecular platforms and other novel diagnostics has the potential to transform CNSI diagnosis in resource-limited settings, with several examples of successful rollout of novel diagnostics such as Xpert MTB/RIF Ultra and cryptococcal antigen testing.

导言:中枢神经系统感染(CNSI)对资源匮乏地区的患者造成的影响尤为严重,因为那里的诊断难度很大;大部分患者从未得到过微生物学确诊,导致治疗不当和死亡率居高不下。CNSI 的流行病学在全球范围内各不相同,在资源有限的环境中使用的传统诊断方法有很大的局限性,因此迫切需要改进诊断策略:本综述介绍了用于诊断 CNSI 的分子平台和其他适用于资源有限环境的新型诊断方法。对 Medline 和 PubMed 进行了广泛的文献检索。重点放在针对与资源有限环境相关的感染的研究上,这些感染或是由于地区性 CNSI 流行病学的变化,或是由于免疫抑制患者患病率的增加。其中包括市售的多重 PCR 平台、分枝杆菌 PCR 平台和快速诊断检测。为了给临床环境中的最佳实施提供一个框架,本文回顾了现有证据,强调了可用平台的优势和局限性:专家意见:分子平台和其他新型诊断方法的应用有可能改变资源有限环境中的 CNSI 诊断,Xpert MTB/RIF Ultra 和隐球菌抗原检测等新型诊断方法已有多个成功推广的实例。
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引用次数: 0
Point-of-care testing for viral-associated pulmonary aspergillosis. 病毒性肺曲霉菌病的护理点检测。
IF 5.1 3区 医学 Q1 Medicine Pub Date : 2024-03-01 Epub Date: 2023-09-21 DOI: 10.1080/14737159.2023.2257597
Robina Aerts, Brice Autier, Maximilian Gornicec, Juergen Prattes, Katrien Lagrou, Jean-Pierre Gangneux, Martin Hoenigl

Introduction: Over the last years, severe respiratory viral infections, particularly those caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the influenza virus, have emerged as risk factor for viral-associated pulmonary aspergillosis (VAPA) among critically ill patients. Delays in diagnosis of VAPA are associated with increased mortality. Point-of-care-tests may play an important role in earlier diagnosis of VAPA and thus improve patient outcomes.

Areas covered: The following review will give an update on point-of-care tests for VAPA, analyzing performances in respiratory and blood specimens.

Expert opinion: Point-of-care tests have emerged, and particularly the IMMY Aspergillus galactomannan lateral flow assay (LFA) shows performances comparable to the galactomannan ELISA for diagnosis of VAPA. Notably, nearly all evaluations of POC tests for VAPA have been performed in COVID-19 patients, with very limited data in influenza patients. For early diagnosis of COVID associated pulmonary aspergillosis (CAPA), the LFA has shown promising performances in respiratory samples, particularly in bronchoalveolar lavage fluid, and may thereby help in improving patient outcomes. In contrast, serum LFA testing may not be useful for early diagnosis of disease, except in cases with invasive tracheobronchial aspergillosis.

引言:在过去几年中,严重呼吸道病毒感染,特别是由严重急性呼吸综合征冠状病毒2型(严重急性呼吸系统综合征冠状病毒冠状病毒2型)和流感病毒引起的感染,已成为危重患者中病毒相关肺曲霉菌病(VAPA)的风险因素。VAPA诊断延迟与死亡率增加有关。护理点测试可能在VAPA的早期诊断中发挥重要作用,从而改善患者的预后。涵盖的领域:以下综述将提供VAPA护理点测试的最新情况,分析呼吸和血液样本的表现。专家意见:已经出现了护理点测试,特别是IMMY曲霉半乳甘露聚糖侧流分析(LFA)在诊断VAPA方面显示出与半乳甘露聚糖ELISA相当的性能。值得注意的是,几乎所有的VAPA POC测试评估都是在新冠肺炎患者中进行的,流感患者的数据非常有限。对于新冠肺炎相关肺曲霉菌病(CAPA)的早期诊断,LFA在呼吸样本中,特别是在支气管肺泡灌洗液中表现出了良好的表现,从而可能有助于改善患者的预后。相反,血清LFA检测可能对疾病的早期诊断无效,除非是侵袭性气管支气管曲霉菌病。
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引用次数: 0
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Expert Review of Molecular Diagnostics
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