Ji-Yeong Park, Min-Jong Kim, Young-Ae Choi, Yeon-Yong Kim, Soyoung Lee, Jae-Min Chung, Sang-Yong Kim, Gil-Saeng Jeong, Sang-Hyun Kim
For centuries, natural products are regarded as vital medicines for human survival. Clematis terniflora var. mandshurica (Rupr.) Ohwi is an ingredient of the herbal medicine, Wei Ling Xian, which has been used in Chinese medicine to alleviate pain, fever, and inflammation. In particular, C. terniflora leaves have been used to cure various inflammatory diseases, including tonsillitis, cholelithiasis, and conjunctivitis. Based on these properties, this study aimed to scientifically investigate the anti-inflammatory effect of an ethanol extract of leaves of C. terniflora (EELCT) using activated macrophages that play central roles in inflammatory response. In this study, EELCT inhibited the essential inflammatory mediators, such as nitric oxide, cyclooxygenase-2, tumor necrosis factor-α, interleukin- (IL-) 6, IL-1β, and inducible nitric oxide synthase, by suppressing the nuclear factor-κB and mitogen-activated protein kinase activation in macrophages. Acute lung injury (ALI) is a fatal respiratory disease accompanied by serious inflammation. With high mortality rate, the disease has no effective treatments. Therefore, new therapeutic agents must be developed for ALI. We expected that EELCT can be a promising therapeutic agent for ALI by reducing inflammatory responses and evaluated its action in a lipopolysaccharide- (LPS-) induced ALI model. EELCT alleviated histological changes, immune cell infiltration, inflammatory mediator production, and protein-rich pulmonary edema during ALI. Collectively, our results may explain the traditional usage of C. terniflora in inflammatory diseases and suggest the promising potential of EELCT as therapeutic candidate for ALI.
几个世纪以来，天然产品一直被视为人类生存的重要药物。Clematis terniflora var. mandshurica (Rupr.) Ohwi 是中药材 "威灵仙 "中的一种成分，在中药中被用来缓解疼痛、发烧和炎症。特别是，三叶草叶被用于治疗各种炎症，包括扁桃体炎、胆石症和结膜炎。基于这些特性，本研究旨在利用在炎症反应中起核心作用的活化巨噬细胞，科学地研究三叶青叶乙醇提取物（EELCT）的抗炎作用。在这项研究中，EELCT通过抑制巨噬细胞中核因子-κB和丝裂原活化蛋白激酶的活化，抑制了一氧化氮、环氧化酶-2、肿瘤坏死因子-α、白细胞介素（IL-）6、IL-1β和诱导型一氧化氮合酶等重要炎症介质。急性肺损伤（ALI）是一种伴随严重炎症的致命性呼吸道疾病。该病死亡率高，却没有有效的治疗方法。因此，必须针对 ALI 开发新的治疗药物。我们预计，EELCT 可通过减轻炎症反应成为治疗 ALI 的有效药物，并在脂多糖（LPS）诱导的 ALI 模型中对其作用进行了评估。EELCT减轻了ALI过程中的组织学变化、免疫细胞浸润、炎症介质产生和富含蛋白质的肺水肿。总之，我们的研究结果可以解释三叶草在炎症性疾病中的传统用法，并表明 EELCT 作为 ALI 候选疗法的潜力巨大。
{"title":"Anti-Inflammatory Effects of Clematis terniflora Leaf on Lipopolysaccharide-Induced Acute Lung Injury","authors":"Ji-Yeong Park, Min-Jong Kim, Young-Ae Choi, Yeon-Yong Kim, Soyoung Lee, Jae-Min Chung, Sang-Yong Kim, Gil-Saeng Jeong, Sang-Hyun Kim","doi":"10.1155/2024/6653893","DOIUrl":"https://doi.org/10.1155/2024/6653893","url":null,"abstract":"For centuries, natural products are regarded as vital medicines for human survival. <i>Clematis terniflora</i> var. <i>mandshurica</i> (Rupr.) Ohwi is an ingredient of the herbal medicine, Wei Ling Xian, which has been used in Chinese medicine to alleviate pain, fever, and inflammation. In particular, <i>C. terniflora</i> leaves have been used to cure various inflammatory diseases, including tonsillitis, cholelithiasis, and conjunctivitis. Based on these properties, this study aimed to scientifically investigate the anti-inflammatory effect of an ethanol extract of leaves of <i>C. terniflora</i> (EELCT) using activated macrophages that play central roles in inflammatory response. In this study, EELCT inhibited the essential inflammatory mediators, such as nitric oxide, cyclooxygenase-2, tumor necrosis factor-<i>α</i>, interleukin- (IL-) 6, IL-1<i>β</i>, and inducible nitric oxide synthase, by suppressing the nuclear factor-<i>κ</i>B and mitogen-activated protein kinase activation in macrophages. Acute lung injury (ALI) is a fatal respiratory disease accompanied by serious inflammation. With high mortality rate, the disease has no effective treatments. Therefore, new therapeutic agents must be developed for ALI. We expected that EELCT can be a promising therapeutic agent for ALI by reducing inflammatory responses and evaluated its action in a lipopolysaccharide- (LPS-) induced ALI model. EELCT alleviated histological changes, immune cell infiltration, inflammatory mediator production, and protein-rich pulmonary edema during ALI. Collectively, our results may explain the traditional usage of <i>C. terniflora</i> in inflammatory diseases and suggest the promising potential of EELCT as therapeutic candidate for ALI.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139409412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cow urine distillate (CUD) is a traditional Indian medicine used to treat various diseases, including bacterial infections. However, there is limited evidence to support its use as a medicine, and its safety and efficacy have not been thoroughly studied. In this study, we evaluated the antibacterial activity of CUD against five bacterial strains using in vitro and in silico approaches. In vitro experiments showed that CUD has significant antibacterial activity against all tested strains with a zone of inhibition (ZOI) ranging from 13 to 24 mm and minimum inhibitory concentration (MIC) values ranging from 12.5 to 50 µg/ml. The results indicated that the 15% concentration of CUD displayed the highest antibacterial activity against Staphylococcus aureus and Salmonella typhi. To further investigate the antibacterial mechanism of CUD, we performed in silico docking studies of the active compounds of CUD with bacterial proteins involved in protein synthesis. Our results showed that 2-hydroxycinnamic acid (ΔG = −6.9 kcal/mol) and ferulic acid (ΔG = −6.8 kcal/mol) exhibited the best docking scores with the targeted proteins (DNA gyrase, PDBID: 4KFG). The hydrogen bonding interaction with amino acids Val71 and Asp73 was found to be crucial for their antibacterial activity.
{"title":"In Silico and In Vitro Studies of Antibacterial Activity of Cow Urine Distillate (CUD)","authors":"LokRaj Pant, Shankar Thapa, Bibek Dahal, Ravindra Khadka, Mahalakshmi Suresha Biradar","doi":"10.1155/2024/1904763","DOIUrl":"https://doi.org/10.1155/2024/1904763","url":null,"abstract":"Cow urine distillate (CUD) is a traditional Indian medicine used to treat various diseases, including bacterial infections. However, there is limited evidence to support its use as a medicine, and its safety and efficacy have not been thoroughly studied. In this study, we evaluated the antibacterial activity of CUD against five bacterial strains using in vitro and in silico approaches. In vitro experiments showed that CUD has significant antibacterial activity against all tested strains with a zone of inhibition (ZOI) ranging from 13 to 24 mm and minimum inhibitory concentration (MIC) values ranging from 12.5 to 50 <i>µ</i>g/ml. The results indicated that the 15% concentration of CUD displayed the highest antibacterial activity against <i>Staphylococcus aureus</i> and <i>Salmonella typhi</i>. To further investigate the antibacterial mechanism of CUD, we performed in silico docking studies of the active compounds of CUD with bacterial proteins involved in protein synthesis. Our results showed that 2-hydroxycinnamic acid (Δ<i>G</i> = −6.9 kcal/mol) and ferulic acid (Δ<i>G</i> = −6.8 kcal/mol) exhibited the best docking scores with the targeted proteins (DNA gyrase, PDBID: 4KFG). The hydrogen bonding interaction with amino acids Val71 and Asp73 was found to be crucial for their antibacterial activity.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139398430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emanoeli da Rosa, Cheila Denise Ottonelli Stopiglia, Michel Mansur Machado, Augusto Cezar Dotta Filho, Ursula Paula Reno Soci, Andreas Sebastian Loureiro Mendez, Tiago Fernandes, Edilamar Menezes de Oliveira, Cleci Menezes Moreira
Natural compounds that have the potential to act as antimicrobials and antitumors are a constant search in the field of pharmacotherapy. Eragrostis plana NEES (Poaceae) is a grass with high allelopathic potential. Allelopathy is associated with compounds generated in the primary and secondary metabolism of the plant, which act to protect it from phytopathogens. Tabernaemontana catharinensis A DC (Apocynaceae), a tree in which its leaves and bark are used for the preparation of extracts and infusions that have anti-inflammatory and antinociceptive effects, is attributed to its phytochemical constitution. The objective of this study was to elucidate the phytochemical constitution, the antibacterial potential, the toxicity against immune system cells, hemolytic potential, and antitumor effect of methanolic extracts of E. plana and T. catharinensis. The phytochemical investigation was carried out using the UHPLC-QTOF MS equipment. The antibacterial activity was tested using the broth microdilution plate assay, against Gram-negative and Gram-positive strains, and cytotoxicity assays were performed on human peripheral blood mononuclear cells (PBMC) and in vitro hemolysis. Antitumor activity was performed against the colon cancer cell line (CT26). Results were expressed as mean and standard deviation and analyzed by ANOVA. was considered significant. More than 19 possible phytochemical constituents were identified for each plant, with emphasis on phenolic compounds (acids: vanillic, caffeic, and quinic) and alkaloids (alstovenine, rhyncophylline, amezepine, voacangine, and coronaridine). Both extracts showed antibacterial activity at concentrations below 500 µg/mL and were able to decrease the viability of CT26 at concentrations below 2000 µg/mL, without showing cytotoxic effect on PBMCs and in vitro hemolysis at the highest concentration tested. This is the first report of the activity of E. plana and T. catharinensis extracts against colon cancer cell line (CT26). Studies should be carried out to verify possible molecular targets involved in the antitum
药物疗法领域一直在寻找具有抗菌和抗肿瘤潜力的天然化合物。Eragrostis plana NEES（禾本科）是一种具有很高等位病理学潜力的草。等位异化作用与植物初级和次级代谢过程中产生的化合物有关，这些化合物能保护植物免受植物病原体的侵害。Tabernaemontana catharinensis A DC（天南星科）是一种树，其叶子和树皮可用于制备具有消炎和止痛效果的提取物和浸剂，这归功于其植物化学成分。本研究旨在阐明 E. plana 和 T. catharinensis 的甲醇提取物的植物化学成分、抗菌潜力、对免疫系统细胞的毒性、溶血潜力和抗肿瘤作用。植物化学研究是利用 UHPLC-QTOF MS 设备进行的。使用肉汤微量稀释平板法对革兰氏阴性和革兰氏阳性菌株进行了抗菌活性测试，并对人外周血单核细胞（PBMC）和体外溶血进行了细胞毒性测试。对结肠癌细胞株（CT26）进行了抗肿瘤活性检测。结果以平均值和标准偏差表示，并进行方差分析。每种植物都鉴定出了 19 种以上可能的植物化学成分，重点是酚类化合物（酸：香草酸、咖啡酸和奎宁酸）和生物碱（芒柄花碱、荷叶碱、阿米西平碱、瓦康碱和冕花碱）。这两种提取物在浓度低于 500 微克/毫升时显示出抗菌活性，在浓度低于 2000 微克/毫升时能够降低 CT26 的存活率，在测试的最高浓度下对 PBMC 没有细胞毒性作用，也没有体外溶血作用。这是首次报道 E. plana 和 T. catharinensis 提取物对结肠癌细胞系（CT26）的活性。应开展研究以验证参与体内抗肿瘤作用的可能分子靶点。
{"title":"Phytochemistry Profile, Antimicrobial and Antitumor Potential of the Methanolic Extract of Tabernaemontana catharinensis A DC and Eragrostis plana NEES","authors":"Emanoeli da Rosa, Cheila Denise Ottonelli Stopiglia, Michel Mansur Machado, Augusto Cezar Dotta Filho, Ursula Paula Reno Soci, Andreas Sebastian Loureiro Mendez, Tiago Fernandes, Edilamar Menezes de Oliveira, Cleci Menezes Moreira","doi":"10.1155/2024/5513141","DOIUrl":"https://doi.org/10.1155/2024/5513141","url":null,"abstract":"Natural compounds that have the potential to act as antimicrobials and antitumors are a constant search in the field of pharmacotherapy. <i>Eragrostis plana</i> NEES (Poaceae) is a grass with high allelopathic potential. Allelopathy is associated with compounds generated in the primary and secondary metabolism of the plant, which act to protect it from phytopathogens. <i>Tabernaemontana catharinensis</i> A DC (Apocynaceae), a tree in which its leaves and bark are used for the preparation of extracts and infusions that have anti-inflammatory and antinociceptive effects, is attributed to its phytochemical constitution. The objective of this study was to elucidate the phytochemical constitution, the antibacterial potential, the toxicity against immune system cells, hemolytic potential, and antitumor effect of methanolic extracts of <i>E. plana</i> and <i>T. catharinensis.</i> The phytochemical investigation was carried out using the UHPLC-QTOF MS equipment. The antibacterial activity was tested using the broth microdilution plate assay, against Gram-negative and Gram-positive strains, and cytotoxicity assays were performed on human peripheral blood mononuclear cells (PBMC) and <i>in vitro</i> hemolysis. Antitumor activity was performed against the colon cancer cell line (CT26). Results were expressed as mean and standard deviation and analyzed by ANOVA. <span><svg height=\"11.7782pt\" style=\"vertical-align:-3.42938pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 18.973 11.7782\" width=\"18.973pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,11.342,0)\"></path></g></svg><span></span><svg height=\"11.7782pt\" style=\"vertical-align:-3.42938pt\" version=\"1.1\" viewbox=\"22.555183800000002 -8.34882 21.921 11.7782\" width=\"21.921pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,22.605,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,28.845,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,31.809,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,38.049,0)\"></path></g></svg></span> was considered significant. More than 19 possible phytochemical constituents were identified for each plant, with emphasis on phenolic compounds (acids: vanillic, caffeic, and quinic) and alkaloids (alstovenine, rhyncophylline, amezepine, voacangine, and coronaridine). Both extracts showed antibacterial activity at concentrations below 500 <i>µ</i>g/mL and were able to decrease the viability of CT26 at concentrations below 2000 <i>µ</i>g/mL, without showing cytotoxic effect on PBMCs and <i>in vitro</i> hemolysis at the highest concentration tested. This is the first report of the activity of <i>E. plana</i> and <i>T. catharinensis</i> extracts against colon cancer cell line (CT26). Studies should be carried out to verify possible molecular targets involved in the antitum","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139083019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. The ayurvedic plant Withania somnifera, a member of the Solanaceae family, has been used as a remedy for diverse health problems, including cancer. Objectives. The objective of this investigation was to conduct a comparative analysis of the in vitro cytotoxic properties of methanolic extracts derived from the leaf, stem, and root of W. somnifera on HepG2 and L929 cell lines. Methods. Methanolic extracts were obtained using the Soxhlet extraction method. To assess the in vitro anticancer action on the HepG2 and L929 cell lines, an MTT assay was performed. Changes in cell morphology were observed using an inverted microscope. Results. The MTT assay results indicated that the leaf, stem, and root methanolic extracts of W. somnifera showed significantly higher in vitro cytotoxicity in HepG2 cells, with IC50 values of 43.06 ± 0.615, 45.60 ± 0.3, and 314.4 ± 0.795 μg/mL than in L929 cell lines with 78.77 ± 0.795, 90.55 ± 0.800, and 361.70 ± 0.795 μg/mL, respectively. The leaf methanolic extract was the most effective, followed by the stem methanolic extract in the HepG2 cell line. Conclusion. The results of our study have confirmed that the methanolic extracts of both the leaf and stem of W. somnifera exhibit significant in vitro cytotoxicity in HepG2 cell lines, while displaying no significant cytotoxicity in the L929 cell line. Furthermore, the data obtained from the MTT assay indicate that the leaf methanolic extract possesses a more potent cytotoxic activity than the stem methanolic extract with respect to the HepG2 cell line. Further studies on the identification and isolation of bioactive metabolites are required to explore the mechanisms underlying their in vitro cytotoxicity.
{"title":"In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line","authors":"Lali Lingfa, Aravinda Tirumala, Srinivas Ankanagari","doi":"10.1155/2023/8832166","DOIUrl":"https://doi.org/10.1155/2023/8832166","url":null,"abstract":"<i>Background</i>. The ayurvedic plant <i>Withania somnifera</i>, a member of the Solanaceae family, has been used as a remedy for diverse health problems, including cancer. <i>Objectives</i>. The objective of this investigation was to conduct a comparative analysis of the <i>in vitro</i> cytotoxic properties of methanolic extracts derived from the leaf, stem, and root of <i>W. somnifera</i> on HepG2 and L929 cell lines. <i>Methods</i>. Methanolic extracts were obtained using the Soxhlet extraction method. To assess the <i>in vitro</i> anticancer action on the HepG2 and L929 cell lines, an MTT assay was performed. Changes in cell morphology were observed using an inverted microscope. <i>Results</i>. The MTT assay results indicated that the leaf, stem, and root methanolic extracts of <i>W. somnifera</i> showed significantly higher <i>in vitro</i> cytotoxicity in HepG2 cells, with IC<sub>50</sub> values of 43.06 ± 0.615, 45.60 ± 0.3, and 314.4 ± 0.795 <i>μ</i>g/mL than in L929 cell lines with 78.77 ± 0.795, 90.55 ± 0.800, and 361.70 ± 0.795 <i>μ</i>g/mL, respectively. The leaf methanolic extract was the most effective, followed by the stem methanolic extract in the HepG2 cell line. <i>Conclusion</i>. The results of our study have confirmed that the methanolic extracts of both the leaf and stem of <i>W. somnifera</i> exhibit significant <i>in vitro</i> cytotoxicity in HepG2 cell lines, while displaying no significant cytotoxicity in the L929 cell line. Furthermore, the data obtained from the MTT assay indicate that the leaf methanolic extract possesses a more potent cytotoxic activity than the stem methanolic extract with respect to the HepG2 cell line. Further studies on the identification and isolation of bioactive metabolites are required to explore the mechanisms underlying their <i>in vitro</i> cytotoxicity.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Ansong, Desmond Nkrumah, Reinhard Isaac Nketia, Samuel Oppong Bekoe, Abena Amponsaa Brobbey, Evelyn Asante-Kwatia, Gustav Komlaga
Sida cordifolia has been used to treat malaria in Ghana albeit without scientific evidence of antimalarial activity and safety. This work aimed to assess the antimalarial properties and acute toxicity of the aqueous leaf extract of S. cordifolia in murine models. Aqueous extract of the plant was analysed for both suppressive and curative antimalarial properties in chloroquine-sensitive ANKA strains of rodent Plasmodium berghei-infected mice. Acute toxicity evaluation was performed in rats according to the OECD 425 guidelines. The extract displayed antiplasmodial activity in vivo with ED50 of 117.49 ± 15.22 mg/kg and 144.84 ± 18.17 mg/kg in suppressive and curative studies, respectively. The highest % parasitaemia suppression exerted was 76.90 ± 0.64% and 61.50 ± 0.97%, respectively, in the suppressive and curative studies. Survival of infected mice treated with the extract was significantly prolonged. This was dependent on the dose of the extract but imperfectly related to the % parasitaemia suppression. Related antimalarial parameters including percentage hematocrit, changes in body weight, and temperature of experimental mice indicated alleviation of malarial symptoms of treated animals. The extract did not show toxicity in rats. Sida cordifolia L. has antimalarial properties, and was safe. It suppressed parasitaemia in both suppressive and curative studies, was not toxic to animals and prolonged the life of infected animals under treatment. This, therefore, justifies the traditional use of S. cordifolia for the treatment of malaria in Ghana.
{"title":"Antimalarial Properties of Sida cordifolia L. Leaf Extract in Mice: Survivability Depends Less on Parasitaemia Suppression","authors":"Samuel Ansong, Desmond Nkrumah, Reinhard Isaac Nketia, Samuel Oppong Bekoe, Abena Amponsaa Brobbey, Evelyn Asante-Kwatia, Gustav Komlaga","doi":"10.1155/2023/5560711","DOIUrl":"https://doi.org/10.1155/2023/5560711","url":null,"abstract":"<i>Sida cordifolia</i> has been used to treat malaria in Ghana albeit without scientific evidence of antimalarial activity and safety. This work aimed to assess the antimalarial properties and acute toxicity of the aqueous leaf extract of <i>S. cordifolia</i> in murine models. Aqueous extract of the plant was analysed for both suppressive and curative antimalarial properties in chloroquine-sensitive ANKA strains of rodent <i>Plasmodium berghei</i>-infected mice. Acute toxicity evaluation was performed in rats according to the OECD 425 guidelines. The extract displayed antiplasmodial activity <i>in vivo</i> with ED<sub>50</sub> of 117.49 ± 15.22 mg/kg and 144.84 ± 18.17 mg/kg in suppressive and curative studies, respectively. The highest % parasitaemia suppression exerted was 76.90 ± 0.64% and 61.50 ± 0.97%, respectively, in the suppressive and curative studies. Survival of infected mice treated with the extract was significantly prolonged. This was dependent on the dose of the extract but imperfectly related to the % parasitaemia suppression. Related antimalarial parameters including percentage hematocrit, changes in body weight, and temperature of experimental mice indicated alleviation of malarial symptoms of treated animals. The extract did not show toxicity in rats. <i>Sida cordifolia</i> L. has antimalarial properties, and was safe. It suppressed parasitaemia in both suppressive and curative studies, was not toxic to animals and prolonged the life of infected animals under treatment. This, therefore, justifies the traditional use of <i>S. cordifolia</i> for the treatment of malaria in Ghana.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139022460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katiana Simões Lopes, A. A. M. Marques, Karyne Garcia Tafarelo Moreno, A. C. dos Santos, R. I. Souza, E. Lourenço, Rodrigo Juliano Oliveira, R. Gomes, F. Lívero, A. Gasparotto Júnior
Solidago microglossa DC. (Asteraceae), “arnica brasileira,” is a Brazilian species popularly used to treat hypertension or renal ailments. This study investigated the cardioprotective effects of standardized S. microglossa extract (EESM) in nicotine-treated spontaneously hypertensive rats (SHRs). Moreover, the molecular mechanisms involved in the cardiovascular effects were also investigated. The acute toxicity was evaluated in female Wistar rats. Afterwards, six-month-old male spontaneously hypertensive rats received the EESM (14, 28, and 56 mg/kg), hydrochlorothiazide (25 mg/kg), and vehicle (filtered water; 0.1 mL/100 g) once daily for 28 days. All treatments were associated with 1.8 mg/kg of nicotine. At the end of the experimental period, the renal function, electrocardiographic profile, blood pressure, ventricular function, biochemical parameter, and mesenteric vascular bed reactivity were evaluated. Relative organ weights and cardiac morphometry were also investigated. Nicotine treatment in 6-month-old SHRs induced a significant reduction in renal function, with reduced urinary volume and lower renal elimination of sodium and creatinine. In addition, serum markers of the redox state and blood pressure levels remained significantly elevated, contributing to changes in vascular reactivity and left ventricular hypertrophy associated with reduced ventricular function. After 28 days of treatment, we found that the highest dose of EESM could mitigate all renal and cardiovascular changes developed by the nicotine-treated hypertensive rats. This study presented EESM as a possible cardioprotective drug that prevents cardiovascular dysfunctions in nicotine-treated hypertensive rats. Our data suggest EESM as a potential adjuvant therapy when cardioprotective effects are required.
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Pub Date : 2023-12-13eCollection Date: 2023-01-01DOI: 10.1155/2023/9826513
Evidence-Based Complementary And Alternative Medicine
[This retracts the article DOI: 10.1155/2021/1426753.].
[本文撤回文章 DOI：10.1155/2021/1426753]。
{"title":"Retracted: Clinical Study of Stellate Ganglion Block Combined with General Anesthesia on Hemodynamics, Cognitive Function, and Gastrointestinal Function in Elderly Patients Undergoing Partial Hepatectomy.","authors":"Evidence-Based Complementary And Alternative Medicine","doi":"10.1155/2023/9826513","DOIUrl":"https://doi.org/10.1155/2023/9826513","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2021/1426753.].</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"2023 ","pages":"9826513"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138836034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-13eCollection Date: 2023-01-01DOI: 10.1155/2023/9848149
Evidence-Based Complementary And Alternative Medicine
[This retracts the article DOI: 10.1155/2023/1744625.].
[此文撤稿，DOI: 10.1155/2023/1744625.]。
{"title":"Retracted: Clinical Value of Serum SIRT1 Combined with Uterine Hemodynamics in Predicting Disease Severity and Fetal Growth Restriction in Preeclampsia.","authors":"Evidence-Based Complementary And Alternative Medicine","doi":"10.1155/2023/9848149","DOIUrl":"https://doi.org/10.1155/2023/9848149","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2023/1744625.].</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"2023 ","pages":"9848149"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138836035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-13eCollection Date: 2023-01-01DOI: 10.1155/2023/9804916
Evidence-Based Complementary And Alternative Medicine
[This retracts the article DOI: 10.1155/2022/6507865.].
[本文撤消了文章 DOI：10.1155/2022/6507865]。
{"title":"Retracted: Value of MSCT plus MRI in the Detection of Colon Cancer.","authors":"Evidence-Based Complementary And Alternative Medicine","doi":"10.1155/2023/9804916","DOIUrl":"https://doi.org/10.1155/2023/9804916","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/6507865.].</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"2023 ","pages":"9804916"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138836144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-13eCollection Date: 2023-01-01DOI: 10.1155/2023/9898674
Evidence-Based Complementary And Alternative Medicine
[This retracts the article DOI: 10.1155/2021/4288739.].
[本文撤回了文章 DOI：10.1155/2021/4288739]。
{"title":"Retracted: Changes of Intestinal Flora and Lymphocyte Subsets in Patients with Chronic Renal Failure.","authors":"Evidence-Based Complementary And Alternative Medicine","doi":"10.1155/2023/9898674","DOIUrl":"https://doi.org/10.1155/2023/9898674","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2021/4288739.].</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"2023 ","pages":"9898674"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138829031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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