Pub Date : 2025-02-25DOI: 10.12116/j.issn.1004-5619.2024.440607
Cheng-Tao Li, Bin Cong
The development of forensic medicine is crucial to the national welfare, people's livelihood, social stability, and the construction of a Safe China. It plays an indispensable role in the comprehensive promotion of the rule of law. The establishment of forensic medicine as a first-level discipline, along with the transformation brought by the fifth research paradigm, makes the construction of forensic medicine stand at a new historical starting point. This paper reviews the current status and challenges of forensic medicine development, outlines the characteristics of the fifth research paradigm and its impact on the construction of forensic medicine, and proposes strategies for addressing these challenges.
{"title":"Thoughts on the Development of Forensic Medicine Discipline Standing at a New Historical Starting Point.","authors":"Cheng-Tao Li, Bin Cong","doi":"10.12116/j.issn.1004-5619.2024.440607","DOIUrl":"10.12116/j.issn.1004-5619.2024.440607","url":null,"abstract":"<p><p>The development of forensic medicine is crucial to the national welfare, people's livelihood, social stability, and the construction of a Safe China. It plays an indispensable role in the comprehensive promotion of the rule of law. The establishment of forensic medicine as a first-level discipline, along with the transformation brought by the fifth research paradigm, makes the construction of forensic medicine stand at a new historical starting point. This paper reviews the current status and challenges of forensic medicine development, outlines the characteristics of the fifth research paradigm and its impact on the construction of forensic medicine, and proposes strategies for addressing these challenges.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"41 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-25DOI: 10.12116/j.issn.1004-5619.2024.540101
Hong-Yu Sun, Hu Zhao
The forensic medicine discipline of Sun Yat-sen University is one of the important origins of modern and contemporary forensic medicine education in China, and it is a microcosm of the development of forensic medicine education, research and identification practice in the People's Republic of China. This paper introduces the historical evolution, current situation, opportunities and challenges faced by the forensic medicine discipline of Sun Yat-sen University. Based on the historical new starting point of forensic medicine becoming a first-level discipline, it analyzes the construction goals and measures of the forensic medicine discipline of Sun Yat-sen University in the new era, aiming to reach consensus and promote the high-quality development of forensic medicine.
{"title":"Seventy Years of Passing on the Torch, Striving Forward in the New Era - Thoughts on the Construction of Forensic Medicine at Sun Yat-sen University.","authors":"Hong-Yu Sun, Hu Zhao","doi":"10.12116/j.issn.1004-5619.2024.540101","DOIUrl":"10.12116/j.issn.1004-5619.2024.540101","url":null,"abstract":"<p><p>The forensic medicine discipline of Sun Yat-sen University is one of the important origins of modern and contemporary forensic medicine education in China, and it is a microcosm of the development of forensic medicine education, research and identification practice in the People's Republic of China. This paper introduces the historical evolution, current situation, opportunities and challenges faced by the forensic medicine discipline of Sun Yat-sen University. Based on the historical new starting point of forensic medicine becoming a first-level discipline, it analyzes the construction goals and measures of the forensic medicine discipline of Sun Yat-sen University in the new era, aiming to reach consensus and promote the high-quality development of forensic medicine.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"41 1","pages":"20-24"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: To establish a highly sensitive and specific method for detecting human DNA based on real time quantitative PCR (qPCR) technique for the rapid detection of potential DNA contamination sources in DNA laboratories.
Methods: Primers and probes were designed with Primer ExpressTM software using the reference sequence of human 18S rRNA gene as a template, and the optimal prime-probe combination was screened by matrix method. The PCR products of the target sequence of human 18S rRNA gene were used to construct the plasmid, and a plasmid standard was used to draw the standard curve of the qPCR system. According to the Minimum Information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines, the specificity, sensitivity, repeatability and application effect of the qPCR system were evaluated.
Results: The sensitivity of the qPCR system established in this study was 5.3×10-5 ng/μL, which showed good specificity for human DNA samples. The correlation coefficient of the qPCR system was -0.999, and amplification efficiency was 100%. Both the intra-batch and inter-batch variation coefficients were less than 2%.
Conclusions: The established human DNA detection method based on qPCR technique has good specificity, high sensitivity, and robust stability. It can be used for rapid detection of DNA contamination and daily monitoring of the accumulated human DNA in the laboratory environment.
目的：建立一种基于实时定量PCR （real - time quantitative PCR, qPCR）技术的高灵敏度、高特异性的人DNA检测方法，用于DNA实验室中潜在DNA污染源的快速检测。方法：以人18S rRNA基因参考序列为模板，利用Primer ExpressTM软件设计引物和探针，采用矩阵法筛选最佳引物和探针组合。利用人18S rRNA基因目的序列的PCR产物构建质粒，用质粒标准品绘制qPCR体系的标准曲线。根据MIQE （Minimum Information for Publication of Quantitative Real-time PCR Experiments）指南，对qPCR系统的特异性、敏感性、重复性和应用效果进行评价。结果：本研究建立的qPCR系统灵敏度为5.3×10-5 ng/μL，对人DNA样品具有良好的特异性。qPCR体系的相关系数为-0.999，扩增效率为100%。批内和批间变异系数均小于2%。结论：建立的基于qPCR技术的人DNA检测方法特异性好、灵敏度高、鲁棒稳定性好。它可用于DNA污染的快速检测和实验室环境中积累的人类DNA的日常监测。
{"title":"Establishment and Application of TaqMan qPCR Detection Method for Human DNA Contamination in DNA Laboratory.","authors":"Gao-Fang Shen, Yong-Song Zhou, Jian-Qiu Zhang, Shi-You Ji, Ying-Feng Wu, Hao Shang, Bo-Feng Zhu","doi":"10.12116/j.issn.1004-5619.2023.531004","DOIUrl":"10.12116/j.issn.1004-5619.2023.531004","url":null,"abstract":"<p><strong>Objectives: </strong>To establish a highly sensitive and specific method for detecting human DNA based on real time quantitative PCR (qPCR) technique for the rapid detection of potential DNA contamination sources in DNA laboratories.</p><p><strong>Methods: </strong>Primers and probes were designed with Primer Express<sup>TM</sup> software using the reference sequence of human 18S rRNA gene as a template, and the optimal prime-probe combination was screened by matrix method. The PCR products of the target sequence of human 18S rRNA gene were used to construct the plasmid, and a plasmid standard was used to draw the standard curve of the qPCR system. According to the Minimum Information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines, the specificity, sensitivity, repeatability and application effect of the qPCR system were evaluated.</p><p><strong>Results: </strong>The sensitivity of the qPCR system established in this study was 5.3×10<sup>-5</sup> ng/μL, which showed good specificity for human DNA samples. The correlation coefficient of the qPCR system was -0.999, and amplification efficiency was 100%. Both the intra-batch and inter-batch variation coefficients were less than 2%.</p><p><strong>Conclusions: </strong>The established human DNA detection method based on qPCR technique has good specificity, high sensitivity, and robust stability. It can be used for rapid detection of DNA contamination and daily monitoring of the accumulated human DNA in the laboratory environment.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"41 1","pages":"66-73"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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