Pub Date : 2025-06-01DOI: 10.17113/ftb.63.02.25.8904
Ze Sen Tan, M Devendran Manogaran, Rashid Shamsuddin, Mohd Hakimi, Lee Wen Looi, Kai Tong Woo, Chin Seng Liew, Lailatul Qomariyah
Research background: The rapid growth of the Malaysian population has led to an increase in kitchen waste, especially inedible organic kitchen waste, which is generally disposed of in landfills and pollutes the environment. Apart from this, the increasing demand for chicken products in Malaysia has led to a significant increase in chicken manure production. As anaerobic digestion continues to be explored, there are concerns about the utilization of the digestate from chicken manure. Therefore, this study addresses the challenge of treating kitchen waste and chicken manure digestate in Malaysia by investigating the effectiveness of composting and vermicomposting methods through comparative analysis. By integrating kitchen waste, particularly spent coffee grounds, bone waste and used kitchen towels, this study aims to improve the imbalanced physicochemical properties of digestate from chicken manure.
Experimental approach: Before composting, the kitchen waste and chicken manure digestate were characterised to determine the initial physicochemical properties. Four composting setups comprising the substances were established to study the physical appearance, temperature and pH profile, the increase in nitrogen, phosphorus and potassium content, and the mass reduction of the final compost after 50 days of composting.
Results and conclusions: The vermicompost with kitchen waste additives showed a significant nutrient improvement with an NPK mass ratio of 1:3.57:6.58 and a lower moisture mass fraction of 48.92 %, which requires the shortest maturation time (20 days) and the highest mass reduction (55.11 %).
Novelty and scientific contribution: The novelty of this research is the valorisation of organic kitchen waste and chicken manure digestate as biofertiliser. The end result is achieved by promoting a sustainable alternative to exploit kitchen waste instead of the traditional approach of landfilling waste. At the same time the problem of digestate is addressed, particularly its unbalanced physicochemical properties, especially its macronutrients, pH and moisture content. In contrast to previous studies, this work investigates the effectiveness of both conventional composting and vermicomposting with the incorporation of organic kitchen waste, namely spent coffee grounds, bone meal and used kitchen towels, to improve the physicochemical properties of digestate.
{"title":"Comparative Study on Composting and Vermicomposting to Improve Physicochemical Properties of Digestate with the Addition of Kitchen Waste<sup>§</sup>.","authors":"Ze Sen Tan, M Devendran Manogaran, Rashid Shamsuddin, Mohd Hakimi, Lee Wen Looi, Kai Tong Woo, Chin Seng Liew, Lailatul Qomariyah","doi":"10.17113/ftb.63.02.25.8904","DOIUrl":"10.17113/ftb.63.02.25.8904","url":null,"abstract":"<p><strong>Research background: </strong>The rapid growth of the Malaysian population has led to an increase in kitchen waste, especially inedible organic kitchen waste, which is generally disposed of in landfills and pollutes the environment. Apart from this, the increasing demand for chicken products in Malaysia has led to a significant increase in chicken manure production. As anaerobic digestion continues to be explored, there are concerns about the utilization of the digestate from chicken manure. Therefore, this study addresses the challenge of treating kitchen waste and chicken manure digestate in Malaysia by investigating the effectiveness of composting and vermicomposting methods through comparative analysis. By integrating kitchen waste, particularly spent coffee grounds, bone waste and used kitchen towels, this study aims to improve the imbalanced physicochemical properties of digestate from chicken manure.</p><p><strong>Experimental approach: </strong>Before composting, the kitchen waste and chicken manure digestate were characterised to determine the initial physicochemical properties. Four composting setups comprising the substances were established to study the physical appearance, temperature and pH profile, the increase in nitrogen, phosphorus and potassium content, and the mass reduction of the final compost after 50 days of composting.</p><p><strong>Results and conclusions: </strong>The vermicompost with kitchen waste additives showed a significant nutrient improvement with an NPK mass ratio of 1:3.57:6.58 and a lower moisture mass fraction of 48.92 %, which requires the shortest maturation time (20 days) and the highest mass reduction (55.11 %).</p><p><strong>Novelty and scientific contribution: </strong>The novelty of this research is the valorisation of organic kitchen waste and chicken manure digestate as biofertiliser. The end result is achieved by promoting a sustainable alternative to exploit kitchen waste instead of the traditional approach of landfilling waste. At the same time the problem of digestate is addressed, particularly its unbalanced physicochemical properties, especially its macronutrients, pH and moisture content. In contrast to previous studies, this work investigates the effectiveness of both conventional composting and vermicomposting with the incorporation of organic kitchen waste, namely spent coffee grounds, bone meal and used kitchen towels, to improve the physicochemical properties of digestate.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"149-158"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.17113/ftb.63.02.25.8560
Nita Kaushik, Aradhita Barmanray
<p><strong>Research background: </strong><i>Nigella sativa</i> L., commonly known as black cumin, is a medicinal plant renowned for its rich bioactive composition and health-promoting properties. Among its key compounds, thymoquinone has gained significant attention in nutraceutical and pharmaceutical research for its potential to prevent and manage chronic inflammatory conditions and immune dysfunctions. With growing global interest in natural health solutions, the aim of this study is to optimise ultrasound-assisted extraction (UAE) conditions to maximise thymoquinone yield from the extract of black cumin (<i>Nigella sativa</i> L.) seeds and characterise the bioactive compounds. By using UAE and advanced analytical techniques, the research contributes to the development of sustainable extracts rich in bioactive compounds with applications in medicine and nutrition.</p><p><strong>Experimental approach: </strong>In this study, ultrasound-assisted extraction method was used with response surface methodology (RSM) software to extract the bioactive compounds, including total phenolic content (TPC) and compounds that can bind free DPPH radical. To increase the extraction efficiency of bioactive compounds, the following parameters were examined: the ratio of the mass of seed powder to the volume of solvent of 50-100 %, extraction temperature of 30 °C, amplitude of 30-60 % and extraction time of 30-60 min. Black cumin seed extracts were characterised using scanning electron microscopy (SEM), while gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify thymoquinone. Additionally, Fourier transform infrared (FTIR) spectroscopy confirmed the presence of thymoquinone and several functional groups, including amines, alkanes, acids, esters, alkyls and alkenes.</p><p><strong>Results and conclusions: </strong>Ultrasonic extraction using methanol as a solvent resulted in a higher yield of thymoquinone (28.62 %), identified using GC-MS analysis. The presence of thymoquinone was further confirmed by the functional groups detected in FTIR analysis. Under the specified extraction conditions, total phenolic content (TPC, expressed as gallic acid equivalents), yield (in %) and DPPH radical scavenging activity increased by approx. 271.03 mg/g, and 4.5 and 83.06 %, respectively. In addition to thymoquinone, thymohydroquinone was also identified based on its molecular mass, retention time and peak values. Thymoquinone, a natural and potent phytochemical, offers a range of therapeutic properties, including immune-enhancing potential.</p><p><strong>Novelty and scientific contribution: </strong>Thymoquinone is a bioactive compound found in black cumin seeds, known for its potent antioxidant and immunity boosting properties. This research was conducted achieve the best possible extraction conditions for bioactive substances. Additionally, the results support the potential of thymoquinone as a therapeutic agent to treat various health conditions. T
{"title":"Optimisation of Ultrasound-Assisted Extraction Conditions Using Response Surface Methodology and Identification of Thymoquinone from Black Cumin (<i>Nigella sativa</i> L.) Seed Extract<sup>§</sup>.","authors":"Nita Kaushik, Aradhita Barmanray","doi":"10.17113/ftb.63.02.25.8560","DOIUrl":"10.17113/ftb.63.02.25.8560","url":null,"abstract":"<p><strong>Research background: </strong><i>Nigella sativa</i> L., commonly known as black cumin, is a medicinal plant renowned for its rich bioactive composition and health-promoting properties. Among its key compounds, thymoquinone has gained significant attention in nutraceutical and pharmaceutical research for its potential to prevent and manage chronic inflammatory conditions and immune dysfunctions. With growing global interest in natural health solutions, the aim of this study is to optimise ultrasound-assisted extraction (UAE) conditions to maximise thymoquinone yield from the extract of black cumin (<i>Nigella sativa</i> L.) seeds and characterise the bioactive compounds. By using UAE and advanced analytical techniques, the research contributes to the development of sustainable extracts rich in bioactive compounds with applications in medicine and nutrition.</p><p><strong>Experimental approach: </strong>In this study, ultrasound-assisted extraction method was used with response surface methodology (RSM) software to extract the bioactive compounds, including total phenolic content (TPC) and compounds that can bind free DPPH radical. To increase the extraction efficiency of bioactive compounds, the following parameters were examined: the ratio of the mass of seed powder to the volume of solvent of 50-100 %, extraction temperature of 30 °C, amplitude of 30-60 % and extraction time of 30-60 min. Black cumin seed extracts were characterised using scanning electron microscopy (SEM), while gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify thymoquinone. Additionally, Fourier transform infrared (FTIR) spectroscopy confirmed the presence of thymoquinone and several functional groups, including amines, alkanes, acids, esters, alkyls and alkenes.</p><p><strong>Results and conclusions: </strong>Ultrasonic extraction using methanol as a solvent resulted in a higher yield of thymoquinone (28.62 %), identified using GC-MS analysis. The presence of thymoquinone was further confirmed by the functional groups detected in FTIR analysis. Under the specified extraction conditions, total phenolic content (TPC, expressed as gallic acid equivalents), yield (in %) and DPPH radical scavenging activity increased by approx. 271.03 mg/g, and 4.5 and 83.06 %, respectively. In addition to thymoquinone, thymohydroquinone was also identified based on its molecular mass, retention time and peak values. Thymoquinone, a natural and potent phytochemical, offers a range of therapeutic properties, including immune-enhancing potential.</p><p><strong>Novelty and scientific contribution: </strong>Thymoquinone is a bioactive compound found in black cumin seeds, known for its potent antioxidant and immunity boosting properties. This research was conducted achieve the best possible extraction conditions for bioactive substances. Additionally, the results support the potential of thymoquinone as a therapeutic agent to treat various health conditions. T","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"262-273"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Waste management in the food manufacturing sector has become one of the most challenging aspects globally owing to the generation of enormous quantities of by-products, such as peels, seeds and undesirable flesh at various stages of the processing chain. However, these plant by-products are rich in important compounds particularly polyphenols and bioactive substances that significantly affect human health and can be utilised in numerous sectors as new, low-cost and economical raw ingredients. The aim of this review paper is to discuss various methods of valorising food waste, concentrating on upcycling, aquafaba, coffee silver skin, propolis, wine lees and avocado waste. Food waste is a substantial global issue, with the potential to affect food security, environment and economy. Upcycling is highlighted as a means to tackle food waste by repurposing high-value by-products such as fruit and vegetable residues. Aquafaba, a vegan alternative to egg white, is produced from chickpeas and has various culinary applications. Coffee silver skin, a by-product of coffee production, contains bioactive compounds that can be extracted and used in functional foods. Propolis, a resinous substance collected by bees, is rich in bioactive compounds with health benefits. Wine lees, a by-product of winemaking, can be processed to extract phenolic compounds and produce value-added products. Avocado waste valorisation focuses on converting avocado by-products into valuable products for various industries. The sustainable valorisation of food waste offers numerous benefits, such as reducing waste output, generating revenue and promoting resource efficiency. Collaboration between stakeholders is essential to advance research and implement sustainable management practices for food waste valorisation to achieve the Sustainable Development Goals (SDGs). Challenges such as scaling-up, regulatory frameworks, logistics, food safety and environmental impact must be addressed to effectively valorise food waste.
{"title":"Turning Waste into Taste: Effective Upcycling of By-Products for Innovative Food Solutions<sup>§</sup>.","authors":"Swapna Sree Meduri, Sujatha Mudawath, Prabhakar Butti, Soujanya Kanneboina, Sucharita Devi Tattepalli, Supraja Thoomati, Neela Rani Rathod, Aparna Kuna, Krishna Lavuri, Srinivasa Chary Darshanoju, Kanmani Kalaivanan","doi":"10.17113/ftb.63.02.25.8962","DOIUrl":"10.17113/ftb.63.02.25.8962","url":null,"abstract":"<p><p>Waste management in the food manufacturing sector has become one of the most challenging aspects globally owing to the generation of enormous quantities of by-products, such as peels, seeds and undesirable flesh at various stages of the processing chain. However, these plant by-products are rich in important compounds particularly polyphenols and bioactive substances that significantly affect human health and can be utilised in numerous sectors as new, low-cost and economical raw ingredients. The aim of this review paper is to discuss various methods of valorising food waste, concentrating on upcycling, aquafaba, coffee silver skin, propolis, wine lees and avocado waste. Food waste is a substantial global issue, with the potential to affect food security, environment and economy. Upcycling is highlighted as a means to tackle food waste by repurposing high-value by-products such as fruit and vegetable residues. Aquafaba, a vegan alternative to egg white, is produced from chickpeas and has various culinary applications. Coffee silver skin, a by-product of coffee production, contains bioactive compounds that can be extracted and used in functional foods. Propolis, a resinous substance collected by bees, is rich in bioactive compounds with health benefits. Wine lees, a by-product of winemaking, can be processed to extract phenolic compounds and produce value-added products. Avocado waste valorisation focuses on converting avocado by-products into valuable products for various industries. The sustainable valorisation of food waste offers numerous benefits, such as reducing waste output, generating revenue and promoting resource efficiency. Collaboration between stakeholders is essential to advance research and implement sustainable management practices for food waste valorisation to achieve the Sustainable Development Goals (SDGs). Challenges such as scaling-up, regulatory frameworks, logistics, food safety and environmental impact must be addressed to effectively valorise food waste.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"190-205"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.17113/ftb.63.02.25.8808
Dae-Seok Lee, Eun Jin Cho, Seryung Kim, Dien Thanh Nguyen, Hyeun-Jong Bae
Research background: Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:SucE12-ΔldhA, a strain overexpressing the succinic acid transporter (SucE).
Experimental approach: The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass - bamboo, oak, poplar, pine and spent coffee grounds - were analysed to determine the most favourable bioresource for succinic acid production.
Results and conclusions: Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38-24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness.
Novelty and scientific contribution: This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability.
{"title":"Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered <i>Corynebacterium glutamicum</i> <sup>§</sup>.","authors":"Dae-Seok Lee, Eun Jin Cho, Seryung Kim, Dien Thanh Nguyen, Hyeun-Jong Bae","doi":"10.17113/ftb.63.02.25.8808","DOIUrl":"10.17113/ftb.63.02.25.8808","url":null,"abstract":"<p><strong>Research background: </strong>Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:<i>SucE</i>12-<i>ΔldhA</i>, a strain overexpressing the succinic acid transporter (<i>SucE</i>).</p><p><strong>Experimental approach: </strong>The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass - bamboo, oak, poplar, pine and spent coffee grounds - were analysed to determine the most favourable bioresource for succinic acid production.</p><p><strong>Results and conclusions: </strong>Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38-24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness.</p><p><strong>Novelty and scientific contribution: </strong>This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"134-148"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.17113/ftb.63.02.25.8792
Joanna Lisa Clifford, Yi Jing Chan, Mohd Amran Bin Mohd Yusof, Timm Joyce Tiong, Siew Shee Lim, Chai Siah Lee, Woei-Yenn Tong
Research background: Biogas production from palm oil mill effluent (POME) is inherently unstable due to variations in feedstock composition and operating conditions. These fluctuations can lead to inconsistent biogas quality, variable methane content and fluctuating hydrogen sulphide (H2S) concentration. This poses significant challenges for bioscrubbers in removing H2S to meet quality standards for gas engines used for electricity generation. This research aims to address these challenges by integrating simulation models with a computer programme and artificial neural network (ANN) to predict the performance of a bioscrubber at a POME treatment plant in Johor, Malaysia.
Experimental approach: First, the process flowsheet model was simulated using a computer programme. The H2S removal was then predicted using a machine learning algorithm, specifically ANN, based on two years of historical data obtained from the biogas plant. A detailed techno-economic analysis was also carried out to determine the economic feasibility of the process.
Results and conclusions: The simulation results showed a biogas yield of 26.12 Nm3 per m3 POME, which is in line with industry data with less than 1 % deviation. The ANN model achieved a high coefficient of determination (R2) of 0.9 and a low mean squared error (MSE), with the bioscrubber reaching an H2S removal efficiency of approx. 96 %. The techno-economic analysis showed that the process is feasible with a net present value (NPV) of $131 000 and a payback period of 7 years.
Novelty and scientific contribution: The integration of ANN and the computer programme provides a robust framework for predicting bioscrubber performance and ensuring stable bioscrubber operation due to their complementary strengths. ANN accurately predicts H2S removal based on daily recorded data, while the computer programme estimates parameters that are not monitored daily, such as chemical oxygen demand (COD), biological oxygen demand (BOD) and total suspended solids (TSS). This research provides valuable insights into sustainable biogas production practices and offers opportunities to improve energy efficiency and environmental sustainability in the palm oil industry.
{"title":"Predictive Modelling of H<sub>2</sub>S Removal from Biogas Generated from Palm Oil Mill Effluent (POME) Using a Biological Scrubber in an Industrial Biogas Plant: Integration of Artificial Neural Network (ANN) and Process Simulation<sup>§</sup>.","authors":"Joanna Lisa Clifford, Yi Jing Chan, Mohd Amran Bin Mohd Yusof, Timm Joyce Tiong, Siew Shee Lim, Chai Siah Lee, Woei-Yenn Tong","doi":"10.17113/ftb.63.02.25.8792","DOIUrl":"10.17113/ftb.63.02.25.8792","url":null,"abstract":"<p><strong>Research background: </strong>Biogas production from palm oil mill effluent (POME) is inherently unstable due to variations in feedstock composition and operating conditions. These fluctuations can lead to inconsistent biogas quality, variable methane content and fluctuating hydrogen sulphide (H<sub>2</sub>S) concentration. This poses significant challenges for bioscrubbers in removing H<sub>2</sub>S to meet quality standards for gas engines used for electricity generation. This research aims to address these challenges by integrating simulation models with a computer programme and artificial neural network (ANN) to predict the performance of a bioscrubber at a POME treatment plant in Johor, Malaysia.</p><p><strong>Experimental approach: </strong>First, the process flowsheet model was simulated using a computer programme. The H<sub>2</sub>S removal was then predicted using a machine learning algorithm, specifically ANN, based on two years of historical data obtained from the biogas plant. A detailed techno-economic analysis was also carried out to determine the economic feasibility of the process.</p><p><strong>Results and conclusions: </strong>The simulation results showed a biogas yield of 26.12 Nm<sup>3</sup> per m<sup>3</sup> POME, which is in line with industry data with less than 1 % deviation. The ANN model achieved a high coefficient of determination (R<sup>2</sup>) of 0.9 and a low mean squared error (MSE), with the bioscrubber reaching an H<sub>2</sub>S removal efficiency of approx. 96 %. The techno-economic analysis showed that the process is feasible with a net present value (NPV) of $131 000 and a payback period of 7 years.</p><p><strong>Novelty and scientific contribution: </strong>The integration of ANN and the computer programme provides a robust framework for predicting bioscrubber performance and ensuring stable bioscrubber operation due to their complementary strengths. ANN accurately predicts H<sub>2</sub>S removal based on daily recorded data, while the computer programme estimates parameters that are not monitored daily, such as chemical oxygen demand (COD), biological oxygen demand (BOD) and total suspended solids (TSS). This research provides valuable insights into sustainable biogas production practices and offers opportunities to improve energy efficiency and environmental sustainability in the palm oil industry.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"124-133"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><strong>Research background: </strong>Black garlic has been reported to have several health-promoting properties compared to fresh, raw garlic. The enzyme alliinase, which converts alliin to allicin, is deactivated at moderately high temperature, thus stripping away the typical pungent odour of fresh garlic during fermentation and rendering black garlic devoid of the typical garlic-like smell. To date, the antimicrobial activity of alliin-rich extract obtained from black garlic powder has not been reported. The objectives of this study are to explore the antibacterial/antifungal activity of alliin-rich black garlic extract against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Candida albicans</i>, and to formulate a topical drug, based on the efficacy of the extract, using non-toxic, green ingredients in the form of a nanogel with promising wound-healing property and safe for human use.</p><p><strong>Experimental approach: </strong>Authenticated fresh garlic (<i>Allium sativum</i>) cloves were first fermented to yield black garlic. After fermentation, the brownish-black garlic cloves were peeled and ground into powder. The alliin-rich extract was then obtained by Soxhlet extraction. Nanogels were formulated using the alliin-rich extract and were subjected to a kinetic study of <i>in vitro</i> release. The antibacterial potency of the nanogels was also evaluated against <i>Staphylococcus aureus</i> (ATCC 29213) and <i>Escherichia coli</i> (ATCC 25922 and their multiple drug-resistant strains), followed by a skin irritation study on New Zealand albino rabbits.</p><p><strong>Results and conclusions: </strong>Soxhlet extraction of pulverized black garlic cloves using distilled water yielded an alliin-rich extract (6.4 mg/100 g garlic), which also contained additional bioactive organosulfur compounds with no reported toxicity. The antimicrobial potency (in terms of its minimum inhibitory concentration (MIC)) of the extract was evaluated against potent skin pathogens and was found to be ~15 µg/mL. The nanogels formulated with the alliin-rich extract showed shear thinning rheology and admirable sensory properties when tested by a panel. The <i>in vitro</i> release kinetic study showed a burst release of alliin (75 % of its content) from either gel within 5 min. Following a skin irritation test performed on male New Zealand albino rabbits, no clinical signs of toxicity/mortality, redness or swelling were observed in the animals. The nanogels applied individually on the epidermal wounds prevented external infection and accelerated wound healing from day 2 onwards, achieving complete healing by day 7. Moreover, the gel containing 4 % extract did not leave a scar on the wounded area after complete healing on day 7, establishing it as a promising topical antibacterial nanogel with accelerated epidermal wound-healing property, compared to a commercial broad-spectrum topical gel, used as a positive control.</p><p><strong>Novelty and scientifi
{"title":"Development and Application of a Novel 'Green' Antibacterial Black Garlic (<i>Allium sativum</i>)-Based Nanogel in Epidermal Wound Healing<sup>§</sup>.","authors":"Mariah Sadaf, Anamika Das, Satadal Das, Subhankar Saha, Ketousetuo Kuotsu, Paramita Bhattacharjee","doi":"10.17113/ftb.63.02.25.8873","DOIUrl":"10.17113/ftb.63.02.25.8873","url":null,"abstract":"<p><strong>Research background: </strong>Black garlic has been reported to have several health-promoting properties compared to fresh, raw garlic. The enzyme alliinase, which converts alliin to allicin, is deactivated at moderately high temperature, thus stripping away the typical pungent odour of fresh garlic during fermentation and rendering black garlic devoid of the typical garlic-like smell. To date, the antimicrobial activity of alliin-rich extract obtained from black garlic powder has not been reported. The objectives of this study are to explore the antibacterial/antifungal activity of alliin-rich black garlic extract against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Candida albicans</i>, and to formulate a topical drug, based on the efficacy of the extract, using non-toxic, green ingredients in the form of a nanogel with promising wound-healing property and safe for human use.</p><p><strong>Experimental approach: </strong>Authenticated fresh garlic (<i>Allium sativum</i>) cloves were first fermented to yield black garlic. After fermentation, the brownish-black garlic cloves were peeled and ground into powder. The alliin-rich extract was then obtained by Soxhlet extraction. Nanogels were formulated using the alliin-rich extract and were subjected to a kinetic study of <i>in vitro</i> release. The antibacterial potency of the nanogels was also evaluated against <i>Staphylococcus aureus</i> (ATCC 29213) and <i>Escherichia coli</i> (ATCC 25922 and their multiple drug-resistant strains), followed by a skin irritation study on New Zealand albino rabbits.</p><p><strong>Results and conclusions: </strong>Soxhlet extraction of pulverized black garlic cloves using distilled water yielded an alliin-rich extract (6.4 mg/100 g garlic), which also contained additional bioactive organosulfur compounds with no reported toxicity. The antimicrobial potency (in terms of its minimum inhibitory concentration (MIC)) of the extract was evaluated against potent skin pathogens and was found to be ~15 µg/mL. The nanogels formulated with the alliin-rich extract showed shear thinning rheology and admirable sensory properties when tested by a panel. The <i>in vitro</i> release kinetic study showed a burst release of alliin (75 % of its content) from either gel within 5 min. Following a skin irritation test performed on male New Zealand albino rabbits, no clinical signs of toxicity/mortality, redness or swelling were observed in the animals. The nanogels applied individually on the epidermal wounds prevented external infection and accelerated wound healing from day 2 onwards, achieving complete healing by day 7. Moreover, the gel containing 4 % extract did not leave a scar on the wounded area after complete healing on day 7, establishing it as a promising topical antibacterial nanogel with accelerated epidermal wound-healing property, compared to a commercial broad-spectrum topical gel, used as a positive control.</p><p><strong>Novelty and scientifi","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"177-189"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An increasing amount of plastics is being used due to the growing population. Plastic waste pollution has become a major problem, especially in the marine environment, due to the increasing global demand for plastic materials. Bioplastics produced from waste in biorefineries offer a sustainable alternative to traditional plastics by recycling materials that are normally thrown away in the food, farming and manufacturing industries. This technology tackles both the plastic waste crisis and the inefficient use of biomass. By recycling biorefinery waste into bioplastics, the impact on the environment can be reduced, waste minimised and less fossil fuel consumed. Improving material qualities, reducing production costs and optimising the efficiency and scalability of these processes are all ongoing challenges. This review focuses on waste biorefineries for bioplastic synthesis as a sustainable approach to the circular bioeconomy. It also provides a better understanding of environmental sustainability, societal well-being and technological advances in the utilisation of various biorefineries as different substrates and methods for bioplastic synthesis.
{"title":"An Overview of Biorefinery Waste for Microbial Production of Green Plastic in a Circular Economy<sup>§</sup>.","authors":"Geethika Gudapati, Sridevi Veluru, Tanmayi Bora, M Tukaram Bai, Anupama Kavya Priya Dwarapureddy, Giri Prasad Reddi, Husam Talib Hamzah","doi":"10.17113/ftb.63.02.25.8966","DOIUrl":"10.17113/ftb.63.02.25.8966","url":null,"abstract":"<p><p>An increasing amount of plastics is being used due to the growing population. Plastic waste pollution has become a major problem, especially in the marine environment, due to the increasing global demand for plastic materials. Bioplastics produced from waste in biorefineries offer a sustainable alternative to traditional plastics by recycling materials that are normally thrown away in the food, farming and manufacturing industries. This technology tackles both the plastic waste crisis and the inefficient use of biomass. By recycling biorefinery waste into bioplastics, the impact on the environment can be reduced, waste minimised and less fossil fuel consumed. Improving material qualities, reducing production costs and optimising the efficiency and scalability of these processes are all ongoing challenges. This review focuses on waste biorefineries for bioplastic synthesis as a sustainable approach to the circular bioeconomy. It also provides a better understanding of environmental sustainability, societal well-being and technological advances in the utilisation of various biorefineries as different substrates and methods for bioplastic synthesis.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"220-237"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Turning Agricultural Waste into Useful Biochemicals and Biofuels Through Biochemical Engineering and Biotechnological Processing.","authors":"Jun Wei Lim","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"122-123"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.17113/ftb.63.02.25.9020
Abu Danish Aiman Bin Abu Sofian, Vincent Lee, Henry Marn Jhun Leong, Yeong Shenq Lee, Guan-Ting Pan, Yi Jing Chan
Research background: The increasing environmental concerns due to fossil fuel consumption and industrial wastewater pollution necessitate sustainable solutions for bioenergy production and wastewater treatment. Palm oil mill effluent (POME), a high-strength industrial wastewater, poses significant environmental challenges. Microbial electrolysis cells (MEC) and microbial fuel cells (MFC) offer promising avenues for bioenergy recovery from such wastewaters.
Experimental approach: Dual-chamber H-type reactors equipped with proton exchange membranes were used to separately evaluate the performance of MEC and MFC in the production of bioenergy from POME. Hydrogen production and chemical oxygen demand (COD) removal in MECs were evaluated at different applied voltages and influent COD expressed as oxygen concentrations, while in MFCs the effect of external resistance on power output and COD reduction was investigated. Response surface methodology (RSM) was used to optimise these operational parameters for maximum bioenergy recovery and efficient wastewater treatment.
Results and conclusions: The results showed that the efficiency of hydrogen production and COD removal in MECs were maximised at low influent COD value and low voltage supply. The MEC effectively produced hydrogen and treated industrial wastewater, while the MFC successfully produced electricity and reduced COD. Field emission scanning electron microscopy confirmed the formation of biofilms on the electrodes, indicating active microbial communities involved in the production of bioenergy. A trade-off between power density and COD removal efficiency in MFCs was observed, with medium resistance values yielding maximum power output. The integration of MEC and MFC showed potential for treating high-strength industrial wastewater like POME, offering a greener and more energy-efficient approach.
Novelty and scientific contribution: This study demonstrates the potential feasibility of integrating MEC and MFC technologies for simultaneous bioenergy production and wastewater treatment from POME. It extends the knowledge in biochemical engineering by optimising operational conditions for improved bioenergy recovery and highlights the role of microbial communities in bioelectrochemical systems. The results form a basis for future research on sustainable bioenergy production and contribute to efforts towards environmental sustainability.
{"title":"A Comparative Study of Microbial Fuel Cells and Microbial Electrolysis Cells for Bioenergy Production from Palm Oil Mill Effluent<sup>§</sup>.","authors":"Abu Danish Aiman Bin Abu Sofian, Vincent Lee, Henry Marn Jhun Leong, Yeong Shenq Lee, Guan-Ting Pan, Yi Jing Chan","doi":"10.17113/ftb.63.02.25.9020","DOIUrl":"10.17113/ftb.63.02.25.9020","url":null,"abstract":"<p><strong>Research background: </strong>The increasing environmental concerns due to fossil fuel consumption and industrial wastewater pollution necessitate sustainable solutions for bioenergy production and wastewater treatment. Palm oil mill effluent (POME), a high-strength industrial wastewater, poses significant environmental challenges. Microbial electrolysis cells (MEC) and microbial fuel cells (MFC) offer promising avenues for bioenergy recovery from such wastewaters.</p><p><strong>Experimental approach: </strong>Dual-chamber H-type reactors equipped with proton exchange membranes were used to separately evaluate the performance of MEC and MFC in the production of bioenergy from POME. Hydrogen production and chemical oxygen demand (COD) removal in MECs were evaluated at different applied voltages and influent COD expressed as oxygen concentrations, while in MFCs the effect of external resistance on power output and COD reduction was investigated. Response surface methodology (RSM) was used to optimise these operational parameters for maximum bioenergy recovery and efficient wastewater treatment.</p><p><strong>Results and conclusions: </strong>The results showed that the efficiency of hydrogen production and COD removal in MECs were maximised at low influent COD value and low voltage supply. The MEC effectively produced hydrogen and treated industrial wastewater, while the MFC successfully produced electricity and reduced COD. Field emission scanning electron microscopy confirmed the formation of biofilms on the electrodes, indicating active microbial communities involved in the production of bioenergy. A trade-off between power density and COD removal efficiency in MFCs was observed, with medium resistance values yielding maximum power output. The integration of MEC and MFC showed potential for treating high-strength industrial wastewater like POME, offering a greener and more energy-efficient approach.</p><p><strong>Novelty and scientific contribution: </strong>This study demonstrates the potential feasibility of integrating MEC and MFC technologies for simultaneous bioenergy production and wastewater treatment from POME. It extends the knowledge in biochemical engineering by optimising operational conditions for improved bioenergy recovery and highlights the role of microbial communities in bioelectrochemical systems. The results form a basis for future research on sustainable bioenergy production and contribute to efforts towards environmental sustainability.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"206-219"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The cultivation of citrus fruits has increased significantly around the globe due to rising consumer demand. The citrus fruit processing industry produces approx. 110 to 120 million tonnes of citrus fruit waste worldwide every year. This in turn contributes to landfills and environmental pollution, and poses a risk to human health and the ecosystem. Proper recycling of citrus waste helps reduce pollution and also serves as a sustainable source for the production of different bio-based products. Abundant bioactive compounds in citrus waste offer immense economic value for the production of various useful products. Moreover, bioactive compounds found in citrus wastes have various biological properties, including antioxidant, anticancer, antimutagenic, antiplatelet, cardioprotective and antiviral activities. Instead of disposing of them directly, citrus wastes can be upcycled into various value-added products, including single-cell proteins, biopolymers, pectin, biofuel, biofertilizer and bioenergy. Citrus peels serve as a cost-effective reservoir of nutraceuticals and provide an affordable dietary option for the treatment of degenerative diseases. The citrus waste, which is used as a biofertilizer and is a rich source of phenolic compounds and carotenoids, helps to extend the shelf life of food. The aim is to maintain economic viability and sustainability with the help of recent innovations in the industry. This review discusses recent advances in the valorization of citrus fruit waste and presents innovative biotechnological approaches to extract valuable bioactive compounds such as limonene, flavonoids and pectin. These compounds are used in different sectors, from the food and pharmaceutical industries to bioenergy. Techniques such as microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) are characterized by high yields and energy efficiency. Techniques for sampling, pretreatment, extraction of phytochemicals, purification and identification of citrus fruit waste are also studied. Additionally, this review highlights the environmental benefits of waste valorization as part of a circular economy approach that contributes to both economic sustainability and pollution reduction.
{"title":"A Review on Innovative Biotechnological Approaches for the Upcycling of Citrus Fruit Waste to Obtain Value-Added Bioproducts<sup>§</sup>.","authors":"Mahalingam Divyasakthi, Yerasala Charu Lekha Sarayu, Dilip Kumar Shanmugam, Guruviah Karthigadevi, Ramasamy Subbaiya, Natchimuthu Karmegam, J Jessica Kaaviya, Woo Jin Chung, Soon Woong Chang, Balasubramani Ravindran, Kuan Shiong Khoo","doi":"10.17113/ftb.63.02.25.8735","DOIUrl":"10.17113/ftb.63.02.25.8735","url":null,"abstract":"<p><p>The cultivation of citrus fruits has increased significantly around the globe due to rising consumer demand. The citrus fruit processing industry produces approx. 110 to 120 million tonnes of citrus fruit waste worldwide every year. This in turn contributes to landfills and environmental pollution, and poses a risk to human health and the ecosystem. Proper recycling of citrus waste helps reduce pollution and also serves as a sustainable source for the production of different bio-based products. Abundant bioactive compounds in citrus waste offer immense economic value for the production of various useful products. Moreover, bioactive compounds found in citrus wastes have various biological properties, including antioxidant, anticancer, antimutagenic, antiplatelet, cardioprotective and antiviral activities. Instead of disposing of them directly, citrus wastes can be upcycled into various value-added products, including single-cell proteins, biopolymers, pectin, biofuel, biofertilizer and bioenergy. Citrus peels serve as a cost-effective reservoir of nutraceuticals and provide an affordable dietary option for the treatment of degenerative diseases. The citrus waste, which is used as a biofertilizer and is a rich source of phenolic compounds and carotenoids, helps to extend the shelf life of food. The aim is to maintain economic viability and sustainability with the help of recent innovations in the industry. This review discusses recent advances in the valorization of citrus fruit waste and presents innovative biotechnological approaches to extract valuable bioactive compounds such as limonene, flavonoids and pectin. These compounds are used in different sectors, from the food and pharmaceutical industries to bioenergy. Techniques such as microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) are characterized by high yields and energy efficiency. Techniques for sampling, pretreatment, extraction of phytochemicals, purification and identification of citrus fruit waste are also studied. Additionally, this review highlights the environmental benefits of waste valorization as part of a circular economy approach that contributes to both economic sustainability and pollution reduction.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"238-261"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}