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Comparative Study on Composting and Vermicomposting to Improve Physicochemical Properties of Digestate with the Addition of Kitchen Waste§. 厨余垃圾加入堆肥与蚯蚓堆肥改善食糜理化性能的比较研究[j]。
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8904
Ze Sen Tan, M Devendran Manogaran, Rashid Shamsuddin, Mohd Hakimi, Lee Wen Looi, Kai Tong Woo, Chin Seng Liew, Lailatul Qomariyah

Research background: The rapid growth of the Malaysian population has led to an increase in kitchen waste, especially inedible organic kitchen waste, which is generally disposed of in landfills and pollutes the environment. Apart from this, the increasing demand for chicken products in Malaysia has led to a significant increase in chicken manure production. As anaerobic digestion continues to be explored, there are concerns about the utilization of the digestate from chicken manure. Therefore, this study addresses the challenge of treating kitchen waste and chicken manure digestate in Malaysia by investigating the effectiveness of composting and vermicomposting methods through comparative analysis. By integrating kitchen waste, particularly spent coffee grounds, bone waste and used kitchen towels, this study aims to improve the imbalanced physicochemical properties of digestate from chicken manure.

Experimental approach: Before composting, the kitchen waste and chicken manure digestate were characterised to determine the initial physicochemical properties. Four composting setups comprising the substances were established to study the physical appearance, temperature and pH profile, the increase in nitrogen, phosphorus and potassium content, and the mass reduction of the final compost after 50 days of composting.

Results and conclusions: The vermicompost with kitchen waste additives showed a significant nutrient improvement with an NPK mass ratio of 1:3.57:6.58 and a lower moisture mass fraction of 48.92 %, which requires the shortest maturation time (20 days) and the highest mass reduction (55.11 %).

Novelty and scientific contribution: The novelty of this research is the valorisation of organic kitchen waste and chicken manure digestate as biofertiliser. The end result is achieved by promoting a sustainable alternative to exploit kitchen waste instead of the traditional approach of landfilling waste. At the same time the problem of digestate is addressed, particularly its unbalanced physicochemical properties, especially its macronutrients, pH and moisture content. In contrast to previous studies, this work investigates the effectiveness of both conventional composting and vermicomposting with the incorporation of organic kitchen waste, namely spent coffee grounds, bone meal and used kitchen towels, to improve the physicochemical properties of digestate.

研究背景:马来西亚人口的快速增长导致厨余垃圾的增加,特别是不可食用的有机厨余垃圾,这些垃圾通常被填埋,污染环境。除此之外,马来西亚对鸡肉产品的需求不断增加,导致鸡粪产量大幅增加。随着厌氧消化的不断探索,人们开始关注鸡粪消化液的利用。因此,本研究通过比较分析调查堆肥和蠕虫堆肥方法的有效性,解决了马来西亚处理厨房垃圾和鸡粪消化的挑战。本研究旨在通过整合厨余垃圾,特别是废咖啡渣、废骨头和用过的厨房毛巾,改善鸡粪消化物不平衡的理化性质。实验方法:在堆肥前,对餐厨垃圾和鸡粪消化液进行表征,测定其初始理化性质。建立了四种堆肥装置,研究了堆肥50天后的物理外观、温度和pH曲线、氮、磷和钾含量的增加以及最终堆肥的质量减少。结果与结论:添加餐厨垃圾后的蚯蚓堆肥的氮磷钾质量比为1:3.57:6.58,水分质量分数为48.92%,其成熟时间最短(20 d),质量降幅最大(55.11%)。新颖性和科学贡献:这项研究的新颖性是有机厨房垃圾和鸡粪消化作为生物肥料的价值。最终的结果是通过推广一种可持续的替代方案来利用厨余,而不是传统的填埋废物的方法。同时解决了消化的问题,特别是其不平衡的物理化学性质,特别是其常量营养素,pH值和水分含量。与之前的研究相反,本研究调查了传统堆肥和蚯蚓堆肥的有效性,并结合有机厨房垃圾,即废咖啡渣,骨粉和用过的厨房毛巾,来改善消化物的理化性质。
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引用次数: 0
Optimisation of Ultrasound-Assisted Extraction Conditions Using Response Surface Methodology and Identification of Thymoquinone from Black Cumin (Nigella sativa L.) Seed Extract§. 响应面法优化黑孜然中百里醌的超声辅助提取条件及鉴定种子提取§。
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8560
Nita Kaushik, Aradhita Barmanray
<p><strong>Research background: </strong><i>Nigella sativa</i> L., commonly known as black cumin, is a medicinal plant renowned for its rich bioactive composition and health-promoting properties. Among its key compounds, thymoquinone has gained significant attention in nutraceutical and pharmaceutical research for its potential to prevent and manage chronic inflammatory conditions and immune dysfunctions. With growing global interest in natural health solutions, the aim of this study is to optimise ultrasound-assisted extraction (UAE) conditions to maximise thymoquinone yield from the extract of black cumin (<i>Nigella sativa</i> L.) seeds and characterise the bioactive compounds. By using UAE and advanced analytical techniques, the research contributes to the development of sustainable extracts rich in bioactive compounds with applications in medicine and nutrition.</p><p><strong>Experimental approach: </strong>In this study, ultrasound-assisted extraction method was used with response surface methodology (RSM) software to extract the bioactive compounds, including total phenolic content (TPC) and compounds that can bind free DPPH radical. To increase the extraction efficiency of bioactive compounds, the following parameters were examined: the ratio of the mass of seed powder to the volume of solvent of 50-100 %, extraction temperature of 30 °C, amplitude of 30-60 % and extraction time of 30-60 min. Black cumin seed extracts were characterised using scanning electron microscopy (SEM), while gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify thymoquinone. Additionally, Fourier transform infrared (FTIR) spectroscopy confirmed the presence of thymoquinone and several functional groups, including amines, alkanes, acids, esters, alkyls and alkenes.</p><p><strong>Results and conclusions: </strong>Ultrasonic extraction using methanol as a solvent resulted in a higher yield of thymoquinone (28.62 %), identified using GC-MS analysis. The presence of thymoquinone was further confirmed by the functional groups detected in FTIR analysis. Under the specified extraction conditions, total phenolic content (TPC, expressed as gallic acid equivalents), yield (in %) and DPPH radical scavenging activity increased by approx. 271.03 mg/g, and 4.5 and 83.06 %, respectively. In addition to thymoquinone, thymohydroquinone was also identified based on its molecular mass, retention time and peak values. Thymoquinone, a natural and potent phytochemical, offers a range of therapeutic properties, including immune-enhancing potential.</p><p><strong>Novelty and scientific contribution: </strong>Thymoquinone is a bioactive compound found in black cumin seeds, known for its potent antioxidant and immunity boosting properties. This research was conducted achieve the best possible extraction conditions for bioactive substances. Additionally, the results support the potential of thymoquinone as a therapeutic agent to treat various health conditions. T
研究背景:Nigella sativa L.,俗称黑孜然,是一种药用植物,以其丰富的生物活性成分和促进健康的特性而闻名。在其主要化合物中,百里醌因其预防和治疗慢性炎症和免疫功能障碍的潜力而在营养保健和制药研究中受到了极大的关注。随着全球对天然健康解决方案的兴趣日益浓厚,本研究的目的是优化超声辅助提取(UAE)条件,以最大限度地从黑孜然(Nigella sativa L.)种子提取物中提取百里香醌,并表征其生物活性化合物。通过使用阿联酋和先进的分析技术,该研究有助于开发富含生物活性化合物的可持续提取物,并在医学和营养方面应用。实验方法:本研究采用超声辅助提取法,结合响应面法(RSM)软件提取总酚含量(TPC)和结合游离DPPH自由基的活性化合物。为了提高生物活性物质的提取效率,考察了种子粉质量与溶剂体积的比为50- 100%,提取温度为30℃,振幅为30- 60%,提取时间为30-60 min。采用扫描电镜(SEM)对黑孜然种子提取物进行了表征,采用气相色谱-质谱(GC-MS)分析对百里醌进行了鉴定。此外,傅里叶变换红外光谱(FTIR)证实了百里香醌和几个官能团的存在,包括胺、烷烃、酸、酯、烷基和烯烃。结果与结论:以甲醇为溶剂,超声提取百里醌得率较高(28.62%),经气相色谱-质谱分析鉴定。在FTIR中检测到的官能团进一步证实了百里醌的存在。在指定的提取条件下,总酚含量(TPC,以没食子酸当量表示)、产率(以%表示)和清除DPPH自由基的活性提高了约50%。271.03 mg/g、4.5%和83.06%。除百里醌外,还根据其分子量、保留时间和峰值对其进行了鉴定。百里醌是一种天然而有效的植物化学物质,具有一系列治疗特性,包括增强免疫的潜力。创新和科学贡献:百里醌是一种在黑孜然种子中发现的生物活性化合物,以其强大的抗氧化和增强免疫力而闻名。本研究旨在获得最佳提取条件。此外,研究结果支持百里醌作为治疗各种健康状况的药物的潜力。创新在于开发和优化提取技术,以最大限度地提高百里醌的产量和生物活性,百里醌是一种以其强大的抗氧化和免疫调节特性而闻名的化合物。这项工作独特地弥合了黑孜然的传统使用与现代科学验证之间的差距,并解决了全球卫生重点问题。研究结果强调了黑草作为促进健康化合物的可持续和天然来源的重要性,满足了预防保健中对植物性生物活性化合物日益增长的需求。通过对百里醌提取条件的刻画和对其治疗潜力的论证,本研究为功能食品和营养保健品的开发提供了科学文献和实践上的帮助。
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引用次数: 0
Turning Waste into Taste: Effective Upcycling of By-Products for Innovative Food Solutions§. 将废物转化为味道:创新食品解决方案副产品的有效升级利用
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8962
Swapna Sree Meduri, Sujatha Mudawath, Prabhakar Butti, Soujanya Kanneboina, Sucharita Devi Tattepalli, Supraja Thoomati, Neela Rani Rathod, Aparna Kuna, Krishna Lavuri, Srinivasa Chary Darshanoju, Kanmani Kalaivanan

Waste management in the food manufacturing sector has become one of the most challenging aspects globally owing to the generation of enormous quantities of by-products, such as peels, seeds and undesirable flesh at various stages of the processing chain. However, these plant by-products are rich in important compounds particularly polyphenols and bioactive substances that significantly affect human health and can be utilised in numerous sectors as new, low-cost and economical raw ingredients. The aim of this review paper is to discuss various methods of valorising food waste, concentrating on upcycling, aquafaba, coffee silver skin, propolis, wine lees and avocado waste. Food waste is a substantial global issue, with the potential to affect food security, environment and economy. Upcycling is highlighted as a means to tackle food waste by repurposing high-value by-products such as fruit and vegetable residues. Aquafaba, a vegan alternative to egg white, is produced from chickpeas and has various culinary applications. Coffee silver skin, a by-product of coffee production, contains bioactive compounds that can be extracted and used in functional foods. Propolis, a resinous substance collected by bees, is rich in bioactive compounds with health benefits. Wine lees, a by-product of winemaking, can be processed to extract phenolic compounds and produce value-added products. Avocado waste valorisation focuses on converting avocado by-products into valuable products for various industries. The sustainable valorisation of food waste offers numerous benefits, such as reducing waste output, generating revenue and promoting resource efficiency. Collaboration between stakeholders is essential to advance research and implement sustainable management practices for food waste valorisation to achieve the Sustainable Development Goals (SDGs). Challenges such as scaling-up, regulatory frameworks, logistics, food safety and environmental impact must be addressed to effectively valorise food waste.

食品制造部门的废物管理已成为全球最具挑战性的方面之一,因为在加工链的各个阶段会产生大量的副产品,如果皮、种子和不受欢迎的果肉。然而,这些植物副产品富含重要化合物,特别是多酚和生物活性物质,对人类健康有重大影响,可以作为新的、低成本和经济的原料在许多部门加以利用。这篇综述的目的是讨论食物垃圾的各种增值方法,集中在升级回收,水法,咖啡银皮,蜂胶,酒渣和鳄梨废物。食物浪费是一个严重的全球性问题,有可能影响粮食安全、环境和经济。升级回收是通过重新利用水果和蔬菜残渣等高价值副产品来解决食物浪费的一种手段。Aquafaba是一种纯素蛋清替代品,由鹰嘴豆制成,有多种烹饪用途。咖啡银皮是咖啡生产的副产品,含有生物活性化合物,可以提取并用于功能性食品。蜂胶是一种由蜜蜂采集的树脂状物质,富含有益健康的生物活性化合物。酒糟是酿酒的副产品,可以加工提取酚类化合物,生产增值产品。牛油果废物增值侧重于将牛油果副产品转化为各种行业的有价值产品。食物垃圾的可持续增值提供了许多好处,例如减少废物产出,创造收入和提高资源效率。为了实现可持续发展目标,利益攸关方之间的合作对于推进食物垃圾价值评估的研究和实施可持续管理实践至关重要。必须解决诸如扩大规模、监管框架、物流、食品安全和环境影响等挑战,以有效地遏制食物浪费。
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引用次数: 0
Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum §. 利用代谢工程谷氨酸棒状杆菌从单糖、木质和草本植物水解物中生产琥珀酸
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8808
Dae-Seok Lee, Eun Jin Cho, Seryung Kim, Dien Thanh Nguyen, Hyeun-Jong Bae

Research background: Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:SucE12-ΔldhA, a strain overexpressing the succinic acid transporter (SucE).

Experimental approach: The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass - bamboo, oak, poplar, pine and spent coffee grounds - were analysed to determine the most favourable bioresource for succinic acid production.

Results and conclusions: Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38-24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness.

Novelty and scientific contribution: This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability.

研究背景:木质纤维素生物质琥珀酸是一种可持续的生化生产替代品,是一种环境友好型的石油基化学品替代品。本研究的目的是评估木本植物和草本植物微纤维中半纤维素含量和纤维素纤维结构的变化对Psod:SucE12-ΔldhA酶解糖化和琥珀酸生产效率的影响,Psod:SucE12-ΔldhA是一种过表达琥珀酸转运体(SucE)的菌株。实验方法:研究了不同单糖组合对琥珀酸生成的影响,重点研究了与甘露糖组合与单独葡萄糖的对比。此外,还分析了不同木质纤维素生物质(竹、橡树、杨树、松树和废咖啡渣)的水解物,以确定生产琥珀酸的最有利生物资源。结果与结论:含甘露糖的单糖组合产生的琥珀酸比单独葡萄糖高2.20-2.48倍,表明甘露糖对琥珀酸代谢有积极影响。在木质纤维素生物质水解物中,竹子的木糖含量高于木本植物,是琥珀酸生产效率最高的生物资源(24 h内为23.38 ~ 24.12 g/L),其次是橡树、杨树、松树和废咖啡渣。因此,提高木糖消耗率对于提高木质纤维素生物质琥珀酸产量和提高市场竞争力至关重要。新颖性和科学贡献:这项研究强调了木质纤维素生物质,特别是竹子,作为琥珀酸生产的可持续原料的潜力。该研究的新颖之处在于详细研究了半纤维素含量和纤维素纤维结构如何影响酶催化糖化和发酵。甘露糖和木糖对琥珀酸产率的显著影响为生物化学生产中生物质利用的优化提供了关键见解。这些发现促进了生物化学生产,减少了对化石燃料的依赖,提高了工业的可持续性。
{"title":"Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered <i>Corynebacterium glutamicum</i> <sup>§</sup>.","authors":"Dae-Seok Lee, Eun Jin Cho, Seryung Kim, Dien Thanh Nguyen, Hyeun-Jong Bae","doi":"10.17113/ftb.63.02.25.8808","DOIUrl":"10.17113/ftb.63.02.25.8808","url":null,"abstract":"<p><strong>Research background: </strong>Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:<i>SucE</i>12-<i>ΔldhA</i>, a strain overexpressing the succinic acid transporter (<i>SucE</i>).</p><p><strong>Experimental approach: </strong>The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass - bamboo, oak, poplar, pine and spent coffee grounds - were analysed to determine the most favourable bioresource for succinic acid production.</p><p><strong>Results and conclusions: </strong>Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38-24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness.</p><p><strong>Novelty and scientific contribution: </strong>This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"134-148"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predictive Modelling of H2S Removal from Biogas Generated from Palm Oil Mill Effluent (POME) Using a Biological Scrubber in an Industrial Biogas Plant: Integration of Artificial Neural Network (ANN) and Process Simulation§. 工业沼气厂生物洗涤器去除棕榈油厂废水(POME)中H2S的预测建模:人工神经网络(ANN)和过程模拟的集成。
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8792
Joanna Lisa Clifford, Yi Jing Chan, Mohd Amran Bin Mohd Yusof, Timm Joyce Tiong, Siew Shee Lim, Chai Siah Lee, Woei-Yenn Tong

Research background: Biogas production from palm oil mill effluent (POME) is inherently unstable due to variations in feedstock composition and operating conditions. These fluctuations can lead to inconsistent biogas quality, variable methane content and fluctuating hydrogen sulphide (H2S) concentration. This poses significant challenges for bioscrubbers in removing H2S to meet quality standards for gas engines used for electricity generation. This research aims to address these challenges by integrating simulation models with a computer programme and artificial neural network (ANN) to predict the performance of a bioscrubber at a POME treatment plant in Johor, Malaysia.

Experimental approach: First, the process flowsheet model was simulated using a computer programme. The H2S removal was then predicted using a machine learning algorithm, specifically ANN, based on two years of historical data obtained from the biogas plant. A detailed techno-economic analysis was also carried out to determine the economic feasibility of the process.

Results and conclusions: The simulation results showed a biogas yield of 26.12 Nm3 per m3 POME, which is in line with industry data with less than 1 % deviation. The ANN model achieved a high coefficient of determination (R2) of 0.9 and a low mean squared error (MSE), with the bioscrubber reaching an H2S removal efficiency of approx. 96 %. The techno-economic analysis showed that the process is feasible with a net present value (NPV) of $131 000 and a payback period of 7 years.

Novelty and scientific contribution: The integration of ANN and the computer programme provides a robust framework for predicting bioscrubber performance and ensuring stable bioscrubber operation due to their complementary strengths. ANN accurately predicts H2S removal based on daily recorded data, while the computer programme estimates parameters that are not monitored daily, such as chemical oxygen demand (COD), biological oxygen demand (BOD) and total suspended solids (TSS). This research provides valuable insights into sustainable biogas production practices and offers opportunities to improve energy efficiency and environmental sustainability in the palm oil industry.

研究背景:由于原料成分和操作条件的变化,棕榈油厂废水(POME)的沼气生产本身就不稳定。这些波动可能导致沼气质量不一致、甲烷含量变化和硫化氢(H2S)浓度波动。这给生物洗涤器去除H2S以满足用于发电的燃气发动机的质量标准提出了重大挑战。本研究旨在通过将模拟模型与计算机程序和人工神经网络(ANN)相结合,来预测马来西亚柔佛州一家POME处理厂的生物洗涤器的性能,从而解决这些挑战。实验方法:首先,利用计算机程序对工艺流程模型进行仿真。然后,根据从沼气厂获得的两年历史数据,使用机器学习算法(特别是人工神经网络)预测H2S的去除率。还进行了详细的技术经济分析,以确定该工艺的经济可行性。结果与结论:模拟结果显示,沼气产气量为26.12 Nm3 / m3 POME,与行业数据吻合,偏差小于1%。人工神经网络模型的决定系数(R2)为0.9,均方误差(MSE)较低,生物洗涤器的H2S去除率约为0.45。96%。技术经济分析表明,该工艺是可行的,净现值(NPV)为13.1万美元,投资回收期为7年。新颖性和科学贡献:人工神经网络和计算机程序的集成为预测生物洗涤器性能和确保生物洗涤器稳定运行提供了一个强大的框架,因为它们具有互补的优势。ANN根据每日记录数据准确预测H2S去除率,而计算机程序则估算非日常监测的参数,如化学需氧量(COD)、生物需氧量(BOD)和总悬浮固体(TSS)。这项研究为可持续沼气生产实践提供了宝贵的见解,并为提高棕榈油行业的能源效率和环境可持续性提供了机会。
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引用次数: 0
Development and Application of a Novel 'Green' Antibacterial Black Garlic (Allium sativum)-Based Nanogel in Epidermal Wound Healing§. 新型“绿色”抗菌黑蒜纳米凝胶在表皮伤口愈合中的开发与应用
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8873
Mariah Sadaf, Anamika Das, Satadal Das, Subhankar Saha, Ketousetuo Kuotsu, Paramita Bhattacharjee
<p><strong>Research background: </strong>Black garlic has been reported to have several health-promoting properties compared to fresh, raw garlic. The enzyme alliinase, which converts alliin to allicin, is deactivated at moderately high temperature, thus stripping away the typical pungent odour of fresh garlic during fermentation and rendering black garlic devoid of the typical garlic-like smell. To date, the antimicrobial activity of alliin-rich extract obtained from black garlic powder has not been reported. The objectives of this study are to explore the antibacterial/antifungal activity of alliin-rich black garlic extract against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Candida albicans</i>, and to formulate a topical drug, based on the efficacy of the extract, using non-toxic, green ingredients in the form of a nanogel with promising wound-healing property and safe for human use.</p><p><strong>Experimental approach: </strong>Authenticated fresh garlic (<i>Allium sativum</i>) cloves were first fermented to yield black garlic. After fermentation, the brownish-black garlic cloves were peeled and ground into powder. The alliin-rich extract was then obtained by Soxhlet extraction. Nanogels were formulated using the alliin-rich extract and were subjected to a kinetic study of <i>in vitro</i> release. The antibacterial potency of the nanogels was also evaluated against <i>Staphylococcus aureus</i> (ATCC 29213) and <i>Escherichia coli</i> (ATCC 25922 and their multiple drug-resistant strains), followed by a skin irritation study on New Zealand albino rabbits.</p><p><strong>Results and conclusions: </strong>Soxhlet extraction of pulverized black garlic cloves using distilled water yielded an alliin-rich extract (6.4 mg/100 g garlic), which also contained additional bioactive organosulfur compounds with no reported toxicity. The antimicrobial potency (in terms of its minimum inhibitory concentration (MIC)) of the extract was evaluated against potent skin pathogens and was found to be ~15 µg/mL. The nanogels formulated with the alliin-rich extract showed shear thinning rheology and admirable sensory properties when tested by a panel. The <i>in vitro</i> release kinetic study showed a burst release of alliin (75 % of its content) from either gel within 5 min. Following a skin irritation test performed on male New Zealand albino rabbits, no clinical signs of toxicity/mortality, redness or swelling were observed in the animals. The nanogels applied individually on the epidermal wounds prevented external infection and accelerated wound healing from day 2 onwards, achieving complete healing by day 7. Moreover, the gel containing 4 % extract did not leave a scar on the wounded area after complete healing on day 7, establishing it as a promising topical antibacterial nanogel with accelerated epidermal wound-healing property, compared to a commercial broad-spectrum topical gel, used as a positive control.</p><p><strong>Novelty and scientifi
研究背景:据报道,与新鲜的生大蒜相比,黑蒜具有几种促进健康的特性。将蒜素转化为蒜素的蒜氨酸酶在中等高温下失活,从而在发酵过程中去除新鲜大蒜的典型刺鼻气味,使黑蒜失去了典型的大蒜味。迄今为止,从黑蒜粉中提取的富含蒜素的提取物的抗菌活性尚未见报道。本研究的目的是探索富含蒜素的黑蒜提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌/抗真菌活性,并基于该提取物的功效,采用无毒、绿色的纳米凝胶形式制备一种外用药物,具有良好的伤口愈合性能,可安全用于人体。实验方法:经鉴定的新鲜大蒜(Allium sativum)瓣首先发酵产生黑蒜。发酵后,将棕黑色的蒜瓣剥皮磨成粉末。然后用索氏提取法得到富含蒜素的提取物。利用富含蒜素的提取物配制纳米凝胶,并对其体外释放进行动力学研究。研究了纳米凝胶对金黄色葡萄球菌(ATCC 29213)和大肠埃希菌(ATCC 25922)及其多重耐药菌株的抑菌效果,并对新西兰白化兔进行了皮肤刺激试验。结果和结论:用蒸馏水对黑蒜瓣粉末进行索氏提取,得到一种富含蒜素的提取物(6.4 mg/100 g大蒜),其中还含有额外的生物活性有机硫化合物,没有毒性报道。对该提取物的抑菌效力(以其最低抑菌浓度(MIC)衡量)进行了评估,发现其对有效的皮肤病原体的抑菌效力为~15µg/mL。通过面板测试,用富含蒜素提取物配制的纳米凝胶显示出剪切变薄的流变性和令人满意的感官性能。体外释放动力学研究显示,在5分钟内,两种凝胶都能释放出大蒜素(其含量的75%)。在对雄性新西兰白化兔进行皮肤刺激试验后,动物没有观察到毒性/死亡、红肿的临床迹象。纳米凝胶单独应用于表皮伤口,从第2天开始防止外部感染并加速伤口愈合,到第7天完全愈合。此外,与作为阳性对照的商业广谱外用凝胶相比,含有4%提取物的凝胶在第7天完全愈合后不会在受伤区域留下疤痕,这表明它是一种有前途的外用抗菌纳米凝胶,具有加速表皮伤口愈合的特性。新颖性和科学贡献:本研究首次报道了新开发的含有抗菌生物活性化合物的“绿色”纳米凝胶,即有机硫化合物(二烯丙基二硫、二烯丙基三硫、甲基烯丙基二硫和甲基烯丙基三硫)。纳米凝胶显示出良好的表皮伤口愈合性能,因此在临床应用中具有抗常见和强效人体皮肤病原体的前景。
{"title":"Development and Application of a Novel 'Green' Antibacterial Black Garlic (<i>Allium sativum</i>)-Based Nanogel in Epidermal Wound Healing<sup>§</sup>.","authors":"Mariah Sadaf, Anamika Das, Satadal Das, Subhankar Saha, Ketousetuo Kuotsu, Paramita Bhattacharjee","doi":"10.17113/ftb.63.02.25.8873","DOIUrl":"10.17113/ftb.63.02.25.8873","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Research background: &lt;/strong&gt;Black garlic has been reported to have several health-promoting properties compared to fresh, raw garlic. The enzyme alliinase, which converts alliin to allicin, is deactivated at moderately high temperature, thus stripping away the typical pungent odour of fresh garlic during fermentation and rendering black garlic devoid of the typical garlic-like smell. To date, the antimicrobial activity of alliin-rich extract obtained from black garlic powder has not been reported. The objectives of this study are to explore the antibacterial/antifungal activity of alliin-rich black garlic extract against &lt;i&gt;Staphylococcus aureus&lt;/i&gt;, &lt;i&gt;Escherichia coli&lt;/i&gt; and &lt;i&gt;Candida albicans&lt;/i&gt;, and to formulate a topical drug, based on the efficacy of the extract, using non-toxic, green ingredients in the form of a nanogel with promising wound-healing property and safe for human use.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Experimental approach: &lt;/strong&gt;Authenticated fresh garlic (&lt;i&gt;Allium sativum&lt;/i&gt;) cloves were first fermented to yield black garlic. After fermentation, the brownish-black garlic cloves were peeled and ground into powder. The alliin-rich extract was then obtained by Soxhlet extraction. Nanogels were formulated using the alliin-rich extract and were subjected to a kinetic study of &lt;i&gt;in vitro&lt;/i&gt; release. The antibacterial potency of the nanogels was also evaluated against &lt;i&gt;Staphylococcus aureus&lt;/i&gt; (ATCC 29213) and &lt;i&gt;Escherichia coli&lt;/i&gt; (ATCC 25922 and their multiple drug-resistant strains), followed by a skin irritation study on New Zealand albino rabbits.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results and conclusions: &lt;/strong&gt;Soxhlet extraction of pulverized black garlic cloves using distilled water yielded an alliin-rich extract (6.4 mg/100 g garlic), which also contained additional bioactive organosulfur compounds with no reported toxicity. The antimicrobial potency (in terms of its minimum inhibitory concentration (MIC)) of the extract was evaluated against potent skin pathogens and was found to be ~15 µg/mL. The nanogels formulated with the alliin-rich extract showed shear thinning rheology and admirable sensory properties when tested by a panel. The &lt;i&gt;in vitro&lt;/i&gt; release kinetic study showed a burst release of alliin (75 % of its content) from either gel within 5 min. Following a skin irritation test performed on male New Zealand albino rabbits, no clinical signs of toxicity/mortality, redness or swelling were observed in the animals. The nanogels applied individually on the epidermal wounds prevented external infection and accelerated wound healing from day 2 onwards, achieving complete healing by day 7. Moreover, the gel containing 4 % extract did not leave a scar on the wounded area after complete healing on day 7, establishing it as a promising topical antibacterial nanogel with accelerated epidermal wound-healing property, compared to a commercial broad-spectrum topical gel, used as a positive control.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Novelty and scientifi","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"63 2","pages":"177-189"},"PeriodicalIF":2.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Overview of Biorefinery Waste for Microbial Production of Green Plastic in a Circular Economy§. 循环经济下生物炼制废弃物微生物生产绿色塑料综述
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8966
Geethika Gudapati, Sridevi Veluru, Tanmayi Bora, M Tukaram Bai, Anupama Kavya Priya Dwarapureddy, Giri Prasad Reddi, Husam Talib Hamzah

An increasing amount of plastics is being used due to the growing population. Plastic waste pollution has become a major problem, especially in the marine environment, due to the increasing global demand for plastic materials. Bioplastics produced from waste in biorefineries offer a sustainable alternative to traditional plastics by recycling materials that are normally thrown away in the food, farming and manufacturing industries. This technology tackles both the plastic waste crisis and the inefficient use of biomass. By recycling biorefinery waste into bioplastics, the impact on the environment can be reduced, waste minimised and less fossil fuel consumed. Improving material qualities, reducing production costs and optimising the efficiency and scalability of these processes are all ongoing challenges. This review focuses on waste biorefineries for bioplastic synthesis as a sustainable approach to the circular bioeconomy. It also provides a better understanding of environmental sustainability, societal well-being and technological advances in the utilisation of various biorefineries as different substrates and methods for bioplastic synthesis.

由于人口的增长,塑料的使用量越来越大。由于全球对塑料材料的需求不断增加,塑料废物污染已成为一个重大问题,特别是在海洋环境中。从生物精炼厂的废物中生产的生物塑料通过回收通常在食品、农业和制造业中丢弃的材料,为传统塑料提供了一种可持续的替代品。这项技术既解决了塑料垃圾危机,也解决了生物质的低效利用。通过将生物炼制废物回收为生物塑料,可以减少对环境的影响,最大限度地减少废物,减少化石燃料的消耗。提高材料质量、降低生产成本、优化这些工艺的效率和可扩展性都是目前面临的挑战。本文重点介绍了废弃生物精炼厂用于生物塑料合成的一种可持续的循环生物经济方法。它还提供了一个更好的理解环境可持续性,社会福祉和技术进步在利用各种生物精炼厂作为不同的底物和生物塑料合成方法。
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引用次数: 0
Turning Agricultural Waste into Useful Biochemicals and Biofuels Through Biochemical Engineering and Biotechnological Processing. 通过生物化学工程和生物技术处理将农业废物转化为有用的生物化学品和生物燃料。
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01
Jun Wei Lim
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引用次数: 0
A Comparative Study of Microbial Fuel Cells and Microbial Electrolysis Cells for Bioenergy Production from Palm Oil Mill Effluent§. 微生物燃料电池和微生物电解电池用于棕榈油厂废水生物能源生产的比较研究。
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.9020
Abu Danish Aiman Bin Abu Sofian, Vincent Lee, Henry Marn Jhun Leong, Yeong Shenq Lee, Guan-Ting Pan, Yi Jing Chan

Research background: The increasing environmental concerns due to fossil fuel consumption and industrial wastewater pollution necessitate sustainable solutions for bioenergy production and wastewater treatment. Palm oil mill effluent (POME), a high-strength industrial wastewater, poses significant environmental challenges. Microbial electrolysis cells (MEC) and microbial fuel cells (MFC) offer promising avenues for bioenergy recovery from such wastewaters.

Experimental approach: Dual-chamber H-type reactors equipped with proton exchange membranes were used to separately evaluate the performance of MEC and MFC in the production of bioenergy from POME. Hydrogen production and chemical oxygen demand (COD) removal in MECs were evaluated at different applied voltages and influent COD expressed as oxygen concentrations, while in MFCs the effect of external resistance on power output and COD reduction was investigated. Response surface methodology (RSM) was used to optimise these operational parameters for maximum bioenergy recovery and efficient wastewater treatment.

Results and conclusions: The results showed that the efficiency of hydrogen production and COD removal in MECs were maximised at low influent COD value and low voltage supply. The MEC effectively produced hydrogen and treated industrial wastewater, while the MFC successfully produced electricity and reduced COD. Field emission scanning electron microscopy confirmed the formation of biofilms on the electrodes, indicating active microbial communities involved in the production of bioenergy. A trade-off between power density and COD removal efficiency in MFCs was observed, with medium resistance values yielding maximum power output. The integration of MEC and MFC showed potential for treating high-strength industrial wastewater like POME, offering a greener and more energy-efficient approach.

Novelty and scientific contribution: This study demonstrates the potential feasibility of integrating MEC and MFC technologies for simultaneous bioenergy production and wastewater treatment from POME. It extends the knowledge in biochemical engineering by optimising operational conditions for improved bioenergy recovery and highlights the role of microbial communities in bioelectrochemical systems. The results form a basis for future research on sustainable bioenergy production and contribute to efforts towards environmental sustainability.

研究背景:化石燃料消耗和工业废水污染引起的环境问题日益严重,需要可持续的生物能源生产和废水处理解决方案。棕榈油厂废水(POME)是一种高强度工业废水,对环境构成重大挑战。微生物电解电池(MEC)和微生物燃料电池(MFC)为从此类废水中回收生物能源提供了有前途的途径。实验方法:采用配备质子交换膜的双室h型反应器,分别对MEC和MFC在POME生产生物能源中的性能进行了评价。在不同的施加电压和以氧浓度表示的进水COD下,研究了mec的产氢和化学需氧量(COD)去除,而在MFCs中,研究了外部电阻对输出功率和COD降低的影响。响应面法(RSM)用于优化这些操作参数,以最大限度地回收生物能源和有效的废水处理。结果与结论:结果表明,低进水COD值和低压供电条件下,mec产氢和COD去除效率最高。MEC有效产氢并处理工业废水,MFC成功产电并降低COD。场发射扫描电镜证实了电极上生物膜的形成,表明活跃的微生物群落参与了生物能源的生产。mfc的功率密度和COD去除效率之间存在权衡,中等电阻值产生最大的功率输出。MEC和MFC的结合显示了处理POME等高强度工业废水的潜力,提供了一种更环保、更节能的方法。新颖性和科学贡献:本研究证明了将MEC和MFC技术结合起来同时生产生物能源和处理POME废水的潜在可行性。它通过优化操作条件来提高生物能源回收,扩展了生物化学工程的知识,并强调了微生物群落在生物电化学系统中的作用。这些结果为未来可持续生物能源生产的研究奠定了基础,并有助于实现环境的可持续性。
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引用次数: 0
A Review on Innovative Biotechnological Approaches for the Upcycling of Citrus Fruit Waste to Obtain Value-Added Bioproducts§. 柑橘类水果废弃物升级回收获得高附加值生物制品的创新生物技术途径综述
IF 2.5 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-06-01 DOI: 10.17113/ftb.63.02.25.8735
Mahalingam Divyasakthi, Yerasala Charu Lekha Sarayu, Dilip Kumar Shanmugam, Guruviah Karthigadevi, Ramasamy Subbaiya, Natchimuthu Karmegam, J Jessica Kaaviya, Woo Jin Chung, Soon Woong Chang, Balasubramani Ravindran, Kuan Shiong Khoo

The cultivation of citrus fruits has increased significantly around the globe due to rising consumer demand. The citrus fruit processing industry produces approx. 110 to 120 million tonnes of citrus fruit waste worldwide every year. This in turn contributes to landfills and environmental pollution, and poses a risk to human health and the ecosystem. Proper recycling of citrus waste helps reduce pollution and also serves as a sustainable source for the production of different bio-based products. Abundant bioactive compounds in citrus waste offer immense economic value for the production of various useful products. Moreover, bioactive compounds found in citrus wastes have various biological properties, including antioxidant, anticancer, antimutagenic, antiplatelet, cardioprotective and antiviral activities. Instead of disposing of them directly, citrus wastes can be upcycled into various value-added products, including single-cell proteins, biopolymers, pectin, biofuel, biofertilizer and bioenergy. Citrus peels serve as a cost-effective reservoir of nutraceuticals and provide an affordable dietary option for the treatment of degenerative diseases. The citrus waste, which is used as a biofertilizer and is a rich source of phenolic compounds and carotenoids, helps to extend the shelf life of food. The aim is to maintain economic viability and sustainability with the help of recent innovations in the industry. This review discusses recent advances in the valorization of citrus fruit waste and presents innovative biotechnological approaches to extract valuable bioactive compounds such as limonene, flavonoids and pectin. These compounds are used in different sectors, from the food and pharmaceutical industries to bioenergy. Techniques such as microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) are characterized by high yields and energy efficiency. Techniques for sampling, pretreatment, extraction of phytochemicals, purification and identification of citrus fruit waste are also studied. Additionally, this review highlights the environmental benefits of waste valorization as part of a circular economy approach that contributes to both economic sustainability and pollution reduction.

由于消费者需求的增加,柑橘类水果的种植在全球范围内显著增加。柑橘类水果加工业的产量约为。全世界每年有1.1亿至1.2亿吨柑橘类水果废弃物。这反过来又造成垃圾填埋和环境污染,并对人类健康和生态系统构成威胁。柑橘废弃物的适当回收有助于减少污染,也可作为生产不同生物基产品的可持续来源。柑橘废弃物中丰富的生物活性化合物为生产各种有用产品提供了巨大的经济价值。此外,在柑橘废弃物中发现的生物活性化合物具有多种生物学特性,包括抗氧化、抗癌、抗诱变、抗血小板、心脏保护和抗病毒活性。柑橘废弃物不需要直接处理,而是可以被升级为各种增值产品,包括单细胞蛋白质、生物聚合物、果胶、生物燃料、生物肥料和生物能源。柑橘皮作为一种具有成本效益的营养保健品储存库,为退行性疾病的治疗提供了一种负担得起的饮食选择。柑橘废弃物被用作生物肥料,是酚类化合物和类胡萝卜素的丰富来源,有助于延长食物的保质期。其目的是在行业最新创新的帮助下保持经济可行性和可持续性。本文综述了柑橘类水果废弃物再生利用的最新进展,并介绍了提取柠檬烯、类黄酮和果胶等有价值的生物活性化合物的创新生物技术方法。这些化合物被用于不同的领域,从食品和制药工业到生物能源。微波辅助提取(MAE)和超声辅助提取(UAE)等技术具有产率高、节能等特点。研究了柑橘类水果废弃物的取样、预处理、植物化学物质提取、纯化和鉴定技术。此外,本综述强调了废物增值作为循环经济方法的一部分的环境效益,有助于经济可持续性和减少污染。
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引用次数: 0
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