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Optimization of Thermoultrasound Process of a Soursop (Annona muricata) Nectar and Its Comparison with Pasteurization on Physicochemical Properties and In Vitro Bioaccessibility of Antioxidants 酸浆(Annona muricata)花蜜热超声工艺的优化及其与巴氏杀菌法在抗氧化剂的理化性质和体外生物可及性方面的比较
IF 2.4 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-26 DOI: 10.17113/ftb.61.04.23.8180
Q. Zafra‐Rojas, José Luis Jiménez-Hernández, Enrique J. Olloqui, N. Cruz‐Cansino, Ernesto Alanís-García, Esther Ramírez-Moreno, J. A. Ariza-Ortega, Juan Carlos Moreno-Seceña
Research background. Soursop nectar contains antioxidants and for its conservation the pasteurization is applied. However, this technology decreases its physicochemical properties and bioactive compounds, hence, an alternative is the thermoultrasound which could counter these effects. Therefore, the thermoultrasonicated nectar was compared to a pasteurized one and the in vitro bioaccessibility of antioxidants was estimated. Experimental approach. The soursop nectar (25 %) was elaborated and the response surface methodology was used. The thermoultrasound (TUS) (75-90 % amplitude; 3.15-15 min) was applied, and 2 % stevia and 6 % of agave inulin were added as sweeteners. The microbiological, physicochemical, enzymatic and antioxidant properties were analyzed. The nectar was prepared again and control, optimized TUS and pasteurized were compared. In addition to the aforementioned determinations, microstructure, total dietary fiber (TDF) and in vitro bioaccessibility of antioxidants were performed. Results and conclusions. The response variables that fit the mathematical model were L*, b*, Chroma (C*), total phenolic content (TPC), antioxidant activity by ABTS•+, DPPH• and ferric reducing antioxidant power (FRAP). The L* and DPPH• were influenced by quadratic time and TPC by time (p<0.0001). The optimal TUS condition was 82 % amplitude at 9.15 min and the responses variables were L*, b* and C* (45.48, 3.55 and 3.62, respectively), TPC (38.40 mg GAE/100 mL), ABTS•+ (31.28 μmol TE/100 mL), DPPH• (124.22 μmol TE/100 mL) and FRAP (3.06 μmol Fe (II)/100 mL). In comparison with the pasteurized sample, optimized TUS had high value of TDF (3.53%), L* (45.56) h° (-56.49), TPC (26.63 mg GAE/100 mL), ABTS•+ and DPPH• (22.03 and 129.22 μmol TE/100 mL, respectively), FRAP (3.10 μmol Fe (II)/100 mL) and low pectin methylesterase activity (0.28 PMEU/mL). For in vitro bioaccessibility, the optimized TUS nectar showed high absorption of TPC (15.26 GAE/100 mL) and high values in the antioxidant activity by ABTS (34.92 μmol TE/100 mL) and FRAP (7.88 μmol Fe (II)/100 mL). Novelty and scientific contribution. The thermoultrasound improves the physicochemical properties and in vitro bioaccessibility of antioxidants soursop nectar. On the other hand, as an alternative, this beverage provides low-calorie with prebiotic properties for the benefit of consumer health.
研究背景。番荔枝花蜜含有抗氧化剂,为了保存这种物质,需要采用巴氏杀菌法。然而,这种技术会降低其理化特性和生物活性化合物,因此,热超声波是一种替代方法,可以抵消这些影响。因此,我们将经过热超声处理的花蜜与经过巴氏杀菌处理的花蜜进行了比较,并估算了抗氧化剂的体外生物可及性。实验方法。实验采用了响应面方法,对酸浆果花蜜(25%)进行了阐述。使用热超声波(TUS)(振幅 75-90%;3.15-15 分钟),并添加 2% 甜菊糖和 6% 龙舌兰菊粉作为甜味剂。对其微生物、理化、酶和抗氧化特性进行了分析。再次制备花蜜,比较对照组、优化 TUS 组和巴氏杀菌组。除上述测定外,还进行了微观结构、总膳食纤维(TDF)和抗氧化剂体外生物利用率的测定。结果和结论符合数学模型的响应变量有 L*、b*、色度(C*)、总酚含量(TPC)、ABTS-+、DPPH- 和铁还原抗氧化能力(FRAP)的抗氧化活性。L* 和 DPPH- 受二次时间影响,TPC 受时间影响(p<0.0001)。最佳 TUS 条件是在 9.15 分钟时振幅为 82%,响应变量为 L*、b* 和 C*(分别为 45.48、3.55 和 3.62)、TPC(38.40 mg GAE/100 mL)、ABTS-+(31.28 μmol TE/100 mL)、DPPH-(124.22 μmol TE/100 mL)和 FRAP(3.06 μmol Fe (II)/100 mL)。与巴氏杀菌样品相比,优化后的 TUS 具有较高的 TDF 值(3.53%)、L* (45.56) h° (-56.49)、TPC (26.63 mg GAE/100 mL)、ABTS-+ 和 DPPH- (分别为 22.03 和 129.22 μmol TE/100 mL)、FRAP (3.10 μmol Fe (II)/100 mL)和较低的果胶甲基酯酶活性 (0.28 PMEU/mL)。在体外生物可接受性方面,优化的 TUS 花蜜显示出较高的 TPC 吸收率(15.26 GAE/100 mL)和较高的 ABTS 抗氧化活性值(34.92 μmol TE/100 mL)和 FRAP 值(7.88 μmol Fe (II)/100 mL)。新颖性和科学贡献。热超声波提高了酸果树花蜜抗氧化剂的理化特性和体外生物可及性。另一方面,作为一种替代品,这种饮料具有低热量和益生元特性,有利于消费者的健康。
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引用次数: 0
Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik 总抗氧化潜能、总酚类概况和对脑癌的细胞毒活性:Melocan 和 Galdirik
IF 2.4 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-26 DOI: 10.17113/ftb.61.04.23.8071
Diaa Al Yassine, Nourhane El Massri, G. Demircan, Gülay Bulut, Demet Akin, Z. Tacer-Caba
Research background. Brain cancer is known to be one of the most difficult types of cancer to be cure. It has serious impact on the lives of diagnosed people due to the insufficient treatment options and their side effects. Thus, the search for novel alternative treatments is ongoing. Melocan (Smilax excelsa L.) and Galdirik (Trachystemon orientalis) are significant contributors to both traditional culinary culture and traditional medicine around Black Sea; however, the evidence regarding their antioxidative and cytotoxic effects remains fairly limited. Experimental approach. This study aimed to determine the antioxidant and cytotoxic activity of Smilax excelsa and Trachystemon orientalis, on the C6 glioblastoma cell line. Smilax excelsa and Trachystemon orientalis plants were dried and extracted; then, the total phenolic content (TPC) and their phenolic profiles were studied. In addition, their Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) were determined by using an assay kit. We also analyzed the total antioxidant activity (TAA) using the DPPH radical scavenging assay and the cytotoxic impact on the glioma cells via the 3-(4,5-dimethylthiazol-2 yl)-2,5-diphenyltetrazolium (MTT) assay. Results and conclusions. According to the results, the water extracts of both Smilax excelsa (1158.17 mg gallic acid eq./100 g DM) and Trachystemon orientalis (262 mg gallic acid eq./0 g DM) had higher TPC than the ethanol extracts. TAA were measured as 192.86 mg and 131.92 Trolox eq./100 g DM for the Smilax excels and Trachystemon orientalis, respectively. The MTT assay revealed that Trachystemon orientalis had a greater cytotoxic effect. In conclusion, the findings of the current study are promising. Novelty and scientific contribution. This is the first study that aimed to evaluate the potential cytotoxic activity of two local Turkish plants, Smilax excelsa and Trachystemon orientalis, against C6 glioblastoma cells and the findings ensure that both plants may be used in the future as good therapeutic agents for treating cancer.
研究背景。众所周知,脑癌是最难治愈的癌症类型之一。由于治疗方案不足及其副作用,它严重影响了确诊患者的生活。因此,人们一直在寻找新的替代治疗方法。Melocan (Smilax excelsa L.)和 Galdirik (Trachystemon orientalis)是黑海地区传统烹饪文化和传统医学的重要组成部分;然而,有关其抗氧化和细胞毒性作用的证据仍然相当有限。实验方法。本研究旨在确定菝葜和东方桔梗对 C6 胶质母细胞瘤细胞系的抗氧化和细胞毒性活性。研究人员首先对Smilax excelsa和Trachystemon orientalis植物进行干燥和提取,然后研究了它们的总酚含量(TPC)和酚谱。此外,还使用检测试剂盒测定了它们的总抗氧化状态(TAS)和总氧化状态(TOS)。我们还利用 DPPH 自由基清除试验分析了总抗氧化活性(TAA),并通过 3-(4,5-二甲基噻唑-2 yl)-2,5-二苯基四唑(MTT)试验分析了对胶质瘤细胞的细胞毒性影响。 结果和结论。结果表明,Smilax excelsa 水提取物(1158.17 毫克没食子酸当量/100 克 DM)和 Trachystemon orientalis 水提取物(262 毫克没食子酸当量/0 克 DM)的 TPC 均高于乙醇提取物。经测定,Smilax excels 和 Trachystemon orientalis 的 TAA 分别为 192.86 mg 和 131.92 Trolox eq./100 g DM。MTT 试验表明,东方桔梗的细胞毒性作用更大。总之,目前的研究结果很有希望。新颖性和科学贡献。这是第一项旨在评估两种土耳其本地植物(Smilax excelsa 和 Trachystemon orientalis)对 C6 胶质母细胞瘤细胞的潜在细胞毒性活性的研究。
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引用次数: 0
Chemical Characterization and Antioxidant Potential of the Rowan Fruits (Sorbus aucuparia L.) from Alpine-Dinaric region of Croatia 克罗埃西亚阿尔卑斯-迪纳尔地区棘豆果实(Sorbus aucuparia L.)的化学特性及抗氧化潜力
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.61.04.23.8225
Antonija Sulimanec, Karla Kragić, Ankica Sekovanić, Jasna Jurasović, Ines Panjkota Krbavčić, Nada Vahčić, Antonio Vidaković, Igor Poljak, Ivana Rumora Samarin
Research background. The rowan (Sorbus aucuparia L.) is a small tree in the Rosaceae family with characteristic orange-red fruits. Raw fruits can be used for making jams, juices, and puree, as well as dried ones for teas. In folk medicine, they were used to prevent scurvy and bleeding or as a diuretic and laxative. The aim of this study was to characterize the proximate chemical composition, antioxidant potential, and macro and trace elements of the rowan fruits for their potential use as a functional food. Experimental approach. Fruits were collected in the Alpine-Dinaric region of Croatia in 12 populations. After collection, samples were transported to the laboratory, chopped into small pieces, placed in plastic containers, and stored at -20 ºC until analysis. Proximate chemical composition, including ash, water, cellulose, crude fat, crude protein, was determined according to the official AOAC methods and total carbohydrates as non-structural carbohydrates. Total phenolic content (TPC) and antioxidative capacity (TAC) were also measured. For multielement analysis, fruits were cleaned from the dust, lyophilized, homogenized, and acid digested in a microwave system. Concentrations of elements were quantified using the ICP-MS method. Results and conclusions. The basic constituents in analyzed fruits were 76.53 % of water, 17.45 % of total carbohydrates, 2.98 % of crude proteins, 1.49 % of crude fats, 1.07 % of cellulose, and 1.29 % of ash. On average, the TPC was 932 mg/100 g and the TAC was (60.14±14.48) % and (4.1±1.2) mmol Fe2+/100 g, determined by DPPH and FRAP assay, respectively. Levels of elements decreased as follows (in mg/kg): K: 2485 > Ca: 459 > P: 206, Mg: 193 > Na: 6.29 > Fe: 3.68, Mn: 3.58 > Zn: 1.11 > Cu: 0.731 > Mo: 0.098 > Co: 0.003 > Se: 0.001. In comparison with the literature, the phenolic and element content of the rowan fruits are similar to that of blueberry and raspberry. Obtained results suggest that the rowan fruits have valuable nutritional properties and could be useful for the fortification in the food industry. Novelty and scientific contribution. The importance of the obtained results is reflected in the completion of the literature gaps on the elemental composition, especially on the content of essential macro- and trace elements, as well as the antioxidant potential of rowan fruits.
研究背景。蔷薇(Sorbus aucuparia L.)是蔷薇科的一种小树,果实呈橘红色。生水果可以用来做果酱、果汁和果泥,也可以用来做茶。在民间医学中,它们被用来预防坏血病和出血,或作为利尿剂和泻药。本研究的目的是表征rowan果实的近似化学成分、抗氧化潜力、宏量元素和微量元素,为其作为功能食品的潜在用途。实验方法。在克罗地亚的阿尔卑斯-迪纳尔地区收集了12个种群的水果。收集后,将样品运送到实验室,切成小块,放入塑料容器中,在-20ºC保存直至分析。近似化学成分包括灰分、水、纤维素、粗脂肪、粗蛋白质,按官方AOAC方法测定,总碳水化合物为非结构性碳水化合物。测定了总酚含量(TPC)和抗氧化能力(TAC)。为了进行多元素分析,将水果从灰尘中清除,冻干,均质,并在微波系统中酸消化。采用ICP-MS法测定元素浓度。结果和结论。果实的基本成分为76.53%的水分、17.45%的碳水化合物、2.98%的粗蛋白质、1.49%的粗脂肪、1.07%的纤维素和1.29%的灰分。DPPH法和FRAP法测定的TPC平均值为932 mg/100 g, TAC平均值为(60.14±14.48)%和(4.1±1.2)mmol Fe2+/100 g。元素含量下降如下(单位:mg/kg): K: 2485 >Ca: 459 >P: 206, Mg: 193 >Na: 6.29 >Fe: 3.68, Mn: 3.58 >Zn: 1.11 >Cu: 0.731 >Mo: 0.098 >Co: 0.003 >Se: 0.001。与文献比较,rowan果实的酚类和元素含量与蓝莓和覆盆子相似。研究结果表明,罗文果实具有丰富的营养价值,可用于食品工业的营养强化。创新和科学贡献。所获得的结果的重要性体现在完成了文献空白的元素组成,特别是必需的宏量元素和微量元素的含量,以及rowan果实的抗氧化潜力。
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引用次数: 0
Milk-Clotting Properties and Primary Proteolysis of a Lyophilised Extract from Artichoke Flowers (Cynara scolymus L.) 洋蓟花(Cynara scolymus L.)冻干提取物的凝乳特性和初级蛋白水解
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.61.03.23.8142
Valentina Crosetti, Agustín Sola, Gabriela Grigioni, María José Torres
Research background. A few studies have investigated Cynara scolymus enzymes as a substitute for calf rennet in cheese making. They used aqueous extracts prepared by maceration of plant material and stored by freezing. However, it was indicated that lyophilisation is a better alternative to preserve the coagulant properties of plant extracts over a longer period of time, as it is a more controllable, stable and hygienic alternative with a better shelf life that is easier to transport, store and standardise. Experimental approach. We obtained a lyophilised extract of mature artichoke flowers, named CS, which was characterised for its milk-clotting properties at different pH and temperatures. In addition, the potential yield, whey composition and the primary hydrolysis profile of caseins by urea-polyacrylamide gel electrophoresis (PAGE) of mini curds prepared with different doses of coagulant were determined. Results and conclusions. The lyophilised extract was able to hydrolyse casein and showed stable proteolytic activity at pH=6.4 and 37 °C for 50 min, which decreased when the process temperature was increased to 41 and 45 °C and was lost at 70 °C. On the other hand, milk-clotting activity increased significantly (p<0.001) when the temperature increased from 37 to 45 °C and the pH of the milk decreased from 6.8 to 5.8. Potential yield between 10 and 17 % was obtained for the mini curds prepared with the lyophilised artichoke extract, and the casein degradation pattern obtained by urea-PAGE was similar to that of the commercial coagulant. Novelty and scientific contribution. On a laboratory scale, our work has shown that the lyophilised artichoke extract has sufficient proteolytic and coagulant activity to be used as a milk coagulant, i.e. plant rennet, in cheese making as an alternative to animal rennet. As this extract is lyophilised, it has the advantage of being a better alternative in terms of preservation and shelf-life. It offers an innovative way to diversify cheese products and appeal to consumers with different dietary preferences and needs.
研究背景。一些研究已经研究了猪舌草酶作为小牛凝乳酶在奶酪制作中的替代品。他们使用浸渍植物材料制备的水提取物,并通过冷冻储存。然而,研究表明,冻干是一种更好的替代方法,可以在更长的时间内保持植物提取物的凝固特性,因为它是一种更可控、稳定和卫生的替代方法,具有更好的保质期,更容易运输、储存和标准化。实验方法。我们获得了成熟洋蓟花的冻干提取物,命名为CS,其特点是在不同的pH值和温度下具有凝乳特性。此外,通过尿素-聚丙烯酰胺凝胶电泳(PAGE)测定了不同剂量混凝剂制备的微型凝乳的潜在产率、乳清成分和酪蛋白的初级水解谱。结果和结论。冻干提取物在pH=6.4和37℃条件下水解酪蛋白50 min,具有稳定的蛋白水解活性,当工艺温度升高到41℃和45℃时,蛋白水解活性下降,70℃时完全丧失。另一方面,当温度从37°C增加到45°C,牛奶的pH从6.8降低到5.8时,凝乳活性显著增加(p<0.001)。用冻干洋蓟提取物制备的迷你凝乳的潜在产率在10%到17%之间,尿素- page法获得的酪蛋白降解模式与商业混凝剂相似。创新和科学贡献。在实验室规模上,我们的工作表明,冷冻干燥的朝鲜蓟提取物具有足够的蛋白质水解和凝固活性,可以用作牛奶凝固剂,即植物凝乳酶,在奶酪制作中作为动物凝乳酶的替代品。由于这种提取物是冻干的,因此在保存和保质期方面具有更好的替代优势。它提供了一种创新的方式,使奶酪产品多样化,并吸引不同饮食偏好和需求的消费者。
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引用次数: 0
Corrigendum to: Fruit Extract Derived from a Mixture of Noni, Pineapple and Mango Capable of Coagulating Milk and Producing Curd with Antidiabetic Activities (Published: Food Technol. Biotechnol. 60 (3) 375-385 (2022) https://doi.org/10.17113/ftb.60.03.22.7456) 从诺丽、菠萝和芒果混合物中提取的能够凝固牛奶并产生具有抗糖尿病活性凝乳的水果提取物的勘误(出版:食品技术。生物技术。60 (3)375-385 (2022)https://doi.org/10.17113/ftb.60.03.22.7456)
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.60.03.22.7456_corrig.61(3)
Jaya Vejayan, Rupbansraaj Bathmanathan, Sharifah Aminah Tuan Said, Srikumar Chakravarthi, Halijah Ibrahim
The authors request that the Funding section be amended to conform to the correct format required by the funding institution, i.e. the Ministry of Higher Education of Malaysia. Previously written statement in the funding section: Funding for this work was provided by Ministry of Education, Malaysia, with Fundamental Research Grant Scheme (FRGS) with external reference number FRGS/1/2022/STG01/ UMP/02/1 and title: Investigations of Protein from the Lesser Known Tongkat Ali Plants of Stema tuberosa and Polyalthia bullata for Their Potentials in Improving Men’s Health. The present study was the outcome of using chemicals and consumables purchased from this grant without compromising its main objectives and milestones. is changed to: The authors would like to thank Ministry of Higher Education for providing financial support under Fundamental Research Grant Scheme (FRGS) No: FRGS/1/2022/STG01/UMP/02/1 (University reference RDU220110) and Universiti Malaysia Pahang for laboratory facilities. The title of the FRGS grant: Investigations of Protein from the Lesser Known Tongkat Ali Plants of Stema tuberosa and Polyalthia bullata for Their Potentials in Improving Men’s Health. The present study was the outcome of using chemicals and consumables purchased from this grant without compromising its main objectives and milestones.
作者要求修改资助部分,以符合资助机构(即马来西亚高等教育部)所要求的正确格式。资助部分先前的书面声明:这项工作的资金由马来西亚教育部提供,基础研究资助计划(FRGS),外部参考文献号为FRGS/1/2022/STG01/ UMP/02/1,标题为:从鲜为人知的东吉阿里植物Stema tuberosa和Polyalthia bullata中研究蛋白质对改善男性健康的潜力。本研究是在不影响其主要目标和里程碑的情况下使用从这笔赠款购买的化学品和消耗品的结果。作者要感谢马来西亚高等教育部根据基础研究资助计划(FRGS)提供的财政支持,编号:FRGS/1/2022/STG01/UMP/02/1(大学参考编号RDU220110)和马来西亚彭杭大学提供的实验室设施。FRGS资助的标题:从不太知名的东革阿里植物茎和蓼中提取蛋白质,研究它们改善男性健康的潜力。本研究是在不影响其主要目标和里程碑的情况下使用从这笔赠款购买的化学品和消耗品的结果。
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引用次数: 0
How to Increase the Nutritional Quality of Stinging Nettle Through Controlled Plant Nutrition 如何通过控制植物营养提高刺荨麻的营养品质
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.61.04.23.8119
Mia Dujmović, Nevena Opačić, Sanja Radman, Sanja Fabek Uher, Lepomir Čoga, Marko Petek, Sandra Voća Sandra Voća, Jana Šic Žlabur
Research background. As food production faces major challenges, modern agricultural practices are increasingly focused on conserving resources, reducing negative environmental impacts, and sustainably producing foods high in health-promoting phytochemicals. During the production process, many factors can influence the quality and chemical composition of a final food product. Proper selection of cultivating conditions, especially a balanced nutrition, can significantly increase nutritional value resulting in foods with strong biological and functional properties. Stinging nettle is a rich source of minerals, vitamins, pigments, phenols and other bioactive compounds (BC) and can be consumed as a green leafy vegetable with beneficial effects on human health. Therefore, the aim of this study was to determine the nutritional quality and antioxidant capacity of stinging nettle leaves under the influence of different nutrient solution (NS) treatments during three mowings. Experimental approach. The experiment was performed in a floating hydroponic system, with different treatments of NS application during three mowings. After each mowing, the following treatments were differed: treatment 1 – depletion with water, treatment 2 - supplementation with standard NS, and treatment 3 - correction with nutrients. Of the BC minerals, ascorbic acid, phenols, and photosynthetic pigments content, as well as antioxidant capacity were analyzed spectrophotometrically, while individual phenols were determined by liquid chromatography. Results and conclusions. Different nutrition solution treatments and the number of mowings had a significant influence on the content of the analyzed BC. The highest contents of total phenols (377.04 mg GAE/100 g fm), total flavonoids (279.54 mg CTH/100 g fm), ascorbic acid (112.37 mg/100 g fm), and pigments (total chlorophylls 1.84, and total carotenoids 0.36 mg/g), as well as the highest antioxidant capacity (35.47 µmol TE/g) were recorded in the third mowing, with nutrient solution supplementation. Novelty and scientific contribution. This is the first time that stinging nettle leaves are produced in a floating hydroponic system by controlled plant nutrition. We establish this type of nutrition manipulation during multiple mowings as an innovative technique for the production of novel food with high and improved nutritional value suitable for consumption as green leafy vegetables.
研究背景。由于粮食生产面临重大挑战,现代农业实践越来越注重节约资源,减少对环境的负面影响,并可持续地生产富含促进健康的植物化学物质的食物。在生产过程中,许多因素会影响最终食品的质量和化学成分。适当选择栽培条件,特别是均衡的营养,可以显著提高营养价值,从而使食品具有较强的生物学和功能特性。荨麻是矿物质、维生素、色素、酚类和其他生物活性化合物(BC)的丰富来源,可作为绿叶蔬菜食用,对人体健康有益。因此,本研究旨在研究不同营养液处理对刺荨麻叶片营养品质和抗氧化能力的影响。实验方法。试验在浮式水培系统中进行,在三次刈割中施用不同的NS处理。每次刈割后,采取不同的处理方式:处理1 -耗水,处理2 -补充标准氮肥,处理3 -补养。用分光光度法测定BC矿物的抗坏血酸、酚类、光合色素含量及抗氧化能力,用液相色谱法测定各酚类。结果和结论。不同营养液处理和刈割次数对所分析的BC含量有显著影响。第三次割草中总酚含量最高(377.04 mg GAE/100 g fm),总黄酮含量最高(279.54 mg CTH/100 g fm),抗坏血酸含量最高(112.37 mg/100 g fm),色素含量最高(总叶绿素1.84,总类胡萝卜素0.36 mg/g),抗氧化能力最高(35.47µmol TE/g)。创新和科学贡献。这是第一次在浮动水培系统中通过控制植物营养生产荨麻叶子。我们建立了这种在多次刈割过程中的营养操纵,作为一种创新技术,用于生产具有高营养价值和改善营养价值的新型食品,适合作为绿叶蔬菜食用。
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引用次数: 0
Corrigendum to: Impact of Successive Exploitation of a Saccharomyces pastorianus Starter Culture on Saccharide Uptake Dynamics from Wort (Published: Food Technol. Biotechnol. 59 (1) 16-23 (2021) https://doi.org/10.17113/ftb.59.01.21.6899) 连续开发一种酵母发酵剂对麦汁中糖类摄取动态的影响的更正(已出版:食品技术。生物技术。59 (1)16-23 (2021)https://doi.org/10.17113/ftb.59.01.21.6899)
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.59.01.21.6899_corrig.61(3)
Miha Ocvirk, Nataša Mlinarič, Iztok Jože Košir
The authors would like to state that they inadvertently omitted one of the authors when preparing the manuscript. The authors of this work are as follows: Miha Ocvirk1, Nataša Kočar Mlinarič2, Peter Raspor3 and Iztok Jože Košir1* 1Slovenian Institute of Hop Research and Brewing, C. Žalskega tabora 2, 3310 Žalec, Slovenia 2Pivovarna Laško Union d.o.o., Pivovarniška ulica 2, 1000 Ljubljana, Slovenia 3Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
作者在此声明,他们在准备稿件时不慎遗漏了其中一位作者。本作品的作者如下Miha Ocvirk1、Nataša Kočar Mlinarič2、Peter Raspor3 和 Iztok Jože Košir1* 1Slovenian Institute of Hop Research and Brewing, C. Žalskega tabora 2, 3310 Žalec, Slovenia 2Pivovarna Laško Union d.o.o.、Pivovarniška ulica 2, 1000 Ljubljana, Slovenia 3Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
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引用次数: 0
Extraction of Heat Stabilised Defatted Rice Bran Protein by Solid-State Fermentation Using Heterofermentative Microbes from Asian Traditional Starters 亚洲传统发酵剂中热稳定脱脂米糠蛋白的固态发酵
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-01 DOI: 10.17113/ftb.61.04.23.8255
Ugyen Ugyen, Raintong Singanusong, Mahattanee Phinyo, Phanupong Changtor, Sirilux Chaijamrus, Tipawan Thongsook
Research background. Heat stabilised defatted rice bran (HSDRB) is a primary by-product of rice bran oil extraction industries and a nutritious protein source. However, despite the unique nutritional profile of RB protein, the protein-rich by-product, HSDRB is underutilized as low-value animal feed. Research on protein extraction from HSDRB via enzymatic hydrolysis has gained the attention of numerous scholars. However, a cost-effective extraction method is required to mitigate the high expenses associated with enzymes. Therefore, we presented an alternative economical and natural approach for HSDRB protein extraction using solid-state fermentation (SSF) with heterofermentative microbes. Experimental approach. SSF of HSDRB with two kinds of Asian traditional fermentative starters, namely Loog-pang and Koji, were investigated for enzyme production and their efficacy in extracting protein from HSDRB. For this purpose, HSDRB fermentation for 0, 12, 24, 48, 72 and 96 h followed by 24 h hydrolysis was carried out to evaluate the extracted RB protein. Moreover, the microbiome diversity in the fermentative starters was also determined by metagenomic sequencing of 16S rRNA and internal transcribed spacer for bacterial and fungal identification, respectively. Results and conclusions. The microbial community in the fermentative starters revealed the dominance of lactic acid bacteria (LAB) such as Bacillus subtilis in Loog-pang and Streptococcus lutetiensis, Bacillus pumilus, Lactococcus cremoris, Lactococcus garvieae, and Pediococcus pentosaceus in Koji whereas yeast species, Saccharomycopsis fibuligera, and Saccharomyces cerevisiae were dominating the fungal diversity in Loog-pang and Koji starters, respectively. Results suggest that Loog-pang and Koji could produce cellulase, neutral, and acid proteases during fermentation. Despite their microbial diversity discrepancy and the enzyme activity during SSF, both starters were effective in enhancing protein extraction from HSDRB. A positive relationship between the SSF duration and extracted protein was noted. SSF by Loog-pang and Koji after 72 h followed by 24 h hydrolysis was found to extract 65.66 and 66.67 % protein from HSDRB, respectively. The amino acids profile of the protein hydrolysate prepared by non-fermented and fermented method showed no difference and displayed an abundance of glutamic acid, aspartic acids, leucine, arginine, alanine, and glycine amino acids, accounted for approximately 58 % of the total amino acids. Novelty and scientific contribution. Loog-pang and Koji (Thai and Japanese traditional fermentative starters, respectively), were found effective in extracting protein from HSDRB by SSF despite being inexpensive microbial enzyme sources, and future research aimed at scaling up HSDRB protein extraction for usage in industrial applications can rely on our findings.
研究背景。热稳定脱脂米糠(HSDRB)是米糠榨油工业的主要副产品,也是一种营养丰富的蛋白质来源。然而,尽管富含蛋白质的副产品RB蛋白具有独特的营养成分,但HSDRB作为低价值动物饲料的利用不足。酶法从HSDRB中提取蛋白质的研究得到了众多学者的关注。然而,需要一种具有成本效益的提取方法来降低与酶相关的高昂费用。因此,我们提出了一种经济、自然的提取HSDRB蛋白的方法,即利用异源微生物进行固态发酵(SSF)。实验方法。以两种亚洲传统发酵剂——龙丸和曲曲为发酵剂,对HSDRB的SSF产酶及对HSDRB蛋白的提取效果进行了研究。为此,采用HSDRB发酵0、12、24、48、72和96 h,水解24 h,对提取的RB蛋白进行评价。此外,还通过16S rRNA和内部转录间隔序列的宏基因组测序分别确定了发酵发酵剂中微生物组的多样性,用于细菌和真菌鉴定。结果和结论。发酵发酵剂中的微生物群落多样性以枯草芽孢杆菌(Bacillus subtilis)和黄体链球菌(Streptococcus lutetiensis)、矮秆芽孢杆菌(Bacillus pumilus)、乳酸菌球菌(Lactococcus cremoris)、garvieae乳球菌(Lactococcus garvieae)和五香球球菌(Pediococcus pentosaceus)等乳酸菌(LAB)为主,而酵母菌(yeast species)、纤维酵母菌(Saccharomycopsis fibuligera)和酿酒酵母菌(Saccharomyces cerevisiae)在发酵发酵剂中分别占主导地位。结果表明,龙肚和曲在发酵过程中可产生纤维素酶、中性蛋白酶和酸性蛋白酶。尽管两种发酵剂在SSF过程中微生物多样性和酶活性存在差异,但它们都能有效地提高HSDRB的蛋白质提取率。SSF持续时间与提取蛋白呈显著正相关。经72 h和24 h水解后,经loong -pang和Koji的SSF, HSDRB蛋白提取率分别为65.66%和66.67%。非发酵法和发酵法制备的蛋白水解液的氨基酸谱没有差异,显示出丰富的谷氨酸、天冬氨酸、亮氨酸、精氨酸、丙氨酸和甘氨酸氨基酸,约占总氨基酸的58%。创新和科学贡献。尽管作为廉价的微生物酶源,Loog-pang和Koji(分别为泰国和日本的传统发酵发酵剂)被发现可以有效地从HSDRB中提取蛋白质,未来旨在扩大HSDRB蛋白质提取用于工业应用的研究可以依赖于我们的发现。
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引用次数: 0
The Impact of Nutrition on the Onset, Course of the Disease and Quality of Life of Patients with Laryngopharyngeal Reflux 营养对咽喉反流患者发病、病程及生活质量的影响
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-10-22 DOI: 10.17113/ftb.61.04.23.8222
Tin Prpić, Melita Peček Prpić, Tihana Mendeš, Anamarija Šestak, Andrijana Včeva
Research background. The role of dietary habits in patients with laryngopharyngeal reflux disease (LPR) is comparatively underexplored. The aim of the study is to examine dietary habits, onset and course of the disease as well as the quality of life of patients with LPR. Experimental approach. The results of the modified food frequency questionnaire (FFQ-m) and Laryngopharyngeal Reflux Health-Related Quality of Life (LPR-HRQL) questionnaires were compared between subjects with and without LPR. There were a total of 100 subjects with LPR and 65 subjects in the control group. The group of subjects with LPR was further randomly divided into two subgroups; the first subgroup was treated with esomeprazole in a twice daily dose of 20 mg combined with dietary and general lifestyle changes instructions, and the other with pantoprazole in a twice-daily dose of 20 mg combined with dietary and general lifestyle changes instructions. Participants were instructed to fill out FFQ-m and LPR-HRQL questionnaires immediately after the initial examination and then after control examinations that were 30 and 60 days after the initial examination. Results and conclusions. Patients with LPR consume more food with high reflux potential, drink more carbonated drinks and juices, and have a worse quality of life compared to the control group (p<0.001). Proton pump inhibitors in a twice-daily dose of 20 mg with a change in dietary habits, such as avoiding acidic, spicy, fermented, sweet, and fried foods same as other foods with a high reflux potential as well as carbonated drinks and juices with the introduction of foods with a low reflux potential and water significantly reduces symptoms of LPR and increases the quality of life (p<0.001). Novelty and scientific contribution. This is the first study showing the connection between dietary habits and quality of life in patients with LPR. The contribution of this research is an objective review of the follow-up of patients with LPR that could be used in their regular assessment.
研究背景。饮食习惯在喉咽反流病(LPR)患者中的作用尚未得到充分探讨。该研究的目的是检查LPR患者的饮食习惯、发病和病程以及生活质量。实验方法。将改良食物频率问卷(FFQ-m)和咽喉反流健康相关生活质量问卷(LPR- hrql)的结果在有和没有LPR的受试者之间进行比较。LPR组共100例,对照组65例。LPR组进一步随机分为2个亚组;第一组患者接受埃索美拉唑治疗,每日两次剂量20mg,并结合饮食和一般生活方式改变指导;另一组患者接受泮托拉唑治疗,每日两次剂量20mg,并结合饮食和一般生活方式改变指导。参与者被要求在初次检查后立即填写FFQ-m和LPR-HRQL问卷,然后在初次检查后30天和60天的对照检查后填写。结果和结论。与对照组相比,LPR患者消耗更多具有高反流潜力的食物,饮用更多碳酸饮料和果汁,生活质量更差(p<0.001)。质子泵抑制剂每日服用两次,剂量为20毫克,同时改变饮食习惯,如避免食用酸性、辛辣、发酵、甜味和油炸食品以及其他具有高反流潜力的食物,以及碳酸饮料和果汁,同时引入具有低反流潜力的食物和水,可显著减轻LPR的症状并提高生活质量(p<0.001)。创新和科学贡献。这是第一个显示LPR患者饮食习惯和生活质量之间联系的研究。本研究的贡献是对LPR患者随访的客观回顾,可用于其定期评估。
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引用次数: 0
Optimizing the Production Process of Bacterial Nanocellulose: Impact on Growth and Bioactive Compounds 优化细菌纳米纤维素的生产工艺:对生长和生物活性化合物的影响
4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-10-22 DOI: 10.17113/ftb.61.04.23.8182
Nicole Folmann Lima, Giselle Maria Maciel, Isabela de Andrade Arruda Fernandes, Charles Windson Isidoro Haminiuk
Research background. The field of research on bacterial cellulose production has been growing rapidly in recent years, with the potential for its use in various applications, such as in the medical and food industries. Previous studies have focused on optimizing the production process through various methods, such as using different carbon sources and manipulating environmental conditions. However, further research is still needed to optimise the production process and understand the underlying mechanisms of bacterial cellulose synthesis. Experimental approach. We have used Plackett-Burman and Box-Behnken experimental designs to analyse various factors impact on bacterial cellulose production. The optimized medium was analysed for fermentation kinetics, and the cellulose produced was characterised. This approach was used because it allows for the identification of significant factors impacting bacterial cellulose growth, the optimisation of the culture medium, and the characterisation of the produced cellulose. Results and conclusions. The results indicated that higher sucrose concentrations, higher kombucha levels, and lower symbiotic culture of bacteria and yeast size were the most significant factors for improving bacterial cellulose production, while the others had no relevant impact. The optimized medium showed an increase in the concentration of total phenolic compounds and total flavonoids, as well as relevant levels of antioxidant activity. The pure bacterial cellulose produced showed high water absorption capacity, in addition to high crystallinity and thermal stability. Novelty and scientific contribution. The study makes a significant scientific contribution by optimizing the culture medium to produce bacterial cellulose in a more productive and efficient way. The optimized medium can be used for producing a kombucha-type drink containing a high content of bioactive compounds and the production of bacterial cellulose with high crystallinity and thermal stability. Additionally, the study highlights the potential of bacterial cellulose as a highly water-absorbing material with applications in areas such as packaging and biomedical engineering.
研究背景。近年来,关于细菌纤维素生产的研究领域发展迅速,在医疗和食品工业等各种应用领域具有潜在的用途。以前的研究主要集中在通过各种方法优化生产过程,例如使用不同的碳源和操纵环境条件。然而,仍需要进一步的研究来优化生产工艺并了解细菌纤维素合成的潜在机制。实验方法。我们使用Plackett-Burman和Box-Behnken实验设计来分析影响细菌纤维素生产的各种因素。对优化后的培养基进行了发酵动力学分析,并对所产纤维素进行了表征。之所以使用这种方法,是因为它允许识别影响细菌纤维素生长的重要因素,培养基的优化,以及所产生的纤维素的表征。结果和结论。结果表明,较高的蔗糖浓度、较高的康普茶水平、较低的细菌共生培养量和酵母大小是提高细菌纤维素产量的最显著因素,而其他因素对细菌纤维素产量无显著影响。优化后的培养基中总酚类化合物和总黄酮含量显著提高,抗氧化活性也相应提高。制备的纯细菌纤维素除结晶度高、热稳定性好外,还具有较高的吸水能力。创新和科学贡献。该研究通过优化培养基,使细菌纤维素的生产效率更高,具有重要的科学贡献。该优化培养基可用于生产含有高含量生物活性化合物的红茶菌型饮料和生产具有高结晶度和热稳定性的细菌纤维素。此外,该研究还强调了细菌纤维素作为一种高度吸水材料在包装和生物医学工程等领域的应用潜力。
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引用次数: 0
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Food Technology and Biotechnology
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