首页 > 最新文献

Frontiers in Cardiovascular Medicine最新文献

英文 中文
Recommendations for prediction models in clinical practice guidelines for cardiovascular diseases are over-optimistic: a global survey utilizing a systematic literature search. 心血管疾病临床实践指南对预测模型的建议过于乐观:一项利用系统文献检索进行的全球调查。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1449058
Cheng-Yang Jing, Le Zhang, Lin Feng, Jia-Chen Li, Li-Rong Liang, Jing Hu, Xing Liao

Background: This study aimed to synthesize the recommendations for prediction models in cardiovascular clinical practice guidelines (CPGs) and assess the methodological quality of the relevant primary modeling studies.

Methods: We performed a systematic literature search of all available cardiovascular CPGs published between 2018 and 2023 that presented specific recommendations (whether in support or non-support) for at least one multivariable clinical prediction model. For the guideline-recommended models, the assessment of the methodological quality of their primary modeling studies was conducted using the Prediction model Risk Of Bias ASsessment Tool (PROBAST).

Results: In total, 46 qualified cardiovascular CPGs were included, with 69 prediction models and 80 specific recommendations. Of the 80 specific recommendations, 74 supported 57 models (53 were fully recommended and 4 were conditionally recommended) in cardiovascular practice with moderate to strong strength. Most of the guideline-recommended models were focused on predicting prognosis outcomes (53/57, 93%) in primary and tertiary prevention, focusing primarily on long-term risk stratification and prognosis management. A total of 10 conditions and 7 types of target population were involved in the 57 models, while heart failure (14/57, 25%) and a general population with or without cardiovascular risk factor(s) (12/57, 21%) received the most attention from the guidelines. The assessment of the methodological quality of 57 primary studies on the development of the guideline-recommended models revealed that only 40% of the modeling studies had a low risk of bias (ROB). The causes of high ROB were mainly in the analysis and participant domains.

Conclusions: Global cardiovascular CPGs presented an unduly positive appraisal of the existing prediction models in terms of ROB, leading to stronger recommendations than were warranted. Future cardiovascular practice may benefit from well-established clinical prediction models with better methodological quality and extensive external validation.

背景:本研究旨在综合心血管临床实践指南(CPG)中关于预测模型的建议,并评估相关主要建模研究的方法学质量:本研究旨在综合心血管临床实践指南(CPG)中对预测模型的建议,并评估相关主要建模研究的方法学质量:我们对2018年至2023年间发布的所有现有心血管CPG进行了系统性文献检索,这些CPG对至少一种多变量临床预测模型提出了具体建议(无论是支持还是不支持)。对于指南推荐的模型,使用预测模型偏倚风险评估工具(PROBAST)对其主要建模研究的方法学质量进行了评估:结果:共纳入 46 项合格的心血管 CPG,其中包括 69 个预测模型和 80 项具体建议。在 80 项具体建议中,74 项支持 57 个模型(53 项完全推荐,4 项有条件推荐)在心血管实践中的应用,支持力度为中等到较强。大多数指南推荐的模型都侧重于预测一级和三级预防的预后结果(53/57,93%),主要侧重于长期风险分层和预后管理。57 个模型共涉及 10 种疾病和 7 类目标人群,而心力衰竭(14/57,25%)和有或无心血管风险因素的普通人群(12/57,21%)受到指南的关注最多。对 57 项关于指南推荐模型开发的主要研究进行方法学质量评估后发现,只有 40% 的建模研究存在较低的偏倚风险(ROB)。造成高偏倚风险的原因主要在于分析和参与者领域:结论:全球心血管健康指导原则对现有预测模型的偏倚风险做出了过于积极的评价,从而导致推荐结果超出了合理范围。未来的心血管实践可能会受益于方法学质量更高、外部验证更广泛的成熟临床预测模型。
{"title":"Recommendations for prediction models in clinical practice guidelines for cardiovascular diseases are over-optimistic: a global survey utilizing a systematic literature search.","authors":"Cheng-Yang Jing, Le Zhang, Lin Feng, Jia-Chen Li, Li-Rong Liang, Jing Hu, Xing Liao","doi":"10.3389/fcvm.2024.1449058","DOIUrl":"10.3389/fcvm.2024.1449058","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to synthesize the recommendations for prediction models in cardiovascular clinical practice guidelines (CPGs) and assess the methodological quality of the relevant primary modeling studies.</p><p><strong>Methods: </strong>We performed a systematic literature search of all available cardiovascular CPGs published between 2018 and 2023 that presented specific recommendations (whether in support or non-support) for at least one multivariable clinical prediction model. For the guideline-recommended models, the assessment of the methodological quality of their primary modeling studies was conducted using the Prediction model Risk Of Bias ASsessment Tool (PROBAST).</p><p><strong>Results: </strong>In total, 46 qualified cardiovascular CPGs were included, with 69 prediction models and 80 specific recommendations. Of the 80 specific recommendations, 74 supported 57 models (53 were fully recommended and 4 were conditionally recommended) in cardiovascular practice with moderate to strong strength. Most of the guideline-recommended models were focused on predicting prognosis outcomes (53/57, 93%) in primary and tertiary prevention, focusing primarily on long-term risk stratification and prognosis management. A total of 10 conditions and 7 types of target population were involved in the 57 models, while heart failure (14/57, 25%) and a general population with or without cardiovascular risk factor(s) (12/57, 21%) received the most attention from the guidelines. The assessment of the methodological quality of 57 primary studies on the development of the guideline-recommended models revealed that only 40% of the modeling studies had a low risk of bias (ROB). The causes of high ROB were mainly in the analysis and participant domains.</p><p><strong>Conclusions: </strong>Global cardiovascular CPGs presented an unduly positive appraisal of the existing prediction models in terms of ROB, leading to stronger recommendations than were warranted. Future cardiovascular practice may benefit from well-established clinical prediction models with better methodological quality and extensive external validation.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1449058"},"PeriodicalIF":2.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coronary obstruction analysis in transcatheter aortic valve implantation through patient-specific computational modelling. 通过患者特异性计算建模分析经导管主动脉瓣植入术中的冠状动脉阻塞情况。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1432235
Jiaqi Fan, Jun Chen, Lihan Wang, Po Hu, Jubo Jiang, Xinping Lin, Giorgia Rocatello, Matthieu De Beule, Yi Tie, Yifei Wang, Sihang Cheng, Xianbao Liu, Jian'an Wang

Background: Coronary obstruction (CO) is a rare but devasting complication during transcatheter aortic valve replacement (TAVR).

Objectives: We aim to demonstrate that the predicted distance between the coronary ostia and the closest structure derived with patient-specific computer simulation is associated with CO risk during TAVR.

Methods: We retrospectively analysed 14 aortic stenosis patients who underwent TAVR through finite element simulation. The frame deformation predicted with patient-specific computer simulation was qualitatively and quantitatively compared to the post-operative device deformation. The minimum distance between each coronary ostium and the closest structure was calculated and compared in patients who developed CO, at high risk of CO, and at no risk of CO.

Results: Four patients experienced CO during TAVR, 5 patients were at high risk of CO, and the remaining 5 patients had no risk of CO. A high coefficient of determination was obtained for all measurements extracted from the simulated device and the post-operative device (≥0.95). Simulations predicted shorter distance between the coronary ostium and the closest structure in patients who experienced CO, compared to patients at high risk of CO or who did not experience this complication (right coronary: 5.9 vs. 6.8 vs. 8.8 mm, left coronary: 3.0 vs. 3.3 vs. 6.5 mm respectively).

Conclusions: The distance between the coronary ostium and the closest structure was lower in patients who experienced CO during TAVR through patient-specific computational simulation. This technology enables coronary obstruction analysis before TAVR in the future.

背景:冠状动脉阻塞(CO)是经导管主动脉瓣置换术(TAVR)中一种罕见但严重的并发症:我们的目的是证明,通过患者特异性计算机模拟得出的冠状动脉口与最近结构之间的预测距离与TAVR期间的冠状动脉阻塞风险有关:方法:我们通过有限元模拟对 14 名接受 TAVR 的主动脉瓣狭窄患者进行了回顾性分析。我们将患者特定计算机模拟预测的支架变形与术后设备变形进行了定性和定量比较。计算了每个冠状动脉骨膜与最近结构之间的最小距离,并对出现 CO、CO 高风险和无 CO 风险的患者进行了比较:结果:4 名患者在 TAVR 期间发生了 CO,5 名患者有 CO 的高风险,其余 5 名患者没有 CO 的风险。从模拟设备和术后设备中提取的所有测量值的决定系数都很高(≥0.95)。与 CO 高风险患者或未发生 CO 并发症的患者相比,模拟预测发生 CO 的患者冠状动脉骨膜与最近结构之间的距离较短(右冠状动脉:5.9 mm vs. 6.8 mm vs. 8.8 mm,左冠状动脉:3.0 mm vs. 3.3 mm vs. 6.5 mm):结论:通过患者特异性计算模拟,在TAVR过程中出现CO的患者冠状动脉骨膜与最近结构之间的距离较小。该技术可在未来的 TAVR 术前进行冠状动脉阻塞分析。
{"title":"Coronary obstruction analysis in transcatheter aortic valve implantation through patient-specific computational modelling.","authors":"Jiaqi Fan, Jun Chen, Lihan Wang, Po Hu, Jubo Jiang, Xinping Lin, Giorgia Rocatello, Matthieu De Beule, Yi Tie, Yifei Wang, Sihang Cheng, Xianbao Liu, Jian'an Wang","doi":"10.3389/fcvm.2024.1432235","DOIUrl":"10.3389/fcvm.2024.1432235","url":null,"abstract":"<p><strong>Background: </strong>Coronary obstruction (CO) is a rare but devasting complication during transcatheter aortic valve replacement (TAVR).</p><p><strong>Objectives: </strong>We aim to demonstrate that the predicted distance between the coronary ostia and the closest structure derived with patient-specific computer simulation is associated with CO risk during TAVR.</p><p><strong>Methods: </strong>We retrospectively analysed 14 aortic stenosis patients who underwent TAVR through finite element simulation. The frame deformation predicted with patient-specific computer simulation was qualitatively and quantitatively compared to the post-operative device deformation. The minimum distance between each coronary ostium and the closest structure was calculated and compared in patients who developed CO, at high risk of CO, and at no risk of CO.</p><p><strong>Results: </strong>Four patients experienced CO during TAVR, 5 patients were at high risk of CO, and the remaining 5 patients had no risk of CO. A high coefficient of determination was obtained for all measurements extracted from the simulated device and the post-operative device (≥0.95). Simulations predicted shorter distance between the coronary ostium and the closest structure in patients who experienced CO, compared to patients at high risk of CO or who did not experience this complication (right coronary: 5.9 vs. 6.8 vs. 8.8 mm, left coronary: 3.0 vs. 3.3 vs. 6.5 mm respectively).</p><p><strong>Conclusions: </strong>The distance between the coronary ostium and the closest structure was lower in patients who experienced CO during TAVR through patient-specific computational simulation. This technology enables coronary obstruction analysis before TAVR in the future.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1432235"},"PeriodicalIF":2.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between fecal incontinence and cardiovascular disease in adult Americans: evidence from NHANES 2005-2010. 美国成年人大便失禁与心血管疾病之间的关系:2005-2010 年 NHANES 提供的证据。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1447913
Chenkai Xu, Yongfu Song, Yuejiao Lan, Yongji Wang, Na Wang, Xiaodan Lu

Objectives: There is limited amount of research on the association between fecal incontinence (FI) and cardiovascular disease (CVD). This study aims to evaluate whether there is a relationship between FI and CVD among adults in the United States.

Methods: This study employed a cross-sectional design, encompassing 11,237 adults aged 20 years and older, drawn from the National Health and Nutrition Survey conducted from 2005 to 2010. FI was defined as the involuntary monthly leakage of solid, liquid, or mucus stool. The presence of CVD was evaluated through a questionnaire. Adjusted odds ratios (OR) were computed utilizing a multivariate logistic regression model. Subgroup analyses were conducted to ascertain the stability of the results.

Results: Following adjustments for population characteristics, lifestyle habits, laboratory tests, and comorbidities, a significant association was observed between FI and elevated CVD risk (OR: 1.47, 95% CI: 1.21-1.79, P < 0.001). Subgroup analysis uncovered a strong correlation between FI and CVD among participants aged 45-65 years (OR: 1.78, 95%CI: 1.31-2.43). In the participants to aged 66 and above, this correlation persisted (OR: 1.31, 95% CI: 1.01-1.70).

Conclusions: This study reveals a significant positive correlation between FI and CVD. Middle-aged and older adults are considered high-risk population for developing CVD, thus emphasizing the importance of screening and timely intervention.

研究目的有关大便失禁(FI)与心血管疾病(CVD)之间关系的研究数量有限。本研究旨在评估美国成年人大便失禁与心血管疾病之间是否存在关系:本研究采用横断面设计,从 2005 年至 2010 年进行的全国健康与营养调查中抽取了 11,237 名 20 岁及以上的成年人。FI的定义是每月不自主地排出固体、液体或粘液粪便。是否患有心血管疾病通过问卷进行评估。利用多变量逻辑回归模型计算调整后的几率比(OR)。为确定结果的稳定性,还进行了分组分析:在对人群特征、生活习惯、实验室检查和合并症进行调整后,观察到 FI 与心血管疾病风险升高之间存在显著关联(OR:1.47,95% CI:1.21-1.79,P 结论:该研究揭示了 FI 与心血管疾病风险升高之间的显著正相关:本研究揭示了 FI 与心血管疾病之间的显著正相关性。中老年人被认为是心血管疾病的高危人群,因此强调了筛查和及时干预的重要性。
{"title":"Association between fecal incontinence and cardiovascular disease in adult Americans: evidence from NHANES 2005-2010.","authors":"Chenkai Xu, Yongfu Song, Yuejiao Lan, Yongji Wang, Na Wang, Xiaodan Lu","doi":"10.3389/fcvm.2024.1447913","DOIUrl":"10.3389/fcvm.2024.1447913","url":null,"abstract":"<p><strong>Objectives: </strong>There is limited amount of research on the association between fecal incontinence (FI) and cardiovascular disease (CVD). This study aims to evaluate whether there is a relationship between FI and CVD among adults in the United States.</p><p><strong>Methods: </strong>This study employed a cross-sectional design, encompassing 11,237 adults aged 20 years and older, drawn from the National Health and Nutrition Survey conducted from 2005 to 2010. FI was defined as the involuntary monthly leakage of solid, liquid, or mucus stool. The presence of CVD was evaluated through a questionnaire. Adjusted odds ratios (OR) were computed utilizing a multivariate logistic regression model. Subgroup analyses were conducted to ascertain the stability of the results.</p><p><strong>Results: </strong>Following adjustments for population characteristics, lifestyle habits, laboratory tests, and comorbidities, a significant association was observed between FI and elevated CVD risk (OR: 1.47, 95% CI: 1.21-1.79, <i>P</i> < 0.001). Subgroup analysis uncovered a strong correlation between FI and CVD among participants aged 45-65 years (OR: 1.78, 95%CI: 1.31-2.43). In the participants to aged 66 and above, this correlation persisted (OR: 1.31, 95% CI: 1.01-1.70).</p><p><strong>Conclusions: </strong>This study reveals a significant positive correlation between FI and CVD. Middle-aged and older adults are considered high-risk population for developing CVD, thus emphasizing the importance of screening and timely intervention.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1447913"},"PeriodicalIF":2.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identifying HIF1A and HGF as two hub genes in aortic dissection and function analysis by integrating RNA sequencing and single-cell RNA sequencing data. 通过整合 RNA 测序和单细胞 RNA 测序数据,确定 HIF1A 和 HGF 是主动脉夹层中的两个枢纽基因并进行功能分析。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1475991
Hai-Bing Li, Chang Liu, Xiang-Di Mao, Shu-Zheng Yuan, Li Li, Xin Cong
<p><strong>Objective: </strong>Aortic dissection (AD) is a severe aortic disease with high mortality, and its pathogenesis remains elusive. To explore the regulatory mechanisms of AD, we integrated public RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) datasets to screen the hub genes of AD and further analyzed their functions, which may provide references to the diagnosis and treatment of AD.</p><p><strong>Methods: </strong>Four AD-related datasets were obtained from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis and differential expression analysis were applied to identify overlapping genes in dataset GSE153434. Protein-protein interaction (PPI) network was constructed based on overlapping genes. Five methods (closeness, degree, EPC, MCC, and MNN) were used to pick hub genes. The receiver operating characteristic curve was used to evaluate the diagnostic efficiency of the hub genes in extra datasets GSE98770 and GSE52093. scRNA-seq dataset GSE213740 was used to explore the expression and function of the hub genes at the single-cell level. Quantitative real-time polymerase chain reaction was used to verify the expression of hub genes in beta-aminopropionitrile (BAPN)-induced mouse thoracic aortic aneurysm and dissection (TAAD) model.</p><p><strong>Results: </strong>A total of 71 overlapping genes were screened by intersecting the significant genes in the pink module and the differentially expressed genes. A PPI network with 45 nodes and 74 edges was generated, and five top hub genes (<i>HIF1A</i>, <i>HGF</i>, <i>HMOX1</i>, <i>ITGA5</i>, and <i>ITGB3</i>) were identified. All the hub genes had area under the curve values above 0.55. scRNA-seq data analysis showed that <i>HIF1A</i> was significantly upregulated in macrophages and <i>HGF</i> was significantly upregulated in vascular smooth muscle cells (SMCs) of the ascending aortas in AD patients. HIF1A may transcriptionally regulate multiple downstream target genes involving inflammation (<i>TLR2</i>, <i>ALOX5AP</i>, and <i>MIF</i>), glycolysis (<i>ENO1</i>, <i>LDHA</i>, and <i>GAPDH</i>), tissue remodeling (<i>PLAU</i>), and angiogenesis (<i>SERPIN</i> and <i>VEGFA</i>). HGF may participate in the signaling among SMCs, fibroblasts, and endothelial cells through binding to different receptors (MET, EGFR, IGF1R, and KDR). The mRNA expression of <i>Hif1a</i>, <i>Hgf</i>, and their target genes, including <i>Alox5ap</i>, <i>Serpine1</i>, <i>Tlr2</i>, <i>Plau</i>, <i>Egfr</i>, and <i>Igf1r</i>, was significantly upregulated in aortic tissues of BAPN-treated mice.</p><p><strong>Conclusion: </strong>By integrating RNA-seq and scRNA-seq data, we identified <i>HIF1A</i> and <i>HGF</i> as two hub genes with good diagnostic efficiency for AD. HIF1A in macrophages may promote AD formation by promoting inflammation, glycolysis, tissue remodeling, and angiogenesis, and HGF may mediate signaling among SMCs, fibroblasts, and endothelial cells in the
目的:主动脉夹层(AD)是一种严重的主动脉疾病,死亡率很高,其发病机制至今仍不清楚。为了探索AD的调控机制,我们整合了公共RNA测序(RNA-seq)和单细胞RNA测序(scRNA-seq)数据集,筛选出了AD的枢纽基因,并进一步分析了它们的功能,这可能为AD的诊断和治疗提供参考:方法:从基因表达总库(GEO)数据库中获取四个与AD相关的数据集。方法:从基因表达总库(GEO)数据库中获取四个与AD相关的数据集,应用加权基因共表达网络分析和差异表达分析找出数据集GSE153434中的重叠基因。根据重叠基因构建了蛋白质-蛋白质相互作用(PPI)网络。使用五种方法(接近度、度、EPC、MCC 和 MNN)挑选中心基因。接收者操作特征曲线用于评估额外数据集 GSE98770 和 GSE52093 中枢纽基因的诊断效率。scRNA-seq 数据集 GSE213740 用于在单细胞水平探索枢纽基因的表达和功能。利用定量实时聚合酶链反应验证了β-氨基丙腈(BAPN)诱导的小鼠胸主动脉瘤和夹层(TAAD)模型中枢纽基因的表达:通过将粉色模块中的重要基因与差异表达基因交叉,共筛选出71个重叠基因。生成了一个有 45 个节点和 74 条边的 PPI 网络,并确定了五个顶级中心基因(HIF1A、HGF、HMOX1、ITGA5 和 ITGB3)。scRNA-seq数据分析显示,HIF1A在AD患者升主动脉的巨噬细胞中显著上调,HGF在血管平滑肌细胞(SMC)中显著上调。HIF1A 可转录调控多个下游靶基因,涉及炎症(TLR2、ALOX5AP 和 MIF)、糖酵解(ENO1、LDHA 和 GAPDH)、组织重塑(PLAU)和血管生成(SERPIN 和 VEGFA)。HGF 可通过与不同的受体(MET、表皮生长因子受体、IGF1R 和 KDR)结合,参与 SMC、成纤维细胞和内皮细胞之间的信号转导。Hif1a、Hgf及其靶基因(包括Alox5ap、Serpine1、Tlr2、Plau、Egfr和Igf1r)的mRNA表达在BAPN处理的小鼠主动脉组织中显著上调:通过整合RNA-seq和scRNA-seq数据,我们发现HIF1A和HGF是两个对AD具有良好诊断效果的枢纽基因。巨噬细胞中的 HIF1A 可通过促进炎症、糖酵解、组织重塑和血管生成来促进 AD 的形成,而 HGF 可在 AD 的发生过程中介导 SMC、成纤维细胞和内皮细胞之间的信号转导。
{"title":"Identifying <i>HIF1A</i> and <i>HGF</i> as two hub genes in aortic dissection and function analysis by integrating RNA sequencing and single-cell RNA sequencing data.","authors":"Hai-Bing Li, Chang Liu, Xiang-Di Mao, Shu-Zheng Yuan, Li Li, Xin Cong","doi":"10.3389/fcvm.2024.1475991","DOIUrl":"10.3389/fcvm.2024.1475991","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Aortic dissection (AD) is a severe aortic disease with high mortality, and its pathogenesis remains elusive. To explore the regulatory mechanisms of AD, we integrated public RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) datasets to screen the hub genes of AD and further analyzed their functions, which may provide references to the diagnosis and treatment of AD.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Four AD-related datasets were obtained from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis and differential expression analysis were applied to identify overlapping genes in dataset GSE153434. Protein-protein interaction (PPI) network was constructed based on overlapping genes. Five methods (closeness, degree, EPC, MCC, and MNN) were used to pick hub genes. The receiver operating characteristic curve was used to evaluate the diagnostic efficiency of the hub genes in extra datasets GSE98770 and GSE52093. scRNA-seq dataset GSE213740 was used to explore the expression and function of the hub genes at the single-cell level. Quantitative real-time polymerase chain reaction was used to verify the expression of hub genes in beta-aminopropionitrile (BAPN)-induced mouse thoracic aortic aneurysm and dissection (TAAD) model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 71 overlapping genes were screened by intersecting the significant genes in the pink module and the differentially expressed genes. A PPI network with 45 nodes and 74 edges was generated, and five top hub genes (&lt;i&gt;HIF1A&lt;/i&gt;, &lt;i&gt;HGF&lt;/i&gt;, &lt;i&gt;HMOX1&lt;/i&gt;, &lt;i&gt;ITGA5&lt;/i&gt;, and &lt;i&gt;ITGB3&lt;/i&gt;) were identified. All the hub genes had area under the curve values above 0.55. scRNA-seq data analysis showed that &lt;i&gt;HIF1A&lt;/i&gt; was significantly upregulated in macrophages and &lt;i&gt;HGF&lt;/i&gt; was significantly upregulated in vascular smooth muscle cells (SMCs) of the ascending aortas in AD patients. HIF1A may transcriptionally regulate multiple downstream target genes involving inflammation (&lt;i&gt;TLR2&lt;/i&gt;, &lt;i&gt;ALOX5AP&lt;/i&gt;, and &lt;i&gt;MIF&lt;/i&gt;), glycolysis (&lt;i&gt;ENO1&lt;/i&gt;, &lt;i&gt;LDHA&lt;/i&gt;, and &lt;i&gt;GAPDH&lt;/i&gt;), tissue remodeling (&lt;i&gt;PLAU&lt;/i&gt;), and angiogenesis (&lt;i&gt;SERPIN&lt;/i&gt; and &lt;i&gt;VEGFA&lt;/i&gt;). HGF may participate in the signaling among SMCs, fibroblasts, and endothelial cells through binding to different receptors (MET, EGFR, IGF1R, and KDR). The mRNA expression of &lt;i&gt;Hif1a&lt;/i&gt;, &lt;i&gt;Hgf&lt;/i&gt;, and their target genes, including &lt;i&gt;Alox5ap&lt;/i&gt;, &lt;i&gt;Serpine1&lt;/i&gt;, &lt;i&gt;Tlr2&lt;/i&gt;, &lt;i&gt;Plau&lt;/i&gt;, &lt;i&gt;Egfr&lt;/i&gt;, and &lt;i&gt;Igf1r&lt;/i&gt;, was significantly upregulated in aortic tissues of BAPN-treated mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;By integrating RNA-seq and scRNA-seq data, we identified &lt;i&gt;HIF1A&lt;/i&gt; and &lt;i&gt;HGF&lt;/i&gt; as two hub genes with good diagnostic efficiency for AD. HIF1A in macrophages may promote AD formation by promoting inflammation, glycolysis, tissue remodeling, and angiogenesis, and HGF may mediate signaling among SMCs, fibroblasts, and endothelial cells in the ","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1475991"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The incremental value of interatrial septum motion in predicting thrombus or spontaneous echo contrast in patients with non-valvular atrial fibrillation: an observational study on transesophageal echocardiography. 房间隔运动在预测非瓣膜性心房颤动患者血栓或自发回声对比中的增量价值:一项经食道超声心动图观察性研究。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1366180
Decai Zeng, Shuai Chang, Xiaofeng Zhang, Yanfeng Zhong, Cai Yongzhi, Tongtong Huang, Ji Wu

Background: The thickness and motion of the Interatrial Septum (IAS) possibly serves as indicators of both structural and functional remodeling of left atrium. This study aims to use transesophageal echocardiography (TEE) to assess IAS motion in non-valvular atrial fibrillation (NVAF) and investigate its correlation with the risk of spontaneous echo contrast (SEC) and thrombus (TH).

Methods: We conducted a cross-sectional study on 318 patients with NVAF who underwent transthoracic echocardiography and TEE. IAS motion was defined as the maximum displacement of IAS observed throughout the cardiac cycles using M-mode TEE.

Results: The prevalence of SEC/TH was 39.9% (127/318) in the overall group. In paroxysmal atrial fibrillation (PAF), the prevalence rate of SEC/TH was 25.3% (47/186), compared to 60.6% (80/132) in persistent atrial fibrillation (PeAF). Multivariable analyses showed that LA volume index (LAVI), mean E/e', S/D ratio, IAS motion and CHA2DS2-VASc scores were significantly associated with SEC/TH. Patients with lower IAS motion showed a higher prevalence of SEC/TH compared to those with higher IAS motion (71.3% vs. 11.6%; P < 0.001). The IAS motion under sinus rhythm could better predict risk of SEC/TH, while the predictive efficacy under AF rhythm was slightly attenuated but still had a high AUC value (0.779). A significant positive correlation was observed between the IAS motion and the LAA filling velocity (PAF r = 0.47; P<0.001 and PeAF r = 0.38; P < 0.001, respectively), LAA emptying velocity (PAF r = 0.55; P < 0.001 and PeAF r = 0.47; P < 0.001, respectively) and LAVI (PAF r = 0.59; P < 0.001 and PeAF r = 0.44; P < 0.001, respectively). The integration of the IAS motion to the CHA2DS2-VASc, LAVI and mean E/e' provided important incremental predictive value of SEC/TH (AUC = 0.859 vs. 0.826, P = 0.02).

Conclusion: IAS motion measured by TEE correlates well with LAA flow velocity and LA size and is independently associated with SEC/TH in NVAF. Additionally, lower IAS motion is associated with a higher prevalence of SEC/TH. Furthermore, the integration of IAS motion to CHA2DS2-VASc, LAVI and mean E/e' can provide additional value for the prediction of SEC/TH.

背景:房间隔(IAS)的厚度和运动可能是左心房结构和功能重塑的指标。本研究旨在使用经食道超声心动图(TEE)评估非瓣膜性心房颤动(NVAF)患者的 IAS 运动,并研究其与自发回声对比(SEC)和血栓(TH)风险的相关性:我们对 318 名接受经胸超声心动图和 TEE 检查的 NVAF 患者进行了横断面研究。IAS运动定义为使用M型TEE观察到的整个心动周期中IAS的最大位移:结果:SEC/TH的发病率为39.9%(127/318)。在阵发性心房颤动(PAF)中,SEC/TH的患病率为25.3%(47/186),而在持续性心房颤动(PeAF)中,SEC/TH的患病率为60.6%(80/132)。多变量分析显示,LA容积指数(LAVI)、平均E/e'、S/D比值、IAS运动和CHA2DS2-VASc评分与SEC/TH显著相关。与 IAS 运动较高的患者相比,IAS 运动较低的患者 SEC/TH 患病率更高(71.3% vs. 11.6%;P r = 0.47;Pr = 0.38;P r = 0.55;P r = 0.47;P r = 0.59;P r = 0.44;P P = 0.02):结论:通过 TEE 测量的 IAS 运动与 LAA 流速和 LA 大小密切相关,并且与 NVAF 的 SEC/TH 独立相关。此外,较低的 IAS 运动与较高的 SEC/TH 发生率相关。此外,将 IAS 运动与 CHA2DS2-VASc、LAVI 和平均 E/e' 整合可为 SEC/TH 的预测提供额外价值。
{"title":"The incremental value of interatrial septum motion in predicting thrombus or spontaneous echo contrast in patients with non-valvular atrial fibrillation: an observational study on transesophageal echocardiography.","authors":"Decai Zeng, Shuai Chang, Xiaofeng Zhang, Yanfeng Zhong, Cai Yongzhi, Tongtong Huang, Ji Wu","doi":"10.3389/fcvm.2024.1366180","DOIUrl":"10.3389/fcvm.2024.1366180","url":null,"abstract":"<p><strong>Background: </strong>The thickness and motion of the Interatrial Septum (IAS) possibly serves as indicators of both structural and functional remodeling of left atrium. This study aims to use transesophageal echocardiography (TEE) to assess IAS motion in non-valvular atrial fibrillation (NVAF) and investigate its correlation with the risk of spontaneous echo contrast (SEC) and thrombus (TH).</p><p><strong>Methods: </strong>We conducted a cross-sectional study on 318 patients with NVAF who underwent transthoracic echocardiography and TEE. IAS motion was defined as the maximum displacement of IAS observed throughout the cardiac cycles using M-mode TEE.</p><p><strong>Results: </strong>The prevalence of SEC/TH was 39.9% (127/318) in the overall group. In paroxysmal atrial fibrillation (PAF), the prevalence rate of SEC/TH was 25.3% (47/186), compared to 60.6% (80/132) in persistent atrial fibrillation (PeAF). Multivariable analyses showed that LA volume index (LAVI), mean E/e', S/D ratio, IAS motion and CHA2DS2-VASc scores were significantly associated with SEC/TH. Patients with lower IAS motion showed a higher prevalence of SEC/TH compared to those with higher IAS motion (71.3% vs. 11.6%; <i>P</i> < 0.001). The IAS motion under sinus rhythm could better predict risk of SEC/TH, while the predictive efficacy under AF rhythm was slightly attenuated but still had a high AUC value (0.779). A significant positive correlation was observed between the IAS motion and the LAA filling velocity (PAF <i>r</i> = 0.47; <i>P</i><0.001 and PeAF <i>r</i> = 0.38; <i>P</i> < 0.001, respectively), LAA emptying velocity (PAF <i>r</i> = 0.55; <i>P</i> < 0.001 and PeAF <i>r</i> = 0.47; <i>P</i> < 0.001, respectively) and LAVI (PAF <i>r</i> = 0.59; <i>P</i> < 0.001 and PeAF <i>r</i> = 0.44; <i>P</i> < 0.001, respectively). The integration of the IAS motion to the CHA2DS2-VASc, LAVI and mean E/e' provided important incremental predictive value of SEC/TH (AUC = 0.859 vs. 0.826, <i>P</i> = 0.02).</p><p><strong>Conclusion: </strong>IAS motion measured by TEE correlates well with LAA flow velocity and LA size and is independently associated with SEC/TH in NVAF. Additionally, lower IAS motion is associated with a higher prevalence of SEC/TH. Furthermore, the integration of IAS motion to CHA2DS2-VASc, LAVI and mean E/e' can provide additional value for the prediction of SEC/TH.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1366180"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of gut microbiome in mediating the effect of inflammatory bowel disease on hypertension: a two-step, two-sample Mendelian randomization study. 肠道微生物组在介导炎症性肠病对高血压的影响中的作用:一项两步双样本孟德尔随机研究。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1396973
Wei Bao, Yan Zhang, Xiao-Jia Huang, Ning Gu

Objective: Investigating the causal connection that exists between inflammatory bowel disease (IBD) and hypertension (HT). To gain a deeper insight into the correlation among IBD, gut microbiota, and HT, we conducted a two-step, two-sample Mendelian randomization study.

Methods: An investigation of genome-wide association study (GWAS) summary-level data was utilized to conduct a two-sample Mendelian randomization (MR) analysis of genetically predicted inflammatory bowel disease: (12,882cases, 21,770controls) on Systolic/Diastolic blood pressure (N = 2,564). Subsequently, two-step MR analyses revealed that the relationship between IBD and SBP was partly mediated by Faecalicatena glycyrrhizinilyticum. The robustness of the findings was confirmed through several sensitivity assessments.

Results: This MR study showed that increase in genetically predicted IBD was associated with higher risk of genetically predicted SBP (OR: 1.08, 95% CI: 1.01-1.16, P < 0.05) and DBP (OR: 1.09, 95% CI: 1.02-1.17, P < 0.05), respectively. Inverse variance weighted (IVW) MR analysis also showed that increase in genetically predicted IBD was associated with higher abundance Faecalicatena glycyrrhizinilyticum (OR: 1.03, 95% CI: 1.01-1.04, P < 0.05), which subsequently associated with increased SBP risk (OR: 1.42, 95% CI: 1.06-1.9, P < 0.05). Faecalicatena glycyrrhizinilyticum abundance in stool was responsible for mediating 11% of the genetically predicted IBD on SBP.

Conclusion: The research proposed a causal link between Inflammatory Bowel Disease (IBD) and Hypertension (HT), with a little percentage of the impact being influenced by Faecalicatena glycyrrhizinilyticum in stool. Mitigating gut microbiome may decrease the heightened risk of hypertension in people with inflammatory bowel disease.

目的:调查炎症性肠病(IBD)与高血压(HT)之间的因果关系:研究炎症性肠病(IBD)与高血压(HT)之间的因果关系。为了更深入地了解 IBD、肠道微生物群和高血压之间的相关性,我们进行了一项两步双样本孟德尔随机研究:利用对全基因组关联研究(GWAS)汇总级数据的调查,对收缩压/舒张压(N = 2,564)上的遗传预测炎症性肠病(12,882 例,21,770 个对照)进行了两步孟德尔随机化(MR)分析。随后,两步磁共振分析显示,IBD 和 SBP 之间的关系部分是由甘草酸钙酵母介导的。通过几项敏感性评估,证实了研究结果的稳健性:这项磁共振研究表明,遗传预测的 IBD 增加与遗传预测的 SBP 风险升高有关(OR:1.08,95% CI:1.01-1.16,P P):该研究提出了炎症性肠病(IBD)与高血压(HT)之间的因果关系,而粪便中甘草酸钾菌的影响只占很小的比例。减轻肠道微生物群可能会降低炎症性肠病患者罹患高血压的风险。
{"title":"The role of gut microbiome in mediating the effect of inflammatory bowel disease on hypertension: a two-step, two-sample Mendelian randomization study.","authors":"Wei Bao, Yan Zhang, Xiao-Jia Huang, Ning Gu","doi":"10.3389/fcvm.2024.1396973","DOIUrl":"10.3389/fcvm.2024.1396973","url":null,"abstract":"<p><strong>Objective: </strong>Investigating the causal connection that exists between inflammatory bowel disease (IBD) and hypertension (HT). To gain a deeper insight into the correlation among IBD, gut microbiota, and HT, we conducted a two-step, two-sample Mendelian randomization study.</p><p><strong>Methods: </strong>An investigation of genome-wide association study (GWAS) summary-level data was utilized to conduct a two-sample Mendelian randomization (MR) analysis of genetically predicted inflammatory bowel disease: (12,882cases, 21,770controls) on Systolic/Diastolic blood pressure (<i>N</i> = 2,564). Subsequently, two-step MR analyses revealed that the relationship between IBD and SBP was partly mediated by <i>Faecalicatena glycyrrhizinilyticum</i>. The robustness of the findings was confirmed through several sensitivity assessments.</p><p><strong>Results: </strong>This MR study showed that increase in genetically predicted IBD was associated with higher risk of genetically predicted SBP (OR: 1.08, 95% CI: 1.01-1.16, <i>P</i> < 0.05) and DBP (OR: 1.09, 95% CI: 1.02-1.17, <i>P</i> < 0.05), respectively. Inverse variance weighted (IVW) MR analysis also showed that increase in genetically predicted IBD was associated with higher abundance <i>Faecalicatena glycyrrhizinilyticum</i> (OR: 1.03, 95% CI: 1.01-1.04, <i>P</i> < 0.05), which subsequently associated with increased SBP risk (OR: 1.42, 95% CI: 1.06-1.9, <i>P</i> < 0.05). <i>Faecalicatena glycyrrhizinilyticum</i> abundance in stool was responsible for mediating 11% of the genetically predicted IBD on SBP.</p><p><strong>Conclusion: </strong>The research proposed a causal link between Inflammatory Bowel Disease (IBD) and Hypertension (HT), with a little percentage of the impact being influenced by <i>Faecalicatena glycyrrhizinilyticum</i> in stool. Mitigating gut microbiome may decrease the heightened risk of hypertension in people with inflammatory bowel disease.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1396973"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: Sedentary behaviour and cardiometabolic health. 社论:久坐行为与心脏代谢健康。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1498410
Liane Beretta De Azevedo, Gabriel Grizzo Cucato, Bente Morseth
{"title":"Editorial: Sedentary behaviour and cardiometabolic health.","authors":"Liane Beretta De Azevedo, Gabriel Grizzo Cucato, Bente Morseth","doi":"10.3389/fcvm.2024.1498410","DOIUrl":"10.3389/fcvm.2024.1498410","url":null,"abstract":"","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1498410"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing the relative importance of vitamin D deficiency in cardiovascular health. 评估维生素 D 缺乏对心血管健康的相对重要性。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1435738
Maira Rubab, John D Kelleher

Previous research has suggested a potential link between vitamin D (VD) deficiency and adverse cardiovascular health outcomes, although the findings have been inconsistent. This study investigates the association between VD deficiency and cardiovascular disease (CVD) within the context of established CVD risk factors. We utilized a Random Forest model to predict both CVD and VD deficiency risks, using a dataset of 1,078 observations from a rural Chinese population. Feature importance was evaluated using SHapley Additive exPlanations (SHAP) to discern the impact of various risk factors on the model's output. The results showed that the model for CVD prediction achieved a high accuracy of 87%, demonstrating robust performance across precision, recall, and F1 score metrics. Conversely, the VD deficiency prediction model exhibited suboptimal performance, with an accuracy of 52% and lower precision, recall, and F1 scores. Feature importance analysis indicated that traditional risk factors such as systolic blood pressure, diastolic blood pressure, age, body mass index, and waist-to-hip ratio significantly influenced CVD risk, collectively contributing to 70% of the model's predictive power. Although VD deficiency was associated with an increased risk of CVD, its importance in predicting CVD risk was notably low. Similarly, for VD deficiency prediction, CVD risk factors such as systolic blood pressure, glucose levels, diastolic blood pressure, and body mass index emerged as influential features. However, the overall predictive performance of the VD deficiency prediction model was weak (52%), indicating the absence of VD deficiency-related risk factors. Ablation experiments confirmed the relatively lower importance of VD deficiency in predicting CVD risk. Furthermore, the SHAP partial dependence plot revealed a nonlinear relationship between VD levels and CVD risk. In conclusion, while VD deficiency appears directly or indirectly associated with increased CVD risk, its relative importance within predictive models is considerably lower when compared to other risk factors. These findings suggest that VD deficiency may not warrant primary focus in CVD risk assessment and prevention strategies, however, further research is needed to explore the causal relationship between VD deficiency and CVD risk.

以往的研究表明,维生素 D(VD)缺乏与不良心血管健康后果之间存在潜在联系,但研究结果并不一致。本研究结合已确定的心血管疾病(CVD)风险因素,调查了维生素 D 缺乏与心血管疾病(CVD)之间的关联。我们使用随机森林模型预测心血管疾病和 VD 缺乏的风险,数据集包含来自中国农村人口的 1,078 个观测值。使用SHapley Additive exPlanations(SHAP)评估了特征的重要性,以辨别各种风险因素对模型输出结果的影响。结果表明,心血管疾病预测模型的准确率高达 87%,在精确度、召回率和 F1 分数指标上都表现出稳健的性能。相反,VD 缺乏症预测模型表现不佳,准确率为 52%,精确度、召回率和 F1 分数都较低。特征重要性分析表明,收缩压、舒张压、年龄、体重指数和腰臀比等传统风险因素对心血管疾病风险有显著影响,共占模型预测能力的 70%。尽管VD缺乏与心血管疾病风险的增加有关,但其在预测心血管疾病风险方面的重要性明显较低。同样,对于 VD 缺乏症的预测,收缩压、血糖水平、舒张压和体重指数等心血管疾病风险因素也是有影响的特征。然而,VD 缺乏症预测模型的整体预测性能较弱(52%),表明缺乏 VD 缺乏症相关的风险因素。消融实验证实,VD 缺乏在预测心血管疾病风险方面的重要性相对较低。此外,SHAP 部分依存图显示 VD 水平与心血管疾病风险之间存在非线性关系。总之,尽管VD缺乏似乎与心血管疾病风险的增加直接或间接相关,但与其他风险因素相比,其在预测模型中的相对重要性要低得多。这些研究结果表明,在心血管疾病风险评估和预防策略中,VD 缺乏可能不值得重点关注,但是,还需要进一步研究来探讨 VD 缺乏与心血管疾病风险之间的因果关系。
{"title":"Assessing the relative importance of vitamin D deficiency in cardiovascular health.","authors":"Maira Rubab, John D Kelleher","doi":"10.3389/fcvm.2024.1435738","DOIUrl":"10.3389/fcvm.2024.1435738","url":null,"abstract":"<p><p>Previous research has suggested a potential link between vitamin D (VD) deficiency and adverse cardiovascular health outcomes, although the findings have been inconsistent. This study investigates the association between VD deficiency and cardiovascular disease (CVD) within the context of established CVD risk factors. We utilized a Random Forest model to predict both CVD and VD deficiency risks, using a dataset of 1,078 observations from a rural Chinese population. Feature importance was evaluated using SHapley Additive exPlanations (SHAP) to discern the impact of various risk factors on the model's output. The results showed that the model for CVD prediction achieved a high accuracy of 87%, demonstrating robust performance across precision, recall, and F1 score metrics. Conversely, the VD deficiency prediction model exhibited suboptimal performance, with an accuracy of 52% and lower precision, recall, and F1 scores. Feature importance analysis indicated that traditional risk factors such as systolic blood pressure, diastolic blood pressure, age, body mass index, and waist-to-hip ratio significantly influenced CVD risk, collectively contributing to 70% of the model's predictive power. Although VD deficiency was associated with an increased risk of CVD, its importance in predicting CVD risk was notably low. Similarly, for VD deficiency prediction, CVD risk factors such as systolic blood pressure, glucose levels, diastolic blood pressure, and body mass index emerged as influential features. However, the overall predictive performance of the VD deficiency prediction model was weak (52%), indicating the absence of VD deficiency-related risk factors. Ablation experiments confirmed the relatively lower importance of VD deficiency in predicting CVD risk. Furthermore, the SHAP partial dependence plot revealed a nonlinear relationship between VD levels and CVD risk. In conclusion, while VD deficiency appears directly or indirectly associated with increased CVD risk, its relative importance within predictive models is considerably lower when compared to other risk factors. These findings suggest that VD deficiency may not warrant primary focus in CVD risk assessment and prevention strategies, however, further research is needed to explore the causal relationship between VD deficiency and CVD risk.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1435738"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of angiogenesis and regenerative lung growth in Lepob/ob mice through adiponectin-VEGF/VEGFR2 signaling. 通过脂肪连接素-VEGF/VEGFR2 信号抑制 Lepob/ob 小鼠的血管生成和肺再生生长。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1491971
Tendai Hunyenyiwa, Priscilla Kyi, Mikaela Scheer, Mrudula Joshi, Mario Gasparri, Tadanori Mammoto, Akiko Mammoto

Introduction: Obesity is associated with impairment of wound healing and tissue regeneration. Angiogenesis, the formation of new blood capillaries, plays a key role in regenerative lung growth after unilateral pneumonectomy (PNX). We have reported that obesity inhibits angiogenesis. The effects of obesity on post-PNX lung vascular and alveolar regeneration remain unclear.

Methods: Unilateral PNX is performed on Lep o b / o b obese mice to examine vascular and alveolar regeneration.

Results: Regenerative lung growth and expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR2 induced after PNX are inhibited in Lep o b / o b obese mice. The levels of adiponectin that exhibits pro-angiogenic and vascular protective properties increase after unilateral PNX, while the effects are attenuated in Lep o b / o b obese mice. Post-PNX regenerative lung growth and increases in the levels of VEGF and VEGFR2 are inhibited in adiponectin knockout mice. Adiponectin stimulates angiogenic activities in human lung endothelial cells (ECs), which is inhibited by decreasing the levels of transcription factor Twist1. Adiponectin agonist, AdipoRon restores post-PNX lung growth and vascular and alveolar regeneration in Lep o b / o b obese mice.

Discussion: These findings suggest that obesity impairs lung vascular and alveolar regeneration and adiponectin is one of the key factors to improve lung regeneration in obese people.

简介肥胖与伤口愈合和组织再生障碍有关。血管生成(新毛细血管的形成)在单侧肺切除术(PNX)后的肺再生生长中起着关键作用。我们曾报道过肥胖会抑制血管生成。肥胖对单侧肺切除术后肺血管和肺泡再生的影响仍不清楚:方法:对 Lep o b / o b 肥胖小鼠进行单侧 PNX,以检查血管和肺泡再生情况:结果:Lep o b / o b 肥胖小鼠的肺再生生长和 PNX 后诱导的血管内皮生长因子(VEGF)及其受体 VEGFR2 的表达受到抑制。单侧 PNX 后,具有促血管生成和血管保护特性的脂肪连通素水平升高,而 Lep o b / o b 肥胖小鼠的这种效应减弱。在脂肪连通素基因敲除小鼠中,PNX 后肺再生生长以及血管内皮生长因子和血管内皮生长因子受体 2 水平的增加受到抑制。脂肪连接素能刺激人肺内皮细胞(ECs)的血管生成活性,而转录因子 Twist1 水平的降低能抑制血管生成活性。Adiponectin 激动剂 AdipoRon 可恢复 Lep o b / o b 肥胖小鼠 PNX 后的肺生长以及血管和肺泡再生:这些研究结果表明,肥胖会损害肺血管和肺泡再生,而脂肪连素是改善肥胖者肺再生的关键因素之一。
{"title":"Inhibition of angiogenesis and regenerative lung growth in <i>Lep<sup>ob/ob</sup></i> mice through adiponectin-VEGF/VEGFR2 signaling.","authors":"Tendai Hunyenyiwa, Priscilla Kyi, Mikaela Scheer, Mrudula Joshi, Mario Gasparri, Tadanori Mammoto, Akiko Mammoto","doi":"10.3389/fcvm.2024.1491971","DOIUrl":"10.3389/fcvm.2024.1491971","url":null,"abstract":"<p><strong>Introduction: </strong>Obesity is associated with impairment of wound healing and tissue regeneration. Angiogenesis, the formation of new blood capillaries, plays a key role in regenerative lung growth after unilateral pneumonectomy (PNX). We have reported that obesity inhibits angiogenesis. The effects of obesity on post-PNX lung vascular and alveolar regeneration remain unclear.</p><p><strong>Methods: </strong>Unilateral PNX is performed on <i>Lep</i> <sup><i>o</i> <i>b</i> <i>/</i> <i>o</i> <i>b</i></sup> obese mice to examine vascular and alveolar regeneration.</p><p><strong>Results: </strong>Regenerative lung growth and expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR2 induced after PNX are inhibited in <i>Lep</i> <sup><i>o</i> <i>b</i> <i>/</i> <i>o</i> <i>b</i></sup> obese mice. The levels of adiponectin that exhibits pro-angiogenic and vascular protective properties increase after unilateral PNX, while the effects are attenuated in <i>Lep</i> <sup><i>o</i> <i>b</i> <i>/</i> <i>o</i> <i>b</i></sup> obese mice. Post-PNX regenerative lung growth and increases in the levels of VEGF and VEGFR2 are inhibited in adiponectin knockout mice. Adiponectin stimulates angiogenic activities in human lung endothelial cells (ECs), which is inhibited by decreasing the levels of transcription factor Twist1. Adiponectin agonist, AdipoRon restores post-PNX lung growth and vascular and alveolar regeneration in <i>Lep</i> <sup><i>o</i> <i>b</i> <i>/</i> <i>o</i> <i>b</i></sup> obese mice.</p><p><strong>Discussion: </strong>These findings suggest that obesity impairs lung vascular and alveolar regeneration and adiponectin is one of the key factors to improve lung regeneration in obese people.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1491971"},"PeriodicalIF":2.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Case Report: Massive intrapericardial hematoma following acupuncture therapy. 病例报告:针灸治疗后心包内大量血肿。
IF 2.8 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI: 10.3389/fcvm.2024.1433945
Weiguang Chen, Xiaoling Gao, Hong Li

Cardiac tamponade is a critical cardiovascular condition where timely diagnosis and treatment are crucial. The formation of an intrapericardial hematoma following acupuncture therapy is clinically rare. This paper reports a case of an elderly female patient who experienced severe chest pain and syncope during acupuncture therapy, subsequently diagnosed with traumatic hemopericardium and acute cardiac tamponade, complicated by cardiogenic shock. Under ultrasound guidance, pericardial puncture and drainage were successfully performed. The patient's symptoms were alleviated, her vital signs stabilized, and follow-up outcomes were favorable. This case provides valuable reference for understanding the pathogenesis, diagnosis, and treatment of pericardial hemorrhage following acupuncture therapy, integrating both clinical practice and literature review.

心脏填塞是一种严重的心血管疾病,及时诊断和治疗至关重要。针灸治疗后形成心包内血肿在临床上并不多见。本文报告了一例老年女性患者在针灸治疗过程中出现剧烈胸痛和晕厥,随后被诊断为外伤性血心包和急性心脏填塞,并发心源性休克。在超声引导下,成功进行了心包穿刺和引流。患者症状缓解,生命体征稳定,随访结果良好。本病例结合临床实践和文献综述,为了解针灸治疗后心包出血的发病机制、诊断和治疗提供了有价值的参考。
{"title":"Case Report: Massive intrapericardial hematoma following acupuncture therapy.","authors":"Weiguang Chen, Xiaoling Gao, Hong Li","doi":"10.3389/fcvm.2024.1433945","DOIUrl":"https://doi.org/10.3389/fcvm.2024.1433945","url":null,"abstract":"<p><p>Cardiac tamponade is a critical cardiovascular condition where timely diagnosis and treatment are crucial. The formation of an intrapericardial hematoma following acupuncture therapy is clinically rare. This paper reports a case of an elderly female patient who experienced severe chest pain and syncope during acupuncture therapy, subsequently diagnosed with traumatic hemopericardium and acute cardiac tamponade, complicated by cardiogenic shock. Under ultrasound guidance, pericardial puncture and drainage were successfully performed. The patient's symptoms were alleviated, her vital signs stabilized, and follow-up outcomes were favorable. This case provides valuable reference for understanding the pathogenesis, diagnosis, and treatment of pericardial hemorrhage following acupuncture therapy, integrating both clinical practice and literature review.</p>","PeriodicalId":12414,"journal":{"name":"Frontiers in Cardiovascular Medicine","volume":"11 ","pages":"1433945"},"PeriodicalIF":2.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Frontiers in Cardiovascular Medicine
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1