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Characteristics of Antioxidative Flavonoid Glycosides in Lemon Fruit 柠檬果实中抗氧化类黄酮苷的特性
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.48
Y. Miyake, Kanefumi Yamamoto, Y. Morimitsu, T. Osawa
We investigated the antioxidative flavonoid glycosides in the peel extract of lemon fruit (Citrus limon). Six flavanon glycosides: eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, and neohesperidin, and three flavone glycosides: diosmin, 6,8-di-C-β-glucosyldiosmin (DGD), and 6-C-β-glucosyldiosmin (GD) were identified by high-performance liquid chromatography (HPLC) analysis. Their antioxidative activity was examined using a linoleic acid autoxidation system. The antioxidative activity of eriocitrin, neoeriocitrin and DGD was stronger than that of the others. Flavonoid glycosides were present primarily in the peel of lemon fruit. There was only a small difference in the content of the flavonoid glycosides of the lemon fruit juice from various sources and varieties. Lemon fruit contained abundant amounts of eriocitrin and hesperidin and also contained narirutin, diosmin, and DGD, but GD, neoeriocitrin, naringin, and neohesperidin were present only in trace amounts. The content of DGD, GD, and eriocitrin was especially abundant in lemons and limes; however, they were scarcely found in other citrus fruits. The content of flavonoid compounds in lemon juice obtained by an in-line extractor at a juice factory was more abundant than that obtained by hand-squeezing. These compounds were found to be stable even under heat treatment conditions (121°C, 15 min) in acidic solution.
研究了柠檬果皮提取物中的抗氧化类黄酮苷。采用高效液相色谱法(HPLC)鉴定了6种黄酮类苷:苦艾草苷、新苦艾草苷、narirutin、naringin、橙皮苷和新橙皮苷,以及3种黄酮类苷:diosmin、6,8-di- c -β-葡萄糖基diosmin (DGD)和6- c -β-葡萄糖基diosmin (GD)。用亚油酸自氧化体系测定了它们的抗氧化活性。甘草苦苷、新甘草苦苷和DGD的抗氧化活性较强。类黄酮苷主要存在于柠檬果皮中。不同来源和品种的柠檬汁中黄酮类苷的含量差异不大。柠檬果实中含有大量的苦皮苷和橙皮苷,也含有紫丁香苷、薯蓣皂苷和DGD,但GD、新苦皮苷、柚皮苷和新橙皮苷仅以微量存在。柠檬和酸橙中DGD、GD、ericitrin的含量尤其丰富;然而,在其他柑橘类水果中很少发现它们。在果汁厂采用在线萃取法提取柠檬汁,其类黄酮含量比手工榨取的柠檬汁高。这些化合物在酸性溶液中即使在热处理条件下(121°C, 15分钟)也是稳定的。
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引用次数: 46
Ferulic Acid Esterase Activities of Typical Intestinal Bacteria 典型肠道细菌阿魏酸酯酶活性研究
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.94
C. Nishizawa, T. Ohta, Y. Egashira, H. Sanada
Ferulic acid esterase activity arose in the ceca of rats that were fed on acid hydrolysate of refined corn bran. The main component of this hydrolysate was soluble ferulic acid arabinoxylan ester. In order to determine the relationship between the ferulic acid esterase activity and intestinal bacteria, the ferulic acid esterase activities from the four kinds of typical bacteria in the intestine were measured. Ferulic acid arabinose ester (LMW) and ferulic acid arabinoxylan ester (HMW) were used as substrates. The enzyme from Lactobacillus acidophilus exhibited the highest activity when LMW was used as a substrate. However, when HMW was used, all enzymes from these bacteria exhibited trace activities. At the same time, Bifidobacterium bifidum showed high xylanase and arabinofuranosidase activities. It was suggested that the xylanase and the arabinofuranosidase from bacteria such as B. bifidum attacked HMW and degraded it to lower molecules at first. The ferulic acid esterase from bacteria such as L. acidophilus might then act to release ferulic acid in the cecum.
饲喂精制玉米皮酸水解物的大鼠盲肠中阿魏酸酯酶活性升高。该水解产物的主要成分为可溶性阿魏酸阿拉伯木聚糖酯。为了确定阿魏酸酯酶活性与肠道细菌之间的关系,测定了肠道中4种典型细菌的阿魏酸酯酶活性。以阿魏酸阿拉伯糖酯(LMW)和阿魏酸阿拉伯木聚糖酯(HMW)为底物。当LMW作为底物时,嗜酸乳杆菌的酶活性最高。然而,当使用HMW时,这些细菌的所有酶都表现出微量活性。同时,两歧双歧杆菌具有较高的木聚糖酶和阿拉伯糖烷苷酶活性。两歧双歧杆菌等细菌的木聚糖酶和阿拉伯糖核苷酶首先攻击HMW并将其降解为低分子。来自嗜酸乳杆菌等细菌的阿魏酸酯酶可能会在盲肠中释放阿魏酸。
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引用次数: 8
Suppressive Effects of Polysaccharide Produced by Bacillus circulans on Chemical Mutagens-Induced SOS Response in Salmonella typhimurium 环状芽孢杆菌多糖对化学诱变剂诱导鼠伤寒沙门菌SOS反应的抑制作用
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.77
Y. Isobe, K. Yokoigawa, H. Kawai
The suppressing effects of polysaccharide produced by Bacillus circulans on the SOS response of Salmonella typhimurium TA 1535/pSK1002 induced by AF-2, MNNG, 4NQO, Trp-P-2, IQ and MeIQx were compared with those of commercial polysaccharides to find a new physiological value as an additive to processed foodstuffs. The native polysaccharide produced by B. circulans strongly suppressed SOS response induced by IQ and MeIQx, and the suppression was increased with increasing polysaccharide concentration. Xanthan gum, which is an acidic polysaccharide produced by Xanthomonas campestris, and carboxymethyl cellulose (CMC) did not suppress SOS response induced by mutagens.
通过比较环状芽孢杆菌多糖对AF-2、MNNG、4NQO、Trp-P-2、IQ和MeIQx对鼠伤寒沙门菌TA 1535/pSK1002的抑制作用,探索其作为加工食品添加剂的新生理价值。双歧杆菌产生的天然多糖对IQ和MeIQx诱导的SOS反应有较强的抑制作用,且抑制作用随多糖浓度的增加而增强。黄原胶(由campestris黄单胞菌产生的酸性多糖)和羧甲基纤维素(CMC)对诱变剂诱导的SOS反应没有抑制作用。
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引用次数: 2
Continuous Degradation of Sodium Alginate in Bioreactor Using Immobilized Alginate Lyase 固定化海藻酸解酶在生物反应器中连续降解海藻酸钠
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.71
Y. Matsubara, M. Inoue, K. Iwasaki
The enzymatic degradation of sodium alginate was continuously carried out to effectively produce alginate oligosaccharides using immobilized alginate lyase in a CSTR (continuous stirred tank reactor) system. The alginate lyase was immobilized onto the chitosan beads and the reaction was operated at an initial alginate concentration of 10 g l−1 at 35°C and pH 7.0 under the dilution rate of 0.77 to 1.74 h−1. The degradation products mainly consisted of di-, tri-, tetra-, penta-, and hexasaccharides with the highest conversion of 0.34, with the volumetric production rate of the total oligosaccharides dependent on the dilution rate. The production process was mathematically modeled from the basic material balance and the rate equation, and showed agreement between the simulated and experimental results. The present reactor system was found to be effective for obtaining alginate oligosaccharides with a high production rate.
在连续搅拌槽式反应器(CSTR)系统中,利用固定化海藻酸裂解酶对海藻酸钠进行连续酶解,有效地制备了海藻酸低聚糖。将海藻酸裂解酶固定在壳聚糖微球上,初始海藻酸浓度为10 g l−1,35℃,pH 7.0,稀释率为0.77 ~ 1.74 h−1。降解产物主要为二糖、三糖、四糖、五糖和六糖,转化率最高,为0.34,总寡糖的体积产率取决于稀释率。根据基本物料衡算和速率方程建立了生产过程的数学模型,模拟结果与实验结果吻合。实验结果表明,该反应器系统可有效地获得海藻酸盐低聚糖,产率高。
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引用次数: 4
Separation and Determination of Yellow Gardenia Pigments for Food and Iridoid Constituents in Gardenia Fruits by Micellar Electrokinetic Chromatography 胶束电动色谱法分离测定食用栀子黄色色素及栀子果实环烯醚萜类成分
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.54
Toshiro Watanabe, A. Yamamoto, S. Nagai, S. Terabe
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引用次数: 11
High Performance Liquid Chromatographic Determination of Pheophorbide-a and Its Related Chlorophyll Derivatives in Tea Leaves 高效液相色谱法测定茶叶中酚a及其相关叶绿素衍生物
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.80
K. Kohata, K. Hanada, H. Horie
A simple method for the extraction of tea samples and conditions of HPLC analysis of pheophorbide-a (PB-a) and its related chlorophyll derivatives was developed. Tea samples were extracted with 85% acetone (v/v) with this solution injected directly into the HPLC column. The modified HPLC procedure developed included a gradient solvent system in which solvent A (95% ethanol (v/v) containing 0.005 M sodium chloride) and solvent B (80% ethanol (v/v) containing 0.005 M sodium chloride) were the mobile phase. PB-a, its derivatives and their C-10 epimers could be clearly separated and determined within 35 min. This analytical method could be routinely used to determine low levels of PB-a content (<10 mg/100g) and its related individual chlorophyll derivatives in green teas. Hence, it is applicable to the safety and quality control of green teas.
建立了一种简便的茶叶样品提取方法和高效液相色谱法分析茶中酚A及其相关叶绿素衍生物的条件。茶叶样品以85%丙酮(v/v)提取,该溶液直接注入高效液相色谱柱。建立了以溶剂a(95%乙醇(v/v)含0.005 M氯化钠)和溶剂B(80%乙醇(v/v)含0.005 M氯化钠)为流动相的梯度溶剂体系。PB-a及其衍生物及其C-10外显体可在35 min内得到清晰的分离和测定。该分析方法可常规用于测定绿茶中微量PB-a (<10 mg/100g)及其相关单个叶绿素衍生物。因此,它适用于绿茶的安全和质量控制。
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引用次数: 21
Headspace gas analysis of volatile compounds of light and deep roasted sesame seed oil 轻烤和深烤芝麻油挥发性化合物的顶空气体分析
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.14
M. Shimoda, Yuji Nakada, Masatosi Nakashima, Y. Osajima
Volatile compounds in the headspace gas of light and deep roasted sesame seed oil were analyzed by gas chromatography and gas chromatography-mass spectrometry. The present method resulted in good reproducibility (<6.6% as a relative standard deviation) in the determination of individual volatile components. About 64 compounds, including 30 heterocyclic compounds, 7 aliphatic aldehydes, 11 ketones, and 16 miscellaneous compounds, were identified. Peak area percentages of 2-methylpropanal, 2-butenal, 2- and 3-methylbutanal, 2-propanone, 2-butanone, 3-methyl-2-butanone, 2,3-butanedione, 2- and 3-methylfuran, and 2,5-dimethylfuran, all of which could not be detected by steam distillation and column adsorptive concentration (previous method), increased in deep roasted oil. Hexanal decreased from 6.13% to 2.55% in deep roasted oil. Compared with the previous method, pyridine, thiophenes, and sulfides could be detected only by the present method, but unsaturated aliphatic aldehydes could not.
采用气相色谱法和气相色谱-质谱联用技术对轻烤和深烤芝麻油顶空气中的挥发性化合物进行了分析。本方法在测定单个挥发性成分时重复性好(相对标准偏差<6.6%)。共鉴定出64个化合物,包括30个杂环化合物、7个脂肪醛、11个酮类和16个杂类化合物。2-甲基丙烷、2-丁烯醛、2-和3-甲基丁烯醛、2-丙烷酮、2-丁酮、3-甲基-2-丁酮、2,3-丁二酮、2-和3-甲基呋喃和2,5-二甲基呋喃的峰面积百分比在深烤油中增加,这些都是蒸汽蒸馏和柱吸附浓度(以前的方法)无法检测到的。深焙油中己醛含量由6.13%降至2.55%。与之前的方法相比,该方法只能检测到吡啶、噻吩和硫化物,而不能检测到不饱和脂肪族醛。
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引用次数: 5
An On-Line Inverse Method for Estimation of Thermal Parameters Which Are Responsible to Predicting Temperature History in Food Heating/Freezing 一种预测食品加热/冷冻过程温度历史的热参数在线反演方法
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.1
Tomowo Mihori, Y. Hu, Hisahiko Watanabe
Most of the published methods for estimating temperature history during heating/cooling/freezing solid food require data on thermal properties of the product and any relevant heat transfer coefficients. However, there are some difficulties of obtaining thermal data for use in industrial heating/cooling/freezing of food. In this paper the development of a new procedure for estimating the temperature history is briefly reviewed, a procedure which does not require the knowledge of thermal data of the food being heated/frozen. This procedure collects a series of time/temperature data at a point in the food in the early stages of heating/freezing, analyzes these data to predict the thermal parameters which are responsible for heat conduction, and predicts the time/temperature relationship for the remainder of the heating/cooling/freezing phases.
大多数已发表的估算固体食品加热/冷却/冷冻过程中的温度历史的方法都需要产品的热性能和任何相关的传热系数的数据。然而,获取用于工业加热/冷却/冷冻食品的热数据存在一些困难。本文简要回顾了一种估算温度历史的新方法的发展,这种方法不需要了解被加热/冷冻食品的热数据。该程序收集食品在加热/冷冻初期某一点的一系列时间/温度数据,分析这些数据以预测负责热传导的热参数,并预测加热/冷却/冷冻剩余阶段的时间/温度关系。
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引用次数: 1
The Comparison of Pyrazine Compounds in Non-Salted Fermented Soybean Products. 无盐发酵豆制品中吡嗪类化合物的比较。
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.85
E. Sugawara, Tadahiko Suzuki, Yoshiko Yoshida
The odor components of six different non-salted fermented soybean products in different parts of the world, Natto (Japan), Thua-nao (Thailand), Pepo (Myanmar), Libi ippa (East Bhutan), Kinema (West Bhutan) and Daddawa (Nigeria) were compared. Especially, the composition of pyrazines contributing to Natto odor was examined in these products. The odor concentrates were prepared by the method using porous polymers and were analyzed by gas chromatography and gas chromatography-mass spectrometry. Pyrazines were considered to contribute to the characteristic odor of the non-salted fermented soybean products because the compounds were detected commonly in all the products except two types of Libi ippa. The total concentration of the pyrazines was lower in Libi ippa, Kinema, and Daddawa and higher in Thua-nao and Pepo when compared with that of Natto. The composition of the pyrazines depended on the products.
比较了日本纳豆(Natto)、泰国华糯(hua-nao)、缅甸佩波(Pepo)、东不丹利比伊帕(Libi ippa)、西不丹基内玛(Kinema)和尼日利亚达达瓦(Daddawa) 6种无盐发酵豆制品的气味成分。特别研究了这些产品中吡嗪类成分对纳豆气味的影响。采用多孔聚合物制备了气味浓缩物,并采用气相色谱和气相色谱-质谱联用技术对其进行了分析。认为吡嗪类化合物是造成无盐发酵豆制品特有气味的主要原因,因为除两种立比茶外,所有发酵豆制品均含有吡嗪类化合物。与纳豆相比,Libi ippa、Kinema和Daddawa的吡嗪总浓度较低,而hua-nao和Pepo的吡嗪总浓度较高。吡嗪类化合物的组成取决于产物。
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引用次数: 2
Photo-Isomerization of the Nasunin, the Major Eggplant Anthocyanins 茄子主要花青素纳苏宁的光异构化
Pub Date : 1998-02-25 DOI: 10.3136/FSTI9596T9798.4.25
K. Hayashi, H. Shinagawa, Atsuko Suzuki, A. Tsukui
It was reported that the acyl moieties of acylated anthocyanins with hydroxycinnamic acids were photo-isomerized from the trans- to the cis-isomer by light irradiation. We studied the effects of light irradiation on nasunin, p-coumaroylated anthocyanin from eggplant, and characterized the photo-isomerized anthocyanin by high performance liquid chromatography and fast atom bombardment mass spectroscopy. The photo-isomerization occurred under acidic conditions and was repressed with α-glucosylrutin that strongly absorbed ultraviolet light.
据报道,羟基肉桂酸酰基化花青素的酰基部分在光照射下由反式异构为顺式异构。研究了光照射对茄子对香豆素化花青素的影响,并利用高效液相色谱和快速原子轰击质谱对其进行了表征。光异构化反应发生在酸性条件下,并被强吸收紫外光的α-糖苷抑制。
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引用次数: 6
期刊
Food Science and Technology International, Tokyo
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