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Radiation Sensitivities of Listeria monocytogenes Isolated from Chicken Meat and Their Growth at Refrigeration Temperatures 鸡肉中单核增生李斯特菌的辐射敏感性及其在冷藏温度下的生长
Pub Date : 1997-09-30 DOI: 10.3136/FSTI9596T9798.4.184
Harsojo, D. Bánáti, Hitoshi Ito
Listeria monocytogenes were isolated in 5 lots, more than one cell in each 25-g sample of 10 lots of chicken meat, which was obtained from several different areas in Japan. From taxonomic study, the psychrotrophic type of 3 isolates grew well at 4°C on Trypticase soy agar slant, whereas 2 isolates grew poorly. Cells of all isolates were sensitive to γ-irradiation in phosphate buffer, and the D10 values obtained were 0.16 to 0.18 kGy under aerobic irradiation conditions similar to the values of salmonellae. In the chicken meat sample, the D10 value obtained was 0.42 kGy the same value as in phosphate buffer under anaerobic irradiation conditions, and the necessary dose for inactivation of L. monocytogenes was estimated to be 2 kGy in raw chicken meat below 10-4 CFU (colony forming unit) per gram. In the storage study of chicken meat which was inoculated with about 3×103 CFU per gram of L. monocytogenes, the psychrotrophic type of the isolates grew quickly at 7 to 10°C storage. However, a dose of 1 kGy was also effective to suppress the growth of L. monocytogenes at refrigeration temperatures below 10°C.
从日本不同地区的10批鸡肉的25 g样品中分离出5批单增李斯特菌,每批样品中有1个以上细胞。从分类上看,在4°C的胰蛋白酶大豆琼脂斜坡上,3株菌株生长良好,2株生长不良。所有分离株的细胞对磷酸盐缓冲液中的γ-辐照都很敏感,在好氧辐照条件下获得的D10值为0.16 ~ 0.18 kGy,与沙门氏菌相似。在鸡肉样品中,得到的D10值为0.42 kGy,与厌氧照射条件下磷酸盐缓冲液中的D10值相同,在每克10-4 CFU(菌落形成单位)以下的生鸡肉中,估计单核增生乳杆菌灭活所需的剂量为2 kGy。在接种约3×103 CFU / g单核细胞增生乳杆菌的鸡肉贮藏研究中,菌株在7 ~ 10℃贮藏条件下生长迅速。然而,在低于10°C的冷藏温度下,1 kGy的剂量也能有效抑制单核增生乳杆菌的生长。
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引用次数: 1
Manufacture of chungkuk-jang with elastase activity 具有弹性酶活性的青果酱的研制
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.251
Haeng-Ran Kim, K. Muramatsu, Y. Kanai, Tadayoshi Tanaka, M. Takeyasu, K. Kiuchi
Chungkuk-jang (hereafter referred to simply as jang), a traditional fermented soybean product in Korea, is manufactured by a process similar to that of Japanese itohiki-natto (referred to as natto). The bacteria isolated from jang were identified to belong to Bacillus subtilis (Ehrenberg) Cohn. The quality of jang manufactured with the isolated bacteria and two kinds of natto bacilli, B. subtilis (natto) KFP2 and KFP419, was evaluated to be as good in sensory evaluation except for the viscosity. Therefore, the isolated bacteria were estimated as B. subtilis (natto). In jang, soybean proteins were degraded to increase soluble and formol nitrogens. Sticky materials were produced, and natto-like flavor was emitted. Jang prepared in the laboratory showed elastase activity, and it was twice-to-three-times higher than that of the commercial product. While jang is a different product from that of natto, both products contain the common starter and elastase.
忠国酱(以下简称酱)是韩国传统的豆制品,其制作方法与日本的伊藤纳豆(以下简称纳豆)类似。经鉴定为枯草芽孢杆菌属(Ehrenberg) Cohn。用分离得到的细菌和两种纳豆芽孢杆菌(纳豆)KFP2和KFP419制备的酱除粘度外,其他感官评价均为良好。因此,分离得到的细菌估计为纳豆枯草芽孢杆菌。在jang中,大豆蛋白被降解以增加可溶性氮和甲醛氮。产生了粘性物质,并散发出类似纳豆的味道。张某在实验室制备的该产品显示出弹性酶活性,比市面上的产品高出2 ~ 3倍。虽然姜和纳豆是不同的产品,但这两种产品都含有共同的发酵剂和弹性蛋白酶。
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引用次数: 5
Effect of NaCl Penetration Rate on the Granulation and Oil-Off of the Yolk of Salted Duck Egg NaCl渗透率对咸鸭蛋蛋黄成粒和脱油的影响
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.269
K. Lai, W. Ko, T. Lai
Duck eggs treated with 1.0 N HCl up to 120 min to adjust the permeability of the shell were immersed in 26% NaCl (0-40 days) and subsequently heated at 85°C for 90 min to obtain the salted eggs in order to investigate the effect of NaCl on the granulation and oil-off of the yolk formed. During brining, the NaCl contents of the yolk and albumen were increased 2-10 fold due to HCl treatment of the shell. The oil-off ratio, defined as the ratio of the free lipid to the lipid content of the yolk, was also affected by the penetration rate and brining time. Eggs treated with 1.0 N HCl for 120 min showed reduced time required to achieve the maximum lipid content and oil-off ratio and had a lower maximum value of oil-off ratio than eggs treated with 1 N HCl for 0-80 min. The yolk of HCl-treated (1.0 N, 80 min) egg changed in appearance mealy form to be granulous at 5-10 days and to a soft gel at 15-20 days after brining. In comparison, the eggs without HCl treatment respectively required 20-25 and 40 days for these changes. This study suggests that sustained brining may result in the formation of a gel-state yolk, and the NaCl penetration rate affects only the time for the change in forms.
将鸭蛋用1.0 N HCl处理120 min以调节蛋壳的渗透性,在26% NaCl中浸泡(0 ~ 40 d),然后在85℃下加热90 min,得到咸蛋,以研究NaCl对蛋黄形成的肉质和脱油的影响。在盐水处理过程中,由于对蛋壳进行了HCl处理,蛋黄和蛋白的NaCl含量提高了2 ~ 10倍。脱油率(即蛋黄中游离脂肪与脂肪含量之比)也受渗透速率和浸泡时间的影响。与1 N HCl处理0-80 min的鸡蛋相比,1.0 N HCl处理120 min的鸡蛋达到最大脂质含量和脱油率所需的时间更短,脱油率的最大值也更低。1.0 N HCl处理80 min的鸡蛋蛋黄在5-10 d时呈粉状,颗粒状,15-20 d时呈软凝胶状。相比之下,未处理盐酸的鸡蛋分别需要20-25天和40天才能发生这些变化。本研究表明,持续盐水可能导致蛋黄形成凝胶状态,NaCl的渗透速率仅影响形态变化的时间。
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引用次数: 15
An Index of Proteolysis Degree in Ewes' Milk and Ewes' Milk Yoghurt, by Single Strains and Combinations of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, Isolated from Traditional Greek Yoghurt 单株及组合嗜热链球菌与德氏乳杆菌对羊奶及羊乳酸奶蛋白水解程度的研究保加利亚,从传统的希腊酸奶中分离
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.259
Aikaterini Georgala, E. Tsakalidou, I. Kandarakis, G. Kalantzopoulos
The proteolysis degree in ewes' milk by 5 Streptococcus thermophilus and 4 Lactobacillus delbrueckii subsp. bulgaricus strains, as well as by their 20 combinations in ewes' milk yoghurt, was determined. Samples for analysis were taken after 24 h and 5 days' storage at 4°C. Determination of proteolysis degree in the 2% and 12% TCA soluble fractions was performed by the photometric ninhydrin method. The proteolysis degree varied among the single strains and their combinations. The proteolytic activity of Lb. bulgaricus strains was greater than or at the same level as the activity determined for Str. thermophilus strains. In yoghurt, the proteolysis determined was higher than that of the single strains. Changes in the proteolysis degree were also observed between the 24-h and 5-day samples. The highest total amount of free amino acids/peptides soluble in the 2% and 12% TCA fraction, in the 24-h as well as in the 5-day samples, was produced by Str. thermophilus strain CNRZ 404, Lb. bulgaricus strain CNRZ 1159 and the combinations CNRZ 1198/CNRZ 1159, ACA-DC 116/CNRZ 1159.
5种嗜热链球菌和4种德氏乳杆菌亚种对母奶蛋白的水解程度。测定了保加利亚菌菌株及其在母羊乳酸奶中的20个组合。待分析的样品在4℃下保存24 h和5天。采用茚三酮光度法测定2%和12% TCA可溶性组分的蛋白水解度。单株和组合菌株的蛋白水解程度不同。保加利亚芽孢杆菌菌株的蛋白水解活性大于或与嗜热链球菌菌株的活性相同。在酸奶中,测定的蛋白水解率高于单一菌株。在24小时和5天的样品中也观察到蛋白质水解程度的变化。在24 h和5 d的样品中,2%和12% TCA组分中可溶的游离氨基酸/肽总量最高的是嗜热链球菌CNRZ 404、保加利亚链球菌CNRZ 1159以及CNRZ 1198/CNRZ 1159、ACA-DC 116/CNRZ 1159组合。
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引用次数: 6
Effect of Acylated Proteins on Textural Properties of Nonfat Low Calorie Set Yogurt and Lowfat Ice Cream 酰基化蛋白对脱脂低热量酸奶和低脂冰淇淋质地特性的影响
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.274
K. Farooq, Z. Haque
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引用次数: 2
Antimicrobial Activities of Extracts from Sachaline Giant Knotweed Polygonum sachalinense 沙沙林虎杖提取物的抗菌活性研究
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.290
M. Saito, Y. Kawai, K. Yamazaki, N. Inoue, H. Shinano
The antimicrobial activities of extracts from the leaves and rhizomes of Sachaline giant knotweed, Polygonum sachalinense, were examined. Of all the fractions separated using various solvents from the methanol extracts, the ethyl acetate fractions obtained from both the leaves and rhizomes had the highest antibacterial activity. Chloroform-separated fractions obtained from the rhizomes also displayed high antibacterial activity against some bacterial strains. Ethyl acetate-soluble fractions obtained from both the leaves and rhizomes showed effective growth inhibition against Bacillus brevis, Bacillus cereus, Bacillus coagulans, Curtobacterium flaccumfaciens, Staphylococcus aureus, Aeromonas hydrophila and Pseudomonas fluorescens.
研究了沙棘叶和根状茎提取物的抑菌活性。在不同溶剂分离的甲醇提取物中,从叶和根茎中分离得到的乙酸乙酯部分具有最高的抗菌活性。从根茎中提取的氯仿分离物对某些菌株也显示出较高的抗菌活性。从叶和根茎中提取的乙酸乙酯可溶组分对短芽孢杆菌、蜡样芽孢杆菌、凝固芽孢杆菌、屈折乳杆菌、金黄色葡萄球菌、嗜水气单胞菌和荧光假单胞菌均有有效的生长抑制作用。
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引用次数: 3
Antioxidative Constituents from the Aerial Part of Piper elongatum VAHL. 长叶胡椒(Piper elongatum)地上部抗氧化成分的研究。
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.285
C. Masuoka, M. Ono, Yasuyuki Ito, T. Nohara
Six aromatic compounds, asebogenin (1), 2',6'-dihydroxy-4'-methoxydihydrochalcone (2), 3-geranyl-4-methoxy-benzoic acid (3), 3-geranyl-4-hydroxybenzoic acid (4), nervogenic acid (5) and 2,2-dimethyl-6-carboxyl-8-prenyl-chromene (6) were isolated from the methanol extract of the aerial part of Piper elongatum VAHL., whose leaves are used as a folk medicine in South America. The structures of 1-6 were elucidated by MS, 1H-NMR and 13C-NMR spectroscopies, and chemical evidence. Among these compounds, 1 showed stronger antioxidative activity than that of α-tocopherol, and 4 and 5 exhibited higher activity than that of t-butyl-4-hydroxyanisole (BHA) using the ferric thiocyanate method.
从长叶胡椒(Piper elongatum VAHL)茎部甲醇提取物中分离得到6个芳香化合物,分别为asebogenin(1)、2′,6′-二羟基-4′-甲氧基二氢查尔酮(2)、3-香叶基-4-甲氧基苯甲酸(3)、3-香叶基-4-羟基苯甲酸(4)、神经原酸(5)和2,2-二甲基-6-羧基-8-戊基-铬(6)。它的叶子在南美洲被用作民间药物。其中1-6的结构通过质谱、1H-NMR和13C-NMR以及化学证据进行了鉴定。其中,1的抗氧化活性高于α-生育酚,4和5的抗氧化活性高于硫氰酸铁法提取的t-丁基-4-羟基茴香醚(BHA)。
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引用次数: 10
Effect of Sucrose Fatty Acid Ester Coating on the Ripening of Ethylene-Treated Cavendish Bananas. 蔗糖脂肪酸酯包衣对乙烯处理卡文迪什香蕉成熟的影响。
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.241
Mir Nurul Momen, Y. Tatsumi, Keisei Shimokawa
Sucrose fatty acid esters were used as coating materials to observe their effects on the ripening of ethylene-treated cavendish bananas. Bananas were coated with 2% sucrose fatty acid esters namely sucrose lauric, palmitic and stearic acid esters after 100 ppm ethylene application for 12 h and stored at 20°C. The sucrose fatty acid ester coating reduced the weight loss and extended the storage life of the bananas. Coated bananas reached close to the hue angle of 90 degrees later than non-coated bananas, i.e., delayed degreening. Non-coated bananas which ripened faster also lost their firmness earlier. Among the sucrose fatty acid esters, a 2% palmitic acid ester coating significantly delayed the ripening phenomenon of bananas. The reducing sugar content of 2% stearic acid ester-coated banana was 7.56 g/100 g and was found to be significantly higher than that of other treatments. Test panel members chose 2% lauric acid ester-coated bananas with respect to taste, sweetness and hardness.
以蔗糖脂肪酸酯为包衣材料,观察其对乙烯处理卡文迪什香蕉成熟的影响。香蕉涂上2%蔗糖脂肪酸酯,即蔗糖月桂酸酯、棕榈酸酯和硬脂酸酯,经100 ppm乙烯处理12小时,并在20℃下保存。蔗糖脂肪酸酯包衣减少了香蕉的失重,延长了香蕉的贮藏寿命。包衣香蕉比未包衣香蕉达到接近90度色相角的时间晚,即延迟去角质。成熟较快的无包皮香蕉也较早失去硬度。在蔗糖脂肪酸酯中,2%棕榈酸酯包衣能显著延缓香蕉的成熟现象。2%硬脂酸酯包衣香蕉的还原糖含量为7.56 g/100 g,显著高于其他处理。测试小组成员根据口感、甜度和硬度选择了2%月桂酸酯包覆的香蕉。
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引用次数: 6
Advances in Mathematical Modeling of Heat and Mass Transfer during Throughflow-Air Drying of Cereal Grain 谷物通流-空气干燥传热传质数学模型研究进展
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.226
S. Sokhansanj, W. Lang, D. Gu
This paper discusses recent advances in cereal drying. It focuses on mathematical modeling of convective heat and mass transfer using air as the drying medium. The mathematical modeling of air distribution follows conservation of momentum and a constituent equation relatinng the velocity of air to its static pressure. The paper presents data obtained from a series of detailed wheat drying experiments. It is shown that the existing simulation models can be improved once the thin layer drying equation is updated spatially and temporally within the bulk grain. The use of variable properties in calculating transient moisture contents and temperatures also improves the accuracy of mathematical simulations.
本文讨论了谷物干燥的最新进展。重点研究了以空气为干燥介质的对流传热传质数学模型。空气分布的数学模型遵循动量守恒和空气速度与其静压有关的组成方程。本文介绍了从一系列详细的小麦干燥试验中获得的数据。结果表明,对颗粒内部的薄层干燥方程进行时空更新,可以改进现有的模拟模型。在计算瞬态水分含量和温度时使用可变特性也提高了数学模拟的准确性。
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引用次数: 3
Changes in Carotenoids and Their Fatty Acid Esters in Banana Peel during Ripening 香蕉皮成熟过程中类胡萝卜素及其脂肪酸酯的变化
Pub Date : 1997-08-25 DOI: 10.3136/FSTI9596T9798.3.264
A. Subagio, N. Morita
Changes in carotenoids and their fatty acid esters in banana peel during ripening were investigated using a combination of an alumina column and high-performance liquid chromatography (HPLC). The level of total carotenoids decreased during the early stage of ripening but recovered again to the level of green fruit. Carotenoids belonging to both the α- and β-carotene categories decreased in the early stage of ripening, then the α-carotene category, especially lutein, increased significantly, but not the β-carotene category. Because the amount of α-cryptoxanthin was quite small, the process of hydroxylation of α-carotene to lutein, where α-cryptoxanthin was the intermediate product, seemed to occur quickly. Free lutein was easily esterified to lutein monoester and then was gradually further esterified to lutein diester; the amount of free, monoester and diester of lutein was about 20, 60 and 20% of total lutein, respectively after 4 days' storage. A small amount of isolutein, which is an epoxy of lutein, was observed and all isolutein was in the monoester form after 4 days' storage, but no diester form was detected.
采用高效液相色谱法(HPLC)研究了香蕉皮成熟过程中类胡萝卜素及其脂肪酸酯的变化。总类胡萝卜素含量在成熟前期下降,但又恢复到青果水平。α-类胡萝卜素和β-类胡萝卜素在成熟前期均呈下降趋势,α-类胡萝卜素,尤其是叶黄素含量显著升高,而β-类胡萝卜素含量不明显。由于α-隐黄质含量很少,α-胡萝卜素羟基化成叶黄素的过程似乎发生得很快,其中α-隐黄质是中间产物。游离叶黄素易酯化为叶黄素单酯,再逐渐酯化为叶黄素双酯;贮藏4 d后,游离叶黄素、单酯叶黄素和双酯叶黄素含量分别约占总叶黄素的20%、60%和20%。少量异黄酮为叶黄素的环氧树脂,保存4 d后,异黄酮均为单酯形式,未见双酯形式。
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引用次数: 19
期刊
Food Science and Technology International, Tokyo
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