Genomics最新文献

{"title":"bta-miR-224 regulates milk fat metabolism by targeting FABP4 in bovine mammary epithelial cells","authors":"Chuanchuan Wang ,&nbsp;Jinyan Zhao ,&nbsp;Xiaofang Feng ,&nbsp;Wei Zhao ,&nbsp;Ruoshuang Ma ,&nbsp;Baojun Yu ,&nbsp;Lin Xue ,&nbsp;Hua Wang ,&nbsp;Yafei Chen ,&nbsp;Juan Zhang professor ,&nbsp;Yaling Gu","doi":"10.1016/j.ygeno.2024.110955","DOIUrl":"10.1016/j.ygeno.2024.110955","url":null,"abstract":"<div><div>Milk fat is produced and secreted by the mammary gland, which is mainly regulated by diet and gene-molecule network. Therefore, understanding the molecular mechanism of milk fat synthesis is of practical significance for improving milk quality. Fatty acid-binding protein 4 (<em>FABP4</em>) is a candidate messenger RNA (mRNA) closely linked to milk fat metabolism obtained from transcriptomic analysis of mammary epithelial cells of cows in the pre-existing high- and low-milk-fat groups, and its expression pattern and function are still unclear. The qRT-PCR results depicted that <em>FABP4</em> was highly expressed in bovine mammary epithelial cells (BMECs) in breast tissues and the high milk fat group. Subsequently, the regulatory effects of <em>FABP4</em> on BMECs were analyzed by CCK8, EdU, and flow cytometry, and the results demonstrated that <em>FABP4</em> inhibited the proliferation and viability of BMECs and promoted their apoptosis. Next, the effect of <em>FABP4</em> on milk lipid metabolism was explored. pEGFP-N1-FABP4 was transfected into BMECs, and <em>FABP4</em> upregulated the expression levels of the milk lipid marker genes <em>XDH</em>, <em>PPARG</em>, and <em>ACSS2</em>, and promoted the formation of triglycerides (TGs), cholesterol, lipid droplets, and β-casein. Strong interactions between FABP4 and PPARG were identified using STRING prediction. Western blotting revealed that FABP4 interacted with PPARG to promote PPARG expression, while the opposite result was observed after interfering with FABP4. The gene regulation of microRNA (miRNA) is essential for fatty acid metabolism and synthesis. Predicted by website and combined with pre-miRNA transcriptome sequencing results, we hypothesized that <em>FABP4</em> might be the target gene of bta-miR-224. The results of the dual-luciferase reporter gene and qRT-PCR revealed that bta-miR-224 negatively regulated <em>FABP4</em> expression by targeting the 3′-UTR of <em>FABP4</em>. By exploring the function of bta-miR-224, we observed that bta-miR-224 mimics downregulated the expression of the milk fat marker genes <em>AGPAT6</em>, <em>ACSS2</em>, and <em>XDH</em> and inhibited TG synthesis and lipid droplet secretion. However, the bta-miR-224 inhibitor depicted the opposite results. In conclusion, <em>FABP4</em> plays a crucial role in regulating BMEC proliferation and differentiation. Bta-miR-224 targeting <em>FABP4</em> may promote biological processes such as TG synthesis and lipid droplet formation through PPARG, which lays a solid foundation for further analysis of the functional mechanism of milk lipid metabolism in dairy cows from a miRNA-mRNA perspective.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110955"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic benchmark of copy number variation detection tools for high density SNP genotyping arrays","authors":"M.N. van Baardwijk ,&nbsp;L.S.E.M. Heijnen ,&nbsp;H. Zhao ,&nbsp;M. Baudis ,&nbsp;A.P. Stubbs","doi":"10.1016/j.ygeno.2024.110962","DOIUrl":"10.1016/j.ygeno.2024.110962","url":null,"abstract":"<div><div>Copy Number Variations (CNVs) are crucial in various diseases, especially cancer, but detecting them accurately from SNP genotyping arrays remains challenging. Therefore, this study benchmarked five CNV detection tools—PennCNV, QuantiSNP, iPattern, EnsembleCNV, and R-GADA—using SNP array and WGS data from 2002 individuals of the DRAGEN re-analysis of the 1000 Genomes project. Results showed significant variability in tool performance. R-GADA had the highest recall but low precision, while PennCNV was the most reliable in terms of precision and F1 score. EnsembleCNV improved recall by combining multiple callers but increased false positives. Overall, current tools, including new methods, do not outperform PennCNV in precise CNV detection. Improved reference data and consensus on true positive CNV calls are necessary. This study provides valuable insights and scalable workflows for researchers selecting CNV detection methods in future studies.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110962"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome sequencing of Calonectria dianii: An important pathogen causing Eucalyptus leaf blight","authors":"QianLi Liu ,&nbsp;FeiFei Liu ,&nbsp;GuoQing Li ,&nbsp;KaiDong Liu ,&nbsp;ZhenChi Huang ,&nbsp;ShanNi Cao ,&nbsp;JunDi Zhong","doi":"10.1016/j.ygeno.2024.110967","DOIUrl":"10.1016/j.ygeno.2024.110967","url":null,"abstract":"<div><div>Eucalyptus leaf blight, caused by <em>Calonectria</em> spp., significantly impacts the global <em>Eucalyptus</em> industry. <em>Calonectria dianii</em>, as one of the predominant causal agents, poses a serious threat to <em>Eucalyptus</em> plantations in China. To enhance our understanding of its pathogenic mechanisms, we sequenced the genome of <em>C. dianii</em> RIFT 6520 using both Nanopore PromethION and Illumina NovaSeq PE150 platforms. Our analysis revealed a 61.76 Mb genome comprising 30 contigs with an N50 of 4,726,631 bp, a GC content of 49.74 %, and 10,184 predicted coding genes. Additionally, comparative genomic analysis between <em>C. dianii</em> and seven other significant plant-pathogenic <em>Calonectria</em> species was conducted. This analysis provided insights into the evolutionary relationships and adaptive mechanisms of these pathogens. Our study elucidates the genetic basis of <em>C. dianii's</em> pathogenicity and evolution, providing valuable information for future research on its molecular interactions with <em>Eucalyptus</em> and aiding in the development of precise control measures for Eucalyptus leaf blight.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110967"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the effect of long non-coding RNA maternally expressed gene 3 (lncRNA MEG3) on the transcriptome profile in cervical cancer cell lines","authors":"Solomon Arega ,&nbsp;Suchanda Dey ,&nbsp;Sunil Pani ,&nbsp;Somya Ranjan Dash ,&nbsp;Roli Budhwar ,&nbsp;Chanakya Nath Kundu ,&nbsp;Niladri Ganguly","doi":"10.1016/j.ygeno.2024.110957","DOIUrl":"10.1016/j.ygeno.2024.110957","url":null,"abstract":"<div><div>This study investigates the role of the long non-coding RNA Maternally Expressed Gene3 (lncRNA MEG3) gene in cervical cancer, as evidenced by its downregulation in cancerous cell lines. The study demonstrates the effects of the overexpression of lncRNA MEG3 in cervical cancer cell lines, particularly in C33A and CaSki. Through comprehensive analyses, including Next-Generation Sequencing (NGS), alterations in global mRNA expression were analyzed. In C33A cells, 67 genes were upregulated, while 303 genes were downregulated. Similarly, in CaSki cells, 221 genes showed upregulation and 248 genes displayed downregulation. Gene ontology and KEGG pathway analyses were conducted to gain insight into potential mechanisms. Furthermore, the study delves into gene regulatory networks, uncovering intricate interactions among genes. The RNA sequencing data were confirmed for eight genes: PAX3, EGR2, ROR1, NRP1, OAS2, STRA6, CA9, and EDN2 by Real-time PCR. The findings illuminate the complex landscape of gene expression alterations and pathways impacted by the overexpression of lncRNA MEG3. The impact of MEG3 on the overall cervical cancer cells' mRNA profile is reported for the first time. New biomarkers for the prognosis of cervical cancer are also reported in this study. Moreover, identifying specific genes within the regulatory networks provides valuable insights into potential therapeutic targets for managing cervical cancer.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110957"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of cell-mediated immune responses in dairy bulls via long non-coding RNAs from submandibular lymph nodes, peripheral blood, and the spleen","authors":"Xiuxin Zhao ,&nbsp;Xiao Wang ,&nbsp;Guanghui Xue ,&nbsp;Yundong Gao ,&nbsp;Yuanpei Zhang ,&nbsp;Yanqin Li ,&nbsp;Yachun Wang ,&nbsp;Jianbin Li","doi":"10.1016/j.ygeno.2024.110958","DOIUrl":"10.1016/j.ygeno.2024.110958","url":null,"abstract":"<div><div>Cell-mediated immune responses (CMIRs) are critical to building a robust immune system and reducing disease susceptibility in cattle. Long non-coding RNAs (lncRNAs) regulate various biological processes. However, to the best of our knowledge, the characterization and functions of lncRNAs and their regulations on the bovine CMIR have not been investigated comprehensively. In this study, experimental bulls were immunized with heat-killed preparation of <em>Candida albicans</em> (HKCA) to induce delayed-type hypersensitivity (DTH). Three bulls were classified as high- CMIR responders and three were low-CMIR responders, based on their classical DTH skin reactions. LncRNAs were identified in the submandibular lymph nodes, peripheral blood, and spleen of high- and low-CMIR animals using strand-specific RNA sequencing. A total of 21,003 putative lncRNAs were identified across tissues, and 420, 468, and 599 lncRNAs were differentially expressed between the two groups in the submandibular lymph node, peripheral blood, and spleen tissues, respectively. Functional analysis of the differentially expressed lncRNA (DElncRNA) target genes showed that a number of immune-related Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were enriched, including immune response, cell adhesion, nucleosome, DNA packaging, antigen processing and presentation, and complement and coagulation cascades. Tissue specificity analysis indicated that lncRNA transcripts have stronger tissue specificity than mRNA. Furthermore, an interaction network was constructed based on DElncRNAs and DEGs, and 11, 14, and 11 promising lncRNAs were identified as potential candidate genes influencing immune response regulation in submandibular lymph nodes, peripheral blood, and spleen tissues, respectively. These results provide a foundation for further research into the biological functions of lncRNAs associated with bovine CMIR and identify candidate lncRNA markers for cell-mediated immune responses.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110958"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142618326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic evolution and patterns of horizontal gene transfer in Papilio","authors":"Jiajia Wang,&nbsp;Yunfei Wu,&nbsp;Linxin Zhu,&nbsp;Kaixin Guo,&nbsp;Shichen Gao,&nbsp;Yan Dong","doi":"10.1016/j.ygeno.2024.110956","DOIUrl":"10.1016/j.ygeno.2024.110956","url":null,"abstract":"<div><div>The Papilio genus, known for its ecological and phenotypic diversity, is a valuable model for evolutionary studies. This study conducted a comparative genomic analysis of 11 <em>Papilio</em> species, revealing species-specific gene family expansions, including the UDP-glucosyltransferase 2 gene associated with insect detoxification, particularly expanding in <em>Papilio polyxenes</em>. Our analysis also revealed 199 horizontal gene transfer (HGT) acquired genes from 76 microbial species, with <em>Pseudomonadota</em> and <em>Bacillota</em> as common HGT donors across these genomes. Furthermore, we examined the evolutionary patterns of nine ABC transporter subfamilies, uncovering potential links between gene family evolution and environmental adaptation. This study provides new insights into evolutionary relationships and genomic adaptations within the <em>Papilio</em> genus, contributing to broader butterfly evolutionary research.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110956"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142618324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of heat stress-related genomic regions by genome-wide association study in Solanum tuberosum","authors":"Alexander Kaier ,&nbsp;Selina Beck ,&nbsp;Markus Ingold ,&nbsp;José María Corral ,&nbsp;Stephan Reinert,&nbsp;Uwe Sonnewald,&nbsp;Sophia Sonnewald","doi":"10.1016/j.ygeno.2024.110954","DOIUrl":"10.1016/j.ygeno.2024.110954","url":null,"abstract":"<div><div>The climate crisis impairs yield and quality of crucial crops like potatoes. We investigated the effects of heat stress on five morpho-physiological parameters in a diverse panel of 178 potato cultivars under glasshouse conditions. Overall, heat stress increased shoot elongation and green fresh weight, but reduced tuber yield, starch content and harvest index. Genomic information was obtained from 258 tetraploid and three diploid cultivars by a genotyping-by-sequencing approach using methylation-sensitive restriction enzymes. This resulted in an enrichment of sequences in gene-rich regions. Population structure analyses using genetic distances and hierarchical clustering revealed strong kinship but weak overall population structure cultivars. A genome-wide association study (GWAS) was conducted with a subset of 20 K stringently filtered SNPs to identify quantitative trait loci (QTL) linked to heat tolerance. We identified 67 QTL and established haploblock boundaries to narrow down the number of candidate genes. Additionally, GO-enrichment analyses provided insights into gene functions. Heritability and genomic prediction were conducted to assess the usability of the collected data for selecting breeding material. The detected QTL might be exploited in marker-assisted selection to develop heat-resilient potato cultivars.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110954"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between salivary microbiota and tacrolimus pharmacokinetic variability in kidney transplant","authors":"Xuyu Xiang ,&nbsp;Yi Zhu ,&nbsp;Tianyin Wang ,&nbsp;Peng Ding ,&nbsp;Ke Cheng ,&nbsp;Yingzi Ming","doi":"10.1016/j.ygeno.2024.110952","DOIUrl":"10.1016/j.ygeno.2024.110952","url":null,"abstract":"<div><div>Kidney transplantation (KT) serves as a highly effective treatment for end-stage renal disease (ESRD). Nonetheless, the administration of tacrolimus, a commonly used immunosuppressant in KT, faces challenges due to the lack of dependable biomarkers for its efficacy and the considerable variability in tacrolimus pharmacokinetics (TacIPV). In this study, 183 saliva samples from 48 KT recipients under tacrolimus therapy, alongside 9 healthy control samples, were subjected to 16S rRNA sequencing. The analysis revealed significant differences in the composition of salivary microbiota among KT recipients, patients with ESRD, and healthy controls. Moreover, trough blood concentrations (C₀) of tacrolimus were associated with alterations in microbiota composition. Notably, <em>Capnocytophage</em> consistently exhibited a negative correlation in both group-level and individual trends. Furthermore, distinct taxa were identified that effectively distinguished recipients with varying TacIPV, as demonstrated by a cross-validation random forest model (mean AUC = 0.7560), with <em>Anaerolinea</em> emerging as a prominent contributor to the classifier. These findings suggest that salivary microbiota is closely linked to tacrolimus C₀ levels and could aid clinicians in differentiating KT recipients based on TacIPV.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110952"},"PeriodicalIF":3.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142462530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of small RNAs in the spleen of MASH in a non-human primate model","authors":"Juan Zhao ,&nbsp;Yuelei Zhao ,&nbsp;Hongyu Qin ,&nbsp;Yun Ye ,&nbsp;Liwei Zhang ,&nbsp;Ruike Ding ,&nbsp;Wenbin Cao ,&nbsp;Yanru Zhang ,&nbsp;Chenjing Duan ,&nbsp;Haoze Leng ,&nbsp;Yandong Li ,&nbsp;Bo Wang ,&nbsp;Liangshuo Hu ,&nbsp;Enqi Liu ,&nbsp;Pengxiang Qu","doi":"10.1016/j.ygeno.2024.110953","DOIUrl":"10.1016/j.ygeno.2024.110953","url":null,"abstract":"<div><div>Metabolic dysfunction-associated steatotic liver disease (MASLD) and its advanced stage, metabolic dysfunction-associated steatohepatitis (MASH), are increasingly recognized as a global health issue. This study examines the role of small RNAs in the spleen of MASH using a non-human primate model. We performed high-throughput small RNA sequencing on spleen tissues from MASH-primates, revealing significant alterations in the expression of small non-coding RNAs, especially miRNAs. Notably, miR-96, miR-182, miR-183, and miR-122 showed differential expression in MASH spleens. Predictive and validation studies have identified potential target genes, such as PTX3 and NFIX, that were significantly dysregulated in spleens of MASH. These findings characterized small RNAs in spleen of MASH and offer a novel insight for further research for MASH.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110953"},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142462531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA","authors":"Wei Cong,&nbsp;Jingfu Sun,&nbsp;Zhanyu Hao,&nbsp;Maosong Gong,&nbsp;Jianing Liu","doi":"10.1016/j.ygeno.2024.110943","DOIUrl":"10.1016/j.ygeno.2024.110943","url":null,"abstract":"<div><div>THCA (Thyroid carcinoma) is the most common endocrine malignancy in the world. The PLOD1 is highly expressed in THCA, but the mechanism is still unclear. It is found that the cell proliferation and migration were inhibited in si-PLOD1 group, and promoted with PLOD1 overexpression. MAZ is the transcription factor of PLOD1. The cell activities induced MAZ were reversed by si-PLOD1. The Glucose uptake, lactate production and ATP/ADP ratio were decreased with si-PLOD1. The glycolysis related proteins GLUT1, HK2, PFKP, PKM2, LDHA and Wnt/β-catenin pathway proteins WNT5A, cyclin D1, β-catenin were inhibited, GSK-3β is increased in si-PLOD1 group. BML-284 could reversed the si-PLOD1 effects on cell activities and Wnt/β-catenin pathway. The tumor xenografts were inhibited in si-PLOD1 group. As a potential therapeutic target, PLOD1 is regulated by MAZ in THCA. PLOD1 depletion could inhibit THCA cell proliferation and metastasis by glycolysis, which is inhibited by Wnt/β-catenin pathway in THCA.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 6","pages":"Article 110943"},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142462529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}