Genomics最新文献_第5页

Key role of CYP17A1 in Leydig cell function and testicular development in Qianbei Ma goats CYP17A1 在黔北麻山羊的精原细胞功能和睾丸发育中的关键作用

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110937

Tang Wen , Zhang Yuan , Wang Zhong , Guo Wei , Chen Jiajing , Ji Quan , Wang Yanfei , Li Ruiyang , Xu Houqiang , Chen Xiang

Reproductive traits are vital economic parameters in goat production, and boosting the reproductive capacity of breeding rams is crucial for enhancing the profitability of goat farming. Currently, research on the reproductive performance of Qianbei Ma goats mainly centers on investigating mechanisms associated with prolificacy and estrous ovulation in ewes, with limited emphasis on ram reproductive aspects. This study used scanning electron microscopy and enzyme-linked immunosorbent assay (ELISA) to profile the morphology of testis and the dynamic changes of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), and Testosterone (T) in serum at different developmental stages of Qianbei Ma goats. Meanwhile, transcriptome sequencing technology was used to investigate the mRNA expression patterns in testicular tissues at different developmental stages: newborn (0 M), puberty (6 M), sexual maturity (12 M), and physical maturity (18 M). The results showed that the diameter, circumference, and area of the testicular seminiferous tubules gradually increased with age. The levels of T and LH in serum significantly increased from 0 to 6 months after birth (p < 0.05), followed by a stabilization of T levels and a significant decrease in LH levels (p < 0.05). Meanwhile, FSH shows a decreasing trend between 0 and 18 months after birth. A total of 26,437 differentially expressed genes were identified in 6 comparison groups, which involve various biological processes such as immunity, growth, metabolism, development, and reproduction, and are significantly enriched in signaling pathways related to testicular development and spermatogenesis. WGCNA analysis identified 6 regions significantly associated with testicular development and spermatogenesis, and selected 320 genes for constructing a PPI network. Ten candidate genes related to testicular development and spermatogenesis were identified, including TP53, PLK4, RPS9, PFN4, ACTB, CYP17A1, GPX4, CLDN1, AMH and DHH. Of these, the CYP17A1 gene promotes interstitial cell proliferation, and promotes T synthesis. This study provides a theoretical basis and data support for promoting efficient breeding of goats and early breeding of excellent male goats.

繁殖性状是山羊生产中至关重要的经济参数，提高种公羊的繁殖能力对提高山羊养殖业的收益至关重要。目前，对黔北麻山羊繁殖性能的研究主要集中在母羊多产和发情排卵相关机制的研究上，对公羊繁殖方面的研究较少。本研究利用扫描电镜和酶联免疫吸附试验（ELISA）分析了黔北麻羊不同发育阶段睾丸的形态以及血清中促黄体素（LH）、促卵泡激素（FSH）和睾酮（T）的动态变化。同时，利用转录组测序技术研究了黔北麻羊不同发育阶段（初生（0 M）、青春期（6 M）、性成熟（12 M）和体成熟（18 M））睾丸组织中 mRNA 的表达模式。结果显示，随着年龄的增长，睾丸曲细精管的直径、周长和面积逐渐增大。从出生后 0 个月到 6 个月，血清中的 T 和 LH 水平明显升高（p

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引用次数: 0

High-resolution dissection of human cell type-specific enhancers in cis and trans activities 人类细胞类型特异性增强子在顺式和反式活动中的高分辨率解剖。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2025.110985

Meng Wang, Xiaoxu Yang, Qixi Wu

The spatiotemporal-specific gene expression is regulated by cell type-specific regulatory elements. Here we selected the H3K4me1-associated DNA sequences as candidate enhancers in two different human cell lines and performed ChIP-STARR-seq to quantify the cell-type-specific enhancer activities with high-resolution. We investigated how the activity landscape of enhancers would change when transferred from native cells (cis activity) to another cell lines (trans activity). We obtained enhancers cis activity maps and trans activity maps in two different cell lines. The cis and trans activity maps enabled us to identify cell type-specific active enhancers, with enrichment of motifs of differentially expressed TFs. Comparisons between the cis and trans activity maps revealed general consistent regulatory property with different levels of activity in two cell lines, suggesting sequence intrinsic regulatory properties remain similar in different types of cells. This study provides a new perspective on sequence intrinsic enhancer activities in different types of cells.

时空特异性基因表达受细胞类型特异性调控元件的调控。在这里，我们选择了h3k4me1相关的DNA序列作为两种不同人类细胞系的候选增强子，并使用ChIP-STARR-seq以高分辨率量化细胞类型特异性增强子的活性。我们研究了当增强子库从原生细胞（顺式活性）转移到其他细胞系（反式活性）时，增强子库的活性景观将如何变化。我们在两种不同的细胞系中获得了顺式活性图和反式活性图。顺式和反式活性图谱使我们能够识别细胞类型特异性活性增强子，并富集差异表达tf的基序。顺式和反式活性图谱的比较显示，在两种细胞系中，不同活性水平的序列内在调控特性大致一致，这表明序列内在调控特性在不同类型的细胞中保持相似。本研究为研究序列内在增强子在不同类型细胞中的活性提供了新的视角。

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引用次数: 0

Expansion of peripheral cytotoxic CD4+ T cells in Alzheimer's disease: New insights from multi-omics evidence 阿尔茨海默病外周血细胞毒性CD4+ T细胞的扩增：来自多组学证据的新见解

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110976

Jiongxue Chen , Jiatian Xie , Fuyin Deng , Jinhua Cai , Sitai Chen , Xingrong Song , Shangzhou Xia , Qingyu Shen , Xinying Guo , Yamei Tang

The significance of the adaptive immune response in Alzheimer's disease (AD) is increasingly recognized. We analyzed scRNA-Seq data from AD patients, revealing a notable rise in CD4 cytotoxic T cells (CD4-CTLs) in peripheral blood mononuclear cells (PBMCs), validated in vivo and in vitro. This rise correlates with cognitive decline in AD patients. We also identified transcription factors TBX21 and MYBL1 as key drivers of CD4-CTL expansion. Further analyses indicate these cells are terminally differentiated, showing clonal expansion, metabolic changes, and unique communication patterns. Mendelian randomization identified risk genes SRGN and ITGB1, suggesting their genetic regulation in CD4-CTLs may contribute to AD. To summarize, our findings characterize the expansion of CD4-CTLs in the PBMCs of AD patients, providing valuable understanding into the possible mechanisms involved in the expansion of CD4-CTLs in AD.

适应性免疫反应在阿尔茨海默病（AD）中的重要性越来越被认识到。我们分析了来自AD患者的scRNA-Seq数据，揭示了外周血单核细胞（PBMCs）中CD4细胞毒性T细胞（CD4- ctl）的显著升高，这在体内和体外都得到了验证。这种上升与阿尔茨海默病患者的认知能力下降有关。我们还发现转录因子TBX21和MYBL1是CD4-CTL扩增的关键驱动因素。进一步的分析表明，这些细胞是终末分化的，表现出克隆扩增、代谢变化和独特的通讯模式。孟德尔随机化鉴定出风险基因SRGN和ITGB1，提示它们在cd4 - ctl中的遗传调控可能与AD有关。总之，我们的研究结果表征了AD患者PBMCs中cd4 - ctl的扩增，为AD中cd4 - ctl扩增的可能机制提供了有价值的理解。

引用次数: 0

Evolutionary dynamics of repetitive elements and their relationship with genome size in Acrididae Acrididae重复元件的进化动力学及其与基因组大小的关系。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110971

Lina Zhao , Hao Yuan , Xuanzeng Liu , Huihui Chang , Xuan Jing , Yimeng Nie , Yuan Huang

It is widely accepted that repetitive elements (REs) represent the primary mechanism driving genome size variation across eukaryotes. The observed genome sizes and REs of 59 species within the Acrididae were obtained and characterized. The genome sizes observed ranged from 6.60 pg to 19.35 pg, while the proportion of REs varied from 57.92 % to 83.58 %. The primary contributors were identified as LTR (2.34 % ∼ 20.98 %) and LINEs (6.70 % ∼ 16.33 %). The results of ancestral reconstruction indicated that the proportion of REs in ancestral nodes was 69.53 %, which suggests that they have undergone extensive genome expansion or contraction. A significant positive correlation was identified between the proportion of REs and genome size. Transposable elements were found to account for approximately 41 % of the observed variation in genome size. Moreover, the LTR was identified as the most significant RE type in relation to genome size expansion within the Acrididae.

人们普遍认为，重复元件（REs）代表了真核生物基因组大小变化的主要机制。获得了59种Acrididae的基因组大小和REs，并对其进行了表征。基因组大小为6.60 pg ~ 19.35 pg， REs比例为57.92 % ~ 83.58 %。主要贡献者为LTR（2.34 % ~ 20.98 %）和LINEs（6.70 % ~ 16.33 %）。祖先重建结果表明，REs在祖先节点中的比例为69.53 %，表明它们经历了广泛的基因组扩增或收缩。REs比例与基因组大小呈显著正相关。发现转座因子占观察到的基因组大小变异的约41% %。此外，LTR被认为是与Acrididae基因组大小扩展相关的最显著的RE类型。

引用次数: 0

Rapid sequencing and identification for 18-STRs long amplicon panel using portable devices and nanopore sequencer 利用便携式仪器和纳米孔测序仪对18 strs长扩增子面板进行快速测序和鉴定。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110970

Jiarong Zhang , Tingting Yang , Zihan Xie , Zilin Ren , Linyu Shi , Jiang-wei Yan , Ming Ni

STRs are the most commonly used forensic genetic markers for human identification. Nanopore sequencing has shown the advantages of high portability and large data throughput. Previous studies indicate it has great potential for profiling STRs based on the ligation library preparation method. However, this method, which requires more library preparation time and operations, is unsuitable for rapid STR profiling, particularly for field forensic applications. The transposase-based rapid library preparation method offers the possibility to perform human identification using portable instruments. However, the amplicons of conventional STR panels are too small and would be cut into scraps with rapid methods, making them impractical for genotyping. In this study, we developed an 18-STRs multiplex amplification panel with amplicons of ∼1.4 Kbp. The PCR conditions were optimized to be finished within 2 h and 12 min, and the PCR products could undergo rapid methods that involved random fragmentation. We found that, on average, 29.16 % of reads from the long-amplicon panel and rapid library kit covered the whole STR region, sufficient for downstream STR profiling analysis. We conducted a small validation experiment on 24 samples using portable instruments powered by a 1.5 kW‧h portable power source. The entire process took 10.5 h and we obtained enough data from 24 samples to perform trustworthy pairwise identification analysis using the STR profiles. The overall accuracy of the analysis was 95.36 %. In sum, the study evaluated and demonstrated the viability and potential of nanopore sequencing for forensic application in the field.

STRs是人类身份鉴定中最常用的法医遗传标记。纳米孔测序显示出高便携性和大数据吞吐量的优点。以往的研究表明，基于连接文库制备方法分析STRs具有很大的潜力。然而，这种方法需要更多的库准备时间和操作，不适合快速STR分析，特别是用于现场取证应用。基于转座酶的快速文库制备方法提供了使用便携式仪器进行人体鉴定的可能性。然而，传统STR面板的扩增子太小，并且会被快速方法切割成碎片，使其无法用于基因分型。在这项研究中，我们开发了一个18 strs多路放大面板，放大倍数为~1.4 Kbp。优化后的PCR条件在2 h和12 min内完成，PCR产物可以采用随机片段的快速扩增方法。我们发现，从长扩增子面板和快速文库试剂盒中平均有29.16 %的reads覆盖了整个STR区域，足以进行下游STR分析。我们使用1.5 kW·h移动电源供电的便携式仪器对24个样品进行了小型验证实验。整个过程耗时10.5 h，我们从24个样本中获得了足够的数据，可以使用STR配置文件进行可靠的两两识别分析。分析的总体准确度为95.36 %。总之，该研究评估并证明了纳米孔测序在法医领域应用的可行性和潜力。

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引用次数: 0

Transcriptome and metabolome revealed the effects of hypoxic environment on ovarian development of Tibetan sheep 转录组学和代谢组学揭示了缺氧环境对藏羊卵巢发育的影响。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110973

Dan Zhang , Chao Yuan , Xuejiao An , Tingting Guo , Zengkui Lu , Jianbin Liu

Many studies on the adaptability of Tibetan sheep to hypoxia have been reported, but little attention has been paid to the reproduction of Tibetan sheep living at an altitude of more than 4000 m. In this study, the ovaries of Alpine Merino sheep (AM) living in middle-high altitude areas (2500 m) and the ovaries of Gangba Tibetan sheep (GB) and Huoba Tibetan sheep (HB) living in ultra-high altitude areas (4400 m or more) were collected. Through morphological, transcriptomics and metabolomics, the effects of ultra-high altitude areas on Tibetan sheep ovarian development and the molecular mechanism of sheep's adaptability to ultra-high altitude environment were explored. The results showed that the number of granulosa cells in AM was significantly higher than that in GB and HB. The transcriptome revealed several genes related to follicular development, such as DAPL1, IGFBP1, C5, GPR12, STRA6, BMPER, etc., which were mainly enriched in related pathways such as cell growth and development. Through metabolomics analysis, it was found that the differential metabolites between the three groups of sheep were mainly lipids and lipid-like small molecules, such as Glycerol 3-Phosphate, PC (16: 0 / 18: 3 (9Z, 12Z, 15Z)), mainly enriched in lipid metabolism and other related pathways. The results of combined analysis showed that Tryptophan metabolism and Steroid hormone biosynthesis may have a significant effect on Tibetan sheep follicular development. Some genes (including HSD17B7, CYP11A1, CYP19, HSD3B1, CYP17, etc.) and some metabolites (including Cortisone, 2-Methoxyestrone, etc.) are enriched in these pathways, regulating ovarian and follicular development by affecting estrogen, progesterone, etc.. The results further revealed the molecular mechanism of Tibetan sheep to adapt to the ultra-high altitude environment and maintain normal ovarian and follicular development through the regulation of genes and metabolites.

关于藏羊对缺氧适应性的研究已有很多报道，但对海拔4000 m以上的藏羊的繁殖研究却很少。本研究采集了生活在中高海拔地区（2500 m）的高山美利奴羊（AM）的卵巢，以及生活在超高海拔地区（4400 m及以上）的岗巴藏羊（GB）和火巴藏羊（HB）的卵巢。通过形态学、转录组学和代谢组学研究，探讨超高海拔地区对藏羊卵巢发育的影响，以及对超高海拔环境适应的分子机制。结果表明，AM组颗粒细胞数量明显高于GB组和HB组。转录组揭示了几个与卵泡发育相关的基因，如DAPL1、IGFBP1、C5、GPR12、STRA6、BMPER等，这些基因主要富集在细胞生长发育等相关通路中。通过代谢组学分析发现，三组绵羊的差异代谢物主要是脂质和类脂小分子，如甘油3-磷酸、PC （16:0 / 18:3 (9Z、12Z、15Z)）等，主要富集于脂质代谢等相关途径。综合分析结果表明，色氨酸代谢和类固醇激素的生物合成可能对藏羊卵泡发育有显著影响。一些基因（包括HSD17B7、CYP11A1、CYP19、HSD3B1、CYP17等）和一些代谢物（包括可的松、2-甲氧基酮等）在这些通路中富集，通过影响雌激素、孕激素等调节卵巢和卵泡发育。结果进一步揭示了藏羊通过基因和代谢物调控适应超高海拔环境，维持卵巢和卵泡正常发育的分子机制。

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引用次数: 0

SlAN2 overexpression improves cold resistance in tomato (Solanum lycopersicum L.) by regulating glycolysis and ascorbic acid metabolism 通过调节糖酵解和抗坏血酸代谢，过表达 SlAN2 可提高番茄（Solanum lycopersicum L.）的抗寒性。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110978

Minghui Ye , Deying Wang , Ruixin Li , Kunyang Zhuang , Hongjiao Wang , Xinyin Cao , Tengfei Qin , Hengjia Zhang , Shangjing Guo , Bingjie Wu

Chilling stress seriously affects the growth and yield of tomato. Anthocyanin is a typical chilling-induced metabolite with strong antioxidant activity and photoprotective capacity. Here, we found that anthocyanin was also involved in ascorbic acid biosynthesis and glycolysis under chilling stress. SlAN2 is an important positive gene in anthocyanin biosynthesis. The results of physiological indicators showed that SlAN2 overexpression lines (A189) had a greater ability to tolerate cold stress than wild-type (WT) plants. Conjoint analysis of transcriptomics and metabonomics of A189 lines and WT plants was used to analyze the metabolic difference and the cold resistance mechanisms caused by anthocyanin under chilling stress. The anthocyanin accumulated more in A189 than that in WT under chilling stress at 4 °C for 24 h, which led to hexoses and ascorbic acid increased significantly. Results indicate that SlAN2 overexpression reduces the expression of key enzyme genes in glycolytic pathway such as phosphofructokinase (PFK) and pyruvate kinase (PK) genes, weakens glycolysis ability, and promotes accumulation of hexoses in A189 lines at 4 °C for 24 h compared with wild lines. Additionally, ascorbic acid content is increased by up-regulated the genes of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR). The increased hexose content can reduce cell osmotic potential, freezing point and synthesize more ascorbic acid, while the increased ascorbic acid content can enhance the ability to scavenge reactive oxygen species, so improves the cold resistance of tomato. The glycolysis and ascorbic acid metabolism pathway mediated by SlAN2 provides a new insight for the molecular mechanism of anthocyanins in improving the cold resistance of tomato and provides a new theoretical basis for cultivating new cold-tolerant tomato varieties.

寒冷胁迫严重影响番茄的生长和产量。花青素是一种典型的冷胁迫诱导代谢产物，具有很强的抗氧化活性和光保护能力。在这里，我们发现花青素还参与了冷胁迫下抗坏血酸的生物合成和糖酵解。SlAN2 是花青素生物合成过程中的一个重要阳性基因。生理指标结果表明，SlAN2过表达株系（A189）比野生型（WT）植株具有更强的耐寒胁迫能力。通过对A189株系和WT株系的转录组学和代谢组学的联合分析，分析了寒冷胁迫下花青素引起的代谢差异和抗寒机制。结果表明，在4 °C的寒冷胁迫下24 h，A189株系的花青素积累高于WT株系，导致己糖和抗坏血酸显著增加。结果表明，与野生品系相比，SlAN2 过表达会降低糖酵解途径中关键酶基因（如磷酸果糖激酶（PFK）和丙酮酸激酶（PK）基因）的表达量，削弱糖酵解能力，并促进 A189 品系在 4 ℃ 胁迫 24 小时后己糖的积累。此外，抗坏血酸过氧化物酶（APX）和脱氢抗坏血酸还原酶（DHAR）基因的上调也增加了抗坏血酸的含量。己糖含量的增加可降低细胞渗透势和冰点，合成更多的抗坏血酸，而抗坏血酸含量的增加可增强清除活性氧的能力，从而提高番茄的抗寒性。SlAN2 介导的糖酵解和抗坏血酸代谢途径为花青素提高番茄抗寒性的分子机制提供了新的认识，为培育耐寒番茄新品种提供了新的理论依据。

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引用次数: 0

Single-cell RNA sequencing reveals the heterogeneity of myofibroblasts in wound repair 单细胞RNA测序揭示了肌成纤维细胞在伤口修复中的异质性。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110982

Miaonan Liu , Xiaoxuan Liu , Jingchi Zhang , Shaocong Liang , Yan Gong , Shengjun Shi , Xiaopeng Yuan

Skin wound repair involves myofibroblasts crucial for tissue integrity. This study utilized single-cell RNA sequencing to explore myofibroblast diversity in various wound healing scenarios. Analysis of 89,148 cells from skin ulcers, keloids, and normal scars identified 13 cell clusters. Myofibroblast subcluster analysis unveiled 11 subsets, with subclusters 1 and 9 predominant in ulcers. Subcluster 1 exhibited heightened matrix metalloproteinase expression and involvement in bacterial response and angiogenesis, crucial in inflammation. Tissue validation confirmed subcluster 1 significance., while animal models supported upregulated CA12, TDO2, and IL-7R in chronic ulcers. These findings illuminate myofibroblast heterogeneity and their impact on wound healing, offering insights into potential therapeutic targets.

皮肤伤口修复涉及对组织完整性至关重要的肌成纤维细胞。本研究利用单细胞RNA测序来探索不同伤口愈合情况下肌成纤维细胞的多样性。对来自皮肤溃疡、瘢痕疙瘩和正常疤痕的89148个细胞进行分析，鉴定出14个细胞簇。肌成纤维细胞亚群分析揭示了11个亚群，其中亚群1和9在溃疡中占主导地位。亚簇1表现出基质金属蛋白酶的高表达，并参与细菌反应和血管生成，这在炎症中至关重要。组织验证证实了亚群1的显著性。而动物模型支持慢性溃疡中CA12、TDO2和IL-7R的上调。这些发现阐明了肌成纤维细胞的异质性及其对伤口愈合的影响，为潜在的治疗靶点提供了见解。

引用次数: 0

Genomic regions associated with Holstein heifer times bred to artificial insemination and embryo transfer services 与人工授精和胚胎移植服务的荷斯坦小母牛繁殖时间相关的基因组区域。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110972

Victoria Kelson , Jennifer Kiser , Kimberly Davenport , Emaly Suarez , Brenda Murdoch , Holly Neibergs

This study aimed to identify loci (p < 1 × 10⁻⁵) and gene sets (normalized enrichment score (NES) ≥ 3.0) associated with the number of times a heifer is bred to attain a successful pregnancy (TBRD) for Holstein heifers bred by artificial insemination (AI, n = 2754) or that were embryo transfer (ET, n = 1566) recipients. Eight loci were associated (p < 1 × 10⁻⁵) with TBRD in AI bred heifers and four loci were associated with TBRD in ET recipients. The gene set enrichment analysis with SNP data identified one gene set enriched (NES ≥ 3.0) with TBRD in AI bred heifers and two gene sets that were enriched with TBRD in ET recipients. The estimated pseudo-heritability for times bred to AI was 0.063 and 0.043 for ET. The identification of loci associated with embryonic loss aids in the selection of Holstein heifers with higher reproductive efficiencies that are AI bred or that are ET recipients.

本研究旨在确定人工授精（AI, n = 2754）或胚胎移植（ET, n = 1566）受体荷斯坦小母牛成功妊娠次数（TBRD）相关的基因座（p -5）和基因集（归一化富集评分（NES） ≥ 3.0）。8个基因座与人工授精母牛的TBRD相关（p -5），4个基因座与ET受体的TBRD相关。利用SNP数据对基因集进行富集分析，发现人工智能犊牛中有1个基因集富集TBRD (NES ≥ 3.0)，ET受体中有2个基因集富集TBRD。人工授精和体外授精的拟遗传率分别为0.063和0.043。鉴定与胚胎丢失相关的基因位点有助于选择人工授精或体外授精的繁殖效率更高的荷斯坦小母牛。

引用次数: 0

Single-cell RNA sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation 单细胞RNA测序揭示了驴精子发生和成熟过程中的动态转录谱。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics

Pub Date : 2025-01-01 DOI: 10.1016/j.ygeno.2024.110974

Yadan Jin, Fangdi Zhang, Ruixue Ma, Jingya Xing, Min Wang, Yujiang Sun, Guoliang Zhang

Introduction

With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.

Objectives

This study is to provide a comprehensive single-cell landscape analysis of spermatogenesis and maturation in donkeys.

Methods

In this study, we employed single-cell RNA sequencing to investigate cell composition, gene expression patterns, and regulatory roles during spermatogenesis and maturation in donkeys.

Results

The expression patterns of CDK1, CETN3, and UBE2J1 were found to be indicative of specific germ cells during donkey spermatogenesis. Additionally, the DEFB121, ELSPBP1, and NPC2 genes were specifically identified in the principal cells of the donkey epididymis.

Conclusions

We performed single-cell RNA sequencing to analyze the cellular composition and spatial distribution of donkey testis and epididymis, thereby generating comprehensive transcriptional atlases at the single-cell resolution.

随着对驴生产需求的增加，人们越来越关注驴的繁殖。然而，我们目前对驴生殖过程中精子发生和成熟的机制的理解仍然有限。目的：本研究旨在对毛驴精子发生和成熟过程进行全面的单细胞景观分析。方法：在本研究中，我们采用单细胞RNA测序来研究驴精子发生和成熟过程中的细胞组成、基因表达模式和调控作用。结果：CDK1、CETN3和UBE2J1的表达模式可指示驴精子发生过程中特定的生殖细胞。此外，在驴附睾主细胞中特异性鉴定出DEFB121、ELSPBP1和NPC2基因。结论：我们通过单细胞RNA测序分析了驴睾丸和附睾的细胞组成和空间分布，从而获得了单细胞分辨率的综合转录图谱。

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引用次数: 0