Pub Date : 1900-01-01DOI: 10.57034/2618723x-2022-04-03
M. Miroshnikov, K. T. Sultanova, M. Kovaleva, M. Akimova, M. Makarova
{"title":"Determination of reference intervals of creatinine clearance in laboratory animals","authors":"M. Miroshnikov, K. T. Sultanova, M. Kovaleva, M. Akimova, M. Makarova","doi":"10.57034/2618723x-2022-04-03","DOIUrl":"https://doi.org/10.57034/2618723x-2022-04-03","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125216138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-02-08
A. Solovieva, D. Aronov, O. Shubernetskaya, M. Kalinin, E. Moiseeva
{"title":"Express-modification of cylinder test for assessing the behavioral parameters of young and old cbrb and BALB/CJ mice","authors":"A. Solovieva, D. Aronov, O. Shubernetskaya, M. Kalinin, E. Moiseeva","doi":"10.29296/2618723x-2021-02-08","DOIUrl":"https://doi.org/10.29296/2618723x-2021-02-08","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"229 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127199966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.57034/2618723x-2022-04-06
M. V. Sergeeva, A. Pulkina, A. Shtro, K. Vasiliev, E. Romanovskaya-Romanko, A. Garshinina, A. V. Galochkina, A. Muzhikyan, J. Buzitskaya, M. Stukova
{"title":"Implementation experience of cotton rat model for respiratory syncytial virus infection and immunopathology","authors":"M. V. Sergeeva, A. Pulkina, A. Shtro, K. Vasiliev, E. Romanovskaya-Romanko, A. Garshinina, A. V. Galochkina, A. Muzhikyan, J. Buzitskaya, M. Stukova","doi":"10.57034/2618723x-2022-04-06","DOIUrl":"https://doi.org/10.57034/2618723x-2022-04-06","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114585174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.57034/2618723x-2022-04-07
E. Kushnir, M. Belopolskaya, V. Popov, M. Lovat
{"title":"Severity assessment of scientific procedures performed on laboratory animals. Theoretical and applied aspects","authors":"E. Kushnir, M. Belopolskaya, V. Popov, M. Lovat","doi":"10.57034/2618723x-2022-04-07","DOIUrl":"https://doi.org/10.57034/2618723x-2022-04-07","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129897799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-02-07
N.V. Demakova, M. Krasnova, G. A. Plisko, E. D. Semivelichenko, D. Ivkin, S. Okovityy
{"title":"Impedansometry as an estimating method of tertiary endpoint in experimental modeling of alimentary impairments","authors":"N.V. Demakova, M. Krasnova, G. A. Plisko, E. D. Semivelichenko, D. Ivkin, S. Okovityy","doi":"10.29296/2618723x-2021-02-07","DOIUrl":"https://doi.org/10.29296/2618723x-2021-02-07","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121563224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.57034/2618723x-2022-04-04
K. Shchepetkova, V. Kashuro, E. B. Shustov, E. G. Batotsyrenova, E. Krasnikova
{"title":"The effect of ethomerzol fumarate on the antioxidant system and lipid peroxidation of rat erythrocytes during mercury acetate poisoning","authors":"K. Shchepetkova, V. Kashuro, E. B. Shustov, E. G. Batotsyrenova, E. Krasnikova","doi":"10.57034/2618723x-2022-04-04","DOIUrl":"https://doi.org/10.57034/2618723x-2022-04-04","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123905832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2021-03-02
M. V. Morozova, G. Kalmykova, N. Akulova, E. Litvinova
пробиотические добавки на основе B. subtilis и B. licheniformis. В данной Abstract. С urrently, the standard for housing laboratory animals is barrier vivariums, which ensure the sterility of conditions for environment and diet. These conditions are not physiological and can be harmful to animals, since they de-prive them of the probiotic load of the cosmopolitan bacteria Bacillus spp. These bacteria are not representatives of the natural microflora inhabiting the intestines, but get into it when they are accidentally eaten, since they are not rare in the environment. In absence of Bacillus spp. source, the intestines are naturally cleared of them, i.e. bacteria Bacillus spp. , do not colonize the intestines of mammals. Currently, a number of data has been accumulated about positive action of Bacillus spp. representatives on digestion, resistance to bacterial and viral infections, reproductive health of animals and humans. Probiotic preparations for medicine and veterinary medicine have been developed based on Bacillus spp. The most com-mon probiotic food supplements are B. subtilis and B. licheniformis. In this study, we examined non-autoclaved and autoclaved diet and bedding for the presence of Bacillus spp. bacteria, as a factor affecting the health of laboratory animals. It was found that non-autoclaved diet for rodents contains 103 spores/g, and the feces of animals eating non-autoclaved diet - 10 4 spores/g. Samples of bedding, autoclaved diet and feces of animals eating autoclaved diet, did not shown any veg-etative forms or spores of Bacillus spp. PCR with primers to the 16S ribosomal RNA gene of bacteria showed the presence of bacteria in non-autoclaved diet and strong degradation of bacterial DNA in the autoclaved one. Thus, animals consuming non-autoclaved diet receive a probiotic load in the form of representatives of Bacillus spp., mainly in the spore form, while mice kept under sterile conditions are deprived of the positive effect of metabolites of the Bacillus spp. representatives. The activation of the immune system in these animals is also reduced due to the fragmentation of bacterial DNA. This study can be useful for specialist to decide on the need to maintain the sterility of the keeping of laboratory animals and the need to introduce probiotic bacterial cultures into the diet.
{"title":"Analysis of Bacillus spp. in the diet and feces of laboratory mice under barrier-housing and non-sterile conditions","authors":"M. V. Morozova, G. Kalmykova, N. Akulova, E. Litvinova","doi":"10.29296/2618723x-2021-03-02","DOIUrl":"https://doi.org/10.29296/2618723x-2021-03-02","url":null,"abstract":"пробиотические добавки на основе B. subtilis и B. licheniformis. В данной Abstract. С urrently, the standard for housing laboratory animals is barrier vivariums, which ensure the sterility of conditions for environment and diet. These conditions are not physiological and can be harmful to animals, since they de-prive them of the probiotic load of the cosmopolitan bacteria Bacillus spp. These bacteria are not representatives of the natural microflora inhabiting the intestines, but get into it when they are accidentally eaten, since they are not rare in the environment. In absence of Bacillus spp. source, the intestines are naturally cleared of them, i.e. bacteria Bacillus spp. , do not colonize the intestines of mammals. Currently, a number of data has been accumulated about positive action of Bacillus spp. representatives on digestion, resistance to bacterial and viral infections, reproductive health of animals and humans. Probiotic preparations for medicine and veterinary medicine have been developed based on Bacillus spp. The most com-mon probiotic food supplements are B. subtilis and B. licheniformis. In this study, we examined non-autoclaved and autoclaved diet and bedding for the presence of Bacillus spp. bacteria, as a factor affecting the health of laboratory animals. It was found that non-autoclaved diet for rodents contains 103 spores/g, and the feces of animals eating non-autoclaved diet - 10 4 spores/g. Samples of bedding, autoclaved diet and feces of animals eating autoclaved diet, did not shown any veg-etative forms or spores of Bacillus spp. PCR with primers to the 16S ribosomal RNA gene of bacteria showed the presence of bacteria in non-autoclaved diet and strong degradation of bacterial DNA in the autoclaved one. Thus, animals consuming non-autoclaved diet receive a probiotic load in the form of representatives of Bacillus spp., mainly in the spore form, while mice kept under sterile conditions are deprived of the positive effect of metabolites of the Bacillus spp. representatives. The activation of the immune system in these animals is also reduced due to the fragmentation of bacterial DNA. This study can be useful for specialist to decide on the need to maintain the sterility of the keeping of laboratory animals and the need to introduce probiotic bacterial cultures into the diet.","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128341151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723X-2021-01-06
Y. Gushchin
. The problem of cardiovascular system diseases treatment and prevention is still relevant. Pharmacotherapy plays an important role in the therapy, and, consequently, the development of new drugs. One of the invariable development stage is preclinical research involving laboratory animals determining Comparing the anatomical structure of humans and laboratory animals heart, we can note a different number of certain anatomical structures, such as the valves, chords and papillary muscles. but this variety can also be traced within the species. The number and location of the hollow and pulmonary veins have pronounced features. The course of the coronary arteries and their contribution to the blood supply of the myocardium parts also has its own species specifi city. As a result the blood supply type of the myocardium is also different. The left coronary artery dominates, but in rats and mice right and left coronary arteries evenly supply blood to the myocardium. In addition, animals have separate septum branches of the right coronary artery, which are not present in humans. Also, in rodents, the coronary arteries almost immediately branches off the aorta and run into the myocardium thickness, in humans they run epicardially for a long time. The histological structure of the heart is the same in all species. There are small differences, rather related to the size of the animals.The conducting system of the heart in all mammals has a single plan of structure. And the species specifi city is manifested in the variability of the location of nodes and their cellular composition. In this review, only the morphological structure is considered and physiological parameters are not discussed, since this topic requires a separate review.
{"title":"Comparative anatomy of the experimental animals and human heart","authors":"Y. Gushchin","doi":"10.29296/2618723X-2021-01-06","DOIUrl":"https://doi.org/10.29296/2618723X-2021-01-06","url":null,"abstract":". The problem of cardiovascular system diseases treatment and prevention is still relevant. Pharmacotherapy plays an important role in the therapy, and, consequently, the development of new drugs. One of the invariable development stage is preclinical research involving laboratory animals determining Comparing the anatomical structure of humans and laboratory animals heart, we can note a different number of certain anatomical structures, such as the valves, chords and papillary muscles. but this variety can also be traced within the species. The number and location of the hollow and pulmonary veins have pronounced features. The course of the coronary arteries and their contribution to the blood supply of the myocardium parts also has its own species specifi city. As a result the blood supply type of the myocardium is also different. The left coronary artery dominates, but in rats and mice right and left coronary arteries evenly supply blood to the myocardium. In addition, animals have separate septum branches of the right coronary artery, which are not present in humans. Also, in rodents, the coronary arteries almost immediately branches off the aorta and run into the myocardium thickness, in humans they run epicardially for a long time. The histological structure of the heart is the same in all species. There are small differences, rather related to the size of the animals.The conducting system of the heart in all mammals has a single plan of structure. And the species specifi city is manifested in the variability of the location of nodes and their cellular composition. In this review, only the morphological structure is considered and physiological parameters are not discussed, since this topic requires a separate review.","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129921011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2020-03-09
E. Belyaeva, Ya. Guschin
Summary. The immune system is an important part of the vital activity in the organism, because it prevents a penetration of foreign bodies and substances or it deletes them very quickly. Its elements can be found throughout in all organ systems in the form of various lymphoid formations or individual cells. The mucosa-associated lymphoid tissue (MALT) is one of the important parts of the immune system. The gut-associated lymphoid tissue (GALT) is the largest department of MALT, because about 80% of all defensive cells contact with the mucous membrane of the intestine. Some toxic effects on the organism show expression or suppression of immune responses of a body or don’t appear. So thorough lymphoid tissue analysis is a very important part in the preclinical study. The purpose of this study is to detect of existing visualization methods and research of the GALT and a selection of the most optimal methods for their usage in experiments. Some macroscopic research methods were considered, one of which was put into practice. The organs of male and female rats and rabbits were used in the study. A detailed description of visualization methods has not been preserved in literary sources, so the most optimal method to stain native intestinal preparations for macroscopic examination of intestinal associated lymphoid tissue was developed in experiments. The most informative method of the histological examination also was described and shown in this research, which one allows to study and the GALT and the intestinal mucosa. In this case, you need to select all the sections of the intestine, so that can be explored on all surface of the intestinal mucosa, and you can also examine in detail all the lymphoid formations of the intestine under a microscope. The results will help to expand the methods of visualisation and researching of the immunotoxicity of various drugs in preclinical studies. In addition, the results can be used in a detailed study of the structure of the immune system of an animal’s organism.
{"title":"The methods of visualization and research of the git-associated lymphoid tissue of laboratory animals","authors":"E. Belyaeva, Ya. Guschin","doi":"10.29296/2618723x-2020-03-09","DOIUrl":"https://doi.org/10.29296/2618723x-2020-03-09","url":null,"abstract":"Summary. The immune system is an important part of the vital activity in the organism, because it prevents a penetration of foreign bodies and substances or it deletes them very quickly. Its elements can be found throughout in all organ systems in the form of various lymphoid formations or individual cells. The mucosa-associated lymphoid tissue (MALT) is one of the important parts of the immune system. The gut-associated lymphoid tissue (GALT) is the largest department of MALT, because about 80% of all defensive cells contact with the mucous membrane of the intestine. Some toxic effects on the organism show expression or suppression of immune responses of a body or don’t appear. So thorough lymphoid tissue analysis is a very important part in the preclinical study. The purpose of this study is to detect of existing visualization methods and research of the GALT and a selection of the most optimal methods for their usage in experiments. Some macroscopic research methods were considered, one of which was put into practice. The organs of male and female rats and rabbits were used in the study. A detailed description of visualization methods has not been preserved in literary sources, so the most optimal method to stain native intestinal preparations for macroscopic examination of intestinal associated lymphoid tissue was developed in experiments. The most informative method of the histological examination also was described and shown in this research, which one allows to study and the GALT and the intestinal mucosa. In this case, you need to select all the sections of the intestine, so that can be explored on all surface of the intestinal mucosa, and you can also examine in detail all the lymphoid formations of the intestine under a microscope. The results will help to expand the methods of visualisation and researching of the immunotoxicity of various drugs in preclinical studies. In addition, the results can be used in a detailed study of the structure of the immune system of an animal’s organism.","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134013453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.29296/2618723x-2022-01-03
I. A. Lugovik, E. V. Simonova, E.A. Bernatskaya
{"title":"Approbation of the method for assessing cer ebral blood flow by ultrasound flowmetry on a model of unilateral, permanent cerebral ischemia in Wistar rats","authors":"I. A. Lugovik, E. V. Simonova, E.A. Bernatskaya","doi":"10.29296/2618723x-2022-01-03","DOIUrl":"https://doi.org/10.29296/2618723x-2022-01-03","url":null,"abstract":"","PeriodicalId":131225,"journal":{"name":"Laboratornye Zhivotnye dlya nauchnych issledovanii (Laboratory Animals for Science)","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134388251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：