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Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators 桔梗的微繁研究希恩。叶片外植体和单节对植物生长调节剂的响应
Pub Date : 2023-09-20 DOI: 10.16882/hortis.1363441
Hakan AKTAŞ, Gizem Gökçe AKKAYA, Ebru AKYÜZ ÇAĞDAŞ, Gülden HASPOLAT, Şeküre Şebnem ELLİALTIOĞLU
Lisianthus has long-lasting, white, blue-purple, velvet or pink flowers. Varieties that can be used in pots, outdoors, or cut flowers are widely used and it is essential to develop new varieties to increase their commercial value. Breeding studies with classical methods can take a long time in lisianthus. Techniques to create variation in these plants and facilitate their reproduction always contribute to shortening the breeding period and one of the best known of these methods is plant tissue culture. This study aimed to investigate the micropropagation possibilities in lisianthus and examine the effects of auxin and cytokinin applications and doses on shoot reproduction. Supplemented with 10 different plant growth regulators in Murashige and Skoog (MS) medium, leaf explants from lisianthus plants that were germinated under in vitro conditions (seedlings) were cultivated. 6-benzyl aminopurine (BAP) was utilized in 5 doses (0.5, 1.0, 2.0, 3.0, and 4.0 mg L-1) alone in the first 5 groups. Naphthalene acetic acid (NAA) was also added to the BAP mediums at a concentration of 0.5 mg L-1. Shoot formation, shoot lengths, the number of shoots longer and shorter than 1 cm, and regeneration of new shoots after subcultures were determined. According to the results, MS mediums containing 3.0 and 4.0 mg L-1 BAP could be good starting mediums for regenerating new lisianthus shoots from leaf explants. These treatments got the highest values both in shoot formation and the number of shoots longer than 1 cm. It has been determined that 3.0 mg L-1 BAP alone can be used for both organogenesis from leaf explants and shoot proliferation in single node culture. In vitro shoots of the lisianthus species were easily rooted and both PGR-free MS or ½ MS mediums. Lisianthus shoots propagated under in vitro conditions can be rooted under ex vitro conditions both in the float hydroculture and in the soil mixture. The acclimatization stage was optimized also successfully.
百合花有持久的,白色,蓝紫色,天鹅绒或粉红色的花。盆栽、户外、切花品种应用广泛,开发新品种提高其商业价值十分必要。用传统的育种方法研究麝香花可能需要很长时间。在这些植物中创造变异和促进其繁殖的技术总是有助于缩短育种周期,其中最著名的方法之一是植物组织培养。本研究旨在探讨万寿菊的微繁可能性,并探讨生长素和细胞分裂素的施用和剂量对万寿菊茎部繁殖的影响。在Murashige和Skoog (MS)培养基中添加10种不同的植物生长调节剂,培养离体萌发的百合花叶片外植体(幼苗)。前5组分别单独使用6-苄基氨基嘌呤(BAP) 0.5、1.0、2.0、3.0、4.0 mg L-1 5个剂量。在BAP培养基中加入浓度为0.5 mg L-1的萘乙酸(NAA)。测定了新梢形成、新梢长度、长、短于1cm的新梢数量以及继代培养后新梢的再生情况。结果表明,含3.0和4.0 mg L-1 BAP的MS培养基可作为百合叶片外植体再生新芽的良好起始培养基。这些处理在芽形成和长于1 cm的芽数上均最高。结果表明,3.0 mg L-1 BAP既能促进叶片外植体的器官发生,又能促进单节培养中茎部的增殖。在无pgr的MS或½MS培养基中,桔梗的离体芽都很容易生根。在离体条件下繁殖的百合花嫩芽在浮液培养和混合土中均可生根。驯化阶段的优化也取得了成功。
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引用次数: 0
Determination of Insecticide Resistance in Western Flower Thrips [Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae)] Causing Problems in Carnation Cultivation 西方花蓟马(Frankliniella occidentalis (Pergande))(蓟翅目:蓟科)对杀虫剂抗性的测定及康乃馨栽培中的问题
Pub Date : 2023-09-12 DOI: 10.16882/hortis.1359255
Musa KIRIŞIK, Tuba KAHRAMAN
The fact that Frankliniella occidentalis (Thysanoptera: Thripidae) completes its life cycle in a short time reveals the need for continuous pest control. Therefore, pest resistance may occur with the intensive use of chemicals by the growers. In this context, the current sensitivity of F. occidentalis to registered insecticides for carnation (methiocarb, formetanate hydrochloride), registered insecticides for vegetables (azadirachtin, malathion, and spinosad), and unapproved insecticides used extensively by growers needs to be determined. Moreover, it is important to test the chemicals (for example, pyridalyl) used against other pests whose spray period is the same as F. occidentalis. In this study, it was aimed to determine the sensitivity levels of F. occidentalis populations taken from the sites of intensive carnation production in Antalya province in 2018-2020 to these chemicals by the leaf dipping method. As a result of the study, resistance against spinosad (11.00-28.60 times), methiocarb (2.10-2.70 times), malathion (2.05-4.21 times), azadirachtin (3.00-7.00 times), formetanate hydrochloride (1.50-2.00 times) and pyridalyl (2.75-3.89 times) were determined. Given the high resistance to spinosad observed in the study, trials involving the combination of spinosad and formetanate hydrochloride were initiated as a strategy for managing resistance. The resistance against spinosad + formetanate hydrochloride was determined between 4.35 and 9.09 times. Our results suggest that resistance level can be reduced by using resistance management methods such as the use of mixed chemicals, although resistance was detected in all five locations against all active substances.
西方Frankliniella occidentalis(蓟翅目:蓟科)在短时间内完成其生命周期的事实表明需要持续的害虫防治。因此,随着种植者大量使用化学品,可能会出现害虫抗性。在这种情况下,需要确定西灰蝇对已登记的康乃馨杀虫剂(甲硫威、甲酸乙酯盐酸盐)、已登记的蔬菜杀虫剂(印楝素、马拉硫磷和spinosad)以及种植者广泛使用的未经批准的杀虫剂的敏感性。此外,重要的是要测试化学物质(例如吡啶)用于对付其他害虫,这些害虫的喷洒周期与西花蓟马相同。本研究旨在通过浸叶法测定2018-2020年安塔利亚省石竹集约产地的西花蓟马种群对这些化学物质的敏感程度。结果:测定其对spinosad(11.00 ~ 28.60倍)、甲硫威(2.10 ~ 2.70倍)、马拉硫磷(2.05 ~ 4.21倍)、印楝素(3.00 ~ 7.00倍)、甲酸乙酯盐酸盐(1.50 ~ 2.00倍)、吡啶醇(2.75 ~ 3.89倍)的抗性。鉴于研究中观察到的对spinosad的高耐药性,将spinosad和盐酸甲酸乙酯联合使用的试验开始作为控制耐药性的策略。对盐酸spinosad +甲酸乙酯的抗性在4.35 ~ 9.09次之间。我们的研究结果表明,尽管在所有五个地点都检测到对所有活性物质的抗性,但通过使用混合化学品等抗性管理方法可以降低抗性水平。
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引用次数: 0
Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection 利用胚胎挽救和倍性检测对柑橘砧木2x × 2x内杂交的改良
Pub Date : 2023-08-14 DOI: 10.16882/hortis.1343859
Şenay Kurt, F. Koyuncu
Diploid Citrus × Poncirus hybrids have significantly contributed to citrus rootstock evaluation. In Türkiye, common sour orange rootstock is used in many different climates and soil conditions at almost 85% of citrus plantations, but this rootstock is susceptible to Citrus tristeza virus disease. This study was conducted to improve new rootstock genotypes by traditional hybridization. Common sour orange (CSO) and Common mandarin (Citrus deliciosa Ten.) (CM) were crossed with Troyer citrange (TC) while King mandarin (KM) was crossed with Carrizo citrange (CC). Embryos obtained from crosses were taken on 110, 120 and 130 days after artificial pollination (DAP), and were germinated on MT culture media. The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%. The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos. Survival rates of the genotypes were ranged between 72.13% and 90.28% in subculture and varied from 40.17% and 64.71% in the greenhouse. As a result of the ploidy analysis by flow cytometry, the nuclear DNA content of diploid genotypes were found between 0.78 pg/2C and 0.93 pg/2C. One of the genotypes derived from CM×TC hybridization on 120 DAP was determinated as a triploid plant.
二倍体柑桔与柑桔杂交对柑桔砧木评价有显著贡献。在 rkiye,普通的酸橙砧木在许多不同的气候和土壤条件下使用,几乎85%的柑橘种植园,但这种砧木易患柑橘tristeza病毒病。本研究采用传统杂交技术改良砧木新基因型。将普通酸橙(CSO)和普通柑桔(Citrus deliciosa Ten.) (CM)与Troyer柑桔(TC)杂交,将King柑桔(KM)与Carrizo柑桔(CC)杂交。分别于人工授粉后110、120和130天取胚,在MT培养基上萌发。120 DAP组合的胚在CSO×TC内萌发率最高,为95.15%;CM×TC, 96.25%;公里×CC, 95.23%。各组合传代三叶率(CSO×TC, 17.40%;CM×TC, 11.11%;公里×CC;6.17%)。继代培养的成活率为72.13% ~ 90.28%,温室栽培的成活率为40.17% ~ 64.71%。流式细胞术倍性分析发现,二倍体基因型的细胞核DNA含量在0.78 ~ 0.93 pg/2C之间。在120株DAP上CM×TC杂交得到的一个基因型为三倍体植株。
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引用次数: 0
The Effect of the Application of Mycorrhiza on Vegetative Growth, Mineral Element Intake, and Some Biochemical Characteristics of Strawberry Seedlings under Lime Stress 施菌对石灰胁迫下草莓幼苗营养生长、矿质元素摄入及部分生化特性的影响
Pub Date : 2023-07-20 DOI: 10.16882/hortis.1330523
G. Balcı
This study aims to determine the effects of mycorrhiza (VAM) applications on vegetative growth, mineral element intake, and some biochemical characteristics of strawberry seedlings grown in lime stress conditions. The experiment was conducted in the pots filled with the lime-added mixture at the rate of 1% and peat perlite at the rate of 1:1 with frigo seeds belonging to the “Albion” strawberry cultivar. In the uprootings performed in three different stages (four-full leafed, blooming, and fruit stages) to examine the biochemical effects of mycorrhiza applications against the lime stress, vegetative growth criteria (leaf chlorophyll and anthocyanin content, area, crown diameter, fresh and dry plant weights) and mineral element contents in the plant parts (leaf, crown, and root) were determined. The proline, total phenolic amount, and lipid peroxidation (MDA) analyses were conducted on the leaf samples taken in these uprooting. In all three stages, an increase in crown diameter and leaf area was determined. In uprooting periods, proline and total phenolic amounts were increased, and, on the other hand, MDA was decreased. Microelement intake, which decreased with the lime application, was detected to be increased with mycorrhiza applications. At the end of the experiment, mycorrhiza application was observed to lessen the effect of lime stress on strawberry seedlings were exposed to.
本研究旨在研究石灰胁迫条件下菌根(VAM)对草莓幼苗营养生长、矿质元素摄入及部分生化特性的影响。试验采用“Albion”草莓品种的冷冻种子,在加石灰比例为1%和泥炭珍珠岩比例为1:1的混合料罐中进行。在三个不同阶段(四满叶期、开花期和果期)进行连根拔根,以研究菌根施用对石灰胁迫的生化影响,确定了营养生长标准(叶片叶绿素和花青素含量、面积、冠径、鲜株和干株重)和植物部位(叶、冠和根)的矿物元素含量。对连根拔叶样品进行脯氨酸、总酚含量和脂质过氧化(MDA)分析。在这三个阶段,冠径和叶面积都有所增加。在拔根期,脯氨酸和总酚含量增加,而丙二醛含量降低。微量元素的摄入量随着石灰的施用而减少,而随着菌根的施用而增加。在试验结束时,观察到施用菌根可以减轻石灰胁迫对草莓幼苗的影响。
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引用次数: 0
Determination of Phytophthora Species Causing Root and Crown Rot on Tomatoes Grown in Antalya Province and Reactions of Some Tomato Genotypes against Phytophthora nicotianae 安塔利亚地区番茄根冠腐病病原菌的测定及不同基因型番茄对烟草疫霉的反应
Pub Date : 2023-06-06 DOI: 10.16882/hortis.1310353
E. Gümrükcü, G. Karaca
Antalya province is the main center of vegetable production in Türkiye. Tomato comes first in terms of crops cultivated under protection. Phytophthora species causing root and collar rot are among the factors negatively affecting tomato yield and quality. This research aimed to determine; the prevalence of root and collar rot of tomatoes grown in Antalya province, and identify Phytophthora species causing disease. During surveys performed in 170 tomato greenhouses, plant and soil samples were taken from the areas where root and crown rot, stem blight, and drying symptoms were observed. Disease prevalence and incidence in the investigated greenhouses were 25.88% and 4.87%, respectively. Phytophthora symptoms were not observed in the greenhouses in Demre and Kepez districts, while the highest disease prevalence was found in Elmalı district with 75%. Eighty of 84 Phytophthora isolates were identified as P. nicotianae and four as P. capsici, according to their cultural, morphological and molecular features. Virulence of 18 selected isolates were determined by using stem inoculation technique and all isolates caused lesions with different lengths on tomato stems. The reactions of 22 tomato genotypes in the gene pool of BATEM against P. nicotianae were also investigated and the genotype DT-15 was found as the most susceptible genotype with the largest lesions, while A-286 was the most resistant genotype. This study formed the basis for further studies on tomato breeding and integrated disease management.
安塔利亚省是土耳其主要的蔬菜生产中心。在受保护的作物中,番茄排名第一。引起番茄根腐病和颈腐病的疫霉是影响番茄产量和品质的主要因素之一。本研究旨在确定;在安塔利亚省种植的番茄根腐病和领腐病的流行,并确定致病的疫霉种。在对170个番茄温室进行的调查中,从观察到根冠腐病、茎枯病和干燥症状的区域采集了植物和土壤样本。调查温室病害患病率和发病率分别为25.88%和4.87%。Demre和Kepez地区的温室未见疫霉症状,而elmalir地区的患病率最高,为75%。根据菌株培养、形态和分子特征,84株疫霉菌分离物中有80株鉴定为烟草疫霉菌,4株鉴定为辣椒疫霉菌。采用茎接种法测定了18株分离株的毒力,结果表明,所有分离株均对番茄茎造成不同长度的损伤。研究了BATEM基因库中22个番茄基因型对烟草疫病毒的反应,发现DT-15基因型对烟草疫病毒的易感程度最大,而A-286基因型对烟草疫病毒的抗性最强。本研究为进一步研究番茄育种和病害综合治理奠定了基础。
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引用次数: 0
Evaluation of The Relations between Yield and Yield Components of Tomato (Solanum lycopersicum L.) Hybrids by Correlation and Path Analysis 番茄产量与产量成分关系的评价杂种的相关和通径分析
Pub Date : 2023-04-21 DOI: 10.16882/hortis.1283084
I. Celik, Serkan Aydin, Halim Can Kayikçi̇, A. Ünlü, E. Gümrükcü, N. Çelik, Yıldız Doğan, S. M. Sülü
Tomato is one of the most produced vegetables in the world and there are many plant breeding studies that are carried out on this vegetables species. One of the most important aims of tomato breeding is the improvement of fruit quality and yield in both open-field and greenhouse growing conditions. The knowledge of factors with regard to yield is making plant breeders work easier. In the present study, the correlations of some plant characteristics thought to be related to yield and their direct and indirect effects on yield were analyzed. This study was conducted with 14 genotypes in 2020 and a randomized complete block design was employed as an experimental design. The relationship between 12 traits and yield was determined through path coefficient analysis. It was determined that the number of days from first fluorescence time to first fruit set time and Brix value have a directly negative effects on the yield. However, fruit length and early yield have a directly positive effect on the yield. According to the result of this study, in the correlation matrix, the number of days from first fruit set time to fruit ripening, stem length to first flower cluster, stem length, fruit diameter, leaf diameter, leaf length, average fruit yield and number of fruit per plant are insignificant. The obtained results may be utilized potential as selection criteria in the future studies about yield.
番茄是世界上产量最大的蔬菜之一,对这种蔬菜进行了许多植物育种研究。番茄育种最重要的目标之一是在露天和温室条件下提高果实品质和产量。有关产量因素的知识使植物育种者的工作更容易。本文分析了一些被认为与产量有关的植物性状的相关性及其对产量的直接和间接影响。本研究于2020年选取14个基因型,采用随机完全区组设计作为实验设计。通过通径分析确定了12个性状与产量的关系。结果表明,从第一次荧光时间到第一次坐果时间的天数和糖度值对产量有直接的负影响。而果长和早产量对产量有直接的正影响。本研究结果表明,在相关矩阵中,从第一次坐果时间到果实成熟的天数、茎长到第一次花簇的天数、茎长、果径、叶径、叶长、平均果产量和单株果数均不显著。所得结果可作为今后产量研究的选择标准。
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引用次数: 0
The Effects of Different Growing Media and Humic Acid Applications on the Growth of Tomato Plants 不同培养基及施用腐植酸对番茄植株生长的影响
Pub Date : 2023-04-15 DOI: 10.16882/hortis.1279147
Beyzanur Yildiz, Aslıhan ÇİLİNGİR TÜTÜNCÜ, S. Demirkaya, H. Özer, A. Uğur
This study was carried out to determine the effects of different growing media (peat + perlite, cocopeat, hazelnut husk, rock wool) and different humic acid doses (2, 4, and 8 ml L-1) on tomato plant growth. The performance of the seedlings from planting to fruit set was evaluated to determine the growth of the tomato plant. Fort his purpose; stem diameter (mm), plant height (cm), number of leaves, first flowering and first fruit set, total plant dry weight (g), root volume (m3), and relative growth rate (g cm-2 days-1) parameters were examined. According to the results, the highest stem diameter of 15 mm was obtained in the hazelnut husk media at a dose of 4 ml L-1 of humic acid. As a result, while the best root growth was obtained at 2ml L-1 humic acid doses in peat-perlite media, the fastest growth and N (3.33%), P (0.58%) and K (3.91%) content were obtained at 8 ml L-1 humic acid doses in cocopeat media. The best leafing, flowering and fruit set were obtained in cocopeat media with 2 ml L-1 humic acid doses. As a result, the highest relative growth rate (0.064 g cm-2 days-1) at 8 ml L-1 humic acid doses applied in the fastest growing cocopeat media.
研究了不同培养基(泥炭+珍珠岩、椰油、榛子壳、岩棉)和不同腐植酸剂量(2、4、8 ml L-1)对番茄植株生长的影响。对幼苗从种植到坐果的表现进行评价,以确定番茄植株的生长情况。为了他的目的;测定茎粗(mm)、株高(cm)、叶片数、初花和初坐果数、植株总干重(g)、根体积(m3)和相对生长率(g cm-2 days-1)等参数。结果表明,当腐植酸添加量为4 ml L-1时,榛果壳培养基的茎粗可达15 mm。结果表明,在泥炭-橄榄岩培养基中,腐植酸浓度为2ml L-1时根系生长最佳,而在鸡粪培养基中,腐植酸浓度为8ml L-1时根系生长最快,N(3.33%)、P(0.58%)和K(3.91%)含量最高。腐植酸浓度为2 ml -1的cocopin培养基的叶片、开花和坐果效果最佳。结果表明,在生长最快的腐植酸培养基中,施用8 ml L-1的腐植酸时,相对生长率最高(0.064 g cm-2天-1)。
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引用次数: 0
Assessment of Different Growing Media on Cut Flower Performance of Two Gladiolus (Gladiolus grandiflorus) Cultivars 不同培养基对两个剑兰品种切花性能的评价
Pub Date : 2023-04-15 DOI: 10.16882/hortis.1277794
Ü. Ö. Karagüzel
One of the most important problems encountered in the cultivation of cut flower gladiolus is soil-borne diseases and pests. This problem substantially reduces flower yield and quality. Soilless culture is very limited for gladiolus and it has not been studied extensively. The characteristics of the growing media used in soilless culture either directly or indirectly affect yield and quality. Therefore, it is quite essential to determine the appropriate growing media in cut flower cultivation. At the same time good flower production usually depends upon various factors including the type of growing media used. The present study was conducted to determine the effects of two different gladiolus varieties (Gladiolus grandiflorus L. cv. “Purple Flora” and “Ibadan”) and six different growing media (peat+pumice: 1:1, v/v; peat+perlite: 1:1, v/v; rice hull+pumice: 1:2, v/v; coarse sand+peat: 2:1, v/v; soil; and cocopeat) on the some quality parameters of gladiolus in in Batı Akdeniz Agricultural Research Institute (BATEM), Antalya, Türkiye. Quality parameters (stem length, flower stem diameter, stem weight, flowering time, number of florest) were significantly (p < 0.01) affected by the different growing media and cultivars. Among the growing media, the earliest flowering time (77.8 days) and the longest stem length (128.0 cm) were determined in peat+perlite, whereas the largest number of florets (15.0 florets spike-1) were recorded in peat+pumice. Regarding the varieties, Purple Flora (84.7 days) flowered earlier than Ibadan (102.7 days), while Ibadan displayed more superior characteristics in terms of the other parameters.
切花剑兰栽培中遇到的最重要的问题之一是土传病虫害。这个问题大大降低了花的产量和质量。剑兰无土栽培的应用十分有限,尚未得到广泛的研究。无土栽培中所用培养基的特性直接或间接地影响产量和品质。因此,在切花栽培中确定适宜的培养基是十分必要的。同时,良好的花卉生产通常取决于各种因素,包括所使用的生长介质的类型。本研究以两个不同的剑兰品种(gladiolus grandflorus L. cv.)为研究对象。“紫色植物”和“伊巴丹”)和六种不同的生长介质(泥炭+浮石:1:1,v/v;泥炭+珍珠岩:1:1,v/v;稻壳+浮石:1:2,v/v;粗砂+泥炭:2:1,v/v;土壤;在土耳其安塔利亚bataiakdeniz农业研究所(BATEM)对剑兰的一些品质参数进行了研究。品质参数(茎长、茎粗、茎重、开花时间、花数)受不同培养基和品种的影响极显著(p < 0.01)。在不同的培养基中,泥炭+珍珠岩的开花时间最早(77.8 d),茎长最长(128.0 cm),而泥炭+浮石的小花数最多(15.0小花穗-1)。紫色花(84.7 d)早于伊巴丹花(102.7 d),而伊巴丹花在其他参数上表现出更强的优势。
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引用次数: 0
The Use of Silver Nitrate as an Elicitor to Increase Bioactive Compounds in Artichoke [Cynara cardunculus var. scolymus (L.) Fiori] Callus Culture 硝酸银作为激发剂增加洋蓟[Cynara cardunculus vars . scolymus (L.)]生物活性物质的研究愈伤组织培养
Pub Date : 2023-04-01 DOI: 10.16882/hortis.1264849
Tuğçe Özsan Kılıç, A. Onus
The globe artichoke belongs to the Asteraceae family and has become more and more popular among other vegetables due to its beneficial health-promoting features that are related to bioactive compounds present in leaves. The plant materials have inadequate proportions of valuable bioactive compounds in nature, so researchers are emphasizing on how to enhance their amounts. In vitro techniques with integrated novel practices can be employed to enhance phytochemicals from any plant. The current study aimed to determine and assess valuable bioactive components in 3 artichoke cultivars via callus cultures which were subjected to a treatment of 4 different concentrations (2.5, 5.0, 10.0, and 15.0 mg L-1) of silver nitrate. Results indicated that well-balanced levels of plant growth regulators were necessary for stimulating the callus formation of globe artichoke. The findings of the current study also revealed the importance of cultivar differences regarding callus formation. Experimental results revealed that variation in silver nitrate concentrations had a significant effect on biomass, total phenolic content and total antioxidant activities. The results of the current, study may offer a good strategy by promoting bioactive compounds of globe artichoke leaves for utilizing in large-scale industries, pharmacology, and food supplements.
洋蓟属于菊科植物,由于其叶片中存在的生物活性化合物具有有益的促进健康的特性,在其他蔬菜中越来越受欢迎。植物材料中有价值的生物活性物质在自然界中所占的比例不足,因此如何提高其含量成为研究的重点。综合新颖实践的体外技术可用于从任何植物中提取植物化学物质。本研究旨在通过4种不同浓度(2.5、5.0、10.0和15.0 mg L-1)硝酸银处理的愈伤组织培养,确定和评估3个洋蓟品种有价值的生物活性成分。结果表明,平衡水平的植物生长调节剂是促进洋蓟愈伤组织形成的必要条件。本研究结果还揭示了愈伤组织形成的品种差异的重要性。实验结果表明,硝酸银浓度的变化对生物量、总酚含量和总抗氧化活性有显著影响。本研究结果为促进洋蓟叶生物活性物质在工业、药理学和食品添加剂等方面的大规模利用提供了良好的策略。
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引用次数: 0
Determination of Shelf-Life of New Satsuma (Rize) Mandarin (Citrus unshiu Marc.) Cultivar Candidates Obtained by Clonal Selection 新萨摩柑(Rize)柑(Citrus unshiu Marc.)保质期的测定克隆选择获得的候选品种
Pub Date : 2023-04-01 DOI: 10.16882/hortis.1264865
K. Yazıcı, Burcu GÖKSU KARAOĞLU, M. Aydın
Satsuma (Rize Mandarin) is Türkiye's most widely produced and exported mandarin variety. Due to its early ripeness and seedlessness, Satsuma mandarin (Citrus unshiu Marc.) is a popular citrus fruit in domestic and foreign markets. The present study was conducted at Recep Tayyip Erdogan University, Faculty of Agriculture, Department of Horticulture in 2020-2021 to determine the shelf-life of satsuma mandarin genotypes obtained as a result of clone selection carried out in Rize province and its districts. The harvested mandarin variety candidates were pomologically analyzed and stored in an environment containing 15°C ± 1 temperature and 55-60% humidity. The changes in fruit weight (g), fruit yield (%), rind thickness (mm), total soluble solid (%, TSS), and titratable acid (%, TA) content were examined at one-week intervals during the storage period. It was determined that weight loss (%) and TSS (%) increased steadily, while fruit juice content (%), rind thickness (mm), and titratable acid (%) decreased steadily during storage in all genotypes and control. Also, the differences detected in terms of the properties examined were lower on days 7 and 14 than the initial value, whereas they were higher on days 21. As a result of the findings, it was determined that the quality losses of mandarin genotypes showed differences during the shelf-life, but they could be stored for 14 days without much loss in quality.
萨摩(Rize Mandarin)是日本最广泛生产和出口的柑橘品种。由于其早熟和无籽,萨摩柑(Citrus unshiu Marc.)是一种受国内外市场欢迎的柑橘类水果。本研究于2020-2021年在雷杰普·塔伊普·埃尔多安大学农学院园艺系进行,以确定在里泽省及其地区进行无性系选择所获得的蜜橘基因型的保质期。对收获的柑橘候选品种进行形态学分析,并在15°C±1温度和55-60%湿度的环境中保存。每隔一周检测果实重(g)、产量(%)、果皮厚度(mm)、可溶性固形物总量(%)和可滴定酸(%)含量的变化。结果表明,在贮藏过程中,各基因型和对照的失重率(%)和TSS(%)稳步增加,果汁含量(%)、果皮厚度(mm)和可滴定酸(%)稳步下降。此外,在第7天和第14天检测到的特性差异低于初始值,而在第21天则高于初始值。结果表明,不同基因型的柑桔在贮藏期间的品质损失存在差异,但在贮藏14 d后品质损失不大。
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Horticultural Studies
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