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Identification and Toxicity Evaluation of Beauveria sp. Associated with White Muscardine Disease in Muga Silkworm Antheraea assamensis Helfer 与穆加蚕白麝病有关的 Beauveria sp.的鉴定和毒性评估 Antheraea assamensis Helfer
Pub Date : 2024-02-01 DOI: 10.55446/ije.2024.1698
Sanghamitra Saharia, Manabendra Nath, Shibani Kalita, Dimpimoni Kalita, S. Bardoloi
The present study is an effort to isolate and identify the Beauveria sp. from white muscardine infected Antheraea assamensis Helfer (muga silkworm) larvae collected from rearing fields. The isolate was subjected to morphological identification followed by DNA barcoding analysis by sequencing the ITS1-5.8S-ITS2 region. The isolate was identified morphologically up to genus level. For molecular identification, the DNA was first isolated, amplified by using PCR followed by sequencing the ITS region. A phylogenetic tree was also constructed based on the data obtained from sequencing to trace the evolutionary history of the isolated fungus. The fungal species was identified as Beauveria bassiana with 98.6% sequence similarity with already documented B. bassiana strains. The toxicity of the isolated fungus was also evaluated against A. assamensis larvae. The LC50 value was determined at 96 hr and recorded at 1.1x108 spore/ ml concentration.
本研究试图从饲养田中采集的受白僵菌感染的 Antheraea assamensis Helfer(木蚕)幼虫中分离并鉴定 Beauveria sp.。先对分离物进行形态鉴定,然后通过 ITS1-5.8S-ITS2 区域测序进行 DNA 条形码分析。分离物的形态鉴定达到了属的水平。为了进行分子鉴定,首先分离出 DNA,利用 PCR 进行扩增,然后对 ITS 区域进行测序。还根据测序获得的数据构建了系统发生树,以追溯分离真菌的进化史。经鉴定,该真菌为 Beauveria bassiana,与已有文献记载的 B. bassiana 菌株序列相似度为 98.6%。还评估了分离出的真菌对 A. assamensis 幼虫的毒性。在 1.1x108 个孢子/毫升的浓度下,96 小时后测定了 LC50 值。
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引用次数: 0
Efficacy of Ready-Mix Insecticides against Fall Army Worm Spodoptera frugiperda (J E Smith) on Maize 即用型混配杀虫剂对玉米上的秋军虫 Spodoptera frugiperda (J E Smith) 的药效
Pub Date : 2024-02-01 DOI: 10.55446/ije.2024.1453
Patil S. A., Kadam D. R., Deshmukh K. V., Bankar D. R., Parjane N. V.
During kharif 2019, the efficacy of combined insecticides against the fall army worm Spodoptera frugiperda (J E Smith) in maize was assessed. Chlorantraniliprole 9.3%+ lambdacyhalothrin 4.6%ZC was the most effective. Fipronil 4% + acetamiprid 4%SC was less effective. All treatments were effective in increasing the yield- chlorantraniliprole 9.3% + lambdacyhalothrin 4.6 %ZC gave maximum grain yield (40.70 q/ ha) while maximum benefit cost ratio was obtained with thiamethoxam 12.6 + lambdacyhalothrin 9.5%ZC (1:28.33).
在 2019 年的种植季节,评估了联合杀虫剂对玉米上的落叶军虫 Spodoptera frugiperda (J E Smith) 的防效。氯虫苯甲酰胺 9.3%+高效氯氟氰菊酯 4.6%ZC最有效。氟虫腈 4%+啶虫脒 4%ZC 的效果较差。所有处理都能有效提高产量--氯氰菊酯 9.3%+ 溴氰菊酯 4.6%ZC 的处理可获得最高的谷物产量(40.70 q/ ha),而噻虫嗪 12.6+ 溴氰菊酯 9.5%ZC 的处理可获得最高的效益成本比(1:28.33)。
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引用次数: 0
Effect of Newer Insecticides against the Major Insect Pests of Groundnut 新型杀虫剂对花生主要害虫的影响
Pub Date : 2024-02-01 DOI: 10.55446/ije.2024.1868
N. Priyanka, O. Shaila, M. Anuradha, V. Divya Rani, M. Rajashekhar
Efficacy of some newer insecticides were tested against the groundnut leaf miner Aproaerema modicella (Deventer), tobacco caterpillar Spodoptera litura (F), gram pod borer Helicoverpa armigera (Hubner) and natural enemies (coccinellids) of groundnut. Tolfenpyrad @ 2.5ml/ l was significantly superior in controlling defoliator pests. The next best treatments were tolfenpyrad @ 2ml/ l, spinetoram @ 0.5ml/l, tolfenpyrad @ 1.5ml/ l, thiamethoxam+ lambdacyhalothrin @ 0.4ml/ l. The highest incremental cost benefit ratio (ICBR) was obtained in the treatments with tolfenpyrad @ 2.5ml/ l.
测试了一些新型杀虫剂对花生潜叶蛾 Aproaerema modicella (Deventer)、烟草毛虫 Spodoptera litura (F)、禾谷荚螟 Helicoverpa armigera (Hubner) 和花生天敌(coccinellids)的药效。唑虫酰胺(2.5 毫升/升)在防治落叶害虫方面效果显著。其次是2毫升/升的噻虫嗪、0.5毫升/升的辛硫磷、1.5毫升/升的噻虫嗪、0.4毫升/升的噻虫胺+溴氰菊酯。
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引用次数: 0
Characterization of Dominant Cuticular Hydrocarbons in Inversion and Inversion-Free Strains of Drosophila ananassae (Doleschall) 反转和无反转果蝇品系中主要角质层碳氢化合物的特征
Pub Date : 2024-02-01 DOI: 10.55446/ije.2024.1275
K. R S, Jayaramu S. C.
Cuticular hydrocarbons (CHCs) of Drosophila ananassae (Doleschall) was characterized and identified using gas chromatography and mass spectrometry (GC-MS) analysis. A high % of methyl-branched alkanes were identified in all inversion (2LA, 3LA and 2LA+3LA) and inversion-free strains followed by linear alkanes and alkenes. The present work unfolds the significant patterns of variations in the isomeric forms of methylated alkanes between the inversion and inversion free strains (F-46.6; df-3, p 0.005), and non-significant between the sex (F-2.14; df-1, p 0.2394). But in linear alkanes shows significant variation between the inversion strains (F-30.49; df-3, p 0.009) and between the male and female (F-115.45; df-1, p 0.001) was observed. In particular there is a significant correlation between the chromosomal inversion and synthesis of CHCs in D. ananassae. Unique blend of CHCs in Drosophila performs dual role as desiccation resistance and act as chemical signalling molecule. Linear alkanes are majorly involved in desiccation resistance but in methyl- branched CHCs length variation is a key determinant of desiccation resistance. Presence of longer methyl- branched alkanes and higher desiccation resistance, shorter the carbon chain length act as a signalling molecules. The current study revealed the influence of chromosomal inversion on the cuticular hydrocarbon profile in D. ananassae.
利用气相色谱和质谱(GC-MS)分析方法对 ananassae(Doleschall)果蝇的角质碳氢化合物(CHCs)进行了表征和鉴定。在所有反转菌株(2LA、3LA 和 2LA+3LA)和无反转菌株中发现了高比例的甲基支链烷烃,其次是线性烷烃和烯烃。本研究揭示了反转菌株和无反转菌株之间甲基化烷烃异构体的显著变化规律(F-46.6;df-3,p 0.005),而性别之间的差异不显著(F-2.14;df-1,p 0.2394)。但在线性烷烃中,观察到反转品系之间(F-30.49;df-3,p 0.009)和雌雄之间(F-115.45;df-1,p 0.001)有显著差异。特别是,D. ananassae 的染色体反转与 CHCs 合成之间存在明显的相关性。果蝇中独特的 CHCs 混合体具有抗干燥和作为化学信号分子的双重作用。线性烷烃主要参与抗干燥性,但甲基支链 CHC 的长度变化是决定抗干燥性的关键因素。甲基支链烷烃的长度越长,抗干燥性越高,碳链长度越短,其信号分子的作用就越大。目前的研究揭示了染色体反转对 D. ananassae 角质层碳氢化合物特征的影响。
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引用次数: 0
Bioecology of Reduviid Rhynocoris Rapax- Predator of Podagrica decolorata 红腹角雉(Rhynocoris Rapax)的生物生态学- Podagrica decolorata 的天敌
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.1596
O. Adagba, Yao N’guessan, Tano Djè Kévin Christian, Ossey Christian Landry, Soro Senan
Flea beetle Podagrica decolorata (Duvivier) is a defoliator of okra. The study of some biological parameters of bedbug, Rhynocoris rapax (Stal) was carried out in locality of Man, The study is part of the control of this crop pest. Boxes were used to rear the insects. A thermohygrometer was used to determine the regular temperature and humidity. Larval monitoring revealed five larval stages, with the longest development time of 16.28±2.41 days for stage 5. The highest survival rate was observed for fifth-stage larvae (96.09± 03.14%). Female biological cycle was an mean 82.10± 09.41 days, and male biological cycle was an mean 68.67± 08.44 days. The sex ratio was 0.58. This study revealed positive correlations between humidity and the larval cycle, followed by negative correlations between temperature and the larval cycle.
跳甲 Podagrica decolorata (Duvivier) 是秋葵的一种落叶害虫。在马恩地区对臭虫 Rhynocoris rapax (Stal) 的一些生物参数进行了研究,该研究是控制这种农作物害虫的一部分。使用盒子饲养昆虫。使用温湿度计测定常规温度和湿度。幼虫监测显示有五个幼虫阶段,第五阶段的发育时间最长,为 16.28±2.41 天。第五期幼虫的存活率最高(96.09± 03.14%)。雌性生物周期平均为(82.10± 09.41)天,雄性生物周期平均为(68.67± 08.44)天。性别比为 0.58。该研究表明,湿度与幼虫周期呈正相关,温度与幼虫周期呈负相关。
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引用次数: 0
Partial Solar Eclipse Affects Apis Mellifera L Forging Activity 日偏食影响蜜蜂的锻造活动
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.708
Amit Choudhary, Bharathi Mohindru, Ramandeep Kaur, Jaspal Singh, P. Chhuneja
Bees use sun’s position for navigation and thus a solar eclipse may affect this capability. Present study was conducted during partial solar eclipse on June 21, 2020 at Ludhiana, Punjab. Apis mellifera L foragers’ activity at the hive entrance was recorded through a digital bee counter. Bees’ activity increased normally, i.e. 771 and 823 bees/ 15 mins coming in and going out of the hive, respectively before the start of eclipsephase. At peak obscuration it decreased slightly but non-significantly. Changes in hive temperature andhumidity might have limited the recruitment of foragers. Hence, no peak activity was recorded duringpeak obscuration, which otherwise was recorded on ordinary days at 1345 hr.
蜜蜂利用太阳的位置进行导航,因此日食可能会影响其导航能力。本研究于 2020 年 6 月 21 日日偏食期间在旁遮普邦卢迪亚纳进行。蜜蜂计数器记录了蜜蜂在蜂巢入口处的活动。在日食开始前,蜜蜂活动量正常增加,即每 15 分钟分别有 771 和 823 只蜜蜂进出蜂巢。在日食高峰期,蜜蜂活动量略有下降,但降幅不大。蜂巢温度和湿度的变化可能限制了觅蜂活动。因此,在日食高峰期没有记录到活动高峰,而在平时的 1345 小时则记录到活动高峰。
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引用次数: 0
Diversity of Pollinators on Sunflower 向日葵上传粉昆虫的多样性
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.1631
D. Padhy, C. R. Satapathy, S. Borkataki
Sunflower crop was grown during rabi 2021 and summer 2022 at the M S Swaminathan School of Agriculture, Centurion University of Technology and Management, Gajapati, Odisha. Investigation has been made on diversity of different pollinating agents associated with sunflower by observing their abundance, diversity, percentage contribution following the statistical diversity indices. The present study revealed eighteen different pollinators associated with sunflower. The Indian honey bee, Apis cerana indica (F)was recorded as the most dominant hymenopteran pollinator followed by the rock bee Apis dorsata(F)from family Apidae. The foraging activity of A. c. indica was observed to be maximum during midflowering stage (1.72± 1.23 and 2.78± 1.81 bees/ capitulum/ min in 1st and 2nd season, respectively) withhighest activity during 01:00-02:00 PM (3.02 bees/ capitulum/min.) and 10:00 to 11:00 AM (4.42 bees/capitulum/ min) during first and second seasons respectively. The Apidae family members’ i.e. A. c. indica and A. dorsata have attained eudominant and other pollinators were belonging mostly to the recedentand subrecedent classes based on dominance status. The diversity indices showed mid flowering stageattracted the most diversified group of pollinators.
向日葵作物于 2021 年雨季和 2022 年夏季在奥迪沙邦加贾帕蒂的 Centurion 技术与管理大学 M S Swaminathan 农学院种植。通过观察向日葵不同授粉媒介的丰度、多样性、贡献百分比以及统计多样性指数,对向日葵不同授粉媒介的多样性进行了调查。本研究揭示了与向日葵相关的 18 种不同授粉媒介。据记录,印度蜜蜂(Apis cerana indica,F)是最主要的膜翅目传粉媒介,其次是喙蜂科的岩蜂(Apis dorsata,F)。在第一季和第二季中,中蜂的觅食活动在开花中期最为活跃(第一季和第二季分别为 1.72± 1.23 和 2.78± 1.81 只蜜蜂/头状花序/分钟),在下午 01:00-02:00 (3.02 只蜜蜂/头状花序/分钟)和上午 10:00-11:00 (4.42 只蜜蜂/头状花序/分钟)时最为活跃。根据优势地位,蛛形纲成员(A. c. indica 和 A. dorsata)已达到优势地位,其他传粉昆虫大多属于后进和次后进级别。多样性指数显示,花期中期吸引了最多样化的传粉昆虫。
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引用次数: 0
Effect of Spermidine Supplementation on Testicular And Ovarian Development in Sex-limited and Non-sex-limited Bivoltine Silk Worm Breeds of Bombyx mori L 补充精脒对有性限和无性限双孢菇蚕种睾丸和卵巢发育的影响
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.1585
Madhavi Kasa, Brinda Goda Lakshmi Didugu, Seetharamulu Jolapuram, Jagannatha Raju Poosapati, Anitha Mamillapalli
Silk worm Bombyx mori L is a holometabolous, lepidopteran model for investigating the effect of various molecules. The sex-limited character of cocoon color is useful for the separation of male and female pupae during commercial seed production. Fecundity is the major limitation of cocoon colour sex-limited breeds. Spermidine is a polyamine, present in all living cells involved in the growth and reproduction of many organisms. The effect of spermidine on testicular and ovarian development in the selected bivoltine sex-limited breed; APS27SL and non-sex-limited hybrid; APS45 x APS12 is studied in the present work. Results showed that foliar feeding of spermidine significantly increased pupal growth, testicular and ovarian growth, number of ovarioles and ovules in the sex-limited breed. Moreover, increased expression of Bm-tektin was observed in the testes of spermidine fed groups. Thus, the study suggests that foliar feeding of spermidine can be used to overcome the limitation of reproductive potential in sex-limited breeds.
丝虫 Bombyx mori L 是一种全代谢鳞翅目昆虫模型,可用于研究各种分子的影响。茧色的性别限制特性有助于在商业种子生产过程中分离雌雄蛹。繁殖力是茧色性别限制品种的主要限制因素。精胺是一种多胺,存在于所有生物细胞中,参与许多生物的生长和繁殖。本实验研究了精胺对所选双峰性限制品种 APS27SL 和非性限制杂交种 APS45 x APS12 的睾丸和卵巢发育的影响。结果表明,叶面喷施亚精胺能显著提高有性限品种的蛹的生长、睾丸和卵巢的生长、卵巢和胚珠的数量。此外,在喂食亚精胺组的睾丸中观察到 Bm-tektin 的表达增加。因此,该研究表明,叶面喷施亚精胺可用于克服性别受限品种的繁殖潜力限制。
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引用次数: 0
Overview of Predators in Shallots Plantation in Peatland, Landasan Ulin South Borneo 南婆罗洲兰达山乌林泥炭地大葱种植园掠食者概况
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.1598
M. Pramudi, Samharinto, H. O. Rosa, Salamiah, L. Aphrodyanti
Species abundance and diversity of predatory arthropods are important information for developing biological pest control strategies. A study that aims to determine the abundance of predators (Coccinellidae and Araneae) in peatlands planted with shallots in Tegal Arum Village, Landasan Ulin District, Banjarbaru City, South Borneo has been carried out. The method used is purposive sampling method. Selecting plots with uniform growth and plant height of shallots in one plot or beds measuring 3 x 20 m and six plots were observed using three methods: direct sampling, swing nets, and pitfall traps. Observations on species abundance of spiders and predatory coccinellids were carried out to describe the number of species and the abundance of insects in shallot cultivation on peatlands. Predators from the order Coleoptera of the Coccinellidae family observed belong to (Menochilus sexmaculatus, Micrapsis sp, Coccinella novemnotata and Scymnus sp. with 143 individuals and the highest population was found by Micrapsis sp (128 individuals). Meanwhile, there were 6 species of spiders (Araneae) from 5 different families (Oxyopidae, Tetragnatidae, Lyniphiidae, Thomisidae, and Lycosidae) with the most individuals being Oxyopes variabilis (Araneidae: Oxyopidae). The distribution index of species diversity (E) shows E> 0.6, indicating that the evenness of species on the land is uneven
捕食性节肢动物的物种丰度和多样性是制定生物害虫控制策略的重要信息。本研究旨在确定南婆罗洲班贾巴鲁市兰达山乌林区泰加尔阿鲁姆村种植有香葱的泥炭地中捕食性节肢动物(Coccinellidae和Araneae)的数量。采用的方法是目的取样法。在一个面积为 3 x 20 米的地块或花圃中选择生长和植株高度一致的香葱地块,使用直接取样、摇摆网和坑式陷阱三种方法对六个地块进行了观察。对蜘蛛和捕食性茧翅目昆虫的物种丰度进行了观察,以描述泥炭地大葱种植中昆虫的物种数量和丰度。观察到的鞘翅目茧丝虫科捕食茧丝虫属(Menochilus sexmaculatus、Micrapsis sp、Coccinella novemnotata和Scymnus sp.)有143只,其中Micrapsis sp种群数量最多(128只)。同时,有来自 5 个不同科(Oxyopidae、Tetragnatidae、Lyniphiidae、Thomisidae 和 Lycosidae)的 6 种蜘蛛(Araneae),其中个体数最多的是 Oxyopes variabilis(Araneidae:Oxyopidae)。物种多样性分布指数(E)显示 E> 0.6,表明陆地上物种分布不均匀
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引用次数: 0
New Records of the Genus Tachys Casey (Coleoptera: Carabidae: Trechinae) from India 来自印度的 Tachys Casey 属新记录(鞘翅目:食肉目:棘皮目
Pub Date : 2024-01-23 DOI: 10.55446/ije.2024.1713
Shigina. K, Sabu K. Thomas
New records two Carabidae species [Tachys plagiatus Putzeys, 1875 and Tachys tropicus Nietner, 1858 (Coleoptera: Carabidae: Trechinae)] from India with detailed descriptions and notes on their geographical distribution are provided.
本报告提供了来自印度的两个鞘翅目物种 [Tachys plagiatus Putzeys, 1875 和 Tachys tropicus Nietner, 1858 (Coleoptera: Carabidae: Trechinae)] 的新记录及其详细描述和地理分布说明。
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引用次数: 0
期刊
Indian journal of entomology
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