Objectives: Oxidative stress (OS) and oxidative DNA damage accruing from chronic exposure to wood dust have been implicated in the development of chronic lung conditions among woodworkers. Indices of OS, inflammation, oxidative DNA damage and lung function in relation to duration of exposure to wood dust were assessed in woodworkers to determine their possible utility as risk evaluation indices for chronic lung conditions.
Methods: Ninety participants comprising 30 active woodworkers, 30 passive woodworkers, and 30 controls were enrolled into this cross-sectional study. The total plasma peroxides, total antioxidant capacity (TAC), oxidative stress index (OSI), malondialdehyde (MDA), reduced glutathione, nitric oxide, high sensitivity C-reactive protein (hs-CRP), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and peak expiratory flow rate (PEFR) were determined in all participants.
Results: Woodworkers had lower PEFR, TAC, and higher malondialdehyde, OSI, hs-CRP, and 8-OHdG compared to controls (p < 0.05). Active woodworkers had higher malondialdehyde, 8-OHdG, and hs-CRP compared to passive woodworkers (p < 0.05). Increasing duration of exposure to wood dust is associated with higher malondialdehyde, hs-CRP, and 8-OHdG in active woodworkers (p < 0.05) and higher 8-OHdG and hs-CRP in passive woodworkers (p < 0.05). Negative correlation was observed between hs-CRP and TAC (r=-0.367, p = 0.048) in active workers.
Conclusion: The association of exposure to wood dust with elevated indices of inflammation, OS, lipid peroxidation, oxidative DNA damage, and reduction in antioxidants and peak expiratory flow rate; and the concomitant increase in oxidative DNA damage and inflammation with increasing duration of exposure suggest that these indices may be useful in predicting woodworkers at risk of development of chronic lung conditions.
目的：氧化应激（OS）和长期暴露于木尘引起的DNA氧化损伤与木工慢性肺部疾病的发展有关。在木工中评估了OS、炎症、氧化DNA损伤和肺功能指标与暴露于木尘的持续时间的关系，以确定它们作为慢性肺部疾病风险评估指标的可能用途。方法：90名参与者，包括30名主动木工、30名被动木工和30名对照，被纳入这项横断面研究。测定所有参与者的总血浆过氧化物、总抗氧化能力（TAC）、氧化应激指数（OSI）、丙二醛（MDA）、还原型谷胱甘肽、一氧化氮、高敏C反应蛋白（hs-CRP）、8-羟基-2'-脱氧鸟苷（8-OHdG）和呼气峰流速（PEFR）。结果：与对照组相比，木工的PEFR、TAC较低，丙二醛、OSI、hs-CRP和8-OHdG较高（p p p p r=-0.367，p = 0.048）。结论：木尘暴露与炎症、OS、脂质过氧化、DNA氧化损伤指数升高、抗氧化剂和呼气峰流速降低有关；随着暴露时间的增加，氧化性DNA损伤和炎症也随之增加，这表明这些指标可能有助于预测木工患慢性肺部疾病的风险。
{"title":"Perturbations in indices of oxidative stress, oxidative DNA damage and lung function in chronic exposure to wood dust in Southern Nigeria.","authors":"Augusta Chinyere Nsonwu-Anyanwu, Raymond Ekong Eworo, Unyime Aniekpon Fabian, Uduak Luke, Caroline Chinenyenwa Thomas, Olivia Kamsi Muoka, Unwanaabasi Okon Ufot, Chinyere Adanna Opara Usoro","doi":"10.1080/08958378.2023.2224388","DOIUrl":"10.1080/08958378.2023.2224388","url":null,"abstract":"<p><strong>Objectives: </strong>Oxidative stress (OS) and oxidative DNA damage accruing from chronic exposure to wood dust have been implicated in the development of chronic lung conditions among woodworkers. Indices of OS, inflammation, oxidative DNA damage and lung function in relation to duration of exposure to wood dust were assessed in woodworkers to determine their possible utility as risk evaluation indices for chronic lung conditions.</p><p><strong>Methods: </strong>Ninety participants comprising 30 active woodworkers, 30 passive woodworkers, and 30 controls were enrolled into this cross-sectional study. The total plasma peroxides, total antioxidant capacity (TAC), oxidative stress index (OSI), malondialdehyde (MDA), reduced glutathione, nitric oxide, high sensitivity C-reactive protein (hs-CRP), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and peak expiratory flow rate (PEFR) were determined in all participants.</p><p><strong>Results: </strong>Woodworkers had lower PEFR, TAC, and higher malondialdehyde, OSI, hs-CRP, and 8-OHdG compared to controls (<i>p</i> < 0.05). Active woodworkers had higher malondialdehyde, 8-OHdG, and hs-CRP compared to passive woodworkers (<i>p</i> < 0.05). Increasing duration of exposure to wood dust is associated with higher malondialdehyde, hs-CRP, and 8-OHdG in active woodworkers (<i>p</i> < 0.05) and higher 8-OHdG and hs-CRP in passive woodworkers (<i>p</i> < 0.05). Negative correlation was observed between hs-CRP and TAC (<i>r</i>=-0.367, <i>p</i> = 0.048) in active workers.</p><p><strong>Conclusion: </strong>The association of exposure to wood dust with elevated indices of inflammation, OS, lipid peroxidation, oxidative DNA damage, and reduction in antioxidants and peak expiratory flow rate; and the concomitant increase in oxidative DNA damage and inflammation with increasing duration of exposure suggest that these indices may be useful in predicting woodworkers at risk of development of chronic lung conditions.</p>","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9631305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1080/08958378.2022.2140228
María-de-Los-Angeles Andrade-Oliva, Yazmín Debray-García, Guadalupe-Elide Morales-Figueroa, Juan Escamilla-Sánchez, Omar Amador-Muñoz, Raúl V Díaz-Godoy, Michael Kleinman, Benjamín Florán, José-Antonio Arias-Montaño, Andrea De Vizcaya-Ruiz
Alterations in dopaminergic transmission are associated with neurological disorders, such as depression, autism, and Parkinson's disease. Exposure of rats to ambient fine (FP) or ultrafine (UFP) particles induces oxidative and inflammatory responses in the striatum, a neuronal nucleus with dense dopaminergic innervation and critically involved in the control of motor activity.Objectives: We used an ex vivo system to evaluate the effect of in vivo inhalation exposure to FP and UFP on motor activity and dopaminergic transmission.Materials and Methods: Male adult Wistar rats were exposed to FP, UFP, or filtered air for 8 weeks (subchronic exposure; 5 h/day, 5 days/week) in a particle concentrator. Motor activity was evaluated using the open-field test. Uptake and release of [3H]-dopamine were assessed in striatal synaptosomes, and dopamine D2 receptor (D2R) affinity for dopamine was evaluated by the displacement of [3H]-spiperone binding to striatal membranes.Results: Exposure to FP or UFP significantly reduced spontaneous motor activity (ambulatory distance: FP -25%, UFP -32%; ambulatory time: FP -24%, UFP -22%; ambulatory episodes: FP -22%, UFP -30%), decreased [3H]-dopamine uptake (FP -18%, UFP -24%), and increased, although not significantly, [3H]-dopamine release (113.3 ± 16.3 and 138.6 ± 17.3%). Neither FP nor UFP exposure affected D2R density or affinity for dopamine.Conclusions: These results indicate that exposure to ambient particulate matter reduces locomotion in rats, which could be related to altered striatal dopaminergic transmission: UFP was more potent than FP. Our results contribute to the evidence linking environmental factors to changes in brain function that could turn into neurological and psychiatric disorders.HIGHLIGHTSYoung adult rats were exposed to fine (FP) or ultrafine (UFP) particles for 40 days.Exposure to FP or UFP reduced motor activity.Exposure to FP or UFP reduced dopamine uptake by striatal synaptosomes.Neither D2R density or affinity for dopamine was affected by FP or UFP.UFP was more potent than FP to exert the effects reported.
{"title":"Effect of subchronic exposure to ambient fine and ultrafine particles on rat motor activity and <i>ex vivo</i> striatal dopaminergic transmission.","authors":"María-de-Los-Angeles Andrade-Oliva, Yazmín Debray-García, Guadalupe-Elide Morales-Figueroa, Juan Escamilla-Sánchez, Omar Amador-Muñoz, Raúl V Díaz-Godoy, Michael Kleinman, Benjamín Florán, José-Antonio Arias-Montaño, Andrea De Vizcaya-Ruiz","doi":"10.1080/08958378.2022.2140228","DOIUrl":"https://doi.org/10.1080/08958378.2022.2140228","url":null,"abstract":"<p><p>Alterations in dopaminergic transmission are associated with neurological disorders, such as depression, autism, and Parkinson's disease. Exposure of rats to ambient fine (FP) or ultrafine (UFP) particles induces oxidative and inflammatory responses in the striatum, a neuronal nucleus with dense dopaminergic innervation and critically involved in the control of motor activity.<b>Objectives:</b> We used an <i>ex vivo</i> system to evaluate the effect of <i>in vivo</i> inhalation exposure to FP and UFP on motor activity and dopaminergic transmission.<b>Materials and Methods:</b> Male adult Wistar rats were exposed to FP, UFP, or filtered air for 8 weeks (subchronic exposure; 5 h/day, 5 days/week) in a particle concentrator. Motor activity was evaluated using the open-field test. Uptake and release of [<sup>3</sup>H]-dopamine were assessed in striatal synaptosomes, and dopamine D<sub>2</sub> receptor (D<sub>2</sub>R) affinity for dopamine was evaluated by the displacement of [<sup>3</sup>H]-spiperone binding to striatal membranes.<b>Results:</b> Exposure to FP or UFP significantly reduced spontaneous motor activity (ambulatory distance: FP -25%, UFP -32%; ambulatory time: FP -24%, UFP -22%; ambulatory episodes: FP -22%, UFP -30%), decreased [<sup>3</sup>H]-dopamine uptake (FP -18%, UFP -24%), and increased, although not significantly, [<sup>3</sup>H]-dopamine release (113.3 ± 16.3 and 138.6 ± 17.3%). Neither FP nor UFP exposure affected D<sub>2</sub>R density or affinity for dopamine.<b>Conclusions:</b> These results indicate that exposure to ambient particulate matter reduces locomotion in rats, which could be related to altered striatal dopaminergic transmission: UFP was more potent than FP. Our results contribute to the evidence linking environmental factors to changes in brain function that could turn into neurological and psychiatric disorders.HIGHLIGHTSYoung adult rats were exposed to fine (FP) or ultrafine (UFP) particles for 40 days.Exposure to FP or UFP reduced motor activity.Exposure to FP or UFP reduced dopamine uptake by striatal synaptosomes.Neither D<sub>2</sub>R density or affinity for dopamine was affected by FP or UFP.UFP was more potent than FP to exert the effects reported.</p>","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9295248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1080/08958378.2022.2149905
Sara Pouriamehr, Valiollah Dabidi Roshan, Farimah Shirani
Purpose: As consequences of industrial processes, air pollution has led to increased cardiovascular diseases resulting in mortality. However, there are few pieces of evidence expressing physical fitness and gender impacts in such environments. Regarding long-term exposure to air pollution, this study aimed to determine the effect of physical fitness on post-exercise cardiac parasympathetic reactivation among healthy males and females.
Methods: 120 individuals (46 ± 5 years) participated and were categorized into two main groups (n = 60; EG, CG); (1) The experimental group included individuals living in an air-polluted environment; (2) The control group included the citizens of a clean air region; and two physical fitness status subgroups (n = 30; active vs. sedentary) across both sexes. The heart rate (HR) changes at different timing after performing an incremental exercise, and T30 were calculated as metrics of cardiac parasympathetic reactivation.
Results: The heart rate recovery values were substantially lower in EG in comparison to CG (p < 0.001) at different timing, while, T30 was significantly greater in residents of the air-polluted city compared to CG (p < 0.001). As for heart rate recovery at the 5th minute, the values were significantly lower in the steady-female group in comparison to the active females living in the air-polluted city (p < 0.01).
Conclusion: Based on our findings, although physical fitness modifies the adverse impacts of long-term exposure to air pollution on post-exercise cardio-parasympathetic reactivation, it appears to parallel the acute/intermediate recovery of the thermoregulatory and vascular systems, among both sexes, it does not prevent them.
{"title":"Does long-term exposure to air pollution suppress parasympathetic reactivation after incremental exercise among healthy males and females?","authors":"Sara Pouriamehr, Valiollah Dabidi Roshan, Farimah Shirani","doi":"10.1080/08958378.2022.2149905","DOIUrl":"https://doi.org/10.1080/08958378.2022.2149905","url":null,"abstract":"<p><strong>Purpose: </strong>As consequences of industrial processes, air pollution has led to increased cardiovascular diseases resulting in mortality. However, there are few pieces of evidence expressing physical fitness and gender impacts in such environments. Regarding long-term exposure to air pollution, this study aimed to determine the effect of physical fitness on post-exercise cardiac parasympathetic reactivation among healthy males and females.</p><p><strong>Methods: </strong>120 individuals (46 ± 5 years) participated and were categorized into two main groups (<i>n</i> = 60; EG, CG); (1) The experimental group included individuals living in an air-polluted environment; (2) The control group included the citizens of a clean air region; and two physical fitness status subgroups (<i>n</i> = 30; active vs. sedentary) across both sexes. The heart rate (HR) changes at different timing after performing an incremental exercise, and T30 were calculated as metrics of cardiac parasympathetic reactivation.</p><p><strong>Results: </strong>The heart rate recovery values were substantially lower in EG in comparison to CG (<i>p</i> < 0.001) at different timing, while, T30 was significantly greater in residents of the air-polluted city compared to CG (<i>p</i> < 0.001). As for heart rate recovery at the 5th minute, the values were significantly lower in the steady-female group in comparison to the active females living in the air-polluted city (<i>p</i> < 0.01).</p><p><strong>Conclusion: </strong>Based on our findings, although physical fitness modifies the adverse impacts of long-term exposure to air pollution on post-exercise cardio-parasympathetic reactivation, it appears to parallel the acute/intermediate recovery of the thermoregulatory and vascular systems, among both sexes, it does not prevent them.</p>","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10726066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The air quality index (AQI) forecasts are one of the most important aspects of improving urban public health and enabling society to remain sustainable despite the effects of air pollution. Pollution control organizations deploy ground stations to collect information about air pollutants. Establishing a ground station all-around is not feasible due to the cost involved. As an alternative, satellite-captured data can be utilized for AQI assessment. This study explores the changes in AQI during various COVID-19 lockdowns in India utilizing satellite data. Furthermore, it addresses the effectiveness of state-of-the-art deep learning and statistical approaches for forecasting short-term AQI.
Materials and methods: Google Earth Engine (GEE) has been utilized to capture the data for the study. The satellite data has been authenticated against ground station data utilizing the beta distribution test before being incorporated into the study. The AQI forecasting has been explored using state-of-the-art statistical and deep learning approaches like VAR, Holt-Winter, and LSTM variants (stacked, bi-directional, and vanilla).
Results: AQI ranged from 100 to 300, from moderately polluted to very poor during the study period. The maximum reduction was recorded during the complete lockdown period in the year 2020. Short-term AQI forecasting with Holt-Winter was more accurate than other models with the lowest MAPE scores.
Conclusions: Based on our findings, air pollution is clearly a threat in the studied locations, and it is important for all stakeholders to work together to reduce it. The level of air pollutants dropped substantially during the different lockdowns.
{"title":"Analysis and forecasting of air quality index based on satellite data.","authors":"Tinku Singh, Nikhil Sharma, Satakshi, Manish Kumar","doi":"10.1080/08958378.2022.2164388","DOIUrl":"https://doi.org/10.1080/08958378.2022.2164388","url":null,"abstract":"<p><strong>Objective: </strong>The air quality index (AQI) forecasts are one of the most important aspects of improving urban public health and enabling society to remain sustainable despite the effects of air pollution. Pollution control organizations deploy ground stations to collect information about air pollutants. Establishing a ground station all-around is not feasible due to the cost involved. As an alternative, satellite-captured data can be utilized for AQI assessment. This study explores the changes in AQI during various COVID-19 lockdowns in India utilizing satellite data. Furthermore, it addresses the effectiveness of state-of-the-art deep learning and statistical approaches for forecasting short-term AQI.</p><p><strong>Materials and methods: </strong>Google Earth Engine (GEE) has been utilized to capture the data for the study. The satellite data has been authenticated against ground station data utilizing the beta distribution test before being incorporated into the study. The AQI forecasting has been explored using state-of-the-art statistical and deep learning approaches like VAR, Holt-Winter, and LSTM variants (stacked, bi-directional, and vanilla).</p><p><strong>Results: </strong>AQI ranged from 100 to 300, from moderately polluted to very poor during the study period. The maximum reduction was recorded during the complete lockdown period in the year 2020. Short-term AQI forecasting with Holt-Winter was more accurate than other models with the lowest MAPE scores.</p><p><strong>Conclusions: </strong>Based on our findings, air pollution is clearly a threat in the studied locations, and it is important for all stakeholders to work together to reduce it. The level of air pollutants dropped substantially during the different lockdowns.</p>","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9296275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-13DOI: 10.1080/08958378.2022.2086650
K. Sadakane, T. Ichinose, T. Maki, M. Nishikawa
Abstract Aims Asian sand dust (ASD) comprises soil particles, microorganisms, and various chemical components. We examined whether peptidoglycan (PGN), a structural cell wall component of Gram-positive bacteria, exacerbates ASD-induced allergic airway inflammation in mice. Methods The ASD (median diameter ∼4 µm) used was a certified reference material from the National Institute for Environmental Studies in Japan, derived from Gobi Desert surface soil collected in 2011. BALB/c mice were intratracheally exposed to PGN, heat-inactivated ASD (H-ASD), and ovalbumin (OVA), individually and in combination. Twenty-four hours after the final intratracheal administration, bronchoalveolar lavage fluid (BALF) and serum samples were collected. Inflammatory cell count, cytokine levels in the BALF, OVA-specific immunoglobulin levels in the serum, and pathological changes in the lungs were analyzed. Results and Discussion After OVA + PGN + H-ASD treatment, the number of eosinophils, neutrophils, and macrophages in the BALF and of eosinophils in the lung tissue was significantly higher than that after OVA + PGN or OVA + H-ASD treatment. Moreover, levels of chemokines and cytokines associated with eosinophil recruitment and activation were significantly higher in the BALF of this group than in that of the OVA + PGN group, and tended to be higher than those in the OVA + H-ASD group. Pathological changes in the lungs were most severe in mice treated with OVA + PGN + H-ASD. Conclusions Our results indicate that PGN is involved in the exacerbation of ASD-induced allergic airway inflammation in mice. Thus, inhalation of ASD containing Gram-positive bacteria may trigger allergic bronchial asthma.
{"title":"Co-exposure of peptidoglycan and heat-inactivated Asian sand dust exacerbates ovalbumin-induced allergic airway inflammation in mice","authors":"K. Sadakane, T. Ichinose, T. Maki, M. Nishikawa","doi":"10.1080/08958378.2022.2086650","DOIUrl":"https://doi.org/10.1080/08958378.2022.2086650","url":null,"abstract":"Abstract Aims Asian sand dust (ASD) comprises soil particles, microorganisms, and various chemical components. We examined whether peptidoglycan (PGN), a structural cell wall component of Gram-positive bacteria, exacerbates ASD-induced allergic airway inflammation in mice. Methods The ASD (median diameter ∼4 µm) used was a certified reference material from the National Institute for Environmental Studies in Japan, derived from Gobi Desert surface soil collected in 2011. BALB/c mice were intratracheally exposed to PGN, heat-inactivated ASD (H-ASD), and ovalbumin (OVA), individually and in combination. Twenty-four hours after the final intratracheal administration, bronchoalveolar lavage fluid (BALF) and serum samples were collected. Inflammatory cell count, cytokine levels in the BALF, OVA-specific immunoglobulin levels in the serum, and pathological changes in the lungs were analyzed. Results and Discussion After OVA + PGN + H-ASD treatment, the number of eosinophils, neutrophils, and macrophages in the BALF and of eosinophils in the lung tissue was significantly higher than that after OVA + PGN or OVA + H-ASD treatment. Moreover, levels of chemokines and cytokines associated with eosinophil recruitment and activation were significantly higher in the BALF of this group than in that of the OVA + PGN group, and tended to be higher than those in the OVA + H-ASD group. Pathological changes in the lungs were most severe in mice treated with OVA + PGN + H-ASD. Conclusions Our results indicate that PGN is involved in the exacerbation of ASD-induced allergic airway inflammation in mice. Thus, inhalation of ASD containing Gram-positive bacteria may trigger allergic bronchial asthma.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43858169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.1080/08958378.2022.2083275
Daojie Li, Shu-Wei Chen, Chun Liu, Baoxin Wei, Xiaoping Li
Abstract Aim: Ammonia is a toxic gas that not only causes environmental pollution, but also is harmful to human health after inhalation. Liver is an important detoxification organ that can convert external or metabolized toxic substances into nontoxic substances. However, the toxic effects of ammonia exposure on livers have not been well studied. Method: In this study, pigs were used as an animal model and were exposed to 80 ppm ammonia (8 h during 12 days), and then, RNA-seq were conducted to explore the key genes in response to high ammonia exposure in livers. Result: Gene set enrichment analysis (GSEA) showed that the genes associated with hypoxia, inflammatory response, and apoptosis were up-regulated in the ammonia group, but the genes associated with DNA replication, linoleic acid metabolism, and glycolysis were down-regulated. Totally, 556 differentially expressed genes (DEGs) including 54 genes that encode the transcription factors (TFs) were identified between the exposure and control groups. GO and KEGG pathway analysis suggested that these DEGs were involved in inflammatory response, oxidative stress, apoptosis, immune, and cell cycle. Furthermore, the TF-target interaction analysis showed that FOS, HIF-1α, JUNB, ATF3, REL, and KLF4 were important TFs in regulating the hepatic gene expression in response to high ammonia exposure. Conclusion: Altogether, our findings not only presented a comprehensive mRNA transcriptome profile of liver after high ammonia exposure, but also found some key genes and TFs that could be used to investigate the toxicity mechanism of high ammonia on livers.
{"title":"Liver transcriptome analysis reveals biological pathways and transcription factors in response to high ammonia exposure","authors":"Daojie Li, Shu-Wei Chen, Chun Liu, Baoxin Wei, Xiaoping Li","doi":"10.1080/08958378.2022.2083275","DOIUrl":"https://doi.org/10.1080/08958378.2022.2083275","url":null,"abstract":"Abstract Aim: Ammonia is a toxic gas that not only causes environmental pollution, but also is harmful to human health after inhalation. Liver is an important detoxification organ that can convert external or metabolized toxic substances into nontoxic substances. However, the toxic effects of ammonia exposure on livers have not been well studied. Method: In this study, pigs were used as an animal model and were exposed to 80 ppm ammonia (8 h during 12 days), and then, RNA-seq were conducted to explore the key genes in response to high ammonia exposure in livers. Result: Gene set enrichment analysis (GSEA) showed that the genes associated with hypoxia, inflammatory response, and apoptosis were up-regulated in the ammonia group, but the genes associated with DNA replication, linoleic acid metabolism, and glycolysis were down-regulated. Totally, 556 differentially expressed genes (DEGs) including 54 genes that encode the transcription factors (TFs) were identified between the exposure and control groups. GO and KEGG pathway analysis suggested that these DEGs were involved in inflammatory response, oxidative stress, apoptosis, immune, and cell cycle. Furthermore, the TF-target interaction analysis showed that FOS, HIF-1α, JUNB, ATF3, REL, and KLF4 were important TFs in regulating the hepatic gene expression in response to high ammonia exposure. Conclusion: Altogether, our findings not only presented a comprehensive mRNA transcriptome profile of liver after high ammonia exposure, but also found some key genes and TFs that could be used to investigate the toxicity mechanism of high ammonia on livers.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48787087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-18DOI: 10.1080/08958378.2022.2075493
Weixing Hao, K. F. Kapiamba, Varuni Abhayaratne, S. Usman, Yue-Wern Huang, Yang Wang
Abstract Introduction Secondhand smoke endangers both the environment and the health of nonsmokers. Due to the scarcity of repeatable data generated by human subjects, a system capable of generating representative secondhand smoke is essential for studying smoke properties. This work presents the design and validation of a filter-based system that could mimic the particle deposition and penetration in human respiratory system for secondhand smoke generation and characterization. Methods Guided by our study on characterizing size-dependent filtration efficiency of common materials, we identified three filter media that generate similar particle deposition efficiencies compared to different regions of the human respiratory system over a wide submicron size range. We demonstrated the performance of the proposed filter-based system using various operating conditions. Additionally, we compared the properties of secondhand smoke particles to those of primary smoke particles. Results The difference in aerosol deposition efficiencies between the filter-based system and the International Commission on Radiological Protection (ICRP) model was less than 10% in the size range of 30 to 500 nm. High concentrations of metals were detected in the secondhand smoke. The contents of Ni and Cr generated from the secondhand electronic cigarettes are at least 20 and 5 times above the regulated daily maximum intake amount. Conclusion Given the agreement in aerosol respiratory deposition between the filter-based system and the ICRP model, such a system can facilitate laboratory studies of secondhand smoke due to its simple structure, high repeatability, and ease of control while remaining free of human subjects.
{"title":"A filter-based system mimicking the particle deposition and penetration in human respiratory system for secondhand smoke generation and characterization","authors":"Weixing Hao, K. F. Kapiamba, Varuni Abhayaratne, S. Usman, Yue-Wern Huang, Yang Wang","doi":"10.1080/08958378.2022.2075493","DOIUrl":"https://doi.org/10.1080/08958378.2022.2075493","url":null,"abstract":"Abstract Introduction Secondhand smoke endangers both the environment and the health of nonsmokers. Due to the scarcity of repeatable data generated by human subjects, a system capable of generating representative secondhand smoke is essential for studying smoke properties. This work presents the design and validation of a filter-based system that could mimic the particle deposition and penetration in human respiratory system for secondhand smoke generation and characterization. Methods Guided by our study on characterizing size-dependent filtration efficiency of common materials, we identified three filter media that generate similar particle deposition efficiencies compared to different regions of the human respiratory system over a wide submicron size range. We demonstrated the performance of the proposed filter-based system using various operating conditions. Additionally, we compared the properties of secondhand smoke particles to those of primary smoke particles. Results The difference in aerosol deposition efficiencies between the filter-based system and the International Commission on Radiological Protection (ICRP) model was less than 10% in the size range of 30 to 500 nm. High concentrations of metals were detected in the secondhand smoke. The contents of Ni and Cr generated from the secondhand electronic cigarettes are at least 20 and 5 times above the regulated daily maximum intake amount. Conclusion Given the agreement in aerosol respiratory deposition between the filter-based system and the ICRP model, such a system can facilitate laboratory studies of secondhand smoke due to its simple structure, high repeatability, and ease of control while remaining free of human subjects.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47313779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-03DOI: 10.1080/08958378.2022.2070690
Jian Liu, Yong-Quan Dong, Xuejie Li, Weifang Wu
Abstract Background: Severe nitrogen oxide poisoning can lead to life-threatening pulmonary injury. Methods: we report two cases of severe nitrogen-oxide-induced hypoxia treated with veno-venous extracorporeal membrane oxygenation (ECMO). After exposure, the conditions of both patients continued to deteriorate despite maximal mechanical ventilation with a fraction of inspired oxygen of 100%; therefore, we started veno-venous ECMO. The times from presentation to the initiation of ECMO in the two patients were 1 and 2 days. The hypoxemia and respiratory failure improved quickly after ECMO support. Results: The patients were discharged without complications. The durations of ECMO for the two patients were 5 and 6 days. Conclusion: This report describes how early ECMO support was used to treat potentially fatal pulmonary injury after exposure to nitrogen oxide. The duration of the ECMO run is a critical determinant of patient survival.
{"title":"Successful treatment of pulmonary injury due to nitrogen oxide exposure with extracorporeal membrane oxygenation: a report of two cases and literature review","authors":"Jian Liu, Yong-Quan Dong, Xuejie Li, Weifang Wu","doi":"10.1080/08958378.2022.2070690","DOIUrl":"https://doi.org/10.1080/08958378.2022.2070690","url":null,"abstract":"Abstract Background: Severe nitrogen oxide poisoning can lead to life-threatening pulmonary injury. Methods: we report two cases of severe nitrogen-oxide-induced hypoxia treated with veno-venous extracorporeal membrane oxygenation (ECMO). After exposure, the conditions of both patients continued to deteriorate despite maximal mechanical ventilation with a fraction of inspired oxygen of 100%; therefore, we started veno-venous ECMO. The times from presentation to the initiation of ECMO in the two patients were 1 and 2 days. The hypoxemia and respiratory failure improved quickly after ECMO support. Results: The patients were discharged without complications. The durations of ECMO for the two patients were 5 and 6 days. Conclusion: This report describes how early ECMO support was used to treat potentially fatal pulmonary injury after exposure to nitrogen oxide. The duration of the ECMO run is a critical determinant of patient survival.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43256212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-27DOI: 10.1080/08958378.2022.2065388
L. Stockfelt, Yiyi Xu, A. Gudmundsson, J. Rissler, C. Isaxon, J. Brunskog, J. Pagels, P. Nilsson, M. Berglund, L. Barregard, M. Bohgard, M. Albin, I. Hagerman, A. Wierzbicka
Abstract Background Adverse cardiovascular effects are associated with both diesel exhaust and road traffic noise, but these exposures are hard to disentangle epidemiologically. We used an experimental setup to evaluate the impact of diesel exhaust particles and traffic noise, alone and combined, on intermediary outcomes related to the autonomic nervous system and increased cardiovascular risk. Methods In a controlled chamber 18 healthy adults were exposed to four scenarios in a randomized cross-over fashion. Each exposure scenario consisted of either filtered (clean) air or diesel engine exhaust (particle mass concentrations around 300 µg/m3), and either low (46 dB(A)) or high (75 dB(A)) levels of traffic noise for 3 h at rest. ECG was recorded for 10-min periods before and during each exposure type, and frequency-domain heart rate variability (HRV) computed. Endothelial dysfunction and arterial stiffness were assessed after each exposure using EndoPAT 2000. Results Compared to control exposure, HRV in the high frequency band decreased during exposure to diesel exhaust, both alone and combined with noise, but not during noise exposure only. These differences were more pronounced in women. We observed no synergistic effects of combined exposure, and no significant differences between exposure scenarios for other HRV indices, endothelial function or arterial stiffness. Conclusion Three-hour exposure to diesel exhaust, but not noise, was associated with decreased HRV in the high frequency band. This indicates activation of irritant receptor-mediated autonomic reflexes, a possible mechanism for the cardiovascular risks of diesel exposure. There was no effect on endothelial dysfunction or arterial stiffness after exposure.
{"title":"A controlled chamber study of effects of exposure to diesel exhaust particles and noise on heart rate variability and endothelial function","authors":"L. Stockfelt, Yiyi Xu, A. Gudmundsson, J. Rissler, C. Isaxon, J. Brunskog, J. Pagels, P. Nilsson, M. Berglund, L. Barregard, M. Bohgard, M. Albin, I. Hagerman, A. Wierzbicka","doi":"10.1080/08958378.2022.2065388","DOIUrl":"https://doi.org/10.1080/08958378.2022.2065388","url":null,"abstract":"Abstract Background Adverse cardiovascular effects are associated with both diesel exhaust and road traffic noise, but these exposures are hard to disentangle epidemiologically. We used an experimental setup to evaluate the impact of diesel exhaust particles and traffic noise, alone and combined, on intermediary outcomes related to the autonomic nervous system and increased cardiovascular risk. Methods In a controlled chamber 18 healthy adults were exposed to four scenarios in a randomized cross-over fashion. Each exposure scenario consisted of either filtered (clean) air or diesel engine exhaust (particle mass concentrations around 300 µg/m3), and either low (46 dB(A)) or high (75 dB(A)) levels of traffic noise for 3 h at rest. ECG was recorded for 10-min periods before and during each exposure type, and frequency-domain heart rate variability (HRV) computed. Endothelial dysfunction and arterial stiffness were assessed after each exposure using EndoPAT 2000. Results Compared to control exposure, HRV in the high frequency band decreased during exposure to diesel exhaust, both alone and combined with noise, but not during noise exposure only. These differences were more pronounced in women. We observed no synergistic effects of combined exposure, and no significant differences between exposure scenarios for other HRV indices, endothelial function or arterial stiffness. Conclusion Three-hour exposure to diesel exhaust, but not noise, was associated with decreased HRV in the high frequency band. This indicates activation of irritant receptor-mediated autonomic reflexes, a possible mechanism for the cardiovascular risks of diesel exposure. There was no effect on endothelial dysfunction or arterial stiffness after exposure.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42767307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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