首页 > 最新文献

International Journal of Clinical Practice最新文献

英文 中文
Percutaneous Coronary Intervention with Procedural Unfractionated Heparin without Activated Clotting Time Guidance: A Unique Opportunity to Assess Thrombotic and Bleeding Events 在无活化凝血时间指导下使用程序性非分叶肝素进行经皮冠状动脉介入治疗:评估血栓和出血事件的独特机会
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-02-15 DOI: 10.1155/2024/6219301
Ali Z. Zgheib, Jennifer Jdaidani, Elie Akl, Suzan Khalil, Omar Chaabo, Nicolo Piazza, Fadi J. Sawaya, Abdallah G. Rebeiz

Background. Rates of major bleeding and intraprocedural thrombotic events (IPTE) in the setting of percutaneous coronary intervention (PCI) using weight-adjusted unfractionated heparin (UFH) without activated clotting time (ACT) monitoring are not known. Methods. We reviewed 2,748 consecutive patients who underwent coronary angiography at our tertiary care university hospital between January 2017 and December 2020. All patients who underwent PCI with weight-adjusted UFH without ACT guidance were considered for further analysis. Major bleeding complications occurring within 48 hours of PCI were collected from patients’ medical records. IPTE were collected independently by two interventional cardiologists after review of coronary angiograms. Results. There were 718 patients included in the analysis (65.4 ± 12.2 years old; 81.3% male). In total, 45 patients (7.8%) experienced a major bleed or IPTE. The most common IPTE were slow/no reflow (1.5%) and coronary artery dissection with decreased flow (1.1%). Other IPTE occurred in <1% of cases. Major bleeding occurred in 11 patients (1.5%), of whom 8 required blood transfusion and 3 required vascular intervention. Bleeding complications were more common with femoral compared with radial access (6.6% vs. 0.2%, P < 0.001). Conclusion. Weight-adjusted UFH use during PCI without ACT monitoring was related to low rates of major bleeding or IPTE.

背景。在经皮冠状动脉介入治疗(PCI)中使用重量调整型非分叶肝素(UFH)而不进行活化凝血时间(ACT)监测时,大出血和术中血栓事件(IPTE)的发生率尚不清楚。方法。我们回顾了 2017 年 1 月至 2020 年 12 月期间在我们的三级医疗大学医院接受冠状动脉造影术的 2748 名连续患者。所有在没有 ACT 指导的情况下使用体重调整型 UFH 进行 PCI 的患者均被纳入进一步分析的考虑范围。PCI术后48小时内发生的主要出血并发症由患者病历收集。IPTE 由两名介入心脏病专家在查看冠状动脉造影后独立收集。结果。共有 718 名患者(65.4 ± 12.2 岁;81.3% 为男性)纳入分析。共有 45 名患者(7.8%)发生大出血或 IPTE。最常见的 IPTE 是血流缓慢/无回流(1.5%)和血流减少的冠状动脉夹层(1.1%)。其他 IPTE 发生率为 1%。11名患者(1.5%)出现大出血,其中8人需要输血,3人需要血管介入治疗。与桡动脉入路相比,股动脉入路的出血并发症更为常见(6.6% 对 0.2%)。结论在不进行ACT监测的PCI期间使用体重调整后的UFH与大出血或IPTE的低发生率有关。
{"title":"Percutaneous Coronary Intervention with Procedural Unfractionated Heparin without Activated Clotting Time Guidance: A Unique Opportunity to Assess Thrombotic and Bleeding Events","authors":"Ali Z. Zgheib,&nbsp;Jennifer Jdaidani,&nbsp;Elie Akl,&nbsp;Suzan Khalil,&nbsp;Omar Chaabo,&nbsp;Nicolo Piazza,&nbsp;Fadi J. Sawaya,&nbsp;Abdallah G. Rebeiz","doi":"10.1155/2024/6219301","DOIUrl":"10.1155/2024/6219301","url":null,"abstract":"<p><i>Background</i>. Rates of major bleeding and intraprocedural thrombotic events (IPTE) in the setting of percutaneous coronary intervention (PCI) using weight-adjusted unfractionated heparin (UFH) without activated clotting time (ACT) monitoring are not known. <i>Methods</i>. We reviewed 2,748 consecutive patients who underwent coronary angiography at our tertiary care university hospital between January 2017 and December 2020. All patients who underwent PCI with weight-adjusted UFH without ACT guidance were considered for further analysis. Major bleeding complications occurring within 48 hours of PCI were collected from patients’ medical records. IPTE were collected independently by two interventional cardiologists after review of coronary angiograms. <i>Results</i>. There were 718 patients included in the analysis (65.4 ± 12.2 years old; 81.3% male). In total, 45 patients (7.8%) experienced a major bleed or IPTE. The most common IPTE were slow/no reflow (1.5%) and coronary artery dissection with decreased flow (1.1%). Other IPTE occurred in &lt;1% of cases. Major bleeding occurred in 11 patients (1.5%), of whom 8 required blood transfusion and 3 required vascular intervention. Bleeding complications were more common with femoral compared with radial access (6.6% vs. 0.2%, <i>P</i> &lt; 0.001). <i>Conclusion</i>. Weight-adjusted UFH use during PCI without ACT monitoring was related to low rates of major bleeding or IPTE.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139758402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Psychometric Study of the Student Evidence-Based Practice Scale S-EBPQ-Arabic Version for Use among Undergraduate Nursing Students 学生循证实践量表 S-EBPQ 阿拉伯语版在护理专业本科生中的心理测量研究
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-02-13 DOI: 10.1155/2024/6375596
Basmah F. Alharbi

Background. Previous studies have demonstrated the significance of evidence-based practice in improving patient care and outcomes. Therefore, integrating evidence-based practice into the health professions’ education curriculum has become a pedagogical priority. However, there is a lack of reliable and valid scales to measure students’ evidence-based practice usage, attitudes, knowledge, and skills in Arab countries. Aim. This study aims to examine the adapted Student Evidence-Based Practice Scale Questionnaire (S-EBPQ) validity at logical statistical level and reliability for use among students in Arabic context. Methods. This cross-sectional study included 233 undergraduate nursing students from a university in Saudi Arabia, who were recruited after translating and pilot testing the S-EBPQ. Three distinctive types of validity including conceptual, content, and face validity were assessed to determine the quality of the questionnaire items logically. Exploratory factor analyses were performed to examine the tool’s structural validity. Additionally, internal consistency was assessed to evaluate reliability. Findings. All items were considered relevant to Arab culture, and no changes were made to any items. The content validity indices for all items were above 0.80 as this was considered an acceptable value. The exploratory factor analysis identified the same four factors (practice, attitude, retrieving and reviewing evidence, and sharing and applying evidence-based practice). All KMO values for the individual items ≥0.876 were also well above the acceptable 0.6 limit. The four-factor structure explained a total variance of 64%, with factor load score λ ≥ 0.455. The total and subscale S-EBPQ scores showed evidence of reliability, with Cronbach’s alpha ≥0.8. Conclusions. This study demonstrated the reliability and validity of the Arabic S-EBPQ version. The study has the potential to advance Arab countries’ understanding of evidence-based practice. S-EBPQ is a validated tool that can be used to assess nursing students’ knowledge of EBP practices. Since educators need to continually evaluate instructional and curricular design in order to meet contemporary nursing needs, this scale can enhance the educational process and enhance students’ competencies.

背景。以往的研究表明,循证实践在改善患者护理和治疗效果方面具有重要意义。因此,将循证实践纳入卫生专业教育课程已成为教学重点。然而,阿拉伯国家缺乏可靠有效的量表来衡量学生的循证实践使用情况、态度、知识和技能。研究目的本研究旨在检查经改编的学生循证实践量表问卷(S-EBPQ)在阿拉伯语环境下在学生中使用时在逻辑统计层面的有效性和可靠性。研究方法。这项横断面研究包括来自沙特阿拉伯一所大学的 233 名护理专业本科生。评估了三种不同类型的效度,包括概念效度、内容效度和表面效度,以确定问卷项目的逻辑质量。我们还进行了探索性因素分析,以检查工具的结构效度。此外,还评估了内部一致性以评价可靠性。调查结果所有项目都被认为与阿拉伯文化相关,没有对任何项目进行修改。所有项目的内容效度指数都高于 0.80,这被认为是一个可以接受的值。探索性因子分析确定了相同的四个因子(实践、态度、检索和审查证据以及分享和应用循证实践)。各单项的 KMO 值均≥0.876,也远远高于可接受的 0.6 限制。四因子结构解释了 64% 的总方差,因子载荷得分 λ ≥ 0.455。S-EBPQ的总分和分量表得分显示出可靠性,Cronbach's alpha≥0.8。结论本研究证明了阿拉伯语 S-EBPQ 版本的可靠性和有效性。该研究有可能促进阿拉伯国家对循证实践的理解。S-EBPQ 是一种经过验证的工具,可用于评估护理专业学生对 EBP 实践的了解程度。由于教育者需要不断评估教学和课程设计,以满足当代护理需求,因此该量表可以加强教育过程,提高学生的能力。
{"title":"A Psychometric Study of the Student Evidence-Based Practice Scale S-EBPQ-Arabic Version for Use among Undergraduate Nursing Students","authors":"Basmah F. Alharbi","doi":"10.1155/2024/6375596","DOIUrl":"10.1155/2024/6375596","url":null,"abstract":"<p><i>Background</i>. Previous studies have demonstrated the significance of evidence-based practice in improving patient care and outcomes. Therefore, integrating evidence-based practice into the health professions’ education curriculum has become a pedagogical priority. However, there is a lack of reliable and valid scales to measure students’ evidence-based practice usage, attitudes, knowledge, and skills in Arab countries. <i>Aim</i>. This study aims to examine the adapted Student Evidence-Based Practice Scale Questionnaire (S-EBPQ) validity at logical statistical level and reliability for use among students in Arabic context. <i>Methods</i>. This cross-sectional study included 233 undergraduate nursing students from a university in Saudi Arabia, who were recruited after translating and pilot testing the S-EBPQ. Three distinctive types of validity including conceptual, content, and face validity were assessed to determine the quality of the questionnaire items logically. Exploratory factor analyses were performed to examine the tool’s structural validity. Additionally, internal consistency was assessed to evaluate reliability. <i>Findings</i>. All items were considered relevant to Arab culture, and no changes were made to any items. The content validity indices for all items were above 0.80 as this was considered an acceptable value. The exploratory factor analysis identified the same four factors (practice, attitude, retrieving and reviewing evidence, and sharing and applying evidence-based practice). All KMO values for the individual items ≥0.876 were also well above the acceptable 0.6 limit. The four-factor structure explained a total variance of 64%, with factor load score <i>λ</i> ≥ 0.455. The total and subscale S-EBPQ scores showed evidence of reliability, with Cronbach’s alpha ≥0.8. <i>Conclusions</i>. This study demonstrated the reliability and validity of the Arabic S-EBPQ version. The study has the potential to advance Arab countries’ understanding of evidence-based practice. S-EBPQ is a validated tool that can be used to assess nursing students’ knowledge of EBP practices. Since educators need to continually evaluate instructional and curricular design in order to meet contemporary nursing needs, this scale can enhance the educational process and enhance students’ competencies.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139758467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Efficacy and Safety of First-Line Treatment Options for Unresectable Stage III Non-Small Cell Lung Cancer: A Retrospective Analysis 无法切除的 III 期非小细胞肺癌一线治疗方案的疗效和安全性比较:回顾性分析
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-02-12 DOI: 10.1155/2024/8585035
Luqing Zhao, Zhiting Zhao, Xiaoqi Yan, Fei Wu, Ning Sun, Renhong Guo, Shaorong Yu, Xiao Hu, Jifeng Feng

Background. Based on PACIFIC trial, durvalumab as consolidation therapy following concurrent chemoradiotherapy (cCRT) has been a new standard treatment for unresectable stage III non-small cell lung cancer (NSCLC). In clinical applications, there are heterogeneous adjustments or novel strategies following specialized discussions in experienced multidisciplinary teams. This study retrospectively compared the efficacy and safety of different first-line treatments for unresectable stage III NSCLC. Methods. We retrospectively analyzed 397 patients who received first-line treatment for unresectable stage III NSCLC. Comparisons and statistical analyses of treatment were made in terms of efficacy and safety. Adverse events and responses were assessed using CTCAE v5.0 and RECIST v1.1. The progression-free survival (PFS) was estimated using the Kaplan–Meier method or the Cox survival regression model and compared using the log-rank test. Results. In wild-type driver genes group, the objective response rate (ORR), disease control rate (DCR), and median PFS (mPFS) were prolonged in the radiotherapy group compared to those in the nonradiotherapy group (ORR: 50.94% vs. 30.06%, p < 0.001; DCR: 98.11% vs. 80.37%, p < 0.001; and mPFS: 21.00 vs. 8.20 months, p < 0.001). The incidence of pneumonia at any grade in the radiotherapy group was higher than that in the nonradiotherapy group (9.43% vs. 2.45%, p = 0.008). In the radiotherapy group, the chemoradiotherapy (CRT) plus immunotherapy subgroup had longer mPFS than the CRT subgroup, with increased toxicity at any grade (24.60 vs. 17.90 months, p = 0.025, and 83.17% vs. 65.52%, p = 0.011). In the nonradiotherapy group, the DCR and mPFS were higher in the chemotherapy plus immunotherapy subgroup than in the chemotherapy subgroup, with increased toxicity at any grade (DCR: 93.67% vs. 67.86%, p < 0.001; mPFS: 13.53 vs. 5.07 months, p < 0.001; and 68.35% vs. 41.67%, p = 0.001). In the mutant driver genes group, the efficacy did not significantly differ among the radiotherapy subgroup, targeted therapy subgroup, and radiotherapy plus targeted therapy subgroup (ORR: p = 0.633; mPFS: p = 0.450). Conclusions. For unresectable stage III NSCLC patients with wild-type driver genes, the combination of radiotherapy and immunotherapy in the initial treatment was essential to significantly improve the efficacy. For patients with mutant driver genes, radiotherapy, targeted therapy, and the combination of radiotherapy and targeted therapy showed similar short-term efficacy.

背景。根据 PACIFIC 试验,durvalumab 作为同期化放疗(cCRT)后的巩固治疗已成为不可切除的 III 期非小细胞肺癌(NSCLC)的新标准治疗方法。在临床应用中,经过经验丰富的多学科团队的专门讨论,出现了不同的调整或新策略。本研究回顾性比较了不同一线治疗方法对不可切除的 III 期 NSCLC 的疗效和安全性。方法。我们对接受一线治疗的 397 例不可切除 III 期 NSCLC 患者进行了回顾性分析。从疗效和安全性方面对治疗进行了比较和统计分析。不良事件和反应采用 CTCAE v5.0 和 RECIST v1.1 进行评估。采用 Kaplan-Meier 法或 Cox 生存回归模型估算无进展生存期(PFS),并采用 log-rank 检验进行比较。结果在野生型驱动基因组中,与非放疗组相比,放疗组的客观反应率(ORR)、疾病控制率(DCR)和中位生存期(mPFS)均有所延长(ORR:50.94% vs. 30.06%,< 0.001;DCR:98.11% vs. 80.37%,< 0.001;mPFS:21.00 vs. 8.20个月,< 0.001)。放疗组任何级别肺炎的发生率均高于非放疗组(9.43% vs. 2.45%,= 0.008)。在放疗组中,化学放疗(CRT)加免疫治疗亚组的mPFS比CRT亚组长,但任何级别的毒性均有所增加(24.60个月对17.90个月,P=0.025;83.17%对65.52%,=0.011)。在非放疗组中,化疗加免疫治疗亚组的 DCR 和 mPFS 均高于化疗亚组,但任何级别的毒性均有所增加(DCR:93.67% vs. 67.86%,< 0.001;mPFS:13.53 vs. 5.07 个月,< 0.001;68.35% vs. 41.67%,= 0.001)。在突变驱动基因组中,放疗亚组、靶向治疗亚组和放疗加靶向治疗亚组的疗效无明显差异(ORR:=0.633;mPFS:=0.450)。结论对于具有野生型驱动基因的不可切除的III期NSCLC患者,在初始治疗中联合放疗和免疫治疗对显著提高疗效至关重要。对于有突变驱动基因的患者,放疗、靶向治疗以及放疗与靶向治疗的联合治疗显示出相似的短期疗效。
{"title":"Comparison of Efficacy and Safety of First-Line Treatment Options for Unresectable Stage III Non-Small Cell Lung Cancer: A Retrospective Analysis","authors":"Luqing Zhao,&nbsp;Zhiting Zhao,&nbsp;Xiaoqi Yan,&nbsp;Fei Wu,&nbsp;Ning Sun,&nbsp;Renhong Guo,&nbsp;Shaorong Yu,&nbsp;Xiao Hu,&nbsp;Jifeng Feng","doi":"10.1155/2024/8585035","DOIUrl":"10.1155/2024/8585035","url":null,"abstract":"<p><i>Background</i>. Based on PACIFIC trial, durvalumab as consolidation therapy following concurrent chemoradiotherapy (cCRT) has been a new standard treatment for unresectable stage III non-small cell lung cancer (NSCLC). In clinical applications, there are heterogeneous adjustments or novel strategies following specialized discussions in experienced multidisciplinary teams. This study retrospectively compared the efficacy and safety of different first-line treatments for unresectable stage III NSCLC. <i>Methods</i>. We retrospectively analyzed 397 patients who received first-line treatment for unresectable stage III NSCLC. Comparisons and statistical analyses of treatment were made in terms of efficacy and safety. Adverse events and responses were assessed using CTCAE v5.0 and RECIST v1.1. The progression-free survival (PFS) was estimated using the Kaplan–Meier method or the Cox survival regression model and compared using the log-rank test. <i>Results</i>. In wild-type driver genes group, the objective response rate (ORR), disease control rate (DCR), and median PFS (mPFS) were prolonged in the radiotherapy group compared to those in the nonradiotherapy group (ORR: 50.94% vs. 30.06%, <i>p</i> &lt; 0.001; DCR: 98.11% vs. 80.37%, <i>p</i> &lt; 0.001; and mPFS: 21.00 vs. 8.20 months, <i>p</i> &lt; 0.001). The incidence of pneumonia at any grade in the radiotherapy group was higher than that in the nonradiotherapy group (9.43% vs. 2.45%, <i>p</i> = 0.008). In the radiotherapy group, the chemoradiotherapy (CRT) plus immunotherapy subgroup had longer mPFS than the CRT subgroup, with increased toxicity at any grade (24.60 vs. 17.90 months, p = 0.025, and 83.17% vs. 65.52%, <i>p</i> = 0.011). In the nonradiotherapy group, the DCR and mPFS were higher in the chemotherapy plus immunotherapy subgroup than in the chemotherapy subgroup, with increased toxicity at any grade (DCR: 93.67% vs. 67.86%, <i>p</i> &lt; 0.001; mPFS: 13.53 vs. 5.07 months, <i>p</i> &lt; 0.001; and 68.35% vs. 41.67%, <i>p</i> = 0.001). In the mutant driver genes group, the efficacy did not significantly differ among the radiotherapy subgroup, targeted therapy subgroup, and radiotherapy plus targeted therapy subgroup (ORR: <i>p</i> = 0.633; mPFS: <i>p</i> = 0.450). <i>Conclusions</i>. For unresectable stage III NSCLC patients with wild-type driver genes, the combination of radiotherapy and immunotherapy in the initial treatment was essential to significantly improve the efficacy. For patients with mutant driver genes, radiotherapy, targeted therapy, and the combination of radiotherapy and targeted therapy showed similar short-term efficacy.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139758407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Network Pharmacology Analysis of the Therapeutic Potential of Colchicine in Acute Lung Injury 秋水仙碱对急性肺损伤治疗潜力的网络药理学分析
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-02-06 DOI: 10.1155/2024/9940182
Fei Sun, Lijuan Zhang, Lulu Shen, Chunman Wang

Background. This study employed integrated network pharmacology approach to explore the mechanisms underlying the protective effect of colchicine against acute lung injury (ALI). Methods. We analyzed the expression profiles from 13 patients with sepsis-related ALI and 21 controls to identify differentially expressed genes and key modules. ALI-related genes were curated using databases such as DisGeNET, Therapeutic Target, and Comparative Toxicogenomics Database to curate ALI-related genes. Drug target fishing for colchicine was conducted using the DrugBank, BATMAN-TCM, STITCH, and SwissTargetPrediction. Potential drug-disease interactions were determined by intersecting ALI-associated genes with colchicine target genes. We performed comprehensive pathway and process enrichment analyses on these genes. A protein-protein interaction network was constructed, and topological analysis was executed. Additionally, an ALI mouse model was established to evaluate the effect of colchicine on CXCL12 and CXCR4 levels through western blot analysis. Results. Analysis revealed 23 potential colchicine-ALI interaction genes from the intersection of 253 ALI-associated genes and 389 colchicine targets. Functional enrichment analysis highlighted several inflammation-related pathways, such as cytokine-mediated signaling pathway, CXCR chemokine receptor binding, NF-kappa B signaling pathway, TNF signaling pathway, and IL-17 signaling pathway. The protein-protein interaction network demonstrated complex interactions for CXCL12 and CXCR4 among other candidate genes, with significant topological interaction degrees. In vivo studies showed that colchicine significantly reduced elevated CXCL12 and CXCR4 levels in ALI mice. Conclusion. Our findings suggest that colchicine’s therapeutic effect on ALI might derive from its anti-inflammatory properties. Further research is needed to explore the specific mechanisms of colchicine’s interaction with sepsis-induced ALI.

背景:本研究采用整合网络药理学方法探讨秋水仙碱对急性肺损伤(ALI)的保护作用机制:本研究采用整合网络药理学方法探讨秋水仙碱对急性肺损伤(ALI)的保护作用机制:我们分析了13例脓毒症相关ALI患者和21例对照组的表达谱,以确定差异表达基因和关键模块。我们利用 DisGeNET、Therapeutic Target 和比较毒物基因组学数据库等数据库对 ALI 相关基因进行了整理。使用 DrugBank、BATMAN-TCM、STITCH 和 SwissTargetPrediction 对秋水仙碱进行药物靶点筛选。通过将 ALI 相关基因与秋水仙碱靶基因交叉,确定了潜在的药物-疾病相互作用。我们对这些基因进行了全面的通路和过程富集分析。我们构建了蛋白质-蛋白质相互作用网络,并进行了拓扑分析。此外,我们还建立了 ALI 小鼠模型,通过 Western 印迹分析评估秋水仙碱对 CXCL12 和 CXCR4 水平的影响:结果:从253个ALI相关基因和389个秋水仙碱靶点的交叉点中,分析发现了23个潜在的秋水仙碱-ALI相互作用基因。功能富集分析强调了几个炎症相关通路,如细胞因子介导的信号通路、CXCR趋化因子受体结合、NF-kappa B 信号通路、TNF 信号通路和 IL-17 信号通路。蛋白-蛋白相互作用网络显示,CXCL12和CXCR4与其他候选基因之间存在复杂的相互作用,并具有显著的拓扑相互作用度。体内研究表明,秋水仙碱能显著降低 ALI 小鼠体内升高的 CXCL12 和 CXCR4 水平:我们的研究结果表明,秋水仙碱对 ALI 的治疗作用可能来自于其抗炎特性。我们需要进一步研究秋水仙碱与脓毒症诱导的 ALI 相互作用的具体机制。
{"title":"Network Pharmacology Analysis of the Therapeutic Potential of Colchicine in Acute Lung Injury","authors":"Fei Sun,&nbsp;Lijuan Zhang,&nbsp;Lulu Shen,&nbsp;Chunman Wang","doi":"10.1155/2024/9940182","DOIUrl":"10.1155/2024/9940182","url":null,"abstract":"<p><i>Background</i>. This study employed integrated network pharmacology approach to explore the mechanisms underlying the protective effect of colchicine against acute lung injury (ALI). <i>Methods</i>. We analyzed the expression profiles from 13 patients with sepsis-related ALI and 21 controls to identify differentially expressed genes and key modules. ALI-related genes were curated using databases such as DisGeNET, Therapeutic Target, and Comparative Toxicogenomics Database to curate ALI-related genes. Drug target fishing for colchicine was conducted using the DrugBank, BATMAN-TCM, STITCH, and SwissTargetPrediction. Potential drug-disease interactions were determined by intersecting ALI-associated genes with colchicine target genes. We performed comprehensive pathway and process enrichment analyses on these genes. A protein-protein interaction network was constructed, and topological analysis was executed. Additionally, an ALI mouse model was established to evaluate the effect of colchicine on CXCL12 and CXCR4 levels through western blot analysis. <i>Results</i>. Analysis revealed 23 potential colchicine-ALI interaction genes from the intersection of 253 ALI-associated genes and 389 colchicine targets. Functional enrichment analysis highlighted several inflammation-related pathways, such as cytokine-mediated signaling pathway, CXCR chemokine receptor binding, NF-kappa B signaling pathway, TNF signaling pathway, and IL-17 signaling pathway. The protein-protein interaction network demonstrated complex interactions for CXCL12 and CXCR4 among other candidate genes, with significant topological interaction degrees. <i>In vivo</i> studies showed that colchicine significantly reduced elevated CXCL12 and CXCR4 levels in ALI mice. <i>Conclusion</i>. Our findings suggest that colchicine’s therapeutic effect on ALI might derive from its anti-inflammatory properties. Further research is needed to explore the specific mechanisms of colchicine’s interaction with sepsis-induced ALI.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139729558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Different Inspiratory Muscle Training Protocols on Exercise Capacity, Respiratory Muscle Strength, and Health-Related Quality of Life in Patients with Hypertension 不同吸气肌训练方案对高血压患者运动能力、呼吸肌力量和健康相关生活质量的影响
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-02-03 DOI: 10.1155/2024/4136457
İrem Hüzmeli, Nihan Katayıfçı, Fatih Yalçın, Esra Doğru Hüzmeli

Aim. This study aimed to explore how varying inspiratory muscle training workloads affect exercise capacity, health-related quality of life (HrQoL), depression, peripheral and respiratory muscle strength, pulmonary function, dyspnea, fatigue, and physical activity levels in hypertension (HT) patients. Methods. A randomized, controlled three-arm study. Forty-five patients (58.37 ± 8.53 y, 7F/38M) with HT received IMT (7 days/8 weeks) by POWERbreathe® Classic LR device and were randomized to control group (CG, 10% maximal inspiratory pressure (MIP), n: 15), low-load group (LLG, 30% MIP), and high-load group (HLG, %50 MIP). Exercise capacity, HrQoL, depression, peripheral and respiratory muscle strength, pulmonary function, fatigue, physical activity level, dyspnea, and sleep quality were evaluated before and after the training. Results. Exercise capacity, physical functioning, peripheral muscle strength, and resting dyspnea were statistically significantly improved in HLG and LLG after the training compared to CG (p < 0.05). Similar improvements in perception of depression, fatigue, and sleep quality were seen within and between the groups (p > 0.05). Statistically significant differences were found within all the groups in terms of MIP and PEF values of respiratory functions (p < 0.05). The superior improvement in the physical activity level was found in the HLG (p < 0.05). Discussion. High-load IMT was particularly effective in increasing physical activity level, peripheral muscle strength, exercise capacity, and improved HrQoL. Low-load IMT was effective in reducing dyspnea and improving respiratory function. Device-guided breathing exercises decreased blood pressure, improved sleep quality, and strengthened respiratory muscles. IMT, an efficient method, is suggested for inclusion in rehabilitation programs due to its capacity to increase physical activity, exercise capacity, and peripheral muscle strength, enhance HrQoL and respiratory function, and alleviate dyspnea. Also, the efficacy of IMT should be investigated with different training protocols such as endurance IMT or functional IMT in HT patients.

研究目的本研究旨在探讨不同的吸气肌肉训练工作量如何影响高血压(HT)患者的运动能力、健康相关生活质量(HrQoL)、抑郁、外周和呼吸肌力量、肺功能、呼吸困难、疲劳和体力活动水平。研究方法随机对照三臂研究。45 名高血压患者(58.37 ± 8.53 岁,7F/38M)通过 POWERbreathe® Classic LR 设备接受 IMT(7 天/8 周),并随机分为对照组(CG,10% 最大吸气压力(MIP),n:15)、低负荷组(LLG,30% MIP)和高负荷组(HLG,%50 MIP)。对训练前后的运动能力、HrQoL、抑郁、外周和呼吸肌力量、肺功能、疲劳、体力活动水平、呼吸困难和睡眠质量进行了评估。结果与 CG 相比,HLG 和 LLG 在训练后的运动能力、身体机能、外周肌力和静息呼吸困难在统计学上有明显改善。组内和组间的抑郁感、疲劳感和睡眠质量也有类似的改善。在呼吸功能的 MIP 值和 PEF 值方面,所有组内的差异均有统计学意义。体力活动水平的提高在 HLG 组中更为明显。讨论高负荷 IMT 对提高体力活动水平、外周肌力、运动能力和改善 HrQoL 特别有效。低负荷 IMT 对减少呼吸困难和改善呼吸功能有效。设备引导的呼吸练习可降低血压、改善睡眠质量并增强呼吸肌。IMT是一种有效的方法,可增加体力活动、运动能力和外周肌肉力量,提高生活质量和呼吸功能,缓解呼吸困难,因此建议将其纳入康复计划。此外,还应该对高血压患者进行不同训练方案(如耐力性高血压综合训练或功能性高血压综合训练)的有效性研究。
{"title":"Effects of Different Inspiratory Muscle Training Protocols on Exercise Capacity, Respiratory Muscle Strength, and Health-Related Quality of Life in Patients with Hypertension","authors":"İrem Hüzmeli,&nbsp;Nihan Katayıfçı,&nbsp;Fatih Yalçın,&nbsp;Esra Doğru Hüzmeli","doi":"10.1155/2024/4136457","DOIUrl":"10.1155/2024/4136457","url":null,"abstract":"<p><i>Aim</i>. This study aimed to explore how varying inspiratory muscle training workloads affect exercise capacity, health-related quality of life (HrQoL), depression, peripheral and respiratory muscle strength, pulmonary function, dyspnea, fatigue, and physical activity levels in hypertension (HT) patients. <i>Methods</i>. A randomized, controlled three-arm study. Forty-five patients (58.37 ± 8.53 y, 7F/38M) with HT received IMT (7 days/8 weeks) by POWERbreathe® Classic LR device and were randomized to control group (CG, 10% maximal inspiratory pressure (MIP), <i>n</i>: 15), low-load group (LLG, 30% MIP), and high-load group (HLG, %50 MIP). Exercise capacity, HrQoL, depression, peripheral and respiratory muscle strength, pulmonary function, fatigue, physical activity level, dyspnea, and sleep quality were evaluated before and after the training. <i>Results</i>. Exercise capacity, physical functioning, peripheral muscle strength, and resting dyspnea were statistically significantly improved in HLG and LLG after the training compared to CG (<i>p</i> &lt; 0.05). Similar improvements in perception of depression, fatigue, and sleep quality were seen within and between the groups (<i>p</i> &gt; 0.05). Statistically significant differences were found within all the groups in terms of MIP and PEF values of respiratory functions (<i>p</i> &lt; 0.05). The superior improvement in the physical activity level was found in the HLG (<i>p</i> &lt; 0.05). <i>Discussion</i>. High-load IMT was particularly effective in increasing physical activity level, peripheral muscle strength, exercise capacity, and improved HrQoL. Low-load IMT was effective in reducing dyspnea and improving respiratory function. Device-guided breathing exercises decreased blood pressure, improved sleep quality, and strengthened respiratory muscles. IMT, an efficient method, is suggested for inclusion in rehabilitation programs due to its capacity to increase physical activity, exercise capacity, and peripheral muscle strength, enhance HrQoL and respiratory function, and alleviate dyspnea. Also, the efficacy of IMT should be investigated with different training protocols such as endurance IMT or functional IMT in HT patients.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139669389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Community Pharmacists’ Knowledge and Perception towards Telepharmacy Services and Willingness to Practice It in Light of COVID-19 社区药剂师对远程药学服务的认识和看法以及根据 COVID-19 开展远程药学服务的意愿
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-01-31 DOI: 10.1155/2024/6656097
Ayesha Siddiqua, Soha Makki, Sazada Siddiqui, Randa A. Abdelkarim, Tahani Jubran, Wejdan Nwar, Ahlam Alqahtani, Maryam Alshehri, Maram Saeed, Arwa Khaled

Background. During the COVID-19 pandemic, there was increased adoption of telepharmacy, which has proven benefits. This study was conducted to assess the knowledge and perception of the community pharmacists of Aseer region, Saudi Arabia, towards telepharmacy services and evaluate their willingness to adopt telepharmacy in clinical practice during the COVID-19 pandemic. Materials and Methods. A cross-sectional study was conducted using an online self-administered structured anonymous questionnaire on the community pharmacists of Aseer region, Saudi Arabia. It covers demographics, computer access and literacy, knowledge and perception, and willingness to practice telepharmacy. Results were expressed as frequencies, percentages, and mean. The comparison between the classes of the demographic variables and the scores was done via Kruskal–Wallis and Mann–Whitney tests. Result. About half of the pharmacists in our study showed average knowledge about telepharmacy, the practical application of telepharmacy technology, and telepharmacy guidelines with a percentage of 53%, 52%, and 47%, respectively). Majority of the participants showed high perception towards telepharmacy in Saudi Arabia, while 93% of the pharmacists in Saudi Arabia agreed that the implementation of telepharmacy technology is appropriate due to the current COVID-19 pandemic. Only a significant relation was found between gender and computer access, literacy, and perceptions towards telepharmacy with p values of 0.033 and 0.026, respectively. Conclusion. The majority of the community pharmacists exhibited a positive perception and are willing to practice the concept of telepharmacy despite having a below average knowledge of telepharmacy. A future study involving the entire kingdom of Saudi Arabia could help identify the gaps in the knowledge, perception, and willingness to practice telepharmacy on a broader scale and thus promote telepharmacy adoption in the entire kingdom.

背景。在 COVID-19 大流行期间,采用远程药学的人数有所增加,远程药学的益处已得到证实。本研究旨在评估沙特阿拉伯 Aseer 地区社区药剂师对远程药学服务的了解和看法,并评估他们在 COVID-19 大流行期间在临床实践中采用远程药学的意愿。材料和方法。本研究采用在线自填式结构匿名问卷对沙特阿拉伯 Aseer 地区的社区药剂师进行了横断面研究。调查内容包括人口统计学、计算机使用和读写能力、知识和认知以及远程药学实践的意愿。结果以频率、百分比和平均值表示。通过 Kruskal-Wallis 和 Mann-Whitney 检验对人口统计学变量的等级和得分进行比较。结果在我们的研究中,约半数药剂师对远程药学、远程药学技术的实际应用和远程药学指南的了解程度一般,分别为 53%、52% 和 47%。)大多数参与者对沙特阿拉伯远程药学的认知度较高,93% 的沙特阿拉伯药剂师认为,由于目前 COVID-19 的流行,实施远程药学技术是适当的。仅发现性别与计算机使用、文化程度和对远程药学的认知之间存在明显关系,数值分别为 0.033 和 0.026。结论尽管大多数社区药剂师对远程药学的认识低于平均水平,但他们对远程药学的概念表现出积极的看法,并愿意付诸实践。未来一项涉及整个沙特阿拉伯王国的研究将有助于在更大范围内找出在远程药学的知识、认知和实践意愿方面存在的差距,从而促进远程药学在整个王国的应用。
{"title":"Community Pharmacists’ Knowledge and Perception towards Telepharmacy Services and Willingness to Practice It in Light of COVID-19","authors":"Ayesha Siddiqua,&nbsp;Soha Makki,&nbsp;Sazada Siddiqui,&nbsp;Randa A. Abdelkarim,&nbsp;Tahani Jubran,&nbsp;Wejdan Nwar,&nbsp;Ahlam Alqahtani,&nbsp;Maryam Alshehri,&nbsp;Maram Saeed,&nbsp;Arwa Khaled","doi":"10.1155/2024/6656097","DOIUrl":"10.1155/2024/6656097","url":null,"abstract":"<p><i>Background</i>. During the COVID-19 pandemic, there was increased adoption of telepharmacy, which has proven benefits. This study was conducted to assess the knowledge and perception of the community pharmacists of Aseer region, Saudi Arabia, towards telepharmacy services and evaluate their willingness to adopt telepharmacy in clinical practice during the COVID-19 pandemic. <i>Materials and Methods</i>. A cross-sectional study was conducted using an online self-administered structured anonymous questionnaire on the community pharmacists of Aseer region, Saudi Arabia. It covers demographics, computer access and literacy, knowledge and perception, and willingness to practice telepharmacy. Results were expressed as frequencies, percentages, and mean. The comparison between the classes of the demographic variables and the scores was done via Kruskal–Wallis and Mann–Whitney tests. <i>Result</i>. About half of the pharmacists in our study showed average knowledge about telepharmacy, the practical application of telepharmacy technology, and telepharmacy guidelines with a percentage of 53%, 52%, and 47%, respectively). Majority of the participants showed high perception towards telepharmacy in Saudi Arabia, while 93% of the pharmacists in Saudi Arabia agreed that the implementation of telepharmacy technology is appropriate due to the current COVID-19 pandemic. Only a significant relation was found between gender and computer access, literacy, and perceptions towards telepharmacy with <i>p</i> values of 0.033 and 0.026, respectively. <i>Conclusion</i>. The majority of the community pharmacists exhibited a positive perception and are willing to practice the concept of telepharmacy despite having a below average knowledge of telepharmacy. A future study involving the entire kingdom of Saudi Arabia could help identify the gaps in the knowledge, perception, and willingness to practice telepharmacy on a broader scale and thus promote telepharmacy adoption in the entire kingdom.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Machine Learning-Based Integrated Analysis of PANoptosis Patterns in Acute Myeloid Leukemia Reveals a Signature Predicting Survival and Immunotherapy 基于机器学习的急性髓性白血病泛凋亡模式综合分析揭示了预测生存和免疫疗法的特征
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-01-30 DOI: 10.1155/2024/5113990
Lanlan Tang, Wei Zhang, Yang Zhang, Wenjun Deng, Mingyi Zhao

Objective. We conducted a meticulous bioinformatics analysis leveraging expression data of 226 PANRGs obtained from previous studies, as well as clinical data from AML patients derived from the HOVON database. Methods. Through meticulous data analysis and manipulation, we were able to categorize AML cases into two distinct PANRG clusters and subsequently identify differentially expressed genes (PRDEGs) with prognostic significance. Furthermore, we organized the patient data into two corresponding gene clusters, allowing us to investigate the intricate relationship between the risk score, patient prognosis, and the immune landscape. Results. Our findings disclosed significant associations between the identified PANRGs, gene clusters, patient survival, immune system, and cancer-related biological processes and pathways. Importantly, we successfully constructed a prognostic signature comprising nineteen genes, enabling the stratification of patients into high-risk and low-risk groups based on individually calculated risk scores. Furthermore, we developed a robust and practical nomogram model, integrating the risk score and other pertinent clinical features, to facilitate accurate patient survival prediction. Our comprehensive analysis demonstrated that the high-risk group exhibited notably worse prognosis, with the risk score proving to be significantly correlated with infiltration of most immune cells. The qRT-PCR results revealed significant differential expression patterns of LGR5 and VSIG4 in normal and human leukemia cell lines (HL-60 and MV-4-11). Conclusions. Our findings underscore the potential utility of PANoptosis-based molecular clustering and prognostic signatures as predictive tools for assessing patient survival in AML.

目的。我们利用以往研究中获得的 226 个 PANRGs 的表达数据以及 HOVON 数据库中 AML 患者的临床数据进行了细致的生物信息学分析。方法。通过缜密的数据分析和处理,我们将急性髓细胞性白血病病例分为两个不同的 PANRG 群,并随后确定了具有预后意义的差异表达基因 (PRDEG)。此外,我们还将患者数据分为两个相应的基因群,从而研究了风险评分、患者预后和免疫状况之间错综复杂的关系。结果我们的研究结果表明,在已识别的 PANRGs、基因簇、患者生存、免疫系统以及癌症相关的生物过程和通路之间存在重大关联。重要的是,我们成功构建了由 19 个基因组成的预后特征,从而能够根据单独计算的风险评分将患者分为高风险组和低风险组。此外,我们还开发了一个稳健实用的提名图模型,将风险评分和其他相关临床特征整合在一起,以便准确预测患者的生存期。我们的综合分析表明,高风险组的预后明显较差,风险评分与大多数免疫细胞的浸润有显著相关性。qRT-PCR 结果显示,LGR5 和 VSIG4 在正常细胞系和人类白血病细胞系(HL-60 和 MV-4-11)中的表达模式存在明显差异。结论。我们的研究结果强调了基于 PANoptosis 的分子聚类和预后特征作为评估急性髓细胞性白血病患者存活率的预测工具的潜在用途。
{"title":"Machine Learning-Based Integrated Analysis of PANoptosis Patterns in Acute Myeloid Leukemia Reveals a Signature Predicting Survival and Immunotherapy","authors":"Lanlan Tang,&nbsp;Wei Zhang,&nbsp;Yang Zhang,&nbsp;Wenjun Deng,&nbsp;Mingyi Zhao","doi":"10.1155/2024/5113990","DOIUrl":"10.1155/2024/5113990","url":null,"abstract":"<p><i>Objective</i>. We conducted a meticulous bioinformatics analysis leveraging expression data of 226 PANRGs obtained from previous studies, as well as clinical data from AML patients derived from the HOVON database. <i>Methods</i>. Through meticulous data analysis and manipulation, we were able to categorize AML cases into two distinct PANRG clusters and subsequently identify differentially expressed genes (PRDEGs) with prognostic significance. Furthermore, we organized the patient data into two corresponding gene clusters, allowing us to investigate the intricate relationship between the risk score, patient prognosis, and the immune landscape. <i>Results</i>. Our findings disclosed significant associations between the identified PANRGs, gene clusters, patient survival, immune system, and cancer-related biological processes and pathways. Importantly, we successfully constructed a prognostic signature comprising nineteen genes, enabling the stratification of patients into high-risk and low-risk groups based on individually calculated risk scores. Furthermore, we developed a robust and practical nomogram model, integrating the risk score and other pertinent clinical features, to facilitate accurate patient survival prediction. Our comprehensive analysis demonstrated that the high-risk group exhibited notably worse prognosis, with the risk score proving to be significantly correlated with infiltration of most immune cells. The qRT-PCR results revealed significant differential expression patterns of LGR5 and VSIG4 in normal and human leukemia cell lines (HL-60 and MV-4-11). <i>Conclusions</i>. Our findings underscore the potential utility of PANoptosis-based molecular clustering and prognostic signatures as predictive tools for assessing patient survival in AML.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139587316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GPX3-Mediated Oxidative Stress Affects Pyrimidine Metabolism Levels in Stomach Adenocarcinoma via the AMPK/mTOR Pathway GPX3 介导的氧化应激通过 AMPK/mTOR 通路影响胃腺癌的嘧啶代谢水平
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-01-30 DOI: 10.1155/2024/6875417
Yaowen Zhang, Yixin Yang, Shanshan Kuang, Yang Zhang, Hancheng Qin, Jisheng Xie

Background. Amino acid metabolism, including ATP production, nucleotide synthesis, and redox homeostatic processes, are associated with proliferation and differentiation of tumor cells. This study aimed to identify novel prognostic biomarkers and potential therapeutic targets of amino acid metabolism-related genes for stomach adenocarcinoma (STAD). Methods. RNA sequencing transcriptome data in the TCGA-STAD (training set) and GTEx datasets (validation set) were used. The LIMMA R program enabled the differentially expressed amino acid metabolism-related genes (AAMRGs) to be found. A prognostic risk score model based on clinical phenotypic features was built using LASSO regression and step multi-Cox analyses. Gene set enrichment analysis (GSEA) was used to find potential molecular pathways associated with STAD. Hierarchical cluster analysis was used to evaluate pyrimidine metabolism. Cultured STAD cells assessed the proliferation of STAD and upregulation of GPX3 expression by CCK8 and flow cytometry. Transwell and wound healing assays assessed the impact of GPX3 on invasion and migration of STAD cells. Western blot and qRT-PCR were used to measure changes in pyrimidine metabolism-related markers and active molecules involved in the AMPK/mTOR signaling pathway. Results. Three AAMRGs, DNMT1, F2R, and GPX3, could independently predict the course of STAD. Pyrimidine metabolism appeared to be significantly associated with these by GSEA and clustering analyses. Pyrimidine metabolism was negatively correlated with GPX3. Functional studies using an overexpressed GPX3 plasmid showed an enhanced migration and invasion of STAD cells as well as the expression of genes associated with pyrimidine metabolism and the AMPK/mTOR signaling pathway. By using a CAD siRNA, it was found that that GPX3 affected 5-fluorouracil resistance during de novo synthesis of pyrimidine through the CAD-UMPS signaling axis. Conclusions. GPX3 which regulates the level of pyrimidine metabolism through the AMPK/mTOR pathway was found to be closely associated with STAD. Our findings demonstrate GPX3 is a reliable biomarker for the prognosis of amino acid metabolism and a probable target for STAD therapy.

背景。氨基酸代谢,包括 ATP 生成、核苷酸合成和氧化还原平衡过程,与肿瘤细胞的增殖和分化有关。本研究旨在确定胃腺癌(STAD)氨基酸代谢相关基因的新型预后生物标志物和潜在治疗靶点。研究方法使用 TCGA-STAD 数据集(训练集)和 GTEx 数据集(验证集)中的 RNA 测序转录组数据。利用 LIMMA R 程序找到了差异表达的氨基酸代谢相关基因(AAMRGs)。利用 LASSO 回归和阶跃多 Cox 分析建立了基于临床表型特征的预后风险评分模型。基因组富集分析(GSEA)用于寻找与 STAD 相关的潜在分子通路。层次聚类分析用于评估嘧啶代谢。培养的 STAD 细胞通过 CCK8 和流式细胞术评估了 STAD 的增殖和 GPX3 表达的上调。透孔试验和伤口愈合试验评估了 GPX3 对 STAD 细胞侵袭和迁移的影响。Western 印迹和 qRT-PCR 被用来测量嘧啶代谢相关标记物和参与 AMPK/mTOR 信号通路的活性分子的变化。结果显示三种AAMRGs(DNMT1、F2R和GPX3)可独立预测STAD的病程。通过GSEA和聚类分析,嘧啶代谢似乎与这些因素有显著关联。嘧啶代谢与 GPX3 呈负相关。使用过表达 GPX3 质粒进行的功能研究显示,STAD 细胞的迁移和侵袭能力增强,与嘧啶代谢和 AMPK/mTOR 信号通路相关的基因表达也增强。通过使用 CAD siRNA,研究发现 GPX3 通过 CAD-UMPS 信号轴影响了嘧啶从头合成过程中的 5 氟尿嘧啶耐药性。结论通过 AMPK/mTOR 通路调节嘧啶代谢水平的 GPX3 被发现与 STAD 密切相关。我们的研究结果表明,GPX3 是氨基酸代谢预后的可靠生物标志物,也是 STAD 治疗的可能靶点。
{"title":"GPX3-Mediated Oxidative Stress Affects Pyrimidine Metabolism Levels in Stomach Adenocarcinoma via the AMPK/mTOR Pathway","authors":"Yaowen Zhang,&nbsp;Yixin Yang,&nbsp;Shanshan Kuang,&nbsp;Yang Zhang,&nbsp;Hancheng Qin,&nbsp;Jisheng Xie","doi":"10.1155/2024/6875417","DOIUrl":"10.1155/2024/6875417","url":null,"abstract":"<p><i>Background</i>. Amino acid metabolism, including ATP production, nucleotide synthesis, and redox homeostatic processes, are associated with proliferation and differentiation of tumor cells. This study aimed to identify novel prognostic biomarkers and potential therapeutic targets of amino acid metabolism-related genes for stomach adenocarcinoma (STAD). <i>Methods</i>. RNA sequencing transcriptome data in the TCGA-STAD (training set) and GTEx datasets (validation set) were used. The LIMMA R program enabled the differentially expressed amino acid metabolism-related genes (AAMRGs) to be found. A prognostic risk score model based on clinical phenotypic features was built using LASSO regression and step multi-Cox analyses. Gene set enrichment analysis (GSEA) was used to find potential molecular pathways associated with STAD. Hierarchical cluster analysis was used to evaluate pyrimidine metabolism. Cultured STAD cells assessed the proliferation of STAD and upregulation of GPX3 expression by CCK8 and flow cytometry. Transwell and wound healing assays assessed the impact of GPX3 on invasion and migration of STAD cells. Western blot and qRT-PCR were used to measure changes in pyrimidine metabolism-related markers and active molecules involved in the AMPK/mTOR signaling pathway. <i>Results</i>. Three AAMRGs, DNMT1, F2R, and GPX3, could independently predict the course of STAD. Pyrimidine metabolism appeared to be significantly associated with these by GSEA and clustering analyses. Pyrimidine metabolism was negatively correlated with GPX3. Functional studies using an overexpressed GPX3 plasmid showed an enhanced migration and invasion of STAD cells as well as the expression of genes associated with pyrimidine metabolism and the AMPK/mTOR signaling pathway. By using a CAD siRNA, it was found that that GPX3 affected 5-fluorouracil resistance during <i>de novo</i> synthesis of pyrimidine through the CAD-UMPS signaling axis. <i>Conclusions</i>. GPX3 which regulates the level of pyrimidine metabolism through the AMPK/mTOR pathway was found to be closely associated with STAD. Our findings demonstrate GPX3 is a reliable biomarker for the prognosis of amino acid metabolism and a probable target for STAD therapy.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139587396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mediterranean-Oriented Dietary Intervention Is Effective to Reduce Liver Steatosis in Patients with Nonalcoholic Fatty Liver Disease: Results from an Italian Clinical Trial 地中海式膳食干预能有效减轻非酒精性脂肪肝患者的肝脏脂肪变性:一项意大利临床试验的结果
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-01-25 DOI: 10.1155/2024/8861126
Barbara Zanini, Federica Benini, Monica Marullo, Anna Simonetto, Angelo Rossi, Paola Cavagnoli, Alessia Bonalumi, Silvia Marconi, Marie Graciella Pigozzi, Gianni Gilioli, Alessandra Valerio, Francesco Donato, Maurizio Castellano, Chiara Ricci

Background of the Study. Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in western countries. Lifestyle interventions are recommended as the primary therapy for NAFLD. Methodology. In this clinical trial, NAFLD patients were enrolled in a 12-month dietary intervention aimed to improve their eating habits according to the Mediterranean pattern, with scheduled appointments every three months. After the exclusion of steatosis, healthy subjects were recruited and received general advice based on current Italian food-based dietary guidelines. Results. One hundred and fifty five subjects aged 20–59 years underwent (i) liver ultrasound (US), (ii) clinical and anthropometric evaluations, (iii) blood tests, and (iv) assessment of dietary habits. According to US evaluation, 73 of them had severe, moderate, or mild liver steatosis (NAFLD patients) and 82 had no liver steatosis (healthy controls). Fifty-eight NAFLD patients and 73 controls completed the study. Among NAFLD patients, 26 (45%) downgraded steatosis severity, 12 of which achieved complete steatosis regression (21%). Three of the healthy controls developed NAFLD. The NAFLD patients improved their dietary habits and reduced BMI and waist circumference, during the study period, more than healthy controls. Liver steatosis remission/regression was independent of changes in BMI or liver enzymes and was more frequent among patients with mild steatosis at baseline. Conclusions. Mediterranean dietary advices, without a personalised meal planning, were efficient in reducing/remitting NAFLD, especially among patients with mild disease, which argues in favour of early identification and lifestyle intervention. This trial is registered with NCT03300661.

研究背景。非酒精性脂肪肝(NAFLD)是西方国家最常见的肝病。建议将生活方式干预作为治疗非酒精性脂肪肝的主要方法。研究方法在这项临床试验中,非酒精性脂肪肝患者参加了为期 12 个月的饮食干预,旨在按照地中海模式改善饮食习惯,每三个月预约一次。在排除脂肪肝后,招募健康受试者,并根据意大利现行的以食物为基础的饮食指南向他们提供一般性建议。结果155 名 20-59 岁的受试者接受了(i) 肝脏超声波检查 (US)、(ii) 临床和人体测量评估、(iii) 血液检查和 (iv) 饮食习惯评估。根据 US 评估,其中 73 人患有重度、中度或轻度肝脏脂肪变性(非酒精性脂肪肝患者),82 人无肝脏脂肪变性(健康对照组)。58名非酒精性脂肪肝患者和73名对照组完成了研究。在非酒精性脂肪肝患者中,26 人(45%)的脂肪变性严重程度有所减轻,其中 12 人(21%)的脂肪变性完全消退。健康对照组中有三人患上了非酒精性脂肪肝。与健康对照组相比,非酒精性脂肪肝患者在研究期间改善了饮食习惯,降低了体重指数和腰围。肝脏脂肪变性的缓解/消退与体重指数或肝酶的变化无关,而且在基线脂肪变性程度较轻的患者中更为常见。结论是地中海饮食建议(不包括个性化膳食计划)可有效减少/缓解非酒精性脂肪肝,尤其是轻度患者,这支持早期识别和生活方式干预。该试验已在 NCT03300661 上注册。
{"title":"Mediterranean-Oriented Dietary Intervention Is Effective to Reduce Liver Steatosis in Patients with Nonalcoholic Fatty Liver Disease: Results from an Italian Clinical Trial","authors":"Barbara Zanini,&nbsp;Federica Benini,&nbsp;Monica Marullo,&nbsp;Anna Simonetto,&nbsp;Angelo Rossi,&nbsp;Paola Cavagnoli,&nbsp;Alessia Bonalumi,&nbsp;Silvia Marconi,&nbsp;Marie Graciella Pigozzi,&nbsp;Gianni Gilioli,&nbsp;Alessandra Valerio,&nbsp;Francesco Donato,&nbsp;Maurizio Castellano,&nbsp;Chiara Ricci","doi":"10.1155/2024/8861126","DOIUrl":"10.1155/2024/8861126","url":null,"abstract":"<p><i>Background of the Study</i>. Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in western countries. Lifestyle interventions are recommended as the primary therapy for NAFLD. <i>Methodology</i>. In this clinical trial, NAFLD patients were enrolled in a 12-month dietary intervention aimed to improve their eating habits according to the Mediterranean pattern, with scheduled appointments every three months. After the exclusion of steatosis, healthy subjects were recruited and received general advice based on current Italian food-based dietary guidelines. <i>Results</i>. One hundred and fifty five subjects aged 20–59 years underwent (i) liver ultrasound (US), (ii) clinical and anthropometric evaluations, (iii) blood tests, and (iv) assessment of dietary habits. According to US evaluation, 73 of them had severe, moderate, or mild liver steatosis (NAFLD patients) and 82 had no liver steatosis (healthy controls). Fifty-eight NAFLD patients and 73 controls completed the study. Among NAFLD patients, 26 (45%) downgraded steatosis severity, 12 of which achieved complete steatosis regression (21%). Three of the healthy controls developed NAFLD. The NAFLD patients improved their dietary habits and reduced BMI and waist circumference, during the study period, more than healthy controls. Liver steatosis remission/regression was independent of changes in BMI or liver enzymes and was more frequent among patients with mild steatosis at baseline. <i>Conclusions</i>. Mediterranean dietary advices, without a personalised meal planning, were efficient in reducing/remitting NAFLD, especially among patients with mild disease, which argues in favour of early identification and lifestyle intervention. This trial is registered with NCT03300661.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Characteristics and Nomogram for Predicting Mortality in Patients with Postoperative Bloodstream Infection in Surgical Intensive Care Unit 外科重症监护病房术后血流感染患者的临床特征和死亡率预测提名图
IF 2.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2024-01-12 DOI: 10.1155/2024/9911996
Zengli Xiao, Yao Sun, Huiying Zhao, Youzhong An

Background. Bloodstream infection is amongst the leading causes of mortality for critical postoperative patients. However, data, especially from developing countries, are scary. Clinical decision-making tools for predicting postoperative bloodstream infection-related mortality are important but still lacking. Objective. To analyze the distribution of pathogens and develop a nomogram for predicting mortality in patients with postoperative bloodstream infection in the surgical intensive care unit. Methods. The clinical data, infection and pathogen-related data, and prognosis of patients with PBSI in the SICU from January 2017 to January 2022 were retrospectively collected. The distribution of pathogens and clinical characteristics of patients with PBSI were analyzed. The patients were assigned to a died group and a survived group according to their survival status. Independent predictors for mortality were identified by univariate and multivariate analyses. A nomogram for predicting PBSI-related death was developed based on these independent predictors. Calibration and decision-curve analysis were established to evaluate the nomogram. We collected postoperative patients admitted to our center from February 2022 to June 2023 as external validation sets to verify the nomogram. We also add the Brier score to further validate the model. Results. In the training set, 7128 patients admitted to the SICU after different types of surgery were collected. A total of 198 patients and 308 pathogens were finally enrolled. The mean age of patients with PBSI was 64.38 ± 16.22 (range 18–90) years, and 56.1% were male. Forty-five patients (22.7%) died in the hospital. Five independent predictors including BMI, APACHE II score, estimated glomerular filtration rate (eGFR), urine volume in the first 24 hours after surgery, and peak temperature before positive blood cultures were selected to establish the nomogram. The area under the receiver operating characteristic curve for the prediction model was 0.922. Calibration curve and decision curve analysis showed good performance of the nomogram. Seventy patients with PBSI were collected as an external validation set, and thirteen patients died in this set. The external validation set was used to validate the nomogram, and the results showed that the AUC was 0.930 which was higher than that in the training set indicating that the nomogram had a good discrimination. The brier score was 0.087 for training set and 0.050 for validation set. Conclusions. PBSI was one of the key issues that clinicians were concerned and could be assessed with a good predictive model using simple clinical factors.

背景。血流感染是危重术后病人死亡的主要原因之一。然而,相关数据,尤其是来自发展中国家的数据却非常少。预测术后血流感染相关死亡率的临床决策工具非常重要,但仍然缺乏。目标:分析病原体的分布分析病原体的分布情况,并开发一种用于预测外科重症监护病房术后血流感染患者死亡率的提名图。方法回顾性收集 2017 年 1 月至 2022 年 1 月 SICU 中 PBSI 患者的临床数据、感染和病原体相关数据以及预后。分析了 PBSI 患者的病原体分布和临床特征。根据患者的生存状况将其分为死亡组和存活组。通过单变量和多变量分析确定了死亡率的独立预测因素。根据这些独立预测因素,制定了预测 PBSI 相关死亡的提名图。校准和决策曲线分析用于评估提名图。我们收集了 2022 年 2 月至 2023 年 6 月本中心收治的术后患者作为外部验证集,以验证提名图。我们还增加了 Brier 评分来进一步验证模型。结果在训练集中,我们收集了 7128 名不同类型手术后入住 SICU 的患者。最终共有 198 名患者和 308 种病原体被纳入训练集。PBSI 患者的平均年龄为 64.38 ± 16.22(18-90 岁)岁,56.1% 为男性。45名患者(22.7%)在住院期间死亡。研究人员选择了五个独立的预测指标,包括体重指数(BMI)、APACHE II 评分、估计肾小球滤过率(eGFR)、术后 24 小时内的尿量和血培养阳性前的体温峰值,以建立提名图。预测模型的接收者操作特征曲线下面积为 0.922。校准曲线和决策曲线分析表明提名图性能良好。收集了 70 例 PBSI 患者作为外部验证集,其中 13 例患者死亡。外部验证集用于验证提名图,结果显示 AUC 为 0.930,高于训练集,表明提名图具有良好的分辨能力。训练集的布赖尔评分为 0.087,验证集为 0.050。结论PBSI 是临床医生关注的关键问题之一,可以利用简单的临床因素建立一个良好的预测模型进行评估。
{"title":"Clinical Characteristics and Nomogram for Predicting Mortality in Patients with Postoperative Bloodstream Infection in Surgical Intensive Care Unit","authors":"Zengli Xiao,&nbsp;Yao Sun,&nbsp;Huiying Zhao,&nbsp;Youzhong An","doi":"10.1155/2024/9911996","DOIUrl":"10.1155/2024/9911996","url":null,"abstract":"<p><i>Background</i>. Bloodstream infection is amongst the leading causes of mortality for critical postoperative patients. However, data, especially from developing countries, are scary. Clinical decision-making tools for predicting postoperative bloodstream infection-related mortality are important but still lacking. <i>Objective</i>. To analyze the distribution of pathogens and develop a nomogram for predicting mortality in patients with postoperative bloodstream infection in the surgical intensive care unit. <i>Methods</i>. The clinical data, infection and pathogen-related data, and prognosis of patients with PBSI in the SICU from January 2017 to January 2022 were retrospectively collected. The distribution of pathogens and clinical characteristics of patients with PBSI were analyzed. The patients were assigned to a died group and a survived group according to their survival status. Independent predictors for mortality were identified by univariate and multivariate analyses. A nomogram for predicting PBSI-related death was developed based on these independent predictors. Calibration and decision-curve analysis were established to evaluate the nomogram. We collected postoperative patients admitted to our center from February 2022 to June 2023 as external validation sets to verify the nomogram. We also add the Brier score to further validate the model. <i>Results</i>. In the training set, 7128 patients admitted to the SICU after different types of surgery were collected. A total of 198 patients and 308 pathogens were finally enrolled. The mean age of patients with PBSI was 64.38 ± 16.22 (range 18–90) years, and 56.1% were male. Forty-five patients (22.7%) died in the hospital. Five independent predictors including BMI, APACHE II score, estimated glomerular filtration rate (eGFR), urine volume in the first 24 hours after surgery, and peak temperature before positive blood cultures were selected to establish the nomogram. The area under the receiver operating characteristic curve for the prediction model was 0.922. Calibration curve and decision curve analysis showed good performance of the nomogram. Seventy patients with PBSI were collected as an external validation set, and thirteen patients died in this set. The external validation set was used to validate the nomogram, and the results showed that the AUC was 0.930 which was higher than that in the training set indicating that the nomogram had a good discrimination. The brier score was 0.087 for training set and 0.050 for validation set. <i>Conclusions</i>. PBSI was one of the key issues that clinicians were concerned and could be assessed with a good predictive model using simple clinical factors.</p>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2024 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Clinical Practice
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1