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Impact of spray drying temperatures on whey protein concentrate particle size, particle shape and related powder physical properties 喷雾干燥温度对乳清蛋白浓缩物粒度、颗粒形状及相关粉体物理性能的影响
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-18 DOI: 10.1111/1471-0307.70052
Even Gausemel, James A O'Mahony, Reidar B Schüller, Kristian H Liland, Elling-Olav Rukke, Siv B Skeie, Camilla E Jørgensen, Anne-Grethe Johansen

Background, Context, or Rationale

Whey protein concentrate (WPC) powders are widely used as ingredients in the food industry due to their high-protein content and desirable functional properties. Sub-optimal spray drying conditions can lead to poor powder flowability and/or increased cohesiveness, influencing the risk of handling challenges.

Aims

This study investigates the impact of varying spray drying temperatures and powder storage temperatures on particle size distribution, particle shape, cohesiveness and flowability of WPC80 powders.

Methods

Liquid WPC was spray-dried at three different inlet/outlet temperature combinations: lower (180°C/77°C), moderate (197°C/94°C) and higher (214°C/111°C). The powders were then stored at 20°C, 30°C or 40°C for 61 days. Flowability and cohesiveness were measured using the Carr Index and Warren–Spring cohesion, respectively. Particle morphology was analysed using dynamic image analysis (DIA), laser diffraction and scanning electron microscopy (SEM).

Major Findings

This study demonstrated that moderate and higher spray drying temperatures yielded smaller, more irregularly shaped particles with reduced flowability, compared to WPC spray-dried at lower temperatures. This was related to particle breakage, lower bulk density and increased compressibility. Lower spray drying temperatures yielded larger, more uniformly shaped particles with better flowability but higher levels of residual moisture and water activity, and more cohesive compressed powder. This study demonstrates that storage temperatures have a negligible effect on particle morphology and flowability, with only a limited influence on the cohesion of WPC powder. These findings are mainly attributed to the way storage temperature affects moisture availability, which can result in liquid bridging between WPC particles.

Scientific or Industrial Implications

Optimising spray drying temperatures can improve the physical properties of WPC80 powders, enhancing their handling behaviour by influencing particle morphology and particle–particle interactions. Future research should explore the roles of nonenzymatic browning and particle shape of WPC80 powder particles on rehydration properties.

背景、背景或原理乳清浓缩蛋白(WPC)粉末由于其高蛋白含量和理想的功能特性而广泛用作食品工业的原料。不理想的喷雾干燥条件会导致粉末流动性差和/或粘性增加,从而影响处理挑战的风险。目的研究不同喷雾干燥温度和粉末储存温度对WPC80粉末粒度分布、颗粒形状、黏结性和流动性的影响。方法采用低温度(180°C/77°C)、中温度(197°C/94°C)和高温度(214°C/111°C)三种不同的进出口温度组合喷雾干燥液体WPC。然后将粉末在20°C、30°C或40°C下保存61天。流动性和凝聚力分别采用卡尔指数和沃伦-弹簧凝聚力进行测量。采用动态图像分析(DIA)、激光衍射和扫描电镜(SEM)对颗粒形貌进行了分析。本研究表明,与低温度喷雾干燥的塑木复合材料相比,中等和较高的喷雾干燥温度产生的颗粒更小,形状更不规则,流动性更低。这与颗粒破碎、体积密度降低和压缩性增加有关。较低的喷雾干燥温度产生更大,形状更均匀的颗粒,流动性更好,但更高水平的残余水分和水活性,更有凝聚力的压缩粉末。该研究表明,储存温度对颗粒形态和流动性的影响可以忽略不计,对木塑粉的黏聚性只有有限的影响。这些发现主要归因于储存温度影响水分可用性的方式,这可能导致木塑颗粒之间的液体桥接。优化喷雾干燥温度可以改善WPC80粉末的物理性能,通过影响颗粒形态和颗粒之间的相互作用来增强其处理行为。今后的研究应进一步探讨WPC80粉末颗粒的非酶褐变和颗粒形状对复水性能的影响。
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引用次数: 0
Complexing whey protein isolate with polysaccharide to ameliorate freezing-induced damage of cream cheese 分离乳清蛋白与多糖络合改善奶油奶酪冻害的研究
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-17 DOI: 10.1111/1471-0307.70053
Celeste Sevilla, Vermont Dia, Liz Eckelkamp, Tong Wang

Rationale

Freezing dairy foods can result in textural quality loss. There is a need for food-grade antifreeze agents for long-term storage.

Aim

This study aimed to develop dairy protein–polysaccharide complexes, assess their ice recrystallisation inhibition effect and reduce freezing-induced damage by incorporating them into a commercial cream cheese spread.

Methods

Complex formation between whey protein isolate and locust bean gum (LBG) or lambda carrageenan (λCG), as well as complexes between their hydrolysates, was made and confirmed by particle size, zeta potential, surface hydrophobicity analyses and FTIR.

Major Findings

The complexes made from the unhydrolyzed biopolymers showed ~66% ice crystal size relative to a negative control for both gum types. The cream cheese spread with complex addition at 4% dry-weight basis and after repeated freeze-thaw treatment cycles showed general trends of all complex treatments containing λCG having rheological and textural properties more similar to the fresh control spread compared to the protein alone and other treatments containing LBG. The storage modulus (G’) of the treatments with complexes formed between WPI and λCG and their hydrolysates are between 200–250 KPa, similar to that of untreated control. The adhesiveness of the treatments with complexes formed between WPI and λCG and their hydrolysates, as well as the λCG gums, are also more similar to the controls (50 vs 25 g.sec), compared to the other treatments (150–250 g.sec).

Industrial Implications

When the study is further validated by applying the treatments to the proper processing steps and products evaluated under more relevant conditions, the impact of such novel antifreezing complex on dairy product quality will be better demonstrated.

原理:冷冻乳制品会导致质地的损失。需要长期储存的食品级防冻剂。本研究旨在开发乳制品蛋白-多糖复合物,评估其抑制冰重结晶的效果,并通过将其加入到商业奶油奶酪酱中来减少冷冻引起的损伤。方法分离乳清蛋白与刺槐豆胶(LBG)或λ卡拉胶(λCG)及其水解产物之间形成配合物,并通过粒径、zeta电位、表面疏水性分析和红外光谱(FTIR)进行验证。与阴性对照相比,由未水解的生物聚合物制成的复合物的冰晶大小约为66%。在干重为4%的基础上添加复合添加剂并经过反复冻融循环处理的奶油奶酪涂布,与单独添加蛋白质和其他含有LBG的处理相比,所有含有λCG的复合处理的流变学和质地特性更接近新鲜对照涂布。WPI与λCG及其水解产物形成配合物的处理的储存模量(G′)在200 ~ 250 KPa之间,与未处理的对照相似。与其他处理(150-250 g.sec)相比,WPI与λCG及其水解产物之间形成配合物的处理以及λCG胶的粘附性也更接近对照组(50 vs 25 g.sec)。当将该研究应用于适当的加工步骤并在更相关的条件下评估产品时,将进一步验证该研究,这种新型防冻复合物对乳制品质量的影响将得到更好的证明。
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引用次数: 0
Raman confocal microscopy investigation of dairy-relevant model oil in water emulsions and impact of emulsifier and temperature variation 拉曼共聚焦显微镜研究乳状液中乳状液模型油及其乳化剂和温度变化的影响
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-10 DOI: 10.1111/1471-0307.70024
Elizabeth M Nickless, Thomas Fuller, Stephen E Holroyd

Background

Emulsions are common in many food systems, including dairy food systems. Emulsifiers can have different physicochemical properties acting in different ways. Processing conditions, such as temperature and pH, can affect emulsion stability, as can storage conditions. Formulation of food emulsions to provide the required characteristics of products has some theoretical basis, but much remains empirical.

Aim(s)

Our aim was to use Raman confocal microscopy to determine the molecular arrangement of the chemical components of dairy food emulsions and understand how these arrangements, and therefore the stability and behaviour of the emulsions, change with component types and temperatures.

Methods

Raman confocal imaging was performed on multiple droplets of seven model oil-in-water emulsion systems using decolourised anhydrous milkfat and canola oil with two different emulsifiers at 4 and 24°C. The emulsions were visualised by creating integrated peak maps focussing on the Raman C–H bond region 2820–2940 cm−1. Multivariate algorithm K-means clustering was used to obtain a more detailed analysis of the Raman images, and principal components analysis was used to assess overall differences.

Major findings

Raman confocal microscopy successfully resolved and distinguished the chemical components of the model emulsions. The continuous, droplet and interfacial phases were clearly identified as chemically distinct. The interfacial region was sensitive to both fat and emulsifier type. Fat composition and concentration had a strong influence on component distribution. More complex interactions between the emulsion ingredients and/or component phases at the higher temperature were found.

Scientific or industrial implications

These results show that Raman confocal microscopy is a useful approach to the study of oil-in-water emulsions. We can now build on these data to establish a strong scientific basis for the targeted choice of emulsifiers and ingredients to resolve emulsion-related issues in the development of dairy food systems, including hybrid dairy/nondairy food emulsions.

乳剂在许多食品系统中都很常见,包括乳制品食品系统。乳化剂可以具有不同的物理化学性质,以不同的方式起作用。加工条件,如温度和pH值,会影响乳液的稳定性,储存条件也是如此。食品乳剂的配方提供产品所需的特性有一定的理论基础,但很多仍是经验。目的:我们的目的是使用拉曼共聚焦显微镜来确定乳制品乳剂中化学成分的分子排列,并了解这些排列,从而了解乳剂的稳定性和行为如何随着成分类型和温度的变化而变化。方法以脱色无水乳脂和菜籽油为原料,采用两种不同乳化剂,在4℃和24℃下对7种模型水包油乳液体系的多滴进行拉曼共聚焦成像。通过创建集中在拉曼C-H键区域2820-2940 cm−1的集成峰图来可视化乳剂。采用多元算法K-means聚类对拉曼图像进行更详细的分析,并采用主成分分析对总体差异进行评估。拉曼共聚焦显微镜成功地分辨和区分了模型乳剂的化学成分。连续相、液滴相和界面相在化学上是完全不同的。界面区域对脂肪和乳化剂类型均敏感。脂肪组成和浓度对组分分布有较大影响。在较高的温度下,乳液成分和/或组分相之间的相互作用更为复杂。这些结果表明,拉曼共聚焦显微镜是研究水包油乳剂的一种有效方法。我们现在可以在这些数据的基础上,为有针对性地选择乳化剂和成分建立强有力的科学基础,以解决乳制品食品系统开发中的乳化剂相关问题,包括混合乳制品/非乳制品食品乳化剂。
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引用次数: 0
Detection and enrichment of goat milk oligosaccharides and their effects on the metabolism of intestinal flora in the SHIME model 羊奶低聚糖的检测、富集及其对SHIME模型肠道菌群代谢的影响
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-05 DOI: 10.1111/1471-0307.70044
Yuqi Wang, Guofang Zhang, Shimei Dong, Xuewen Tang, Hongfang Cao, Ge Song, Libo Liu, Chun Li, Xiuxiu Cui

Background

Goat milk oligosaccharides (GMOS) constitute a significant bioactive component of goat dairy products. Compared with the proteins and fats in goat milk, the studies on GMOS are relatively lacking.

Aims

This study aimed to optimise isolation protocols for GMOS and explore their regulatory influence on intestinal homeostasis based on the Simulator of the Human Intestinal Microbial Ecosystem (SHIME).

Methods

Liquid chromatography-triple quadrupole mass spectrometry was employed in this study to determine the content of GMOS. A complete randomised design was conducted to optimise the key factors influencing the ultrafiltration and enzymatic processes. Subsequently, the conditions were refined for GMOS enrichment in nanofiltration based on response surface methodology.

Major findings

The optimum conditions for the nanofiltration separation of GMOS were identified as 40°C for temperature, 3.78 L/min for flow rate, 0.96 MPa for pressure and a pH of 6.88. Compared to the initial content in goat milk, the total content of the nine GMOS increased 8.87 times after nanofiltration enrichment. Notably, 3′-galactosyllactose (13.21 mg/L to 1.13 g/L) demonstrated the most significant enrichment effect. These enriched GMOS stimulated the proliferation of beneficial probiotic bacteria and enhanced the production of short-chain fatty acids, thereby contributing to the alleviation of Escherichia coli-induced gut microbiota dysbiosis in the SHIME.

Scientific implications

The findings could provide valuable insights into the separation of GMOS and promote the development of functional goat milk-derived products.

山羊奶低聚糖(GMOS)是山羊奶中重要的生物活性成分。与羊奶中的蛋白质和脂肪相比,转基因生物的研究相对缺乏。目的基于人类肠道微生物生态系统模拟器(SHIME),优化转基因生物分离方案,探讨其对肠道内稳态的调控作用。方法采用液相色谱-三重四极杆质谱联用法测定转基因mos的含量。采用完全随机设计来优化影响超滤和酶促过程的关键因素。随后,基于响应面法优化了转基因生物在纳滤中的富集条件。研究结果表明,GMOS的最佳纳滤分离条件为温度40℃、流速3.78 L/min、压力0.96 MPa、pH 6.88。与羊奶中初始含量相比,纳滤富集后9种转基因生物的总含量增加了8.87倍。其中,3′-半乳糖(13.21 mg/L ~ 1.13 g/L)的富集效果最为显著。这些富集的转基因生物刺激了有益益生菌的增殖,增强了短链脂肪酸的产生,从而有助于缓解大肠杆菌诱导的SHIME肠道微生物群失调。研究结果可为转基因生物的分离提供有价值的见解,并促进功能性羊奶产品的开发。
{"title":"Detection and enrichment of goat milk oligosaccharides and their effects on the metabolism of intestinal flora in the SHIME model","authors":"Yuqi Wang,&nbsp;Guofang Zhang,&nbsp;Shimei Dong,&nbsp;Xuewen Tang,&nbsp;Hongfang Cao,&nbsp;Ge Song,&nbsp;Libo Liu,&nbsp;Chun Li,&nbsp;Xiuxiu Cui","doi":"10.1111/1471-0307.70044","DOIUrl":"https://doi.org/10.1111/1471-0307.70044","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Goat milk oligosaccharides (GMOS) constitute a significant bioactive component of goat dairy products. Compared with the proteins and fats in goat milk, the studies on GMOS are relatively lacking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>This study aimed to optimise isolation protocols for GMOS and explore their regulatory influence on intestinal homeostasis based on the Simulator of the Human Intestinal Microbial Ecosystem (SHIME).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Liquid chromatography-triple quadrupole mass spectrometry was employed in this study to determine the content of GMOS. A complete randomised design was conducted to optimise the key factors influencing the ultrafiltration and enzymatic processes. Subsequently, the conditions were refined for GMOS enrichment in nanofiltration based on response surface methodology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Major findings</h3>\u0000 \u0000 <p>The optimum conditions for the nanofiltration separation of GMOS were identified as 40°C for temperature, 3.78 L/min for flow rate, 0.96 MPa for pressure and a pH of 6.88. Compared to the initial content in goat milk, the total content of the nine GMOS increased 8.87 times after nanofiltration enrichment. Notably, 3′-galactosyllactose (13.21 mg/L to 1.13 g/L) demonstrated the most significant enrichment effect. These enriched GMOS stimulated the proliferation of beneficial probiotic bacteria and enhanced the production of short-chain fatty acids, thereby contributing to the alleviation of <i>Escherichia coli</i>-induced gut microbiota dysbiosis in the SHIME.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific implications</h3>\u0000 \u0000 <p>The findings could provide valuable insights into the separation of GMOS and promote the development of functional goat milk-derived products.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"78 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biofilm and microstructure of small ruminant skin used in making traditional dairy products 传统乳制品中反刍小动物皮肤的生物膜和微观结构
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-05 DOI: 10.1111/1471-0307.70041
Asma Senoussi, Laura Tuminello, Haroun Chenchouni, Sara Panseri, Margherita Caccamo, Ouarda Aissaoui-Zitoun, Mohammed Nasser-Eddine Zidoune, Stefania Carpino

Background

Bouhezza is a traditional Algerian cheese that is ripened in permeable goatskin bags known as Djeld, which enable spontaneous coagulation, drainage and maturation. Despite their central role in traditional Bouhezza production, the functional mechanisms of goatskin containers are poorly understood.

Aims

This study aimed to elucidate the microstructural and microbial dynamics of goatskin used in Bouhezza cheese production, with a focus on its potential role as a semipermeable membrane and microbial reservoir.

Methods

Goatskin samples were analysed before and after drying and Lben (fermented milk) treatment using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Porosity, pore area and microbial colonisation patterns were assessed.

Major Findings

Drying significantly increased skin porosity from 5% (mean pore area: 3026 μm2) in fresh skin to 11% (mean pore area: 4250 μm2). After contact with Lben, porosity decreased to 4% in both fresh and dried skins, with mean pore areas reduced to 2703 and 3377 μm2, respectively, reflecting microbial adhesion and biofilm formation. High-resolution SEM and CLSM imaging revealed filamentous Lben structures entrapping casein and microbial cells, likely regulating moisture and air exchange during cheese maturation. Moreover, Lben contact enriched the goatskin biofilm with lactic acid bacteria and yeasts, promoting the development of unique cheese texture, flavour and biochemical profiles. These microstructural and microbial ecosystems were associated with intensified lipolysis and flavour development in skin-aged cheeses.

Scientific and industrial implications

This study demonstrated that goatskin bags serve a dual function in Bouhezza cheese production: acting both as a semipermeable maturation membrane and as a dynamic microbial ecosystem that shapes the traditional character of Bouhezza cheese. These findings offer mechanistic insights into traditional cheese maturation and support the valorisation and preservation of artisanal dairy practices. Further research on the metabolic activity of biofilms in such systems is required.

Bouhezza是一种传统的阿尔及利亚奶酪,它是在被称为Djeld的渗透性羊皮袋中成熟的,它可以自动凝固、排水和成熟。尽管它们在传统的Bouhezza生产中发挥着核心作用,但人们对山羊皮容器的功能机制知之甚少。本研究旨在阐明用于Bouhezza奶酪生产的山羊皮的微观结构和微生物动力学,重点研究其作为半透膜和微生物储层的潜在作用。方法采用扫描电镜(SEM)和共聚焦激光扫描显微镜(CLSM)对干燥和发酵乳处理前后的山羊皮样品进行分析。孔隙度、孔隙面积和微生物定植模式进行了评估。干燥使新鲜皮肤的孔隙度从5%(平均孔面积3026 μm2)增加到11%(平均孔面积4250 μm2)。与Lben接触后,新鲜和干燥皮肤的孔隙率均降至4%,平均孔隙面积分别降至2703和3377 μm2,反映了微生物的粘附和生物膜的形成。高分辨率扫描电镜和CLSM成像显示,丝状Lben结构包裹着酪蛋白和微生物细胞,可能在奶酪成熟过程中调节水分和空气交换。此外,乳酸菌和酵母丰富了山羊皮生物膜,促进了独特奶酪质地、风味和生化特征的发展。这些微观结构和微生物生态系统与皮肤老化奶酪的脂肪分解和风味发展密切相关。本研究表明,山羊皮袋在Bouhezza奶酪生产中具有双重功能:既是半透性成熟膜,又是塑造Bouhezza奶酪传统特征的动态微生物生态系统。这些发现提供了对传统奶酪成熟的机理见解,并支持了手工乳制品实践的价值和保存。需要进一步研究这种系统中生物膜的代谢活性。
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引用次数: 0
Assessment of goat milk quality based on phosphofructokinase gene mutation and interaction between phosphofructokinase and small nutrients through molecular simulation 基于磷酸果糖激酶基因突变的羊奶品质评价及磷酸果糖激酶与小营养物质相互作用的分子模拟
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-05 DOI: 10.1111/1471-0307.70047
Qin Shu, Zhuoyan Song, Yongfeng Liu

Background

Molecular biology methods are gradually applied in the detection of goat milk quality due to the limitations of traditional ways; thus, it is necessary to develop a new method to predict the contents of goat milk through genotype assay.

Aim

Phosphofructokinase (PFKM) plays a role in goat milk production, and this study aimed to build a connection between goat milk quality and PFKM genotypes.

Methods

The mutation sites of PFKM in goat milk were found using the direct sequencing method, and the interaction between PFKM and nutrients in goat milk was studied through molecular simulation technology.

Major Findings

Three mutation sites of PFKM were screened as 14237A>G, 14273C>T and 14292T>G, and the significant correlations were built between genotypes and goat milk indexes, which established a novel method for goat milk quality detection. Furthermore, the results show PFKM combining with the major nutrients (lactose, vitamin C and choline) is more stable than the primary protein. Based on molecular docking and molecular dynamics simulation data, the binding protein showed smaller RMSD, RMSF, Gyrate and SASA values, as well as more hydrogen bond numbers, indicating that the protein was more compact in structure, less dynamic, less exposed to the surface and more stable in the formation of internal hydrogen bonds. Cell culture and RT-qPCR experiments also proved that those nutrients promoted the PFKM gene expression in mammary epithelial cells.

Industrial Implications

The novel prediction method of goat milk quality through PFKM genotype presents a promising application in milk quality detection.

由于传统方法的局限性,分子生物学方法逐渐应用于羊奶质量的检测;因此,有必要建立一种新的羊奶基因型测定方法。目的探讨磷酸果糖激酶(PFKM)基因型与羊奶品质的关系,探讨PFKM基因型与羊奶品质的关系。方法采用直接测序法找到羊奶中PFKM的突变位点,并通过分子模拟技术研究羊奶中PFKM与营养物质的相互作用。筛选出PFKM的3个突变位点为14237A>;G、14273C>;T和14292T>;G,基因型与羊奶指标之间建立了显著相关关系,为羊奶品质检测建立了一种新的方法。此外,PFKM与主要营养成分(乳糖、维生素C和胆碱)结合比主要蛋白质更稳定。基于分子对接和分子动力学模拟数据,结合蛋白RMSD、RMSF、Gyrate和SASA值更小,氢键数更多,表明该蛋白结构更紧凑,动态更小,暴露表面更少,内部氢键形成更稳定。细胞培养和RT-qPCR实验也证实了这些营养物质促进了PFKM基因在乳腺上皮细胞中的表达。利用PFKM基因型预测羊奶品质的新方法在羊奶品质检测中具有广阔的应用前景。
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引用次数: 0
Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum 超免疫牛初乳体外中和肠出血性大肠杆菌O157:H7毒力活性的研究
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-07-31 DOI: 10.1111/1471-0307.70045
Girón Daniel, Marques da Silva Wanderson, Vilte Daniel, Smith Libia Yael, Cassabone María Carolina, Maldonado Verónica May, Cataldi Angel, Ibarra Cristina, Larzábal Mariano, Sacerdoti Flavia

Background, Context or Rationale

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection in humans may cause bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome (HUS). Cattle are the main reservoir of EHEC and a source of infection for humans. It has been previously demonstrated that hyperimmune bovine colostrum (HC) against virulence factors of EHEC was efficiently transferred to newborn calves through lactation. Also, it is well described that human consumption of HC can provide therapeutic effects on several gastrointestinal infections.

Aim(s)

The objectives of the present work were to produce HC using two recombinant virulence factors of EHEC as immunogen: C-terminal fragment of Intimin (C280-Int) and Shiga toxin type 2 (Stx2), and then to evaluate specific IgG levels and correlate them with the ability of HC to neutralise the cytotoxic activity of Stx2 and EHEC O157:H7 adhesion.

Methods

For this, 12 pregnant Aberdeen Angus breed cows were immunised with two doses of C280-Int and Stx2 at 40 and 20 days before calving. Colostrum was obtained within 24 h after delivery and specific IgG against Stx2 and Intimin was evaluated by ELISA. Stx2 neutralisation capacity of HC was evaluated on Vero cells. EHEC O157:H7 adherence inhibition was evaluated on human intestinal epithelial cells (HCT-8).

Major Findings

Stx2 neutraliation capacity was observed in nine of the HC obtained, and 10 colostrum could inhibit adherence of EHEC O157:H7 to HCT-8 cells. A correlation was observed between Stx2 neutralisation and adherence inhibition of EHEC with specific IgG levels thus indicating that specific IgG levels may predict the neutralisation capacity of colostrum.

Scientific or Industrial Implications

HC against EHEC virulence factors is effective in neutralising the virulence activity of EHEC O157:H7. This opens a perspective on the utilisation of HC for controlling EHEC colonisation in calves and to prevent the development HUS in humans.

肠出血性大肠杆菌(EHEC) O157:H7感染可引起出血性腹泻、出血性结肠炎和溶血性尿毒症综合征(HUS)。牛是肠出血性大肠杆菌的主要宿主和人类感染源。先前已经证明,抗肠出血性大肠杆菌毒力因子的高免疫牛初乳(HC)可以通过哺乳有效地转移到新生牛犊身上。此外,人类食用HC可以对几种胃肠道感染提供治疗效果。目的:本研究的目的是利用肠出血性大肠杆菌的两种重组毒力因子:内膜素c端片段(C280-Int)和志贺毒素2型(Stx2)作为免疫原制备HC,然后评估特异性IgG水平,并将其与HC中和Stx2和肠出血性大肠杆菌O157:H7粘附的细胞毒活性的能力联系起来。方法选用12头妊娠阿伯丁安格斯奶牛,分别在产犊前40天和20天接种C280-Int和Stx2。产后24 h内取初乳,用ELISA法检测对Stx2和Intimin的特异性IgG。在Vero细胞上评价HC对Stx2的中和能力。研究了EHEC O157:H7对人肠上皮细胞(HCT-8)粘附抑制作用。主要发现9种初乳具有Stx2中和能力,10种初乳可抑制EHEC O157:H7对HCT-8细胞的粘附。Stx2中和和肠出血性大肠杆菌粘附抑制与特异性IgG水平之间存在相关性,表明特异性IgG水平可以预测初乳的中和能力。针对肠出血性大肠杆菌毒力因子的HC可有效中和肠出血性大肠杆菌O157:H7的毒力活性。这为利用HC控制犊牛肠出血性大肠杆菌定植和防止人类发生溶血性尿毒综合征开辟了新的视角。
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引用次数: 0
New hydrodynamic (wave) method for obtaining rennet milk curd in cheese production 奶酪生产中凝乳酶的水动力(波)法制备
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-07-30 DOI: 10.1111/1471-0307.70048
Igor T Smykov

Background

The production of rennet cheeses is based on the formation of a milk gel, followed by cutting, mixing, cooking and the production of cheese grains and curd. Milk coagulation traditionally occurs in a prolonged static state, which leads to time and energy inefficiencies and necessitates additional processing.

Aim

The aim of this study was to evaluate the possibility of eliminating the cutting, mixing and cooking stages of milk gel during cheese production.

Methods

This study employed a hydrodynamic (wave) method to induce forced oscillations in milk during coagulation, with an oscillation amplitude of 8 mm and a frequency range of 0.5–2 Hz. The protein and fat content in the whey was assessed at a significance level of 0.05.

Major findings

It was found that hydrodynamic oscillations produced cheese grain and curd in milk. At low oscillation frequencies, the fat content of whey is 0.08%, well below the typical value of 0.4%. At higher frequencies, fat loss into the whey increased. The protein content in the whey remained frequency-independent and did not exceed 0.8%. The observed relationship allows for an assessment of the milk coagulation process under forced oscillations. The study demonstrates that with an appropriate selection of oscillation frequency—considering the size and shape of the container—cheese curd suitable for further processing via traditional methods can be obtained. Cheese samples produced using the hydrodynamic method exhibited similar characteristics to those produced via conventional techniques.

Scientific and industrial implications

This research enhances understanding of the interactions between destabilised casein micelles under forced oscillation conditions. The findings suggest that the hydrodynamic method holds promise for industrial cheese curd production, as it reduces processing time (by 0.5–1.5 hours) and minimises milk fat losses.

凝乳酶奶酪的生产是基于牛奶凝胶的形成,然后是切割、混合、烹饪和奶酪颗粒和凝乳的生产。传统上,牛奶凝固是在长时间的静态状态下发生的,这导致时间和能量效率低下,需要额外的处理。目的本研究的目的是评估在奶酪生产过程中消除牛奶凝胶切割、混合和烹饪阶段的可能性。方法采用水动力(波)法诱导牛奶凝固过程中的强制振荡,振荡幅度为8 mm,频率范围为0.5 ~ 2 Hz。乳清中的蛋白质和脂肪含量在0.05的显著水平上进行评估。主要发现:流体动力振荡使牛奶产生干酪粒和凝乳。在低振荡频率下,乳清的脂肪含量为0.08%,远低于0.4%的典型值。在更高的频率下,乳清中的脂肪损失增加。乳清中的蛋白质含量与频率无关,不超过0.8%。观察到的关系允许在强制振荡下对牛奶凝固过程进行评估。研究表明,考虑到容器的大小和形状,适当选择振荡频率,可以得到适合传统方法进一步加工的奶酪凝乳。用流体动力学方法生产的奶酪样品与传统技术生产的奶酪样品具有相似的特征。本研究增强了对不稳定酪蛋白胶束在强制振荡条件下相互作用的理解。研究结果表明,流体动力学方法有望用于工业奶酪凝乳生产,因为它缩短了加工时间(缩短了0.5-1.5小时),并最大限度地减少了乳脂的损失。
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引用次数: 0
Production performance predicting model for the Egyptian dairy buffalo using deep learning 基于深度学习的埃及水牛生产性能预测模型
IF 2.8 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-07-29 DOI: 10.1111/1471-0307.70046
Mohamed Abdelrahman, Sali Issa, Ahmed A. Ayad, Fatimah A. Al-Saeed, Min Gao, Montaser Elsayed Ali

Background

In smart livestock farming, machine learning (ML) has shown promising potential for enhancing precision, efficiency and productivity.

Aim(s)

This study aimed to use a smart production prediction model that can improve the management efficiency of dairy buffalo.

Methods

Deep neural networks (DNN) were applied depending on eight different recordings (protocol type, sire type, gestation length, lactation length, calving interval, parturition season, open days and dry period) as inputs to predict the calf sex, weight, lactation length, total milk and daily milk yield (DMI), respectively. Two additional traditional ML models, feedforward neural networks (FNNs) and ensemble learning (EL), were also constructed for performance comparison.

Major Findings

The results showed that DNN, FNN and EL testing accuracy for the calf sex were 86, 83 and 53.4% for lactation length; 78, 70 and 79% for total milk; yield was 78, 68 and 58.9%; and for DMY, it was 82, 70 and 71.2%, respectively.

Scientific or Industrial Implications

The present model integrates breeding, reproduction and production data to introduce an efficient model for managing buffalo production.

在智能畜牧业中,机器学习(ML)在提高精度、效率和生产力方面显示出了巨大的潜力。目的:利用智能产量预测模型,提高奶牛的管理效率。方法采用深度神经网络(Deep neural networks, DNN),根据8种不同的记录(协议类型、父系类型、妊娠期、泌乳期、产犊间隔、分娩季节、开放日和干期)作为输入,分别预测犊牛性别、体重、泌乳期、总乳量和日产奶量(DMI)。另外两种传统的机器学习模型,前馈神经网络(fnn)和集成学习(EL),也被构建用于性能比较。结果表明:DNN、FNN和EL对犊牛性别的准确率分别为86.83%和53.4%;占总牛奶的78%,70%和79%;收率分别为78、68和58.9%;DMY分别为82%、70%和71.2%。本模型整合了育种、繁殖和生产数据,引入了水牛生产管理的有效模型。
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引用次数: 0
Effect of fermentation with reconstituted milk on the texture, rheological properties, microstructure and aroma composition of milk beer 再造乳发酵对牛奶啤酒的质构、流变性能、微观结构及香气组成的影响
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-07-20 DOI: 10.1111/1471-0307.70025
Yuedong Guan, Tao Wu, Qiang He, Liang Wang

Background

Milk beer, a fermented beverage made from milk popular in China, faces limitations due to the high preservation and transportation costs associated with fresh milk.

Aim

This study aimed to explore the potential of using reconstituted milk as a fresh milk substitute for the production of milk beer.

Methods

The study analysed the physicochemical properties, water holding capacity (WHC), colour, protein particle size, texture, rheology, microstructure and aroma composition of milk beers produced from reconstituted and fresh milk.

Results

The results indicate that while there is a slight decrease in WHC (1.17%) and lightness (24.8%) and an increase in protein particle size (15.3%) in reconstituted milk beer compared with fresh milk beer, the overall quality remains comparable. The rheological properties and microstructure of both types of milk beer are similar, and sensory evaluation scores, although slightly lower for reconstituted milk beer, are still acceptable.

Interpretative conclusions

The research provides scientific support for the industrial application of reconstituted milk in milk beer production, potentially reducing costs and enhancing the market.

牛奶啤酒是一种由牛奶制成的发酵饮料,在中国很受欢迎,由于鲜牛奶的高保存和运输成本,它面临着限制。目的探讨利用重组乳作为鲜奶替代品生产牛奶啤酒的潜力。方法分析了用还原乳和鲜奶制成的牛奶啤酒的理化性质、保水能力、颜色、蛋白质粒度、质地、流变学、微观结构和香气组成。结果结果表明,与鲜奶啤酒相比,重组奶啤酒的WHC(1.17%)和亮度(24.8%)略有下降,蛋白质粒度(15.3%)略有增加,但整体品质保持相当。两种类型的牛奶啤酒的流变学性质和微观结构相似,感官评价分数,虽然稍微低的重组牛奶啤酒,仍然是可以接受的。解释性结论本研究为在牛奶啤酒生产中应用重组乳提供了科学依据,具有降低成本、扩大市场的潜力。
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引用次数: 0
期刊
International Journal of Dairy Technology
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