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Effect of casein genetic variants and glycosylation on bovine milk foaming properties 酪蛋白基因变异和糖基化对牛乳发泡特性的影响
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-11 DOI: 10.1111/1471-0307.13114
Barana Hewa Nadugala, Graham Hepworth, N. Vithanage, Charles N Pagel, Jared K. Raynes, C. Ranadheera, Amy Logan
The effects of κ‐casein (κ‐CN) and β‐casein (β‐CN) genetic variant and κ‐CN glycosylation degree (GD, low or high) on interfacial and foaming properties of bovine skim milk were investigated. No significant effect was measured for milks with different ĸ‐CN and β‐CN genetic variants. However, milks of higher GD exhibited lower surface tension, enhanced foamability and differences in secondary protein structure compared to lower GD skim milks. Glycan attachment is believed to affect surface activity and the spread and packing of protein at the foam bubble liquid–air interface, leading to differences in foaming performance.
研究了κ-酪蛋白(κ-CN)和β-酪蛋白(β-CN)遗传变异以及κ-CN糖基化程度(GD,低或高)对牛脱脂奶界面和发泡特性的影响。结果表明,不同ĸ-CN 和 β-CN 基因变异的牛奶对界面和发泡特性没有明显影响。然而,与 GD 值较低的脱脂奶相比,GD 值较高的脱脂奶表面张力较低,发泡性增强,二级蛋白结构存在差异。聚糖附着被认为会影响表面活性以及蛋白质在泡沫液气界面的扩散和堆积,从而导致发泡性能的差异。
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引用次数: 0
Effect of Streptococcus thermophilus extract on Lactiplantibacillus plantarum acidification and propagation in milk fermentation 嗜热链球菌提取物对牛奶发酵中植物乳杆菌酸化和繁殖的影响
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-06 DOI: 10.1111/1471-0307.13109
Kun Wang, Chengjie Ma

The probiotic Lactiplantibacillus plantarum could not independently ferment milk to form yoghurt. In this study, the effect of Streptococcus thermophilus extract on the acidification and propagation capabilities of L. plantarum during milk fermentation was investigated. The results showed that the addition of S. thermophilus crude cell extract significantly shortened the fermentation time and promoted the propagation of L. plantarum. Moreover, the addition of 0.2% S. thermophilus cell extract resulted in a higher acidification rate (final pH: 4.53) at 72 h of fermentation and a higher L. plantarum cell count (log 8.31 cfu/g) at 24 h of fermentation at 37°C. It was speculated that the presence of amino acids and free nucleotides in S. thermophilus lysate might have played an indispensable role during milk fermentation by L. plantarum. This study provided a novel strategy for utilising L. plantarum to ferment milk without using exogenous food ingredients.

益生菌植物乳杆菌不能独立发酵牛奶形成酸奶。本研究调查了嗜热链球菌提取物对植物乳杆菌在牛奶发酵过程中的酸化和繁殖能力的影响。结果表明,添加嗜热链球菌粗细胞提取物可显著缩短发酵时间,促进植物乳杆菌的繁殖。此外,添加 0.2% 的嗜热菌细胞提取物可提高发酵 72 小时后的酸化率(最终 pH 值:4.53),并提高 37°C 发酵 24 小时后的植物乳杆菌细胞数(对数 8.31 cfu/g)。据推测,嗜热菌裂解物中的氨基酸和游离核苷酸可能在植物乳杆菌发酵牛奶的过程中发挥了不可或缺的作用。这项研究为利用植物乳杆菌发酵牛奶提供了一种新的策略,而无需使用外源食品成分。
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引用次数: 0
Ultrasound promoted the inactivation efficacy of lactic acid against calcium-mediated biofilm formation by Pseudomonas fluorescens 超声促进乳酸对荧光假单胞菌钙介导的生物膜形成的灭活功效
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-29 DOI: 10.1111/1471-0307.13108
Hongchao Dai, Yanhe Zhang, Zhenbo Xu, Thanapop Stoteyome, Lei Yuan

This study investigated the synergistic effects of lactic acid (0.5%, 1% and 2%) and ultrasound (400 W, 20 kHz) on the inactivation of Pseudomonas fluorescens. Combined ultrasound and 2% lactic acid for 5 min reduced the planktonic cell count below 1.40 log cfu/mL. Ultrasound promoted the inactivation of P. fluorescens biofilms by lactic acid and increased the release of ATP and nucleic acids from bacterial cells by 0.09–0.22 μmol/OD and 0.043–0.113 of OD260 values, respectively. Results indicated that combined ultrasound and lactic acid can serve as a potential technique to control P. fluorescens biofilms.

本研究探讨了乳酸(0.5%、1% 和 2%)和超声波(400 W,20 kHz)对荧光假单胞菌灭活的协同效应。超声波和 2% 乳酸联合作用 5 分钟可将浮游细胞数降至 1.40 log cfu/mL 以下。超声波促进了乳酸对荧光假单胞菌生物膜的灭活,并使细菌细胞中 ATP 和核酸的释放量分别增加了 0.09-0.22 μmol/OD 和 0.043-0.113 OD260 值。结果表明,超声波与乳酸的结合可作为一种控制荧光团菌生物膜的潜在技术。
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引用次数: 0
Bio-yoghurt enriched with Spirulina-encapsulated pomegranate peel: Impact on some metabolomics, antioxidative, textural and colour properties 富含螺旋藻封装石榴皮的生物酸奶:对一些代谢组学、抗氧化、质地和颜色特性的影响
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-27 DOI: 10.1111/1471-0307.13105
Nuray Yagmur, Saliha Sahin, Lutfiye Yilmaz Ersan

As an innovative bio-dairy product, yoghurt was enriched with Spirulina-encapsulated aqueous/hydroalcoholic extracts of pomegranate peel (PP). Some metabolomics, antioxidative, textural and colour properties of bio-yoghurt samples were investigated. The samples with Spirulina (YM) and PP (YPA) had the highest lactic, citric and uric acid. The aroma profile of the samples was affected encapsulation process and storage time. The values on day 1 and day 21 were 17.84–17.04 mg/kg for acetaldehyde, 0.98–0.90 mg/kg for diacetyl and 54.42–46.65 mg/kg for acetoin. The sample with encapsulated PP aqueous extract (YEPA) had the highest TPC (2.97 mg GAE/g), and the sample with PP aqueous extract (YPA) had the highest antioxidant capacity (0.36 mg TE/g). The present study describes that bio-yoghurt enriched with Spirulina-encapsulated PP could be considered a good alternative for consumers demanding functional dairy products and a holistic approach to a circular bioeconomy.

作为一种创新的生物乳制品,酸奶中添加了螺旋藻封装的石榴皮(PP)水/水醇提取物。研究了生物酸奶样品的一些代谢组学、抗氧化、质地和颜色特性。含有螺旋藻(YM)和石榴皮(YPA)的样品中乳酸、柠檬酸和尿酸含量最高。样品的香气特征受封装过程和储存时间的影响。第 1 天和第 21 天的乙醛含量分别为 17.84-17.04 毫克/千克,双乙酰含量为 0.98-0.90 毫克/千克,乙炔含量为 54.42-46.65 毫克/千克。封装聚丙烯水提取物的样品(YEPA)具有最高的 TPC(2.97 毫克 GAE/克),聚丙烯水提取物的样品(YPA)具有最高的抗氧化能力(0.36 毫克 TE/克)。本研究表明,对于需要功能性乳制品的消费者来说,富含螺旋藻封装聚丙烯的生物酸奶是一种很好的替代品,也是实现循环生物经济的一种综合方法。
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引用次数: 0
Methicillin-resistant Staphylococcus aureus enterotoxin producers, isolated from the production chain of artisanal Coalho cheese 从手工制作的科尔霍奶酪生产链中分离出的耐甲氧西林金黄色葡萄球菌肠毒素生产者
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-17 DOI: 10.1111/1471-0307.13099
Jovilma Maria Soares de Medeiros, Felipe Veríssimo de Lima, Maria Rociene Abrantes, Carolina de Gouveia Mendes da Escóssia Pinheiro, Anderson Clayton da Silva Abreu, Bruna Lourenço Crippa, Nathália Cristina Cirone Silva, Jean Berg Alves da Silva

This study aimed to evaluate the presence of Staphylococcus aureus from the production chain of artisanal Coalho cheese, and to analyse the phenotypic profile of the strains, virulence factors and antimicrobial susceptibility profile. From the 52 samples collected, typical colonies of Staphylococcus spp. were isolated. From these positive S. aureus isolates, the genes encoding for enterotoxin production and antibiotic resistance were characterised. Additionally, agr locus typing was also performed. A significant presence of the sea enterotoxin producing gene, a higher incidence of the mecA gene and agr III group was identified. There was multiple resistance to penicillin, oxacillin, erythromycin and clindamycin in 79% of isolates. These results indicate that the artisanal Coalho cheese production process is associated with high contamination levels and significant potential for the isolated strains to produce enterotoxins. Therefore, there is cause for concern regarding the quality of the artisanal Coalho cheese produced.

本研究旨在评估手工制作的科尔霍奶酪生产链中是否存在金黄色葡萄球菌,并分析菌株的表型特征、毒力因子和抗菌药敏感性特征。从收集到的 52 份样本中,分离出了典型的葡萄球菌菌落。从这些阳性金黄色葡萄球菌分离物中,鉴定了编码产生肠毒素和抗生素耐药性的基因。此外,还进行了 agr 基因座分型。结果发现,大量存在海肠毒素产生基因,mecA 基因和 agr III 组的发生率较高。79%的分离物对青霉素、氧西林、红霉素和林可霉素具有多重耐药性。这些结果表明,手工制作的科尔霍奶酪生产过程中污染程度较高,分离出的菌株很有可能产生肠毒素。因此,手工制作的科尔霍奶酪的质量令人担忧。
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引用次数: 0
Ionic calcium variation in normal, acidic, alkaline and unstable non-acidic bovine milks and its relationship with alcohol stability 正常、酸性、碱性和不稳定非酸性牛乳中离子钙的变化及其与酒精稳定性的关系
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-16 DOI: 10.1111/1471-0307.13095
Shaeen Inaê da Luz Kunz, Aline Luiza do Nascimento, Cristina Bachmann da Silva, Ana Luiza Freitas dos Santos, Patrik Breitenbach, Rogério Ferreira, Ana Luiza Bachmann Schogor

Investigating the impact of ionic calcium (iCa) on alcohol stability and its correlation with suggested milk classification, a study analysed 379 bulk milk samples collected over 4 months. Results unveiled a significant inverse relationship between iCa concentration and alcohol test stability. Alkaline or unstable non-acidic milk exhibited no disparity in iCa content compared to normal or acidic milks. Despite fluctuations in iCa levels, seasonal variations, not milk classification, better accounted for the changes in stable milk detection in the alcohol test at the 76% setpoint. The seasonal fluctuation in iCa within bulk tank-collected milk carries implications for standardising industrial processes.

为了调查离子钙(iCa)对酒精稳定性的影响及其与建议的牛奶分类的相关性,一项研究分析了 4 个月内收集的 379 个散装牛奶样品。结果显示,离子钙浓度与酒精测试稳定性之间存在明显的反比关系。与正常或酸性牛奶相比,碱性或不稳定的非酸性牛奶的 iCa 含量没有差异。尽管 iCa 含量存在波动,但在 76% 设定点的酒精测试中,季节变化而非牛奶分类更能说明牛奶检测稳定性的变化。储罐收集的散装牛奶中 iCa 的季节性波动对工业流程标准化具有重要意义。
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引用次数: 0
The influence of different ultrafiltration set-ups on the mineral partitioning between skim milk streams 不同的超滤设置对脱脂奶中矿物质分配的影响
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-13 DOI: 10.1111/1471-0307.13097
Yuan Jiang, Valentin Rauh, Søren K. Lillevang, Leif H. Skibsted, Frank Lipnizki, Giovanni Barone, Lilia Ahrné

In this study, the partitioning of minerals in ultrafiltration (UF) streams of skim milk using cross-flow (CF-UF) and dead-end (DE-UF) was investigated using 50 kDa membrane at different temperatures (5, 10, 25, 35, 55°C). CF-UF showed higher protein retention (6.02–10.9%) compared with DE-UF (3.44–4.70%), along with more retention of calcium, with values ranging from 29.8 to 41.5 and 57.8 to 112 mM for DE-UF and CF-UF, respectively. Ionic calcium in permeates varied less for CF-UF (2.48–1.18 mM) than for DE-UF (3.11–0.98 mM). The insights provided in this study can be exploited to tailor adjustments of minerals in milk streams using UF.

本研究使用 50 kDa 膜,在不同温度(5、10、25、35、55°C)下,研究了横流(CF-UF)和死端(DE-UF)超滤脱脂牛奶中矿物质的分配情况。与 DE-UF(3.44-4.70%)相比,CF-UF 的蛋白质保留率更高(6.02-10.9%),同时钙的保留率也更高,DE-UF 和 CF-UF 的钙保留率分别为 29.8 至 41.5 毫摩尔和 57.8 至 112 毫摩尔。与 DE-UF 相比,CF-UF(2.48-1.18 mM)渗透液中离子钙的变化较小(3.11-0.98 mM)。本研究提供的启示可用于利用超滤技术对牛奶流中的矿物质进行定制调整。
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引用次数: 0
Oxidation and degradation kinetics of lactic butter supplemented with Myrtus communis essential oils 添加了香桃木精油的乳脂的氧化和降解动力学
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-11 DOI: 10.1111/1471-0307.13090
Tuba Simsek Mertoglu, Turkan Mutlu Keceli

The aim of this study was to investigate lipid oxidation/degradation kinetics of lactic butter manufactured from heat-treated and cultured cream, supplemented with Myrtus communis essential oils and stored at 4°C. Lipid oxidation followed zero-order, while vitamin degradation followed first-order reaction kinetics in fresh lactic butter. Optimising heat treatments of the cream at 70°C and 80°C for 30 s and using M. communis EOs reduced lipid oxidation and α-tocopherol degradation (P ≤ 0.05) of lactic butter samples. It was concluded that M. communis EOs is effective as antioxidant as compared to synthetic ascorbyl palmitate for improving stability of lactic butter.

本研究的目的是调查由热处理和培养的奶油制成的乳脂奶油的脂质氧化/降解动力学,这些奶油添加了香桃木精油并储存在 4°C 温度下。在新鲜乳脂奶油中,脂质氧化遵循零阶反应动力学,而维生素降解遵循一阶反应动力学。在 70°C 和 80°C 下对奶油进行 30 秒钟的优化热处理,并使用香桃木精油,可减少乳脂奶油样品的脂质氧化和 α-生育酚降解(P ≤ 0.05)。结论是,与合成抗坏血酸棕榈酸酯相比,蓖麻油是一种有效的抗氧化剂,可提高乳脂奶油的稳定性。
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引用次数: 0
A shelf life prediction method for butter based on the effects of β-carotene on colour and oxidative stability 基于β-胡萝卜素对颜色和氧化稳定性影响的黄油保质期预测方法
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-02 DOI: 10.1111/1471-0307.13078
Shujie Cheng, Shimin Wu

This study aimed to examine the impact of β-carotene, astaxanthin and phytosterol esters on the colour and oxidative stability of butter, as well as to explore a shelf life prediction method based on colour analysis. The results of correlation analysis indicated a significant linear correlation between acidity and whiteness index and yellowness index, with a linear fit coefficient (r2) exceeding 0.9796. In comparison with traditional shelf life prediction models, this method exhibited an average deviation of only 6.9% in its predicted results. The findings may contribute to the rapid evaluation of the shelf lives of commercial butter products.

本研究旨在考察β-胡萝卜素、虾青素和植物甾醇酯对黄油色泽和氧化稳定性的影响,并探索基于色泽分析的保质期预测方法。相关分析结果表明,酸度与白度指数和黄度指数之间存在显著的线性相关关系,线性拟合系数(r2)超过 0.9796。与传统的保质期预测模型相比,该方法的预测结果平均偏差仅为 6.9%。这些发现有助于快速评估商用黄油产品的保质期。
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引用次数: 0
In vitro assessment of addition of soy protein isolate on milk protein digestion and conformational behaviour 添加大豆分离蛋白对牛奶蛋白质消化和构象行为的体外评估
IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-30 DOI: 10.1111/1471-0307.13094
Qiaoya Mo, Qian Yang, Yingyi Mao, Feifei Deng, Xiaoying Xiong, Xiang Li, Wei Li

Soy protein (SP) and milk protein are nutritionally complementary proteins, while they have different postprandial kinetic manifestations. In this study, different sources of proteins (casein, whey protein and SP isolate) were compounded in different concentrations and ratios. An in vitro dynamic gastrointestinal digestive system for children was used to study the digestive behaviour. For all the concentrations assessed, a consistently high level of small peptide release was demonstrated at 2.67 g/100 mL protein concentration, while the addition of 10% SP isolate would increase the release of small peptides. Overall, the study offers novel perspectives on children's products with diverse protein sources.

大豆蛋白(SP)和牛奶蛋白是营养互补的蛋白质,但它们的餐后动力学表现不同。在这项研究中,不同来源的蛋白质(酪蛋白、乳清蛋白和SP分离物)以不同的浓度和比例复合在一起。采用体外动态儿童胃肠消化系统来研究消化行为。在评估的所有浓度中,2.67 克/100 毫升蛋白质浓度的小肽释放量一直很高,而添加 10%的分离 SP 会增加小肽的释放量。总之,这项研究为含有不同蛋白质来源的儿童产品提供了新的视角。
{"title":"In vitro assessment of addition of soy protein isolate on milk protein digestion and conformational behaviour","authors":"Qiaoya Mo,&nbsp;Qian Yang,&nbsp;Yingyi Mao,&nbsp;Feifei Deng,&nbsp;Xiaoying Xiong,&nbsp;Xiang Li,&nbsp;Wei Li","doi":"10.1111/1471-0307.13094","DOIUrl":"10.1111/1471-0307.13094","url":null,"abstract":"<p>Soy protein (SP) and milk protein are nutritionally complementary proteins, while they have different postprandial kinetic manifestations. In this study, different sources of proteins (casein, whey protein and SP isolate) were compounded in different concentrations and ratios. An <i>in vitro</i> dynamic gastrointestinal digestive system for children was used to study the digestive behaviour. For all the concentrations assessed, a consistently high level of small peptide release was demonstrated at 2.67 g/100 mL protein concentration, while the addition of 10% SP isolate would increase the release of small peptides. Overall, the study offers novel perspectives on children's products with diverse protein sources.</p>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140835932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Dairy Technology
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