Aflatoxin M1 (AFM1) is a toxic metabolite of aflatoxin B1 that frequently contaminates milk and dairy products through feed-to-milk carry-over and poses a significant public health concern, particularly for infants and young children. Owing to its carcinogenic potential, chemical stability, and association with chronic low-dose exposure, AFM1 is subject to stringent regulatory limits worldwide, necessitating reliable detection in complex dairy matrices. Conventional chromatographic and immunochemical methods remain essential for confirmatory analysis and routine screening; however, their operational limitations have stimulated interest in alternative sensing strategies. This review critically examines metal nanoparticle-based AFM1 detection approaches, including colorimetric and localized surface plasmon resonance assays, surface-enhanced Raman scattering, and electrochemical or nanozyme-assisted sensors. Emphasis is placed on toxicological relevance, regulatory considerations, matrix effects, and practical analytical performance rather than detection limits alone. The review highlights both the potential and current limitations of metal nanoparticle-based sensors as complementary tools for routine AFM1 monitoring in the dairy supply chain.
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