Pub Date : 2021-07-28DOI: 10.31487/j.ijcst.2021.01.02
Takaaki Sato, M. Nonomura, K. Yoneda, Sho Mizutani, Y. Mizutani
Although prostate-specific antigen (PSA) is a significant tumor marker for prostate cancer at present, the low specificity (approximately 33%) and so on likely lead to an overdiagnosis and patient suffering from highly invasive prostate biopsy. Complementary measures with cancer-characteristic biomarkers could improve the specificity and accuracy of diagnosis before the biopsy. Previously, “sniffer mice” were shown to be super-sensitive to differences in odors and to discriminate between odors of urine mixtures from patients with bladder cancer before and after tumor resection as well as urine odors of mice with or without experimental tumors. Here, we showed that the sniffer mice discriminate efficiently urinary odors of patients with prostate cancer using an odor plume-guided Y-maze behavioural assay. Through discrimination training in forced-odor choice, statistically significant increases in correct odor choice rates showed the super-sensitivity of sniffer mice to the olfactory cue of ppq-level urinary biomarkers for prostate cancer in 106 -fold diluted urine samples, where donor-unique odors were below the threshold. Moreover, we validated eight volatile urinary biomarkers nearly at their original relative concentrations as the prostate cancer cue even when adding a similar biomarker profile to the post-radical prostatectomy urine samples by the same behavioural score of the sniffer mice. These biomarkers and profiles could be useful for non-invasive tests for prostate and bladder cancers.
{"title":"Prostate Cancer-Induced Changes in Urinary Odors at Biomarker Concentrations of PPQ with Validation by Sniffer Mouse Behavioural Assays","authors":"Takaaki Sato, M. Nonomura, K. Yoneda, Sho Mizutani, Y. Mizutani","doi":"10.31487/j.ijcst.2021.01.02","DOIUrl":"https://doi.org/10.31487/j.ijcst.2021.01.02","url":null,"abstract":"Although prostate-specific antigen (PSA) is a significant tumor marker for prostate cancer at present, the \u0000low specificity (approximately 33%) and so on likely lead to an overdiagnosis and patient suffering from \u0000highly invasive prostate biopsy. Complementary measures with cancer-characteristic biomarkers could \u0000improve the specificity and accuracy of diagnosis before the biopsy. Previously, “sniffer mice” were shown \u0000to be super-sensitive to differences in odors and to discriminate between odors of urine mixtures from \u0000patients with bladder cancer before and after tumor resection as well as urine odors of mice with or without \u0000experimental tumors. Here, we showed that the sniffer mice discriminate efficiently urinary odors of patients \u0000with prostate cancer using an odor plume-guided Y-maze behavioural assay. Through discrimination \u0000training in forced-odor choice, statistically significant increases in correct odor choice rates showed the \u0000super-sensitivity of sniffer mice to the olfactory cue of ppq-level urinary biomarkers for prostate cancer in \u0000106\u0000-fold diluted urine samples, where donor-unique odors were below the threshold. Moreover, we validated \u0000eight volatile urinary biomarkers nearly at their original relative concentrations as the prostate cancer cue \u0000even when adding a similar biomarker profile to the post-radical prostatectomy urine samples by the same \u0000behavioural score of the sniffer mice. These biomarkers and profiles could be useful for non-invasive tests \u0000for prostate and bladder cancers.","PeriodicalId":13867,"journal":{"name":"International Journal of Cancer Science and Therapy","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86779222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.31487/j.ijcst.2020.01.02
A. Borgstein, W. Eshuis, S. Gisbertz, M. V. B. Henegouwen
Endoscopic resection (ER) is the treatment of choice for early gastric cancer (T1) without lymph node involvement. An additional gastrectomy with D2 lymphadenectomy is recommended if ER is considered as non-curative. Here, we present a case of a robot-assisted sentinel lymph node procedure performed with the use of duel-tracer, including ICG fluorescence and technetium-99, after a non-curative ESD for an early gastric tumor. Five “hot” lymph nodes were resected, one of which was positive for metastasis. A subtotal gastrectomy with D2 lymphadenectomy was performed additionally during the same procedure. This case presentation indicates the feasibility of a robot-assisted sentinel lymph node procedure in early gastric cancer.
{"title":"Robotic Sentinel Lymph Node Procedure After Endoscopic Submucosal Dissection of High Risk Early Gastric Cancer: A Case Report","authors":"A. Borgstein, W. Eshuis, S. Gisbertz, M. V. B. Henegouwen","doi":"10.31487/j.ijcst.2020.01.02","DOIUrl":"https://doi.org/10.31487/j.ijcst.2020.01.02","url":null,"abstract":"Endoscopic resection (ER) is the treatment of choice for early gastric cancer (T1) without lymph node\u0000involvement. An additional gastrectomy with D2 lymphadenectomy is recommended if ER is considered as\u0000non-curative. Here, we present a case of a robot-assisted sentinel lymph node procedure performed with the\u0000use of duel-tracer, including ICG fluorescence and technetium-99, after a non-curative ESD for an early\u0000gastric tumor. Five “hot” lymph nodes were resected, one of which was positive for metastasis. A subtotal\u0000gastrectomy with D2 lymphadenectomy was performed additionally during the same procedure. This case\u0000presentation indicates the feasibility of a robot-assisted sentinel lymph node procedure in early gastric\u0000cancer.","PeriodicalId":13867,"journal":{"name":"International Journal of Cancer Science and Therapy","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84604846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-09DOI: 10.31487/j.ijcst.2019.01.01
Tikam Chand
Having role in gene regulation and silencing, miRNAs have been implicated in development and progression of a number of diseases, including cancer. Herein, I present potential miRNAs associated with BAP1 gene identified using in-silico tools such as TargetScan and Exiqon miRNA Target Prediction. I identified fifteen highly conserved miRNA (hsa-miR-423-5p, hsa-miR-3184-5p, hsa-miR-4319, hsa-miR125b-5p, hsa-miR-125a-5p, hsa-miR-6893-3p, hsa-miR-200b-3p, hsa-miR-200c-3p, hsa-miR-505-3p.1, hsa-miR-429, hsa-miR-370-3p, hsa-miR-125a-5p, hsa-miR-141-3p, hsa-miR-200a-3p, and hsa-miR-429) associated with BAP1 gene. We also predicted the differential regulation of these twelve miRNAs in different cancer types.
{"title":"Identification of BAP1-associated MicroRNAs and Implications in Cancer Development","authors":"Tikam Chand","doi":"10.31487/j.ijcst.2019.01.01","DOIUrl":"https://doi.org/10.31487/j.ijcst.2019.01.01","url":null,"abstract":"Having role in gene regulation and silencing, miRNAs have been implicated in development and\u0000progression of a number of diseases, including cancer. Herein, I present potential miRNAs associated with\u0000BAP1 gene identified using in-silico tools such as TargetScan and Exiqon miRNA Target Prediction. I\u0000identified fifteen highly conserved miRNA (hsa-miR-423-5p, hsa-miR-3184-5p, hsa-miR-4319, hsa-miR125b-5p, hsa-miR-125a-5p, hsa-miR-6893-3p, hsa-miR-200b-3p, hsa-miR-200c-3p, hsa-miR-505-3p.1,\u0000hsa-miR-429, hsa-miR-370-3p, hsa-miR-125a-5p, hsa-miR-141-3p, hsa-miR-200a-3p, and hsa-miR-429)\u0000associated with BAP1 gene. We also predicted the differential regulation of these twelve miRNAs in\u0000different cancer types.","PeriodicalId":13867,"journal":{"name":"International Journal of Cancer Science and Therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83424449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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