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Studies on the efficacy of physical, chemical and biological aflatoxin B1 detoxification approaches in red chilli powder 红辣椒粉中黄曲霉毒素B1的物理、化学和生物解毒效果研究
Pub Date : 2009-07-09 DOI: 10.1504/IJFSNPH.2009.026920
Smita Tripathi, H. Mishra
Aflatoxin free powdered red pepper (100 g) was spiked with 100 ppb of aflatoxin B1 (AFB1) in methanol and was subjected to various physical (UV irradiation, heating, microwave); chemical (oxidation, bleaching, ammoniation, sulphitation) and biological (enzymatic) treatments. The residual AFB1 was quantified by standard methods to find the efficiency of different treatments in degrading AFB1. Amongst the selected physical methods, direct oven heating at 120°C for 12 hours produced maximum (83.32%) AFB1 degradation and UV exposure for 60 min produced 59.62% AFB1 degradation. The chemical treatments had no significant effect on AFB1 degradation in chilli powder with maximum % degradation (58.32) found only by oxidation with H2O2. The biological detoxification of 66.2% was achieved by treating spiked chilli powder with 12 IU of partially purified peroxidase (POD) isolated from garlic having specific activity of 246 mg−1 protein for 26 hours. The physical methods were more efficient over other methods in degrading AFB1, but producedsignificant nutritional losses (p ≤ 0.05). The ascorbic acid and carotenoids were most sensitive quality attribute affected by the treatments. The enzymatic treatment produced minimal quality changes in the treated sample.
不含黄曲霉毒素的粉状红辣椒(100 g)在甲醇中加入100 ppb黄曲霉毒素B1 (AFB1),并进行各种物理(紫外线照射、加热、微波)处理;化学(氧化、漂白、氨化、磺化)和生物(酶)处理。采用标准方法对AFB1残留量进行定量,以确定不同处理对AFB1的降解效率。在选择的物理方法中,120°C直接烘箱加热12小时的AFB1降解率最高(83.32%),紫外线照射60分钟的AFB1降解率为59.62%。化学处理对辣椒粉中AFB1的降解无显著影响,只有H2O2氧化处理的降解率最高,达到58.32%。用比活性为246 mg - 1的大蒜部分纯化过氧化物酶(POD)处理辣椒粉26小时,脱毒率达到66.2%。物理法降解AFB1的效率高于其他方法,但营养损失显著(p≤0.05)。抗坏血酸和类胡萝卜素是受处理影响最敏感的品质属性。酶处理在处理过的样品中产生最小的质量变化。
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引用次数: 16
Effect of anaemia on cognitive function in children 贫血对儿童认知功能的影响
Pub Date : 2009-07-09 DOI: 10.1504/IJFSNPH.2009.026916
R. Handa, Faiz Ahamad, K. Kesari, R. Prasad
Study was carried out on 150 school going children to study the relationship between iron deficiency anaemia and cognitive function. Children's were grouped into moderate anaemic, mild anaemic and non-anaemic. Haemoglobin levels were estimated by cyanmethaemoglobin method. Results indicate that 53.33% were mild anaemic, 12% moderate anaemic and 34.67% non-anaemic. Results significantly (p < 0.05) indicates that moderate anaemic children performed poor on attention, design fluency, verbal working memory N-back 2, visual working memory N-back 2, planning, dictation, mathematical calculations, intelligence, immediate verbal learning and visual and verbal memory. Moreover, mild anaemic children performed significantly poor (p < 0.05) in attention, visual working memory N-back 2, dictation, mathematical calculations, intelligence, immediate verbal learning and visual and verbal memory. We concluded that non-anaemic children performed significantly better on cognitive function tests than mild and moderate anaemic. All data are expressed as mean ± SD and were analysed by analysis of variance by using SPSS software.
研究人员对150名在校儿童进行了研究,以研究缺铁性贫血与认知功能之间的关系。儿童被分为中度贫血、轻度贫血和非贫血。用氰化血红蛋白法测定血红蛋白水平。结果轻度贫血占53.33%,中度贫血占12%,非贫血占34.67%。结果显示中度贫血儿童在注意、设计流畅性、言语工作记忆N-back 2、视觉工作记忆N-back 2、计划、听写、数学计算、智力、即时言语学习、视觉和言语记忆方面表现较差(p < 0.05)。轻度贫血儿童在注意、视觉工作记忆N-back 2、听写、数学计算、智力、即时言语学习、视觉和言语记忆方面的表现均显著差(p < 0.05)。我们得出结论,非贫血儿童在认知功能测试中的表现明显优于轻度和中度贫血儿童。所有数据均以mean±SD表示,采用SPSS软件进行方差分析。
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引用次数: 7
Dietary diversity in relation to other household food security indicators. 饮食多样性与其他家庭粮食安全指标的关系。
Pub Date : 2009-07-09 DOI: 10.1504/IJFSNPH.2009.026915
M. Faber, C. Schwabe, S. Drimie
This article describes dietary diversity in relation to other food security indicators used in a cross-sectional livelihood survey that included 499 randomly selected households within five municipalities in Greater Sekhukhune, Limpopo Province, South Africa. Indicators calculated using data collected by questionnaire included household dietary diversity score (DDS), living standards measure, months of food shortages and household food insecurity and access scale (HFIAS). Households with DDS ≤ 4 (n = 267) and DDS > 4 (n = 232) were compared using analysis of variance and χ²-test. Spearman correlation analysis was done for HFIAS and DDS. Compared to households with a DDS > 4, households with a DDS ≤ 4 had fewer assets, experienced more food shortages and had a higher HFIAS (16.0, 95% confidence interval (CI) 15.0-17.0 vs. 9.8, 95% CI 8.8-10.9). An inverse correlation between HFIAS and dietary diversity (r = −0.450; p < 0.01) was observed. Therefore, dietary diversity is a promising indicator for food security.
本文描述了一项横断面生计调查中使用的饮食多样性与其他粮食安全指标的关系,该调查包括南非林波波省大塞库库胡恩五个城市内随机选择的499户家庭。利用问卷收集的数据计算的指标包括家庭膳食多样性评分(DDS)、生活水平测量、粮食短缺月数和家庭粮食不安全和获取规模(HFIAS)。采用方差分析和χ 2检验比较DDS≤4的家庭(n = 267)和DDS≤4的家庭(n = 232)。HFIAS与DDS进行Spearman相关分析。与DDS为4的家庭相比,DDS≤4的家庭资产更少,食物短缺更多,HFIAS更高(16.0,95%置信区间(CI) 15.0-17.0比9.8,95% CI 8.8-10.9)。HFIAS与膳食多样性呈负相关(r = - 0.450;P < 0.01)。因此,膳食多样性是一个很有希望的粮食安全指标。
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引用次数: 97
Diabetes knowledge and attitudes towards prevention and health promotion: qualitative study in Sharjah, United Arab Emirates 糖尿病知识和对预防和促进健康的态度:阿拉伯联合酋长国沙迦的定性研究
Pub Date : 2009-07-09 DOI: 10.1504/IJFSNPH.2009.026921
N. Sulaiman, Aisha Hamdan, Dhafir Abdul-Latif Mahmood Al-Bedri, D. Young
This study explored the knowledge of and attitudes towards diabetes, diabetes prevention and management, and health promotion of patients attending medical centres in Sharjah, United Arab Emirates. Forty-one patients participated in an in depth interview. Common themes were drawn upon and reported. The understanding of the causation of diabetes varied, including genetics, stress and lifestyle. Most participants linked diabetes to lifestyle factors such as diet, lack of physical activity and obesity. These were, in turn, specifically linked to environmental aspects as well as the cultural framework. Cultural and contextual factors may hinder the implementation of behaviour change. With the understanding of these constraints, the necessity of developing creative strategies becomes evident. Suggestions are provided to enhance diabetes education, prevention and health promotion building upon the strengths within this unique cultural context.
这项研究探讨了在阿拉伯联合酋长国沙迦医疗中心就诊的患者对糖尿病、糖尿病预防和管理以及健康促进的知识和态度。41名患者参加了深度访谈。讨论并报告了共同的主题。对糖尿病病因的理解各不相同,包括遗传、压力和生活方式。大多数参与者将糖尿病与饮食、缺乏体育锻炼和肥胖等生活方式因素联系起来。这些问题又与环境方面以及文化框架有具体的联系。文化和环境因素可能阻碍行为改变的实施。随着对这些限制的理解,开发创造性策略的必要性变得明显。在这独特的文化背景下,提出了加强糖尿病教育、预防和健康促进的建议。
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引用次数: 13
Straining to catch up: China's food safety regime in disequilibrium 努力追赶:中国食品安全制度失衡
Pub Date : 2009-07-09 DOI: 10.1504/IJFSNPH.2009.026917
Huanan Liu, J. Hobbs, W. Kerr
The rapid pace of both economic growth and transition from a command to market economy has required a major restructuring of food safety institutions in the People's Republic of China (PRC). The institutional transformation has consistently lagged behind the changes demanded by economic transformation. The declining ability to control both information and integration into the global food economy has brought scrutiny of institutional deficiencies and spurred reforms. Incentives to invest in food safety in the private sector and food safety training of workers all along food supply chains and in the inspection service remain the major public health challenges.
经济的快速增长和从计划经济向市场经济的过渡要求中华人民共和国(PRC)对食品安全机构进行重大改革。制度转型一直落后于经济转型所要求的变革。控制信息和融入全球粮食经济的能力不断下降,引发了对制度缺陷的审视,并刺激了改革。鼓励私营部门对食品安全进行投资以及对整个食品供应链和检查服务部门的工人进行食品安全培训仍然是公共卫生方面的主要挑战。
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引用次数: 5
Differentiation of meat species by means of Polymerase Chain Reaction technique 用聚合酶链式反应技术鉴别肉类品种
Pub Date : 2008-06-20 DOI: 10.1504/IJFSNPH.2008.018855
B. Mane, V. Tanwar, P. Girish, A. Sonawane, D. Sharma
The present study was designed to investigate the efficiency of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique in differentiating meat species viz cattle, buffalo, sheep, goat, pig and chicken. Different random primers were screened with DNA extracted from meat samples of cattle, buffalo, sheep, goat, pig and chicken. Characteristic species-specific bands of (1,661 and 1,268 bp) in buffalo and (1,201 and 407 bp) in pig were obtained by amplification of primer B13. These bands were not present in any of the other meat species used in present investigation. The buffalo and pig meat was clearly differentiated from other meat species by these species-specific bands. The results show RAPD-PCR to be reliable, specific and sensitive method for differentiating meat species that may be of great value for monitoring meat substitution/adulteration by food regulatory agencies/authorities.
本研究旨在探讨随机扩增多态性dna聚合酶链反应(RAPD-PCR)技术在牛、水牛、绵羊、山羊、猪和鸡肉品种鉴别中的有效性。从牛、水牛、绵羊、山羊、猪和鸡的肉类样本中提取DNA,筛选不同的随机引物。引物B13扩增得到水牛(1,661和1,268 bp)和猪(1,201和407 bp)的特征性种特异性条带。这些条带在本研究中使用的任何其他肉类中都不存在。通过这些物种特异性条带,可以明显地将水牛和猪肉与其他肉类区分开来。结果表明,RAPD-PCR是一种可靠、特异、灵敏的肉类品种鉴别方法,对食品监管部门监测肉类替代/掺假具有重要价值。
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引用次数: 5
Influence of dietary cholesterol and stress on the metabolism of linoleic acid: Δ6-desaturase activity vs. product/precursor ratios 膳食胆固醇和应激对亚油酸代谢的影响:Δ6-desaturase活性与产物/前体比率
Pub Date : 2008-06-20 DOI: 10.1504/IJFSNPH.2008.018852
Jonathan E. Brown, M. F. Kelly
Δ6-desaturase is a membrane bound enzyme located in a pivotal position within the Essential Fatty Acid metabolic pathway. Changes in the activity of Δ6-desaturase affect the cellular provision of long chain polyunsaturated fatty acids and therefore factors that influence this enzyme are considered relevant to disease processes. This study investigated the effects of social isolation stress and cholesterol supplementation in a rodent model using two methods to estimate Δ6-desaturase activity, namely, a direct method measuring liver microsomal Δ6-desaturase activity and an indirect method measuring product?precursor ratio from tissue fatty acid compositions. Liver Δ6-desaturase activity was significantly decreased by isolation (-14%) and increased by cholesterol feeding (+9%). Liver microsomal phospholipid fatty acid composition and the associated product?precursor ratios for Δ6-desaturase did not relate to the changes observed in Δ6 desaturase activity. These results indicate that tissue fatty acid compositions should be viewed with caution in terms of their appropriateness as a surrogate for measuring Δ6-desaturase activity.
Δ6-desaturase是一种在必需脂肪酸代谢途径中处于关键位置的膜结合酶。Δ6-desaturase活性的变化影响长链多不饱和脂肪酸的细胞供应,因此影响该酶的因素被认为与疾病过程有关。本研究在啮齿动物模型中研究了社会隔离压力和胆固醇补充的影响,使用两种方法来估计Δ6-desaturase活性,即直接方法测量肝微粒体Δ6-desaturase活性和间接方法测量产物?组织脂肪酸组成的前体比例。肝脏Δ6-desaturase活性因分离而显著降低(-14%),因胆固醇饲养而显著升高(+9%)。肝微粒体磷脂脂肪酸组成及相关产物?Δ6-desaturase的前体比例与Δ6去饱和酶活性的变化无关。这些结果表明,组织脂肪酸组成应谨慎考虑其适当性作为替代测量Δ6-desaturase活性。
{"title":"Influence of dietary cholesterol and stress on the metabolism of linoleic acid: Δ6-desaturase activity vs. product/precursor ratios","authors":"Jonathan E. Brown, M. F. Kelly","doi":"10.1504/IJFSNPH.2008.018852","DOIUrl":"https://doi.org/10.1504/IJFSNPH.2008.018852","url":null,"abstract":"Δ6-desaturase is a membrane bound enzyme located in a pivotal position within the Essential Fatty Acid metabolic pathway. Changes in the activity of Δ6-desaturase affect the cellular provision of long chain polyunsaturated fatty acids and therefore factors that influence this enzyme are considered relevant to disease processes. This study investigated the effects of social isolation stress and cholesterol supplementation in a rodent model using two methods to estimate Δ6-desaturase activity, namely, a direct method measuring liver microsomal Δ6-desaturase activity and an indirect method measuring product?precursor ratio from tissue fatty acid compositions. Liver Δ6-desaturase activity was significantly decreased by isolation (-14%) and increased by cholesterol feeding (+9%). Liver microsomal phospholipid fatty acid composition and the associated product?precursor ratios for Δ6-desaturase did not relate to the changes observed in Δ6 desaturase activity. These results indicate that tissue fatty acid compositions should be viewed with caution in terms of their appropriateness as a surrogate for measuring Δ6-desaturase activity.","PeriodicalId":14113,"journal":{"name":"International Journal of Food Safety, Nutrition and Public Health","volume":"16 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2008-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79244300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Childhood obesity prevention (CHOP) programme: a conceptual framework for nutrition intervention 儿童肥胖预防规划:营养干预的概念框架
Pub Date : 2008-06-20 DOI: 10.1504/IJFSNPH.2008.018853
I. Tewfik
The prevalence and severity of people suffering from obesity has increased markedly worldwide. The WHO declared obesity a 'crisis of epidemic proportion'. This article outlines a conceptual framework for an intervention programme over three year period that can be easily adapted to prevent childhood obesity. The presented approach is based on behaviour modification model without giving foods. Family, school and children are essential counterparts to achieve meaningful improvement. Advocated by policies makers and embraced with favourite environmental factors, CHOP programme could be the conceptual framework for nutrition intervention that can be effectively integrated within the national health framework to attain public health goals.
世界范围内,肥胖人群的患病率和严重程度显著增加。世界卫生组织宣布肥胖是一种“流行病危机”。这篇文章概述了一个为期三年的干预计划的概念框架,可以很容易地用于预防儿童肥胖。提出的方法是基于不给予食物的行为改变模型。家庭、学校和儿童是实现有意义的改善必不可少的对应物。在决策者的倡导和有利的环境因素的支持下,CHOP方案可以成为营养干预的概念框架,可以有效地纳入国家卫生框架,以实现公共卫生目标。
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引用次数: 4
Metabolic Syndrome and its components among Qatari population 卡塔尔人群代谢综合征及其组成因素
Pub Date : 2008-06-20 DOI: 10.1504/IJFSNPH.2008.018858
M. Musallam, A. Bener, M. Zirie, Yousef K. Al-Gaud, Abdullah A. Al-Hamaq, M. Othman, I. Tewfik
Metabolic Syndrome (MeS) is a cluster of abnormalities including impaired glucose metabolism, central obesity, dyslipidemia and hypertension. The MeS has not been widely studied among the Arab populations, but the data available suggests that it is an increasingly common problem. Prevalence of MeS and its associated components are not available in Qatar. To estimate the prevalence of MeS and its associated components among the Qatari population and to determine its associated risk factors, a cross-sectional study was carried out among Qatari adults aged 20 years and above. Face to face interviewing using a structured questionnaire followed by laboratory tests were conducted. MeS was defined using the Adult Treatment Panel III (ATP III) criteria as well as the International Diabetes Federation criteria (IDF). The crude prevalence rate of MeS according to ATP III criteria and IDF criteria were 26.4 and 34.0%, respectively. The age-standardised prevalence of the MeS according to ATP III was 27.7% (95% CI 23.3?32.0%), (23.6% among men (95% CI 19.5?27.7%) and 32.6% among women (95% CI 28.0?37.2%)) and according to IDF criteria, the age standardised prevalence was 35.4% (95% CI 30.7?40.0%), 38.7% (95% CI 34.0?43.5%) for women and 35.8% (95% CI 31.2?40.5%) for men. Age, Body Mass Index and HbA1c were significantly associated with MeS after adjustment for a number relevant variables including; gender, marital status, educational level, exercise, smoking, etc. Prevalence of the MeS in Qatar is considerably higher than anticipated. A well-designed health education programmes to increase the awareness of the public as well as healthcare providers are highly recommended. The programme should focus on the risk factors and the health consequences of MeS.
代谢综合征(MeS)是一组包括糖代谢异常、中枢性肥胖、血脂异常和高血压在内的异常。在阿拉伯人口中,MeS尚未得到广泛研究,但现有数据表明,这是一个日益普遍的问题。在卡塔尔,MeS的流行程度及其相关组成部分尚不清楚。为了估计卡塔尔人口中MeS及其相关成分的患病率,并确定其相关风险因素,在20岁及以上的卡塔尔成年人中进行了一项横断面研究。采用结构化问卷进行面对面访谈,随后进行实验室测试。MeS的定义采用成人治疗小组III (ATP III)标准和国际糖尿病联合会(IDF)标准。根据ATP III和IDF标准,MeS的粗患病率分别为26.4%和34.0%。根据ATP III的年龄标准化患病率为27.7% (95% CI 23.3 ~ 32.0%),男性为23.6% (95% CI 19.5 ~ 27.7%),女性为32.6% (95% CI 28.0 ~ 37.2%),根据IDF标准,年龄标准化患病率为35.4% (95% CI 30.7 ~ 40.0%),女性为38.7% (95% CI 34.0 ~ 43.5%),男性为35.8% (95% CI 31.2 ~ 40.5%)。年龄、体重指数和HbA1c与MeS有显著相关,这些变量包括:性别、婚姻状况、文化程度、运动情况、吸烟情况等。卡塔尔中小企业的流行率大大高于预期。强烈建议制订精心设计的健康教育方案,以提高公众和保健提供者的认识。该方案应侧重于中小企业的风险因素和健康后果。
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引用次数: 15
Risk assessment of synthetic food colours: a case study in Hyderabad, India 合成食用色素的风险评估:印度海得拉巴的案例研究
Pub Date : 2008-06-20 DOI: 10.1504/IJFSNPH.2008.018857
Pratima Rao, R. Sudershan
The present study was an attempt to assess the risk of selected population to synthetic food colours. Children had high intakes of coloured solid (2?465 g day−1) and liquid foods (25?840 ml day−1). The study showed the predominant consumption of two colours such as tartrazine and sunset yellow mainly from sweetmeats, beverages and fast foods while colours like carmoisine, ponceau 4R and erythrosine were consumed by the intake of confectioneries, jams, jellies showing that the preference of colours is based on the type of foods consumed. The intakes of colours like tartrazine, erythrosine and sunset yellow were high among children due to ingestion of foods containing high concentrations of colours (9.45 and 4.0 mg). The study emphasised the need to evaluate the risk of the population to colours on a long-term basis.
本研究旨在评估特定人群对合成食用色素的风险。儿童摄入了大量的彩色固体(2?465 g d−1)和液体食品(25?840ml天−1)。研究表明,人们主要从甜食、饮料和快餐中摄取酒黄和日落黄等两种颜色,而从糖果、果酱和果冻中摄取焦糖素、ponceau 4R和红细胞红素等颜色,这表明人们对颜色的偏好是基于所食用食物的类型。由于摄入了含有高浓度颜色的食物(9.45毫克和4.0毫克),儿童对酒黄、红氨酸和日落黄等颜色的摄入量很高。该研究强调有必要在长期的基础上评估人群对颜色的风险。
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引用次数: 30
期刊
International Journal of Food Safety, Nutrition and Public Health
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