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Effect of the ethanolic extract of Pachygone ovata (Poir.) leaf extract on the viability of the egg and larva of Aedes aegypti (Diptera: Culicidae) in the laboratory conditions 在实验室条件下,紫皮果叶提取物乙醇提取物对埃及伊蚊卵和幼虫活力的影响
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5a.696
Haritha Kiranmai Meduri, M Subba Rao
Numerous plant species are utilised as valuable biological resources because active ingredients are inside them, which have been effectively exploited as insecticides. Pachygone ovata (Poir.), a plant species, exhibited characteristics of being predominantly non-aggressive and environmentally benign. However, its capacity to effectively regulate insect populations, namely mosquitoes, remained little explored. Dengue fever is an infectious disease caused by the dengue virus, which is predominantly transmitted by mosquito bites from the Aedes genus, with Aedes aegypti being the primary vector. This study examined the effect of an ethanolic extract derived from the leaves of Pachygone ovata on the survival rates of Aedes (Stegomyia) aegypti (Diptera: Culicidae) eggs and larvae in the laboratory of Acharya Nagarjuna University. Aedes aegypti is the arthropod vector that transmits the viruses that cause dengue fever, Zika virus, and Chikungunya fever. The vector has proved resistant to organophosphate chemicals used to combat the disease globally. Eggs and fourth-stage larvae of Aedes aegypti were exposed to three distinct concentrations of an ethanolic extract derived from the plant of P. ovata (C_1 = 0.0017 g/ml, C_2 = 0.0034 g/ml, and C_3 = 0.0051 g/ml) in a bioassay. The egg viability was greater than 70% in all three concentrations, whereas the larval mortality rate was greater than 70% in C_3.
许多植物物种作为宝贵的生物资源被利用,因为它们体内含有有效成分,这些有效成分已被有效地用作杀虫剂。紫果(Poir.)是一种植物,主要表现出非侵略性和无害环境的特征。然而,其有效调节昆虫种群(即蚊子)的能力仍然很少得到探索。登革热是一种由登革热病毒引起的传染病,主要通过伊蚊属的蚊虫叮咬传播,埃及伊蚊是主要媒介。本研究在龙树大学的实验室中研究了从紫皮果叶中提取的乙醇提取物对埃及伊蚊(隐蚊)卵和幼虫存活率的影响。埃及伊蚊是节肢动物媒介,传播导致登革热、寨卡病毒和基孔肯雅热的病毒。这种病媒已被证明对全球用于防治该疾病的有机磷化学品具有抗药性。将埃及伊蚊卵和四期幼虫分别暴露于三种不同浓度(C_1 = 0.0017 g/ml, C_2 = 0.0034 g/ml, C_3 = 0.0051 g/ml)的卵形伊蚊(Aedes aegypti)植物乙醇提取物中进行生物测定。3种浓度下虫卵存活率均大于70%,而C_3浓度下幼虫死亡率均大于70%。
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引用次数: 0
Larvicidal and mortality activities of ethanolic extract from Euphorbia indica against Aedes aegypti and Culex vishnui larvae 大戟醇提物对埃及伊蚊和vishnui库蚊幼虫的杀幼虫活性和死亡率
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.699
Aparna Tirumalasetti, Medikondu Kishore, K Surendra Babu
This study aimed to ascertain the larvicidal and mortality activities of crude ethanolic extract derived from Euphorbia indica against larvae of Aedes aegypti and Culex vishnui. A preliminary test was conducted in order to ascertain the mortality rate ranging from 10% to 90%. The concentrations for the final test encompassed a range from 0.0998 g/L to 1.498 g/L alongside a single negative control. The LC50 and LC90 values of the ethanolic extract derived from Euphorbia indica leaf were determined to be 466.90 g/L and 868.42 g/L, respectively. The larvicidal effect of Euphorbia indica leaf is attributed to a chemical component that acts as a positive allosteric modulator of the GABA pathway. The collected data was subjected to analysis using the Kruskal-Wallis statistical test. Probit analysis was employed to examine the LD50 and LD90 values. The minimal lethal dose that resulted in 100% mortality of Culex vishnui larvae was determined to be 0.2 g/100 ml (p=0.01). The probit analysis yielded LD50 and LD90 values of 0.01 g/100 ml and 0.06 g/100 ml of aquades in Culex vishnui, respectively. The larvicidal efficacy of the dose was determined to have an LD90 value of 0.06 g/100 ml of aquades, but the LD50 value was found to be 0.01 g/100 ml of aquades. The assessment of Culex vishnui treatment involved comparing the number of mortality larvae and the number of living larvae from each treatment to the control group. In conclusion, the ethanolic extract derived from the leaves of Euphorbia indica exhibits promising potential for developing mosquito larvicides.
研究了大戟粗醇提物对埃及伊蚊和vishnui库蚊幼虫的杀幼虫活性和死亡率。进行了初步测试,以确定死亡率在10%至90%之间。最终测试的浓度范围为0.0998 g/L至1.498 g/L,同时还有一个阴性对照。测定大戟叶乙醇提取物的LC50值为466.90 g/L, LC90值为868.42 g/L。大黄蜂叶的杀幼虫作用归因于一种化学成分,作为GABA途径的正变构调节剂。收集的数据使用Kruskal-Wallis统计检验进行分析。Probit分析法检测LD50和LD90值。结果表明,对vishnui库蚊幼虫100%致死的最小致死剂量为0.2 g/100 ml (p=0.01)。probit分析的LD50和LD90值分别为0.01 g/100 ml和0.06 g/100 ml。测定该剂量的杀虫效果LD90值为0.06 g/100 ml水,LD50值为0.01 g/100 ml水。对vishnui库蚊处理的评价包括比较各处理与对照组的死亡幼虫数和活幼虫数。综上所述,大戟叶乙醇提取物具有开发杀蚊幼虫剂的潜力。
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引用次数: 0
Dengue vector (Aedes aegypti) control in south Chennai using ovitraps through community participation 通过社区参与,在金奈南部使用诱卵器控制登革热病媒(埃及伊蚊)
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5a.698
B Malarvizhi, Amtuz Zehra, G Poonguzhali
Dengue fever, caused by the Dengue virus (DENV), is a mosquito-borne disease that poses a significant public health threat in many parts of the world. The primary vector for dengue transmission in Tamil Nadu is the Aedes aegypti mosquito, which exhibits anthropophilic behavior and prefers breeding in stagnant water in residential areas. This research paper focuses on evaluating the effectiveness of using ovitraps, modified with locally available materials, for Aedes aegypti control in Chennai through community participation. Five sampling locations within Chennai were selected for the surveillance study (K.K. Nagar, Nesapakkam, J.B.A.S College, Triplicane and Royapettah). Ovitraps of 5 different colors, such as black, blue, red, orange, and white were used. The egg counts varied across different locations, time periods and different coloured ovitraps. Nesapakkam area exhibited the highest egg counts, suggesting the presence of factors such as poor sanitation and drainage that promote mosquito breeding. The results of the study showed that the highest number of Aedes aegypti eggs was collected during the months of February-March, indicating increased mosquito breeding activity during the dry season. The study also found that black and blue ovitraps yielded the highest number of eggs, while red, orange, and white ovitraps had lower counts. These findings highlight the potential effectiveness of color-coded ovitraps in capturing mosquito eggs and suggest the importance of seasonal monitoring and targeted vector control efforts. To protect the public from dengue/DHF, large-scale dengue prevention can be implemented by combining this system with other routine prevention measures. This will enable individuals to integrate and learn more about the effective control of dengue vectors.
登革热由登革热病毒(DENV)引起,是一种蚊媒疾病,在世界许多地区构成重大公共卫生威胁。泰米尔纳德邦登革热传播的主要媒介是埃及伊蚊,它表现出亲人类行为,喜欢在居民区的死水中繁殖。本研究的重点是评价利用当地现有材料改良的诱卵器通过社区参与在金奈控制埃及伊蚊的有效性。在金奈选择了5个采样地点进行监测研究(K.K. Nagar、Nesapakkam、J.B.A.S College、Triplicane和Royapettah)。使用黑、蓝、红、橙、白5种不同颜色的诱卵器。卵数在不同的地点、时间和不同颜色的诱卵器上有所不同。Nesapakkam地区显示出最高的卵数,这表明存在促进蚊子繁殖的卫生条件差和排水等因素。研究结果表明,在2月至3月期间收集到的埃及伊蚊卵数量最多,这表明在旱季蚊子繁殖活动增加。研究还发现,黑色和蓝色诱卵器的产卵量最高,而红色、橙色和白色诱卵器的产卵量较低。这些发现突出了彩色诱蚊器在捕获蚊子卵方面的潜在有效性,并表明了季节性监测和有针对性的媒介控制工作的重要性。为保护公众免受登革热/登革出血热感染,可将该系统与其他常规预防措施相结合,实施大规模登革热预防。这将使个人能够整合和更多地了解登革热病媒的有效控制。
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引用次数: 0
Preliminary Phytochemical Analysis and Larvicidal Property of Chromolaena odorata (L.) against Culex quinquefasciatus 臭虫对致倦库蚊的初步植物化学分析及杀幼虫性能
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5a.694
R Ashok Kumar, C Kowsalya, A Jeyasankar
Mosquitoes are most dangerous insect in the world and they cause many diseases to birds, animals and humans. The control of mosquitoes using plant product is more effectiveness. The current study evaluates the larvicidal bioassay of Chromolaena odorata leaf extracts carried out against fourth instar larvae of Culex quinquefasciatus. The extracts were obtained from solvent such as Petroleum ether, Chloroform, and Ethanol. Extracts from C. odorata were tested for larvicidal activity at levels ranging from 0.625 ppm, 1.25 ppm, 2.5 ppm, and 5 ppm. The mortality rate of the larvae was determined 24 hours, 48 hours, and 72 hours. The calculation of LC50 value of extracts was calculated after 48hrs exposure. After a 48-hrs exposure, the LC50 value of the extracts was calculated. After 48 hrs of incubation, the C. odorata Petroleum ether extract showed 100% mortality. C. quinquefasciatus was more susceptible to plant extracts. The crude extracts of C. odorata found to better results on vector control it may be active phytochemicals present in this extract to be explored and also plant-derived compounds have significant potential in mosquito vectors control programme.
蚊子是世界上最危险的昆虫,它们会给鸟类、动物和人类带来许多疾病。使用植物产品防蚊效果较好。本研究评价了降羽草叶提取物对致倦库蚊4龄幼虫的杀虫活性。提取液由石油醚、氯仿和乙醇等溶剂提取。在0.625 ppm、1.25 ppm、2.5 ppm和5 ppm的浓度范围内测试了香桐提取物的杀虫活性。测定24小时、48小时和72小时幼虫的死亡率。暴露48h后计算提取物LC50值。暴露48h后,计算提取液LC50值。经48小时的孵育后,香桐石油醚提取物的死亡率为100%。致倦库蚊对植物提取物更敏感。经研究发现,香桐粗提物对蚊虫病媒控制效果较好,其活性植物化学成分有待开发,植物源性化合物在蚊虫病媒控制中具有重要的应用潜力。
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引用次数: 0
Susceptibility status of Cx. vishnui subgroup of mosquitoes against different Insecticides and detection of novel mutations in esterase beta gene in Cx. tritaeniorhynchus Cx的敏感性状况。蚊类对不同杀虫剂的抗性及酯酶β基因新突变的检测。tritaeniorhynchus
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i1a.662
Biplob Sarmah, Dr. Siraj Ahmed Khan, Dr. Prafulla Dutta, Kamakhya Borah
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引用次数: 0
Larvicidal activity of Murraya koenigii against Aedes Mosquito 柯氏蚊对伊蚊的杀幼虫活性
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i4a.688
Shiva Ohri, Gautampreet Singh, Amaninder Kaur
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引用次数: 0
Surveillance of Aedes mosquitoes in urban region of Udaipur district of South Rajasthan (India) to monitor potential health risk 南拉贾斯坦邦(印度)乌代普尔市城区伊蚊监测,监测潜在健康风险
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.705
Arti Prasad, Kaushal Vyas, Jaynesh Dhaked, Abeer Kadri, Kapil Chittara, Khushi Pahadiya, Utsav Jain, Varsha Meena, Nirbhay Singh Chouhan, Ashok Kumar
Globally, the mosquito borne diseases are considered as the highest public health risk. In humans, dengue fever infection is one of the most threatening arboviral disease transmitted by the Aedes mosquito. These Aedes mosquitoes can survive almost any climatic conditions and are extremely determined. As a result, in September, 2023 an entomological survey has been undertaken to estimate the risk of vectors of vector borne diseases (VBDs) in Urban Region of Udaipur city of South Rajasthan to diminish potential health risks and prevent from vector borne disease (VBD). The cross-sectional survey was initiated in indoors to collect immature stage in Hiran Magri Sector-3, University College of Science, Ashok Nagar, Madri, Reti Stand, MLSU University Campus and Gyarawas and in around 103 houses of Udaipur city. Sampling of the mosquito larvae was conducted via dipper, pipette, and sieves and it depends on the container types. Morphologically the larvae were predicted and larval indices were also measured. Repeated surveys were undertaken in selected areas of Udaipur Urban. 60 containers were positive for Aedes mosquito larvae out of 128 inspected containers. Discarded Sinks, Thermocol Boxes and Disposable Plastic Cups most favorable for breeding whereas coolers were least favorable. Every entomological indices were found to be higher than the critical level for every selected areas according to guidelines given by World Health Organization, (2003; 2011). The larval indices that are House Index (HI), Container Index (CI) and Breteau Index (BI) were found 17.48, 46.88 and 58.25 percent respectively. (80%) Aedes aegypti and (20%) Aedes albopictus were identified from collected immature stages. To control these mosquitoes (Aedes), a cautious and constant surveillance of the study areas is recommended.
在全球范围内,蚊媒疾病被认为是最大的公共卫生风险。在人类中,登革热感染是由伊蚊传播的最具威胁性的虫媒病毒性疾病之一。这些伊蚊几乎可以在任何气候条件下生存,而且非常坚定。因此,在2023年9月进行了一项昆虫学调查,以估计南拉贾斯坦邦乌代普尔市市区病媒传播疾病(VBDs)的风险,以减少潜在的健康风险并预防病媒传播疾病(VBD)。横断面调查是在室内开始的,收集了Hiran Magri 3区、大学理学院、Ashok Nagar、Madri、Reti Stand、MLSU校园和Gyarawas以及乌代浦尔市约103户房屋的未成熟阶段。蚊幼虫的取样方法主要是用斗、移液器和筛子,取样方法视容器类型而定。对幼虫进行了形态学预测,并测定了幼虫指数。在乌代普尔市区选定地区进行了反复调查,在检查的128个集装箱中,有60个集装箱伊蚊幼虫呈阳性。丢弃的水槽、温热盒和一次性塑料杯最有利于繁殖,而冷却器最不有利。根据世界卫生组织(2003年)制定的准则,发现每个选定地区的所有昆虫学指数都高于临界水平;2011)。幼虫指数分别为House指数(HI)、Container指数(CI)和Breteau指数(BI),分别为17.48%、46.88%和58.25%。未成熟蚊中埃及伊蚊占80%,白纹伊蚊占20%。为控制这些蚊子(伊蚊),建议对研究区域进行谨慎和持续的监测。
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引用次数: 0
Developmental study on sustainable control of dengue vector Aedes aegypti using green-synthesized selenium nanoparticles 绿色合成纳米硒对登革热媒介埃及伊蚊的可持续控制研究
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i6a.714
M Flory Shobana, MP Ayyappadas, R Renugadevi
In this Research, we present a green synthesis method for selenium nanoparticles (SeNPs) using Typhonium trilobatum leaves and assess their potential applications. By utilizing different parts of T. trilobatum leaves as bioreductive agents, our research focuses on environmentally sustainable, safe, and socially responsible processes for the synthesis of selenium nanoparticles. Various biophysical techniques, including UV-Vis, FTIR, FESEM, TEM, EDAX, and XRD were employed to characterize the green-synthesized selenium nanoparticles (SeNPs). The toxicity of these biosynthesized SeNPs was evaluated against the dengue vector Aedes aegypti in its larval and pupal stages, indicating promising results at low concentrations (2 to 10 ppm). The study also examined the adulticidal activity, revealing significant mortality in adult Ae. aegypti following exposure to the biosynthesized SeNPs. Moreover, we exposed Ae. aegypti eggs to varying concentrations of leaf extract and biosynthesized SeNPs for 24 hours. After the treatment period, we transferred the eggs individually into distilled water cups and assessed their hatch rates 48 hours later. Moreover, both the T. trilobatum leaf extract and SeNPs demonstrated exceptional results in preventing the hatching of Ae. aegypti eggs aged 12 to 18 hours, achieving 100% mortality. Furthermore, the study investigated the predation effectiveness of Poecilia reticulata guppy fish on Ae. aegypti larvae in I to IV instar stages. The results demonstrated that biosynthesized SeNPs displayed significant larvicidal activity and enhanced the predatory potential of the guppy fish P. reticulata. These findings suggest that T. trilobatum leaf extract synthesized SeNPs as an eco-friendly strategy for controlling mosquito vectors during their early developmental stages.
在本研究中,我们提出了一种绿色合成硒纳米粒子(SeNPs)的方法,并评估了其潜在的应用前景。通过利用三叶犀叶的不同部分作为生物还原剂,我们的研究重点是环境可持续、安全和社会负责的硒纳米颗粒合成工艺。采用UV-Vis、FTIR、FESEM、TEM、EDAX和XRD等多种生物物理技术对绿色合成的硒纳米粒子(SeNPs)进行了表征。对这些生物合成SeNPs在登革热媒介埃及伊蚊幼虫和蛹阶段的毒性进行了评估,表明在低浓度(2至10 ppm)下效果良好。该研究还检测了伊蚊的杀虫活性,揭示了伊蚊成虫的显著死亡率。暴露于生物合成SeNPs后的埃及伊蚊。此外,我们暴露了Ae。将埃及伊蚊卵置于不同浓度的叶提取物和生物合成的SeNPs中24小时。处理期结束后,我们将鸡蛋单独转移到蒸馏水杯中,并在48小时后评估其孵化率。此外,三叶藤叶提取物和SeNPs在防止伊蚊孵化方面均表现出优异的效果。埃及伊蚊卵生长12至18小时,死亡率达到100%。此外,还研究了网纹刺槐孔雀鱼对伊蚊的捕食效果。1至4龄的埃及伊蚊幼虫。结果表明,生物合成的SeNPs具有显著的杀幼虫活性,增强了孔雀鱼的捕食潜力。这些结果表明,三叶藤叶提取物可合成SeNPs作为一种生态策略,在蚊子发育早期控制其媒介。
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引用次数: 0
Insecticide resistance status in Aedes Albopictus (Skuse, 1894), (Diptera: Culicidae) populations after the first invasion of Türkiye during two years 白纹伊蚊(Skuse, 1894),(双翅目:库蚊科)种群在<s:1>基耶蚊首次入侵后2年的抗药性状况
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i6a.712
Sare Ilknur Yavasoğlu, Murat Ozturk, M Mustafa Akiner
An invasive Aedes Albopictus (Skuse, 1894) (Diptera: Culicidae) is considered one of the most crucial disease vectors. In this study, we aimed to monitor gradual changes in insecticide resistance profiles of Ae. Albopictus populations have recently invaded the Turkish territory. We collected Ae. Albopictus specimen from ten localities in the Black Sea, Marmara and Aegean regions in subsequent years. Adult Ae. Albopictus insecticide susceptibility to deltamethrin (0.05%), cyfluthrin (0.15%), etofenprox (0.5%), bendiocarb (0.1%), fenitrothion (1%) and dichloro diphenyl trikloroetan (DDT) (4%) was determined by the susceptibility test of World Health Organisation. Results showed common pyrethroid susceptibility. Bendiocarb and fenitrothion resistance was more common in the study. Interestingly, DDT resistance was still high in some populations despite the prohibition of the insecticide in Türkiye. A comparison of resistance status in two years demonstrated that populations were not under pyrethroid selection pressure in the study area. The fact that the bendiocarb and fenitrothion resistance, which was present when the populations were first established, continues after two years, demonstrates that the populations are under selection pressure in the study area. We believe that the findings in the study would help resistance management which is a major component of Ae. Albopictus control efforts.
入侵性白纹伊蚊(Skuse, 1894)(双翅目:库蚊科)被认为是最重要的病媒之一。在本研究中,我们旨在监测伊蚊对杀虫剂抗性谱的逐渐变化。白纹伊蚊种群最近入侵了土耳其领土。我们收集了Ae。随后几年在黑海、马尔马拉和爱琴海地区10个地点采集白纹伊蚊标本。成人Ae。采用世界卫生组织药敏试验测定白纹伊蚊对溴氰菊酯(0.05%)、氟氯菊酯(0.15%)、乙醚菊酯(0.5%)、苯虫威(0.1%)、杀虫硫磷(1%)和三氯二苯醚(DDT)(4%)的敏感性。结果显示对拟除虫菊酯类杀虫剂普遍敏感。本研究中以虫威和杀虫硫磷耐药较多。有趣的是,尽管在日本禁止使用DDT,但某些种群的DDT耐药性仍然很高。2年的抗性比较表明,研究区种群不存在拟除虫菊酯类杀虫剂的选择压力。种群初建时存在的对苯虫威和杀虫磷的抗性在2年后仍持续存在,说明研究区种群存在选择压力。我们相信这项研究的发现将有助于抗药性的管理,抗药性是伊蚊的主要组成部分。白纹伊蚊控制工作。
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引用次数: 0
Entomological surveillance of dengue vector in different types of breeding habitats in west District of Delhi 德里西区不同类型孳生地登革热媒介昆虫学监测
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.701
Bijendra Kumar, LR Verma, VN Bhagat, Aditi Singh
Entomological Surveillance of Dengue is one of the important aspects of vector control. The aim of this study was to find out the occurrence and abundance of breeding of Dengue Vector (Aedes) within different types of habitats at high risk areas for dengue transmission. Larval sampling was done indoor and outdoor. West Zone of MCD is divided into 29 wards; approximately 80% area of west zone falls in west district of Delhi. Various localities were covered for sampling of immature stage of Aedes. During this study, total 28,800 containers were examined from Jan 2020 to December 2021, out of 576 positive containers was reported. Plastic Containers (35.06%), Earthen Containers (26.56%), Iron Containers (26.04%), Cemented containers (7.46%), Rubber (4.51%) and Glass (0.34%). Maximum positive plastics containers (35.06%) was observed and minimum positive containers of Glass (0.34%) was observed. Therefore, to reduce larval habitats for Aedes breeding, use of water storage containers should be discouraged and another important tool as source reduction (Elimination of water stagnated containers) for the control of Dengue disease. There are two common species identified of Dengue vectors such as Aedes aegypti and Aedes albopictus which are responsible for the transmission of Dengue disease in Delhi.
登革热的昆虫学监测是病媒控制的重要方面之一。本研究的目的是了解登革热传播高风险地区不同类型生境中登革热媒介(伊蚊)的发生和孳生情况。幼虫分别在室内和室外采集。MCD西区分为29个区;西部地区大约80%的面积落在德里的西部地区。在不同地点进行伊蚊未成熟期采样。在本研究期间,从2020年1月至2021年12月,共检查了28,800个集装箱,其中报告了576个阳性集装箱。塑料容器(35.06%),陶土容器(26.56%),铁容器(26.04%),胶合容器(7.46%),橡胶(4.51%)和玻璃(0.34%)。阳性最多的是塑料容器(35.06%),阳性最少的是玻璃容器(0.34%)。因此,为减少伊蚊孳生的幼虫栖息地,应不鼓励使用储水容器,并采用另一种重要手段作为减少传染源(消除积水容器)来控制登革热。已确定的登革热病媒有两种常见物种,如埃及伊蚊和白纹伊蚊,它们在德里传播登革热。
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引用次数: 0
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International Journal of Mosquito Research
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