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Biosynthesised silver nanoparticle with Rhizophora annamalayana mangrove leaf aqueous extract and its larvicidal activity: An in-vitro study 红树根霉叶水提物生物合成纳米银及其杀幼虫活性的体外研究
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.707
Samivel Celliah, Vasugi Swamivel Rathinavelu, Krishnamoorthy Palaniyandi, Raja Krishnamoorthy
Plant extracts have been used as both reducing and capping agents, which has helped make the production of silver nanoparticles (AgNPs) more eco-friendly. Following a 30-minute treatment with aqueous leaf extracts, a change in color was evident, indicating the formation of silver nanoparticles (AgNPs). Moreover, the confirmation was achieved by analyzing data acquired from a range of analytical techniques, such as the UV-visible spectrum, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM). The larvicidal activity of these silver nanoparticles (AgNPs) was evaluated against the third instar and fourth larvae of Aedes aegypti in the present study. The results indicated that the larvae displayed a reaction that differed depending on the dosage, with AgNPs at 20 mL demonstrating the most notable effect on mortality rates. More precisely, when the concentration was 90 μg/mL, the mortality rate was 84% for 3rd instar larvae and 87.33% for 4th instar larvae. In contrast, the concentration of 12.5 μg/mL resulted in the lowest amount of activity, specifically 12.67%, on 3rd instar larvae. This phenomenon can be attributed to the likelihood of the radicals forming chelation bonds on the extensive surface area of the nanoparticles. The study demonstrates the potential use of R. annamalayana-mediated AgNPs as highly effective larvicidal agents for the development of innovative nanoparticle-based biological treatments.
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引用次数: 0
Evaluation of the susceptibility of An. gambiae s.l. to deltamethrin, chlorfenapyr and clothianidin in four agricultural areas of Benin An的敏感性评价。冈比亚对贝宁四个农业区的溴氰菊酯、杀虫腈和噻虫胺的抗性较弱
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i3a.677
D. Zoungbédji, G. G. Padonou, Alphonse Keller, Konkon ., A. Salako, C. Adoha, A. Fassinou, H. Sina, L. Baba-Mouss, M. Akogbéto
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引用次数: 0
Exploring the potential of Sphaeranthus Indicus flower extracts as natural mosquito larvicides 探讨白球花提取物作为天然杀蚊幼虫剂的潜力
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5a.690
Alex Yagoo, M. J. Milton, Jelin Vilvest
Extensive insecticide use for mosquito-borne disease control has led to resistant mosquito strains, undermining effectiveness. This study assessed the efficacy of Sphaeranthus Indicus flower extracts against Aedes Aegypti and Culex quinquefasciatus mosquitoes, major vectors of dengue, chikungunya, lymphatic filariasis and malaria etc. Extracts were evaluated for larvicidal and pupicidal effects, and LC 50 values were calculated. The hexane and methanol extracts demonstrated significant larvicidal activity with LC 50 values of 134.18 ppm and 116.54 ppm for Aedes Aegypti , and 115.96 ppm and 114.91 ppm for Culex quinquefasciatus , respectively. The methanol extract also exhibited effective pupicidal activity with LC 50 values of 172.90 ppm ( Aedes Aegypti ) and 126.98 ppm ( Culex quinquefasciatus ). Ovicidal activity was not observed. These findings suggest the potential of Sphaeranthus Indicus extracts as natural alternatives to chemical insecticides for controlling disease-carrying mosquitoes. Further research should consider safety, environmental impact, and cost-effectiveness before integrating these extracts into vector control programs, as alternatives to conventional insecticides.
为控制蚊媒疾病而大量使用杀虫剂已导致蚊子产生抗药性,削弱了防治效果。本研究评价了白球花提取物对登革热、基孔肯雅热、淋巴丝虫病和疟疾等主要病媒埃及伊蚊和致倦库蚊的防效。评价提取物的杀幼虫和杀蛾效果,并计算lc50值。正己烷和甲醇提取物对埃及伊蚊的lc50分别为134.18 ppm和116.54 ppm,对致倦库蚊的lc50分别为115.96 ppm和114.91 ppm。甲醇提取物的lc50值分别为172.90 ppm(埃及伊蚊)和126.98 ppm(致倦库蚊)。未观察到杀卵活性。这些发现表明,粉菊提取物有潜力作为化学杀虫剂的天然替代品来控制携带疾病的蚊子。在将这些提取物作为传统杀虫剂的替代品纳入病媒控制规划之前,进一步的研究应考虑安全性、环境影响和成本效益。
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引用次数: 0
Mortality and larvicidal activity of aqueous extract of Stephania japonica leaf against the larva of the mosquito Anopheles culicifacies 金银花叶水提物对库氏按蚊幼虫的杀虫活性及死亡率
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.700
Korampalli Lakshmi Krishnarao, T Raja Rajeswari
The Anopheles culicifacies mosquito is a vector for malaria found in southern India. Control of the Anopheles culicifacies mosquito as a vector needs to be carried out appropriately and in an environmentally responsive manner. The research aimed to examine the mortality and growth of Anopheles culicifacies mosquito larvae due to administration of Stephania japonica L leaf extract and to determine the effective concentration of S. japonica leaf extract in increasing mortality and reducing the growth of Anopheles culicifacies mosquito larvae. A bioassay for mosquito repellence and biocidal activity was performed. Mortality data was obtained by bio testing, which was then tested using probit analysis to determine the effectiveness of the test material. The results showed that larval mortality increased with increasing extract concentration. The extract's effectiveness against the test larvae was demonstrated by the toxicity level of the extract (LC50-48 hours) being 5.01%. Larval growth also decreased with increasing extract concentration; GI (Growth index) values ranged from 0-1, and RGI (Relative growth index) values ranged from 81% to 100%.
库氏按蚊是印度南部发现的疟疾病媒。对作为病媒的库氏按蚊的控制需要以适应环境的方式适当进行。本研究旨在研究金银花叶提取物对库氏按蚊幼虫死亡率和生长的影响,并确定金银花叶提取物对库氏按蚊幼虫死亡率和生长的有效浓度。对其驱蚊和杀虫活性进行了生物测定。通过生物测试获得死亡率数据,然后使用概率分析对其进行测试,以确定测试材料的有效性。结果表明,幼虫死亡率随提取物浓度的增加而增加。提取液(lc50 ~ 48 h)对试验幼虫的毒力水平为5.01%,表明提取液对试验幼虫的毒力有效。幼虫的生长也随提取物浓度的增加而降低;GI (Growth index)取值范围为0 ~ 1,RGI (Relative Growth index)取值范围为81% ~ 100%。
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引用次数: 0
Biological control of the mosquito: An analysis of the impediments and possibilities 蚊虫生物防治:障碍与可能性分析
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.704
Gargi Singh, Suresh Kumar, Devendra Kumar, GV Mishra
Biological control of mosquito is an eco-friendly approach for the control of some vector borne diseases. Numerous tools have been developed and applied to scale down the mosquito population, but the ultimate goal of these different strategies is only to mitigate the menace of mosquito and effectively prevent malaria like diseases. Unfortunately, most of these vectors control measurement the time limited prevention of diseases. For example, they were used as an insecticide, but insecticide resistant mosquito vectors are developed in course of time. Major efforts may be focused on generating eco-friendly alternatives. In current scenario there are many potential alternatives for eradicating and controlling the mosquito vectors biologically. Effective use of aquatic insect predators, larvivorous fishes, symbiotic bacteria, microbial agent and entopathogenic fungi for this purpose are still rather unexplored calling for further research. Biological control of mosquito vectors has been validated for several advantages over insecticides. To resolve the problem of current dependence on insecticidal-based mosquito elimination the option of biological control of this vector is ecofriendly and sustainable. The current knowledge of bio-controlling agents highlighting its significance in the field of public health with respective have been reviewed in this article along with the prospective challenges and opportunities.
蚊虫生物防制是控制某些病媒传播疾病的一种生态友好的方法。已经开发并应用了许多工具来减少蚊子的数量,但这些不同策略的最终目标只是减轻蚊子的威胁并有效预防类似疟疾的疾病。不幸的是,这些病媒控制措施大多对疾病的预防时间有限。例如,它们被用作杀虫剂,但随着时间的推移,蚊子媒介会产生抗药性。主要的努力可能集中在生产环保的替代品上。在目前的情况下,有许多潜在的替代方法可以从生物学上根除和控制蚊媒。有效利用水生昆虫捕食者、食饵鱼类、共生细菌、微生物制剂和内生真菌来达到这一目的还有待进一步研究。生物防治蚊虫媒介已被证实具有优于杀虫剂的若干优点。为了解决目前依赖杀虫剂灭蚊的问题,生物防治是生态友好和可持续的选择。本文综述了生物防治剂的研究现状,重点介绍了生物防治剂在公共卫生领域的重要意义,以及生物防治剂面临的挑战和机遇。
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引用次数: 0
Mosquito repellent activities of citrus fruit peels 柑桔果皮驱蚊活性研究
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5b.702
Jeevarathinam Antony, Asifa Vijayakumar, Arthi Murugesan, Rajamannar Veeramanoharan, Muniaraj Mayilsamy
Mosquitoes are accountable for the spread of numerous diseases like malaria, Dengue, Zika, Yellow fever, Chikungunya etc. among human beings. The best way to limit mosquito contact is to use personal protection and insect repellents. The majority of chemical repellents however, is unhealthy for people and can have negative effects on their skin in addition to other health-related issues. As a remedy, mosquito repellents from natural sources may not create any such kind of harm and could be a better alternative to synthetic repellents. Hence, natural repellents are preferable to chemical ones. Therefore, this study was designed to utilize an olfactometer to examine mosquito vector behaviour in response to fresh citrus fruit peel extracts. The statistical analysis showed that there were significant differences in mosquito repellency of three citrus peel extracts against the four mosquito species tested (F = 3.1668, df- 3, p
蚊子是疟疾、登革热、寨卡病毒、黄热病、基孔肯雅热等多种疾病在人类之间传播的罪魁祸首。限制蚊子接触的最好方法是使用个人防护和驱蚊剂。然而,大多数化学驱蚊剂对人来说是不健康的,除了其他与健康有关的问题外,还会对皮肤产生负面影响。作为一种补救措施,天然来源的驱蚊剂可能不会造成任何此类伤害,可能是合成驱蚊剂的更好替代品。因此,天然驱蚊剂比化学驱蚊剂更可取。因此,本研究旨在利用嗅觉仪检测蚊虫对新鲜柑橘果皮提取物的反应。统计分析表明,3种柑桔皮提取物对4种蚊虫的驱蚊效果差异有统计学意义(F = 3.1668, df- 3, p
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引用次数: 0
Natural mosquito control: Vitex negundo and Argemone mexicana as larvicidal agents against Anopheles subpictus 自然防蚊:用白荆和墨西哥银蠓杀亚按蚊
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i4a.681
R. Maheshwari, M. Madhavi, L. Mahesh, L. Mayookha
Mosquitoes are notorious vectors of severe diseases, posing significant threats to human health. Although pesticides are commonly used for mosquito control, their indiscriminate application can harm the environment and non-target organisms. Consequently, alternative strategies, such as plant-based mosquitocides, are gaining interest. This study evaluated the larvicidal efficacy of methanolic leaf extracts from Vitex negundo and Argemone mexicana against the fourth instar larvae of Anopheles subpictus. The extracts were analyzed for phytochemical compositions. Larvicidal bioassay showed concentration-dependent activity of both extracts against An. subpictus. V. negundo extract exhibited 86.36% mortality at 400 ppm, while A. mexicana extract showed 83.33% mortality at the same concentration. Probit analysis determined LC 50 and LC 90 values for V. negundo as 217.06 ppm and 404.32 ppm, respectively, and for A. mexicana as 224.45 ppm and 420.34 ppm, respectively. These findings suggest the potential of V. negundo and A. mexicana as natural larvicidal agents against An. subpictus .
蚊子是严重疾病的臭名昭著的传播媒介,对人类健康构成重大威胁。虽然杀虫剂通常用于控制蚊子,但它们的滥用会损害环境和非目标生物。因此,植物基杀虫剂等替代策略正引起人们的兴趣。本研究评价了牡荆叶甲醇提取物和墨西哥银杏叶甲醇提取物对亚按蚊4龄幼虫的杀灭效果。对提取物进行植物化学成分分析。两种提取物的杀虫活性均呈浓度依赖性。subpictus。在400 ppm浓度下,黑荆芥提取物的死亡率为86.36%,而墨荆芥提取物的死亡率为83.33%。Probit分析结果表明,黑稻的LC 50和LC 90分别为217.06 ppm和404.32 ppm,墨西哥稻的LC 50和LC 90分别为224.45 ppm和420.34 ppm。这些发现提示黑弧菌和墨西哥弧菌具有作为天然杀幼虫剂的潜力。subpictus。
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引用次数: 0
Anopheles species (Diptera: Culicidae) in kodok locality, upper Nile state, Republic of South Sudan 南苏丹共和国上尼罗州kodok地区按蚊种(双翅目:库蚊科)
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i5a.691
Venancio A. Okic, N. Bashir, Y. O. Assad
Anopheline mosquitoes (Diptera: Culicidae) consist of a large number of species. The distribution (Mapping) of malaria vectors is important for strategic planning and control strategies. Malaria risk within communities is usually associated with exposure to its vectors inside households (HHs) and necessarily with the existing larval habitats near the HHs. Studies were conducted from April 2020 to March 2022 in 4 areas/Hai of the Kodok locality (KL), Upper Nile State (UNS), Republic of South Sudan (RSS), to determine the indoor resting density and its seasonal changes, in addition to the number of Anopheles and other mosquitoes in a given room by adopting the pyrethrum spray sheet (PSS) collection technique for adults (80 HHs) and the dipping method for larval collection from 8 potential breeding sites. A total of 63,644 adults were collected and identified as follows: A. gambiae S.S . (68%), A. arabiensis (26%) and other mosquitoes (6%). The density / HH in the rainy-season (April -Oct.) varied between HHs; viz . A. gambiae s.s. (52-67) and A. arabiensis (20-28). In the dry -season (Nov. to March) the numbers were 2-12 and 0-5, respectively. A total of 7,704 larvae was collected, of which A. gambiae formed 88% and the other mosquitoes formed 12%, in the inspected breeding sites (384 visits). It is concluded that several Anopheles species are present in KL. A. gambiae is the dominant species during both dry -and wet –seasons. Densities were higher in the wet-than the dry-season. This information must be seriously considered during the vector control (VC) programs.
按蚊(双翅目:库蚊科)种类繁多。疟疾病媒的分布(绘图)对战略规划和控制战略很重要。社区内的疟疾风险通常与家庭内接触其病媒有关,而且必然与家庭附近现有的幼虫栖息地有关。2020年4月至2022年3月,在南苏丹共和国(RSS)上尼罗州(UNS) Kodok地区(KL)的4个地区/Hai进行了研究,采用除虫菊喷雾片(PSS)采集成虫(80只HHs)和浸渍法采集幼虫,确定室内静息密度及其季节变化,以及特定房间内按蚊和其他蚊子的数量。共收集成虫63,644只,鉴定结果如下:(68%),阿拉伯按蚊(26%)和其他蚊(6%)。雨季(4 - 10月)不同时段的密度/ HH差异较大;即。gambiae s.s s(52-67)和arabiensis(20-28)。在旱季(11月至3月),这一数字分别为2-12和0-5。在384次调查的孳生场所共捕获蚊幼虫7704只,其中冈比亚伊蚊占88%,其他蚊占12%。结果表明,甘比亚按蚊在干湿季节均为优势种。湿季密度高于旱季。在病媒控制(VC)程序中必须认真考虑这些信息。
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引用次数: 0
A systematic review on concept of Malaria (Visham Jwara) and its management by Godanti Bhasma Godanti Bhasma对疟疾概念及其管理的系统回顾
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i6a.713
Dr. Munesh Yadav
Developing nations are more susceptible to illnesses carried by vectors than are developed nations. Malaria is a severe issue in Africa; it is responsible for 20% of all children fatalities and results in a kid dying from the disease every 30 seconds. Malaria incidence and mortality rates are both rising quickly. With the development of drug-resistant strains of Plasmodium Vivax and Plasmodium Falciparum, as well as the vectors' tolerance to chemical pesticides, the malaria issue has taken on new dimensions. These are the major challenges of controlling malaria, hence developing new methods of doing so is urgently necessary. Vishama Jwara, or irregular or intermittent fever, has two main sorts of causes: Agantuja (External forces) and Svabhavaja Hetu (Internal reasons). Apart from these different Jwara Nidanas, the onset of Vishama Jwara (irregular or intermittent fever) is also influenced by Samanya Jwara Nidana (general aetiology of fever) as described in Sushruta Samhita and Sannipatika Jwara Nidana (aetiology of fever caused by Tridosha) as described in Charaka Samhita. The conceptual study and the observational investigation are the two sections of the current study. The length of the infection and the strain of Plasmodium determine how severe it is.
发展中国家比发达国家更容易感染病媒传播的疾病。疟疾是非洲的一个严重问题;在所有儿童死亡人数中,有20%是由该病造成的,每30秒就有一名儿童死于该病。疟疾发病率和死亡率都在迅速上升。随着间日疟原虫和恶性疟原虫耐药菌株的发展,以及媒介对化学农药的耐受性,疟疾问题呈现出新的维度。这些都是控制疟疾的主要挑战,因此迫切需要开发新的方法。Vishama Jwara,或不规则或间歇性发烧,有两种主要原因:Agantuja(外部力量)和Svabhavaja Hetu(内部原因)。除了这些不同的Jwara Nidanas之外,Vishama Jwara(不规则或间歇性发烧)的发病也受到《Sushruta Samhita》和《Charaka Samhita》中描述的Samanya Jwara Nidana(发烧的一般病因学)和Sannipatika Jwara Nidana(由热病引起的发烧的病因学)的影响。概念研究和观察调查是目前研究的两个部分。感染的持续时间和疟原虫的种类决定了感染的严重程度。
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引用次数: 0
Identification of a plant derivative (Hibiscus cannabinus) for mosquito (Anopheles darlingi) control using in silico protein-protein docking techniques 用硅蛋白-蛋白对接技术鉴定一种控制达林按蚊的植物衍生物大麻芙蓉
Pub Date : 2023-01-01 DOI: 10.22271/23487941.2023.v10.i4a.683
N. G, Prabha V
Hibiscus cannabinus, often known as kenaf, is contain significant amount of bioactive compounds and acts as a natural antioxidant. The aim of this in silico investigation is to find out how the flower extract, Sulfite oxidase inhibits the mosquito ( Anopheles darlingi ) protein, pro-resilin, using 3D automated drug docking studies. In this methodology, we perform primary analysis on the amino acid sequences of pro-resilin and sulphite oxidase using NCBI GenPept and HDock server for performing drug docking analysis. The overall results clearly elucidate that the intramolecular dynamic interaction between Sulfite oxidase and the motif regions of Pro-resilin is very effective. This results in the downregulation of the expression of Pro-resilin which has been shown in 3D form. At present, research on mosquito control is of prime focus at global level. Our in silico research investigation is a safe initiative to control mosquitoes using flower extract as it is devoid of side effects.
木槿大麻,通常被称为红麻,含有大量的生物活性化合物,是一种天然的抗氧化剂。这项计算机研究的目的是利用3D自动药物对接研究,找出花提取物亚硫酸盐氧化酶如何抑制蚊子(按蚊)蛋白质,前弹性蛋白。本方法采用NCBI GenPept和HDock服务器对原弹性蛋白和亚硫酸盐氧化酶的氨基酸序列进行初步分析,进行药物对接分析。综上所述,亚硫酸盐氧化酶与Pro-resilin基序区域之间的分子内动态相互作用是非常有效的。这导致Pro-resilin表达的下调,这已经以3D形式显示出来。目前,蚊虫控制研究是全球关注的焦点。我们的计算机研究调查是一项安全的倡议,使用花提取物控制蚊子,因为它没有副作用。
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引用次数: 1
期刊
International Journal of Mosquito Research
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