Pub Date : 2020-04-30DOI: 10.30704/http-www-jivs-net.700692
E. Karakurt, M. Kuru, S. Dağ, E. Beytut, H. Nuhoğlu, M. Demir, C. Kaçar, Özhan Karataș, A. Yıldız
In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin & Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells.
{"title":"Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors","authors":"E. Karakurt, M. Kuru, S. Dağ, E. Beytut, H. Nuhoğlu, M. Demir, C. Kaçar, Özhan Karataș, A. Yıldız","doi":"10.30704/http-www-jivs-net.700692","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.700692","url":null,"abstract":"In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin & Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123567180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.30704/http-www-jivs-net.601639
Gülşah Uyunmaz Sakli, O. Bulut, M. Hasöksüz, H. H. Hadimli
This study has investigated bovine coronavirus (BCoV) and bovine rotavirus (BRV), which are among the most important causes of diarrhea in calves leading to financial losses in Turkey and all over the world BCoV and BRV were detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), which is one of the most reliable method of diagnosis, The results obtained by RT-PCR were compared to the sensitivity of the commercial Rota-Corona Rapid Test Kits used by clinical veterinarians in fields. In this study, 96 fecal samples were examined from diarrheic calves in cattle farms in the cities of Konya and Afyon for BRV and BCoV firstly by BoviD-5 Ag rapid test kit, and then we applied the RT-PCR test. A comparison of the rapid test kit with the RT-PCR in terms of sensitivity and specificity revealed the 83% sensitivity and 100% specificity of the BRV and 7.6% sensitivity and 100% specificity of BCoV. In conclusion the practical and rapid diagnosis of the disease using of Rapid Diagnosis kit used by the clinician veterinarians may be useful, but the results must be interpreted with caution since the sensitivity of the test decreases due to the reduction in the number of viruses in the later stages of the infection. .
{"title":"Investigation of bovine coronavirus and bovine rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf feces","authors":"Gülşah Uyunmaz Sakli, O. Bulut, M. Hasöksüz, H. H. Hadimli","doi":"10.30704/http-www-jivs-net.601639","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.601639","url":null,"abstract":"This study has investigated bovine coronavirus (BCoV) and bovine rotavirus (BRV), which are among the most important causes of diarrhea in calves leading to financial losses in Turkey and all over the world BCoV and BRV were detected by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), which is one of the most reliable method of diagnosis, The results obtained by RT-PCR were compared to the sensitivity of the commercial Rota-Corona Rapid Test Kits used by clinical veterinarians in fields. In this study, 96 fecal samples were examined from diarrheic calves in cattle farms in the cities of Konya and Afyon for BRV and BCoV firstly by BoviD-5 Ag rapid test kit, and then we applied the RT-PCR test. A comparison of the rapid test kit with the RT-PCR in terms of sensitivity and specificity revealed the 83% sensitivity and 100% specificity of the BRV and 7.6% sensitivity and 100% specificity of BCoV. In conclusion the practical and rapid diagnosis of the disease using of Rapid Diagnosis kit used by the clinician veterinarians may be useful, but the results must be interpreted with caution since the sensitivity of the test decreases due to the reduction in the number of viruses in the later stages of the infection. .","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131556938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.30704/HTTP-WWW-JIVS-NET.565605
H. Atalay
In this study, the effect of serum β-hydroxybutyric acid and calcium concentrations, which are the parameters used in the diagnosis of ketosis and hypocalcemia in lactation period, on left displaced abomasum (LDA) has been investigated. The lactation period covering the 3 weeks before and after parturition, known as the transition period, is highly important for high yield dairy cows (Holstein Friesian cattle). Hormonal and metabolic changes occur in this period. The energy requirement, which increases in direct proportion with the milk yield at the beginning of lactation, cannot be met with insufficient dry matter consumption, however, it is compensated with the mobilization of body fat. Ketosis and fatty liver are nutritional diseases that are observed in animals with high milk yield resulting from the disturbances in energy metabolism. Hypocalcemia and ketosis are the most important risk factors in the development of left displaced abomasum (LDA) in high-yield milk cows. Hypocalcemia and ketosis are also the most important nutritional diseases in the transition period. In this study, 17 Holstein Friesian cattle were used in group 1 that were diagnosed with left displaced abomasum after hearing the ‘‘ping’’ sound, and 17 healthy, randomly selected Holstein Friesian cattle were used in group 2. Blood analyses (BHBA and Ca) were performed in group 1 and 2 after parturition.
{"title":"The effect of serum β-hydroxybutyric acid and calcium levels on left displaced abomasum in Holstein cows on transition period","authors":"H. Atalay","doi":"10.30704/HTTP-WWW-JIVS-NET.565605","DOIUrl":"https://doi.org/10.30704/HTTP-WWW-JIVS-NET.565605","url":null,"abstract":"In this study, the effect of serum β-hydroxybutyric acid and calcium concentrations, which are the parameters used in the diagnosis of ketosis and hypocalcemia in lactation period, on left displaced abomasum (LDA) has been investigated. The lactation period covering the 3 weeks before and after parturition, known as the transition period, is highly important for high yield dairy cows (Holstein Friesian cattle). Hormonal and metabolic changes occur in this period. The energy requirement, which increases in direct proportion with the milk yield at the beginning of lactation, cannot be met with insufficient dry matter consumption, however, it is compensated with the mobilization of body fat. Ketosis and fatty liver are nutritional diseases that are observed in animals with high milk yield resulting from the disturbances in energy metabolism. Hypocalcemia and ketosis are the most important risk factors in the development of left displaced abomasum (LDA) in high-yield milk cows. Hypocalcemia and ketosis are also the most important nutritional diseases in the transition period. In this study, 17 Holstein Friesian cattle were used in group 1 that were diagnosed with left displaced abomasum after hearing the ‘‘ping’’ sound, and 17 healthy, randomly selected Holstein Friesian cattle were used in group 2. Blood analyses (BHBA and Ca) were performed in group 1 and 2 after parturition.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125942386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.30704/HTTP-WWW-JIVS-NET.569912
S. Lotfollahzadeh, M. Heidari, Mohammad reza Mokber dezfouli, G. N. Boroujeni
The present study aim was to assess changes in clinical signs and plasma cytokines in calves experimentally infected with Escherchia coli and use them as a tool to diagnose colisepticemia. Ten healthy Holstein-Frisian bull calves were used for this experimental study. Experimental colisepticemia was induced in calves with intravenous injection of 1.5 × 109 CFU of O111:H8 strain of E.coli. Clinical scores were recorded before induction of septicemia, every 30 minutes for 8 hours, every hour from 8 till 12 h, every 3 hours from 12 till 24 hours after bacterial challenge. Blood samples were collected to determine plasma concentration of tumor necrosis factor-α (TNF-α) and gamma interferon gamma (IFN-γ) before and after E.coli IV injection. Blood culture was performed before and after bacterial challenge to confirm colisepticemia. The present study showed that total clinical score of the calves increased with a simultaneous significant rise in plasma concentration of TNF-α and INF-γ during septicemia period (P< 0.05). Changes in the heart and respiratory rate during septicemia and using clinical scoring are not enough to assess the magnitude of infection and disease progress; therefore, it is recommended some laboratory tests be used for better evaluation of clinical status of the septic calves
{"title":"Clinical and paraclinical changes in experimental colisepticemia in neonatal calves","authors":"S. Lotfollahzadeh, M. Heidari, Mohammad reza Mokber dezfouli, G. N. Boroujeni","doi":"10.30704/HTTP-WWW-JIVS-NET.569912","DOIUrl":"https://doi.org/10.30704/HTTP-WWW-JIVS-NET.569912","url":null,"abstract":"The present study aim was to assess changes in clinical signs and plasma cytokines in calves experimentally infected with Escherchia coli and use them as a tool to diagnose colisepticemia. Ten healthy Holstein-Frisian bull calves were used for this experimental study. Experimental colisepticemia was induced in calves with intravenous injection of 1.5 × 109 CFU of O111:H8 strain of E.coli. Clinical scores were recorded before induction of septicemia, every 30 minutes for 8 hours, every hour from 8 till 12 h, every 3 hours from 12 till 24 hours after bacterial challenge. Blood samples were collected to determine plasma concentration of tumor necrosis factor-α (TNF-α) and gamma interferon gamma (IFN-γ) before and after E.coli IV injection. Blood culture was performed before and after bacterial challenge to confirm colisepticemia. The present study showed that total clinical score of the calves increased with a simultaneous significant rise in plasma concentration of TNF-α and INF-γ during septicemia period (P< 0.05). Changes in the heart and respiratory rate during septicemia and using clinical scoring are not enough to assess the magnitude of infection and disease progress; therefore, it is recommended some laboratory tests be used for better evaluation of clinical status of the septic calves","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126888736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.30704/HTTP-WWW-JIVS-NET.568061
Irmak Köroğlu
This experiment was conducted on 40 newborn Holstein female calves comprising two experimental groups, with 20 calves in each group to investigate the effects of Macleaya cordata extract (MCE), on calf body weight and health which added to milk replacer. In both group calves fed on the same terms. Differently, 10 g /head/ day dosages MCE added to the milk replacer of the trial group from day 3 to day 25. Afterward, calves continued to feed until 3 months old and weighed on the birth, 15th, 40th, 60th and 90th days. At the end of the experiment, there was no significant difference at the weights on the birth and 15th days. However, on the 40th, 60th and 90th days weighing in favor of the control group, a significant difference was found (P<0.05). At the end of the experiment in favor of the trial group, there was a significant difference with clinical pneumonia and aspiration pneumonia (P<0.05). The results obtained in the research; MCE which added the milk replacer as a feed additive indicated no positive effect on calf body weight. From a health point of view, MCE has reduced the incidence of respiratory diseases in particular.
本试验以40头新生荷斯坦母犊牛为试验对象，分为2个试验组，每组20头，研究在代乳剂中添加麦克利亚籽提取物(MCE)对犊牛体重和健康状况的影响。两组小牛的喂养条件相同。试验组在第3天至第25天在代乳粉中添加10 g /头/天的MCE。之后，小牛继续进食直到3个月大，并在出生、第15、40、60和90天称重。试验结束时，出生和第15天的体重差异不显著。但在第40、60、90天，对照组体重偏重，差异有统计学意义(P<0.05)。实验结束时，试验组与临床肺炎、吸入性肺炎有显著性差异(P<0.05)。研究结果;添加代乳剂作为饲料添加剂的MCE对犊牛体重无显著影响。从健康的角度来看，MCE特别减少了呼吸系统疾病的发病率。
{"title":"Effect of milk replacer added Macleaya cordota extract calf body weight and health","authors":"Irmak Köroğlu","doi":"10.30704/HTTP-WWW-JIVS-NET.568061","DOIUrl":"https://doi.org/10.30704/HTTP-WWW-JIVS-NET.568061","url":null,"abstract":"This experiment was conducted on 40 newborn Holstein female calves comprising two experimental groups, with 20 calves in each group to investigate the effects of Macleaya cordata extract (MCE), on calf body weight and health which added to milk replacer. In both group calves fed on the same terms. Differently, 10 g /head/ day dosages MCE added to the milk replacer of the trial group from day 3 to day 25. Afterward, calves continued to feed until 3 months old and weighed on the birth, 15th, 40th, 60th and 90th days. At the end of the experiment, there was no significant difference at the weights on the birth and 15th days. However, on the 40th, 60th and 90th days weighing in favor of the control group, a significant difference was found (P<0.05). At the end of the experiment in favor of the trial group, there was a significant difference with clinical pneumonia and aspiration pneumonia (P<0.05). The results obtained in the research; MCE which added the milk replacer as a feed additive indicated no positive effect on calf body weight. From a health point of view, MCE has reduced the incidence of respiratory diseases in particular.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131220433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.30704/http-www-jivs-net.575285
V. Nasiri, F. Jameie
Here we studied the intestinal parasites in animals at a zoological garden in Alborz, Iran. A total of 83 fecal samples from various captive wild animals, consisting of 21 different species were collected randomly and analyzed for the presence of the different stages of parasites by direct smear preparation and zinc sulfate flotation followed by Ziel-Neelsen staining method. The examined animals in this study consist of 7 species of carnivores (26 samples), 10 species of herbivores (46 samples), and 4 species of different groups of birds (11 samples). Examination of fecal samples revealed that 22 (26.50%) of animals, that belonging to 7 animal species, were infected with different intestinal parasites. Among gastrointestinal parasites positive captive wild animals 18 samples (21.68 %) belong to herbivores and 4 samples (4.81 %) to Aves. Among captive wild animals the prevalence of parasites was higher in herbivores (21.68%) followed by Aves (4.81). Results indicated that out of 22 animal samples that parasites were encountered, 14 (16.86%) were infected with helminths (Trichuris spp., Nematodirus spp., Ascaridia galli and some unknown Nematodes eggs) and 8 (9.63%) were infected with protozoa (Oocysts of Eimeria sp.). In the conclusion, it could be resulted that there is a need of control measures against the spread of infectious parasitic diseases among animals within the zoo .
{"title":"Intestinal parasitic infection in wild animals of a zoological garden in Alborz, Iran","authors":"V. Nasiri, F. Jameie","doi":"10.30704/http-www-jivs-net.575285","DOIUrl":"https://doi.org/10.30704/http-www-jivs-net.575285","url":null,"abstract":"Here we studied the intestinal parasites in animals at a zoological garden in Alborz, Iran. A total of 83 fecal samples from various captive wild animals, consisting of 21 different species were collected randomly and analyzed for the presence of the different stages of parasites by direct smear preparation and zinc sulfate flotation followed by Ziel-Neelsen staining method. The examined animals in this study consist of 7 species of carnivores (26 samples), 10 species of herbivores (46 samples), and 4 species of different groups of birds (11 samples). Examination of fecal samples revealed that 22 (26.50%) of animals, that belonging to 7 animal species, were infected with different intestinal parasites. Among gastrointestinal parasites positive captive wild animals 18 samples (21.68 %) belong to herbivores and 4 samples (4.81 %) to Aves. Among captive wild animals the prevalence of parasites was higher in herbivores (21.68%) followed by Aves (4.81). Results indicated that out of 22 animal samples that parasites were encountered, 14 (16.86%) were infected with helminths (Trichuris spp., Nematodirus spp., Ascaridia galli and some unknown Nematodes eggs) and 8 (9.63%) were infected with protozoa (Oocysts of Eimeria sp.). In the conclusion, it could be resulted that there is a need of control measures against the spread of infectious parasitic diseases among animals within the zoo .","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115994544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-30DOI: 10.30704/HTTP-WWW-JIVS-NET.518064
N. Dankevych
The objective of this study was to examine the quality and sanitary characteristics after post slaughter period of the carcasses of chickens which fed with marine hydrobiont. A total of 240, 12 day-old broiler chickens from Ross 308 breed were used in the study. The chickens were divided into three groups as two experimental groups and a control group. The chickens in the control group were fed with basal diet (BD) during the experiment. In the first experimental group, 7% of the basal diet was replaced with mineral additive (MA) while in the 2nd group 7% percent protein mineral additive was added to the feed of chickens. The chicks were fed with these feeds from 21 to 42 days of age. During the study the broilers were constantly observed considering the disposal of feed consumption and their general conditions. The chickens were slaughtered at the end of the study and following slaughtering process morphologic and sanitary assessment of carcasses was carried out. In addition, external appearance, visceral organs and carcass samples of slaughtered chickens were checked during the veterinary inspection. The microbiological investigation were performed by using Colpoda steinii infusorium. There was no abnormal changes in organs or appearance of tissues among control and experimental groups according to the post slaughter veterinary inspection. In addition, visually examination revealed that carcasses were identical for the control group and the experimental groups. There was no difference between the control and experimental group according to microbial contamination results. As a conclusion, it is evaluated that the meat of broiler chicken in the experimental groups are safe for consuming. Therefore, it was concluded that the prepared hydrobionts could be used safely in the poultry feeding.
{"title":"Examination of the carcass in terms of quality and sanitation in broiler chickens fed with marine hydrobionts","authors":"N. Dankevych","doi":"10.30704/HTTP-WWW-JIVS-NET.518064","DOIUrl":"https://doi.org/10.30704/HTTP-WWW-JIVS-NET.518064","url":null,"abstract":"The objective of this study was to examine the quality and sanitary characteristics after post slaughter period of the carcasses of chickens which fed with marine hydrobiont. A total of 240, 12 day-old broiler chickens from Ross 308 breed were used in the study. The chickens were divided into three groups as two experimental groups and a control group. The chickens in the control group were fed with basal diet (BD) during the experiment. In the first experimental group, 7% of the basal diet was replaced with mineral additive (MA) while in the 2nd group 7% percent protein mineral additive was added to the feed of chickens. The chicks were fed with these feeds from 21 to 42 days of age. During the study the broilers were constantly observed considering the disposal of feed consumption and their general conditions. The chickens were slaughtered at the end of the study and following slaughtering process morphologic and sanitary assessment of carcasses was carried out. In addition, external appearance, visceral organs and carcass samples of slaughtered chickens were checked during the veterinary inspection. The microbiological investigation were performed by using Colpoda steinii infusorium. There was no abnormal changes in organs or appearance of tissues among control and experimental groups according to the post slaughter veterinary inspection. In addition, visually examination revealed that carcasses were identical for the control group and the experimental groups. There was no difference between the control and experimental group according to microbial contamination results. As a conclusion, it is evaluated that the meat of broiler chicken in the experimental groups are safe for consuming. Therefore, it was concluded that the prepared hydrobionts could be used safely in the poultry feeding.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114043593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-30DOI: 10.30704/HTTP-WWW-JIVS-NET.489172
G. Demirel
The effects of diet and levels of dietary vitamin E on lipid oxidation were assessed in lambs in this study. Groups of Suffolk x Lleyn and Scottish Blackface male lambs were fed dietary lipid supplements containing either Megalac (C16:0), or one of two sources of n-3 PUFA: linseed which has a high content of C18:3 n-3, which had been treated with formaldehyde to aid rumen bypass and a mixture of formaldehyde treated linseed plus fish oil to provide EPA and DHA. The diets were based on dried grass had similar levels of fat (60g/kg DM). Vitamin E was included as α- tocopherol acetate at 100 and 500 mg/kg, for the low and high vitamin E diets, respectively. The six dietary treatments were: Megalac with low vitamin E, (ML); Megalac with high vitamin E, (MH); Protected linseed with low vitamin E, (LL); Protected linseed with high vitamin E, (LH); Protected linseed plus fish oil (linfish) with low vitamin E, (LFL); Protected linseed plus fish oil mixture (linfish) with high vitamin E, (LFH). At approximately half of the mature live weight for each breed, animals were slaughtered. This was on average 46 kg for the Suffolk and 36 kg for the Scottish Blackface. The meat from supplemented animals increased susceptibility to lipid oxidation in high PUFA in meat resulted from poor deposition of dietary vitamin E supplements.
{"title":"Effects of dietary fat source, breed and vitamin E level on lipid oxidation of muscles from sheep","authors":"G. Demirel","doi":"10.30704/HTTP-WWW-JIVS-NET.489172","DOIUrl":"https://doi.org/10.30704/HTTP-WWW-JIVS-NET.489172","url":null,"abstract":"The effects of diet and levels of dietary vitamin E on lipid oxidation were assessed in lambs in this study. Groups of Suffolk x Lleyn and Scottish Blackface male lambs were fed dietary lipid supplements containing either Megalac (C16:0), or one of two sources of n-3 PUFA: linseed which has a high content of C18:3 n-3, which had been treated with formaldehyde to aid rumen bypass and a mixture of formaldehyde treated linseed plus fish oil to provide EPA and DHA. The diets were based on dried grass had similar levels of fat (60g/kg DM). Vitamin E was included as α- tocopherol acetate at 100 and 500 mg/kg, for the low and high vitamin E diets, respectively. The six dietary treatments were: Megalac with low vitamin E, (ML); Megalac with high vitamin E, (MH); Protected linseed with low vitamin E, (LL); Protected linseed with high vitamin E, (LH); Protected linseed plus fish oil (linfish) with low vitamin E, (LFL); Protected linseed plus fish oil mixture (linfish) with high vitamin E, (LFH). At approximately half of the mature live weight for each breed, animals were slaughtered. This was on average 46 kg for the Suffolk and 36 kg for the Scottish Blackface. The meat from supplemented animals increased susceptibility to lipid oxidation in high PUFA in meat resulted from poor deposition of dietary vitamin E supplements.","PeriodicalId":142259,"journal":{"name":"Journal of Istanbul Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134229146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-30DOI: 10.30704/HTTP-WWW-JIVS-NET.529095
L. Koenhemsi, R. Gönül
Chronic renal failure (CRF) is an important cause of morbidity and mortality in dogs. The aim of this study is to investigate the renal Doppler measurements, blood hypertension and acid-base levels in dogs with chronic renal failure. Twenty-six dogs previously diagnosed with chronic renal failure were compared with twenty healthy dogs. A complete blood cell, biochemical profile, urinalysis, blood gasses, blood pressure were analyzed and changes in renal blood flow were measured by renal Doppler ultrasonography. The dogs with CRF had significantly higher serum blood urea nitrogen, creatinine and phosphorus concentration, significantly lower packed cell volume, and urine specific gravity than control dogs. Positive correlation was determined between serum creatinine levels and renal Doppler indices. There were significant decreases in blood pH and bicarbonate. Indirect blood pressure measurements were slightly increased in CRF dogs. Renal Doppler measurement was observed as a helpful tool in diagnosing the CRF in dogs. Identification of acidosis and hypertension may help in developing treatments that slow the rate of progression of chronic renal failure.
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Pub Date : 2019-04-30DOI: 10.30704/HTTP-WWW-JIVS-NET.551979
M. Kati̇ca, Alisa Smajović, Nasreldin Hassan Ahmed, B. Dukić, R. Baljic
Bites of laboratory animals are treated as the bites of any other animals since the possibility of existence of pathogenic microorganisms, unfavorable for human health, in the rooms where these animals reside, is not excluded. A rare case of a laboratory rat bite, used for scientific research and previously infected with Pseudomonas aeruginosa , is presented here. The patient's wound was located on the forefinger of his hand and was 1 cm long and up to 0.2 cm deep. The antitetanus prophylaxis was administered in an ambulance, including antibiotic therapy with amoxicillin-clavulanate per os. There exists a need for checks and special guidelines for the handling and retention of laboratory animals. The patient has successfully remedied the wound, thanks to consistent antibiotic therapy and antitetanus prophylaxis, and possible inflammatory complications were prevented .
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