Pub Date : 2021-06-01DOI: 10.26577/eje.2021.v67.i2.01
Y. A. Kumar, K. Sheryazdanova
{"title":"Ecotourism study in Kazakhstan: the past, present and the future","authors":"Y. A. Kumar, K. Sheryazdanova","doi":"10.26577/eje.2021.v67.i2.01","DOIUrl":"https://doi.org/10.26577/eje.2021.v67.i2.01","url":null,"abstract":"","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127184580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.26577/eje.2021.v67.i2.02
Y. Ussipbekova, А. B. Rakhym, G. Seilkhanova, А. D. Zhagiparova, А. К. Kenessova, G. О. Osmanzhan
{"title":"Investigation of the adsorption of copper and zinc ions by sorbents based on plant waste – cake and husk of sunflower seeds","authors":"Y. Ussipbekova, А. B. Rakhym, G. Seilkhanova, А. D. Zhagiparova, А. К. Kenessova, G. О. Osmanzhan","doi":"10.26577/eje.2021.v67.i2.02","DOIUrl":"https://doi.org/10.26577/eje.2021.v67.i2.02","url":null,"abstract":"","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114185532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.26577/eje.2021.v67.i2.08
U. Erezhetova, G. M. Sultangalyeva, N. Terletskaya, M. Kurmanbayeva, M. Hoffmann
{"title":"Anatomical parameters of the flag leaf of alloplasmic lines and their parental forms in drought conditions","authors":"U. Erezhetova, G. M. Sultangalyeva, N. Terletskaya, M. Kurmanbayeva, M. Hoffmann","doi":"10.26577/eje.2021.v67.i2.08","DOIUrl":"https://doi.org/10.26577/eje.2021.v67.i2.08","url":null,"abstract":"","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121683781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.26577/EJE.2021.V66.I1.02
A. B. Karazhanova, N. M. Kerimbek, A. Lovinskaya, S. Kolumbayeva, S. Abilev
Ecological monitoring, including water resources, is included in a set of measures for the rational use and protection of the environment. Currently, the assessment of water resources’ genotoxicity and mutagenicity is an essential component of ecological monitoring. This research aimed to investigate water’s genotoxicity from the Esentai and Ulken Almaty rivers flowing in Almaty. It was established that the content of Fe, Cu, Co, Ni, Pb, and Cd in the Almaty rivers water does not exceed the maximum permissible concentration (MPC). In contrast, zinc content in the Esentai and Ulken Almaty rivers’ water exceeded the MPC, respectively, by 1.1 and 7.7-folds, manganese – 2.1, and 1.9-folds. It was found that water samples from the Esentai and Ulken Almaty rivers showed a DNA-damaging effect on the studied cells (bone marrow, liver, kidneys, and spleen) of laboratory mice. DNA damage was determined using the Comet assay. In the cells of the studied organs in the experimental groups, the frequency of single-strand DNA breaks a statistically significant increase compared to the control group’s animal. The genotoxic action’s organospecificity of the studied river waters on laboratory mice was established. According to their sensitivity to water’s genotoxic effect, experimental mice’s organs can arrange in the following order: spleen and bone marrow> liver> kidneys. An increase in lipid peroxidation products was found in the laboratory mice’ liver that drank water from the Esentai and Ulken Almaty rivers. Thus, the results of the physicochemical, molecular-genetic, and biochemical analysis of water on animal test objects indicate the presence in the investigated natural surface waters of chemicals with genotoxic and toxic activity. окисления липидов в печени лабораторных мышей, принимавших воду из рек Есентай и Улкен Алматы. Таким образом, результаты физико-химического, молекулярно-генетического и биохимического анализа воды на животных тест-объектах обладающих Ключевые слова: поверхностные тяжелые металлы, метод ДНК-комет, органоспе ци-фичность, перекисное окисление липидов.
{"title":"Genotoxicity of esentai and ulken almaty rivers water on animal test-systems","authors":"A. B. Karazhanova, N. M. Kerimbek, A. Lovinskaya, S. Kolumbayeva, S. Abilev","doi":"10.26577/EJE.2021.V66.I1.02","DOIUrl":"https://doi.org/10.26577/EJE.2021.V66.I1.02","url":null,"abstract":"Ecological monitoring, including water resources, is included in a set of measures for the rational use and protection of the environment. Currently, the assessment of water resources’ genotoxicity and mutagenicity is an essential component of ecological monitoring. This research aimed to investigate water’s genotoxicity from the Esentai and Ulken Almaty rivers flowing in Almaty. It was established that the content of Fe, Cu, Co, Ni, Pb, and Cd in the Almaty rivers water does not exceed the maximum permissible concentration (MPC). In contrast, zinc content in the Esentai and Ulken Almaty rivers’ water exceeded the MPC, respectively, by 1.1 and 7.7-folds, manganese – 2.1, and 1.9-folds. It was found that water samples from the Esentai and Ulken Almaty rivers showed a DNA-damaging effect on the studied cells (bone marrow, liver, kidneys, and spleen) of laboratory mice. DNA damage was determined using the Comet assay. In the cells of the studied organs in the experimental groups, the frequency of single-strand DNA breaks a statistically significant increase compared to the control group’s animal. The genotoxic action’s organospecificity of the studied river waters on laboratory mice was established. According to their sensitivity to water’s genotoxic effect, experimental mice’s organs can arrange in the following order: spleen and bone marrow> liver> kidneys. An increase in lipid peroxidation products was found in the laboratory mice’ liver that drank water from the Esentai and Ulken Almaty rivers. Thus, the results of the physicochemical, molecular-genetic, and biochemical analysis of water on animal test objects indicate the presence in the investigated natural surface waters of chemicals with genotoxic and toxic activity. окисления липидов в печени лабораторных мышей, принимавших воду из рек Есентай и Улкен Алматы. Таким образом, результаты физико-химического, молекулярно-генетического и биохимического анализа воды на животных тест-объектах обладающих Ключевые слова: поверхностные тяжелые металлы, метод ДНК-комет, органоспе ци-фичность, перекисное окисление липидов.","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121430844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-22DOI: 10.26577/eje.2019.v61.i4.08
А.А. Усербаева, Бейсембек А.Е., Косалбаев Б.Д., К. Рысбекулы, Б.К. Заядан, Какимова А.Б., К. Болатхан
. The accumulation of carbon dioxide (CO 2 ) in the atmosphere, as well as pollution of water objects with heavy metals, are environmental problems associated with numerous impacts on ecosystems. The cultivation of photosynthetic microorganisms that efficiently absorb CO 2 is a promising solution, since the biomass of cyanobacteria can be used to produce valuable bioproducts. Interest in the effect of high concentrations of CO 2 on the activity of cyanobacteria is growing in connection with studies of cleaning the atmosphere from industrial pollution using cyanobacteria as biofilters, due to their ability to grow rapidly in conditions of high CO 2 content in the atmosphere. Cyanobacteria are a convenient object for research due to the high growth rate and controllability of their biosynthesis. This article presents the results of the influence of different concentrations of carbon dioxide on the productivity of strains of cyanobacteria. Resistance to inhibitory concentrations of CO 2 is species-specific and varies over a wide range of values. The effect of carbon dioxide at a concentration of 2%, 4%, and 8% on the growth rate of cyanobacteria was studied, and a comparative assessment of the effect of increased carbon dioxide concentrations on biomass growth from the readings of optical density and fluorescence activity was carried out. It was determined that carbon dioxide concentrations of 2-4% have a positive effect on the productivity of the studied cultures of cyanobacteria. It was found that increasing the concentration of CO 2 to 8% leads to a decrease in growth rates of cyanobacteria. It was determined that the strain Cyanobacterium sp. IPPAS B-1200 was selected as the most productive strain from the studied cyanobacteria according to the yield of dry biomass, fluoresce activity and characterized by active growth at a concentration of CO 2 in air – 4%.
{"title":"Effects of various CO 2 concentrations on the productivity of cyanobacterial strains","authors":"А.А. Усербаева, Бейсембек А.Е., Косалбаев Б.Д., К. Рысбекулы, Б.К. Заядан, Какимова А.Б., К. Болатхан","doi":"10.26577/eje.2019.v61.i4.08","DOIUrl":"https://doi.org/10.26577/eje.2019.v61.i4.08","url":null,"abstract":". The accumulation of carbon dioxide (CO 2 ) in the atmosphere, as well as pollution of water objects with heavy metals, are environmental problems associated with numerous impacts on ecosystems. The cultivation of photosynthetic microorganisms that efficiently absorb CO 2 is a promising solution, since the biomass of cyanobacteria can be used to produce valuable bioproducts. Interest in the effect of high concentrations of CO 2 on the activity of cyanobacteria is growing in connection with studies of cleaning the atmosphere from industrial pollution using cyanobacteria as biofilters, due to their ability to grow rapidly in conditions of high CO 2 content in the atmosphere. Cyanobacteria are a convenient object for research due to the high growth rate and controllability of their biosynthesis. This article presents the results of the influence of different concentrations of carbon dioxide on the productivity of strains of cyanobacteria. Resistance to inhibitory concentrations of CO 2 is species-specific and varies over a wide range of values. The effect of carbon dioxide at a concentration of 2%, 4%, and 8% on the growth rate of cyanobacteria was studied, and a comparative assessment of the effect of increased carbon dioxide concentrations on biomass growth from the readings of optical density and fluorescence activity was carried out. It was determined that carbon dioxide concentrations of 2-4% have a positive effect on the productivity of the studied cultures of cyanobacteria. It was found that increasing the concentration of CO 2 to 8% leads to a decrease in growth rates of cyanobacteria. It was determined that the strain Cyanobacterium sp. IPPAS B-1200 was selected as the most productive strain from the studied cyanobacteria according to the yield of dry biomass, fluoresce activity and characterized by active growth at a concentration of CO 2 in air – 4%.","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127373154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-22DOI: 10.26577/EJE.2019.V61.I4.01
U. Aliyaskarova, M. Saparbaev, A. Bissenbaev
Abstract. Interstrand crosslinks (ICLs) occur when two complimentary strands of DNA are covalentlylinked together after exposure to crosslinking agents, therefore blocking the processes essential for cellsurvival such as DNA transcription, replication and recombination by preventing the strand separationand switching cell fate to apoptosis. Taking advantage of it, chemical agents such as cisplatin, mitomycinC and nitrogen mustards are widely used in chemotherapy against cancer and several hyperplasic diseases. However, cellular responses induced by ICLs and repair mechanisms counteracting their cytotoxiceffect can lead to the appearance of acquired resistance in cancer cells thus limiting the efficiency of thetreatment. In this review, we will discuss the main properties of several classes of ICL-forming agents andrecent advances in our understanding of the mechanisms of ICL repair. Due to the recent developmentson the repair mechanisms of various ICLs, our insight has broadened regarding the drug-specific formation and cellular processing of ICLs. Even though the main features of ICL repair remained the same, newplayers of repair machinery acting upon specific ICLs are being discovered. These new findings mayfurnish a basis to improve and adapt anticancer therapies by targeting DNA repair pathways in order tocounteract the development of resistance to anti-cancer treatments.Key words: DNA repair, oxidative stress, DNA crosslinks
{"title":"Repair of interstrand DNA crosslinks induced by oxidative stress and anti-cancer agents","authors":"U. Aliyaskarova, M. Saparbaev, A. Bissenbaev","doi":"10.26577/EJE.2019.V61.I4.01","DOIUrl":"https://doi.org/10.26577/EJE.2019.V61.I4.01","url":null,"abstract":"Abstract. Interstrand crosslinks (ICLs) occur when two complimentary strands of DNA are covalentlylinked together after exposure to crosslinking agents, therefore blocking the processes essential for cellsurvival such as DNA transcription, replication and recombination by preventing the strand separationand switching cell fate to apoptosis. Taking advantage of it, chemical agents such as cisplatin, mitomycinC and nitrogen mustards are widely used in chemotherapy against cancer and several hyperplasic diseases. However, cellular responses induced by ICLs and repair mechanisms counteracting their cytotoxiceffect can lead to the appearance of acquired resistance in cancer cells thus limiting the efficiency of thetreatment. In this review, we will discuss the main properties of several classes of ICL-forming agents andrecent advances in our understanding of the mechanisms of ICL repair. Due to the recent developmentson the repair mechanisms of various ICLs, our insight has broadened regarding the drug-specific formation and cellular processing of ICLs. Even though the main features of ICL repair remained the same, newplayers of repair machinery acting upon specific ICLs are being discovered. These new findings mayfurnish a basis to improve and adapt anticancer therapies by targeting DNA repair pathways in order tocounteract the development of resistance to anti-cancer treatments.Key words: DNA repair, oxidative stress, DNA crosslinks","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125664362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-22DOI: 10.26577/eje.2019.v61.i4.05
A. M. Kalimagambetov, A. Dyusembaeva, I. A. Khalelova, Z. Aitasheva, B. Kudryavtsev
. The article presents the data of cytogenetic study on the fetal karyotype of 310 pregnant women from the city Aktau in 2013-2015. Chorionic biopsy and placental biopsy were carried out for 248 pregnant women with amniocentesis in 18 cases and cordocentesis in 44 cases. Clinical indicators imposing invasive procedures to pregnant women at risk were maternal age factor, fetal ultrasound markers, serum blood markers determined in pregnant women, the presence of children with multiple congenital malformations, chromosomal pathologies, etc. Distribution frequencies of fetal chromosomal abnormalities have been determined based on clinical and laboratory studies. The highest frequency of chromosomal abnormalities of the fetus was indicated in pregnant women with three salient indicators: the factor of the age of the pregnant woman, parameters of biochemical screening, and ultrasound markers. Chromosomal abnormalities of the fetus were detected in 94 (30.3%) pregnant women including 92 cases (97.9%) represented by numerical chromosome disorders and 2 cases (2.1%) of structural disorders. Disorders of the autosome system were observed 9.4 times more often comparing to abnormalities in the sex chromosome system. Of the numerical chromosome abnormalities, a high specific weight is occupied by trisomy of the 21-st chromosome which has reached 65.1%. A comparative analysis of the frequencies of chromosomal abnormalities of the fetus of two port cities has demonstrated a 1.7-fold increase in the city of Aktau (Kazakhstan) compared with the city of Murmansk (Russia), and the average frequencies of fetal karyotype anomalies have made up 19.6% and 11.6%, respectively. Higher level of frequencies for fetal karyotype anomalies in Aktau is possibly associated with unfavorable environmental conditions in this city caused by the allocation of the oil and gas industry, the repository for tailing toxic and radioactive wastes, and abandoned uranium mines.
{"title":"Chromosomal disorders in the fetus of pregnant woman from Aktau","authors":"A. M. Kalimagambetov, A. Dyusembaeva, I. A. Khalelova, Z. Aitasheva, B. Kudryavtsev","doi":"10.26577/eje.2019.v61.i4.05","DOIUrl":"https://doi.org/10.26577/eje.2019.v61.i4.05","url":null,"abstract":". The article presents the data of cytogenetic study on the fetal karyotype of 310 pregnant women from the city Aktau in 2013-2015. Chorionic biopsy and placental biopsy were carried out for 248 pregnant women with amniocentesis in 18 cases and cordocentesis in 44 cases. Clinical indicators imposing invasive procedures to pregnant women at risk were maternal age factor, fetal ultrasound markers, serum blood markers determined in pregnant women, the presence of children with multiple congenital malformations, chromosomal pathologies, etc. Distribution frequencies of fetal chromosomal abnormalities have been determined based on clinical and laboratory studies. The highest frequency of chromosomal abnormalities of the fetus was indicated in pregnant women with three salient indicators: the factor of the age of the pregnant woman, parameters of biochemical screening, and ultrasound markers. Chromosomal abnormalities of the fetus were detected in 94 (30.3%) pregnant women including 92 cases (97.9%) represented by numerical chromosome disorders and 2 cases (2.1%) of structural disorders. Disorders of the autosome system were observed 9.4 times more often comparing to abnormalities in the sex chromosome system. Of the numerical chromosome abnormalities, a high specific weight is occupied by trisomy of the 21-st chromosome which has reached 65.1%. A comparative analysis of the frequencies of chromosomal abnormalities of the fetus of two port cities has demonstrated a 1.7-fold increase in the city of Aktau (Kazakhstan) compared with the city of Murmansk (Russia), and the average frequencies of fetal karyotype anomalies have made up 19.6% and 11.6%, respectively. Higher level of frequencies for fetal karyotype anomalies in Aktau is possibly associated with unfavorable environmental conditions in this city caused by the allocation of the oil and gas industry, the repository for tailing toxic and radioactive wastes, and abandoned uranium mines.","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132947799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-27DOI: 10.26577/eje.2019.v60.i3.05
Л.Ж. Гумарова, Э.Р. Бикинеева, Н. Т. Аблайханова, Л.К. Бактыбаева, А. Ыдырыс
{"title":"Influence of the natural solar radiation in the mountains and artificial light on the circadian rhythms of motor activity","authors":"Л.Ж. Гумарова, Э.Р. Бикинеева, Н. Т. Аблайханова, Л.К. Бактыбаева, А. Ыдырыс","doi":"10.26577/eje.2019.v60.i3.05","DOIUrl":"https://doi.org/10.26577/eje.2019.v60.i3.05","url":null,"abstract":"","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130229107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Spring waters, as natural discharges of groundwater to the surface, are an essential component of the natural environment. Spring water is considered by nature to be exceptionally pure, transparent, tasty, and balanced in its chemical composition, which makes it attractive to humans. However, the quality of spring water in urban areas has deteriorated significantly due to the high risk of chemical and bacterial contaminants entering groundwater. Therefore, studies were carried out of spring waters, actively consumed by the population of Almaty, for toxic and mutagenic activity. Spring 1 is located near the river Prohodnaya, bus stop number 28; spring 2 – on the road to the Big Almaty Lake, in front of the bridge over the river Ulken Almaty; spring 3 – near the river Batareika on the road to Kok-Zhailau; spring 4 – at the Prosveshchenets stop on the road to Medeu. The excess of MPC for lead in the water of springs 1, 3, and 4 has been established. Following the established MPC for heavy metals, water from spring 2 belongs to quality class 1 and is suitable for all types of water use; water from springs 1, 3 and 4 belongs to quality class 2. The waters of this water use’ class are suitable for all categories of water use, except for domestic. A bioluminescent test revealed high toxicity of spring waters 1-3, which significantly reduced the bioluminescent response of RecA and ColD biosensors (p <0.05). In the Allium test, the water of all the springs have phytotoxic activity, water from springs 1 and 3 also have a genotoxic effect. In the chromosomal aberration assay in H. vulgare, it was found that the water of spring-3 had a mutagenic activity. There was a statistically significant increase in the frequency of chromosomal aberrations in barley seeds (p <0.01). The remaining studied waters also increased the frequency of aberrant cells compared with the control; however, the difference was not statistically significant. Besides, polyploid cells (more than 1.5%) were identified with high frequency in the root meristem of barley seeds germinated in water from springs 1 and 3. The research indicates the presence of mutagenic factors in the waters of these springs. A comparative analysis of the level of contamination of the studied springs with heavy metals, the level of toxic and mutagenic activity of water allows ranking the investigated water objects in the following order: spring 3> spring 1> spring 2> spring 4.
{"title":"Toxic and mutagenic activity of the spring waters of Almaty","authors":"Lovinskaya A.V, Kolumbayeva S.Zh., Suvorova M.A, Iliyasova A.I, Abilev S.K","doi":"10.26577/eje.2019.v60.i3.04","DOIUrl":"https://doi.org/10.26577/eje.2019.v60.i3.04","url":null,"abstract":"Spring waters, as natural discharges of groundwater to the surface, are an essential component of the natural environment. Spring water is considered by nature to be exceptionally pure, transparent, tasty, and balanced in its chemical composition, which makes it attractive to humans. However, the quality of spring water in urban areas has deteriorated significantly due to the high risk of chemical and bacterial contaminants entering groundwater. Therefore, studies were carried out of spring waters, actively consumed by the population of Almaty, for toxic and mutagenic activity. Spring 1 is located near the river Prohodnaya, bus stop number 28; spring 2 – on the road to the Big Almaty Lake, in front of the bridge over the river Ulken Almaty; spring 3 – near the river Batareika on the road to Kok-Zhailau; spring 4 – at the Prosveshchenets stop on the road to Medeu. The excess of MPC for lead in the water of springs 1, 3, and 4 has been established. Following the established MPC for heavy metals, water from spring 2 belongs to quality class 1 and is suitable for all types of water use; water from springs 1, 3 and 4 belongs to quality class 2. The waters of this water use’ class are suitable for all categories of water use, except for domestic. A bioluminescent test revealed high toxicity of spring waters 1-3, which significantly reduced the bioluminescent response of RecA and ColD biosensors (p <0.05). In the Allium test, the water of all the springs have phytotoxic activity, water from springs 1 and 3 also have a genotoxic effect. In the chromosomal aberration assay in H. vulgare, it was found that the water of spring-3 had a mutagenic activity. There was a statistically significant increase in the frequency of chromosomal aberrations in barley seeds (p <0.01). The remaining studied waters also increased the frequency of aberrant cells compared with the control; however, the difference was not statistically significant. Besides, polyploid cells (more than 1.5%) were identified with high frequency in the root meristem of barley seeds germinated in water from springs 1 and 3. The research indicates the presence of mutagenic factors in the waters of these springs. A comparative analysis of the level of contamination of the studied springs with heavy metals, the level of toxic and mutagenic activity of water allows ranking the investigated water objects in the following order: spring 3> spring 1> spring 2> spring 4.","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126105657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-27DOI: 10.26577/eje.2019.v60.i3.08
{"title":"Selection of primers for the assessment of nodular dermatitis in cattle using the method of polymerase chain reaction","authors":"","doi":"10.26577/eje.2019.v60.i3.08","DOIUrl":"https://doi.org/10.26577/eje.2019.v60.i3.08","url":null,"abstract":"","PeriodicalId":143778,"journal":{"name":"Eurasian Journal of Ecology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123061820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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