首页 > 最新文献

Iranian journal of allergy, asthma, and immunology最新文献

英文 中文
Immune Profiling of SARS-CoV-2; What We Know and What We Don't Know. SARS-CoV-2的免疫特征分析我们知道的和我们不知道的。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-06-16 DOI: 10.18502/ijaai.v22i3.13050
Mahshid Zohouri, Atri Ghods, Mina Roshan Zamir, Fazel Shokri, Abbas Ghaderi

Coronavirus disease 2019 (COVID-19), described as World War 3, is the current worldwide health challenge and nearly all countries have so far faced this disaster. There is still no cure because of the complicated pathogenesis, however, there are several studies on track investigating different aspects of the immune response to the virus. In this review, we will provide an overview of recent investigations that have analyzed immune cells in patients with COVID-19. We will then discuss the differences in immune profiles between healthy controls and various clinical presentations, including asymptomatic, mild, moderate, and severe cases.

被称为第三次世界大战的2019冠状病毒病(COVID-19)是当前全球卫生挑战,迄今几乎所有国家都面临着这场灾难。由于其复杂的发病机制,目前还没有治愈的方法,然而,有几项研究正在研究对病毒的免疫反应的不同方面。在这篇综述中,我们将概述最近分析COVID-19患者免疫细胞的研究。然后,我们将讨论健康对照者和各种临床表现(包括无症状、轻度、中度和重度病例)之间免疫概况的差异。
{"title":"Immune Profiling of SARS-CoV-2; What We Know and What We Don't Know.","authors":"Mahshid Zohouri,&nbsp;Atri Ghods,&nbsp;Mina Roshan Zamir,&nbsp;Fazel Shokri,&nbsp;Abbas Ghaderi","doi":"10.18502/ijaai.v22i3.13050","DOIUrl":"https://doi.org/10.18502/ijaai.v22i3.13050","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19), described as World War 3, is the current worldwide health challenge and nearly all countries have so far faced this disaster. There is still no cure because of the complicated pathogenesis, however, there are several studies on track investigating different aspects of the immune response to the virus. In this review, we will provide an overview of recent investigations that have analyzed immune cells in patients with COVID-19. We will then discuss the differences in immune profiles between healthy controls and various clinical presentations, including asymptomatic, mild, moderate, and severe cases.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 3","pages":"217-232"},"PeriodicalIF":1.5,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9908286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
IL-25 Impact on Malignant B Cells Survival and T Cells Activation in Chronic Lymphocytic Leukemia. IL-25对慢性淋巴细胞白血病恶性B细胞存活和T细胞活化的影响
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-06-16 DOI: 10.18502/ijaai.v22i3.13058
Mehrnoosh Pashei, Farahnaz Ghahremanfard, Ehsan Manouchehri Doulabi, Maral Hemmati, Fatemeh Pak, Parviz Kokhaei

T cell dysregulation and shift to T helper 2 responses, boosting tumor microenvironment support, contributes to the survival of leukemic B cells in Chronic Lymphocytic Leukemia. Interleukin (IL)-25 is involved in the initiation of T helper 2 cell responses. Signal transduction of IL-25 begins with the heterodimer receptor (IL-17RA/IL-17RB). The presence of IL-25 in the tumor microenvironment may affect the supportive effects of T cells in the surrounding tumor cell environment. The purpose of this study was to evaluate the role of IL-25 in the biology of CLL. IL-17RB expression in CD3+ and CD19+ cells was assessed in isolated peripheral blood mononuclear cells (PBMCs) of nine CLL patients and nine healthy subjects by real-time polymerase chain reaction and flow cytometry. B cells were positively enriched from PBMCs using magnetic-activated cell sorting (MACS). PBMCs and purified leukemic B cells were cultured with recombinant human IL-25 (20ng/ml) for 72 hours, then the viability and apoptosis of cultured cells were measured by MTT assay and AnnexinV/7AAD. Furthermore, the levels of CD69 expression on T lymphocytes and IL-17RB in T and B cells were determined by flow cytometry. The basal level of IL-17RB expression in CLL patients was significantly higher than that in control individuals. In addition, the percentage of IL-17RB+/CD3+, IL-17RB+/CD19+ cells and CD69+/CD3+ cells increased after 72 hours of culture with IL-25 in CLL patients compared to healthy subjects. IL-25 also reduces the apoptosis rate of tumor cells. We found that IL-25 could stimulate T cells in CLL patients and lower B cell death. This suggests that IL-25 might have a role in enhancing the survival of tumor cell by expressing receptors for inflammation, such as IL-17RB, and might be involved in the development of CLL.

在慢性淋巴细胞白血病中,T细胞失调和向辅助性T细胞2反应的转移,增强肿瘤微环境支持,有助于白血病B细胞的存活。白细胞介素(IL)-25参与T辅助2细胞反应的启动。IL-25的信号转导始于异源二聚体受体(IL-17RA/IL-17RB)。肿瘤微环境中IL-25的存在可能会影响T细胞对周围肿瘤细胞环境的支持作用。本研究的目的是评估IL-25在CLL生物学中的作用。采用实时聚合酶链反应和流式细胞术检测9例慢性淋巴细胞白血病患者和9例健康人外周血单个核细胞(PBMCs)中CD3+和CD19+细胞中IL-17RB的表达。利用磁激活细胞分选(MACS)技术从PBMCs中富集B细胞。用重组人IL-25 (20ng/ml)培养pbmc和纯化的白血病B细胞72h,采用MTT法和AnnexinV/7AAD检测培养细胞的活力和凋亡情况。流式细胞术检测T淋巴细胞CD69表达水平,T细胞和B细胞IL-17RB表达水平。CLL患者IL-17RB基础表达水平明显高于对照组。此外,与健康受试者相比,CLL患者IL-25培养72小时后,IL-17RB+/CD3+、IL-17RB+/CD19+细胞和CD69+/CD3+细胞的百分比增加。IL-25还能降低肿瘤细胞的凋亡率。我们发现IL-25可以刺激CLL患者的T细胞并降低B细胞死亡。这表明IL-25可能通过表达IL-17RB等炎症受体来提高肿瘤细胞的存活,并可能参与CLL的发展。
{"title":"IL-25 Impact on Malignant B Cells Survival and T Cells Activation in Chronic Lymphocytic Leukemia.","authors":"Mehrnoosh Pashei,&nbsp;Farahnaz Ghahremanfard,&nbsp;Ehsan Manouchehri Doulabi,&nbsp;Maral Hemmati,&nbsp;Fatemeh Pak,&nbsp;Parviz Kokhaei","doi":"10.18502/ijaai.v22i3.13058","DOIUrl":"https://doi.org/10.18502/ijaai.v22i3.13058","url":null,"abstract":"<p><p>T cell dysregulation and shift to T helper 2 responses, boosting tumor microenvironment support, contributes to the survival of leukemic B cells in Chronic Lymphocytic Leukemia. Interleukin (IL)-25 is involved in the initiation of T helper 2 cell responses. Signal transduction of IL-25 begins with the heterodimer receptor (IL-17RA/IL-17RB). The presence of IL-25 in the tumor microenvironment may affect the supportive effects of T cells in the surrounding tumor cell environment. The purpose of this study was to evaluate the role of IL-25 in the biology of CLL. IL-17RB expression in CD3+ and CD19+ cells was assessed in isolated peripheral blood mononuclear cells (PBMCs) of nine CLL patients and nine healthy subjects by real-time polymerase chain reaction and flow cytometry. B cells were positively enriched from PBMCs using magnetic-activated cell sorting (MACS). PBMCs and purified leukemic B cells were cultured with recombinant human IL-25 (20ng/ml) for 72 hours, then the viability and apoptosis of cultured cells were measured by MTT assay and AnnexinV/7AAD. Furthermore, the levels of CD69 expression on T lymphocytes and IL-17RB in T and B cells were determined by flow cytometry. The basal level of IL-17RB expression in CLL patients was significantly higher than that in control individuals. In addition, the percentage of IL-17RB+/CD3+, IL-17RB+/CD19+ cells and CD69+/CD3+ cells increased after 72 hours of culture with IL-25 in CLL patients compared to healthy subjects. IL-25 also reduces the apoptosis rate of tumor cells. We found that IL-25 could stimulate T cells in CLL patients and lower B cell death. This suggests that IL-25 might have a role in enhancing the survival of tumor cell by expressing receptors for inflammation, such as IL-17RB, and might be involved in the development of CLL.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 3","pages":"299-311"},"PeriodicalIF":1.5,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9925402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Characteristics and Economic Burden of Asthma in China: A Multicenter Retrospective Study. 中国哮喘的临床特征和经济负担:一项多中心回顾性研究。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-06-16 DOI: 10.18502/ijaai.v22i3.13057
Bin Shang, Xiangguo Li, Yu Xu, Wenxin Ren, Junren Wang, Chunyan Xing, Shujuan Jiang, Jian Sun

Asthma is a common chronic airway inflammation that produces a healthcare burden on the economy. We aim to obtain a better understanding of the clinical status and disease burden of patients with asthma in China. A retrospective study was carried out based on the computerized medical records in the Jinan Health Medical Big Data Platform between 2011 and 2019 (available data from 38 hospitals). The asthma severity of each patient was assessed retrospectively and categorized as mild, moderate, or severe according to Global Initiative for Asthma 2020 (GINA 2020). The results revealed that the majority (75.0%) of patients suffered from mild asthma. Patients treated with inhaled corticosteroids (ICS)/long-acting beta-agonists (LABA) at emergency department visits had lower frequencies of exacerbations compared with non-ICS/LABA-treated patients. The incidence rates for 1, 2, 3, and 4 exacerbation of the patients treated with ICS/LABA are lower than those treated without ICS/LABA (14.49 vs. 15.01%, 11.94% vs. 19.12%, 6.51% vs.12.92% and 4.10% vs. 9.35%). The difference got a statistical significance Chronic obstructive pulmonary disease (COPD) and gastroesophageal reflux disease (GERD), two comorbidities related to asthma, were risk factors for asthma exacerbation. Finally, patients who suffered from exacerbations produced a heavier economic burden compared to the patients who never suffered exacerbations (mean costs are ¥3,339.67 vs. ¥968.45 separately).  These results provide a reference for clinicians and patients to obtain a better treatment and therapy strategy management for people living with asthma.

哮喘是一种常见的慢性气道炎症,对经济产生医疗负担。我们的目的是更好地了解中国哮喘患者的临床状况和疾病负担。基于济南健康医疗大数据平台2011 - 2019年计算机化病历(可查数据来自38家医院)进行回顾性研究。回顾性评估每位患者的哮喘严重程度,并根据全球哮喘倡议2020 (GINA 2020)将其分为轻度、中度或重度。结果显示,大多数(75.0%)患者患有轻度哮喘。在急诊就诊时接受吸入皮质类固醇(ICS)/长效β激动剂(LABA)治疗的患者与未接受ICS/LABA治疗的患者相比,病情恶化的频率较低。ICS/LABA组患者的1、2、3、4级加重发生率低于未使用ICS/LABA组(14.49% vs. 15.01%, 11.94% vs. 19.12%, 6.51% vs.12.92%, 4.10% vs. 9.35%)。慢性阻塞性肺疾病(COPD)和胃食管反流病(GERD)两种与哮喘相关的合并症是哮喘加重的危险因素。最后,发生急性加重的患者比未发生急性加重的患者产生了更大的经济负担(平均费用分别为¥3,339.67元和¥968.45)。这些结果为临床医生和患者获得更好的哮喘治疗和治疗策略管理提供了参考。
{"title":"Clinical Characteristics and Economic Burden of Asthma in China: A Multicenter Retrospective Study.","authors":"Bin Shang,&nbsp;Xiangguo Li,&nbsp;Yu Xu,&nbsp;Wenxin Ren,&nbsp;Junren Wang,&nbsp;Chunyan Xing,&nbsp;Shujuan Jiang,&nbsp;Jian Sun","doi":"10.18502/ijaai.v22i3.13057","DOIUrl":"https://doi.org/10.18502/ijaai.v22i3.13057","url":null,"abstract":"<p><p>Asthma is a common chronic airway inflammation that produces a healthcare burden on the economy. We aim to obtain a better understanding of the clinical status and disease burden of patients with asthma in China. A retrospective study was carried out based on the computerized medical records in the Jinan Health Medical Big Data Platform between 2011 and 2019 (available data from 38 hospitals). The asthma severity of each patient was assessed retrospectively and categorized as mild, moderate, or severe according to Global Initiative for Asthma 2020 (GINA 2020). The results revealed that the majority (75.0%) of patients suffered from mild asthma. Patients treated with inhaled corticosteroids (ICS)/long-acting beta-agonists (LABA) at emergency department visits had lower frequencies of exacerbations compared with non-ICS/LABA-treated patients. The incidence rates for 1, 2, 3, and 4 exacerbation of the patients treated with ICS/LABA are lower than those treated without ICS/LABA (14.49 vs. 15.01%, 11.94% vs. 19.12%, 6.51% vs.12.92% and 4.10% vs. 9.35%). The difference got a statistical significance Chronic obstructive pulmonary disease (COPD) and gastroesophageal reflux disease (GERD), two comorbidities related to asthma, were risk factors for asthma exacerbation. Finally, patients who suffered from exacerbations produced a heavier economic burden compared to the patients who never suffered exacerbations (mean costs are ¥3,339.67 vs. ¥968.45 separately).  These results provide a reference for clinicians and patients to obtain a better treatment and therapy strategy management for people living with asthma.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 3","pages":"290-298"},"PeriodicalIF":1.5,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9974169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Health Status and Inconveniences of Asthmatic Children During the First Year of the COVID-19 Pandemic. 新冠肺炎大流行第一年哮喘患儿健康状况及不便之处
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-06-16 DOI: 10.18502/ijaai.v22i3.13052
Saber Gharagozlou, Negin Rostamzadeh, Mohammad Gharagozlou

The Coronavirus Disease 2019 (COVID-19) pandemic has been the most significant health concern in recent years, with respiratory symptoms being the most prominent. In children, asthma is the most prevalent chronic disease. Due to the similarities between the symptoms of these two conditions, we sought to assess the general health status of pediatric patients with asthma and their COVID-19-related difficulties during the first year of the pandemic in Iran. We collected data for this study by administering a questionnaire to the parents of 200 children with asthma who were registered in the database of the Asthma Clinic at a tertiary medical center in Tehran, Iran. Forty-five (22.5%) of 200 patients were suspected of having COVID-19, 11 (24.4%) underwent polymerase chain reaction (PCR) testing, and 10 (90.9%) assays were positive. During the first year of the pandemic, 41 patients (20.5%) were referred to a medical center at least once, with 31.7% due to an asthma attack. One hundred eighty-nine patients (94.5%) reported an improved disease status than the previous year, and only 31 patients (15.5%) were using asthma-related medications such as Salbutamol. The estimated mean Asthma Control Test (ACT) scores for two age categories, 4-11 years and 12-18 years, were 25.55±2.27 and 23.28±3.31, respectively, indicating satisfactory disease control. In the majority of our study population, asthma control was acceptable. However, the pandemic caused a significant increase in the anxiety levels of patients and their parents.

近年来,2019冠状病毒病(COVID-19)大流行一直是最严重的健康问题,其中呼吸道症状最为突出。在儿童中,哮喘是最普遍的慢性疾病。由于这两种情况的症状相似,我们试图评估伊朗大流行第一年儿童哮喘患者的总体健康状况及其与covid -19相关的困难。我们通过对200名哮喘儿童的父母进行问卷调查来收集本研究的数据,这些儿童在伊朗德黑兰一家三级医疗中心的哮喘诊所的数据库中登记。200例疑似病例45例(22.5%),PCR检测11例(24.4%),阳性10例(90.9%)。在大流行的第一年,41名患者(20.5%)被转诊到医疗中心至少一次,其中31.7%是由于哮喘发作。189名患者(94.5%)报告疾病状况较前一年有所改善,只有31名患者(15.5%)使用了与哮喘相关的药物,如沙丁胺醇。4-11岁和12-18岁两个年龄组的哮喘控制测试(ACT)估计平均得分分别为25.55±2.27和23.28±3.31,表明疾病控制满意。在我们的大多数研究人群中,哮喘控制是可以接受的。然而,大流行导致患者及其父母的焦虑程度显著增加。
{"title":"Health Status and Inconveniences of Asthmatic Children During the First Year of the COVID-19 Pandemic.","authors":"Saber Gharagozlou,&nbsp;Negin Rostamzadeh,&nbsp;Mohammad Gharagozlou","doi":"10.18502/ijaai.v22i3.13052","DOIUrl":"https://doi.org/10.18502/ijaai.v22i3.13052","url":null,"abstract":"<p><p>The Coronavirus Disease 2019 (COVID-19) pandemic has been the most significant health concern in recent years, with respiratory symptoms being the most prominent. In children, asthma is the most prevalent chronic disease. Due to the similarities between the symptoms of these two conditions, we sought to assess the general health status of pediatric patients with asthma and their COVID-19-related difficulties during the first year of the pandemic in Iran. We collected data for this study by administering a questionnaire to the parents of 200 children with asthma who were registered in the database of the Asthma Clinic at a tertiary medical center in Tehran, Iran. Forty-five (22.5%) of 200 patients were suspected of having COVID-19, 11 (24.4%) underwent polymerase chain reaction (PCR) testing, and 10 (90.9%) assays were positive. During the first year of the pandemic, 41 patients (20.5%) were referred to a medical center at least once, with 31.7% due to an asthma attack. One hundred eighty-nine patients (94.5%) reported an improved disease status than the previous year, and only 31 patients (15.5%) were using asthma-related medications such as Salbutamol. The estimated mean Asthma Control Test (ACT) scores for two age categories, 4-11 years and 12-18 years, were 25.55±2.27 and 23.28±3.31, respectively, indicating satisfactory disease control. In the majority of our study population, asthma control was acceptable. However, the pandemic caused a significant increase in the anxiety levels of patients and their parents.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 3","pages":"245-253"},"PeriodicalIF":1.5,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9902495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum Level of Antibodies Against Novel Acinetobacter Baumannii OmpA-selected Peptides in ICU Staff: Promise for the Future of Vaccine Development. ICU工作人员抗新型鲍曼不动杆菌ompa选择肽的血清抗体水平:疫苗开发的未来前景
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12676
Shadi Paydarfar, Mohammad Amin Abbasi, Ali Hashemi, Sodabe Taheri, Mojgan Bandehpour, Nariman Mosaffa

Extensively drug-resistant Acinetobacter baumannii is considered one of the most dangerous threats to global health, requiring novel therapeutic interventions. The outer membrane protein A (OmpA) is an immunogenic agent that triggers immune responses. The current study evaluated serum antibody levels against previously determined immunogenic OmpA peptides from A baumannii in ICU staff. Serum samples were collected from 62 ICU staff members (representing the exposed group), healthy controls (representing the nonexposed group), and patients with systemic lupus erythematosus (SLE) (as controls for nonspecific antibody reactions). After excluding the cross-reactive antibodies via Escherichia coli lysate pretreatment, all the samples were assessed in the vicinity of A baumannii lysate by enzyme-linked immunosorbent assay (ELISA). All the positive samples were assessed for interaction with previously designed and selected peptides using ELISA. The protective potential of positive serum antibodies was surveyed in vitro using an opsonophagocytic study. The most antibody positive samples against one of the dominant peptides were determined in the ICU personnel (75%).  SLE serum samples did not react with candidate peptides. The strongest positive reaction was observed in serum treatment with one of the OmpA peptides (No. 5) with significant differences compared to other designed peptides. Our findings showed that ICU samples have substantially higher antibody levels than the nonexposed group; Positive samples show strong results in the opsonophagocytosiis assay. This study demonstrates A baumannii colonization at human mucosal surfaces, especially in exposed healthy workers. Novel OmpA-derived peptides could be used to identify immunogenic vaccine candidates. Therefore, more studies are needed  before this peptide and antibody levels are used in diagnosis, prevention, or treatment.

广泛耐药鲍曼不动杆菌被认为是对全球健康最危险的威胁之一,需要新的治疗干预措施。外膜蛋白A (OmpA)是一种触发免疫反应的免疫原性因子。目前的研究评估了ICU工作人员针对先前确定的鲍曼杆菌免疫原性OmpA肽的血清抗体水平。从62名ICU工作人员(代表暴露组)、健康对照(代表未暴露组)和系统性红斑狼疮(SLE)患者(作为非特异性抗体反应的对照)中收集血清样本。经大肠杆菌裂解液预处理排除交叉反应性抗体后,采用酶联免疫吸附试验(ELISA)在鲍曼原虫裂解液附近进行评估。所有阳性样品均使用ELISA评估其与先前设计和选择的肽的相互作用。在体外用调理噬细胞法研究了阳性血清抗体的保护潜能。针对其中一种优势肽的抗体阳性样本最多的是ICU人员(75%)。SLE血清样本与候选肽没有反应。其中一种OmpA肽(No. 5)在血清处理中观察到最强的阳性反应,与其他设计的肽相比有显著差异。我们的研究结果显示,ICU样本的抗体水平明显高于未暴露组;阳性样品在调理噬噬试验中显示出很强的结果。本研究证实了鲍曼原虫在人体粘膜表面的定植,特别是在暴露的健康工人中。新的ompa衍生肽可用于鉴定免疫原性候选疫苗。因此,在将这种多肽和抗体水平用于诊断、预防或治疗之前,还需要进行更多的研究。
{"title":"Serum Level of Antibodies Against Novel Acinetobacter Baumannii OmpA-selected Peptides in ICU Staff: Promise for the Future of Vaccine Development.","authors":"Shadi Paydarfar,&nbsp;Mohammad Amin Abbasi,&nbsp;Ali Hashemi,&nbsp;Sodabe Taheri,&nbsp;Mojgan Bandehpour,&nbsp;Nariman Mosaffa","doi":"10.18502/ijaai.v22i2.12676","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12676","url":null,"abstract":"<p><p>Extensively drug-resistant Acinetobacter baumannii is considered one of the most dangerous threats to global health, requiring novel therapeutic interventions. The outer membrane protein A (OmpA) is an immunogenic agent that triggers immune responses. The current study evaluated serum antibody levels against previously determined immunogenic OmpA peptides from A baumannii in ICU staff. Serum samples were collected from 62 ICU staff members (representing the exposed group), healthy controls (representing the nonexposed group), and patients with systemic lupus erythematosus (SLE) (as controls for nonspecific antibody reactions). After excluding the cross-reactive antibodies via Escherichia coli lysate pretreatment, all the samples were assessed in the vicinity of A baumannii lysate by enzyme-linked immunosorbent assay (ELISA). All the positive samples were assessed for interaction with previously designed and selected peptides using ELISA. The protective potential of positive serum antibodies was surveyed in vitro using an opsonophagocytic study. The most antibody positive samples against one of the dominant peptides were determined in the ICU personnel (75%).  SLE serum samples did not react with candidate peptides. The strongest positive reaction was observed in serum treatment with one of the OmpA peptides (No. 5) with significant differences compared to other designed peptides. Our findings showed that ICU samples have substantially higher antibody levels than the nonexposed group; Positive samples show strong results in the opsonophagocytosiis assay. This study demonstrates A baumannii colonization at human mucosal surfaces, especially in exposed healthy workers. Novel OmpA-derived peptides could be used to identify immunogenic vaccine candidates. Therefore, more studies are needed  before this peptide and antibody levels are used in diagnosis, prevention, or treatment.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"150-162"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9879174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of Molecular-level Changes of Programmed Cell Death Ligand-1 after Radiation Therapy in a BALB/c CT26 Colorectal Mouse Tumor Model. BALB/c CT26结肠直肠癌小鼠肿瘤模型放射治疗后程序性细胞死亡配体-1分子水平变化的评价
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12677
Fateme Khani Chamani, Mahdi Shabani, Afshin Moradi, Maedeh Alinejad, Seyed Amir Jalali

The effects of radiation therapy (RT) for cancer can be systemic and partially mediated by the immune system. However, radiation alone is unlikely to transform an immunosuppressive environment into an immunostimulatory one. Therefore, an effective combination of RT and immunotherapy may provide a new, more efficient treatment approach. Here, we investigated how the expression of programmed cell death-ligand 1 (PD-L1) in the tumor microenvironment varied in different RT regimens with the same biologically effective dose. In this study, female BALB/c mice inoculated with CT26 tumor cells were irradiated with 3 different RT regimens using the same BED of 40 gray (Gy). These included ablative RT (1*15 Gy), hypo-fractionated RT (2*10 Gy), and conventional (Hyper-fractionated) RT (10*3 Gy). PD-L1 expression was analyzed with immunohistochemical staining on days 2 and 20 and when the size of tumors had reached 2 cm2 after RT. All treated groups expressed PD-L1, but the group receiving single ablative high-dose RT showed higher expression compared to the other groups. No significant differences in PD-L1 expression were observed at different times in the same group. These findings showed that different regimens of RT have different effects on the TME, so a combination of RT and immune checkpoint blockade could be clinically used in cancer patients.

放射治疗(RT)对癌症的影响可能是全身性的,部分由免疫系统介导。然而,单独的辐射不太可能将免疫抑制环境转变为免疫刺激环境。因此,RT和免疫治疗的有效结合可能提供一种新的、更有效的治疗方法。在这里,我们研究了在相同生物有效剂量的不同放射治疗方案中,肿瘤微环境中程序性细胞死亡配体1 (PD-L1)的表达是如何变化的。在本研究中,雌性BALB/c小鼠接种了CT26肿瘤细胞,使用相同的40 gray (Gy) BED,用3种不同的RT方案照射。其中包括消融放疗(1* 15gy)、低分流放疗(2* 10gy)和常规(超分流)放疗(10* 3gy)。免疫组织化学染色分析PD-L1在放疗后第2天、第20天及肿瘤大小达到2 cm2时的表达情况。所有治疗组均表达PD-L1,但单次消融大剂量放疗组的表达高于其他组。同一组不同时间PD-L1表达差异无统计学意义。这些发现表明,不同的放疗方案对TME有不同的影响,因此放疗与免疫检查点阻断联合应用可用于癌症患者的临床。
{"title":"Evaluation of Molecular-level Changes of Programmed Cell Death Ligand-1 after Radiation Therapy in a BALB/c CT26 Colorectal Mouse Tumor Model.","authors":"Fateme Khani Chamani,&nbsp;Mahdi Shabani,&nbsp;Afshin Moradi,&nbsp;Maedeh Alinejad,&nbsp;Seyed Amir Jalali","doi":"10.18502/ijaai.v22i2.12677","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12677","url":null,"abstract":"<p><p>The effects of radiation therapy (RT) for cancer can be systemic and partially mediated by the immune system. However, radiation alone is unlikely to transform an immunosuppressive environment into an immunostimulatory one. Therefore, an effective combination of RT and immunotherapy may provide a new, more efficient treatment approach. Here, we investigated how the expression of programmed cell death-ligand 1 (PD-L1) in the tumor microenvironment varied in different RT regimens with the same biologically effective dose. In this study, female BALB/c mice inoculated with CT26 tumor cells were irradiated with 3 different RT regimens using the same BED of 40 gray (Gy). These included ablative RT (1*15 Gy), hypo-fractionated RT (2*10 Gy), and conventional (Hyper-fractionated) RT (10*3 Gy). PD-L1 expression was analyzed with immunohistochemical staining on days 2 and 20 and when the size of tumors had reached 2 cm2 after RT. All treated groups expressed PD-L1, but the group receiving single ablative high-dose RT showed higher expression compared to the other groups. No significant differences in PD-L1 expression were observed at different times in the same group. These findings showed that different regimens of RT have different effects on the TME, so a combination of RT and immune checkpoint blockade could be clinically used in cancer patients.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"163-171"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9879175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insights into Overlappings of Fibrosis and Cancer: Exploring the Tumor-related Cardinal Genes in Idiopathic Pulmonary Fibrosis. 洞察纤维化和癌症的重叠:探索特发性肺纤维化中肿瘤相关的主要基因。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12680
Marjan Taherian, Paria Bayati, Mohammad-Ali Assarehzadegan, Mansoureh Soleimani, Hadi Poormoghim, Nazanin Mojtabavi

The pathogenesis of idiopathic pulmonary fibrosis (IPF) is quite similar to that of cancer pathogenesis, and several pathways appear to be involved in both disorders. The mammalian target of the rapamycin (mTOR) pathway harbors several established oncogenes and tumor suppressors. The same signaling molecules and growth factors, such as vascular endothelial growth factor (VEGF), contributing to cancer development and progression play a part in fibroblast proliferation, myofibroblast differentiation, and the production of extracellular matrix in IPF development as well. The expression of candidate genes acting upstream and downstream of mTORC1, as well as Vegf and low-density lipoprotein receptor related protein 1(Lrp1), was assessed using specific primers and quantitative polymerase chain reaction (qPCR) within the lung tissues of bleomycin (BLM)-induced IPF mouse models. Lung fibrosis was evaluated by histological examinations and hydroxyproline colorimetric assay. BLM-exposed mice developed lung injuries characterized by inflammatory manifestations and fibrotic features, along with higher levels of collagen and hydroxyproline. Gene expression analyses indicated a significant elevation of regulatory associated protein of mTOR (Raptor), Ras homolog enriched in brain (Rheb), S6 kinase 1, and Eukaryotic translation initiation factor 4E-binding protein 1 (4Ebp1), as well as a significant reduction of Vegfa, Tuberous sclerosis complex (Tsc2), and Lrp1; no changes were observed in the Tsc1 mRNA level. Our findings support the elevation of S6K1 and 4EBP1 in response to the TSC/RHEB/mTORC1 axis, which profoundly encourages the development and establishment of IPF and cancer. In addition, this study suggests a possible preventive role for VEGF-A and LRP1 in the development of IPF.

特发性肺纤维化(IPF)的发病机制与癌症的发病机制非常相似,两种疾病似乎都涉及几种途径。雷帕霉素(mTOR)途径的哺乳动物靶点包含几种已建立的癌基因和肿瘤抑制因子。同样的信号分子和生长因子,如血管内皮生长因子(VEGF),促进了癌症的发生和发展,也在成纤维细胞增殖、肌成纤维细胞分化和IPF发育中细胞外基质的产生中发挥了作用。在博来霉素(BLM)诱导的IPF小鼠模型肺组织中,使用特异性引物和定量聚合酶链反应(qPCR)评估mTORC1上游和下游的候选基因,以及Vegf和低密度脂蛋白受体相关蛋白1(Lrp1)的表达。采用组织学检查和羟脯氨酸比色法评价肺纤维化。暴露于blm的小鼠出现以炎症表现和纤维化特征为特征的肺损伤,同时胶原蛋白和羟脯氨酸水平升高。基因表达分析显示mTOR调控相关蛋白(Raptor)、脑富集Ras同源物(Rheb)、S6激酶1和真核翻译起始因子4e结合蛋白1 (4Ebp1)显著升高,Vegfa、结节性硬化症复合体(Tsc2)和Lrp1显著降低;Tsc1 mRNA水平未见明显变化。我们的研究结果支持S6K1和4EBP1的升高响应于TSC/RHEB/mTORC1轴,这深刻地促进了IPF和癌症的发展和建立。此外,本研究提示VEGF-A和LRP1在IPF的发生发展中可能具有预防作用。
{"title":"Insights into Overlappings of Fibrosis and Cancer: Exploring the Tumor-related Cardinal Genes in Idiopathic Pulmonary Fibrosis.","authors":"Marjan Taherian,&nbsp;Paria Bayati,&nbsp;Mohammad-Ali Assarehzadegan,&nbsp;Mansoureh Soleimani,&nbsp;Hadi Poormoghim,&nbsp;Nazanin Mojtabavi","doi":"10.18502/ijaai.v22i2.12680","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12680","url":null,"abstract":"<p><p>The pathogenesis of idiopathic pulmonary fibrosis (IPF) is quite similar to that of cancer pathogenesis, and several pathways appear to be involved in both disorders. The mammalian target of the rapamycin (mTOR) pathway harbors several established oncogenes and tumor suppressors. The same signaling molecules and growth factors, such as vascular endothelial growth factor (VEGF), contributing to cancer development and progression play a part in fibroblast proliferation, myofibroblast differentiation, and the production of extracellular matrix in IPF development as well. The expression of candidate genes acting upstream and downstream of mTORC1, as well as Vegf and low-density lipoprotein receptor related protein 1(Lrp1), was assessed using specific primers and quantitative polymerase chain reaction (qPCR) within the lung tissues of bleomycin (BLM)-induced IPF mouse models. Lung fibrosis was evaluated by histological examinations and hydroxyproline colorimetric assay. BLM-exposed mice developed lung injuries characterized by inflammatory manifestations and fibrotic features, along with higher levels of collagen and hydroxyproline. Gene expression analyses indicated a significant elevation of regulatory associated protein of mTOR (Raptor), Ras homolog enriched in brain (Rheb), S6 kinase 1, and Eukaryotic translation initiation factor 4E-binding protein 1 (4Ebp1), as well as a significant reduction of Vegfa, Tuberous sclerosis complex (Tsc2), and Lrp1; no changes were observed in the Tsc1 mRNA level. Our findings support the elevation of S6K1 and 4EBP1 in response to the TSC/RHEB/mTORC1 axis, which profoundly encourages the development and establishment of IPF and cancer. In addition, this study suggests a possible preventive role for VEGF-A and LRP1 in the development of IPF.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"190-199"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9872642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effects of Dupilumab for Asthma-chronic Obstructive Pulmonary Disease Overlap. Dupilumab对哮喘-慢性阻塞性肺疾病重叠的影响。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12683
Norio Kodaka, Chihiro Nakano, Takeshi Oshio, Takatomo Hirouchi, Yuka Yamada, Hiroto Matsuse

No Abstract.

没有抽象的。
{"title":"Effects of Dupilumab for Asthma-chronic Obstructive Pulmonary Disease Overlap.","authors":"Norio Kodaka,&nbsp;Chihiro Nakano,&nbsp;Takeshi Oshio,&nbsp;Takatomo Hirouchi,&nbsp;Yuka Yamada,&nbsp;Hiroto Matsuse","doi":"10.18502/ijaai.v22i2.12683","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12683","url":null,"abstract":"<p><p>No Abstract.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"212-216"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9872645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Inflammatory Markers in Patients with Depressed Episodes in Major Depressive Disorder and Bipolar Disorder before and after Treatment. 重度抑郁症和双相情感障碍抑郁发作患者治疗前后炎症标志物的评价。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12681
Laya Kafami, Sara Assadiasl, Sara Hashempour, Mohammad Hossein Niknam, Bahareh Mohebbi, Mohammad Amin Hashemnezhad, Bita Ansaripour, Arash Mirabzadeh, Banafsheh Mosharmovahed, Fateme Fazli, Mahshid Saleh, Nam I Mohammadian Khanssari, Ahmad Ali Noorbala

Depression is one of the current dilemmas in both developed and developing societies. Studies show that the severity of psychiatric symptoms is directly related to the degree of inflammation caused by cytokines secreted by the immune system. Hence, evaluating serum cytokine levels in patients with depression can help to understand the pathogenesis of the disease and make the best therapeutic decisions. The present study investigated the levels of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6) in patients with major depression or bipolar disorder during depressive episodes (BDDE) before and after a 6-month pharmaceutical intervention. Patients referring to 3 clinics were recruited for the study. The diagnosis of major depression or bipolar disorder in a depressive phase was made according to the Diagnostic and Statistical Manual of Mental Disorders -5(DSM-5) criteria. There was a significant difference in depression levels between the pre-intervention and 6-month follow-up in both groups. After 6 months, IL-1 and IL-6 levels in the bipolar disorder group had decreased while TNF-α levels had increased. There was also a significant difference between pre-intervention and follow-up levels of IL-1.  Serum levels of IL-1 and IL-6 decreased significantly in both groups after the 6-month follow-up, and symptom improvement was observed. TNF-α levels, on the other hand, decreased in the major depression group but increased in the bipolar disorder group. Considering that inflammation is a major outcome of depression, treatment strategies to reduce inflammation could be a practical approach to improving psychiatric symptoms.

抑郁症是当前发达社会和发展中社会共同面临的难题之一。研究表明,精神症状的严重程度与免疫系统分泌的细胞因子引起的炎症程度直接相关。因此,评估抑郁症患者的血清细胞因子水平可以帮助了解疾病的发病机制,并做出最佳的治疗决策。本研究调查了重度抑郁症或双相情感障碍患者在抑郁发作(BDDE)期间6个月药物干预前后的炎症因子、肿瘤坏死因子-α (TNF-α)、白细胞介素-1 (IL-1)和白细胞介素-6 (IL-6)水平。研究招募了3家诊所的患者。根据精神疾病诊断与统计手册-5(DSM-5)标准诊断抑郁症或抑郁期双相情感障碍。在干预前和6个月的随访中,两组的抑郁水平有显著差异。6个月后,双相情感障碍组IL-1和IL-6水平下降,TNF-α水平升高。干预前和随访期间IL-1水平也有显著差异。随访6个月后,两组患者血清IL-1、IL-6水平均显著下降,症状均有改善。另一方面,TNF-α水平在重度抑郁症组中下降,而在双相情感障碍组中升高。考虑到炎症是抑郁症的主要结果,减少炎症的治疗策略可能是改善精神症状的实用方法。
{"title":"Evaluation of Inflammatory Markers in Patients with Depressed Episodes in Major Depressive Disorder and Bipolar Disorder before and after Treatment.","authors":"Laya Kafami,&nbsp;Sara Assadiasl,&nbsp;Sara Hashempour,&nbsp;Mohammad Hossein Niknam,&nbsp;Bahareh Mohebbi,&nbsp;Mohammad Amin Hashemnezhad,&nbsp;Bita Ansaripour,&nbsp;Arash Mirabzadeh,&nbsp;Banafsheh Mosharmovahed,&nbsp;Fateme Fazli,&nbsp;Mahshid Saleh,&nbsp;Nam I Mohammadian Khanssari,&nbsp;Ahmad Ali Noorbala","doi":"10.18502/ijaai.v22i2.12681","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12681","url":null,"abstract":"<p><p>Depression is one of the current dilemmas in both developed and developing societies. Studies show that the severity of psychiatric symptoms is directly related to the degree of inflammation caused by cytokines secreted by the immune system. Hence, evaluating serum cytokine levels in patients with depression can help to understand the pathogenesis of the disease and make the best therapeutic decisions. The present study investigated the levels of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6) in patients with major depression or bipolar disorder during depressive episodes (BDDE) before and after a 6-month pharmaceutical intervention. Patients referring to 3 clinics were recruited for the study. The diagnosis of major depression or bipolar disorder in a depressive phase was made according to the Diagnostic and Statistical Manual of Mental Disorders -5(DSM-5) criteria. There was a significant difference in depression levels between the pre-intervention and 6-month follow-up in both groups. After 6 months, IL-1 and IL-6 levels in the bipolar disorder group had decreased while TNF-α levels had increased. There was also a significant difference between pre-intervention and follow-up levels of IL-1.  Serum levels of IL-1 and IL-6 decreased significantly in both groups after the 6-month follow-up, and symptom improvement was observed. TNF-α levels, on the other hand, decreased in the major depression group but increased in the bipolar disorder group. Considering that inflammation is a major outcome of depression, treatment strategies to reduce inflammation could be a practical approach to improving psychiatric symptoms.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"200-207"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9872646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Alleviating Impacts of Quercetin on Inflammation and Oxidant-antioxidant Imbalance in Rats with Allergic Asthma. 槲皮素对过敏性哮喘大鼠炎症及氧化-抗氧化失衡的缓解作用。
IF 1.5 4区 医学 Q4 ALLERGY Pub Date : 2023-04-30 DOI: 10.18502/ijaai.v22i2.12675
Mohammad Amin Rajizadeh, Mohammad Abbas Bejeshk, Amir Hossein Doustimotlagh, Hamid Najafipour, Maryam Eftekhari, Merat Mahmoodi, Mahdokht Azizi, Fahimeh Rostamabadi, Hossein Pourghadamyari

Asthma is an inflammatory disease of the airways. We assessed the anti-inflammatory and antioxidative impacts of quercetin, a plant derivative, on inflammatory and oxidative indices in lung tissue and serum of rats with asthma.Asth ma was induced by ovalbumin. Rats were divided into 4 groups: control, asthma+vehicle (Receieved normal saline), asthma+dexamethasone, and asthma+quercetin. After asthma induction, quercetin (50 mg/kg) and dexamethasone (2.5 mg/kg) were injected intraperitoneally once daily for 1 week. On day 50, lung histopathology indices; inflammatory factors; tissue gene expression, including GATA Binding Protein 3 (Gata-3), Tbx21 (T-bet), Transforming growth factor-β (TGF-β), Il10 (IL-10), Il1b (IL-1β), Il6 (IL-6), Acta2 (α-SMA), and Tnf (TNF-α); and oxidative stress indices (malondialdehyde [MDA], catalase [CAT], glutathione peroxidase [GPX], superoxide dismutase [SOD], and total antioxidant capacity [TAC]) in tissue and serum, were evaluated. The results showed that quercetin reduced Gata3, Tnf, Tgfb1, Il1b, and Acta2 gene expression and increased Tbx21 gene expression following asthma. Quercetin also improved oxidative stress by decreasing MDA levels and increasing TAC, CAT, SOD, and GPX levels in serum and lung tissue. Furthermore, quercetin decreased IL6 and TNFα levels and increased IL10 levels in lung tissue after asthma was treated with quercetin. Quercetin ameliorates oxidative stress and inflammation caused by asthma, especially at the tissue level. Therefore, quercetin can be considered a potent antiasthmatic agent.

哮喘是呼吸道的一种炎症性疾病。研究植物衍生物槲皮素对哮喘大鼠肺组织和血清炎症和氧化指标的抗炎和抗氧化作用。卵清蛋白诱导哮喘。将大鼠分为4组:对照组、哮喘+给药组(生理盐水)、哮喘+地塞米松组、哮喘+槲皮素组。哮喘诱导后,每日1次腹腔注射槲皮素(50 mg/kg)和地塞米松(2.5 mg/kg),连续1周。第50天,肺组织病理学指标;炎症因素;组织基因表达,包括GATA结合蛋白3 (GATA -3)、Tbx21 (T-bet)、转化生长因子-β (TGF-β)、IL-10 (IL-10)、il - 1b (IL-1β)、IL-6 (IL-6)、Acta2 (α-SMA)、Tnf (Tnf -α);测定组织和血清的氧化应激指标(丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)和总抗氧化能力(TAC))。结果显示,槲皮素可降低哮喘患者Gata3、Tnf、Tgfb1、Il1b和Acta2基因表达,增加Tbx21基因表达。槲皮素还通过降低血清和肺组织中MDA水平和增加TAC、CAT、SOD和GPX水平来改善氧化应激。槲皮素治疗哮喘后肺组织中il - 6、tnf - α水平降低,il - 10水平升高。槲皮素可改善哮喘引起的氧化应激和炎症,特别是在组织水平上。因此,槲皮素可以被认为是一种有效的平喘剂。
{"title":"The Alleviating Impacts of Quercetin on Inflammation and Oxidant-antioxidant Imbalance in Rats with Allergic Asthma.","authors":"Mohammad Amin Rajizadeh,&nbsp;Mohammad Abbas Bejeshk,&nbsp;Amir Hossein Doustimotlagh,&nbsp;Hamid Najafipour,&nbsp;Maryam Eftekhari,&nbsp;Merat Mahmoodi,&nbsp;Mahdokht Azizi,&nbsp;Fahimeh Rostamabadi,&nbsp;Hossein Pourghadamyari","doi":"10.18502/ijaai.v22i2.12675","DOIUrl":"https://doi.org/10.18502/ijaai.v22i2.12675","url":null,"abstract":"<p><p>Asthma is an inflammatory disease of the airways. We assessed the anti-inflammatory and antioxidative impacts of quercetin, a plant derivative, on inflammatory and oxidative indices in lung tissue and serum of rats with asthma.Asth ma was induced by ovalbumin. Rats were divided into 4 groups: control, asthma+vehicle (Receieved normal saline), asthma+dexamethasone, and asthma+quercetin. After asthma induction, quercetin (50 mg/kg) and dexamethasone (2.5 mg/kg) were injected intraperitoneally once daily for 1 week. On day 50, lung histopathology indices; inflammatory factors; tissue gene expression, including GATA Binding Protein 3 (Gata-3), Tbx21 (T-bet), Transforming growth factor-β (TGF-β), Il10 (IL-10), Il1b (IL-1β), Il6 (IL-6), Acta2 (α-SMA), and Tnf (TNF-α); and oxidative stress indices (malondialdehyde [MDA], catalase [CAT], glutathione peroxidase [GPX], superoxide dismutase [SOD], and total antioxidant capacity [TAC]) in tissue and serum, were evaluated. The results showed that quercetin reduced Gata3, Tnf, Tgfb1, Il1b, and Acta2 gene expression and increased Tbx21 gene expression following asthma. Quercetin also improved oxidative stress by decreasing MDA levels and increasing TAC, CAT, SOD, and GPX levels in serum and lung tissue. Furthermore, quercetin decreased IL6 and TNFα levels and increased IL10 levels in lung tissue after asthma was treated with quercetin. Quercetin ameliorates oxidative stress and inflammation caused by asthma, especially at the tissue level. Therefore, quercetin can be considered a potent antiasthmatic agent.</p>","PeriodicalId":14560,"journal":{"name":"Iranian journal of allergy, asthma, and immunology","volume":"22 2","pages":"138-149"},"PeriodicalIF":1.5,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9879176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
期刊
Iranian journal of allergy, asthma, and immunology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1