International Journal of Regenerative Medicine最新文献

{"title":"Autologous Micro Fragmented Adipose Cells Therapy for Subtalar Joint Osteoarthritis – Case Report and Review of Literature","authors":"N. Niazi, J. Wong, A. Pillai","doi":"10.31487/j.rgm.2022.01.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.01.01","url":null,"abstract":"Introduction: Post-traumatic subtalar joint arthritis is uncommon and the majority results as a consequence of either an intra-articular calcaneal or talar fracture. A unique case of management of subtalar joint arthritis is described using autologous microfragmented adipose cells therapy.\u0000Case Report: We presented a 48-year-old male with symptomatic left subtalar joint arthritis, which was managed conservatively with analgesia and multiple steroid injections. During surgery, fat cells were aspirated from the abdomen and transferred to the Lipogems kit for mechanical breakdown of adipose tissue for around 20 minutes. Final 10ml of the product was injected into the subtalar joint. Patient was followed up at regular intervals and Visual analogue score (VAS), Manchester-Oxford Foot Questionnaire (MOXFQ) and Foot and Ankle Ability Measure scores (FAM-ADL) were collected.\u0000Results: No perioperative complications were noted relating to abdominal liposuction and intra-articular injection of lipoaspirate and during the follow-up period. Improvement in VAS, MOX FQ and FAM-ADL scores was noted up to 6 months of follow-up. \u0000Discussion: The subtalar joint plays a central role in load transmission and movement at the hindfoot and its malalignment and arthritis lead to impaired function and pain. Intra articular injections of fat cells and their regenerative potential in degenerative joint diseases have been well documented in literature. Lipogem technique has been used in knee, hip and ankle arthritis, but it is not documented to use in the subtalar joint.\u0000Conclusion: This unique case report demonstrated the successful use of a single-dose autologous micro fragmented fat cells therapy leading to functional improvement in subtalar joint arthritis with no complications.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114577405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foreign Body Reaction with Granuloma 10 Years After Achilles Tendon Reconstruction with the LARS Ligament","authors":"N. Niazi, A. Aljawadi, M. Khan, A. Pillai","doi":"10.31487/j.rgm.2021.02.04","DOIUrl":"https://doi.org/10.31487/j.rgm.2021.02.04","url":null,"abstract":"Achilles tendon rupture is one of the most common tendon injury in adult population. Synthetic ligament has been an attractive alternative for reconstruction chronic Achilles tendon tears. Ligament reconstruction with the LARS ligament has been a popular choice owing to its low-complication rates. LARS ligament is a non-absorbable synthetic ligament device. There are no long-term results of LARS ligament reconstruction for Achilles tendon reconstruction in literature. We describe a successful management of foreign body granuloma in a LARS ligament Achilles tendon graft and a technique to reconstruct the tendon following its excision.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129052346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Harvesting and Processing Technique for Adipose Tissue Graft: Evaluation of Cell Viability","authors":"A. Gennai, B. Bovani, M. Colli, F. Melfa, D. Piccolo, R. Russo, B. Roda, A. Zattoni, P. Reschiglian, S. Zia","doi":"10.31487/j.rgm.2021.02.03","DOIUrl":"https://doi.org/10.31487/j.rgm.2021.02.03","url":null,"abstract":"Background: Clinical studies demonstrated the efficacy of therapies based on the autologous grafting of adult mesenchymal stem cells to accelerate the healing and regenerative processes of the skin and mesenchymal tissues; therefore, it is considered a valuable approach in the aesthetic rejuvenation treatment to give volumization and skin regeneration effects.\u0000Objective: The aim of the project consisted of the control of cell viability of adipose tissue (AT) harvested using the two types of cannulas having 0.8 mm and 1 mm side port holes. The results were compared with tissue harvested with a standard liposuction technique and processed with a standard procedure consisting of enzymatic digestion (collagenase).\u0000Methods: This study was performed on adipose tissues harvested from 7 patients (6 females and 1 male) with an average age of 48.5 years with 3 different techniques. We compared the cell vitality of every sample at T0 and T72.\u0000Results: Lipoaspirate tissue-derived by 0.8- and 1 mm cannula from all samples proved to be vital and possess viable cells. The average absorbance was similar immediately after plating (T0) and 72 hours after (T72) for the two cannulas, 0.8- and 1 mm cannula. The two systems proved to equally harvest vital tissue. An increase in cell viability was observed in all samples for each condition (0.8-, 1 mm and enzymatic digestion).\u0000Conclusion: This study proved that guided harvested adipose tissue with small cannulas with small side port holes yields a comparable amount of viable cells compared to adipose tissue harvested with a liposuction system and processed with enzymatic digestion (collagenase). This study confirms that the minimally invasive technique and minimal manipulation of the adipose tissue could yield a tissue with a good amount of viable cells. This micro fragmented adipose tissue is a promising source for regenerative treatments.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127063075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Number, Biophysical and Multipotent Characteristics of Adipose Derived Stem Cells Harvested by SEFFI Procedure and Interaction with Different Type of Hyaluronic Acids","authors":"A. Gennai, B. Bovani, M. Colli, F. Melfa, D. Piccolo, R. Russo, M. Tretti Clementoni, S. Zia, B. Roda, A. Zattoni","doi":"10.31487/j.rgm.2021.02.02","DOIUrl":"https://doi.org/10.31487/j.rgm.2021.02.02","url":null,"abstract":"Background: Injection of autologous adipose-derived stem cells (ADSCs) and a stromal vascular fraction (VSF) into dermal and subdermal layers promises regenerative advantages by improving skin volume and rejuvenation. Injectable hyaluronic acid (HA) is a temporary dermal filler that, by improving skin hydration, reduces the appearance of fine lines and wrinkles, facial folds and creates structure and volume to the face and lips. This study combined the grafting of micro fragmented fatty tissue with the hyaluronic acid filler procedure, using three different types of HA. \u0000Methods: Each sample of micro fragmented adipose tissue harvested using the superficial enhanced fluid fat injection (SEFFI) technique collected from 8 patients were equally divided into two specimens. One of these (EMU specimens) was emulsified by gently applying ten back-and-forth passages from one syringe to another to fluidify the tissue. The other one was not emulsified (Ctrl/NON-EMU specimen). Both EMU and NON-EMU specimens were divided into four aliquots: one served as control, and the others were combined with each of three tested hyaluronic acids. Afterward, we assessed the cellularity of mesenchymal phenotype (defined as the number of adherent cells with mesenchymal phenotype per milliliter of adipose tissue) and the in vitro capacity of differentiation in mesenchymal lineages.\u0000Results: Despite low cellularity from emulsified samples combined with HA, isolated cells could grow and expand in culture, thus proving their proliferative ability, showing “good quality” in all conditions (Ctrl/NON-EMU, EMU, and combined with HA). The cells could differentiate towards mesenchymal lineages, express mesenchymal markers by flow cytometry analysis, and maintain their stemness potential.\u0000Conclusion: The combination of emulsified harvested tissue with HA products can be exploited to counteract the loss of volume and skin aging of the human face and body. This approach to regenerative aesthetic treatment is a promising treatment for facial antiaging therapy.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121106196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Matrix Expression in Human Induced Pluripotent Stem Cell-Derived Optic Vesicles","authors":"H. Wang, Ramesh R. Kaini, Christina L. Rettinger","doi":"10.31487/j.rgm.2021.02.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2021.02.01","url":null,"abstract":"Background: Human tissue/organ development is a complex, highly orchestrated process, regulated in part by the surrounding extracellular matrix (ECM). Every complex tissue, including the retina, has a unique ECM configuration that plays a critical role in cellular differentiation, adhesion, migration, and maturation. \u0000Aim: To characterize ECM expression of human induced pluripotent stem cell-derived optic vesicles (iPSC-OVs). \u0000Methods: A 3- dimensional (3D) in vitro suspension culture system was used to direct differentiation of human induced pluripotent stem cells (iPSCs) into optic vesicles (OVs). Stepwise differentiation of iPSCs into retinal progenitor cells was confirmed by sequential expression of OTX2, SOX1, SIX6, LHX2, PAX6, and CHX10. Expression of ECM genes in iPSC-derived OVs was analyzed by RT2 ProfilerTM PCR Array, whereas immunofluorescence staining was performed to detect ECM proteins in the OVs. \u0000Results: A number of cell adhesion molecules (CAMs) previously reported to be abundantly expressed in iPSCs such as E-cadherin, Intercellular adhesion molecule-1 (ICAM1), Integrin-α L, Integrin-α M, Integrin-α 6 were downregulated while neural and retina specific CAMs including neural cell adhesion molecule 1 (NCAM1), neural plakophilin-related armadillo repeat protein (NPRAP), Integrin-α 1 and Integrin-α 4 were upregulated. Several glycoproteins that have been reported to play key roles during retinogenesis, namely CD44, Tenascin C, Tenascin R, Neurocan, Neuroglycan C, Delta 2 Catenin, Vitronectin, and Reelin were also present. \u0000Conclusion: We have identified an array of ECM proteins that were expressed during retinogenesis. Further characterization of these proteins will lead to a better understanding of retinal development.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134387645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infranatant Portion of Microfragmented Adipose Tissue: A Promising Source of SVF for the Management of Androgenetic Alopecia","authors":"A. Gennai, P. Tesauro, M. Colli, S. Zia, B. Roda, A. Zattoni","doi":"10.31487/J.RGM.2021.01.01","DOIUrl":"https://doi.org/10.31487/J.RGM.2021.01.01","url":null,"abstract":"Aim: The purpose of this article is to prove the possibility to transfer a good amount of cells of the SVF\u0000(and ADSCs) in the infranatant portion of microfragmented adipose tissue.\u0000Method: The adipose tissue harvesting procedure is performed under local anaesthesia. The adipose tissue\u0000was harvested with a 2 mm diameter microperforated cannula with 1 mm side port holes, mounted inside\u0000the special patented guide. Both cannula and guide are included in the SEFFIHAIR™ medical device. Once\u0000the adipose tissue is harvested, it is gently washed, and it was divided in two specimens: (EMU) the tissue\u0000was emulsified with 20 passages from one syringe to another and (CTRL) the tissue didn’t undergo any\u0000emulsification.\u0000Results: The emulsification procedure liberated alive and proliferating cells and we observed that the\u0000specimens derived with a 1 mm side port hole cannula and then emulsified (EMU) showed a higher number\u0000of cells in the infranatant part compared to the one derived from the control tissue without any (1 EMU vs.\u00001EMU infra).\u0000Conclusion: The use of microcannulas, in combination with a mechanical digestion by an emulsification\u0000procedure and centrifuge, could ease SVF cells isolation for regenerative treatment and could also be\u0000performed in a medical facility.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115272053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phase II Dose Escalation Study of Intraarterial (Hepatic) Adult Human Bone Marrow Derived, Cultured, Pooled, Allogeneic Mesenchymal Stromal Cells (Stempeucel®) in Patients with Alcoholic Liver Cirrhosis","authors":"Pawan Kumar Gupta, Anoop Chullikana, R. Seetharam, Udaykumar Kolkundar, P. ShivaShankar, Pachaiyappan Viswanathan, M. Chandrashekar, Charan Thej, K. Prasanth, Jijy Abraham, A. Majumdar","doi":"10.21203/RS.3.RS-182673/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-182673/V1","url":null,"abstract":"Background: Alcoholic liver cirrhosis is an end-stage alcoholic liver disease with a poor prognosis. The definitive treatment of alcoholic liver cirrhosis is orthotopic liver transplantation, which is expensive, requires long-term immunosuppression and is limited by the supply of organs. Being an unmet medical need, cell therapy is under investigation for alcoholic liver cirrhosis.\u0000Aims: This study was designed primarily for assessing the safety and feasibility of administering stempeucel® through the hepatic artery in alcoholic liver cirrhosis and secondarily to assess possible efficacy and dose-response.\u0000Methods: Sixty patients with alcoholic cirrhosis (18-65 years/Child-Pugh class B or C/Model for End-Stage Liver Disease score of minimum 10) were planned to be included in 6 groups: 2.5 million cells/kg Body Weight (2.5M Cell) and respective control (2.5M Control); 5 million cells/kg Body Weight (5M Cell) and respective control (5M Control); 7.5 million cells/kg Body Weight (5M Cell) and respective control (7.5M Control) with 10 patients in each group. Cell groups received stempeucel® administered via hepatic artery by catheterization through the femoral artery (Seldinger technique) and Standard Protocol of Care. The control group received Standard Protocol of Care. Patients were followed up at 1 week, 1 month, 3 months and 6 months. Efficacy evaluations included liver function test, Model for End-Stage Liver Disease score, Child-Pugh score, Short Form-36 questionnaire, liver stiffness using Fibroscan (Transient Elastography), and liver volume using Computerized Tomography scan.\u0000Results: Stempeucel® injection was well tolerated. Common treatment-emergent adverse events were gastrointestinal disorders, general disorders and administration site conditions and infections and infestations. Most of the treatment-emergent adverse events were unrelated / remotely related to stempeucel®. Thirty serious adverse events occurred in 10 patients (3 in 2.5M Cell, 5 in 5M Cell and one each in control groups). Three patients died due to SAEs: Two in 2.5M and one in 5M Cell group, none were related to stempeucel®. Statistically significant improvement was seen in 2.5M group compared to the control group in Short Form-36 score: bodily pain, mental component summary, vitality and social functioning.\u0000Conclusion: Stempeucel® was safe, well-tolerated and subjective improvement in Short Form-36 (bodily pain, mental component summary, vitality and social functioning and mental health) score was seen in the 2.5M cell group.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129204410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remdesivir for the Treatment of Severe SARS-CoV-2 (COVID-19): A Systematic Review and Meta-Analysis","authors":"Zhipeng Yan, K. Cheung, Eric Ho-Yin Lau, Ching‐lung Lai","doi":"10.31487/j.rgm.2020.04.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2020.04.01","url":null,"abstract":"Background: Coronavirus Disease in 2019 (COVID-19) is a pandemic caused by SARS-CoV-2 infection.\u0000Over 53 million people have been infected with over 1.3 million deaths. However, there is no standard\u0000treatment or vaccines to date. Recently, several randomized controlled trials and cohort studies have\u0000demonstrated the efficacy of remdesivir for the treatment of severe COVID-19 patients. This is a systematic\u0000review and meta-analysis to define its efficacy.\u0000Methods: A systematic review was done on databases (PubMed, Embase, Medline, Cochrane) on 9 Nov\u00002020. Search keywords were remdesivir, COVID-19, SARS-CoV-2, randomized controlled trials and cohort\u0000studies. Studies with high-evidence values were selected to evaluate its clinical efficacy in terms of risk\u0000ratio, time to clinical improvement, and mortality risk. Subgroup analysis was performed based on baseline\u0000hospitalization status, age and ethnicity.\u0000Results: Of the 1328 studies, 6 studies were selected and pooled for meta-analysis. Remdesivir was\u0000associated with clinical improvement (risk ratio 1.14, 95% CI 1.02-1.28, p=0.02). It shortened the mean\u0000time of clinical improvement by 3.32 days (95% CI -4.37 to -2.28, p<0.001). However, its use was not\u0000associated with reduced mortality risk (risk ratio 0.75, 95% CI 0.40–1.40). In subgroup analysis, remdesivir\u0000was associated with clinical improvement in patients without the need of invasive ventilation (risk ratio\u00001.90, 95% CI 1.58-2.29, p<0.001; hazard ratio 2.22, 95% CI, 1.64-3.02), and age less than 70 years (risk\u0000ratio 2.14, 95% CI 1.39-3.28, p<0.001).\u0000Conclusion: Remdesivir is effective in the treatment of severe COVID-19 patients, in particular those\u0000without invasive ventilation","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122486299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous Adipose-Derived Stem Cells, Platelet-Rich Plasma, and Fibrin Enhance Healing of Mandibular Bone Defects in Swine","authors":"M. Wheeler, Aaron J. Maki, A. C. M. Ercolin, J. J. Cooper, K. Roballo, M. Rubessa, R. Rabel","doi":"10.31487/j.rgm.2020.02.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2020.02.01","url":null,"abstract":"The primary aim of this study was to assess the therapeutic effects of autologous platelet-rich plasma and\u0000fibrin scaffolds combined with autologous adipose stem cells (ASCs) for critical-size defects in the pig\u0000mandible. Fibrin scaffolds supplemented with calcium hydrogen phosphate and platelet-derived growth\u0000factors were hypothesized to accelerate healing in porcine mandible bone compared to ASC-only injections\u0000and untreated controls. Three treatments included the use of autologous ASCs from liposuction with the\u0000addition of platelet-rich plasma, fibrin scaffolds, or as cell-only controls. All three treatments using ASCs\u0000were determined to increase bone mineral density and bone volume fraction compared to untreated controls.\u0000In general, the addition of both platelet-rich plasma and fibrin scaffolds to autologous ASCs from\u0000liposuction improved bone healing of critical-size defects.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116441546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Synergistic Effect of Anabolic Steroid and Loading on Intercellular Calcium Signaling Via Gap Junctions in Human Supraspinatus Tendon Cells","authors":"I. Triantafyllopoulos, N. Dede","doi":"10.31487/j.rgm.2020.01.05","DOIUrl":"https://doi.org/10.31487/j.rgm.2020.01.05","url":null,"abstract":"Objective: We hypothesized that anabolic steroid administration would act synergistically with substrate\u0000strain in two-dimensional cultures of human supraspinatus tendon cells, to upregulate the expression of\u0000connexin-43 and to increase the Ca2+ wave propagation through gap junctions.\u0000Methods: Supraspinatus tendon cells were isolated intra-operatively from human specimens during\u0000shoulder arthroscopy. Cells were plated in two-dimensional spot cultures and arranged into four\u0000experimental groups: 1) non-load, non-steroid (NLNS, n=12 wells); 2) non-load, steroid (NLS, n=12 wells);\u00003) load, non-steroid (LNS, n=12 wells); and 4) load, steroid (LS, n=12 wells) in order to produce bioartificial\u0000tendons (BATs). The load groups were stretched in culture plates and the steroid groups were given\u0000nandrolone decanoate. When BATs were macro- and microscopically mature, at five days, they were\u0000evaluated with immunocytochemistry for connexin-43 staining, fluorescence microscopy for calcium\u0000imaging and mechanical stimulation with a micropipette tip manipulation for calcium propagation. Dose\u0000response test was performed in order to establish any relation between nandrolone decanoate dose and\u0000calcium signaling response. ATP was applied to the spot culture cells from all groups and all patients to\u0000determine if the cells were sensitive to extracellular ATP.\u0000Results: Load-steroid group demonstrated the greatest density of cnx43 in comparison to all other groups.\u0000There were no significant differences between the groups considering the percentage of cells responding\u0000after mechanical stimulation (cell recruitment). The cells of load-steroid group showed a significantly\u0000greater mean peak [Ca2+]ic compared to the values of the other groups (p<0.05). The propagation time was\u0000significantly decreased in the LS group compared with the other groups (p<0.05). There were no significant\u0000differences between the groups considering the number of cells that were responding spontaneously prior\u0000to stimulation or the number of responding cells that were oscillating after the stimulation.\u0000Conclusion: Nandrolone decanoate and loading seem to have a synergistic effect on the upregulation of the\u0000gap junction protein cxn43 enhancing calcium signaling via gap junctions. Consecutively, anabolic steroid\u0000administration and load may enhance the formation of a better-organized cytoskeleton and particularly the\u0000actin stress monofilaments.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128924260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}