Journal of Animal Physiology and Animal Nutrition最新文献

This study was conducted to investigate the effect of diets with different metabolizable energy (ME) levels on broiler growth performance, nutrient digestibility, fecal score, organ weight and lesion score. A total of 1134 1-day-old Ross broiler chicks with an initial body weight (BW) of 44.33 ± 1.70 g were randomly assigned to three dietary treatment groups. There were 21 replicate pens per treatment, including 18 male birds per pen. The experimental period was divided into 3 phases and the diets were provided to broilers from days 1 to 34 of age: the starter (days 1-7), growing (days 8-20), and finishing (days 21-34). The dietary treatments included CON, basal diet (3200 Kcal/kg), TRT1, basal diet - 50 kcal/kg ME (3150 Kcal/kg), and TRT2, basal diet + 50 kcal/kg ME (3250 kcal/kg). The TRT2 group showed a reduced (p < 0.05) feed conversion ratio (FCR) compared to the TRT1 group during the growing phase. During the overall experimental period, TRT2 group broilers showed an increased (p < 0.05) average daily gain (ADG) and a reduced (p < 0.05) FCR than in the TRT1 group. However, there were no differences observed in feed intake (FI) and body weight gain (BWG), nutrient digestibility, fecal score, organ weight and lesion score. In conclusion, feeding a diet at 3250 kcal/kg ME from days 1-34 could be suitable for improving both average daily gain and feed conversion ratio in broiler chickens.

The present study aimed to investigate the effects of probiotic Bacillus licheniformis DSMZ 28710 supplementation on sow performance, Preweaning piglet performance, and the biochemical profiles of colostrum and milk. Sixty-eight crossbred sows (Landrace $\times$ Yorkshire) were allocated to either a standard lactation diet (Control; n = 35) or the Control diet supplemented with 10 g/sow/day of B. licheniformis DSMZ 28710 (Treatment; n = 33), from day 109 of gestation until day 21 of lactation. Sow and piglet performance, as well as the incidence of piglet diarrhea, were recorded. Moreover, the study investigated the changes in major chemical compositions, immunoglobulins, fatty acids, and non-volatile polar metabolites in colostrum, transient milk, and mature milk of sows. Supplementation of B. licheniformis increased piglet body weight on day 21 of lactation in old parity sows (p = 0.037). Moreover, the incidence of diarrhea was reduced in piglets suckled by sows supplemented with B. licheniformis DSMZ 28710, regardless of sow's parity or lactation stage (p < 0.05). Probiotic supplementation decreased fat content in transient milk (p = 0.026) and increased lactose content in mature milk (p = 0.011). Chemometric analysis revealed clear distinctions between the Control and Treatment group in the fatty acid profiles of colostrum, transient milk, and mature milk, while notable differences in non-volatile polar metabolite profiles were observed specifically in mature milk. In conclusion, supplementation with B. licheniformis DSMZ 28710 reduced the incidence of diarrhea in piglets, increased body weight of the piglets in old parity sows and altered the biomolecular profiles in colostrum, transit milk, and mature milk of the sows.

Ruminal methanogenesis reduces animal feed utilizing efficiency and is a potent greenhouse gas contributing to climate change. The present study aimed to study the effect of plant secondary metabolites on methane emission, nutrient digestibility and immune status of adult male goats. To fulfil the goal, 20 adult male goats (2.5-3 years of age, body weight [BW] 25.90 ± 1.46 kg) were divided into four groups of five animals. The control was fed a basal diet containing wheat straw and concentrate in a 50:50 ratio without additives. In groups T1, T2 and T3, the basal diet was supplemented with ajwain (Trachyspermum ammi) oil @ 0.06% dry matter intake (DMI), bahera (Terminalia belirica) @ 0.8% DMI and combination of ajwain oil and bahera @ 0.06% and 0.8% DMI, respectively. The DMI, nutrient digestibility, nitrogen intake, outgo and nitrogen balance were similar (p > 0.05) in all four groups. Methane emissions (g/kg DDMI) reduced (p < 0.05) by 22.16%, 23.88% and 33.49% in T1, T2 and T3 compared to control. Both cell-mediated immunity and humoral immune response significantly improved in T3 than other supplemented groups and control. In conclusion, the combination of behera and ajwain oil effectively reduced methane emissions and improved the immune status of adult male goats without affecting nutrient digestibility.

Bay laurel (Laurus nobilis) essential oil is known for its antimicrobial, anti-inflammatory, and antioxidant properties. This study examined the effects of L. nobilis oil (LN) on Nile tilapia (Oreochromis niloticus) under cold stress conditions (16°C). Tilapia (initial weight, 5.02 ± 0.02 g) were acclimatized to 16°C for 14 days before being fed diets containing 0, 0.5, 1.0, 1.5, 2.0, and 2.5 g/kg LN oil for 84 days. The 1.5 g/kg LN oil group exhibited the highest final body weight and weight gain (p ≤ 0.05), while survival rates peaked at 1 g/kg. Biometric indices and feed efficiency were significantly enhanced, particularly at 1.5 g/kg (p ≤ 0.05). Histological analysis revealed improved intestinal and hepatic structures in LN-supplemented groups, although mild alterations were observed at 2.0 and 2.5 g/kg. Blood biochemical analysis showed increased total protein and reduced cholesterol in supplemented groups. Immune responses, including serum lysozyme activity and bacterial inhibition, were significantly enhanced at 1.5 g/kg or higher (p ≤ 0.05). Antioxidant enzyme activities, including superoxide dismutase (SOD) and catalase (CAT), increased (p ≤ 0.05) with LN oil supplementation, while malondialdehyde (MDA) levels decreased, indicating reduced oxidative stress. Gene expression analysis demonstrated increased insulin-like growth factor 1 and glucose transporter 4 levels with 1.5 g/kg LN oil, and tumor necrosis factor-alpha expression decreased at higher dosages. Dietary LN oil, particularly at 1.5 g/kg, enhances growth, immunity, and antioxidant defense in Nile tilapia under cold stress. Future studies should optimize dosages and explore broader applications across species and conditions.

Thermoregulatory response and growth performance of FUNAAB-Alpha chickens administered sweet citrus peel powder (SCPP) were investigated in a twelve weeks experiment. A total of 360 1-day-old FUNAAB Alpha chicks were used for the study, comprising 120 birds per genotype: Normal feather (NF), Naked neck (NN) and Frizzle feather (FF). Birds were randomly allotted into 12 treatment groups: NF: 0 g, 3 g, 6 g and 9 g of SCPP, NN: 0 g, 3 g, 6 g and 9 g of SCPP, FF: 0 g, 3 g, 6 g and 9 g of SCPP. Each treatment was replicated thrice with 10 birds per replicate. No (p > 0.05) interactive and main effect of genotype and dosage of SCPP was observed on the Rectal temperature, Skin temperatures under wing and of the breast among the treatments. Similar (p > 0.05) interactive effect was observed on the respiratory rate (RR), however, higher (p < 0.001) RR was recorded in NN and FF compared with NF. Birds in the control group had the highest (p < 0.005) RR compared with birds administered 3 g and 9 g of SCPP/L of water. Interaction and main effect of SCPP dosage on heterophil (H), lymphocyte (L) and heterophil lymphocyte ratio (H/L) were not significant (p > 0.05) across the treatment groups. However, genotype significantly (p < 0.000) influenced H, L and H/L. The FF and NN had significantly (p < 0.000) lower H/L compared with the NF. Higher (p < 0.000) weight gain (WG) was observed in FF and NN compared to NF chickens. Significantly (p < 0.020) lower FCR was observed in 6 g/L (2.26), 3 g/L (2.65) and 9 g/L (2.66) of FF compared with NF on 9 g/L (4.03). Dosage of SCPP significantly (p < 0.001) influenced the relative weights of the intestine. In conclusion, lower H/L, higher WG and better FCR were observed in the NN and FF genotypes with the best FCR recorded at 6 g SCPP/L of water.

Bone damages in laying hens are of great concern in poultry farming. Besides various risk factors like housing systems or nutrient supply during egg production, it has often been hypothesized that genetically high-performing laying hens may be more prone to bone damages. The relevance of dietary support during the rearing period of pullets for optimal bone development has been little addressed so far. In the present study, an increasing dietary vitamin D₃ content within EU legislation was tested during the first 12 weeks of life in two high and two moderate-performing pullet lines (white and brown layer lines). For this purpose, a total of 940 chickens of both sexes were housed at the Institute of Animal Welfare and Animal Husbandry (Friedrich-Loeffler-Institut, Germany). The three experimental diets differed only regarding the added vitamin D₃ amount (300/1000/3000 IU Cholecalciferol/kg diet). After every 4 weeks, randomly chosen animals per genotype and dietary treatment were slaughtered for dissection. Serum 25(OH)-Vitamin D₃ concentrations reflected the dietary treatment. Body weight differed regarding genotype. No effect of dietary vitamin D₃ content as a single influence factor on bone parameters like breaking strength, bone dimensions or mineral content could be shown, but age, sex and genotype had impacts and influenced traits in an interactive manner. Therefore, during the first 12 weeks of the rearing period of layer pullets, the different dietary vitamin D₃ contents did not influence performance or bone parameters in the four genetically diverse purebred layer lines. Adjusted dietary vitamin D₃ recommendations for pullets depending on genetically predetermined egg-laying performance do not appear to be necessary if dietary vitamin D₃ contents are within EU legislation.

This study aimed to compare the effects of dietary supplementation of bacteriophage (BP) and acidifiers on performance, meat quality, morphology, and intestinal microbiota in chickens challenged and unchallenged with Salmonella enteritidis (SE) and also to investigate the possibility of replacing them in the diet with antibiotics. A total of 1760 male Ross (308) chicks were randomly assigned to 11 dietary treatments (8 pens/with 20 male chickens in each). Dietary treatments were as follows: SE-uninfected (negative control (NC), a basal diet without supplemention; NC+ 500 g/t BP (NBP1); NC+ 1000 g/t BP (NBP2); NC+ 300 mg/kg acidifier A (NAA); NC+ 300 mg/kg acidifier B (NAB)) and SE-infected (positive control (PC), a basal diet without supplemention; PC+ 40 mg/kg Antibiotic enrofloxacin (PA); PC+ 500 g/t BP (PBP1); PC+ 1000 g/t BP (PBP2); PC+ 3000 mg/kg acidifier A (PAA); PC+ 3000 mg/kg acidifier B (PAB)). At 13 D birds in challenged groups were orally gavaged with 1 mL bacterial suspension containing approximately 108 CFU Salmonella enterica. The results indicated that chicks challenged with SE impaired performance so that BWG and FI significantly decreased and FCR increased (p < 0.05). Diets containing a high dose of BP increased BW and improved FCR in challenged and unchallenged chickens. The quality of breast meat showed a decline in oxidation in chickens challenged with SE (p < 0.05). The inclusion of bacteriophage in the diet of chickens (1000 g/t) improved the L*, b*, and oxidation of meat. The use of bacteriophage and acidifier A in the diet significantly increased the Lactobacillus, and LAB count, especially in the challenged groups. The challenge of chickens with SE decreased the villus height and crypt depth in different parts of the small intestine (p < 0.05). The results of the present study suggested that 1000 g/t of BP probably improves chicken performance by increasing beneficial bacteria and decreasing pathogenic bacteria. Also, bacteriophage showed an improvement in the performance reduction of chickens with Salmonella infection. In addition, improved meat quality and beneficial changes in the microbiome and intestinal morphology indicate the effectiveness of BP as an alternative antibiotic growth promoter in broiler diets.

Tapirs are hindgut fermenters with a natural diet dominated by browse, with a certain proportion of wild fruit. By contrast, diets fed to tapirs in zoos are often dominated by domestic fruit and other sources of easily digestible carbohydrates, which have been linked to obesity and various health problems. We aimed at better understanding the digestive physiology of tapirs, measuring the digestive efficiency of 13 lowland (Tapirus terrestris) and five Malayan (Tapirus indicus) tapirs from five zoos on various zoo diets by recording intake and total faecal excretion. Additionally, we aimed at evaluating zoo diets, by assessing relationships between dietary characteristics and data on intake and faeces consistency, and compared the data gained in the zoo study to those of faecal samples of free-ranging tapirs. Tapirs showed digestive principles reported in other herbivores, such as higher levels of metabolic faecal nitrogen and a lower intake on diets of higher digestibility, or a decrease in digestibility with increasing dietary fibre concentrations. Compared to horses or grazing rhinoceroses, organic matter digestibility in tapirs was more negatively affected by dietary fibre; additionally, tapirs had comparatively low fibre digestibility. This is possibly linked to their comparatively poor chewing efficacy. The readiness to ingest a hay-only diet was directly proportional to the amount of hay in the usual zoo diet, supporting reports that it may be difficult to achieve high hay intakes in tapirs instantaneously. Faeces consistency was closer to the natural condition with higher proportions of roughage in the diet. Compared to a set of faecal samples from the wild, zoo faeces were generally lower in fibre. This study supports previous suggestions that zoo tapirs could benefit, in terms of mimicking their digestive physiology and feeding behaviour in natural habitats, from diets based on a readily ingested roughage source, especially from browse.

Insects are used as an alternative sustainable, protein-rich ingredient in fish, pet, pig and poultry diets. The significant difference between insect meals and common protein sources is the content of chitin. The nitrogen contained in chitin, which makes up 6.89% of the chitin mass, is detected as crude protein in the analysis and, therefore, deludes the crude protein content in a higher range. In this work, we developed a chitin analysis method that does not require expensive and specialized equipment within insect production and processing industries. The method is based on classical chemical methods such as crude fibre and nitrogen content, making it easily implementable within existing feed analysis. In the process of method validation, a recovery rate of over 95% for chitin in the presence of protein and a standard deviation of < 5% at concentrations as low as 2% was determined. Furthermore, determining chitin at a higher standard deviation of > 10% at concentrations as low as 2% is possible. The method was used to determine the chitin content in various products derived from insect breeding and processing. The chitin content was determined in four insect species (Hermetia Illucens; Tenebrio molitor; Acheta domesticus; Bombyx mori) and different developmental stages of the yellow mealworm (T. molitor), including larvae, pupae and beetles, as well as in commercial pet food. These results also allow for an estimation of the insect protein content, provided that the raw material is known.

This study aimed to determine the efficacy of red pine and larch pine needles powder at different doses on productive performance, egg quality, hatching parameters, total fatty acid and cholesterol levels of laying quails. A total of 126 (84 ♀ and 42 ♂) 20-week-old laying quail were randomly distributed into seven experimental diets with three replicates of six (four female and two male) birds per cage. The groups were fed a basal diet (CON group), a basal diet with red pine needle powder (RPNP group) (1.5%, 3% and 4.5%), or a basal diet with larch pine needle powder (LPNP group) (1.5%, 3% and 4.5%) for 9 weeks. The results showed that the additives which were added into the ration did not affect feed intake and feed conversion ratio among the performance parameters compared to the control group (p > 0.05). Egg production increased by 13%-14% in RPNP 1.5% and LPNP 4.5% groups compared to the control group (p < 0.05). Egg weight increased by 4.04% only at the RPNP 4.5% dose (p < 0.05). However, egg weight decreased by 3.68%, 2.88% and 5.75% in RPNP 3% and LPNP 3% and 4.5% doses, respectively (p < 0.05). The highest increase in egg mass compared to the control was 13.61% in the RPNP 1.5% dose (p < 0.05). Egg shape index increased by 8.18% in the RPNP 1.5% group and 11.11% in the LPNP 3% group (p < 0.05). Haugh units increased by 1.87% and 1.77% in RPNP 1.5% and LPNP 4.5% doses, respectively (p < 0.05). The amount of broken eggs increased in the LPNP 1.5% (2.86%) and LPNP 3% (3.65%) doses relative to the control group (0.40%) (p < 0.05). Egg yolk decreased by 7.2%-17.6% in RPNP 1.5% and 3% groups and LPNP 3% and 4.5% doses (p < 0.05). Egg yolk diameter decreased by 3.21%-6.56% in RPNP 1.5% and 3% groups and LPNP 4.5% doses (p < 0.05). Yolk index decreased by an average of 5.5%-7% in RPNP 1.5% and 4.5% doses and LPNP 4.5% doses (p < 0.05). Pine needle supplementation increased total monounsaturated fatty acids in egg yolks by 6% compared to the control group, while cholesterol levels decreased by 7.73% compared to the control (p < 0.05). On the other hand, pine needle addition had no effect on incubation parameters (p > 0.05). Our results clearly indicated that the 1.5% red pine needles powder could be used as an additive to increase the egg production, egg mass and Haugh units in laying quails.