首页 > 最新文献

Journal of Applied Biology & Biotechnology最新文献

英文 中文
Macrozoobenthos Community Structure in the Coastal Waters of Marsegu Island, Maluku, Indonesia 印度尼西亚马鲁古马赛岛沿海水域大型底栖动物群落结构
Pub Date : 2018-07-28 DOI: 10.20956/IJAB.V2I1.3224
F. Yunita, Fredy Leiwakabessy, D. Rumahlatu
The existence of macrozoobenthos in an ecosystem is closely related to the conditions of the surrounding environment. This research aimed to examine the physical chemical condition of aquatic environment and macrozoobenthos community structure in the coastal waters of Marsergu Island. This research was conducted in July and December 2016 in 3 stations. The data were collected by using sampling technique to measure the environmental factors (temperature, salinity, pH, and dissolved oxygen of seawater) and enumeration of gastropod species with in-situ. The results of this research showed that macrozoobenthos in the coastal waters of Marsegu Island consisted of 2 Phylum, namely Molusca and Echinoderms, that is, gastropod with 18 species, bivalvia with 5 species, and echinoderms with 17 species. Echinoderms was the dominant macrozoobentos with a percentage of 45%, followed by gastropods 42%, and bivalvia 13%. This suggests that echinoderms and gastropods are the main components that make up the macrozoobenthos community structure in the coastal waters of Marsegu Island. The results of the calculation of the diversity index of macrozoobenthos in the three research stations showed the highest score was station 1 as much as 3.56, station 2 as much as 3.52 and the lowest was station 3 as much as 2.07.
大型底栖动物在生态系统中的存在与周围环境条件密切相关。本研究旨在探讨马塞尔古岛沿海水域水环境的物理化学条件和大型底栖动物群落结构。本研究于2016年7月和12月在3个站点进行。数据采集采用采样法,实地测量环境因子(海水温度、盐度、pH、溶解氧)和腹足类动物计数。研究结果表明,马赛岛沿海海域大型底栖动物由软体动物和棘皮动物2门组成,其中腹足类18种,双壳类5种,棘皮动物17种。大型底栖动物以棘皮类为主,占45%,腹足类次之,占42%,双壳类占13%。这表明刺皮动物和腹足动物是构成马赛岛沿海大型底栖动物群落结构的主要组成部分。3个研究站大型底栖动物多样性指数计算结果显示,1号研究站最高,为3.56,2号研究站最高,为3.52,3号研究站最低,为2.07。
{"title":"Macrozoobenthos Community Structure in the Coastal Waters of Marsegu Island, Maluku, Indonesia","authors":"F. Yunita, Fredy Leiwakabessy, D. Rumahlatu","doi":"10.20956/IJAB.V2I1.3224","DOIUrl":"https://doi.org/10.20956/IJAB.V2I1.3224","url":null,"abstract":"The existence of macrozoobenthos in an ecosystem is closely related to the conditions of the surrounding environment. This research aimed to examine the physical chemical condition of aquatic environment and macrozoobenthos community structure in the coastal waters of Marsergu Island. This research was conducted in July and December 2016 in 3 stations. The data were collected by using sampling technique to measure the environmental factors (temperature, salinity, pH, and dissolved oxygen of seawater) and enumeration of gastropod species with in-situ. The results of this research showed that macrozoobenthos in the coastal waters of Marsegu Island consisted of 2 Phylum, namely Molusca and Echinoderms, that is, gastropod with 18 species, bivalvia with 5 species, and echinoderms with 17 species. Echinoderms was the dominant macrozoobentos with a percentage of 45%, followed by gastropods 42%, and bivalvia 13%. This suggests that echinoderms and gastropods are the main components that make up the macrozoobenthos community structure in the coastal waters of Marsegu Island. The results of the calculation of the diversity index of macrozoobenthos in the three research stations showed the highest score was station 1 as much as 3.56, station 2 as much as 3.52 and the lowest was station 3 as much as 2.07.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"33 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84800718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Biodiversity and distribution of gastropods at seagrass meadow of Balangdatu waters Tanakeke Island South Sulawesi Indonesia 印度尼西亚南苏拉威西岛Tanakeke岛Balangdatu水域海草草甸腹足类生物多样性及分布
Pub Date : 2017-12-31 DOI: 10.20956/IJAB.V1I2.3134
M. Litaay, Marwa Deviana, D. Priosambodo
The research about the biodiversity of gastropod has been conducted in seagrass meadow of Balangdatu waters, Tanakeke Island, South Sulawesi. The research aims to assess the diversity of gastropod species in Balangdatu waters. Sampling was conducted using quadrate transect method systematically. Three replicates of transect were applied for each station. The result indicates there were 34 species of gastropods from 14 genera and 14 families were found. Diversity index from every station varies from 1,661 to 2, 899. These values range from low to moderate. The diversity, Evenness, and dominance indices showed that Balangdatu waters still in good condition and sustain habitat for gastropod.
对南苏拉威西Tanakeke岛Balangdatu水域的海草草甸进行了腹足类生物多样性研究。本研究旨在评估巴郎达图水域腹足类物种的多样性。系统地采用方形样条法进行抽样。每个站点使用3个样条重复。结果表明,共发现腹足类动物14科14属34种。各电台的多样性指数从1661到2899不等。这些值的范围从低到中等。多样性指数、均匀度指数和优势度指数表明,巴郎达都水域仍处于良好的状态,维持着腹足动物的生存环境。
{"title":"Biodiversity and distribution of gastropods at seagrass meadow of Balangdatu waters Tanakeke Island South Sulawesi Indonesia","authors":"M. Litaay, Marwa Deviana, D. Priosambodo","doi":"10.20956/IJAB.V1I2.3134","DOIUrl":"https://doi.org/10.20956/IJAB.V1I2.3134","url":null,"abstract":"The research about the biodiversity of gastropod has been conducted in seagrass meadow of Balangdatu waters, Tanakeke Island, South Sulawesi. The research aims to assess the diversity of gastropod species in Balangdatu waters. Sampling was conducted using quadrate transect method systematically. Three replicates of transect were applied for each station. The result indicates there were 34 species of gastropods from 14 genera and 14 families were found. Diversity index from every station varies from 1,661 to 2, 899. These values range from low to moderate. The diversity, Evenness, and dominance indices showed that Balangdatu waters still in good condition and sustain habitat for gastropod.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"15 1","pages":"67-75"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83626801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Talinum paniculatum (Jacq) Gaertn (Java ginseng) production using Vesicular-Arbuscular Mycorrhizal 用囊状-丛枝菌根生产爪哇人参
Pub Date : 2017-12-31 DOI: 10.20956/IJAB.V1I2.3133
S. Sulistiono
Soil pathogen is one of main problem in Java ginseng production in tropical field. This study was aimed to evaluate the impact of VAM application to Java ginseng tuber production. Application of VAM was conducted in various concentrations (0, 1, 2, and 3%) (w/w). Thin Layer Chromatography was used to detected saponin and spectrophotometry was used to determine total saponin. Biomass, the number of rotten tuber, and total saponin were determined after eleven months under greenhouse condition. Inoculation 3% of VAM can enhance the number of healthy and biomass Java ginseng tuber significantly. But the application of VAM did not increase total saponin content.
土壤病原菌是热带地区爪哇人参生产中的主要问题之一。本研究旨在评价VAM对爪哇人参块茎生产的影响。分别以不同浓度(0、1、2和3%)(w/w)施用VAM。用薄层色谱法测定总皂苷,用分光光度法测定总皂苷。在温室条件下测定11个月后的生物量、腐烂块茎数和总皂苷。接种3%的VAM可显著提高爪哇人参健康块茎的数量和生物量。但VAM的施用并没有提高总皂苷的含量。
{"title":"Talinum paniculatum (Jacq) Gaertn (Java ginseng) production using Vesicular-Arbuscular Mycorrhizal","authors":"S. Sulistiono","doi":"10.20956/IJAB.V1I2.3133","DOIUrl":"https://doi.org/10.20956/IJAB.V1I2.3133","url":null,"abstract":"Soil pathogen is one of main problem in Java ginseng production in tropical field. This study was aimed to evaluate the impact of VAM application to Java ginseng tuber production. Application of VAM was conducted in various concentrations (0, 1, 2, and 3%) (w/w). Thin Layer Chromatography was used to detected saponin and spectrophotometry was used to determine total saponin. Biomass, the number of rotten tuber, and total saponin were determined after eleven months under greenhouse condition. Inoculation 3% of VAM can enhance the number of healthy and biomass Java ginseng tuber significantly. But the application of VAM did not increase total saponin content.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"153 1","pages":"76-81"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86787580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Study of Lacid Acid Bacteria Activities from Human Oral Mucosa for Candida albicans Inhibition 人口腔黏膜酸菌活性对白色念珠菌抑制作用的研究
Pub Date : 2017-12-30 DOI: 10.20956/IJAB.V1I2.2261
Evi Lauw
Lactic acid bacteria are known to have potential in producing antimicrobial compounds, including antifungal agents. This study aims to determine whether the lactic acid bacteria from human oral mucosa have the potential to produce the secondary metabolite to inhibit the candidiasis, which caused by pathogen fungi, Candida albicans. Lactic acid bacteria were isolated from three healthy respondents based on age difference that is an infant, child, and adult. Lactic acid bacteria isolation using the deMann Rogosa Sharpe Broth (MRSB)-liquid medium, followed by purification stage with the quadrant scratch method using the deMann Rogosa Sharpe Agar (MRSA)-solid medium. The purified isolates obtained were characterized and identified by Gram staining, biochemical tests, and inhibition zone diameter measurement toward pathogenic fungi Candida albicans. The results were obtained from each of the two lactic acid bacteria isolates from each respondent with characteristics including gram-positive bacteria with short stem cell shape, possibly as Lactobacillus sp. It is conclude that the lactic acid bacteria of oral mucosa from adult had greater anticandidal activity than infant and child. Keywords: lactic acid bacteria, human oral mucosa, antifungal activity, Candida albicans
众所周知,乳酸菌在生产抗微生物化合物,包括抗真菌剂方面具有潜力。本研究旨在确定人类口腔黏膜乳酸菌是否具有产生次生代谢物抑制念珠菌病的潜力,念珠菌病是由白色念珠菌引起的。乳酸菌从三个健康的调查对象中分离出来,根据年龄的差异,分别是婴儿、儿童和成人。乳酸菌分离采用deMann Rogosa Sharpe Broth (MRSB)-液体培养基,然后采用deMann Rogosa Sharpe Agar (MRSA)-固体培养基采用象限划痕法纯化阶段。通过革兰氏染色、生化试验和对白色念珠菌的抑菌带直径测定对分离物进行了鉴定。从每个应答者身上分离的两株乳酸菌均具有革兰氏阳性菌的特征,具有短干细胞形状,可能为乳杆菌属。结果表明,成人口腔黏膜乳酸菌的抗药活性高于婴儿和儿童。关键词:乳酸菌,人口腔黏膜,抗真菌活性,白色念珠菌
{"title":"Study of Lacid Acid Bacteria Activities from Human Oral Mucosa for Candida albicans Inhibition","authors":"Evi Lauw","doi":"10.20956/IJAB.V1I2.2261","DOIUrl":"https://doi.org/10.20956/IJAB.V1I2.2261","url":null,"abstract":"Lactic acid bacteria are known to have potential in producing antimicrobial compounds, including antifungal agents. This study aims to determine whether the lactic acid bacteria from human oral mucosa have the potential to produce the secondary metabolite to inhibit the candidiasis, which caused by pathogen fungi, Candida albicans. Lactic acid bacteria were isolated from three healthy respondents based on age difference that is an infant, child, and adult. Lactic acid bacteria isolation using the deMann Rogosa Sharpe Broth (MRSB)-liquid medium, followed by purification stage with the quadrant scratch method using the deMann Rogosa Sharpe Agar (MRSA)-solid medium. The purified isolates obtained were characterized and identified by Gram staining, biochemical tests, and inhibition zone diameter measurement toward pathogenic fungi Candida albicans. The results were obtained from each of the two lactic acid bacteria isolates from each respondent with characteristics including gram-positive bacteria with short stem cell shape, possibly as Lactobacillus sp. It is conclude that the lactic acid bacteria of oral mucosa from adult had greater anticandidal activity than infant and child. Keywords: lactic acid bacteria, human oral mucosa, antifungal activity, Candida albicans","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"26 1","pages":"58-66"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86865645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Yeast Based Biofertilizer combined with bacteria on Mustard Plant Growth 酵母基生物肥料配菌对芥菜生长的影响
Pub Date : 2017-12-29 DOI: 10.20956/IJAB.V1I2.3093
N. Alami
Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard ( Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers. Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant
生物肥料通常被定义为微生物接种剂,它能够通过其生物过程将土壤中的重要营养物质从非有效形式转移到植物的有效形式。因此,为了提高土壤的生产力,使用生物肥料是必要的。念珠菌作为磷酸盐增溶剂和分解者。固氮细菌作为固氮菌,芽孢杆菌作为磷酸盐增溶剂。这些微生物作为生物肥料组合使用对芥菜(Brassica juncea L.)植株生长的影响有待研究。结果表明,配施念珠芽孢杆菌的产量最高,株高13.6 cm,湿重4.96 g,干重2.29 g。假丝酵母偶氮菌组合对叶片宽度和叶片数的影响最大,分别为20.3 cm和5.3 cm。固氮菌组合在根长参数上产量最高,为5.0 cm。生物肥料的添加有望减少化肥残留对土壤的污染。关键词:固氮菌,芽孢杆菌,生物肥料,念珠菌,芥菜
{"title":"Effect of Yeast Based Biofertilizer combined with bacteria on Mustard Plant Growth","authors":"N. Alami","doi":"10.20956/IJAB.V1I2.3093","DOIUrl":"https://doi.org/10.20956/IJAB.V1I2.3093","url":null,"abstract":"Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard ( Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers. Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"11 1","pages":"46-57"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89285225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus 高效液相色谱法分析低地柑橘橘色积累过程中β-隐黄质和玉米黄质含量
Pub Date : 2017-12-29 DOI: 10.20956/IJAB.V1I2.3066
Inanpi Hidayati Sumiasih, R. Poerwanto, D. Efendi, A. Agusta, S. Yuliani
Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine . It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin . The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits. Keyword : β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin .
柑橘果皮颜色是柑橘果皮的主要品质特征之一,是类胡萝卜素及其衍生物,尤其是β-柠檬酸苷积累的结果。它使柑橘皮的颜色看起来很吸引人(橙色)。橙色是β-隐黄质和β-柑橘素的混合物。本研究的目的是:(1)观察预冷和适当乙烯暴露时间对柑桔果皮橙色形成的影响;(2)鉴定和测定柑桔果皮β-隐黄质含量和总叶绿素含量。柑橘产自东爪哇图班,研究在PKHT IPB和LIPI进行。在注射100 ppm暴露于15°C的乙烯之前进行预冷和不进行预冷处理,暴露控制持续时间(0),24和48小时。结果表明,柑桔色指数(CCI)的最佳颜色为预冷处理和乙烯处理24h,可使柑桔果皮总叶绿素含量降低约8倍,β-隐黄质色素含量提高5倍,加速柑桔果皮颜色向鲜橙色的形成。脱脂对柑桔果实的总溶解固形物和硬度无显著影响。关键词:β-隐黄菌素;柑橘;叶绿素;degreening;乙烯玉米黄质。
{"title":"The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus","authors":"Inanpi Hidayati Sumiasih, R. Poerwanto, D. Efendi, A. Agusta, S. Yuliani","doi":"10.20956/IJAB.V1I2.3066","DOIUrl":"https://doi.org/10.20956/IJAB.V1I2.3066","url":null,"abstract":"Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine . It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin . The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits. Keyword : β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin .","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"99 1","pages":"37-45"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80575008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System 蚯蚓(Lumbricus rubellus)提取物在体外给药系统中的包封效率研究
Pub Date : 2017-08-18 DOI: 10.20956/IJAB.V1I1.2269
Sabrina Resky Pratiwi, Nur Ainiah, Hardyanti Hardyanti, Dini Rusdayanti Putri, Emilia Utomo
Introduction Earthworms (Lumbricus rubellus) extract is known to contain  bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome.  Methods Earthworms powder was macerated using 50% ethanol for 3 days. The extract was  formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible. Results The results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol. Conclusions The highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%. Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.
蚯蚓(Lumbricus rubellus)提取物含有生物活性蛋白作为抗菌化合物。极性化合物的缺点之一是渗透到皮肤层的速度慢,这可以通过将其配制成溶酶体给药系统来解决。本研究的目的是获得乙醇浓度的信息,可以使酶体的最高包封效率。方法用50%乙醇浸泡蚯蚓粉3 d。将提取液配制成乙醇浓度分别为20%、30%、40%和50%的乙醇体。采用紫外可见分光光度计测定蚯蚓提取物在溶酶体囊泡中的活性蛋白含量,从而测定其包封效率。结果以0.5%的提取液、2%的大豆卵磷脂和50%的乙醇为最佳配比,其提取率为72.58%。结论蚯蚓提取液溶酶体包封率最高,为72.58%。关键词:乙醇体;乙醇浓度;捕集效率;
{"title":"A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System","authors":"Sabrina Resky Pratiwi, Nur Ainiah, Hardyanti Hardyanti, Dini Rusdayanti Putri, Emilia Utomo","doi":"10.20956/IJAB.V1I1.2269","DOIUrl":"https://doi.org/10.20956/IJAB.V1I1.2269","url":null,"abstract":"Introduction Earthworms (Lumbricus rubellus) extract is known to contain  bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome.  Methods Earthworms powder was macerated using 50% ethanol for 3 days. The extract was  formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible. Results The results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol. Conclusions The highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%. Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"62 1","pages":"32-36"},"PeriodicalIF":0.0,"publicationDate":"2017-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84495237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Production of Chitosan from Shrimp Shell Waste and Its Formulation in Patch DosageForm Combined with Aloe vera Extract as Antiinfection Agent 虾壳废弃物制备壳聚糖及其贴片剂型与芦荟提取物联合抗感染的研究
Pub Date : 2017-08-18 DOI: 10.20956/IJAB.V1I1.2277
Haeriah Haeriah, M. Rahmatullah, Andi Indardaya, Emilia Utomo, N. Novianti, S. Sartini
Chitosan can be obtained from chitin isolated from shrimp waste through the process of deacetylation of chitin. Chitosan can be used as a patch base material because of its biocompatibility and biodegradability, and has antibacterial activity. The combination of Chitosan and Aloe vera extracts may be useful in patch dosage forms as wound dressings that have antiinfective activity. The research methods was laboratory experimental which consists of : chitosan was obtained from shell waste sequentially by deproteinisation, demineralisation, and deacetylation processes, and analyzed its characteristic, respectivel; Aloe vera gel was extraction by maceration methods using ethanol as solvent; patches were formulated using 2 % chitosan in 1.5 % glacial acetic acid,  1.6 %  Aloe vera extracts and 10 % glycerin and evaluated its physical propoperties,  skin iritation test, and antibacterial test against S.aureus. The results showed that percentage yield of chitosan was 32.67%, with characteristics; Its viscosity 93.33 cps, pH 4, 30 % deacetylation degree.  Percentage yield of Aloe vera  extract was 22.73%, containing acemannan, and had antibacterial activity against S.aureus .  The result of patch evaluation showed that the thickness of patch was 0.45 mm, 114 times folding endurance, moisture uptake of 45.03%, moisture content of 23.64%, safe to use, and  diameter of inhibition zone was 6.39 mm against S.aureus. So, combination of Aloe vera and Chitosan from shrimp shell as patch may use as antiinfection wound healing. Keywords : Aloe vera L.; chitosan; wound dressing; patch; shrimp shell waste
从虾废中分离甲壳素,经甲壳素脱乙酰处理可制得壳聚糖。壳聚糖具有良好的生物相容性和生物可降解性,并具有抗菌活性,可作为贴片基材料。壳聚糖和芦荟提取物的组合可能是有用的贴片剂型伤口敷料,具有抗感染活性。采用室内实验的方法,对壳类废弃物进行脱蛋白、脱矿、脱乙酰等工艺制备壳聚糖,并对其特性进行了分析;以乙醇为溶剂,采用浸渍法提取芦荟凝胶;以2%壳聚糖、1.5%冰醋酸、1.6%芦荟提取物和10%甘油配制贴剂,并对其进行物理性能、皮肤刺激试验和金黄色葡萄球菌抑菌试验。结果表明,壳聚糖得率为32.67%,具有一定的特点;其粘度93.33 cps, pH值4,脱乙酰度30%。芦荟提取物提取率为22.73%,含有葡甘露聚糖,对金黄色葡萄球菌具有抑菌活性。膜片评价结果表明,膜片厚度为0.45 mm,可折叠114次,吸湿率为45.03%,含水率为23.64%,可安全使用,对金黄色葡萄球菌的抑菌带直径为6.39 mm。因此,芦荟和虾壳壳聚糖的组合作为贴剂可以用于抗感染伤口愈合。关键词:芦荟;壳聚糖;伤口敷料;补丁;虾壳废料
{"title":"The Production of Chitosan from Shrimp Shell Waste and Its Formulation in Patch DosageForm Combined with Aloe vera Extract as Antiinfection Agent","authors":"Haeriah Haeriah, M. Rahmatullah, Andi Indardaya, Emilia Utomo, N. Novianti, S. Sartini","doi":"10.20956/IJAB.V1I1.2277","DOIUrl":"https://doi.org/10.20956/IJAB.V1I1.2277","url":null,"abstract":"Chitosan can be obtained from chitin isolated from shrimp waste through the process of deacetylation of chitin. Chitosan can be used as a patch base material because of its biocompatibility and biodegradability, and has antibacterial activity. The combination of Chitosan and Aloe vera extracts may be useful in patch dosage forms as wound dressings that have antiinfective activity. The research methods was laboratory experimental which consists of : chitosan was obtained from shell waste sequentially by deproteinisation, demineralisation, and deacetylation processes, and analyzed its characteristic, respectivel; Aloe vera gel was extraction by maceration methods using ethanol as solvent; patches were formulated using 2 % chitosan in 1.5 % glacial acetic acid,  1.6 %  Aloe vera extracts and 10 % glycerin and evaluated its physical propoperties,  skin iritation test, and antibacterial test against S.aureus. The results showed that percentage yield of chitosan was 32.67%, with characteristics; Its viscosity 93.33 cps, pH 4, 30 % deacetylation degree.  Percentage yield of Aloe vera  extract was 22.73%, containing acemannan, and had antibacterial activity against S.aureus .  The result of patch evaluation showed that the thickness of patch was 0.45 mm, 114 times folding endurance, moisture uptake of 45.03%, moisture content of 23.64%, safe to use, and  diameter of inhibition zone was 6.39 mm against S.aureus. So, combination of Aloe vera and Chitosan from shrimp shell as patch may use as antiinfection wound healing. Keywords : Aloe vera L.; chitosan; wound dressing; patch; shrimp shell waste","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"38 1","pages":"22-31"},"PeriodicalIF":0.0,"publicationDate":"2017-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85107445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Diversity of Invertebrate Epifaunas Associating with Mangrove In Balangdatu Village Tanakeke Islands of South Sulawesi 南苏拉威西岛Tanakeke群岛Balangdatu村红树林伴生无脊椎动物的多样性
Pub Date : 2017-07-22 DOI: 10.20956/IJAB.V1I1.2104
Crisnawati Crisnawati, M. Litaay, Dody Prisambodo, Slamet Santoso
Research on the diversity of invertebrate epifaunas associating with mangroves in the Balangdatu village of Tanakeke regency, Takalar district, South Sulawesi, has been conducted from September to December 2016. The objective of this research was to know the diversity of invertebrate epifaunas associating with mangrove in Balangdatu Village of Tanakeke Islands. Sample collection was done by using explorative quantitatively plot methods with size 2 x 2 m, applied at three different stations. Data analysis includes calculation of Absolute density, Uniformity Index (Shannon-wiener), Dominance Index, and Distribution Index. The results showed that there were 13 species of epi fauna of 9 tribes, namely Littoraria sp 1, Cerithidea cingulata , Littoraria sp 2, Littoraria pallescens , Episesarma sp , Saccostrea cucullata , Pagurus sp, Terebralia sulcata , Cerithidea sp , Nerita planospira , Nisto histrio , Littoraria scabra , and Cassidula vespertilionis . The highest density was found in Littoraria scabra with a density of 0.72 ind/m 2 . Diversity indices are low which indicate depressed environmental conditions. Distribution Index values across stations <1 indicating that the pattern of individual dispersion tends to be uniform.
2016年9月至12月,在南苏拉威西塔卡拉区Tanakeke regency的Balangdatu村对与红树林相关的无脊椎动物表层动物的多样性进行了研究。本研究的目的是了解田纳克群岛巴朗达图村红树林伴生无脊椎动物的多样性。样品采集采用探索性定量图法,尺寸为2 × 2 m,应用于三个不同的站点。数据分析包括绝对密度、均匀性指数(Shannon-wiener)、优势度指数和分布指数的计算。结果表明,该地区有9个部落13种外栖动物,分别为Littoraria sp 1、Cerithidea cingulata、Littoraria sp 2、Littoraria pallescens、Episesarma sp、Saccostrea cucullata、Pagurus sp、Terebralia sulcata、Cerithidea sp、Nerita planospira、Nisto hio、Littoraria scabra和Cassidula vespertilionis。其密度以Littoraria scabra最高,为0.72 ind/ m2。多样性指数较低,表明环境条件较差。站间分布指数<1,表明个体分散格局趋于均匀。
{"title":"Diversity of Invertebrate Epifaunas Associating with Mangrove In Balangdatu Village Tanakeke Islands of South Sulawesi","authors":"Crisnawati Crisnawati, M. Litaay, Dody Prisambodo, Slamet Santoso","doi":"10.20956/IJAB.V1I1.2104","DOIUrl":"https://doi.org/10.20956/IJAB.V1I1.2104","url":null,"abstract":"Research on the diversity of invertebrate epifaunas associating with mangroves in the Balangdatu village of Tanakeke regency, Takalar district, South Sulawesi, has been conducted from September to December 2016. The objective of this research was to know the diversity of invertebrate epifaunas associating with mangrove in Balangdatu Village of Tanakeke Islands. Sample collection was done by using explorative quantitatively plot methods with size 2 x 2 m, applied at three different stations. Data analysis includes calculation of Absolute density, Uniformity Index (Shannon-wiener), Dominance Index, and Distribution Index. The results showed that there were 13 species of epi fauna of 9 tribes, namely Littoraria sp 1, Cerithidea cingulata , Littoraria sp 2, Littoraria pallescens , Episesarma sp , Saccostrea cucullata , Pagurus sp, Terebralia sulcata , Cerithidea sp , Nerita planospira , Nisto histrio , Littoraria scabra , and Cassidula vespertilionis . The highest density was found in Littoraria scabra with a density of 0.72 ind/m 2 . Diversity indices are low which indicate depressed environmental conditions. Distribution Index values across stations <1 indicating that the pattern of individual dispersion tends to be uniform.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"1 1","pages":"14-21"},"PeriodicalIF":0.0,"publicationDate":"2017-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89257166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Biofiltration Efficiency of Algae Spirogyra hyalina to Reduce Salinity of Sea Water 透明丝绵藻降低海水盐度的生物过滤效果
Pub Date : 2017-07-10 DOI: 10.20956/IJAB.V1I1.2003
A. Putra
Algae Spyrogyra hyalina is one species of fresh water algae that is very easy to find in Indonesia. This species of algae known to have a lot of potential, which until now unknown. One potential that tried to explore in this research is the ability of these algae for decreasing the level of salt water salinity into fresh water. This research is an experiment with a completely randomized design (CRD), three treatments and three replications. Testing is done by performing algae arbsorbtion using three forms (powder, granule, and paper) with different biomass (1 g, 2 g, and 4gr). Preparations then coupled into a transparent pipe. The results showed the real effect of the decreased levels of salinity brine through the circuit. Filter with the powder forms with 4gr biomass has the highest salinity level reduction of 15% o, then the dosage form of granules with biomass 4gr with decreased 5.23% o and the third dosage form of paper with 1 gram of biomass with a decrease of 4.23% o. Forms with other biomass have a decrease in the salinity level of not more than 4% o. Preparations with powder form and 4gr biomass can be used to reduce the salinity level in the salt water.
透明Spyrogyra hyalina是一种在印度尼西亚很容易找到的淡水藻类。这种藻类被认为具有很大的潜力,直到现在还不为人所知。在这项研究中试图探索的一个潜力是这些藻类降低淡水含盐量的能力。本研究采用完全随机设计(CRD),三个处理,三个重复。测试是通过使用三种形式(粉状、颗粒状和纸状)的不同生物量(1克、2克和4克)进行藻类吸收来完成的。然后将准备耦合到透明管道中。实验结果表明,通过回路降低盐水含盐量的实际效果。过滤器与4 gr生物质粉末形式盐度最高水平减少15%啊,然后用生物质颗粒剂型的4 gr与降低5.23% o和论文的第三个剂型与1克生物量减少4.23%的o。形式与其他生物的盐度水平下降不超过4%粉状和o。准备4 gr生物质可以用来减少海水的盐度水平。
{"title":"Biofiltration Efficiency of Algae Spirogyra hyalina to Reduce Salinity of Sea Water","authors":"A. Putra","doi":"10.20956/IJAB.V1I1.2003","DOIUrl":"https://doi.org/10.20956/IJAB.V1I1.2003","url":null,"abstract":"Algae Spyrogyra hyalina is one species of fresh water algae that is very easy to find in Indonesia. This species of algae known to have a lot of potential, which until now unknown. One potential that tried to explore in this research is the ability of these algae for decreasing the level of salt water salinity into fresh water. This research is an experiment with a completely randomized design (CRD), three treatments and three replications. Testing is done by performing algae arbsorbtion using three forms (powder, granule, and paper) with different biomass (1 g, 2 g, and 4gr). Preparations then coupled into a transparent pipe. The results showed the real effect of the decreased levels of salinity brine through the circuit. Filter with the powder forms with 4gr biomass has the highest salinity level reduction of 15% o, then the dosage form of granules with biomass 4gr with decreased 5.23% o and the third dosage form of paper with 1 gram of biomass with a decrease of 4.23% o. Forms with other biomass have a decrease in the salinity level of not more than 4% o. Preparations with powder form and 4gr biomass can be used to reduce the salinity level in the salt water.","PeriodicalId":14981,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"66 1","pages":"9-13"},"PeriodicalIF":0.0,"publicationDate":"2017-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82192148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Journal of Applied Biology & Biotechnology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1