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The Juvenoids Methoprene and Pyriproxyfen Affect Female Fecundity in Anastrepha ludens but Not in Ceratitis capitata Vienna-8 甲氧戊二烯和吡丙醚对绿斑大鼠雌性繁殖力有影响,但对维也纳头角蛇尾无影响
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-19 DOI: 10.1111/jen.70020
Florida López-Arriaga, María de Lourdes Zaragoza-Martínez, José Arredondo, María Guadalupe Rodríguez-Galván, Francisco Díaz-Fleischer

The sterile insect technique (SIT) is considered a tool for sustainable pest control and is known to substantially improve the control of economically important fruit flies through male-only releases facilitated by the mass-rearing of genetic sexing strains. However, the production of these strains is more costly because adult colonies must be at least three times larger than those of bisexual strains in order to produce the same number of eggs. The present study determined the viability of increasing the egg production of the genetic sexing strain Vienna 8 of Ceratitis capitata (Wiedemann) and the bisexual strain of Anastrepha ludens (Loew) by using the juvenoids methoprene and pyriproxyfen to stimulate female fecundity. We evaluated doses of 0.05%, 0.03% and 0.01%, which were incorporated into the adult diet. We determined the effect on potential female fecundity, female fecundity, female fertility, and male and female survival in both species. The results showed that the methoprene and pyriproxyfen doses used did not have an effect on potential fecundity, fecundity and fertility in females of C. capitata, but there was an effect in females of A. ludens. The juvenoids also had a negative effect on adult survival in both species. In conclusion, using either of the two juvenoids can increase egg production in a mass-rearing facility of A. ludens but not of C. capitata, and their application requires further evaluation.

昆虫不育技术(SIT)被认为是一种可持续害虫控制的工具,已知通过大量饲养具有遗传性别的菌株促进雄性只释放,从而大大改善了对经济上重要的果蝇的控制。然而,这些菌株的生产成本更高,因为为了产生相同数量的卵,成年菌落必须至少比双性菌株大三倍。本研究确定了用甲氧丙烯和吡丙氧芬刺激雌性繁殖力对头角Ceratitis capitata (Wiedemann)遗传性别菌株Vienna 8和Anastrepha ludens (Loew)两性菌株增加产卵量的可行性。我们评估了0.05%、0.03%和0.01%的剂量,这些剂量被纳入成人饮食。我们测定了对两个物种的潜在雌性繁殖力、雌性繁殖力、雌性繁殖力和雌雄存活率的影响。结果表明,甲氧丁烯和吡丙醚剂量对大花田鼠雌虫的潜在繁殖力、繁殖力和育力均无影响,但对大花田鼠雌虫有影响。幼体对两种成虫的存活率也有负面影响。综上所述,在大规模饲养设施中,使用两种幼虫均可提高黄颡鱼的产蛋量,但对头形黄颡鱼没有效果,两者的应用有待进一步评价。
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引用次数: 0
Is Nocturnal Pollination Important for Crop Production? Experimental Evidence From Small Fruit Crops 夜间授粉对作物生产很重要吗?小型水果作物的实验证据
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-12 DOI: 10.1111/jen.70019
Elsa Blareau, Fabrice Requier

Insect-mediated pollination is essential for crop production but is mainly studied considering diurnal pollinators only. Here, we use pollinator exclusion techniques to prevent either diurnal or nocturnal insect visits in small fruit crops: raspberry (Rubus idaeus), over 1 year, and red currant (Ribes rubrum) and black currant (Ribes nigrum), over 2 years, in sites within mixed landscapes of western and northern France. The contribution of day- and night-active pollinators was measured from 850 flowers total from 36 plants across all species and included the following pollination components: fruit set, fruit weight, fruit volume and seed set. We find that nocturnal pollination improves seed set of all three crops, as well as fruit volume of red currant, compared to exclusion of all insect visits. This confirms the existence of nocturnal pollination services for these crops. We found no direct evidence of a nocturnal pollination service for fruit set of the three crops. However, we found that contributions from the combination of diurnal and nocturnal pollinators enhance fruit set in red currant and black currant. We found no effects of pollination treatment on fruit weight of any crop. These results suggest that nocturnal pollination plays a role in the production of small fruit crops, and call for inclusive pollinator-friendly schemes including night-active pollinators in order to support pollination services and crop production.

昆虫传粉对作物生产至关重要,但主要研究只考虑昼夜传粉者。在这里,我们使用授粉者排除技术来防止小型水果作物的白天或夜间昆虫来访:覆盆子(Rubus idaeus),超过1年,红醋栗(Ribes rubrum)和黑醋栗(Ribes nigrum),超过2年,在法国西部和北部混合景观的地点。研究人员从36种植物的850朵花中测量了白天和夜间活动传粉者的贡献,包括以下授粉成分:坐果量、果实重量、果实体积和坐果量。我们发现,与排除所有昆虫来访相比,夜间授粉改善了这三种作物的结实率,以及红醋栗的果实体积。这证实了这些作物夜间授粉服务的存在。我们没有发现这三种作物果实的夜间授粉服务的直接证据。然而,我们发现昼夜传粉者和夜间传粉者对红醋栗和黑醋栗的坐果有促进作用。我们没有发现授粉处理对任何作物的果实重量有影响。这些结果表明,夜间授粉在小型水果作物的生产中起着重要作用,并呼吁制定包括夜间活跃传粉者在内的包容性传粉者友好计划,以支持授粉服务和作物生产。
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引用次数: 0
Effects of Different Combinations of Sterile and Fertile Thaumatotibia leucotreta on Fruit Infestation and Population Growth Rate 不同不育与可育组合对白桦果实侵染及种群生长率的影响
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-12 DOI: 10.1111/jen.70018
Michael M. Githae, Candice A. Coombes, Reyard Mutamiswa, Sean D. Moore, Martin P. Hill

Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is a key phytosanitary pest of citrus in South Africa. The pest damages fruit by burrowing and feeding on the pulp, thus causing yield losses and export rejections. There are several T. leucotreta management strategies, such as orchard sanitation, mating disruption, attract-and-kill, the use of biocontrol agents, and the sterile insect technique. A laboratory cage study was conducted to examine the impact of different combinations of treated (sterile) and untreated (fertile) T. leucotreta adults on fruit damage and reproduction rate. Thaumatotibia leucotreta adults were grouped into different combinations of treated (T) and untreated (U) male (M) and female (F): UM × UF (control), TM × UF, UM × TF, TM × TF, and UM × UF × TM × TF. These combinations were released into cages to mate and oviposit on the Navel oranges. Each treatment was replicated three times, and the experiment was repeated three times. After 4 weeks, the number of damaged fruit and larval entries per fruit per cage were recorded. Damaged fruit per treatment were then incubated until all emerging F1 progeny were collected and outcrossed with fertile T. leucotreta. Fecundity and fertility per treatment were recorded. Cages with sterile T. leucotreta had significantly fewer damaged fruit, larval entries, and emerged F1 adults compared to the control, except for UM × UF × TM × TF treatments. Similarly, control cages and UM × UF × TM × TF treatments had significantly higher fecundity and fertility compared to other treatments involving sterile T. leucotreta. The TM × UF combination exhibited the lowest rate of increase per generation (< 0.57× from the parental to F1 generation), demonstrating a reduction in the fertile population. The results demonstrated that the release of sterile T. leucotreta leads to sterile-fertile matings rather than fertile-fertile matings, thereby aiding in pest suppression in the T. leucotreta SIT programme.

摘要白斑蝽(thoumatotibia leucotreta (Meyrick))是南非柑橘的主要植物检疫害虫。这种害虫通过挖洞和啃食果肉来破坏水果,从而造成产量损失和出口拒收。白曲甲的防治策略有果园卫生、干扰交配、诱杀、生物防治剂的使用和昆虫不育技术等。通过室内笼试验,研究了不同处理(不育)和未处理(可育)白骨锥虫成虫组合对果实损伤和繁殖率的影响。将成年白鲟按治疗组(T)和未治疗组(U)分为雄性(M)和雌性(F),分别为UM × UF(对照)、TM × UF、UM × TF、TM × TF和UM × UF × TM × TF。这些组合被释放到笼子里,在脐橙上交配和产卵。每个处理重复3次,实验重复3次。4周后,记录每笼被害果数和每个果幼虫数。然后将每个处理的受损果实进行孵育,直到收集所有出苗的F1后代,并与可育的白骨锥虫进行异交。记录每次处理的繁殖力和育力。除UM × UF × TM × TF处理外,无菌处理的白鲟损伤果实、幼虫幼虫数和出苗数均显著低于对照。同样,对照笼和UM × UF × TM × TF处理的繁殖能力和育性也显著高于其他处理。TM × UF组合每代增加率最低(从亲本到F1代增加<; 0.57×),表明可育群体减少。结果表明,释放不育的白鲟导致不育的交配而不是可育的交配,从而有助于白鲟SIT项目中害虫的抑制。
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引用次数: 0
Development of China's Crop Disease and Pest Monitoring and Forecasting System: A Comprehensive Review 中国作物病虫害监测预报系统的发展综述
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-10 DOI: 10.1111/jen.70017
Shuxiong Bai, Yong Zhang, Zhanfeng Yan, Jingfei Guo, Yueqin Wang, Bo Zhang, Xianming Yang, Feng Zhang, Zhenying Wang

Effective management of crop diseases and pests is essential for ensuring global food security and promoting sustainable agriculture. Over the past decades, China has developed a sophisticated Crop Disease and Pest Monitoring and Forecasting (CDPMF) system, which has significantly enhanced agricultural productivity. This study presents a critical synthesis of China's CDPMF system's evolution, critically analysing its milestones and experiences in organisational structure, technological innovation, information technology implementation, talent cultivation and international cooperation. The findings highlight that China has made substantial progress in pest and disease monitoring and early warning through the establishment of a five-tier nationwide monitoring network, the introduction of advanced technologies and the promotion of international collaboration. These achievements provide critical references for global sustainable agricultural development.

有效管理作物病虫害对确保全球粮食安全和促进可持续农业至关重要。在过去的几十年里,中国建立了先进的作物病虫害监测与预报系统,显著提高了农业生产力。本研究对中国CDPMF系统的演变进行了批判性的综合,批判性地分析了其在组织结构、技术创新、信息技术实施、人才培养和国际合作方面的里程碑和经验。研究结果强调,通过建立五层全国监测网络、引进先进技术和促进国际合作,中国在病虫害监测和预警方面取得了实质性进展。这些成果为全球农业可持续发展提供了重要参考。
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引用次数: 0
Biosurveillance for an Invasive Pest of Maize, Prostephanus truncatus, Across North America and in Greece 北美及希腊玉米入侵害虫扁扁螟的生物监测
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-08 DOI: 10.1111/jen.70016
Hannah E. Quellhorst, Maria K. Sakka, Sylvanus Odjo, Dalton Ludwick, Georgina V. Bingham, Karl A. Roeder, Louis S. Hesler, Raina M. Hanley, Junwei J. Zhu, Rachel V. Wilkins, Marco A. Ponce, Christos G. Athanassiou, Kun Yan Zhu, Alison R. Gerken, William R. Morrison III

Biosecurity is an increasingly important aspect of management of commodities in a globalised economy. The larger grain borer, Prostephanus truncatus, is a known biosecurity threat to the United States and has been classified as a species-of-concern. Under climate change, P. truncatus is expected to expand its range into the United States from Mexico. In this study, we performed a 2-year latitudinal biosurveillance programme for P. truncatus, related bostrichids and Sitophilus spp. as key species in and around maize and wheat production at 10–15 sites from 20° to 47° latitude in North America and Greece to evaluate how habitat, latitude and season affected spatiotemporal dynamics of these insects. Two types of traps, a 4-funnel Lindgren and pitfall traps, were deployed at each site, baited with each species' pheromones. In total, we captured 2528 insects in 2021 and 29,080 insects in 2022. P. truncatus was only documented in Mexico but in high abundance in both years. Interestingly, even though P. truncatus is a good flier, the funnel trap was equally effective as the pitfall trap in monitoring P. truncatus. Conspecifics were found most often in natural and row crop habitats in both years compared to food facilities. Captures of P. punctatus in 2022 most often occurred in natural habitats, but they were still present at row crops and near food facilities. By contrast, Sitophilus spp. were most associated with food facilities. Overall, this study helps inform current risk from P. truncatus and fundamental patterns of spatiotemporal distribution for several related key stored product insects.

在全球化经济中,生物安全是商品管理日益重要的方面。更大的谷物螟虫(Prostephanus truncatus)是美国已知的生物安全威胁,已被列为值得关注的物种。在气候变化的影响下,圆木将从墨西哥扩展到美国。本研究在北美和希腊20°~ 47°纬度的10 ~ 15个地点,对玉米和小麦生产及其周围的主要昆虫——圆腹圆蚧及其相关的乳蚧和象蛉进行了为期2年的纬度生物监测,以评估生境、纬度和季节对这些昆虫时空动态的影响。在每个地点部署了两种类型的陷阱,即4漏斗林格伦陷阱和陷阱陷阱,并以每种物种的信息素为诱饵。我们在2021年捕获了2528只昆虫,在2022年捕获了29,080只昆虫。在这两个年份中,只在墨西哥有记录,但丰度都很高。有趣的是,尽管白杨是一种优秀的飞行者,但漏斗诱捕器与陷阱诱捕器在监测白杨方面同样有效。与食品设施相比,这两年在自然和行作物生境中发现同种虫最多。2022年斑马鱼的捕获最常发生在自然栖息地,但它们仍然存在于行作物和食品设施附近。与此相反,象蛉与食物设施的关系最为密切。总体而言,该研究有助于了解白杨的风险现状和几种相关关键储藏产品昆虫的基本时空分布格局。
{"title":"Biosurveillance for an Invasive Pest of Maize, Prostephanus truncatus, Across North America and in Greece","authors":"Hannah E. Quellhorst,&nbsp;Maria K. Sakka,&nbsp;Sylvanus Odjo,&nbsp;Dalton Ludwick,&nbsp;Georgina V. Bingham,&nbsp;Karl A. Roeder,&nbsp;Louis S. Hesler,&nbsp;Raina M. Hanley,&nbsp;Junwei J. Zhu,&nbsp;Rachel V. Wilkins,&nbsp;Marco A. Ponce,&nbsp;Christos G. Athanassiou,&nbsp;Kun Yan Zhu,&nbsp;Alison R. Gerken,&nbsp;William R. Morrison III","doi":"10.1111/jen.70016","DOIUrl":"https://doi.org/10.1111/jen.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>Biosecurity is an increasingly important aspect of management of commodities in a globalised economy. The larger grain borer, <i>Prostephanus truncatus</i>, is a known biosecurity threat to the United States and has been classified as a species-of-concern. Under climate change, <i>P. truncatus</i> is expected to expand its range into the United States from Mexico. In this study, we performed a 2-year latitudinal biosurveillance programme for <i>P. truncatus</i>, related bostrichids and <i>Sitophilus</i> spp. as key species in and around maize and wheat production at 10–15 sites from 20° to 47° latitude in North America and Greece to evaluate how habitat, latitude and season affected spatiotemporal dynamics of these insects. Two types of traps, a 4-funnel Lindgren and pitfall traps, were deployed at each site, baited with each species' pheromones. In total, we captured 2528 insects in 2021 and 29,080 insects in 2022. <i>P. truncatus</i> was only documented in Mexico but in high abundance in both years. Interestingly, even though <i>P. truncatus</i> is a good flier, the funnel trap was equally effective as the pitfall trap in monitoring <i>P. truncatus</i>. Conspecifics were found most often in natural and row crop habitats in both years compared to food facilities. Captures of <i>P. punctatus</i> in 2022 most often occurred in natural habitats, but they were still present at row crops and near food facilities. By contrast, <i>Sitophilus</i> spp. were most associated with food facilities. Overall, this study helps inform current risk from <i>P. truncatus</i> and fundamental patterns of spatiotemporal distribution for several related key stored product insects.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"150 1","pages":"115-127"},"PeriodicalIF":2.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145930990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-Invasive Computer Vision-Based Fruit Fly Larvae Differentiation: Ceratitis capitata and Bactrocera zonata 基于非侵入性计算机视觉的果蝇幼虫分化:头角蝇和带状小实蝇
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-08 DOI: 10.1111/jen.70009
Eddie Kanevsky, Teddy Lazebnik, Roy Kaspi, Yoav Gazit, Eyal Halon, Dror Fried, Anna Zamansky, Gur Pines

The Mediterranean fruit fly (Ceratitis capitata) and the peach fruit fly (Bactrocera zonata) are two of the most economically significant agricultural pests affecting fruit production worldwide. Both are considered quarantine pests in several countries, which oblige the use of restrictive measures to assure safe trade with countries where these flies are present. As the quarantine status of these two pests is not similar in every country, discriminating measures among these two fruit flies' larvae in the exported fruits is critical for safe trade. Traditional DNA-based detection methods, though accurate, are costly and time-consuming, while manual morphological identification is practically impossible. In this study, we propose a novel non-invasive method utilising computer vision for rapid differentiation between larvae of these two species based on a short video recording of a single larva freely moving on a Petri dish. Our results reveal good separation between the two species with 90% accuracy using videos as short as 15 s long.

地中海果蝇(Ceratitis capitata)和桃果蝇(Bactrocera zonata)是影响全球水果生产的两种最具经济意义的农业害虫。在一些国家,这两种蝇都被视为检疫性有害生物,因此必须采取限制性措施,以确保与存在这些蝇类的国家进行安全贸易。由于各国对这两种害虫的检疫情况不尽相同,因此对出口水果中这两种果蝇幼虫的鉴别措施对安全贸易至关重要。传统的基于dna的检测方法虽然准确,但成本高,耗时长,而人工形态识别实际上是不可能的。在这项研究中,我们提出了一种新的非侵入性方法,利用计算机视觉快速区分这两个物种的幼虫,该方法基于单个幼虫在培养皿上自由移动的短视频记录。我们的研究结果表明,使用短至15秒长的视频可以很好地区分两个物种,准确率达到90%。
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引用次数: 0
Correction to “First report of Chilo partellus in Turkey, a new invasive maize pest for Europe” 更正“欧洲新入侵玉米害虫——土尔其的首次报告”
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-03 DOI: 10.1111/jen.70015

Bayram, A. and Tonğa, A. (2016), First report of Chilo partellus in Turkey, a new invasive maize pest for Europe. J. Appl. Entomol., 140: 236-240. https://doi.org/10.1111/jen.12232.

We wish to clarify that Sertkaya et al. (2014) previously reported Chilo partellus in Turkey in the Türkiye Entomoloji Bülteni (https://dergipark.org.tr/en/pub/entoteb/article/75077), a regional journal not indexed in any major international scientific databases. As this publication was not discoverable through standard literature searches (e.g., Web of Science, CAB Abstracts), it was unknown to us during the preparation of our manuscript. Our study includes authenticated voucher specimens, independent expert confirmation and additional ecological data, thereby complementing and expanding upon the earlier report.

We apologize for this error.

Bayram, a .和Tonğa, a .(2016),欧洲新型玉米入侵害虫——partellus在土耳其的首次报道。j:。Entomol。中文信息学报,14(4):344 - 344。https://doi.org/10.1111/jen.12232.We希望澄清,Sertkaya et al.(2014)之前在《 rkiye Entomoloji b lteni》(https://dergipark.org.tr/en/pub/entoteb/article/75077)上报道了土耳其的Chilo partellus,该区域性期刊未被任何主要国际科学数据库检索。由于该出版物无法通过标准文献搜索(例如,Web of Science, CAB Abstracts)发现,因此在准备手稿时我们对其一无所知。我们的研究包括经过认证的凭证标本,独立专家确认和额外的生态数据,从而补充和扩展了先前的报告。我们为这个错误道歉。
{"title":"Correction to “First report of Chilo partellus in Turkey, a new invasive maize pest for Europe”","authors":"","doi":"10.1111/jen.70015","DOIUrl":"https://doi.org/10.1111/jen.70015","url":null,"abstract":"<p>Bayram, A. and Tonğa, A. (2016), First report of <i>Chilo partellus</i> in Turkey, a new invasive maize pest for Europe. J. Appl. Entomol., 140: 236-240. https://doi.org/10.1111/jen.12232.</p><p>We wish to clarify that Sertkaya et al. (2014) previously reported <i>Chilo partellus</i> in Turkey in the <i>Türkiye Entomoloji Bülteni</i> (https://dergipark.org.tr/en/pub/entoteb/article/75077), a regional journal not indexed in any major international scientific databases. As this publication was not discoverable through standard literature searches (e.g., Web of Science, CAB Abstracts), it was unknown to us during the preparation of our manuscript. Our study includes authenticated voucher specimens, independent expert confirmation and additional ecological data, thereby complementing and expanding upon the earlier report.</p><p>We apologize for this error.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145426112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a Modular 3D-Printed Pollen Trap for Bumble Bee Monitoring 用于大黄蜂监测的模块化3d打印花粉捕获器的开发
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-09-01 DOI: 10.1111/jen.70011
Richard Odemer, Marie Geiger, Lars Geiger

Accurate pollen collection is essential for understanding bumble bee foraging dynamics, assessing environmental risks and monitoring colony health. Effective monitoring systems provide critical insights into pesticide exposure, floral resource availability and pollinator health. This study compares the efficiency of two pollen trap designs, the newly developed JKI trap and the USDA 3D-printed trap, in collecting pollen from Bombus terrestris colonies. Field tests using traps with two entrance diameters (6.5 and 7.2 mm) showed that the JKI trap collected significantly more pollen than the USDA trap, with the statistical model predicting approximately 24 times higher yields (p < 0.001); no significant effect of entrance diameter on pollen yield was observed. The JKI trap's effective performance, coupled with its design flexibility and potential for adaptation across different Bombus species and pollinators, makes it a valuable tool for long-term ecological monitoring, floral resource assessments, and pesticide risk studies.

准确的花粉采集对于了解大黄蜂觅食动态、评估环境风险和监测群体健康至关重要。有效的监测系统提供了农药暴露、植物资源可用性和传粉者健康的关键信息。本研究比较了两种花粉诱捕器设计的效率,即新开发的JKI诱捕器和美国农业部3d打印的诱捕器,在收集地蜂菌落花粉方面的效率。采用两种入口直径(6.5和7.2 mm)的诱捕器进行的田间试验表明,JKI诱捕器收集的花粉量显著高于USDA诱捕器,统计模型预测的产量约为USDA诱捕器的24倍(p < 0.001);入口直径对花粉产量无显著影响。JKI诱捕器的有效性能,加上其设计的灵活性和适应不同种类和传粉媒介的潜力,使其成为长期生态监测、花卉资源评估和农药风险研究的宝贵工具。
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引用次数: 0
Development of a LAMP-Based Molecular Species Diagnostic Tool for the Invasive Pest, Tuta absoluta and Phthorimaea operculella (Lepidoptera, Gelechiidae) 基于lamp的入侵害虫绝对灰蛾和细鳞灰蛾分子物种诊断工具的研制(鳞翅目,蛭科)
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-08-30 DOI: 10.1111/jen.70010
Hoki Hong, Murtaza Khan, Minyoung Choi, Minseop Noh, Sejeong Hwang, Youngsu Lee, Alicia Timm, Juil Kim

The tomato leaf miner, Tuta absoluta, is a destructive and rapidly spreading invasive pest that poses a major threat to tomato production worldwide. Native to South America, this species has expanded into Africa, Europe, Asia, and, more recently, has been detected in Korea. Management of T. absoluta is particularly challenging due to its high reproductive potential, resistance to commonly used insecticides, and morphological similarity to sympatric gelechiid pests such as Phthorimaea operculella, which complicate accurate identification. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid and specific detection of T. absoluta, utilising diagnostic markers from the mitochondrial genome. The assay was optimised to function effectively at 61°C and employs a set of species-specific primers that enable amplification within 20 min. It demonstrated high sensitivity, reliably detecting as little as 100 femtograms of genomic DNA. To support field applicability, we established a rapid and simplified tissue-based DNA extraction method that requires minimal laboratory infrastructure. Specificity and reliability were validated using samples collected from multiple geographic locations, ensuring robustness across populations. The combination of speed, sensitivity, and ease of use makes this assay highly suitable for field surveillance, quarantine inspection, and early detection efforts. This molecular diagnostic tool offers a cost-effective and practical approach for the identification of T. absoluta, contributing significantly to integrated pest management programmes and the mitigation of economic losses in tomato-producing regions worldwide.

番茄叶螨(Tuta absoluta)是一种破坏性和迅速蔓延的入侵害虫,对全球番茄生产构成重大威胁。原产于南美洲,该物种已扩展到非洲,欧洲,亚洲,最近在韩国被发现。由于其较高的繁殖潜力,对常用杀虫剂的抗性,以及与同域害虫(如薄皮蝇)的形态相似性,使其难以准确识别,因此对绝对白蝇的管理尤其具有挑战性。在这项研究中,我们开发了一种环介导的等温扩增(LAMP)方法,利用线粒体基因组的诊断标记快速特异性检测T. absoluta。该实验经过优化,可在61°C下有效工作,并采用一组物种特异性引物,可在20分钟内扩增。它具有很高的灵敏度,可以可靠地检测到100飞图的基因组DNA。为了支持现场适用性,我们建立了一种快速和简化的基于组织的DNA提取方法,需要最少的实验室基础设施。从多个地理位置收集的样本验证了特异性和可靠性,确保了人群的稳健性。速度,灵敏度和易用性的结合使这种分析非常适合现场监测,检疫检查和早期检测工作。这种分子诊断工具提供了一种具有成本效益和实用的方法来鉴定绝对番茄,为全球番茄产区的病虫害综合治理规划和减轻经济损失作出重大贡献。
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引用次数: 0
Pre-Bloom Application of Petroleum Spray Oil for the Control of Green Apple Aphid, Aphis pomi De Geer in Apple Orchards 花前喷油防治苹果园青苹果蚜虫的研究
IF 2 3区 农林科学 Q2 ENTOMOLOGY Pub Date : 2025-08-29 DOI: 10.1111/jen.70013
Mohd Abas Shah, Hafsa Ajaz Tramboo, Sheikh Aafreen Rehman, Rifat Rasool, Kawsar Rasool, Shahid Yaqub, Ronit Jaiswal, Sajad Un Nabi

The green apple aphid (Aphis pomi De Geer) is a major early-season pest in apple orchards. While petroleum spray oils (PSOs) are commonly applied at the delayed dormant stage to control San José scale and European red mite, their effect on A. pomi remains unclear. This study assessed the ovicidal and larvicidal efficacy of 2% PSO applications at the delayed dormant/green tip stage. Laboratory and field assays demonstrated substantial suppression of egg hatch, with cumulative hatch reduced to 9.90% ± 0.57% (laboratory assay) and 9.62% ± 0.71% (field evaluation) in treated shoots. Survival analyses revealed significantly higher mortality risk for treated eggs. Direct toxicity trials on nymphs showed mortality rates of 69.9% ± 1.95% in field conditions and > 99% in lab dip assays by 5 days after treatment. Residual toxicity assays indicated cumulative mortality reaching 64.07% ± 2.74% by 2 days post-exposure, confirming efficacy even after drying. Field trials further revealed that PSO significantly reduced bud infestation by A. pomi (5.22% ± 0.54%) compared to the untreated control (19.04% ± 2.34%), with enhanced suppression observed when combined with imidacloprid (0.29% ± 0.14%). These findings underscore the potential of PSO as both a standalone and synergistic agent in aphid management. This is the first detailed report on the ovicidal and larvicidal activity of PSOs against A. pomi, supporting its inclusion in sustainable, early-season integrated pest management programmes.

青苹果蚜虫是苹果园早期的主要害虫。虽然石油喷雾油(pso)通常在延迟休眠阶段用于控制圣何塞鳞螨和欧洲红螨,但其对pomi的效果尚不清楚。本研究评估了2% PSO在延迟休眠/绿尖期的杀卵和杀幼虫效果。室内和现场试验均表明,处理后的幼苗的累计孵化率分别降至9.90%±0.57%(实验室测定)和9.62%±0.71%(现场评价)。生存分析显示,处理过的卵子死亡风险明显更高。对若虫直接毒性试验表明,处理后5 d,田间死亡率为69.9%±1.95%,实验室浸液试验死亡率为>; 99%。残留毒性试验表明,暴露后2天的累积死亡率为64.07%±2.74%,即使干燥后也有效。田间试验进一步表明,与未处理对照(19.04%±2.34%)相比,PSO显著降低了pomi的芽侵染率(5.22%±0.54%),与吡虫啉联用抑制效果更强(0.29%±0.14%)。这些发现强调了PSO在蚜虫管理中作为单独和协同剂的潜力。这是第一份关于pso对pomi的杀卵和杀幼虫活性的详细报告,支持将其纳入可持续的早期病虫害综合治理规划。
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Journal of Applied Entomology
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