Pub Date : 2021-01-01DOI: 10.21608/aprh.2021.77133.1132
Basma M. Tawfik, M. Rizk, M. Sultan, R. El-Eryan
Objectives: In this work we presented a highly sensitive, selective and validated method for determination of tedizolid phosphate (TEDP) antibiotic, based on its native fluorescence in aqueous solution. Methods: The maximum fluorescence intensity was measured at 408 nm after excitation at 298 nm after optimization of all experimental conditions. Results: The measured fluorescence was directly proportional to the concentration of the drug over the range of 2-30 ng/mL with a limit of detection of 0.13 and limit of quantification of 0.44 ng/mL The method succeeded to determine TEDP in its pharmaceutical dosage form and in human plasma with mean % recovery of 100.49 ±1.32 and 99.40 ± 2.18 respectively. Conclusion: The developed data was found to be with a good agreement with a valid method. The method was validated according to ICH guidelines for determination of the drug in its pure form and dosage form and according to FDA Guidance for Industry, Bioanalytical Method Validation for determination of TEDP in human plasma.
{"title":"Highly Sensitive, Selective and Validated Spectrofluorimetric Assay for Novel Oxazolidinone Antibiotic: Tedizolid Phosphate in Pharmaceutical Dosage Form and Human Plasma","authors":"Basma M. Tawfik, M. Rizk, M. Sultan, R. El-Eryan","doi":"10.21608/aprh.2021.77133.1132","DOIUrl":"https://doi.org/10.21608/aprh.2021.77133.1132","url":null,"abstract":"Objectives: In this work we presented a highly sensitive, selective and validated method for determination of tedizolid phosphate (TEDP) antibiotic, based on its native fluorescence in aqueous solution. Methods: The maximum fluorescence intensity was measured at 408 nm after excitation at 298 nm after optimization of all experimental conditions. Results: The measured fluorescence was directly proportional to the concentration of the drug over the range of 2-30 ng/mL with a limit of detection of 0.13 and limit of quantification of 0.44 ng/mL The method succeeded to determine TEDP in its pharmaceutical dosage form and in human plasma with mean % recovery of 100.49 ±1.32 and 99.40 ± 2.18 respectively. Conclusion: The developed data was found to be with a good agreement with a valid method. The method was validated according to ICH guidelines for determination of the drug in its pure form and dosage form and according to FDA Guidance for Industry, Bioanalytical Method Validation for determination of TEDP in human plasma.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89706703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-28DOI: 10.21608/aprh.2020.42333.1115
Ahmed G. Abd Elhameed, Sara N. Suliman, Marwa Elsbaey, Mai M. Elnaggar, F. Badria
Objective: The current study aimed to investigate and compare the efficiency of the total extract and ethyl acetate (EtOAc) extract of Mangifera indica leaves to attenuate iron overload-induced hepatic and splenic dysfunctions, using an in-vivo iron-overload rat model. Methods: Rats received water supplemented with iron for 2 months, followed by treatment with either total extract or EtOAc extract of M. indica leaves for an extra 2 months. Results: Rats treated with EtOAc fraction of M. indica leaves, and the total extract, had significantly diminished iron accumulation within hepatic and splenic tissues. Both extracts normalized the hepatic enzymatic and non-enzymatic biomarkers like alkaline phosphatase (ALP), alanine aminotransferase (AST), and total bilirubin. Moreover, they restored the oxidants/antioxidants balance within the liver and spleen tissues. Additionally, they suppressed HO-1 levels, increased Nrf2 expression, and downregulated MMP-9 expression. Conclusion: Briefly, M. indica extracts showed iron-chelation, antioxidant, and antifibrotic potentials. These findings pointed out the usefulness of the mango leaves as a natural, affordable by-product in managing iron overload complications. However, the results also demonstrated that EtOAc extract is more accountable for such activities.
{"title":"Mangifera indica Leaves Extracts Mitigate Experimentally Induced Oxidative Stress and Iron-Overload via Iron Chelation and Modulation of HO-1, Nrf2, and MMP-9","authors":"Ahmed G. Abd Elhameed, Sara N. Suliman, Marwa Elsbaey, Mai M. Elnaggar, F. Badria","doi":"10.21608/aprh.2020.42333.1115","DOIUrl":"https://doi.org/10.21608/aprh.2020.42333.1115","url":null,"abstract":"Objective: The current study aimed to investigate and compare the efficiency of the total extract and ethyl acetate (EtOAc) extract of Mangifera indica leaves to attenuate iron overload-induced hepatic and splenic dysfunctions, using an in-vivo iron-overload rat model. Methods: Rats received water supplemented with iron for 2 months, followed by treatment with either total extract or EtOAc extract of M. indica leaves for an extra 2 months. Results: Rats treated with EtOAc fraction of M. indica leaves, and the total extract, had significantly diminished iron accumulation within hepatic and splenic tissues. Both extracts normalized the hepatic enzymatic and non-enzymatic biomarkers like alkaline phosphatase (ALP), alanine aminotransferase (AST), and total bilirubin. Moreover, they restored the oxidants/antioxidants balance within the liver and spleen tissues. Additionally, they suppressed HO-1 levels, increased Nrf2 expression, and downregulated MMP-9 expression. Conclusion: Briefly, M. indica extracts showed iron-chelation, antioxidant, and antifibrotic potentials. These findings pointed out the usefulness of the mango leaves as a natural, affordable by-product in managing iron overload complications. However, the results also demonstrated that EtOAc extract is more accountable for such activities.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72850225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/aprh.2019.15686.1089
M. Ibrahim, A. Y. Mohamed, A. A. Ahmed
This paper describes an enhanced gas chromatography mass spectrometry (GC-MS) strategy for the analysis of Miconazole in water samples. In this study, determination of Miconazole has been carried out according to standard method for water and waste water analysis. Samples of collected water were agriculture stream water, River Nile water and Hospital waste water samples from El-Gharbia governorate in Egypt. Miconazole was extracted by solid - liquid extraction and analyzed by GC-MS. The chromatographic separation was performed using a ZB5 column (30 m ×0.53 mm, 1.50 µm), and helium as a carrier gas. The limit of detection and limit of quantification for Miconazole were 0.75and 2.50 ng/mL respectively. The intra- and inter-day precisions were lower than 0.85% while the accuracy ranged from 98.55% to 101.53. Finally, solid phase extraction (SPE) in combination with GC-MS is a sensitive and effective method for the determination of Miconazole in water samples.
本文介绍了水样中咪康唑的气相色谱-质谱分析方法。本研究按照水、废水分析标准方法进行了咪康唑的测定。收集的水样本是来自埃及加尔比亚省的农业溪流、尼罗河水和医院废水样本。采用固液萃取法提取咪康唑，GC-MS分析。采用ZB5色谱柱(30 m ×0.53 mm, 1.50µm)，氦气为载气进行色谱分离。咪康唑的检出限为0.75 ng/mL，定量限为2.50 ng/mL。日内、日间精密度均低于0.85%，准确度在98.55% ~ 101.53之间。最后，固相萃取-气相色谱-质谱联用是测定水样中咪康唑的一种灵敏、有效的方法。
{"title":"Development of a Method for the Determination of Miconazole in Water Samples Using Gas Chromatography Mass Spectrometry","authors":"M. Ibrahim, A. Y. Mohamed, A. A. Ahmed","doi":"10.21608/aprh.2019.15686.1089","DOIUrl":"https://doi.org/10.21608/aprh.2019.15686.1089","url":null,"abstract":"This paper describes an enhanced gas chromatography mass spectrometry (GC-MS) strategy for the analysis of Miconazole in water samples. In this study, determination of Miconazole has been carried out according to standard method for water and waste water analysis. Samples of collected water were agriculture stream water, River Nile water and Hospital waste water samples from El-Gharbia governorate in Egypt. Miconazole was extracted by solid - liquid extraction and analyzed by GC-MS. The chromatographic separation was performed using a ZB5 column (30 m ×0.53 mm, 1.50 µm), and helium as a carrier gas. The limit of detection and limit of quantification for Miconazole were 0.75and 2.50 ng/mL respectively. The intra- and inter-day precisions were lower than 0.85% while the accuracy ranged from 98.55% to 101.53. Finally, solid phase extraction (SPE) in combination with GC-MS is a sensitive and effective method for the determination of Miconazole in water samples.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"64 49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75403658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/aprh.2019.14237.1087
Abdel-hamid Ebid, S. Mohamed, A. Mira, A. Saleh
Objectives: This work was performed to study the pharmacokinetics of tacrolimus in adult liver transplant recipients after optimization of all the known classic factors contributing to inter-patient variability in whole blood tacrolimus levels. Also, to detect if any variability in whole blood tacrolimus levels still exists or this variability is only a function of the classic co-variables so that their optimization will diminish or eliminate it. Methods: Twenty-Six male patients with end-stage liver disease undergoing living donor liver transplantation were selected from the Gastroenterology Department of the International Medical Center, Cairo, Egypt, were enrolled in the study. A patient is initially considered to be a candidate for this study when tacrolimus was indicated as a part of a triple immune suppressive regimen with mycophenolate mofetil and prednisolone. Patients were selected to have non-significant variations in their demographics and pretreatment clinical data. Blood samples were drawn from each patient before the morning dose at specified intervals and the whole blood was assayed for tacrolimus, using Chemiluminescent Microparticle Immunoassay method (CMIA). Six months after liver transplantation, patients were classified into 3 groups based on their tacrolimus trough levels; normalized by its daily dose (C/D ratio), into fast, intermediate and slow metabolizers. Results: The results revealed unpredictable variability in whole blood tacrolimus levels among patients at each sampling time and a marked inter-patient variability in mean whole blood tacrolimus levels among individuals throughout the six months post transplantation period, (P value: <0.0001). Considerable inter-patient variability was also evident in tacrolimus pharmacokinetics. During 1st month post-transplant, tacrolimus C/D ratio varied from 0.53 to 12.2 (ng/ml*1/mg) and tacrolimus oral clearance (CL/F) varied from 3.4 to 79.4 L/hr. At 3rd month post-transplant, tacrolimus C/D ratio varied from 0.78 to 8.50 (ng/ml*1/mg) and tacrolimus CL/F varied from 4.9 to 53.2 L/hr. At 6th month post-transplant, tacrolimus C/D ratio varied from 0.73 to 7.10 (ng/ml*1/mg) and tacrolimus CL/F varied from 5.9 to 56.8 L/hr. The overall mean C/D ratio and oral clearance also showed a great variability among patients with a mean of 2.80±1.89 (CV: 67.5%) and 21.3±12.9 (CV:60.7%), respectively. Conclusion: The variability in whole blood tacrolimus concentrations and tacrolimus pharmacokinetics existed in spite of careful patient selection and optimization of all the classic co-variables known to affect tacrolimus concentrations, suggesting the presence of other unstudied factors; the recently evolving genetic factors might contribute to this variability. It is recommended to still considering therapeutic drug monitoring as an integral part of tacrolimus therapy to control variations in response until the discovery of a model that considers all the expected covariates to predict the respo
{"title":"Pharmacokinetics of Tacrolimus in Egyptian Liver Transplant Recipients: Role of the Classic Co-variables","authors":"Abdel-hamid Ebid, S. Mohamed, A. Mira, A. Saleh","doi":"10.21608/aprh.2019.14237.1087","DOIUrl":"https://doi.org/10.21608/aprh.2019.14237.1087","url":null,"abstract":"Objectives: This work was performed to study the pharmacokinetics of tacrolimus in adult liver transplant recipients after optimization of all the known classic factors contributing to inter-patient variability in whole blood tacrolimus levels. Also, to detect if any variability in whole blood tacrolimus levels still exists or this variability is only a function of the classic co-variables so that their optimization will diminish or eliminate it. Methods: Twenty-Six male patients with end-stage liver disease undergoing living donor liver transplantation were selected from the Gastroenterology Department of the International Medical Center, Cairo, Egypt, were enrolled in the study. A patient is initially considered to be a candidate for this study when tacrolimus was indicated as a part of a triple immune suppressive regimen with mycophenolate mofetil and prednisolone. Patients were selected to have non-significant variations in their demographics and pretreatment clinical data. Blood samples were drawn from each patient before the morning dose at specified intervals and the whole blood was assayed for tacrolimus, using Chemiluminescent Microparticle Immunoassay method (CMIA). Six months after liver transplantation, patients were classified into 3 groups based on their tacrolimus trough levels; normalized by its daily dose (C/D ratio), into fast, intermediate and slow metabolizers. Results: The results revealed unpredictable variability in whole blood tacrolimus levels among patients at each sampling time and a marked inter-patient variability in mean whole blood tacrolimus levels among individuals throughout the six months post transplantation period, (P value: <0.0001). Considerable inter-patient variability was also evident in tacrolimus pharmacokinetics. During 1st month post-transplant, tacrolimus C/D ratio varied from 0.53 to 12.2 (ng/ml*1/mg) and tacrolimus oral clearance (CL/F) varied from 3.4 to 79.4 L/hr. At 3rd month post-transplant, tacrolimus C/D ratio varied from 0.78 to 8.50 (ng/ml*1/mg) and tacrolimus CL/F varied from 4.9 to 53.2 L/hr. At 6th month post-transplant, tacrolimus C/D ratio varied from 0.73 to 7.10 (ng/ml*1/mg) and tacrolimus CL/F varied from 5.9 to 56.8 L/hr. The overall mean C/D ratio and oral clearance also showed a great variability among patients with a mean of 2.80±1.89 (CV: 67.5%) and 21.3±12.9 (CV:60.7%), respectively. Conclusion: The variability in whole blood tacrolimus concentrations and tacrolimus pharmacokinetics existed in spite of careful patient selection and optimization of all the classic co-variables known to affect tacrolimus concentrations, suggesting the presence of other unstudied factors; the recently evolving genetic factors might contribute to this variability. It is recommended to still considering therapeutic drug monitoring as an integral part of tacrolimus therapy to control variations in response until the discovery of a model that considers all the expected covariates to predict the respo","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78690080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/aprh.2019.17219.1090
M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag
antioxidant, antimicrobial and cytotoxic activities of the total methanolic extract and different fractions of Chrozophora oblongifolia root bark. Methods: Phytochemical screening of Chrozophora oblongifolia was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different 1D-NMR spectroscopy (1H NMR and 13C NMR). The antioxidant activity was evaluated using DPPH radical scavenging assay, antimicrobial activity was done by standard agar well diffusion assay and cytotoxic activity was performed by using MTT colorimetric assay method. Results: Phytochemical investigation of chrozophora oblongifolia revealed the presence ofcarbohydrates and / or glycosides, sterols and or triterpene, alkaloids, tannins and saponins and the absence of flavonoids and anthraquinones. After fractionation, methyl gallate (1), gallic acid (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from ethyl acetate fractions. The result of the antioxidant activity showed that, the ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and total methanolic extract. The result of the antimicrobial activity showed that, the total methanolic extract and ethyl acetate fraction showed moderate activity against Staphylococcus aureus, Vancomycin resistant Staphylococcus aureus and Candida albicans. The result of the cytotoxic activity showed that, the methylene chloride fraction followed by the total methanolic extract showed the highest cytotoxic activity against (MCF-7 and Huh-7) cancer cell lines. Conclusion: The root bark of Chrozophora oblongifolia is a rich source of different classes of active constituents as phenolics. The total extract and some fractions of Chrozophora oblongifolia showing antioxidant and antimicrobial and cytotoxic activities.
黄皮总甲醇提取物及不同部位抗氧化、抑菌和细胞毒活性的研究。方法:采用对每一类化合物进行特异性试验的方法，对黄参进行植物化学筛选。采用不同的色谱技术对化合物进行分离纯化，并利用不同的1D-NMR (1H NMR和13C NMR)对化合物结构进行了鉴定。用DPPH自由基清除法测定其抗氧化活性，用标准琼脂孔扩散法测定其抗菌活性，用MTT比色法测定其细胞毒活性。结果:经植物化学鉴定，黄花参中含有碳水化合物和/或糖苷、甾醇和/或三萜、生物碱、单宁和皂苷，不含黄酮类和蒽醌类化合物。从乙酸乙酯中分离得到没食子酸甲酯(1)、没食子酸(2)和β-谷甾醇-3- 0 -β- d -葡萄糖吡喃苷(3)。抗氧化活性结果表明，乙酸乙酯部位抗氧化活性最高，其次为正丁醇部位和总甲醇部位。抑菌活性结果表明，总甲醇提取物和乙酸乙酯部位对金黄色葡萄球菌、耐万古霉素金黄色葡萄球菌和白色念珠菌具有中等抑菌活性。细胞毒活性结果表明，二氯甲烷部位对MCF-7和Huh-7癌细胞的细胞毒活性最高，其次是总甲醇提取物。结论:桔梗根皮中含有丰富的酚类活性成分。黄花总提取物及部分部位具有抗氧化、抑菌和细胞毒活性。
{"title":"Phytochemical Investigation and Assessment of Antioxidant, Antimicrobial and Cytotoxic Activities of the Root Bark Chrozophora oblongifolia (Delile) Spreng. (Euphorbiaceae)","authors":"M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag","doi":"10.21608/aprh.2019.17219.1090","DOIUrl":"https://doi.org/10.21608/aprh.2019.17219.1090","url":null,"abstract":"antioxidant, antimicrobial and cytotoxic activities of the total methanolic extract and different fractions of Chrozophora oblongifolia root bark. Methods: Phytochemical screening of Chrozophora oblongifolia was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different 1D-NMR spectroscopy (1H NMR and 13C NMR). The antioxidant activity was evaluated using DPPH radical scavenging assay, antimicrobial activity was done by standard agar well diffusion assay and cytotoxic activity was performed by using MTT colorimetric assay method. Results: Phytochemical investigation of chrozophora oblongifolia revealed the presence ofcarbohydrates and / or glycosides, sterols and or triterpene, alkaloids, tannins and saponins and the absence of flavonoids and anthraquinones. After fractionation, methyl gallate (1), gallic acid (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from ethyl acetate fractions. The result of the antioxidant activity showed that, the ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and total methanolic extract. The result of the antimicrobial activity showed that, the total methanolic extract and ethyl acetate fraction showed moderate activity against Staphylococcus aureus, Vancomycin resistant Staphylococcus aureus and Candida albicans. The result of the cytotoxic activity showed that, the methylene chloride fraction followed by the total methanolic extract showed the highest cytotoxic activity against (MCF-7 and Huh-7) cancer cell lines. Conclusion: The root bark of Chrozophora oblongifolia is a rich source of different classes of active constituents as phenolics. The total extract and some fractions of Chrozophora oblongifolia showing antioxidant and antimicrobial and cytotoxic activities.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85148087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/aprh.2019.12303.1083
Khayrya A. Youssif, Ali M Elshamy, M. Rabeh, Nagwan M. Gabr, E. Haggag
Objectives: This study is aimed to be a comprehensive review of the phytochemical constituents and biological activities of Aizoaceae family plants (Mesembryanthemaceae). Methods: This study is covering articles between 1969 and 2018, reviewed from internationally accepted databases and scientific data from scientific Journals. Results: Phytochemically studied plants of family Aizoaceae have shown the presence of various classes of compounds including; alkaloids, triterpenes, sterols, lignans, phenolic compounds, betacyanins, and essential oils. Biological studies on plants of family Aizoaceae have indicated various bioactive potentials including antioxidant, antidiabetic, antimicrobial, antitumor, hepatoprotective, anti-inflammatory and other effects. The reported medicinal plants of family Aizoaceae were selected and summarized on the basis of their; phytochemical constituents and biological activities. Conclusion: The results of this study may inspire further ethno-botanical and ethno-pharmacological research and investigations toward drug discovery.
{"title":"A Phytochemical and Biological Review on Plants of The family Aizoaceae","authors":"Khayrya A. Youssif, Ali M Elshamy, M. Rabeh, Nagwan M. Gabr, E. Haggag","doi":"10.21608/aprh.2019.12303.1083","DOIUrl":"https://doi.org/10.21608/aprh.2019.12303.1083","url":null,"abstract":"Objectives: This study is aimed to be a comprehensive review of the phytochemical constituents and biological activities of Aizoaceae family plants (Mesembryanthemaceae). Methods: This study is covering articles between 1969 and 2018, reviewed from internationally accepted databases and scientific data from scientific Journals. Results: Phytochemically studied plants of family Aizoaceae have shown the presence of various classes of compounds including; alkaloids, triterpenes, sterols, lignans, phenolic compounds, betacyanins, and essential oils. Biological studies on plants of family Aizoaceae have indicated various bioactive potentials including antioxidant, antidiabetic, antimicrobial, antitumor, hepatoprotective, anti-inflammatory and other effects. The reported medicinal plants of family Aizoaceae were selected and summarized on the basis of their; phytochemical constituents and biological activities. Conclusion: The results of this study may inspire further ethno-botanical and ethno-pharmacological research and investigations toward drug discovery.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79888774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-10DOI: 10.21608/APRH.2019.13691.1084
M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag
Objectives: The present study aimed to investigate and isolate different phytochemical constituents and assess the antioxidant and antimicrobial activities of the total methanol extract and different fractions of Phagnalon barbeyanum aerial parts. Methods: Phytochemical screening of Phagnalon barbeyanum aerial parts was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different spectral techniques (1H NMR and 13C NMR). The antioxidant activity was evaluated by DPPH radical scavenging assay and antimicrobial activity was done by standard agar well diffusion assay. Results: Phytochemical investigation of Phagnalon barbeyanum aerial partswas done forthe presence ofcarbohydrates and or glycosides, sterols and or triterpene, flavonoids, tannins and saponins and revealed the absence of alkaloids and anthraquinones. Compounds; β-sitosterol (1), apigenin (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from methylene chloride and ethyl acetate fractions. The ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and methylene chloride fractions. The total methanol extract and n-hexane fractions showed lowest antioxidant activity. The ethyl acetate fraction and total methanol extract showed moderate activity against Staphylococcus aureus and Vancomycin resistant Staphylococcus aureus. Conclusion: The aerial parts of Phagnalon barbeyanum is a rich source of different classes of active constituents as phenolics. The total methanol extract of Phagnalon barbeyanum aerial parts and some of its fractionated concentrates could be considered as antioxidant and antimicrobial agents.
目的:研究和分离巴贝飞天部位总甲醇提取物和不同部位的不同化学成分，并评价其抗氧化和抗菌活性。方法:采用对每一类化合物进行特异性试验的方法，对巴贝飞天部位进行植物化学筛选。采用不同的色谱技术分离纯化化合物，并采用不同的光谱技术(1H NMR和13C NMR)对化合物结构进行了鉴定。用DPPH自由基清除法测定其抗氧化活性，用标准琼脂孔扩散法测定其抑菌活性。结果:对barbeyanum气相部分进行了植物化学分析，主要成分为碳水化合物和苷类、甾醇和三萜、黄酮类、单宁类和皂苷类，不含生物碱和蒽醌类。化合物;从二氯甲烷和乙酸乙酯中分离得到β-谷甾醇(1)、芹菜素(2)和β-谷甾醇-3- 0 -β- d -葡萄糖吡喃苷(3)。乙酸乙酯部位抗氧化活性最高，其次是正丁醇部位和二氯甲烷部位。总甲醇提取物和正己烷提取物的抗氧化活性最低。乙酸乙酯部分和总甲醇提取物对金黄色葡萄球菌和耐万古霉素金黄色葡萄球菌具有中等活性。结论:barbeyanphagnalon barbeyanum的地上部分含有丰富的酚类活性成分。barbeyanum地上部分总甲醇提取物及其部分分馏浓缩液可作为抗氧化和抗菌药物。
{"title":"Phytochemical Investigation and Assessment of Antioxidant and Antimicrobial Activities of Phagnalon barbeyanum Aerial Parts","authors":"M. R. Kamel, A. M. Nafady, A. Hassanein, R. Ragaey, E. Haggag","doi":"10.21608/APRH.2019.13691.1084","DOIUrl":"https://doi.org/10.21608/APRH.2019.13691.1084","url":null,"abstract":"Objectives: The present study aimed to investigate and isolate different phytochemical constituents and assess the antioxidant and antimicrobial activities of the total methanol extract and different fractions of Phagnalon barbeyanum aerial parts. Methods: Phytochemical screening of Phagnalon barbeyanum aerial parts was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different spectral techniques (1H NMR and 13C NMR). The antioxidant activity was evaluated by DPPH radical scavenging assay and antimicrobial activity was done by standard agar well diffusion assay. Results: Phytochemical investigation of Phagnalon barbeyanum aerial partswas done forthe presence ofcarbohydrates and or glycosides, sterols and or triterpene, flavonoids, tannins and saponins and revealed the absence of alkaloids and anthraquinones. Compounds; β-sitosterol (1), apigenin (2) and β-sitosterol-3-O-β-D- glucopyranoside (3) were isolated from methylene chloride and ethyl acetate fractions. The ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and methylene chloride fractions. The total methanol extract and n-hexane fractions showed lowest antioxidant activity. The ethyl acetate fraction and total methanol extract showed moderate activity against Staphylococcus aureus and Vancomycin resistant Staphylococcus aureus. Conclusion: The aerial parts of Phagnalon barbeyanum is a rich source of different classes of active constituents as phenolics. The total methanol extract of Phagnalon barbeyanum aerial parts and some of its fractionated concentrates could be considered as antioxidant and antimicrobial agents.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75644776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/APRH.2019.14036.1085
A. Haddad, W. Al-Shareef, S. Eid
Background: Nearly 50 % of the world residents are known to be infected with Helicobacter pylori which is the main cause of peptic ulcer and gastric cancer. Since resistance to the currently used treatments, other alternative approaches such as combination with natural products should be tried. Objectives: In this work, validated HPLC-UV method was developed for quantification of lepidine content in various extracts and crude oil of Lepidium sativum seeds. Furthermore, antibacterial activity of lepidine against H. pylori was compared with standard antibiotics supported by molecular docking study. Methods: Different solvents were used to extract lepidine as well as the contents were determined using HPLC-UV method. Anti-Helicobacter pylori activity was examined using agar diffusion and dilution methods. Molecular docking study was done on H. pylori phosphoribosyltransferase enzyme. Results: The highest content of lepidine (316.91 µg mL-1) was found in n-hexane extract. Lepidine exhibit antibacterialeffect (MIC = 6.25 µg mL-1) against H. pylori clinical isolates. The ability of lepidine to interact with amino acids in the phosphoribosyl transferase binding site might rationalize its observed activity. Conclusion: Our results demonstrate for the first time the anti-Helicobacter pylori activity of lepidine, which could therefore be developed as viable nutraceutical agent. Further investigations are required to formulate suitable pharmaceuticals to combat with the H. pylori infections on clinical grounds.
{"title":"Validated HPLC Determination of the Potential Anti-Helicobacter pylori, Lepidine, in Lepidium sativum Seeds Assessed by Molecular Docking Study","authors":"A. Haddad, W. Al-Shareef, S. Eid","doi":"10.21608/APRH.2019.14036.1085","DOIUrl":"https://doi.org/10.21608/APRH.2019.14036.1085","url":null,"abstract":"Background: Nearly 50 % of the world residents are known to be infected with Helicobacter pylori which is the main cause of peptic ulcer and gastric cancer. Since resistance to the currently used treatments, other alternative approaches such as combination with natural products should be tried. Objectives: In this work, validated HPLC-UV method was developed for quantification of lepidine content in various extracts and crude oil of Lepidium sativum seeds. Furthermore, antibacterial activity of lepidine against H. pylori was compared with standard antibiotics supported by molecular docking study. Methods: Different solvents were used to extract lepidine as well as the contents were determined using HPLC-UV method. Anti-Helicobacter pylori activity was examined using agar diffusion and dilution methods. Molecular docking study was done on H. pylori phosphoribosyltransferase enzyme. Results: The highest content of lepidine (316.91 µg mL-1) was found in n-hexane extract. Lepidine exhibit antibacterialeffect (MIC = 6.25 µg mL-1) against H. pylori clinical isolates. The ability of lepidine to interact with amino acids in the phosphoribosyl transferase binding site might rationalize its observed activity. Conclusion: Our results demonstrate for the first time the anti-Helicobacter pylori activity of lepidine, which could therefore be developed as viable nutraceutical agent. Further investigations are required to formulate suitable pharmaceuticals to combat with the H. pylori infections on clinical grounds.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77059629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/APRH.2019.10241.1080
Puspal De, M. Mukhopadhyay
Objectives: Turmeric is native to India and is widely used in Indian cuisine. Curcumin, the active principle of turmeric used primarily as a coloring agent in food, drug and cosmetics. The medicinal properties of curcumin are well known in ancient Indian Ayurvedic Medicine. Mitomycin C (MMC) is an antineoplastic agent used to fight a number of different cancers including cancer of the stomach, colon, rectum, pancreas, breast, lung, uterus, cervix, bladder, head, neck, eye and esophagus. It is a potent DNA cross-linker. But it has severe side effect, the prolonged use of the drug may result in permanent bone marrow damage and other various types of secondary tumors in normal cells. The present study was conducted to examine the anticlastogenic action of extracted curcumin from dry turmeric and pure curcumin against the MMC-induced chromosomal aberrations. For this purpose, in the present study, clastogenic parameter like chromosomal aberration test was conducted in peripheral human leukocytes. Methods: The antioxidant property of both extracted and pure curcumin was also evaluated by phosphomolybdenum method. Results: Our results demonstrated that, extracted as well as pure Curcumin, a strong antioxidant phyto-molecule is effective in counteracting the clastogenicity in human leukocyte in vitro. Conclusion: These results suggest that the use of turmeric in diet may be an effective protection against the health crisis generated by harmful agents.
{"title":"In Vitro Anticlastogenic and Antioxidant properties of extracted and Pure form of Curcumin against Mitomycin C in Human Peripheral Leukocytes","authors":"Puspal De, M. Mukhopadhyay","doi":"10.21608/APRH.2019.10241.1080","DOIUrl":"https://doi.org/10.21608/APRH.2019.10241.1080","url":null,"abstract":"Objectives: Turmeric is native to India and is widely used in Indian cuisine. Curcumin, the active principle of turmeric used primarily as a coloring agent in food, drug and cosmetics. The medicinal properties of curcumin are well known in ancient Indian Ayurvedic Medicine. Mitomycin C (MMC) is an antineoplastic agent used to fight a number of different cancers including cancer of the stomach, colon, rectum, pancreas, breast, lung, uterus, cervix, bladder, head, neck, eye and esophagus. It is a potent DNA cross-linker. But it has severe side effect, the prolonged use of the drug may result in permanent bone marrow damage and other various types of secondary tumors in normal cells. The present study was conducted to examine the anticlastogenic action of extracted curcumin from dry turmeric and pure curcumin against the MMC-induced chromosomal aberrations. For this purpose, in the present study, clastogenic parameter like chromosomal aberration test was conducted in peripheral human leukocytes. Methods: The antioxidant property of both extracted and pure curcumin was also evaluated by phosphomolybdenum method. Results: Our results demonstrated that, extracted as well as pure Curcumin, a strong antioxidant phyto-molecule is effective in counteracting the clastogenicity in human leukocyte in vitro. Conclusion: These results suggest that the use of turmeric in diet may be an effective protection against the health crisis generated by harmful agents.","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89415104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/aprh.2019.10458.1081
A. Hassan, H. el-Hifnawi, W. Ahmed
{"title":"Synthesis and Biological Evaluation of Certain α,β-Unsaturated Ketones and their Corresponding Fused Pyridines","authors":"A. Hassan, H. el-Hifnawi, W. Ahmed","doi":"10.21608/aprh.2019.10458.1081","DOIUrl":"https://doi.org/10.21608/aprh.2019.10458.1081","url":null,"abstract":"","PeriodicalId":15017,"journal":{"name":"Journal of Advanced Pharmacy Research","volume":"159 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91448397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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