Pub Date : 2024-06-20Epub Date: 2024-05-29DOI: 10.1128/jb.00059-24
N Luisa Hiller, Carlos J Orihuela
The major human pathogen Streptococcus pneumoniae has been the subject of intensive clinical and basic scientific study for over 140 years. In multiple instances, these efforts have resulted in major breakthroughs in our understanding of basic biological principles as well as fundamental tenets of bacterial pathogenesis, immunology, vaccinology, and genetics. Discoveries made with S. pneumoniae have led to multiple major public health victories that have saved the lives of millions. Studies on S. pneumoniae continue today, where this bacterium is being used to dissect the impact of the host on disease processes, as a powerful cell biology model, and to better understand the consequence of human actions on commensal bacteria at the population level. Herein we review the major findings, i.e., puzzle pieces, made with S. pneumoniae and how, over the years, they have come together to shape our understanding of this bacterium's biology and the practice of medicine and modern molecular biology.
{"title":"Biological puzzles solved by using <i>Streptococcus pneumoniae</i>: a historical review of the pneumococcal studies that have impacted medicine and shaped molecular bacteriology.","authors":"N Luisa Hiller, Carlos J Orihuela","doi":"10.1128/jb.00059-24","DOIUrl":"10.1128/jb.00059-24","url":null,"abstract":"<p><p>The major human pathogen <i>Streptococcus pneumoniae</i> has been the subject of intensive clinical and basic scientific study for over 140 years. In multiple instances, these efforts have resulted in major breakthroughs in our understanding of basic biological principles as well as fundamental tenets of bacterial pathogenesis, immunology, vaccinology, and genetics. Discoveries made with <i>S. pneumoniae</i> have led to multiple major public health victories that have saved the lives of millions. Studies on <i>S. pneumoniae</i> continue today, where this bacterium is being used to dissect the impact of the host on disease processes, as a powerful cell biology model, and to better understand the consequence of human actions on commensal bacteria at the population level. Herein we review the major findings, i.e., puzzle pieces, made with <i>S. pneumoniae</i> and how, over the years, they have come together to shape our understanding of this bacterium's biology and the practice of medicine and modern molecular biology.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-20Epub Date: 2024-05-10DOI: 10.1128/jb.00432-23
Belinda Candra, Deborah Cook, Janelle Hare
The DNA damage response of the multi-drug-resistant nosocomial pathogen Acinetobacter baumannii possesses multiple features that distinguish it from the commonly used LexA repression system. These include the absence of LexA in this genus, the evolution of a UmuD polymerase manager into the UmuDAb repressor of error-prone polymerases, the use of a corepressor unique to Acinetobacter (DdrR), and an unusually large UmuDAb binding site. We defined cis- and trans-acting factors required for UmuDAb DNA binding and gene repression, and tested whether DdrR directly enhances its DNA binding. We used DNA binding assays to characterize UmuDAb's binding to its proposed operator present upstream of the six co-repressed umuDC or umuC genes. UmuDAb bound tightly and cooperatively to this site with ~10-fold less affinity than LexA. DdrR enhanced the binding of both native and dimerization-deficient UmuDAb forms, but only in greater than equimolar ratios relative to UmuDAb. UmuDAb mutants unable to dimerize or effect gene repression showed impaired DNA binding, and a strain expressing the G124D dimerization mutant could not repress transcription of the UmuDAb-DdrR regulon. Competition electrophoretic mobility shift assays conducted with mutated operator probes showed that, unlike typical SOS boxes, the UmuDAb operator possessed a five-base pair central core whose sequence was more crucial for binding than the flanking palindrome. The presence of only one of the two flanking arms of the palindrome was necessary for UmuDAb binding. Overall, the data supported a model of an operator with two UmuDAb binding sites. The distinct characteristics of UmuDAb and its regulated promoters differ from the typical LexA repression model, demonstrating a novel method of repression.IMPORTANCEAcinetobacter baumannii is a gram-negative bacterium responsible for hospital-acquired infections. Its unique DNA damage response can activate multiple error-prone polymerase genes, allowing it to gain mutations that can increase its virulence and antibiotic resistance. The emergence of infectious strains carrying multiple antibiotic resistance genes, including carbapenem resistance, lends urgency to discovering and developing ways to combat infections resistant to treatment with known antibiotics. Deciphering how the regulators UmuDAb and DdrR repress the error-prone polymerases could lead to developing complementary treatments to halt this mechanism of generating resistance.
具有多种耐药性的医院病原体鲍曼不动杆菌的 DNA 损伤反应具有多种特征,有别于常用的 LexA 抑制系统。这些特征包括:该菌属中不存在 LexA、UmuD 聚合酶管理器进化为易出错聚合酶的 UmuDAb 抑制器、使用了鲍曼不动杆菌特有的核心抑制因子(DdrR)以及一个异常大的 UmuDAb 结合位点。我们定义了UmuDAb DNA结合和基因抑制所需的顺式和反式作用因子,并测试了DdrR是否能直接增强其DNA结合。我们使用DNA结合试验来鉴定UmuDAb与其存在于六个共同抑制的umuDC或umuC基因上游的拟议操作者的结合。UmuDAb与该位点紧密合作结合,其亲和力比LexA低约10倍。DdrR增强了原生和二聚化缺陷的UmuDAb形式的结合,但相对于UmuDAb而言,DdrR的结合比例高于等摩尔比例。不能二聚或产生基因抑制作用的UmuDAb突变体显示出DNA结合受损,表达G124D二聚突变体的菌株不能抑制UmuDAb-DdrR调控子的转录。用突变的操作子探针进行的竞争电泳迁移试验表明,与典型的SOS盒不同,UmuDAb操作子具有一个5碱基对的中心核心,其序列比侧翼的回文键对结合更为关键。在UmuDAb的结合过程中,只需存在两个侧翼回文臂中的一个。总之,这些数据支持一个具有两个 UmuDAb 结合位点的操作者模型。UmuDAb 及其调控启动子的独特特征不同于典型的 LexA 抑制模型,展示了一种新的抑制方法。重要意义鲍曼不动杆菌是一种革兰氏阴性细菌,是医院感染的罪魁祸首。其独特的 DNA 损伤反应可激活多个易出错的聚合酶基因,使其发生突变,从而增强毒性和抗生素耐药性。携带多种抗生素耐药性基因(包括碳青霉烯耐药性)的感染性菌株的出现,使得发现和开发抗击对已知抗生素治疗产生耐药性的感染的方法变得更加紧迫。破译调控因子UmuDAb和DdrR如何抑制易出错的聚合酶,有助于开发互补疗法,阻止这种产生抗药性的机制。
{"title":"Repression of <i>Acinetobacter baumannii</i> DNA damage response requires DdrR-assisted binding of UmuDAb dimers to atypical SOS box.","authors":"Belinda Candra, Deborah Cook, Janelle Hare","doi":"10.1128/jb.00432-23","DOIUrl":"10.1128/jb.00432-23","url":null,"abstract":"<p><p>The DNA damage response of the multi-drug-resistant nosocomial pathogen <i>Acinetobacter baumannii</i> possesses multiple features that distinguish it from the commonly used LexA repression system. These include the absence of LexA in this genus, the evolution of a UmuD polymerase manager into the UmuDAb repressor of error-prone polymerases, the use of a corepressor unique to <i>Acinetobacter</i> (DdrR), and an unusually large UmuDAb binding site. We defined cis- and trans-acting factors required for UmuDAb DNA binding and gene repression, and tested whether DdrR directly enhances its DNA binding. We used DNA binding assays to characterize UmuDAb's binding to its proposed operator present upstream of the six co-repressed <i>umuDC</i> or <i>umuC</i> genes. UmuDAb bound tightly and cooperatively to this site with ~10-fold less affinity than LexA. DdrR enhanced the binding of both native and dimerization-deficient UmuDAb forms, but only in greater than equimolar ratios relative to UmuDAb. UmuDAb mutants unable to dimerize or effect gene repression showed impaired DNA binding, and a strain expressing the G124D dimerization mutant could not repress transcription of the UmuDAb-DdrR regulon. Competition electrophoretic mobility shift assays conducted with mutated operator probes showed that, unlike typical SOS boxes, the UmuDAb operator possessed a five-base pair central core whose sequence was more crucial for binding than the flanking palindrome. The presence of only one of the two flanking arms of the palindrome was necessary for UmuDAb binding. Overall, the data supported a model of an operator with two UmuDAb binding sites. The distinct characteristics of UmuDAb and its regulated promoters differ from the typical LexA repression model, demonstrating a novel method of repression.IMPORTANCE<i>Acinetobacter baumannii</i> is a gram-negative bacterium responsible for hospital-acquired infections. Its unique DNA damage response can activate multiple error-prone polymerase genes, allowing it to gain mutations that can increase its virulence and antibiotic resistance. The emergence of infectious strains carrying multiple antibiotic resistance genes, including carbapenem resistance, lends urgency to discovering and developing ways to combat infections resistant to treatment with known antibiotics. Deciphering how the regulators UmuDAb and DdrR repress the error-prone polymerases could lead to developing complementary treatments to halt this mechanism of generating resistance.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-20Epub Date: 2024-05-31DOI: 10.1128/jb.00050-24
Roberto Kolter
While scientific research should be carried out objectively, the choices of questions asked and approaches taken are deeply personal and subjective. I urge individuals to pursue questions they love and to periodically scrutinize the reasons (the philosophies) that drive that love. As a case study, I scrutinize the "whys" behind some of the scientific questions I pursued during my career.
{"title":"Asking a question.","authors":"Roberto Kolter","doi":"10.1128/jb.00050-24","DOIUrl":"10.1128/jb.00050-24","url":null,"abstract":"<p><p>While scientific research should be carried out objectively, the choices of questions asked and approaches taken are deeply personal and subjective. I urge individuals to pursue questions they love and to periodically scrutinize the reasons (the philosophies) that drive that love. As a case study, I scrutinize the \"whys\" behind some of the scientific questions I pursued during my career.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jean-Paul Pirnay, Françoise Van Bambeke, Christine Baysse, Miguel Cámara, Sylvie Chevalier, Jean François Collet, Aurélie Crabbé, Jozef Dingemans, Alain Dufour, Linda Eeckhaudt, Alain Filloux, Olivier Lesouhaitier, Qing Wei, Daniel De Vos
{"title":"In memoriam: Pierre Cornelis (1949-2023).","authors":"Jean-Paul Pirnay, Françoise Van Bambeke, Christine Baysse, Miguel Cámara, Sylvie Chevalier, Jean François Collet, Aurélie Crabbé, Jozef Dingemans, Alain Dufour, Linda Eeckhaudt, Alain Filloux, Olivier Lesouhaitier, Qing Wei, Daniel De Vos","doi":"10.1128/jb.00179-24","DOIUrl":"https://doi.org/10.1128/jb.00179-24","url":null,"abstract":"","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
RsgA is a late-stage ribosome biogenesis factor. Earlier, infB (IF2) was isolated as a multicopy suppressor of the Escherichia coli ΔrsgA strain. How IF2 rescued the strain growth remained unclear. This study reveals that (i) the multicopy infB-mediated ...
{"title":"Lamotrigine-mediated rescue of RsgA-deficient Escherichia coli reveals another role of IF2 in ribosome biogenesis","authors":"Sudhir Singh, Jitendra Singh, Umesh Varshney","doi":"10.1128/jb.00119-24","DOIUrl":"https://doi.org/10.1128/jb.00119-24","url":null,"abstract":"RsgA is a late-stage ribosome biogenesis factor. Earlier, infB (IF2) was isolated as a multicopy suppressor of the Escherichia coli ΔrsgA strain. How IF2 rescued the strain growth remained unclear. This study reveals that\u0000(i) the multicopy infB-mediated ...","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141259663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23Epub Date: 2024-05-07DOI: 10.1128/jb.00048-24
Mei G Lei, Matthew A Jorgenson, Emily J Robbs, Ian M Black, Stephanie Archer-Hartmann, Sergei Shalygin, Parastoo Azadi, Chia Y Lee
Whole genome sequencing has revealed that the genome of Staphylococcus aureus possesses an uncharacterized 5-gene operon (SAOUHSC_00088-00092 in strain 8325 genome) that encodes factors with functions related to polysaccharide biosynthesis and export, indicating the existence of a new extracellular polysaccharide species. We designate this locus as ssc for staphylococcal surface carbohydrate. We found that the ssc genes were weakly expressed and highly repressed by the global regulator MgrA. To characterize Ssc, Ssc was heterologously expressed in Escherichia coli and extracted by heat treatment. Ssc was also conjugated to AcrA from Campylobacter jejuni in E. coli using protein glycan coupling technology (PGCT). Analysis of the heat-extracted Ssc and the purified Ssc-AcrA glycoconjugate by tandem mass spectrometry revealed that Ssc is likely a polymer consisting of N-acetylgalactosamine. We further demonstrated that the expression of the ssc genes in S. aureus affected phage adsorption and susceptibility, suggesting that Ssc is surface-exposed.
Importance: Surface polysaccharides play crucial roles in the biology and virulence of bacterial pathogens. Staphylococcus aureus produces four major types of polysaccharides that have been well-characterized. In this study, we identified a new surface polysaccharide containing N-acetylgalactosamine (GalNAc). This marks the first report of GalNAc-containing polysaccharide in S. aureus. Our discovery lays the groundwork for further investigations into the chemical structure, surface location, and role in pathogenesis of this new polysaccharide.
{"title":"Characterization of Ssc, an <i>N</i>-acetylgalactosamine-containing <i>Staphylococcus aureus</i> surface polysaccharide.","authors":"Mei G Lei, Matthew A Jorgenson, Emily J Robbs, Ian M Black, Stephanie Archer-Hartmann, Sergei Shalygin, Parastoo Azadi, Chia Y Lee","doi":"10.1128/jb.00048-24","DOIUrl":"10.1128/jb.00048-24","url":null,"abstract":"<p><p>Whole genome sequencing has revealed that the genome of <i>Staphylococcus aureus</i> possesses an uncharacterized 5-gene operon (SAOUHSC_00088-00092 in strain 8325 genome) that encodes factors with functions related to polysaccharide biosynthesis and export, indicating the existence of a new extracellular polysaccharide species. We designate this locus as <i>ssc</i> for staphylococcal surface carbohydrate. We found that the <i>ssc</i> genes were weakly expressed and highly repressed by the global regulator MgrA. To characterize Ssc, Ssc was heterologously expressed in <i>Escherichia coli</i> and extracted by heat treatment. Ssc was also conjugated to AcrA from <i>Campylobacter jejuni</i> in <i>E. coli</i> using protein glycan coupling technology (PGCT). Analysis of the heat-extracted Ssc and the purified Ssc-AcrA glycoconjugate by tandem mass spectrometry revealed that Ssc is likely a polymer consisting of <i>N</i>-acetylgalactosamine. We further demonstrated that the expression of the <i>ssc</i> genes in <i>S. aureus</i> affected phage adsorption and susceptibility, suggesting that Ssc is surface-exposed.</p><p><strong>Importance: </strong>Surface polysaccharides play crucial roles in the biology and virulence of bacterial pathogens. <i>Staphylococcus aureus</i> produces four major types of polysaccharides that have been well-characterized. In this study, we identified a new surface polysaccharide containing N-acetylgalactosamine (GalNAc). This marks the first report of GalNAc-containing polysaccharide in <i>S. aureus</i>. Our discovery lays the groundwork for further investigations into the chemical structure, surface location, and role in pathogenesis of this new polysaccharide.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-23Epub Date: 2024-05-07DOI: 10.1128/jb.00146-24
Natalia Kwiatos, Kevin J Waldron
A new study by Nies et al. (J Bacteriol 206:e00080-24, 2024, https://doi.org/10.1128/jb.00080-24) provides a rich, quantitative data set of zinc accumulation by cells of Cupriavidus metallidurans, including of mutant bacterial strains lacking import or efflux genes, and comparison of zinc accumulation by cells previously starved of metal with those of zinc-replete cells. The data surprisingly demonstrate the concomitant activity of both active metal import and metal efflux systems. They present a flow equilibrium model to describe zinc homeostasis in bacteria.
Nies 等人的一项新研究(J Bacteriol 206:e00080-24, 2024, https://doi.org/10.1128/jb.00080-24)提供了一组丰富的定量数据,说明了金属铜绿菌(Cupriavidus metallidurans)细胞的锌积累情况,包括缺乏导入或外排基因的突变菌株,并比较了先前缺乏金属的细胞与锌富集细胞的锌积累情况。这些数据令人惊讶地证明了活性金属导入和金属外排系统的同时活性。他们提出了一个流动平衡模型来描述细菌中的锌平衡。
{"title":"In a state of flux: new insight into the transport processes that maintain bacterial metal homeostasis.","authors":"Natalia Kwiatos, Kevin J Waldron","doi":"10.1128/jb.00146-24","DOIUrl":"10.1128/jb.00146-24","url":null,"abstract":"<p><p>A new study by Nies et al. (J Bacteriol 206:e00080-24, 2024, https://doi.org/10.1128/jb.00080-24) provides a rich, quantitative data set of zinc accumulation by cells of <i>Cupriavidus metallidurans</i>, including of mutant bacterial strains lacking import or efflux genes, and comparison of zinc accumulation by cells previously starved of metal with those of zinc-replete cells. The data surprisingly demonstrate the concomitant activity of both active metal import and metal efflux systems. They present a flow equilibrium model to describe zinc homeostasis in bacteria.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vicki MooreWim Vermaas1School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona, USA, Conrad W. Mullineaux
Journal of Bacteriology, Ahead of Print.
细菌学杂志》,提前出版。
{"title":"Functional consequences of modification of the photosystem I/photosystem II ratio in the cyanobacterium Synechocystis sp. PCC 6803","authors":"Vicki MooreWim Vermaas1School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona, USA, Conrad W. Mullineaux","doi":"10.1128/jb.00454-23","DOIUrl":"https://doi.org/10.1128/jb.00454-23","url":null,"abstract":"Journal of Bacteriology, Ahead of Print. <br/>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Life is thought to have evolved in the ocean before expanding to terrestrial habitats. It is no wonder then that marine bacteria share genes, physiology, functional pathways, and regulatory mechanisms with their terrestrial cousins. These pathways and processes represent deeply rooted, conserved strategies that have evolved over billions of years and adapted to new habitats as the Earth and its increasingly diverse host species changed over time.
{"title":"Lighting the way: how the Vibrio fischeri model microbe reveals the complexity of Earth’s “simplest” life forms","authors":"Alecia N. Septer, Karen L. Visick","doi":"10.1128/jb.00035-24","DOIUrl":"https://doi.org/10.1128/jb.00035-24","url":null,"abstract":"Life is thought to have evolved in the ocean before expanding to terrestrial habitats.\u0000It is no wonder then that marine bacteria share genes, physiology, functional pathways,\u0000and regulatory mechanisms with their terrestrial cousins. These pathways and processes\u0000represent deeply rooted, conserved strategies that have evolved over billions of years\u0000and adapted to new habitats as the Earth and its increasingly diverse host species\u0000changed over time.","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amelia K. SchmidtCaleb M. SchwartzkopfJulie D. PourtoisElizabeth B. BurgenerDominick R. FaithAlex JoyceTyrza LammaGeetha KumarPaul L. BollykyPatrick R. Secor1Division of Biological Sciences, University of Montana, Missoula, Montana, USA2Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA3Division of Pediatric Pulmonology and Sleep Medicine, Children’s Hospital of Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, California, USA4Center for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, California, USA5School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India, Joseph Bondy-Denomy
Journal of Bacteriology, Ahead of Print.
细菌学杂志》,提前出版。
{"title":"Targeted deletion of Pf prophages from diverse Pseudomonas aeruginosa isolates has differential impacts on quorum sensing and virulence traits","authors":"Amelia K. SchmidtCaleb M. SchwartzkopfJulie D. PourtoisElizabeth B. BurgenerDominick R. FaithAlex JoyceTyrza LammaGeetha KumarPaul L. BollykyPatrick R. Secor1Division of Biological Sciences, University of Montana, Missoula, Montana, USA2Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA3Division of Pediatric Pulmonology and Sleep Medicine, Children’s Hospital of Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, California, USA4Center for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, California, USA5School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India, Joseph Bondy-Denomy","doi":"10.1128/jb.00402-23","DOIUrl":"https://doi.org/10.1128/jb.00402-23","url":null,"abstract":"Journal of Bacteriology, Ahead of Print. <br/>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}