K. Kanon, Bushra Jannat, Shohei Komaki, M. Alam, M. Alam
The freshwater striped snakehead, Channa striata, is widely distributed in several Asian countries such as China, Vietnam, Indonesia, Malaysia, India, Myanmar, and Bangladesh. The present study investigated various C. striata populations sequentially by the mitochondrial cytochrome b gene. Native populations (136) were collected from nine geographically different locations and the Vietnamese populations (50) from two other fish farms in Bangladesh. The partial sequence of Cyt b (836bp) was analyzed, and the results have identified five haplotypes from nine natives and a single haplotype from two Vietnamese populations. This demonstrated that Vietnam-originated populations in Bangladesh had the same origin. In contrast, the pairwise highest Fst value observed between Native and Vietnamese populations shows a substantial genetic variation between them. The phylogenetic tree separated both the native and exotic populations, whereas five native haplotypes formed a cluster with the Indian C. striata. In contrast, the Vietnam haplotype formed a clade with the East Asian C. striata suggesting possible cryptic genetic diversity. Finally, the mitochondrial cytochrome b gene can also be used to identify native and Vietnam-originated C. striata strain available in Bangladesh, beneficial to broodstock development and conservation issues. Besides, a detailed morphological study of Vietnam-originated C. striata needs to be required to identify the exact morphotype available in Bangladesh.
{"title":"MOLECULAR DIFFERENTIATION BETWEEN NATIVE AND VIETNAM ORIGINATED STRIPED SNAKEHEADS (CHANNA STRIATA) IN BANGLADESH USING MITOCHONDRIAL CYTOCHROME B GENE","authors":"K. Kanon, Bushra Jannat, Shohei Komaki, M. Alam, M. Alam","doi":"10.5455/jbau.113332","DOIUrl":"https://doi.org/10.5455/jbau.113332","url":null,"abstract":"The freshwater striped snakehead, Channa striata, is widely distributed in several Asian countries such as China, Vietnam, Indonesia, Malaysia, India, Myanmar, and Bangladesh. The present study investigated various C. striata populations sequentially by the mitochondrial cytochrome b gene. Native populations (136) were collected from nine geographically different locations and the Vietnamese populations (50) from two other fish farms in Bangladesh. The partial sequence of Cyt b (836bp) was analyzed, and the results have identified five haplotypes from nine natives and a single haplotype from two Vietnamese populations. This demonstrated that Vietnam-originated populations in Bangladesh had the same origin. In contrast, the pairwise highest Fst value observed between Native and Vietnamese populations shows a substantial genetic variation between them. The phylogenetic tree separated both the native and exotic populations, whereas five native haplotypes formed a cluster with the Indian C. striata. In contrast, the Vietnam haplotype formed a clade with the East Asian C. striata suggesting possible cryptic genetic diversity. Finally, the mitochondrial cytochrome b gene can also be used to identify native and Vietnam-originated C. striata strain available in Bangladesh, beneficial to broodstock development and conservation issues. Besides, a detailed morphological study of Vietnam-originated C. striata needs to be required to identify the exact morphotype available in Bangladesh.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73721931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Roy, Abu N. M. A. Rahman, M. Kar, M. Hossen, M. Islam, U. Rima
In the present study, the effect of topical application of Aloe vera, honey and combination of Aloe vera and honey (A+H) on cutaneous wound healing in goat model was investigated. A total of 20 goats were selected having body weight 8-12 kg, age 9-18 months and randomly divided into group A, H, A+H and C. Following local anaesthesia, a 1.5×1.5 cm² excised wound along with the latissimus dorsi muscle were made on the skin of these goats using a template. The wounds of Group A, H, A+H and C were treated topically with Aloe vera, honey and Aloe vera + honey and sterile normal saline respectively. The efficacy of the treatment was evaluated in terms of bacterial load, tissue response in healing by histopathology and wound contraction rate. The bacterial load was measured by growing them on agar medium and means of colony forming unit (CFU). The wound area in each group was measured using a Vernier calliper scale which was used to trace the wound area. The bacterial load (CFU) appeared lower in every sampling in goats of Group H followed by those of Group A and A+H. Highest level of bacterial infectivity (1700-1980 cfu/100µl nutrient agar) in group C and lower count in Group H (250-362 /100 µl nutrient agar) was seen in the wound following day 3 of treatment. Biopsy examination of Group A skin revealed that the topical application of Aloe vera lowered tissue reaction following day 7 of wounding and enabling rapid repairing of tissue with complete epithelialization over the wound following 14 days of wounding. Clinically the wounds showed various degree of healing in group A, H, A+H goats following day 14 of wounding compared to the existence of raw and unhealed wound in Group C goats. Complete centripetal contraction of wound was obtained in group A, A+H, H at 17, 19, 21 days respectively. The results reveal that topical application of Aloe vera alone appeared best in the wound healing process followed by Aloe vera + honey and honey respectively.
{"title":"Evaluation of Topical Application of Aloe vera gel and Honey for Acceleration of Cutaneous Wound Healing in Goat Model","authors":"K. Roy, Abu N. M. A. Rahman, M. Kar, M. Hossen, M. Islam, U. Rima","doi":"10.5455/jbau.132070","DOIUrl":"https://doi.org/10.5455/jbau.132070","url":null,"abstract":"In the present study, the effect of topical application of Aloe vera, honey and combination of Aloe vera and honey (A+H) on cutaneous wound healing in goat model was investigated. A total of 20 goats were selected having body weight 8-12 kg, age 9-18 months and randomly divided into group A, H, A+H and C. Following local anaesthesia, a 1.5×1.5 cm² excised wound along with the latissimus dorsi muscle were made on the skin of these goats using a template. The wounds of Group A, H, A+H and C were treated topically with Aloe vera, honey and Aloe vera + honey and sterile normal saline respectively. The efficacy of the treatment was evaluated in terms of bacterial load, tissue response in healing by histopathology and wound contraction rate. The bacterial load was measured by growing them on agar medium and means of colony forming unit (CFU). The wound area in each group was measured using a Vernier calliper scale which was used to trace the wound area. The bacterial load (CFU) appeared lower in every sampling in goats of Group H followed by those of Group A and A+H. Highest level of bacterial infectivity (1700-1980 cfu/100µl nutrient agar) in group C and lower count in Group H (250-362 /100 µl nutrient agar) was seen in the wound following day 3 of treatment. Biopsy examination of Group A skin revealed that the topical application of Aloe vera lowered tissue reaction following day 7 of wounding and enabling rapid repairing of tissue with complete epithelialization over the wound following 14 days of wounding. Clinically the wounds showed various degree of healing in group A, H, A+H goats following day 14 of wounding compared to the existence of raw and unhealed wound in Group C goats. Complete centripetal contraction of wound was obtained in group A, A+H, H at 17, 19, 21 days respectively. The results reveal that topical application of Aloe vera alone appeared best in the wound healing process followed by Aloe vera + honey and honey respectively.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78328130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was conducted to biofortify Fe in tomato fruit. Seven tomato varieties were tested in this study to screen out the potential variety for biofortification. Based on Fe concentrations of tomato fruit, BARI Tomato-14 was selected as test crop. Six different methods for Fe application viz. 100% as seedling priming, 50% in soil + 50% as foliar spray at seedling stage, 50% as seedling priming + 50% as foliar spray, 100% as foliar spray at seedling stage, 100% as foliar spray at flowering stage and 100% as foliar spray at fruiting stage @ 4 kg Fe ha-1. Iron was applied for 3 times at 7 day interval at all growth stages. Experiments were laid out in a completely randomized design (CRD) with 3 replications. Different application methods of Fe significantly influenced the growth, yield contributing characteristics, yield, biochemical constituents, nutrient concentrations and their uptake by BARI Tomato-14. The highest values of most of the studied parameters including Fe content and uptake were recorded from the application of 50% Fe as seedling priming + 50% Fe as foliar spray. Significant and positive correlations among the growth and yield contributing parameters due to the application of Fe were observed. Interestingly the highest vitamin-C, protein, lycopene, N, K and Ca contents and uptake were observed in 50% as seedling priming + 50% as foliar spray of Fe @ 4 kg ha-1. Except few most of the studied nutrients were negatively correlated with Fe. Iron was biofortified by 66.28 µg g-1 in BARI Tomato-14. Results suggest that application of 2 kg Fe ha-1 as seedling priming + 2 kg Fe ha-1 as foliar spray at seedling stage for 3 times at 7 days interval along with the recommended doses of NPK fertilizers in soil can be practiced for Fe biofortification in tomato.
对番茄果实中的铁进行了生物强化研究。本研究对7个番茄品种进行了试验，以筛选潜在的生物强化品种。根据番茄果实铁浓度，选择BARI番茄-14作为试验作物。六种不同的铁施用方法，即100%引苗，50%土壤+ 50%苗期叶面喷雾，50%苗期引苗+ 50%叶面喷雾，苗期100%叶面喷雾，开花期100%叶面喷雾，结果期100%叶面喷雾@ 4 kg Fe hm -1。各生育期每隔7 d施用3次铁。试验采用完全随机设计(CRD)， 3个重复。不同施铁方式对BARI番茄14的生长、产量贡献特性、产量、生化成分、养分浓度及其吸收均有显著影响。苗期喷施50%铁+叶面喷施50%铁处理的铁含量和吸收量均最高。结果表明，铁的施用对水稻生长和产量的影响呈显著正相关。苗期引种50% +叶面喷施Fe @ 4 kg hm -1 50%时，维生素c、蛋白质、番茄红素、N、K、Ca含量和吸收量最高。除少数元素外，大部分营养元素与铁呈负相关。BARI Tomato-14的铁含量为66.28µg g-1。结果表明，苗期喷施2 kg Fe ha-1 + 2 kg Fe ha-1，每隔7 d喷施3次，配合土壤氮磷钾推荐用量，可实现番茄铁生物强化。
{"title":"Agronomic approaches to biofortify iron in tomato","authors":"R. Rabbi, M. Chowdhury, B. Saha","doi":"10.5455/jbau.120912","DOIUrl":"https://doi.org/10.5455/jbau.120912","url":null,"abstract":"The study was conducted to biofortify Fe in tomato fruit. Seven tomato varieties were tested in this study to screen out the potential variety for biofortification. Based on Fe concentrations of tomato fruit, BARI Tomato-14 was selected as test crop. Six different methods for Fe application viz. 100% as seedling priming, 50% in soil + 50% as foliar spray at seedling stage, 50% as seedling priming + 50% as foliar spray, 100% as foliar spray at seedling stage, 100% as foliar spray at flowering stage and 100% as foliar spray at fruiting stage @ 4 kg Fe ha-1. Iron was applied for 3 times at 7 day interval at all growth stages. Experiments were laid out in a completely randomized design (CRD) with 3 replications. Different application methods of Fe significantly influenced the growth, yield contributing characteristics, yield, biochemical constituents, nutrient concentrations and their uptake by BARI Tomato-14. The highest values of most of the studied parameters including Fe content and uptake were recorded from the application of 50% Fe as seedling priming + 50% Fe as foliar spray. Significant and positive correlations among the growth and yield contributing parameters due to the application of Fe were observed. Interestingly the highest vitamin-C, protein, lycopene, N, K and Ca contents and uptake were observed in 50% as seedling priming + 50% as foliar spray of Fe @ 4 kg ha-1. Except few most of the studied nutrients were negatively correlated with Fe. Iron was biofortified by 66.28 µg g-1 in BARI Tomato-14. Results suggest that application of 2 kg Fe ha-1 as seedling priming + 2 kg Fe ha-1 as foliar spray at seedling stage for 3 times at 7 days interval along with the recommended doses of NPK fertilizers in soil can be practiced for Fe biofortification in tomato.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76199784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sadia Shupta, S. Chakraborty, Shimul Akhtar, M. Khokon
{"title":"Improvement of bacterial wilt tolerance in eggplants by endotrophic mycorrhiza (Glomus mosseae)","authors":"Sadia Shupta, S. Chakraborty, Shimul Akhtar, M. Khokon","doi":"10.5455/jbau.130804","DOIUrl":"https://doi.org/10.5455/jbau.130804","url":null,"abstract":"","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85137151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Sohel Rahman, M. Mojid, F. Yasmine, T. Ahmed
Application of fresh water at the salinity-sensitive growth stage(s) and saline water of tolerable salinity level at the less sensitive stage(s) may be an effective irrigation technique for rice (Oryza sativa L.) cultivation in fresh water-scarce salt-affected areas. However, implementation of this technique requires adequate prior information on the sensitivity of growth stages of the rice cultivars to salinity of irrigation water. The objective of this study was therefore to identify the sensitivity of growth stages of Binadhan-8, Binadhan-10, BRRI dhan28 and BRRI dhan47 to salt stress by irrigating the crops with fresh water (control), and saline water of 6, 9 and 12 dS m1 at establishment, tillering, panicle formation, flowering, maturity and all growth stages of the crops. The experiment was laid out in a split-split pot arrangement following a completely randomized design (CRD) with three replications. The establishment and flowering stages of the rice cultivars were found to be the most sensitive to salt stress. Therefore, irrigation at these growth stages with fresh water and at the other growth stages with saline water would be an appropriate strategy for cultivation of the tested rice cultivars in fresh water-scarce saline areas like the coastal saline region of Bangladesh.
{"title":"Growth-stage-sensitivity of four HYV rice cultivars to irrigation water salinity","authors":"Mohammad Sohel Rahman, M. Mojid, F. Yasmine, T. Ahmed","doi":"10.5455/jbau.38522","DOIUrl":"https://doi.org/10.5455/jbau.38522","url":null,"abstract":"Application of fresh water at the salinity-sensitive growth stage(s) and saline water of tolerable salinity level at the less sensitive stage(s) may be an effective irrigation technique for rice (Oryza sativa L.) cultivation in fresh water-scarce salt-affected areas. However, implementation of this technique requires adequate prior information on the sensitivity of growth stages of the rice cultivars to salinity of irrigation water. The objective of this study was therefore to identify the sensitivity of growth stages of Binadhan-8, Binadhan-10, BRRI dhan28 and BRRI dhan47 to salt stress by irrigating the crops with fresh water (control), and saline water of 6, 9 and 12 dS m1 at establishment, tillering, panicle formation, flowering, maturity and all growth stages of the crops. The experiment was laid out in a split-split pot arrangement following a completely randomized design (CRD) with three replications. The establishment and flowering stages of the rice cultivars were found to be the most sensitive to salt stress. Therefore, irrigation at these growth stages with fresh water and at the other growth stages with saline water would be an appropriate strategy for cultivation of the tested rice cultivars in fresh water-scarce saline areas like the coastal saline region of Bangladesh.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90158655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nihad, Amirul Kabir, M. Rashid, Omma Honey, Mohammad Khan, Mohammad Latif
DNA extraction from rice leaves was developed earlier but a quick and cost-efficient DNA extraction method is still in search to conduct molecular research swiftly. Four DNA extraction methods i.e., extraction buffer (E1), K-acetate (E2) and two modified CTAB (E3 and E4) based protocols were tested for DNA concentration, purity of the extracted DNA, time and cost required per sample. Moreover, a Mixer mill (Mi; electrical method) and a Mortar and pestle (M; manual method) were used to test the performance of the four methods. DNA concentrations are insignificant among the methods MiE1 (266.61 ng/µL), MiE3 (260.58 ng/µL) and MiE4 (245.49 ng/µL) but these are all significantly different from the method MiE2 (137.13 ng/µL). ME2 required the maximum time (59 minutes), followed by ME1 (55 minutes), ME3 (40 minutes), and ME4 (21 minutes). In terms of cost, MiE3 and MiE4 required the highest (356.39 taka (4.19 dollars)/100 samples) amount of cost, whereas the lowest price (101.81 taka (1.20 dollars)/100 samples) was required in MiE2. For Purity-1 (260/280) (PU-1), no significant difference was found among the methods, but for Purity-2 (260/230) (PU-2), MiE2 significantly differed from the other methods. Through the mortar and pestle, the highest DNA concentration was found in ME1 (382.68 ng/µL) which significantly differed from ME2, ME3 and ME4. The highest times required in ME2 (59 min) followed by ME1 (55 min), ME3 (40 min) and ME4 (21 min) methods. The highest costs (356.39 taka (4.19 dollars)/100 samples) were required for ME3 and ME4 methods followed by ME2 (121.83 taka (1.43 dollars)/100 samples) and ME1 (101.81 taka (1.20 dollars)/100 samples). No significant difference was found among the methods for PU-1 but for PU-2, ME2 significantly differed from other methods. CTAB-based methods are the best and quick for DNA extraction but these methods are costly, whereas the other two methods are cheaper but they are time-consuming compared to CTAB.
{"title":"Comparative assessment of quick and cost-efficient quality DNA extraction methods from rice leaves","authors":"S. Nihad, Amirul Kabir, M. Rashid, Omma Honey, Mohammad Khan, Mohammad Latif","doi":"10.5455/jbau.107010","DOIUrl":"https://doi.org/10.5455/jbau.107010","url":null,"abstract":"DNA extraction from rice leaves was developed earlier but a quick and cost-efficient DNA extraction method is still in search to conduct molecular research swiftly. Four DNA extraction methods i.e., extraction buffer (E1), K-acetate (E2) and two modified CTAB (E3 and E4) based protocols were tested for DNA concentration, purity of the extracted DNA, time and cost required per sample. Moreover, a Mixer mill (Mi; electrical method) and a Mortar and pestle (M; manual method) were used to test the performance of the four methods. DNA concentrations are insignificant among the methods MiE1 (266.61 ng/µL), MiE3 (260.58 ng/µL) and MiE4 (245.49 ng/µL) but these are all significantly different from the method MiE2 (137.13 ng/µL). ME2 required the maximum time (59 minutes), followed by ME1 (55 minutes), ME3 (40 minutes), and ME4 (21 minutes). In terms of cost, MiE3 and MiE4 required the highest (356.39 taka (4.19 dollars)/100 samples) amount of cost, whereas the lowest price (101.81 taka (1.20 dollars)/100 samples) was required in MiE2. For Purity-1 (260/280) (PU-1), no significant difference was found among the methods, but for Purity-2 (260/230) (PU-2), MiE2 significantly differed from the other methods. Through the mortar and pestle, the highest DNA concentration was found in ME1 (382.68 ng/µL) which significantly differed from ME2, ME3 and ME4. The highest times required in ME2 (59 min) followed by ME1 (55 min), ME3 (40 min) and ME4 (21 min) methods. The highest costs (356.39 taka (4.19 dollars)/100 samples) were required for ME3 and ME4 methods followed by ME2 (121.83 taka (1.43 dollars)/100 samples) and ME1 (101.81 taka (1.20 dollars)/100 samples). No significant difference was found among the methods for PU-1 but for PU-2, ME2 significantly differed from other methods. CTAB-based methods are the best and quick for DNA extraction but these methods are costly, whereas the other two methods are cheaper but they are time-consuming compared to CTAB.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"140 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75695302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hosain, Md. Mizanur Rahman, M. Nuruzzaman, M. Munshi, A. Bari
{"title":"Morpho-physiological responses of rice to salicylic acid under drought stress","authors":"M. Hosain, Md. Mizanur Rahman, M. Nuruzzaman, M. Munshi, A. Bari","doi":"10.5455/jbau.16004","DOIUrl":"https://doi.org/10.5455/jbau.16004","url":null,"abstract":"","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80481452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Chowdhury, M. Mamun, Aliar Rahman, Amirul Hoque
In the present study, the effects of phytase supplementation with and without citric acid (CA) on growth performance, carcass characteristics and serum minerals concentration, tibia and shank characteristics, and phosphorus (P) retention were determined in broilers fed non-phytate P deficient diets. Ninety six one day old male broilers (COBB-500) were divided into four groups (twenty four birds each, and were fed one of the following diets until they were 35 days old: positive control (PC) diet formulated based on the NRC (1994) recommendations, negative control (NC) diet containing 0.20 % lower non-phytate P than that in the PC diet, and two other diets were formulated by adding only phytase (500 FTU/kg of feed) or phytase with CA (500 FTU/kg of feed with 2 % CA). At the age of 36 day, birds were sacrificed, samples were collected, processed, and then analyzed. Compared with the PC group, NC group showed impaired growth performance (final BW, BW gain, and FCR), serum P concentration, tibia and shank characteristics (dry weight, contents of ash and P). However, in most cases, these impairments were ameliorated (P<0.05) by the addition of phytase, and the restoration magnitude was non-significantly greater in phytase with CA group. Retention of total P increased (P<0.05) in phytase added groups compared with NC group, and was comparable with PC group, although non-phytate P level was lower in those groups. In conclusion, broilers fed phytase added non-phytate P deficient diets (without any dietary synthetic source of P) showed the growth performance, mineral (P) concentration in blood and bone, and relative retention of P comparable with broilers fed recommended one. Addition of CA along with phytase in diet was a costlier affair without any significant beneficial effects.
{"title":"Phosphorus availability in broilers given phosphorus deficient diets containing phytase with and without citric acid","authors":"R. Chowdhury, M. Mamun, Aliar Rahman, Amirul Hoque","doi":"10.5455/jbau.50128","DOIUrl":"https://doi.org/10.5455/jbau.50128","url":null,"abstract":"In the present study, the effects of phytase supplementation with and without citric acid (CA) on growth performance, carcass characteristics and serum minerals concentration, tibia and shank characteristics, and phosphorus (P) retention were determined in broilers fed non-phytate P deficient diets. Ninety six one day old male broilers (COBB-500) were divided into four groups (twenty four birds each, and were fed one of the following diets until they were 35 days old: positive control (PC) diet formulated based on the NRC (1994) recommendations, negative control (NC) diet containing 0.20 % lower non-phytate P than that in the PC diet, and two other diets were formulated by adding only phytase (500 FTU/kg of feed) or phytase with CA (500 FTU/kg of feed with 2 % CA). At the age of 36 day, birds were sacrificed, samples were collected, processed, and then analyzed. Compared with the PC group, NC group showed impaired growth performance (final BW, BW gain, and FCR), serum P concentration, tibia and shank characteristics (dry weight, contents of ash and P). However, in most cases, these impairments were ameliorated (P<0.05) by the addition of phytase, and the restoration magnitude was non-significantly greater in phytase with CA group. Retention of total P increased (P<0.05) in phytase added groups compared with NC group, and was comparable with PC group, although non-phytate P level was lower in those groups. In conclusion, broilers fed phytase added non-phytate P deficient diets (without any dietary synthetic source of P) showed the growth performance, mineral (P) concentration in blood and bone, and relative retention of P comparable with broilers fed recommended one. Addition of CA along with phytase in diet was a costlier affair without any significant beneficial effects.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84899980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although the same pyrethroid insecticide is impregnated in smoke and non-smoke coils, the non-smoke one emits fewer PICs (products of incomplete combustion) due to its different base materials, hence gaining popularity among the enlightened. However, the impacts of non-smoke mosquito coils on the respiratory and male reproductive systems in an insufficiently ventilated room simulating human’s closed bedrooms is yet to reveal. In this experiment, a total of eighteen male rats (age: 10-12 weeks) were randomly divided into three groups, each group containing six animals. The control group was exposed to normal environmental air, the non-smoke coil group inhaled D-transallethrin based coil smoke in a closed atmosphere and the smoke group was exposed to meperfluthrin based coil smoke in a well-ventilated room, for 8 hours per night. After 4 weeks of exposure, sperm parameters as well as testicular and pulmonary histopathology were accessed. The findings indicated that both mosquito coils considerably deteriorated sperm quality along with testicular and pulmonary architecture, with little distinction between them. Furthermore, the degenerated seminiferous tubules and distorted respiratory bronchioles lead us to conclude that if the room is not well-ventilated, the pollutants produced by burning non-smoke mosquito coils greatly exceed and it becomes as deleterious as conventional smoke emitting ones for the respiratory and male reproductive system.
{"title":"Hazardous effects of smoke and smokeless mosquito coils on the fertility and respiratory functions in rats","authors":"M. Amin","doi":"10.5455/jbau.113130","DOIUrl":"https://doi.org/10.5455/jbau.113130","url":null,"abstract":"Although the same pyrethroid insecticide is impregnated in smoke and non-smoke coils, the non-smoke one emits fewer PICs (products of incomplete combustion) due to its different base materials, hence gaining popularity among the enlightened. However, the impacts of non-smoke mosquito coils on the respiratory and male reproductive systems in an insufficiently ventilated room simulating human’s closed bedrooms is yet to reveal. In this experiment, a total of eighteen male rats (age: 10-12 weeks) were randomly divided into three groups, each group containing six animals. The control group was exposed to normal environmental air, the non-smoke coil group inhaled D-transallethrin based coil smoke in a closed atmosphere and the smoke group was exposed to meperfluthrin based coil smoke in a well-ventilated room, for 8 hours per night. After 4 weeks of exposure, sperm parameters as well as testicular and pulmonary histopathology were accessed. The findings indicated that both mosquito coils considerably deteriorated sperm quality along with testicular and pulmonary architecture, with little distinction between them. Furthermore, the degenerated seminiferous tubules and distorted respiratory bronchioles lead us to conclude that if the room is not well-ventilated, the pollutants produced by burning non-smoke mosquito coils greatly exceed and it becomes as deleterious as conventional smoke emitting ones for the respiratory and male reproductive system.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83816266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sadiya Juthee, M. Karim, M. Polash, M. Fakir, M. Hossain
Plant extract has been exploited for biogenic synthesis of metallic nanoparticles. This is considered as the promising alternative routes of chemical and physical synthesis methods owing to their abundancy in nature and ecofriendly friendly synthesis protocol. Rose is the top-ranked and universally favorite cut flower. Poor post-harvest management deteriorates the quality and reduces the vase life. Microbial proliferation of the stem base in the vase solution is a concern for these cut flowers that shortens their vase-life. To overcome this problem, a smart solution i.e. phytofabricated silver nanoparticles have been shown to act as anti-microbial agent. In this study, leaf extracts of Camellia sinensis were exploited to fabricate biogenic silver nanoparticles which showing UV-peak absorption ranging from 412-500 nm. This biogenic silver nanoparticles were applied @ 0.01, 0.05 and 0.1mM and compared with a control (without AgNPs) and silver nitrate (AgNO3). Interestingly, AgNPs showed a strong antimicrobial activity in vase solution and cut roses extended their vase life up to 13 days compared to 8 days in control and 9 days in AgNO3. Statistical differences in flower opening, bacterial growth (CFUmL−1) in vase solutions, water uptake, relative fresh weight and vase life of cut roses were found among treatments. In vitro microbial analysis and microscopic investigation of vessels showed that the development of microorganisms was reduced by a high concentration of AgNPs (0.1mM) at the cut end of flower and improving water uptake that followed by extended flowers vase life. The unique phytofabricated AgNPs technology can serve as a promising preservative to increase the ornamental value of cut rose flowers. Taken all together, applied phytofabricated AgNPs in vase solution significantly enhance vase life of cut rose flowers over control.
{"title":"Phytofabricated Silver Nanoparticles (AgNPs) Applied in Vase Solution as a Novel Anti-Microbial Agent for Enhancing the Vase-life of Cut-Rose Flower","authors":"Sadiya Juthee, M. Karim, M. Polash, M. Fakir, M. Hossain","doi":"10.5455/jbau.140222","DOIUrl":"https://doi.org/10.5455/jbau.140222","url":null,"abstract":"Plant extract has been exploited for biogenic synthesis of metallic nanoparticles. This is considered as the promising alternative routes of chemical and physical synthesis methods owing to their abundancy in nature and ecofriendly friendly synthesis protocol. Rose is the top-ranked and universally favorite cut flower. Poor post-harvest management deteriorates the quality and reduces the vase life. Microbial proliferation of the stem base in the vase solution is a concern for these cut flowers that shortens their vase-life. To overcome this problem, a smart solution i.e. phytofabricated silver nanoparticles have been shown to act as anti-microbial agent. In this study, leaf extracts of Camellia sinensis were exploited to fabricate biogenic silver nanoparticles which showing UV-peak absorption ranging from 412-500 nm. This biogenic silver nanoparticles were applied @ 0.01, 0.05 and 0.1mM and compared with a control (without AgNPs) and silver nitrate (AgNO3). Interestingly, AgNPs showed a strong antimicrobial activity in vase solution and cut roses extended their vase life up to 13 days compared to 8 days in control and 9 days in AgNO3. Statistical differences in flower opening, bacterial growth (CFUmL−1) in vase solutions, water uptake, relative fresh weight and vase life of cut roses were found among treatments. In vitro microbial analysis and microscopic investigation of vessels showed that the development of microorganisms was reduced by a high concentration of AgNPs (0.1mM) at the cut end of flower and improving water uptake that followed by extended flowers vase life. The unique phytofabricated AgNPs technology can serve as a promising preservative to increase the ornamental value of cut rose flowers. Taken all together, applied phytofabricated AgNPs in vase solution significantly enhance vase life of cut rose flowers over control.","PeriodicalId":15283,"journal":{"name":"Journal of Bangladesh Agricultural University","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82793632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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