S. Yoo, ChinJong-eon ChinJong-eon, Sung-Hoon Oh, Min Jung Ryu, Kwontack Hwang, Min-sung Lee, Hae-suk Jung
{"title":"A Study on the Evaluation of Distribution Stability of Refrigerated Rice Cake Using Natural Antibiotics","authors":"S. Yoo, ChinJong-eon ChinJong-eon, Sung-Hoon Oh, Min Jung Ryu, Kwontack Hwang, Min-sung Lee, Hae-suk Jung","doi":"10.17642/JCC.23.4.5","DOIUrl":"https://doi.org/10.17642/JCC.23.4.5","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76745432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amyloid plaque is a product of aggregation of β-amyloid peptide (Aβ) and is an important factor in the pathogenesis of Alzheimer’s Disease (AD). Aβ is a major component of amyloid plaque and vascular deposits in the AD brain. The enzyme β- secretase is required for the production of Aβ; thus, prevention of the formation of Aβ through the inhibition of β-secretase is a major focus in the study of the treatment of AD. In this study, we investigated β-secretase inhibitory activity of an Arctoscopus japonicus peptide. An Alcalase hydrolysate had the highest β-secretase inhibitory activity. A β-secretase inhibitory activity peptide was separated using ion exchange column chromatography (carboxy-methyl: CM, quaternary methyl ammonium: QMA) and reverse phase high performance liquid chromatography (RP-HPLC) on a C18 column. The IC50 value of the purified peptide was 248.2±1.73 μg/mL. The β-secretase inhibitory peptide was identified as a six amino acid residue of Gly-Pro-Val-Gly-Ala- Pro (MW: 497.27 Da). In cell viability experiments, the final purified fraction, the carboxy-methyl ion exchange column fraction (CM-F1) showed no significant cytotoxic effect in SH-SY5Y cells at concentrations below 100 μg/mL in 24 h. The results of this study suggest that peptides separated from Arctoscopus japonicus may be beneficial as β-secretase inhibitor compounds in functional foods.
{"title":"Characterization of a New Anti-dementia β-secretase Inhibitory Peptide from Arctoscopus japonicus","authors":"Seul Bit Na Park, S. Kim, H. Byun","doi":"10.17642/jcc.23.4.2","DOIUrl":"https://doi.org/10.17642/jcc.23.4.2","url":null,"abstract":"Amyloid plaque is a product of aggregation of β-amyloid peptide (Aβ) and is an important factor in the pathogenesis of Alzheimer’s Disease (AD). Aβ is a major component of amyloid plaque and vascular deposits in the AD brain. The enzyme β- secretase is required for the production of Aβ; thus, prevention of the formation of Aβ through the inhibition of β-secretase is a major focus in the study of the treatment of AD. In this study, we investigated β-secretase inhibitory activity of an Arctoscopus japonicus peptide. An Alcalase hydrolysate had the highest β-secretase inhibitory activity. A β-secretase inhibitory activity peptide was separated using ion exchange column chromatography (carboxy-methyl: CM, quaternary methyl ammonium: QMA) and reverse phase high performance liquid chromatography (RP-HPLC) on a C18 column. The IC50 value of the purified peptide was 248.2±1.73 μg/mL. The β-secretase inhibitory peptide was identified as a six amino acid residue of Gly-Pro-Val-Gly-Ala- Pro (MW: 497.27 Da). In cell viability experiments, the final purified fraction, the carboxy-methyl ion exchange column fraction (CM-F1) showed no significant cytotoxic effect in SH-SY5Y cells at concentrations below 100 μg/mL in 24 h. The results of this study suggest that peptides separated from Arctoscopus japonicus may be beneficial as β-secretase inhibitor compounds in functional foods.","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74146166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improving Smoothness of Hydrophilic Natural Polymer Coating Layer by Optimizing Composition of Coating Solution and Modifying Chemical Properties of Cobalt-Chrome Stent Surface","authors":"KimDae Hwan KimDae Hwan, C. Kum","doi":"10.17642/JCC.23.4.6","DOIUrl":"https://doi.org/10.17642/JCC.23.4.6","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85063791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spray-Dried Powder Preparation and Physicochemical Properties of Green Coffee Bean Extracts","authors":"Ju-Hyeon Kim, Joo-Heon Hong","doi":"10.17642/JCC.23.4.4","DOIUrl":"https://doi.org/10.17642/JCC.23.4.4","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"137 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74682004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ju-ryun Na, Eun Kim, Soyi Park, Ki Hoon Lee, Eui-seon Jeong, Jin-Seok Kim, YoungJae Kim, Sunoh Kim
{"title":"Eliminatory Effect of Mixture including Hot Water Extract of Dendropanax morbifera Lev. on Alcohol-induced Blood Alcohol Concentration and Hangover in Rat","authors":"Ju-ryun Na, Eun Kim, Soyi Park, Ki Hoon Lee, Eui-seon Jeong, Jin-Seok Kim, YoungJae Kim, Sunoh Kim","doi":"10.17642/JCC.23.4.8","DOIUrl":"https://doi.org/10.17642/JCC.23.4.8","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"249 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72957648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on the Sugar Analysis and Biological Activity of Sap from Juglans mandshurica Maxim","authors":"KimDae-wook KimDae-wook, ChoiMi-na ChoiMi-na, Min-sung Lee, Hae-suk Jung, Jun-Gi Byeon, Yeong-Su Kim","doi":"10.17642/JCC.23.4.7","DOIUrl":"https://doi.org/10.17642/JCC.23.4.7","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85597000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of Cellulase from Bacillus subtilis NSC Isolated from Soil","authors":"S. J. Kim, Chang-Su Park","doi":"10.17642/JCC.23.4.3","DOIUrl":"https://doi.org/10.17642/JCC.23.4.3","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86728244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant Activity of Extracellular Polysaccharides (EPS) from Marine Microalga Tetraselmis tertiolecta Depending upon their Molecular Weight","authors":"S. Amna, A. Yoo, Jae Kweon Park","doi":"10.17642/JCC.23.3.6","DOIUrl":"https://doi.org/10.17642/JCC.23.3.6","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88901002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Fuentes, N. Balagurusamy, Carolina E. Vita, R. Hours, Cristóbal N. Aguilar, S. Cavalitto
Aspergillus niger biomass, an industrial by-product of citric acid fermentation is an emergent source of glycoderivatives with applications in biofuel, cosmetics, feed, energy, food, medicine, and nanotechnology. In this study, the effect of purified neutral protease for deprotenization of fungal biomass studied at various levels (0, 5, 10, 20 and 40 U/100 mg of biomass) and the saccharification of fungal biomass was evaluated with amylolytic enzymes and chitosanases. The efficiency of deproteinization of fungal biomass was based on the enzyme concentration and contact time. Protease at a concentration of 20 U/100 mg of dry biomass and with a contact time of 8 h achieved 30% final deproteinization. No effect on saccharification of A. niger biomass was observed by treatment with purified amylolytic enzymes. Meanwhile, the endo- and exo-chitosanases treatment yielded 54 g of g reducing sugars (equivalent to amino sugars)/ kg of fungal biomass, which can be employed for tailor-made carbohydrate production.
{"title":"Environmental Friendly Deproteinization and Saccharification of Industrial Fungal Biomass by Enzymatic Processing","authors":"J. Fuentes, N. Balagurusamy, Carolina E. Vita, R. Hours, Cristóbal N. Aguilar, S. Cavalitto","doi":"10.17642/jcc.23.3.2","DOIUrl":"https://doi.org/10.17642/jcc.23.3.2","url":null,"abstract":"Aspergillus niger biomass, an industrial by-product of citric acid fermentation is an emergent source of glycoderivatives with applications in biofuel, cosmetics, feed, energy, food, medicine, and nanotechnology. In this study, the effect of purified neutral protease for deprotenization of fungal biomass studied at various levels (0, 5, 10, 20 and 40 U/100 mg of biomass) and the saccharification of fungal biomass was evaluated with amylolytic enzymes and chitosanases. The efficiency of deproteinization of fungal biomass was based on the enzyme concentration and contact time. Protease at a concentration of 20 U/100 mg of dry biomass and with a contact time of 8 h achieved 30% final deproteinization. No effect on saccharification of A. niger biomass was observed by treatment with purified amylolytic enzymes. Meanwhile, the endo- and exo-chitosanases treatment yielded 54 g of g reducing sugars (equivalent to amino sugars)/ kg of fungal biomass, which can be employed for tailor-made carbohydrate production.","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89616020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Removal of Phosphate Anions from Aqueous Solutions Using a Granular Adsorbent Made with Chitosan and Drinking Water Treatment Sludge","authors":"Eun-sik Kim, Soon-Do Yoon, W. Shim, Min-Jin Hwang","doi":"10.17642/jcc.23.3.4","DOIUrl":"https://doi.org/10.17642/jcc.23.3.4","url":null,"abstract":"","PeriodicalId":15363,"journal":{"name":"Journal of Chitin and Chitosan","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89872407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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