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Effect of Kelakai (Stenochlaena palutris) Extract on Organophosphate Pesticide Exposure: Cytotoxic Studies in Ovo and in Silico Kelakai(Stenochlaena palutris)提取物对有机磷农药暴露的影响:卵细胞和硅学细胞毒性研究
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.14583
Nafisah Nafisah, Sarmila Sarmila, Hanna Habibah, Indah Saputri, Indah Setiawati, N. Komari
Vegetable farmers in South Kalimantan use pesticides to protect crops from pests. The active ingredient of the pesticide has a broad toxic effect on target and non-target organisms. Continuous exposure to pesticides causes cancer. The plant of mantle (Stenochlaena palustris) is thought to have potential cytotoxic activity against cancer cell growth. The aim of this study was to examine the potential activity of chemical compounds in anaplastic lymphoma kinase (ALK) exposed to organophosphate pesticides in silico and in ovo. The in silico study used molecular docking and virtual screening methods. The methanol extract of malachite was obtained by maceration method. The in ovo study was carried out by injecting pesticide compounds and methanol extracts of methanol at doses of 0.5 and 1 ppm to free-range chicken eggs. The results of the in silico analysis showed that ethion and neophytadiene had the most negative G values, namely -8.62 kcal/mol and -8.39 kcal/mol, while the natural ligand 8 LY A 500 was -9.19 kcal/mol. The similarity of the type and number of residues in the binding complex between ethion and neophyadiene ligands with ALK protein indicates the potential for competition between ethion and neophyadiene when bound to ALK protein. Neofitadiene is suspected as a compound that has potential as anti-cancer by inhibiting the growth of ALK. Anchovy extract is considered to be able to slow down the rate of cell damage in chicken embryos caused by ethion with its inhibitory ability so that the cell surface is not damaged quickly.
南加里曼丹的菜农使用杀虫剂来保护作物免受虫害。杀虫剂的有效成分对目标生物和非目标生物具有广泛的毒性作用。持续接触杀虫剂会致癌。人们认为芒草(Stenochlaena palustris)对癌细胞的生长具有潜在的细胞毒性。本研究的目的是通过硅学研究和卵生研究,考察暴露于有机磷农药的无性淋巴瘤激酶(ALK)中的化合物的潜在活性。硅学研究采用了分子对接和虚拟筛选方法。孔雀石的甲醇提取物通过浸泡法获得。体内研究是通过向土鸡蛋中注入剂量为 0.5 和 1 ppm 的农药化合物和甲醇提取物来进行的。硅学分析结果表明,乙硫磷和新对二甲苯的负G值最大,分别为-8.62 kcal/mol和-8.39 kcal/mol,而天然配体8 LY A 500的负G值为-9.19 kcal/mol。乙硫磷和新戊二烯配体与 ALK 蛋白结合复合物中残基类型和数量的相似性表明,当与 ALK 蛋白结合时,乙硫磷和新戊二烯之间可能存在竞争。新戊二烯被怀疑是一种通过抑制 ALK 的生长而具有抗癌潜力的化合物。人们认为,凤尾鱼提取物具有抑制能力,能够减缓乙硫磷对鸡胚细胞的破坏速度,从而使细胞表面不会很快受到破坏。
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引用次数: 0
Evaluation of Antioxidant Activity in Lemon Juice (Citrus limon) Marketed in Makassar City Using the DPPH (2,2 diphenyl-1-picrylhydrazyl) Method 使用 DPPH(2,2-二苯基-1-苦基肼)法评估望加锡市销售的柠檬汁(柠檬)中的抗氧化活性
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.14903
A. S. Asmawati, Rahmi Rahmi, Desi Reski Fajar, Ira Widya Sari
Human lifestyle is currently experiencing many changes that lead to unhealthy lifestyles. An instant lifestyle can trigger degenerative diseases, which are initiated by excessive oxidation reactions in the body, which can cause the formation of free radicals. Lemon juice, derived from the Citrus limon plant, is considered a functional beverage due to its antioxidant properties, which have the potential to counteract the harmful effects of free radicals. This study aimed to measure the antioxidant activity of lemon juice beverage based on the IC50 value. The present study adopted a descriptive laboratory approach, utilizing lemon juice beverages from three distinct brands as representative samples. The antioxidant activity test was carried out by adding DPPH and measured using UV-Vis Spectrophotometry. The antioxidant levels obtained were 1,091.17 µg/mL for sample A, 1,114.50 µg/mL for sample B, and 527,261 µg/mL for sample C. All samples exhibited antioxidant activity values that fell within the "very weak" classification, as shown by IC50 values above 200 µg/mL.
人类的生活方式目前正经历着许多变化,这些变化导致了不健康的生活方式。急功近利的生活方式会诱发退行性疾病,而退行性疾病是由体内过度的氧化反应引起的,氧化反应会导致自由基的形成。柠檬汁源自柑橘类植物柠檬,因其抗氧化特性而被视为一种功能性饮料,具有抵消自由基有害影响的潜力。本研究旨在根据 IC50 值测量柠檬汁饮料的抗氧化活性。本研究采用描述性实验室方法,以三个不同品牌的柠檬汁饮料为代表样本。抗氧化活性测试通过添加 DPPH 进行,并使用紫外可见分光光度法进行测量。样品 A 的抗氧化剂含量为 1 091.17 微克/毫升,样品 B 为 1 114.50 微克/毫升,样品 C 为 527 261 微克/毫升。
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引用次数: 0
A Review: Optimum Conditions for Magnetite Synthesis (Fe3O4) 综述:磁铁矿合成(Fe3O4)的最佳条件
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.15134
Dominikus Niholan Tukan, L. Rosmainar, Kustomo Kustomo, Rasidah Rasidah
Magnetite, chemically represented as Fe3O4, is an opaque substance characterized by its black coloration. It possesses a significant saturation magnetization value, denoting a substantial magnetic strength compared to alternative materials. Magnetite (Fe3O4) has significant use across several domains, including its deployment as a catalyst and sensor in the medical sector, such as drug delivery, hyperthermia therapy, and magnetic resonance imaging (MRI). The synthesis of magnetite (Fe3O4) can be achieved using several methodologies, such as solvothermal, sol-gel, solid state, reverse micelle, microwave plasma synthesis, freeze drying, ultrasound irradiation, hydrothermal, and coprecipitation techniques. The co-preparation technique is extensively employed for magnetite synthesis due to its simplicity, ease of usage, and ability to generate magnetic particles at the nanoscale scale without elevated temperatures. The outcome of the coprecipitation process is influenced by various factors such as the nature of the salt, molar ratio, pH level, stirring rate, and reaction temperature. This article aims to examine the impact of many factors, including the salt type, molar ratio, pH level, stirring rate, and reaction temperature, on the production of Magnetite (Fe3O4) through coprecipitation. It was found that Magnetite (Fe3O4) synthesis can produce nanoparticle size material with great magnetic power, namely at pH 8, temperature 70°C, and stirring rate of 10,000 rpm by using FeCl3 and FeCl2 as precursors Fe3+ and Fe2+ with a ratio of 1.5:1 and using precipitating base NH4OH.
磁铁矿的化学名称为 Fe3O4,是一种不透明物质,其特点是呈黑色。它具有显著的饱和磁化值,表示与其他材料相比具有很强的磁性。磁铁矿(Fe3O4)在多个领域都有重要用途,包括在药物输送、热疗和磁共振成像(MRI)等医疗领域用作催化剂和传感器。磁铁矿(Fe3O4)的合成可采用多种方法,如溶热、溶胶-凝胶、固态、反胶束、微波等离子合成、冷冻干燥、超声辐照、水热和共沉淀技术。共沉淀技术因其简单、易用以及无需高温即可生成纳米级磁性颗粒的能力而被广泛用于磁铁矿合成。共沉淀过程的结果受多种因素的影响,如盐的性质、摩尔比、pH 值、搅拌速度和反应温度。本文旨在研究盐的种类、摩尔比、pH 值、搅拌速率和反应温度等多种因素对共沉淀法生产磁铁矿(Fe3O4)的影响。研究发现,以 FeCl3 和 FeCl2 为前驱体,Fe3+ 和 Fe2+ 的比例为 1.5:1,使用沉淀碱 NH4OH,在 pH 值为 8、温度为 70°C、搅拌速率为 10,000 rpm 的条件下,合成磁铁矿(Fe3O4)可制备出具有强大磁力的纳米颗粒材料。
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引用次数: 0
Synthesis of Nano Silver From Melastoma malabathricum Leaf Extracts Modified PVA and its Antibacterial Activity Test 从改性 PVA 的 Melastoma malabathricum 叶提取物中合成纳米银及其抗菌活性测试
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.13862
Della Puspita Indriyani, V. Fabiani, Fajar Indah Puspita Sari
Synthesis of silver nanoparticles from the extract of senduduk leaf (Melastoma malabathricum) with modified polyvinyl alcohol (PVA) has been carried out. The purpose of this study was to determine the effect of the addition of PVA on the characteristics of silver nanoparticles formed using the extract of sensaat leaf (Melastoma malabathricum). Variations in the concentration of PVA used were 0%; 0.75%; 1.5%; and 3%. Synthesis was carried out in a ratio of 1:10:1 (Extract: AgNO3: PVA) for 2 hours. The characteristics of the synthesis of nanoparticles using a UV-Vis spectrophotometer resulted in a consecutive wavelength of 414 nm; 435 nm; 438 nm; and 420 nm. To determine the particle size using Particle Size Analyzer (PSA) analysis at 0% PVA and 3% PVA with sizes obtained 61.9 nm and 216.2 nm. The average particle size using X-Ray Diffraction (XRD) analysis showed the results in the form of crystals and using the Debye-Scherrer equation to obtain the average particle size of PVA 0% 22.97 nm and PVA 3% 10.15 nm. Antibacterial activity test on silver nanoparticles used disc diffusion method, which showed that silver nanoparticles had moderate to strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria.Keywords: Bioreductor, senduduk leaf extract, silver nanoparticles, and polyvinyl alcohol
用改性聚乙烯醇(PVA)从森杜鹃叶(Melastoma malabathricum)提取物中合成了纳米银粒子。本研究的目的是确定添加 PVA 对使用森达叶(Melastoma malabathricum)提取物形成的银纳米粒子特性的影响。所使用的 PVA 浓度分别为 0%、0.75%、1.5% 和 3%。合成以 1:10:1 的比例(提取物:AgNO3:PVA)进行,时间为 2 小时。使用紫外可见分光光度计检测合成纳米粒子的特征,结果显示连续波长分别为 414 nm、435 nm、438 nm 和 420 nm。使用粒度分析仪(PSA)分析 0% PVA 和 3% PVA 的粒度,得到的粒度分别为 61.9 nm 和 216.2 nm。使用 X 射线衍射(XRD)分析平均粒度,结果显示其为晶体形式,并使用 Debye-Scherrer 公式得出 PVA 0% 的平均粒度为 22.97 nm,PVA 3% 的平均粒度为 10.15 nm。纳米银颗粒的抗菌活性测试采用了盘扩散法,结果表明纳米银颗粒对金黄色葡萄球菌和大肠杆菌具有中等到较强的抗菌活性:生物诱导剂 森古杜克叶提取物 纳米银颗粒和聚乙烯醇
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引用次数: 0
Utilization of Ferrate Ion (FeO42-) as Oxidizing Agent for Reducing Color Intensity of Peat Water 利用铁酸根离子(FeO42-)作为氧化剂降低泥炭水的色度
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.14832
Retno Agnestisia, K. Karelius, R. M. Iqbal, Dyah Ayu Pramoda Wardani, Midun Efendi Patar Sihombing, Sri Yulandari Simangunsong, Junita Dongoran
Peat water from Central Kalimantan is generally yellow to brownish-red in color. It causes peat water to not be used as a source of water for daily needs. Humic acid, fulvic acid, and humin are organic compounds that cause peat water to become colored. One alternative step that can be taken for reducing the color intensity of peat water is to degrade color-causing organic compounds by using an oxidizing agent. A hexavalent form of iron ion (Fe6+), commonly called ferrate (FeO42-) in potassium ferrate compound, is a powerful oxidizing agent used in the present study. Potassium ferrate (K2FeO4) was synthesized by a wet oxidation method and characterized using a UV-Vis spectrophotometer and an X-Ray Diffractometer (XRD). The ferrate solution is purplish-red in color with a maximum wavelength of 510 nm. The XRD pattern shows strong diffraction peaks at 2θ which are characteristic of K2FeO4. The ferrate solution was then used to degrade color-causing organic compounds  in the peat water from Central Kalimantan. Determination of the optimum degradation conditions was monitored by the absorbance decrease of organic compounds in peat water at a wavelength of 370 nm. The results showed that ferrate solution with a concentration of 80 ppm was able to degrade organic compounds in peat water at pH 8, with a  removal efficiency of 100%. Keywords: oxidizing agent, ferrate ion (FeO42-), peat water
中加里曼丹的泥炭水一般呈黄色至棕红色。这导致泥炭水不能用作满足日常需要的水源。腐殖酸、富维酸和腐殖质是导致泥炭水变色的有机化合物。降低泥炭水颜色强度的另一个方法是使用氧化剂降解导致颜色的有机化合物。铁酸钾化合物中六价形式的铁离子(Fe6+),通常称为铁酸(FeO42-),是本研究中使用的一种强效氧化剂。铁酸钾(K2FeO4)是通过湿氧化法合成的,并使用紫外-可见分光光度计和 X 射线衍射仪(XRD)对其进行了表征。铁酸溶液呈紫红色,最大波长为 510 纳米。X 射线衍射图在 2θ 处显示出强烈的衍射峰,这是 K2FeO4 的特征。随后,该铁酸盐溶液被用于降解中加里曼丹泥炭水中的致色有机化合物。最佳降解条件是通过泥炭水中有机化合物在波长为 370 纳米处的吸光度下降来监测的。结果表明,在 pH 值为 8 的条件下,浓度为 80 ppm 的铁酸溶液能够降解泥炭水中的有机化合物,去除率达到 100%。关键词:氧化剂、铁离子(FeO42-)、泥炭水
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引用次数: 0
Qualitative and Quantitative Characterization of Secondary Metabolites of Qust' Al-Hindi (Saussurea Lappa) Plants Qust' Al-Hindi(Saussurea Lappa)植物次生代谢物的定性和定量表征
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.14831
Nila Tanyela Berghuis, Ana Lailatul Farida, A. Ilmiawati
Utilization of plants that have the potential as herbal medicines, including the Qust' Al-Hindi (Saussurea Lappa) plant. Identification of the secondary metabolite content of the Qust' Al-Hindi Plant (Saussurea Lappa) through Phytochemical analysis in the form of flavonoid, alkaloid, steroid, terpenoid, tannin, and saponin tests on the extraction results as well as the calculation of flavonoid levels which are the largest content of this plant carried out using the UV method. -Vis. The characterization of the presence of a class of secondary metabolites contained in the plant was analyzed using FTIR-ATR spectroscopy. The results of this study indicate that the plant contains secondary metabolites in the form of flavonoid compounds, alkaloids, terpenoids, and tannins. The flavonoid content obtained was 223.33 ± 66.5 mgQE/g, while the results of the FTIR-ATR test showed that the extract contained the same functional groups as flavonoids, alkaloids, terpenoids, and tannins, namely the O-H, C-H, C=C functional groups. C=O and C-O contained in the structure of flavonoids, alkaloid compounds are known to have a distinctive functional group, namely N-H, for terpenoids to have a distinctive functional group, namely C-H, namely CH2 and CH3, there is also CH and there is a C-O group which is a typical functional group of compounds tannins.
利用具有草药潜力的植物,包括 Qust' Al-Hindi(Saussurea Lappa)植物。通过植物化学分析鉴定 Qust' Al-Hindi 植物(Saussurea Lappa)的次生代谢物含量,包括类黄酮、生物碱、甾体、萜类、单宁和皂苷,对提取结果进行测试,并使用紫外线法计算黄酮含量,黄酮是这种植物中含量最高的物质。-紫外线利用傅立叶变换红外-ATR 光谱分析了该植物所含的一类次生代谢物的特征。研究结果表明,该植物含有黄酮类化合物、生物碱、萜类化合物和单宁酸等形式的次生代谢物。获得的黄酮含量为 223.33 ± 66.5 mgQE/g,而傅立叶变换红外-ATR 测试结果表明,提取物含有与黄酮类化合物、生物碱、萜类化合物和单宁酸相同的官能团,即 O-H、C-H、C=C 官能团。众所周知,黄酮类化合物结构中含有的 C=O 和 C-O,生物碱类化合物有一个独特的官能团,即 N-H,萜类化合物有一个独特的官能团,即 C-H,即 CH2 和 CH3,还有 CH 和一个 C-O 官能团,这是单宁类化合物的典型官能团。
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引用次数: 0
Pretreatment of Aloe Vera and Its Effect on the Properties of Flour and Vermicelli Produced 芦荟预处理及其对面粉和粉丝性能的影响
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.14900
Nova Ellya Widiyati, Iffah Muflihati, Rini Umiyati, A. R. Affandi
Aloe vera powder has a less bright color due to the browning reaction. Pretreatment was carried out to improve the quality of aloe vera flour. Aloe vera flour can be used to manufacture food products, one of which is vermicelli. This study aimed to determine the effect of different pretreatments, namely blanching, sodium metabisulfite immersion, sodium bisulfite immersion, and steam blanching, on the characteristics of aloe vera flour and its application to vermicelli. The research steps included aloe vera pretreatment and aloe vera flour production, vermicelli production with aloe vera flour substitution, and analysis. The results showed that the pretreatment produced aloe vera and vermicelli flour with a brighter color. Pretreatment also affects water content, water-holding capacity, oil-holding capacity, and cooking properties. But the pretreatment did not affect the sensory properties of vermicelli.
由于褐变反应,芦荟粉的颜色不太鲜艳。进行预处理可提高芦荟粉的质量。芦荟粉可用来生产食品,粉丝就是其中之一。本研究旨在确定不同预处理方法(即焯水、焦亚硫酸钠浸泡、亚硫酸氢钠浸泡和蒸汽焯水)对芦荟粉特性及其在粉丝中应用的影响。研究步骤包括芦荟预处理和芦荟粉生产、芦荟粉替代芦荟粉生产粉丝以及分析。结果表明,预处理生产出的芦荟粉和粉丝颜色更鲜艳。预处理还会影响含水量、持水量、持油量和烹饪性能。但预处理并不影响粉丝的感官特性。
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引用次数: 0
Briquette from Candlenut Shell Charcoal and Polypropylene Plastic Waste From The Kefamenanu Landfill 用来自凯法梅纳努垃圾填埋场的烛台壳木炭和聚丙烯塑料废料制成煤球
Pub Date : 2023-10-23 DOI: 10.20527/jstk.v17i2.13738
Giovanny Robertho Wolfram Lake, G. D. Gelyaman, S. M. Kolo
This research aimed to determine the optimum composition of polypropylene plastic and candlenut shell charcoal in briquettes production. The percentage of briquette mixture was CK100:0PP, CK75:25PP, CK50:50PP, CK25:75PP and CK0:100PP. The moisture content of the briquettes obtained was as follows CK100:0PP (6.33%), CK75:25PP (4.18%), CK50:50PP (2.63%), CK25:75PP (1.90%) and CK0: 100PP (0.77%). Ash content was CK100:0PP (0.51%), CK75:25PP (18.82%), CK50:50PP (9.09%), CK25:75PP (3.26%) and CK0:100PP (1.74%). The level of flying substances was CK100:0PP (33.53%), CK75:25PP (51.52%), CK50:50PP (52.37%), CK25:75PP (60.99%) and CK0:100PP (72.96%). Fixed carbon was CK100:0PP (59.63%), CK75:25PP (25.48%), CK50:50PP (35.91%), CK25:75PP (33.85%) and CK0:100PP (24.52 %). Calorific value was CK100:0PP (2,902.31 cal/gr), CK75:25PP (9,292.45 cal/gr), CK50:50PP (8,155.53 cal/gr), CK25:75PP (9,094.69 cal/gr) and CK0:100PP (10,808.30 cal/gr). Compressive strength was CK75:25PP (50.31 Kgf/cm2), CK50:50PP (44.67 Kgf/cm2), CK25:75PP (36.11 Kgf/cm2) and CK0:100PP (33.85 Kgf/cm2). The optimum composition of briquettes from a mixture of polypropylene plastic and candlenut shell charcoal was CK25:75PP.
这项研究旨在确定聚丙烯塑料和烛壳炭在煤球生产中的最佳成分。压块混合物的比例分别为 CK100:0PP、CK75:25PP、CK50:50PP、CK25:75PP 和 CK0:100PP。压块的水分含量分别为 CK100:0PP (6.33%)、CK75:25PP (4.18%)、CK50:50PP (2.63%)、CK25:75PP (1.90%) 和 CK0: 100PP (0.77%)。灰分含量为 CK100:0PP (0.51%)、CK75:25PP (18.82%)、CK50:50PP (9.09%)、CK25:75PP (3.26%) 和 CK0:100PP (1.74%)。飞行物质含量分别为 CK100:0PP (33.53%)、CK75:25PP (51.52%)、CK50:50PP (52.37%)、CK25:75PP (60.99%) 和 CK0:100PP (72.96%)。固定碳为 CK100:0PP (59.63%)、CK75:25PP (25.48%)、CK50:50PP (35.91%)、CK25:75PP (33.85%) 和 CK0:100PP (24.52%)。热值分别为:CK100:0PP(2,902.31 卡/克)、CK75:25PP(9,292.45 卡/克)、CK50:50PP(8,155.53 卡/克)、CK25:75PP(9,094.69 卡/克)和 CK0:100PP(10,808.30 卡/克)。抗压强度分别为 CK75:25PP(50.31 千克力/平方厘米)、CK50:50PP(44.67 千克力/平方厘米)、CK25:75PP(36.11 千克力/平方厘米)和 CK0:100PP(33.85 千克力/平方厘米)。聚丙烯塑料和烛台壳木炭混合物压块的最佳成分是 CK25:75PP。
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引用次数: 0
Macro and Micro Mineral Composition of Haruan Fish (Channa striata) in Banjar District, South Kalimantan 南加里曼丹班贾尔地区Haruan鱼(Channa striata)宏观和微观矿物组成
Pub Date : 2023-02-14 DOI: 10.20527/jstk.v17i1.12563
R. Yunus, D. Ariyani, Muhammad Aisy
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引用次数: 0
Antioxidant and Antiproliferative Activities of Methanol Extract from Melaleuca cajuputi subsp. Cumingiana [Turcz.] Fruit 千层木甲醇提取物的抗氧化和抗增殖活性。Cumingiana [Turcz。]水果
Pub Date : 2023-02-14 DOI: 10.20527/jstk.v17i1.13055
Isnaini Isnaini, Rosinta Dewi Achmadiyah, Gelvia Awaeh, H. Khatimah, A. Yasmina
Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow (M. cajuputi) is a plant that is easily found in Banjarmasin. M. cajuputi contains phytochemical compounds in the form of polyphenols including flavonoids, quinons, saponins, and alkaloids that are thought to have antioxidant and antiproliferation activities. The aim of this research was to find out analyze antioxidant and antiproliferation activity of M. cajuputi fruit methanol extract. The antioxidant activity was tested using the DPPH method. The activities were observed in IC50 and were measured using the UV-VIS spectrophotometer at a wavelength of 517 nm. To test the antiproliferation, true experimental with post-test was applied in this research.  Animal used in this study were 30 mature zebras (length > 2.5 cm) which were grouped into 4, namely the negative control group (DMSO 0.05%), the methanol extract group of M. cajuputi with concentrations of 18.5 ppm, 37 ppm and 74 ppm. The bound variable in this study was antiproliferation activity in the tail of an amputated fish. Data analysis was measured by one-way ANOVA and Post-Hoc Tukey HSD tests. Phytochemical results obtained the presence of phenol compounds, cuinons, flavonoids, alkaloids, saponins, tannins, steroids and terpenoids. Methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit was at IC50 of 15.50 ppm (95% CI 8.31- 32.72).  The antiproliferation activity of zebrafish tails increased in the administration of fruit extract Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow in consentration of 74 ppm (p<0.05), both on day 4 and day 8 of measurement when compared to negative controls. It can be concluded that methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit has antiproliferative activity against the growth of amputated zebrafish tails.
千层木。Cumingiana [Turcz。巴洛(M. cajuputi)是一种在Banjarmasin很容易找到的植物。枸杞含有多酚类植物化合物,包括类黄酮、醌、皂苷和生物碱,被认为具有抗氧化和抗增殖活性。本研究旨在分析枣果甲醇提取物的抗氧化和抗增殖活性。采用DPPH法测定其抗氧化活性。用紫外-可见分光光度计在517 nm波长处测定其活性。为验证其抗增殖作用,本研究采用真实后验实验。实验动物为30头体长> 2.5 cm的成熟斑马,分为4组,分别为阴性对照组(DMSO 0.05%)和浓度分别为18.5 ppm、37 ppm和74 ppm的马尾草甲醇提取物组。本研究的结合变量是截断鱼尾部的抗增殖活性。数据分析采用单因素方差分析和事后Tukey HSD检验。植物化学结果显示酚类化合物、醌类化合物、黄酮类化合物、生物碱、皂苷、单宁、类固醇和萜类化合物的存在。木香千层甲醇提取物[j]。巴罗果的IC50为15.50 ppm (95% CI 8.31 ~ 32.72)。千层果提取物对斑马鱼尾的抗增殖活性有增强作用。Cumingiana [Turcz。] Barlow浓度为74 ppm (p<0.05),在测量的第4天和第8天与阴性对照相比。综上所述,千层木甲醇提取物具有较好的抗氧化活性。巴罗果对斑马鱼断尾有抗增殖作用。
{"title":"Antioxidant and Antiproliferative Activities of Methanol Extract from Melaleuca cajuputi subsp. Cumingiana [Turcz.] Fruit","authors":"Isnaini Isnaini, Rosinta Dewi Achmadiyah, Gelvia Awaeh, H. Khatimah, A. Yasmina","doi":"10.20527/jstk.v17i1.13055","DOIUrl":"https://doi.org/10.20527/jstk.v17i1.13055","url":null,"abstract":"Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow (M. cajuputi) is a plant that is easily found in Banjarmasin. M. cajuputi contains phytochemical compounds in the form of polyphenols including flavonoids, quinons, saponins, and alkaloids that are thought to have antioxidant and antiproliferation activities. The aim of this research was to find out analyze antioxidant and antiproliferation activity of M. cajuputi fruit methanol extract. The antioxidant activity was tested using the DPPH method. The activities were observed in IC50 and were measured using the UV-VIS spectrophotometer at a wavelength of 517 nm. To test the antiproliferation, true experimental with post-test was applied in this research.  Animal used in this study were 30 mature zebras (length > 2.5 cm) which were grouped into 4, namely the negative control group (DMSO 0.05%), the methanol extract group of M. cajuputi with concentrations of 18.5 ppm, 37 ppm and 74 ppm. The bound variable in this study was antiproliferation activity in the tail of an amputated fish. Data analysis was measured by one-way ANOVA and Post-Hoc Tukey HSD tests. Phytochemical results obtained the presence of phenol compounds, cuinons, flavonoids, alkaloids, saponins, tannins, steroids and terpenoids. Methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit was at IC50 of 15.50 ppm (95% CI 8.31- 32.72).  The antiproliferation activity of zebrafish tails increased in the administration of fruit extract Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow in consentration of 74 ppm (p<0.05), both on day 4 and day 8 of measurement when compared to negative controls. It can be concluded that methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit has antiproliferative activity against the growth of amputated zebrafish tails.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"154 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131832827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Jurnal Berkala Ilmiah Sains dan Terapan Kimia
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