Carbon-free electrochemical synthesis of ammonia is a promising technology for CO2 emission reduction. This study aims to explore the electrocatalytic activity of A-site Ba-doped perovskite cathode catalyst (La0.6Ba0.4Fe0.8Cu0.2O3-δ, LBFCu) for ammonia synthesis from water and nitrogen. LBFCu was prepared via the sol-gel method using combined EDTA-citrate complexing agents and characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM). Ammonia was successfully synthesised from water and nitrogen under atmospheric pressure, and LBFCu mixed with Ce0.8Gd0.18Ca0.02O2-δ (CGDC) was used as a cathode. When a voltage was applied to the cell containing CGDC-carbonate composite solid electrolyte, ammonia formation was observed at 375, 400, 425 and 450 °C. At 400 °C and 1.4 V, the maximum rate of ammonia production was achieved at 4.0×10-11 mol s-1 cm-2, which corresponds to Faradaic efficiency of ~ 0.06 % at the current density of 19 mA cm-2. According to the findings, the synthesis of ammonia directly from water and nitrogen may be considered a promising green synthesis technology.
{"title":"Synthesis of ammonia from water and nitrogen using a compo-site cathode based on La0.6Ba0.4Fe0.8Cu0.2O3-δ-Ce0.8Gd0.18Ca0.02O2-δ","authors":"Ibrahim Ali Ahmed Amar","doi":"10.5599/jese.1535","DOIUrl":"https://doi.org/10.5599/jese.1535","url":null,"abstract":"Carbon-free electrochemical synthesis of ammonia is a promising technology for CO2 emission reduction. This study aims to explore the electrocatalytic activity of A-site Ba-doped perovskite cathode catalyst (La0.6Ba0.4Fe0.8Cu0.2O3-δ, LBFCu) for ammonia synthesis from water and nitrogen. LBFCu was prepared via the sol-gel method using combined EDTA-citrate complexing agents and characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM). Ammonia was successfully synthesised from water and nitrogen under atmospheric pressure, and LBFCu mixed with Ce0.8Gd0.18Ca0.02O2-δ (CGDC) was used as a cathode. When a voltage was applied to the cell containing CGDC-carbonate composite solid electrolyte, ammonia formation was observed at 375, 400, 425 and 450 °C. At 400 °C and 1.4 V, the maximum rate of ammonia production was achieved at 4.0×10-11 mol s-1 cm-2, which corresponds to Faradaic efficiency of ~ 0.06 % at the current density of 19 mA cm-2. According to the findings, the synthesis of ammonia directly from water and nitrogen may be considered a promising green synthesis technology.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"370 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74201627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Z. Mohammadi, Y. Baghelani, Farideh Mousazadeh, Shamsi Rahimi, Maryam Mohammad-Hassani
In this paper, a new electrochemical sensor was reported for the determination of amaranth in drink soft. In this sensor, activated carbon-Co3O4 nanocomposite (AC-Co3O4) was employed as electrode modifying materials. The introduction of high pores to the activated carbon not only can in favor of more amaranth molecules adsorbed to the surface of the working electrode through the pores, but also may benefit fast electron diffusion in the electrochemical detection process. So, the AC-Co3O4 modified electrode enhanced its electrochemical signal obviously in the determination of amaranth in drink soft and exhibited a wider linear response ranging from 0.1 to 215.0 µM with a low detection limit of 10.0 nM (based on 3Sb/m). This work offers a new route in developing new electrochemical sensors for the determination of colorants additives and other hazard components in drink soft.
{"title":"Electrochemical determination of amaranth in food samples using activated carbon-Co3O4 modified electrode","authors":"S. Z. Mohammadi, Y. Baghelani, Farideh Mousazadeh, Shamsi Rahimi, Maryam Mohammad-Hassani","doi":"10.5599/jese.1531","DOIUrl":"https://doi.org/10.5599/jese.1531","url":null,"abstract":"In this paper, a new electrochemical sensor was reported for the determination of amaranth in drink soft. In this sensor, activated carbon-Co3O4 nanocomposite (AC-Co3O4) was employed as electrode modifying materials. The introduction of high pores to the activated carbon not only can in favor of more amaranth molecules adsorbed to the surface of the working electrode through the pores, but also may benefit fast electron diffusion in the electrochemical detection process. So, the AC-Co3O4 modified electrode enhanced its electrochemical signal obviously in the determination of amaranth in drink soft and exhibited a wider linear response ranging from 0.1 to 215.0 µM with a low detection limit of 10.0 nM (based on 3Sb/m). This work offers a new route in developing new electrochemical sensors for the determination of colorants additives and other hazard components in drink soft.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"51 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78798406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A huge amount of oily wastewater is discharged annually from several industries like petroleum and petrochemical factories. Scientists and researchers are permanently concentrated on creating conventional technologies or identifying novel treatment options for oily wastewaters, since they need to be treated before being discharged into the soil and aquatic ecosystems. Electrocoagulation technology (ECT) is an electrochemical method employed to remove numerous pollutants from domestic and industrial wastewaters. This paper aims to review the recently published articles from 2018 to 2022 concerned with ECT for oily wastewater remediation. Based on the present review, it is obvious that ECT is strongly dependent on the value of electric current or voltage applied to provide the required amounts of electro-coagulants for efficient remediation, reaction time duration for the generation of electro-coagulants and pollutants elimination, and electrode configuration such as shape, type of metal, and distance between electrodes. Other operating parameters include solution pH (since some pollutants are removed based on their cationic or anionic nature), type of electrolyte which affects the electric conductivity and ohmic drop and stirring speed that may influence the contact among numerous ions throughout the EC reactor. The core findings show that the ECT is highly effective, eco-friendly, and cost-effective in eliminating organic and inorganic pollutants from oily wastewater.
{"title":"Treatment of oily wastewater by electrocoagulation technology: A general review (2018-2022)","authors":"M. Jasim, Forat Yasir Al Jaberi","doi":"10.5599/jese.1472","DOIUrl":"https://doi.org/10.5599/jese.1472","url":null,"abstract":"A huge amount of oily wastewater is discharged annually from several industries like petroleum and petrochemical factories. Scientists and researchers are permanently concentrated on creating conventional technologies or identifying novel treatment options for oily wastewaters, since they need to be treated before being discharged into the soil and aquatic ecosystems. Electrocoagulation technology (ECT) is an electrochemical method employed to remove numerous pollutants from domestic and industrial wastewaters. This paper aims to review the recently published articles from 2018 to 2022 concerned with ECT for oily wastewater remediation. Based on the present review, it is obvious that ECT is strongly dependent on the value of electric current or voltage applied to provide the required amounts of electro-coagulants for efficient remediation, reaction time duration for the generation of electro-coagulants and pollutants elimination, and electrode configuration such as shape, type of metal, and distance between electrodes. Other operating parameters include solution pH (since some pollutants are removed based on their cationic or anionic nature), type of electrolyte which affects the electric conductivity and ohmic drop and stirring speed that may influence the contact among numerous ions throughout the EC reactor. The core findings show that the ECT is highly effective, eco-friendly, and cost-effective in eliminating organic and inorganic pollutants from oily wastewater.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80034110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present day and age, engineered materials are making significant strides in their application in the biomedical sector. Human implants have piqued the interest of material and metallurgy researchers due to their unique properties. Machining the materials into implants customized for individual patients has been the standard practice nowadays. To contribute further to the same area, the current research work aims to investigate the machinability of T-L107.12 (a titanium-based human implant) using a non-traditional method called wire electrical discharge machining (WEDM). The machinability of the machining process has been evaluated based on the material removal rate using a roughness meter and the atomic force microscope. The further impact of the machining parameters on the output responses was analyzed based on the statistical analysis.
{"title":"Machinability behavior of human implant materials","authors":"Gurbhej Singh, Ranjit J. Singh, Jameel Gul","doi":"10.5599/jese.1514","DOIUrl":"https://doi.org/10.5599/jese.1514","url":null,"abstract":"In the present day and age, engineered materials are making significant strides in their application in the biomedical sector. Human implants have piqued the interest of material and metallurgy researchers due to their unique properties. Machining the materials into implants customized for individual patients has been the standard practice nowadays. To contribute further to the same area, the current research work aims to investigate the machinability of T-L107.12 (a titanium-based human implant) using a non-traditional method called wire electrical discharge machining (WEDM). The machinability of the machining process has been evaluated based on the material removal rate using a roughness meter and the atomic force microscope. The further impact of the machining parameters on the output responses was analyzed based on the statistical analysis.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"119 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85313948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Farideh Mousazadeh, S. Z. Mohammadi, Maryam Mohammadhasani-Pour
The current work aimed to fabricate a screen-printed graphite electrode (SPGE) modified by MnO2 nanorods (MnO2 NRs) for sensing hydrazine. Thus, a facile protocol was adopted to construct the MnO2 nanorods that were subsequently applied to modify the SPGE surface directly. As-synthesized MnO2 NRs/SPGE sensor exhibited a strong sensing behavior towards the hydrazine, with a large peak current and small oxidation potential. This electrochemical sensor in the optimized conditions to detect the hydrazine possessed a low detection limit (0.02 μM), a broad linear dynamic range (0.05–275.0 μM) and an admirable sensitivity (0.0625 μA μM-1). The sensor applicability was practically estimated in real water samples, which revealed successful recovery values.
{"title":"Novel electrochemical sensing platform for detection of hydrazine based on modified screen-printed graphite electrode","authors":"Farideh Mousazadeh, S. Z. Mohammadi, Maryam Mohammadhasani-Pour","doi":"10.5599/jese.1359","DOIUrl":"https://doi.org/10.5599/jese.1359","url":null,"abstract":"The current work aimed to fabricate a screen-printed graphite electrode (SPGE) modified by MnO2 nanorods (MnO2 NRs) for sensing hydrazine. Thus, a facile protocol was adopted to construct the MnO2 nanorods that were subsequently applied to modify the SPGE surface directly. As-synthesized MnO2 NRs/SPGE sensor exhibited a strong sensing behavior towards the hydrazine, with a large peak current and small oxidation potential. This electrochemical sensor in the optimized conditions to detect the hydrazine possessed a low detection limit (0.02 μM), a broad linear dynamic range (0.05–275.0 μM) and an admirable sensitivity (0.0625 μA μM-1). The sensor applicability was practically estimated in real water samples, which revealed successful recovery values.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"1 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88841005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current study focuses on the development of duplex Ni-P/Ni-Cu-P coatings by the electroless deposition method. Coatings are developed on mild steel substrates with Ni-Cu-P as the outer layer and Ni-P as the inner layer and vice versa. The coated samples are heat-treated at temperatures ranging between 200 to 800 °C during 1 and 4 h. Coated samples are characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). The effect of heat treatment temperature and its time duration on the hardness, friction and wear behaviour of both coatings are evaluated and compared. This would help in understanding how heat treatment influences the duplex system of coatings and helps in identifying the suitable condition of heat treatment for optimal performance of the coating. It is observed that heat treatment has a positive influence over the coating performance, which is the best when treated under optimal temperature and time duration conditions. The corrosion behaviour of the coatings is also assessed with the help of electrochemical techniques, viz. potentiodynamic polarization and electrochemical impedance spectroscopy. The results show that the duplex coatings can provide substantial protection to the mild steel substrates. Heat treatment is also found to have a significant influence on the corrosion behaviour of duplex coatings.
{"title":"Duplex electroless Ni-P/Ni-Cu-P coatings: Preparation, evaluation of microhardness, friction, wear, and corrosion performance","authors":"P. Biswas, S. Das, P. Sahoo","doi":"10.5599/jese.1392","DOIUrl":"https://doi.org/10.5599/jese.1392","url":null,"abstract":"The current study focuses on the development of duplex Ni-P/Ni-Cu-P coatings by the electroless deposition method. Coatings are developed on mild steel substrates with Ni-Cu-P as the outer layer and Ni-P as the inner layer and vice versa. The coated samples are heat-treated at temperatures ranging between 200 to 800 °C during 1 and 4 h. Coated samples are characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). The effect of heat treatment temperature and its time duration on the hardness, friction and wear behaviour of both coatings are evaluated and compared. This would help in understanding how heat treatment influences the duplex system of coatings and helps in identifying the suitable condition of heat treatment for optimal performance of the coating. It is observed that heat treatment has a positive influence over the coating performance, which is the best when treated under optimal temperature and time duration conditions. The corrosion behaviour of the coatings is also assessed with the help of electrochemical techniques, viz. potentiodynamic polarization and electrochemical impedance spectroscopy. The results show that the duplex coatings can provide substantial protection to the mild steel substrates. Heat treatment is also found to have a significant influence on the corrosion behaviour of duplex coatings.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"2 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90429543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The rapidly growing discipline of artificial intelligence (AI) seeks to develop software and computers that can do tasks that have historically required the intelligence of people. Machine learning (ML) is a subfield of AI that makes use of algorithms to "learn" from data's innate statistical patterns and structures to extrapolate information that is otherwise hidden. A growing emphasis on cosmetic dentistry has coincided with ZrO2‘s rise to prominence as a result of its improved biocompatibility, visually pleasant look, strong oxidation resistance, better mechanical properties, and lack of documented allergic responses. Advances in the field of AI and ML have led to novel applications of ZrO2 in dental devices for biological objectives. Artificial intelligence (AI) technologies have attracted a lot of attention in ZrO2-related research and therapeutic applications due to their ability to analyze data and discover connections between seemingly unrelated events. Specifically, their incorporation into zirconia is largely responsible for this. Zirconia's versatility in the scientific community means that how AI is used in the area varies with the specific directions in which zirconia is utilized. Therefore, this article primarily focuses on the use of AI in the biomedical use of ZrO2 in dentistry.
{"title":"Artificial intelligence in use of ZrO2 material in biomedical science","authors":"Jashanpreet Singh, Simranjith Singh, Amit Verma","doi":"10.5599/jese.1498","DOIUrl":"https://doi.org/10.5599/jese.1498","url":null,"abstract":"The rapidly growing discipline of artificial intelligence (AI) seeks to develop software and computers that can do tasks that have historically required the intelligence of people. Machine learning (ML) is a subfield of AI that makes use of algorithms to \"learn\" from data's innate statistical patterns and structures to extrapolate information that is otherwise hidden. A growing emphasis on cosmetic dentistry has coincided with ZrO2‘s rise to prominence as a result of its improved biocompatibility, visually pleasant look, strong oxidation resistance, better mechanical properties, and lack of documented allergic responses. Advances in the field of AI and ML have led to novel applications of ZrO2 in dental devices for biological objectives. Artificial intelligence (AI) technologies have attracted a lot of attention in ZrO2-related research and therapeutic applications due to their ability to analyze data and discover connections between seemingly unrelated events. Specifically, their incorporation into zirconia is largely responsible for this. Zirconia's versatility in the scientific community means that how AI is used in the area varies with the specific directions in which zirconia is utilized. Therefore, this article primarily focuses on the use of AI in the biomedical use of ZrO2 in dentistry.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"13 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84561620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ofuyekpone, A. A. Adediran, O. Utu, B. Onyekpe, U. Unueroh
Weight loss, polarization, and open circuit potential methods were used to investigate the corrosion inhibitory impact of Centrosema pubescens leaf extract on 304L austenitic stainless steel UNS S30403 in 1 M hydrochloric acid. This non-toxic extract behaves as a mixed-type inhibitor according to the polarization curves, thermodynamics and activation parameters. Both the weight loss calculations and potentiodynamic polarization investigations showed that 1.2 g L-1 was the optimal concentration of the leaf extract. While the weight loss method gave inhibition efficiency of 86.84 and 75.00 % after 10 and 60 days of immersion at the optimum concentration, polarization studies revealed inhibition efficiencies of 93.08 and 98.66 % at 303 and 333 K, respectively. The extract molecules adhered to the UNS S30403 surface according to Langmuir adsorption isotherm. The presence of the protective film on the UNS S30403 surface was confirmed by SEM, EDX, and XRD measurements. The inhibition performance of the leaf extract was noted to be a function of the extract concentration, immersion time and temperature. The FTIR analysis indicated an interaction between austenitic stainless steel UNS S30403 and the molecules of Centrosema pubescens leaf extract.
{"title":"Non-toxic leguminous plant leaf extract as an effective corrosion inhibitor of UNS S30403 in 1 M HCl","authors":"O. Ofuyekpone, A. A. Adediran, O. Utu, B. Onyekpe, U. Unueroh","doi":"10.5599/jese.1343","DOIUrl":"https://doi.org/10.5599/jese.1343","url":null,"abstract":"Weight loss, polarization, and open circuit potential methods were used to investigate the corrosion inhibitory impact of Centrosema pubescens leaf extract on 304L austenitic stainless steel UNS S30403 in 1 M hydrochloric acid. This non-toxic extract behaves as a mixed-type inhibitor according to the polarization curves, thermodynamics and activation parameters. Both the weight loss calculations and potentiodynamic polarization investigations showed that 1.2 g L-1 was the optimal concentration of the leaf extract. While the weight loss method gave inhibition efficiency of 86.84 and 75.00 % after 10 and 60 days of immersion at the optimum concentration, polarization studies revealed inhibition efficiencies of 93.08 and 98.66 % at 303 and 333 K, respectively. The extract molecules adhered to the UNS S30403 surface according to Langmuir adsorption isotherm. The presence of the protective film on the UNS S30403 surface was confirmed by SEM, EDX, and XRD measurements. The inhibition performance of the leaf extract was noted to be a function of the extract concentration, immersion time and temperature. The FTIR analysis indicated an interaction between austenitic stainless steel UNS S30403 and the molecules of Centrosema pubescens leaf extract.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"17 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88449035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This Special Issue entitled “Surface coatings: latest developments in the protection of steels from corrosion and erosion” focuses on significant advancements and developments in coating technologies in the protection of steels from corrosion and erosion in different operative environments. This Special Issue intends to cover original research and critical review articles on recent advances in various techniques to combat corrosion and erosion of steels.
{"title":"Latest developments in the protection of steels from corrosion and erosion","authors":"K. Goyal","doi":"10.5599/jese.1541","DOIUrl":"https://doi.org/10.5599/jese.1541","url":null,"abstract":"This Special Issue entitled “Surface coatings: latest developments in the protection of steels from corrosion and erosion” focuses on significant advancements and developments in coating technologies in the protection of steels from corrosion and erosion in different operative environments. This Special Issue intends to cover original research and critical review articles on recent advances in various techniques to combat corrosion and erosion of steels.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"78 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75726756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salma Nur Zakiyyah, D. Eddy, M. Firdaus, T. Subroto, Y. Hartati
A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein concentration. Box-Behnken’s design was employed to determine the optimal conditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltammetry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.
合成了一种天然二氧化硅和二氧化铈的纳米复合材料，对丝网印刷碳电极(SPCE)进行修饰，以开发一种检测尿液中上皮钠通道(ENaC)蛋白作为高血压生物标志物的适体传感器。方法步骤为水热法合成天然二氧化硅-二氧化铈纳米复合材料，从碱性硅砂中提取天然二氧化硅纳米颗粒，从硝酸铈中提取二氧化硅纳米颗粒，对SPCE/天然二氧化硅-二氧化铈进行改性，通过链亲和素-生物素固定化适体，检测ENaC蛋白浓度。采用Box-Behnken设计确定适体浓度(0.5 μg mL-1)、链霉亲和素孵育时间(30 min)和适体孵育时间(1 h)的最佳条件。差分脉冲伏安法(DPV)表征表明，[Fe(CN)6]3-/4-氧化还原峰电流从3.190 μA增加到9.073 μA，检测限和定量限分别为0.113和0.343 ng mL-1。该方法是一种简便、快速的尿液中ENaC水平监测方法。
{"title":"Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application","authors":"Salma Nur Zakiyyah, D. Eddy, M. Firdaus, T. Subroto, Y. Hartati","doi":"10.5599/jese.1455","DOIUrl":"https://doi.org/10.5599/jese.1455","url":null,"abstract":"A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein concentration. Box-Behnken’s design was employed to determine the optimal conditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltammetry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.","PeriodicalId":15660,"journal":{"name":"Journal of Electrochemical Science and Engineering","volume":"123 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78320097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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