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Evaluation of Leaves, Flowers, and Seeds of Coriander (Coriandrum sativum L.) through Microwave Drying and Ultrasonic-Assisted Extraction, for Biologically Active Components 通过微波干燥和超声波辅助萃取评估芫荽(Coriandrum sativum L.)叶、花和种子中的生物活性成分
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-16 DOI: 10.1155/2024/2378604
Ashiq Hussain, Muhammad Rehan Arif, Adnan Ahmed, Iqra Fiaz, Nabeela Zulfiqar, Muhammad Qasim Ali, Nida Firdous, Haya Fatima, Anjum Shehzad, Abdeen Elsiddig Elkhedir

The coriander plant (Coriandrum sativum L.) is well known for its antibacterial and antioxidant properties since it contains a considerable number of bioactive compounds. This property encourages the use of coriander in food because it has many health benefits and preserves food longer. The current study’s objective was to demonstrate the extraction of coriander’s three fractions (leaves, flowers, and seeds) using microwave drying and ultrasonic assistance, in order to identify its distinct functional components. After microwave drying, the highest amounts of ash, fat, fiber, and protein with values 6.39 ± 0.04, 10.10 ± 0.05, 10.14 ± 0.06, and 13.10 ± 0.03%, respectively, were observed in coriander seeds. Among macro- and microminerals analyzed, contents of Ca and Mg were found highest in coriander leaves, with values 689 ± 0.14 and 412 ± 0.04 mg/100 g, respectively, whereas Fe, Zn, and Mn were found highest in seeds with values 15.46 ± 0.02, 3.92 ± 0.02, and 1.29 ± 0.02 mg/100 g. Ultrasonic-assisted ethanolic extracts of microwave-dried coriander leaves presented significantly high (p < 0.05) total phenolic contents (253.45 ± 0.12 mg gallic acid equivalent/100 g), total flavonoid contents (98.15 ± 0.09 mg quercetin equivalent/100 g), and total antioxidant activity (47.32 ± 0.04 mg trolox/100 g), followed by seeds, while flowers presented lowest values. Significantly high (p < 0.05) antimicrobial activities were exhibited from extracts of coriander seeds, followed by leaves. It was concluded that leaves, flowers, and seeds of coriander all were rich source of nutritional components and bioactives, and microwave drying and ultrasonic-assisted extraction were proved useful techniques for maximum retention of these contents in powders and ethanolic extracts, respectively.

芫荽植物(Coriandrum sativum L.)因其抗菌和抗氧化特性而闻名,因为它含有大量生物活性化合物。由于芫荽具有许多健康益处,并能更长久地保存食物,因此这种特性促使人们在食品中使用芫荽。本研究的目的是展示利用微波干燥和超声波辅助提取芫荽的三种部分(叶、花和种子),以确定其独特的功能成分。微波干燥后,芫荽籽的灰分、脂肪、纤维和蛋白质含量最高,分别为 6.39±0.04%、10.10±0.05%、10.14±0.06% 和 13.10±0.03%。在分析的宏量和微量矿物质中,芫荽叶中钙和镁的含量最高,分别为 689±0.14 和 412±0.04 mg/100 g,而种子中铁、锌和锰的含量最高,分别为 15.46±0.02、3.92±0.02 和 1.29±0.02 mg/100 g。微波干燥芫荽叶片的超声波辅助乙醇提取物的总酚含量(253.45±0.12 毫克没食子酸当量/100 克)、总黄酮含量(98.15±0.09 毫克槲皮素当量/100 克)和总抗氧化活性(47.32±0.04 毫克三环氧化酶/100 克)显著较高(p<0.05),种子次之,而花的含量最低。芫荽种子提取物的抗菌活性明显较高(p<0.05),其次是叶子。结论是,芫荽的叶、花和种子都含有丰富的营养成分和生物活性物质,微波干燥和超声波辅助萃取被证明是分别在粉末和乙醇提取物中最大限度保留这些成分的有用技术。
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引用次数: 0
An In-Depth Overview of the Structural Properties, Health Benefits, and Applications of Resistant Dextrin 抗性糊精的结构特性、健康益处和应用概览
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-16 DOI: 10.1155/2024/8055063
Xiuli Wu, Jianwen Zhang, Xiangxuan Yan, Xuexu Wu, Qing Zhang, Mingran Luan

With the escalating prevalence of diabetes and obesity, resistant dextrin, renowned for its prebiotic properties and blood glucose-lowering physiological activity, has garnered significant attention. Resistant dextrin, a low-calorie, indigestible water-soluble dietary fiber processed from starch, has high solubility, low molecular weight, and good thermal stability. The established method for its preparation involves a combination of acid heat treatment and enzymatic purification. Within the human body, resistant dextrin confers numerous health benefits. It promotes a balanced intestinal microbiome, regulates blood glucose and lipid metabolism, and enhances satiety. Additionally, it exerts positive influences on the intestinal environment, aids in weight management, and alleviates chronic conditions, particularly diabetes. In the food industry, resistant dextrin is widely employed as a functional food additive to enhance the nutritional value and health benefits of various food products. However, there is a need for greater clarity regarding the structural characteristics of resistant dextrin and the potential interplay between its structure and physiological activity. This paper comprehensively reviews the preparation methods, structural properties, health benefits, and application areas of resistant dextrin. Additionally, it anticipates future trends in its development. The primary objective of this review is to offer theoretical guidance and fresh perspectives for further research, the innovation of functional products, and the expanded utilization of resistant dextrin.

随着糖尿病和肥胖症发病率的上升,抗性糊精因其益生元特性和降低血糖的生理活性而备受关注。抗性糊精是一种由淀粉加工而成的低热量、难消化的水溶性膳食纤维,具有高溶解性、低分子量和良好的热稳定性。其成熟的制备方法包括酸性热处理和酶解纯化。在人体内,抗性糊精对健康有诸多益处。它能促进肠道微生物群的平衡,调节血糖和血脂代谢,增强饱腹感。此外,它还对肠道环境产生积极影响,有助于控制体重,缓解慢性疾病,尤其是糖尿病。在食品工业中,抗性糊精被广泛用作功能性食品添加剂,以提高各种食品的营养价值和保健功效。然而,抗性糊精的结构特征及其结构与生理活性之间的潜在相互作用仍有待进一步明确。本文全面回顾了抗性糊精的制备方法、结构特性、健康益处和应用领域。此外,本文还预测了其未来的发展趋势。本综述的主要目的是为进一步研究、创新功能性产品和扩大抗性糊精的使用范围提供理论指导和新的视角。
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引用次数: 0
Influence of Sweeteners on the Phytochemical and Physicochemical Quality and Consumer Acceptability of Roselle Beverage 甜味剂对洛神花饮料的植物化学和物理化学质量以及消费者接受度的影响
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-16 DOI: 10.1155/2024/6669374
Nazir Kizzie-Hayford, Salifu Seidu-Larry, Shilla Owusu-Ansah, Bright Quaye, Jerry Ampofo-Asiama

Bissap is prepared from Roselle calyxes (Hibiscus sabdariffa L.) by hot infusion and marketed as a health drink. To improve the tart attributes, sucrose is usually added. However, because of nutrition and health concerns, processors explore other types of sweeteners, but the impact on the phytochemical and physicochemical quality and sensory properties of Bissap is not reported despite the potential influence on consumer acceptability. In this study, Roselle calyx extract was prepared to which sugarloaf pineapple pulp, Roscoe ginger, Negro/Ethiopian pepper, and cloves were added to obtain the Bissap stock (control). Then, either sucrose, caramel, honey, or sucralose was added to the Bissap to achieve a comparable sweetness (13.1°Brix), and the effects were assessed during storage. The results showed that caramel and honey significantly increased the nonenzymatic browning of Bissap from 0.49 ± 0.04 to 0.66 ± 0.07 and 0.64 ± 0.02, and the cloud value from 0.14 ± 0.01 to 0.23 ± 0.01 and 0.28 ± 0.02, respectively. The use of honey increased the ascorbic acid from 2.58 ± 0.17 to 3.35 ± 0.19 mg AE/mL, phenols from 11.25 ± 0.90 to 17.66 ± 1.07 mg GAE/mL, flavonoid from 15.33 ± 1.12 to 27.02 ± 1.69 mg QE/mL, and antioxidant capacity from 16.59 ± 1.34 to 25.36 ± 1.00 mg GAE/mL. During storage, ascorbate content decreased, but at a rate lower for honey-Bissap than the other sweeteners whilst the flavonoid and antioxidant activity of honey- and caramel-Bissap improved. The physicochemical changes led to a shelf life of 10 days at 6°C storage. Sensory analysis revealed the highest consumer (n = 75) acceptability scores for sucrose (5.89 ± 0.17), sucralose (5.43 ± 0.17), caramel (5.07 ± 0.17), honey (4.30 ± 0.20), and unsweetened Bissap (2.59 ± 0.22). Although honey enhanced the functional quality of Bissap, sucralose showed the highest potential as an alternative sweetener.

Bissap 是用洛神花萼(Hibiscus sabdariffa L.)通过热浸泡制成的,作为一种健康饮料在市场上销售。为了改善酸味,通常会添加蔗糖。然而,出于对营养和健康的考虑,加工商也在探索其他类型的甜味剂,但尽管对消费者接受度有潜在影响,但对 Bissap 的植物化学和物理化学质量及感官特性的影响却未见报道。在这项研究中,制备了洛神花萼提取物,并在其中加入糖叶菠萝浆、罗斯科生姜、黑人/埃塞俄比亚胡椒和丁香,以获得 Bissap 汤料(对照)。然后,在 Bissap 中加入蔗糖、焦糖、蜂蜜或三氯蔗糖,以达到相当的甜度(13.1°Brix),并在储存期间对其效果进行评估。结果表明,焦糖和蜂蜜分别使比萨普的非酶褐变从 0.49±0.04 显著增加到 0.66±0.07 和 0.64±0.02,云雾值从 0.14±0.01 显著增加到 0.23±0.01 和 0.28±0.02。使用蜂蜜后,抗坏血酸从 2.58±0.17 mg AE/mL 增加到 3.35±0.19 mg AE/mL,酚从 11.25±0.90 mg GAE/mL 增加到 17.66±1.07 mg GAE/mL,黄酮从 15.33±1.12 mg QE/mL 增加到 27.02±1.69 mg QE/mL,抗氧化能力从 16.59±1.34 mg GAE/mL 增加到 25.36±1.00 mg GAE/mL。在贮藏过程中,抗坏血酸含量有所下降,但蜂蜜-比萨普的下降速度低于其他甜味剂,而蜂蜜-比萨普和焦糖-比萨普的类黄酮和抗氧化活性有所提高。这些理化变化使得蜂蜜比萨普在 6°C 的储存条件下的保质期延长了 10 天。感官分析表明,消费者(n=75)对蔗糖(5.89±0.17)、三氯蔗糖(5.43±0.17)、焦糖(5.07±0.17)、蜂蜜(4.30±0.20)和无甜味比萨普(2.59±0.22)的可接受性评分最高。虽然蜂蜜提高了 Bissap 的功能品质,但蔗糖素(三氯蔗糖)作为替代甜味剂的潜力最大。
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引用次数: 0
Impact of Thermal, Ultrasonication, and Thermosonication Processes on the Quality Profile of Watermelon-Beetroot Juice Blend: A Comparative Study 热处理、超声波处理和热超声波处理对西瓜-甜菜根混合果汁质量的影响:比较研究
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-08 DOI: 10.1155/2024/5518914
Kinza Mukhtar, Brera Ghulam Nabi, Muhammad Faisal Manzoor, Sania Zia, Zuhaib F. Bhat, Shahzad Hussain, Rana Muhammad Aadil

Fruit juices are popular beverages that provide various health benefits due to their rich nutritional profile, but they are prone to microbial spoilage and quality deterioration. Thermal pasteurization is the conventional method to preserve fruit juices, but it causes undesirable changes in the physicochemical and nutritional value of the juices. Therefore, there is a need to develop alternative methods to ensure the microbial safety and quality of fruit juices. The aim of this study was to investigate the impact of thermal (95-100°C for 4 min), ultrasonication (US) (25 kHz for 5 and 10 min), and thermosonication (TS) (25 kHz at 40 and 50°C) processes on the quality profile of watermelon-beetroot juice blend, a novel juice formulation with enhanced nutritional and functional properties with 50 : 50 formulation. The samples were analysed for physicochemical (colour, pH, total soluble solids, and titratable acidity), bioactive (phenolic, flavonoid, antioxidant, and ascorbic acid contents), and microbiological (total plate count and yeast/molds) properties. The results showed that all the processed samples retained high total phenolic (756.33-842.33 μg GAE/g), total flavonoid (435.33-512.67 μg CE/g), and ascorbic acid (45.23-50.34 mg/100 mL) contents along with a high antioxidant potential (total antioxidant capacity (274.14-305.33 μg AAE/g) and DPPH radical scavenging activity (33.05-42.18%)) while preserving the normal physicochemical characteristics and decreasing the microbial counts of all the processed blend juices. In conclusion, the US treatment (10 min) produced the juice blends with the best quality. The findings of this research suggest that thermal, US, and TS processes are promising technologies for the preservation of fruit juices and that watermelon-beetroot juice blend is a novel juice formulation with high nutritional and functional value. The results of this research might be useful to the processed fruit juice industry and the consumers who are looking for healthy and safe fruit juices.

果汁是一种广受欢迎的饮料,其丰富的营养成分为人们的健康提供了各种益处,但果汁也很容易受到微生物的侵蚀而变质。热巴氏杀菌法是保存果汁的传统方法,但会导致果汁的理化和营养价值发生不良变化。因此,有必要开发其他方法来确保果汁的微生物安全和质量。本研究的目的是调查热处理(95-100°C,4 分钟)、超声波处理(25 千赫,5 分钟和 10 分钟)和热超声波处理(TS)(25 千赫,40°C 和 50°C)对西瓜-甜菜根混合果汁质量的影响:50 的配方。对样品进行了理化(颜色、pH 值、总可溶性固体和可滴定酸度)、生物活性(酚类、类黄酮、抗氧化剂和抗坏血酸含量)和微生物(总板计数和酵母/霉菌)特性分析。结果表明,所有加工过的样品都保留了较高的总酚(756.33-842.33 μg GAE/g)、总黄酮(435.33-512.67 μg CE/g)和抗坏血酸(45.23-50.34 mg/100 mL)含量,以及较高的抗氧化潜力(总抗氧化能力(274.14-305.33 μg AAE/g)和 DPPH 自由基清除活性(33.05-42.18%)),同时保持了所有加工混合果汁的正常理化特性并降低了微生物数量。总之,美国处理(10 分钟)生产的混合果汁质量最好。研究结果表明,热处理、US 处理和 TS 处理都是很有前景的果汁保存技术,西瓜-甜菜根混合果汁是一种新型果汁配方,具有很高的营养和功能价值。这项研究的结果可能会对加工果汁行业和追求健康安全果汁的消费者有所帮助。
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引用次数: 0
Co-encapsulation of β-D-Galactosidase and Ascorbic Acid in the Milk Protein-Based Microcapsules: Optimization and Characterization 牛奶蛋白基微胶囊中β-D-半乳糖苷酶和抗坏血酸的共包囊:优化与表征
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-04 DOI: 10.1155/2024/6646949
Mahmoud Hosseinnia, Mohammad Alizadeh Khaledabad, Hadi Almasi

This research is aimed at preparing the β-galactosidase (βg) and vitamin C (VC) cocapsules stabilized by milk proteins. The effect of different independent parameters including core-coating ratio (10-100%), whey protein isolate (0 : 1), sodium caseinate (0 : 1), and ultrasound power (50-150 W) on physicochemical properties of microcapsules was investigated. The response surface methodology (RSM) defined the optimal conditions. Increasing the WPI values had different effects on the particle size and polydispersity index (PDI). The zeta potential values decreased by decreasing SC values. The βg had better encapsulation efficiency in comparison to VC. Increasing the core-coating ratio showed a negative effect on the enzyme activity. Among the test parameters, the core-coating ratio was effective on the viscosity of microcapsules. Two optimum conditions for co-encapsulation were determined as WPI, SC, core-coating ratio, and ultrasound power of 0, 1, 100%, and 79.4 W and 0.2, 0.8, 100%, and 75 W for microcapsules I and II, respectively. In the next step, the structural and morphological properties of the optimum samples were analyzed. The heterogeneous morphology of microcapsules was observed by SEM analysis. The formation of new interactions between wall materials, βg, and VC was confirmed by FT-IR analysis. XRD analysis revealed that the WPI-coated sample had a higher crystallinity index. Generally, the successful co-encapsulation of βg and VC exhibited the potential of the resultant microcapsules for the industrial production of VC fortified and lactose-free milk.

本研究旨在制备由牛奶蛋白稳定的β-半乳糖苷酶(βg)和维生素C(VC)胶囊。研究了不同独立参数对微胶囊理化性质的影响,包括包芯率(10-100%)、分离乳清蛋白(0 : 1)、酪蛋白酸钠(0 : 1)和超声功率(50-150 W)。响应面方法(RSM)确定了最佳条件。增加 WPI 值对粒度和多分散指数(PDI)有不同的影响。随着 SC 值的降低,zeta 电位值也随之降低。与 VC 相比,βg 的封装效率更高。增加包芯比对酶活性有负面影响。在测试参数中,包芯比对微胶囊的粘度有影响。微胶囊 I 和微胶囊 II 的两个最佳共包囊条件分别为 WPI、SC、包芯比和超声功率,其中 WPI 为 0、1、100% 和 79.4 W,包芯比为 0.2、0.8、100% 和 75 W。接下来,分析了最佳样品的结构和形态特性。通过扫描电镜分析观察到了微胶囊的异质形态。傅立叶变换红外分析证实了壁材料、βg 和 VC 之间形成了新的相互作用。XRD 分析表明,WPI 包覆的样品具有更高的结晶度指数。总体而言,βg 和 VC 的成功共包覆表明,所制得的微胶囊具有工业化生产 VC 强化乳和无乳糖牛奶的潜力。
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引用次数: 0
Advancements in Food Printing Technologies and Their Potential Culinary Applications: A Contemporary Exploration 食品印刷技术的进步及其潜在的烹饪应用:当代探索
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-22 DOI: 10.1155/2024/6621344
Irtiqa Shabir, Aamir Hussain Dar, Sobiya Manzoor, Vinay Kumar Pandey, Shivangi Srivastava, Rafeeya Shams, Kshirod Kumar Dash, Iqra Bashir, Ufaq Fayaz, V. Prithviraj, Punit Singh, Sarvesh Rustagi, Seema Ramniwas, R. Pandiselvam

Food printing is a cutting-edge manufacturing technique that uses advanced printing methods such as binder jetting, extrusion-based printing, and inkjet printing to build an object layer by layer to achieve the required shape of food items such as chocolate and cheese. 3DFP (3-dimensional food printing) has the potential to combine delicate and easily degradable bioactive compounds and other functional elements into functional 3DFP food products, contributing greatly to the development of nutritious food. Many nations make different types of 3D food printers nowadays, creating specialty meals like space food, restaurants, elderly food, and floating food. Numerous benefits of 3DFP include the development of individualized food items with regard to taste and nutrition, the decentralisation of food production, the decrease of food waste, and commercial innovation. Based on the benefits of customizing current food to one’s taste and use, three-dimensional food printing technology can be applied to a variety of food categories. One of the reasons for the increase in research into this technology is the ability to produce modified products that are tailored to suit the taste preferences and specific nutritional demands of consumers. In this review, the industrial situation of 3DFP technology was examined along with recommendations for expanding the market for 3D-printed food in the new typical age.

食品印刷是一种尖端制造技术,它采用先进的印刷方法,如粘合剂喷射、挤出式印刷和喷墨印刷,逐层构建物体,以实现巧克力和奶酪等食品所需的形状。3DFP(三维食品打印)有可能将细腻、易降解的生物活性化合物和其他功能元素结合成功能性3DFP食品,为营养食品的开发做出巨大贡献。如今,许多国家都制造出了不同类型的三维食品打印机,制作出了太空食品、餐厅食品、老年食品和漂浮食品等特色食品。3DFP 的诸多益处包括:在口味和营养方面开发个性化食品、分散食品生产、减少食品浪费以及商业创新。基于根据个人口味和用途定制现有食品的好处,三维食品印刷技术可应用于各种食品类别。对这一技术的研究日益增多,原因之一是能够生产出适合消费者口味偏好和特定营养需求的改良产品。在这篇综述中,我们研究了 3DFP 技术的工业状况,并提出了在新典型时代扩大 3D 打印食品市场的建议。
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引用次数: 0
Optimization of Wall Materials for Astaxanthin Powder Production from Shrimp Shell Extract Using Simplex Lattice Mixture Design 利用简单网格混合物设计优化从虾壳提取物中生产虾青素粉末的壁材
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-21 DOI: 10.1155/2024/9794290
P. Sharayei, E. Azarpazhooh, S. Einafshar, Sh. Zomorodi, F. Zare, Hosahalli S. Ramaswamy

Shrimp shell waste is an attractive source of value-added bioactive-rich by-products. Shrimp shell extract containing astaxanthin was recovered by solvent extraction method (petroleum ether/acetone/water with a ratio of 15 : 75 : 10) and ultrasound process (amplitude 20% for 15 min at 35°C). The extract was then encapsulated by freeze-drying using wall materials such as maltodextrin (with the dextrose equivalent (DE) of 7 (MD7) and 20 (MD20)) and modified starch (Hi-Cap 100) incorporated at different ratios. Simplex lattice with augmented axial points in the mixture design was applied for the optimization of wall material. The optimal wall materials were 29.4% (MD7), 34.0% (Hi-Cap 100), and 36.6% (MD20), with encapsulation yield (Y) of 94.6%, encapsulation efficiency (EE) of 91.8%, astaxanthin content (Ast) of 46.1 μg/g DW, and DPPH scavenging capacity of 64.0%, respectively. The optimized microcapsules had spongy morphology and brittle and flaky mass. The degradation kinetics of bioactive astaxanthin in UV light was evaluated and found to follow first-order reaction kinetics. The microcapsules obtained under optimal wall composition exhibited the highest UV light stability with half-life values of 76.8 h, demonstrating a high stability.

虾壳废料是富含生物活性的高附加值副产品的诱人来源。含有虾青素的虾壳提取物是通过溶剂萃取法(石油醚/丙酮/水,比例为 15 : 75 : 10)和超声波法(振幅 20%,35°C,15 分钟)回收的。然后,使用麦芽糊精(葡萄糖当量(DE)为 7(MD7)和 20(MD20))和变性淀粉(Hi-Cap 100)等壁材,以不同比例对提取物进行冷冻干燥封装。在混合物设计中采用了带有增强轴点的简单网格来优化壁材。最佳壁材分别为 29.4%(MD7)、34.0%(Hi-Cap 100)和 36.6%(MD20),封装率(Y)为 94.6%,封装效率(EE)为 91.8%,虾青素含量(Ast)为 46.1 μg/g DW,DPPH 清除能力为 64.0%。优化后的微胶囊呈海绵状,质地脆而薄。对生物活性虾青素在紫外光下的降解动力学进行了评估,发现其遵循一阶反应动力学。在最佳壁成分下获得的微胶囊具有最高的紫外光稳定性,半衰期值为 76.8 h,显示出较高的稳定性。
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引用次数: 0
Variations of Carotenoids, Total Bioactive Contents, and Antioxidant Activity in Leaves of Medicago polymorpha 叶中类胡萝卜素、总生物活性成分和抗氧化活性的变化
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-21 DOI: 10.1155/2024/8721888
Alam Zeb, Khalil Ahmad

Medicago polymorpha L. (MP) is a herbaceous plant commonly known as “bur clover.” It is aboriginal to Central and Western Asia and extensively distributed around the world. This study determines the changes in carotenoids and antioxidant potential of different MP of different origins. The sample leaves were analyzed for identification and quantification of carotenoids using a reversed-phase HPLC system. The result showed nine carotenoids and pigments in each sample. The major carotenoid compounds were phytoene, phytofluene, neoxanthin and its isomer (9-Z-neoxanthin), violaxanthin, lutein along with their isomers (9-Z-lutein, 9′-Z-lutein), and all-E-β-carotene. The major pigments were 15-hydroxy-lactone chlorophyll a, pheophytin a, pheophytin a ′, pyropheophytin a, divinyl chlorophyll a, chlorophyll b ′, chlorophyll b, 13′-hydroxy-lactone chlorophyll b, and hydroxy pheophytin a ′. The carotenoids were detected in considerable amounts in the samples from Lower Dir (213 μg/g), Swat (171 μg/g), and Buner (157 μg/g). Chlorophylls were higher in Lower Dir (203.4 μg/g), Swat (184.0 μg/g), and Buner (152.2 μg/g) and significantly lower amounts in Malakand samples (141.7 μg/g). The total carotenoids in Lower Dir (51.2%) were higher than in Swat (48.2%), Buner (50.8%), and Malakand samples (44.6%). The amounts of violaxanthin and lutein were significantly higher in Lower Dir (73.6%) samples, followed by Malakand (51.7%), whereas Buner and Swat samples showed the lowest levels. In conclusion, MP leaves are a good source of important carotenoids having potential antioxidant properties, which are highly correlated to the violaxanthin and lutein contents.

多苜蓿(Medicago polymorpha L.,MP)是一种草本植物,俗称 "毛三叶草"。它原产于中亚和西亚,广泛分布于世界各地。本研究确定了不同产地的 MP 类胡萝卜素和抗氧化潜力的变化。采用反相高效液相色谱系统对样本叶片进行了类胡萝卜素的鉴定和定量分析。结果显示,每个样本中都含有九种类胡萝卜素和色素。主要类胡萝卜素化合物有植物烯、植物芴、新黄质及其异构体(9-Z-新黄质)、长叶黄质、叶黄素及其异构体(9-Z-叶黄素、9′-Z-叶黄素)和全-E-β-胡萝卜素。主要色素有 15-羟基内酯叶绿素 a、叶绿素 a、叶绿素 a′、焦叶绿素 a、二乙烯基叶绿素 a、叶绿素 b′、叶绿素 b、13′-羟基内酯叶绿素 b 和羟基叶绿素 a′。在下迪尔(213 微克/克)、斯瓦特(171 微克/克)和布内尔(157 微克/克)的样本中检测到大量类胡萝卜素。下迪尔(203.4 微克/克)、斯瓦特(184.0 微克/克)和布纳尔(152.2 微克/克)的叶绿素含量较高,而马拉坎德样本(141.7 微克/克)的叶绿素含量明显较低。下迪尔(51.2%)的类胡萝卜素总量高于斯瓦特(48.2%)、布纳尔(50.8%)和马拉坎德(44.6%)。下迪尔(73.6%)样本中的小黄素和叶黄素含量明显较高,其次是马拉坎德(51.7%),而布内尔和斯瓦特样本中的含量最低。总之,MP 树叶是重要类胡萝卜素的良好来源,具有潜在的抗氧化特性,这与叶黄素和叶黄素的含量高度相关。
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引用次数: 0
Face-Centered Central Composite Design for the Optimization of the Extraction of Phenolic Compounds from Kernels and Shells of Raphia farinifera and Evaluation of the Antioxidant, Antimicrobial, and Anti-Inflammatory Activities 面心中心复合设计用于优化从 Raphia farinifera 果核和果壳中提取酚类化合物并评估其抗氧化、抗菌和抗炎活性
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-21 DOI: 10.1155/2024/8849005
Paul Alain Nanssou Kouteu, Aurelie Gislaine Kemegne, Majeste Mbiada Pahane, Adeline Sabine Yadang Fanta, Amandine Kofane Membangmi, Donald Vivien Toukak Tchamaleu, Gabriel Agbor Agbor, Martin Ruben Mouangue

This study reports the extraction of phenolic compound from shells and kernels of the Raphia farinifera fruit and the biological activities of the extract. A face-centered composite design was established to optimize the extraction conditions: ethanol/water ratio (0-100%), solvent/powder ratio (10-30 mL/g), and extraction time (90-180 min). Subsequently, the extracts obtained under the optimal conditions were used for the evaluation of the radical scavenging capacity, the capacity to chelate ferric ions, and the antimicrobial and anti-inflammatory activities. The optimal extraction conditions for the shells are an extraction time of 134.24 min, 65% ethanol in water, and a solvent/substrate ratio of 21.16 mL/g, and for the kernel, an extraction time of 180 min, 94.3% ethanol in water, and a solvent/substrate ratio of 18.6 mL/g. In these conditions, the phenolic compounds were 95.36 mg EAG/L for the shell extract and 139.72 mg EAG/L for the kernel extract. The antioxidant activity revealed that the half-maximal inhibitory concentrations (IC50) of the kernel extracts are 22.32 μg/mL and 55.73 μg/mL for the shells. A reducing activity of Fe 3+ ions with an activity of 308.39 μg EAA/mg for the kernel extracts and 293 μg EAA/mg for the shells was observed. B. cereus was the most sensitive microorganism with a minimum inhibitory concentration (MIC) equal to the minimum bactericidal concentration (MBC) with a value of 156.25 ppm for the kernel extract while the shell extract showed MIC of 625 ppm and MBC of 2500 ppm. The IC50 values for the denaturation of proteins by extracts of shells and kernels are 0.76 μg/mL and 0.56 μg/mL, respectively. Membrane stabilization revealed IC50 values of 1054.54 μg/mL and 1339 μg/mL for the shell and kernel extracts, respectively. This work has shown the potential of Raphia farinifera extracts for the food industry and cosmetics.

本研究报告了从远志果的果壳和果核中提取酚类化合物及其生物活性的情况。研究采用面心复合设计法优化了萃取条件:乙醇/水比例(0-100%)、溶剂/粉末比例(10-30 mL/g)和萃取时间(90-180 min)。随后,在最佳条件下获得的提取物被用于评估自由基清除能力、铁离子螯合能力以及抗菌和抗炎活性。果壳的最佳萃取条件为:萃取时间 134.24 分钟,乙醇水溶液浓度 65%,溶剂/底物比 21.16 mL/g;果核的最佳萃取条件为:萃取时间 180 分钟,乙醇水溶液浓度 94.3%,溶剂/底物比 18.6 mL/g。在这些条件下,果壳提取物的酚类化合物含量为 95.36 mg EAG/L,果核提取物的酚类化合物含量为 139.72 mg EAG/L。抗氧化活性显示,核提取物的半最大抑制浓度(IC50)为 22.32 μg/mL,壳提取物为 55.73 μg/mL。果仁提取物对 Fe 3+ 离子的还原活性为 308.39 μg EAA/mg,果壳为 293 μg EAA/mg。蜡样芽孢杆菌是最敏感的微生物,其最小抑菌浓度(MIC)等于最小杀菌浓度(MBC),果仁提取物的最小抑菌浓度为 156.25 ppm,而果壳提取物的最小抑菌浓度为 625 ppm,最小杀菌浓度为 2500 ppm。果壳和果核提取物变性蛋白质的 IC50 值分别为 0.76 μg/mL 和 0.56 μg/mL。膜稳定性显示,壳和核提取物的 IC50 值分别为 1054.54 μg/mL 和 1339 μg/mL。这项工作显示了远志萘提取物在食品工业和化妆品方面的潜力。
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引用次数: 0
Potential of β-Amylase from Sweet Potato (Ipomoea batatas Lam) Extract on the Mashing of Safrari Sorghum 甘薯(Ipomoea batatas Lam)提取物中的β-淀粉酶对 Safrari 高粱粉碎的潜力
IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-03-19 DOI: 10.1155/2024/6629417
Daruis Gayus Kameugne Limegne, Steve Carly Zangue Desobgo, Emmanuel Jong Nso

The aim of this study was to investigate the use of sweet potato as a local source of enzymatic extract for the saccharification of sorghum mash. Box-Behnken designs were employed to determine the optimal conditions for extracting crude enzymes and saccharifying Safrari sorghum mash. The optimal conditions for maximizing enzymatic activity were found to be a mass-to-volume ratio of 0.1, an extraction time of 210 min, and a temperature of 60°C. The theoretical and experimental enzymatic activities under these conditions were 23.83 U/mg and 23.49 U/mg, respectively. The extraction of enzymes under these optimal conditions resulted in wort with physicochemical parameters within the following ranges: turbidity (0.79 to 4.52 NTU), pH (5.40 to 8.85), brix (14.80 to 17.50°B), reducing sugars (0.17 to 0.2114 mg/mL), and titratable acidity (3.54 to 5.24 g/L). These findings demonstrate that the extract from Ipomoea batatas contains enzymes that can be effectively used in the mashing process of malted Safrari sorghum.

本研究的目的是调查甘薯作为当地酶提取物的一种来源,用于高粱醪的糖化。研究采用箱-贝肯设计法确定提取粗酶和糖化 Safrari 高粱醪的最佳条件。结果发现,使酶活性最大化的最佳条件是质量体积比为 0.1、提取时间为 210 分钟、温度为 60°C。在这些条件下,理论和实验酶活性分别为 23.83 U/mg 和 23.49 U/mg 。在这些最佳条件下提取酶后,麦汁的理化参数在以下范围内:浊度(0.79 至 4.52 NTU)、pH 值(5.40 至 8.85)、糖度(14.80 至 17.50°B)、还原糖(0.17 至 0.2114 mg/mL)和可滴定酸度(3.54 至 5.24 g/L)。这些研究结果表明,蝙蝠苕提取物中含有的酶可有效地用于发芽 Safrari 高粱的糖化过程。
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引用次数: 0
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Journal of Food Processing and Preservation
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