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Fermentation Kinetics and Physicochemical Characterization of Nonalcoholic Wine Produced From Cashew Apple Juice 用腰果苹果汁酿制的无酒精葡萄酒的发酵动力学和理化特征
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-23 DOI: 10.1155/2024/3071071
Yaw Gyau Akyereko, Faustina Dufie Wireko-Manu, Francis Alemawor, Mary Adzanyo

There is an increasing demand for nonalcoholic wines owing to religious beliefs, responsible driving, harmful effects of alcohol, and strict alcohol regulatory measures. Alcohol removal methods, used for nonalcoholic wine production, have negative impact on flavour characteristics and consumer acceptance. This study seeks to determine the fermentation kinetics and physicochemical properties of nonalcoholic wine developed from cashew apple juice using cold fermentation in combination with special yeast. The extracted cashew apple must was inoculated with S. ludwigii and Z. rouxii yeast culture separately and allowed to ferment at 0°C–4°C for 7 days in a completely randomized design. The pH, total soluble solids (TSS), temperature, specific gravity, titratable, volatile, and nonvolatile acidities of the fermenting must and wine were determined using standard methods. All the yeast strains maintained comparable fermentation trends with respect to pH, TSS, specific gravity, volatile acidity, titratable acidity, and fixed acid, but with different alcohol yields of 0.89 and 0.68% for S. ludwigii and Z. rouxii, respectively. The wine produced using S. ludwigii had a lower reducing sugar (3.58 mg/L) and TSS (6.65°Brix) compared to Z. rouxii (6.23 mg/L and 7.65°Brix), respectively. However, no significant differences existed in pH, titratable acidity, specific gravity, alcohol content, and colour characteristics: brightness (L). The matured wine products recorded 0% alcohol content and had distinct appealing colours. The application of cold fermentation combined with special yeast in nonalcoholic wine production is promising and deserves more research to explore its full potential in meeting the increasing demand for nonalcoholic wines.

由于宗教信仰、负责任的驾驶、酒精的有害影响以及严格的酒精监管措施,人们对无酒精葡萄酒的需求日益增长。无醇葡萄酒生产中使用的脱醇方法会对风味特征和消费者接受度产生负面影响。本研究旨在确定腰果苹果汁结合特殊酵母冷发酵法酿制的无酒精葡萄酒的发酵动力学和理化特性。将提取的腰果苹果汁分别接种 S. ludwigii 和 Z. rouxii 酵母菌培养物,在 0°C-4°C 温度下发酵 7 天,采用完全随机设计。发酵葡萄汁和葡萄酒的 pH 值、总可溶性固形物(TSS)、温度、比重、可滴定酸度、挥发性酸度和非挥发性酸度均采用标准方法测定。所有酵母菌株在 pH 值、TSS、比重、挥发性酸度、可滴定酸度和固定酸度方面都保持了相似的发酵趋势,但 S. ludwigii 和 Z. rouxii 的酒精产量不同,分别为 0.89% 和 0.68%。与胭脂虫(6.23 mg/L 和 7.65°Brix)相比,使用鲁德维希藻酿造的葡萄酒还原糖(3.58 mg/L)和 TSS(6.65°Brix)较低。然而,在 pH 值、可滴定酸度、比重、酒精含量和颜色特征:亮度(L∗)方面没有明显差异。熟化葡萄酒的酒精含量为 0%,颜色明显诱人。冷发酵与特殊酵母在无酒精葡萄酒生产中的应用前景广阔,值得进一步研究,以充分挖掘其潜力,满足人们对无酒精葡萄酒日益增长的需求。
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引用次数: 0
Determination of the Effect of Different Energy Classes of Household Refrigerators on the Freezing Process and the Quality Characteristics of Beef 测定家用冰箱的不同能级对牛肉冷冻过程和质量特性的影响
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-21 DOI: 10.1155/2024/9483029
Hira Yüksel Sarıoğlu, Safiye Nur Dirim

The household refrigerator model used for freezing processes in this study was produced in the factory in different energy classes (C, D, and E). Equal amounts of beef (10 × 10 × 2 cm; 2 × 2 × 2 cm) and minced beef (10 × 10 × 2 cm) were placed in refrigerator bags before freezing. The freezing processes were carried out by placing beef samples on the top shelf where the radial fan was located in the freezer compartment of the refrigerators. After the frozen samples were thawed at +4°C, they were cooked in a water bath at 85°C. It was determined that the samples frozen in the refrigerator belonging to the D energy class at −24°C had shorter freezing times and therefore higher freezing rates. It was observed that pH (5.54–5.72) values of samples frozen in the D energy class were higher due to lower weight loss (0.32%–6.80%) values. When their color values were examined, the redness (a) values (15.09–17.61) were the highest, while the yellowness (b) values (10.31–13.29) were the lowest in these samples. In addition, the hardness (49.00–182.74) and chewiness (16.35–71.80) values of samples frozen in Energy Class D were found to be lower, while their protein denaturation temperatures were higher due to shorter freezing times. Consequently, in this study, the effects of the difference in energy classes of refrigerators on the quality characteristics of beef samples were examined, and the refrigerator belonging to the energy class (D) that gave the best quality properties was found to be the most suitable one for freezing the beef.

本研究中用于冷冻过程的家用冰箱型号是由工厂生产的不同能量等级(C、D 和 E)的冰箱。在冷冻前,将等量的牛肉(10 × 10 × 2 厘米;2 × 2 × 2 厘米)和碎牛肉(10 × 10 × 2 厘米)放入冷冻袋中。在冷冻过程中,将牛肉样品放在冰箱冷冻室中径向风扇所在的顶层。冷冻样品在 +4°C 解冻后,在 85°C 的水浴中煮熟。结果表明,在属于 D 能级的冰箱中-24℃冷冻的样品冷冻时间较短,因此冷冻率较高。据观察,在 D 能级中冷冻的样品的 pH 值(5.54-5.72)较高,而重量损失值(0.32%-6.80%)较低。在检测色度值时,这些样品的红度(a∗)值(15.09-17.61)最高,而黄度(b∗)值(10.31-13.29)最低。此外,在能量等级 D 中冷冻的样品硬度(49.00-182.74)和咀嚼度(16.35-71.80)值较低,而由于冷冻时间较短,其蛋白质变性温度较高。因此,本研究考察了不同能量等级的冰箱对牛肉样本质量特性的影响,发现属于能量等级(D)的冰箱能提供最好的质量特性,是最适合冷冻牛肉的冰箱。
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引用次数: 0
Influence of 1-Methylcyclopropene and Geranium Oil Treatments on Oxidative Stress and Antioxidant Enzyme Activity in Carica papaya L. During Cold Storage 1-甲基环丙烯和天竺葵油处理对冷藏期间木瓜氧化应激和抗氧化酶活性的影响
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-19 DOI: 10.1155/2024/7458405
Meng Yi Loh, Noranizan Mohd Adzahan, Ezzat Mohamad Azman, Soo Peng Koh, Noor Liyana Yusof

Carica papaya Linn, known for its limited shelf life, typically relies on conventional cold storage (CS) methods to prolong freshness. However, being a fruit sensitive to chilling, papaya exhibits low tolerance to cold conditions, making it susceptible to chilling injury during CS. This study was undertaken to explore the correlation between postharvest treatment using 1-methylcyclopropene (1-MCP) and geranium oil (GO) and the antioxidant responses of papaya against oxidative stress during CS. Various treatments were administered, incorporating different concentrations and durations of exposure. The specified concentrations for 1-MCP and GO were 300 and 600 mg/L, and 2% and 4% (v/v), respectively, with exposure durations of 30 and 60 min. Over a 16-day period, the fruits underwent assessments for antioxidant activity, related enzyme activities, hydrogen peroxide content, and superoxide anion production rate at 4-day intervals. Both 1-MCP and GO treatments enhanced total phenolic and total flavonoid content, DPPH scavenging antioxidant capacity, and notably improved the activities of SOD, CAT, APX, and POD enzymes. This enhancement in antioxidant capacity and enzyme activities effectively mitigated the rise in reactive oxygen species levels in the fruits. The study revealed that 1-MCP treatment with higher doses and longer exposure times yielded superior outcomes, while GO treatment with higher doses and shorter exposure times produced favorable results compared to longer exposure times. Considering cost-effectiveness and time efficiency, a more comprehensive exploration of the synergistic effects of 1-MCP and GO may be essential to optimize effective doses within a reduced exposure timeframe.

木瓜(Carica papaya Linn)因其有限的货架期而闻名,通常依靠传统的冷藏(CS)方法来延长保鲜期。然而,作为一种对寒冷敏感的水果,木瓜对寒冷条件的耐受性很低,因此在冷藏过程中很容易受到冷害。本研究旨在探索使用 1-甲基环丙烯(1-MCP)和天竺葵油(GO)进行采后处理与木瓜在冷藏期间对氧化应激的抗氧化反应之间的相关性。处理方法多种多样,包括不同的浓度和接触时间。1-MCP 和 GO 的指定浓度分别为 300 毫克/升和 600 毫克/升,以及 2% 和 4% (v/v),接触时间分别为 30 分钟和 60 分钟。在 16 天的时间里,每隔 4 天对水果的抗氧化活性、相关酶活性、过氧化氢含量和超氧阴离子产生率进行一次评估。1-MCP 和 GO 处理都提高了总酚和总黄酮含量、DPPH 清除抗氧化能力,并显著改善了 SOD、CAT、APX 和 POD 酶的活性。抗氧化能力和酶活性的提高有效缓解了水果中活性氧水平的上升。研究显示,1-氯丙醇处理剂量越大、接触时间越长,效果越好,而 GO 处理剂量越大、接触时间越短,效果比接触时间越长越好。考虑到成本效益和时间效率,必须对 1-MCP 和 GO 的协同效应进行更全面的探索,以便在更短的暴露时间内优化有效剂量。
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引用次数: 0
Preservative Attributes of Near-Neutral Electrolyzed Water (NNEW) to Improve Shelf Life and Quality of Fresh and Minimally Processed Food: A Systematic Review 近中性电解水(NNEW)的防腐特性可提高新鲜食品和微加工食品的保质期和质量:系统综述
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-19 DOI: 10.1155/2024/1650453
Andrés Rivera-García, Brenda A. Paz-Michel

Perishable foods are characterized by a short shelf life, which is an important challenge for the companies that commercialize them. For this reason, the food industry keeps searching for new technologies to preserve their freshness, such as electrolyzed water. This review focuses on near-neutral electrolyzed water (NNEW) with a pH of 5.0–7.5, which has no negative impact on organoleptic characteristics nor on the nutritional quality of foods. It also examines how NNEW can be used to preserve fresh and minimally processed foods, detailing the extension of shelf life after treatments, its effects on food quality (e.g., appearance, texture, and color), and its potential mechanisms for controlling autolytic activity and spoilage microorganisms. Evidence of the attributes of NNEW as a food preservative, besides its well-known sanitizing properties, to extend the shelf life of fresh and MP edible plants and animal products is offered. The review further highlights practical applications for different types of food, including meat, seafood, fruits, and vegetables, and explores how NNEW could be integrated into handling, packaging, and distribution processes. Lastly, the need for large-scale industrial studies is emphasized to determine the feasibility of implementing the use of NNEW to reduce losses in food production and processing facilities, food supply chains, and retail.

易腐食品的特点是保质期短,这对于将其商业化的公司来说是一个重要的挑战。因此,食品行业一直在寻找新的保鲜技术,如电解水。本综述重点介绍 pH 值为 5.0-7.5 的近中性电解水(NNEW),它不会对食品的感官特性或营养质量产生负面影响。本研究还探讨了如何利用近中性电解水保存新鲜食品和微加工食品,详细介绍了近中性电解水经处理后可延长保质期、对食品质量(如外观、质地和色泽)的影响,以及其控制自溶活性和腐败微生物的潜在机制。除了众所周知的杀菌特性外,该综述还提供了萘乙酮作为食品防腐剂的特性证据,以延长新鲜和MP食用植物和动物产品的保质期。综述进一步强调了不同类型食品(包括肉类、海鲜、水果和蔬菜)的实际应用,并探讨了如何将 NNEW 融入处理、包装和分销流程。最后,还强调了进行大规模工业研究的必要性,以确定使用非核化生化武器减少食品生产和加工设施、食品供应链和零售业损失的可行性。
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引用次数: 0
Optimisation of Lutein Extraction From Gac (Momordica cochinchinensis) Fruit Peel and Evaluation of In Vitro Eye Protective Effects of the Extracts 优化嘎查(Momordica cochinchinensis)果皮中叶黄素的提取并评估提取物的体外眼保护作用
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-15 DOI: 10.1155/2024/3594099
Dao Nguyen, Thilini Thrimawithana, Terry Piva, Tien Huynh

Lutein is a macular biopigment that can protect the retina from oxidative stress induced by chronic exposure to light and oxidants. The peel of gac fruit (Momordica cochinchinensis) contains high levels of lutein, but the extraction optimisation parameters are complex, requiring saponification, and the effects on ocular bioactivity are not well understood. This study is aimed at optimising parameters for lutein extraction validated using HPLC from gac peel to determine their protective effects on oxidatively stressed retinal cells. The factors investigated for optimisation were KOH concentration, time, and temperature using (1) single factor, (2) multiple factor response surface methodology, and (3) predictive modelling. The optimum conditions for lutein extraction using single factors were 9% KOH for 3 h at 60°C, multiple factors were 9% KOH for 3 h at 45°C, and predictive modelling was 18% KOH for 1.9 h at 39.9°C, with the maximum extraction of lutein at 12.37, 15.64, and 17.4 mg/g DW, respectively. The extracted lutein purity and chemical composition were compared using HPLC and FTIR and indicated high purity and similarities in all extracts including the commercially purified lutein. Lutein extracted exhibited benefits on oxidatively stressed retinal pigment epithelium (RPE) cells that reversed damage to baseline unstressed levels exceeding 80% viability, similar to purified lutein standards. The feasibility of gac lutein and extraction optimisation were discussed for commercial translational opportunities as a medicinal bioproduct with a focus on eye benefits.

叶黄素是一种黄斑生物色素,可以保护视网膜免受长期暴露于光线和氧化剂所引起的氧化应激。槐果(Momordica cochinchinensis)的果皮含有大量叶黄素,但提取的优化参数很复杂,需要皂化,而且对眼部生物活性的影响也不甚了解。本研究旨在通过高效液相色谱法验证叶黄素提取的优化参数,以确定其对氧化应激视网膜细胞的保护作用。采用(1)单因素、(2)多因素响应面方法和(3)预测模型对 KOH 浓度、时间和温度等因素进行了优化。单因素叶黄素提取的最佳条件是 9% KOH,60°C,3 小时;多因素叶黄素提取的最佳条件是 9% KOH,45°C,3 小时;预测模型叶黄素提取的最佳条件是 18% KOH,39.9°C,1.9 小时,叶黄素的最大提取率分别为 12.37、15.64 和 17.4 mg/g DW。使用高效液相色谱法和傅立叶变换红外光谱法比较了提取叶黄素的纯度和化学成分,结果表明所有提取物(包括市售纯化叶黄素)的纯度都很高,且具有相似性。提取的叶黄素对氧化应激的视网膜色素上皮(RPE)细胞有益处,可将损伤逆转到基线非应激水平,存活率超过 80%,与纯化的叶黄素标准相似。会上还讨论了加克叶黄素的可行性和提取优化问题,以寻找作为药用生物产品的商业转化机会,重点关注对眼睛的益处。
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引用次数: 0
Effect of Cold Plasma-Treated Turmeric on the Oxidative Stability and Quality of the Oil From the Milk Thistle Seeds 冷等离子体处理姜黄对乳蓟籽油氧化稳定性和质量的影响
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-14 DOI: 10.1155/2024/3011121
Kamyab Samandari, Bahram Fathi-Achachlouei, Sodeif Azadmard-Damirchi, Jafar Borhanian, Ebrahim Taghinezhad, Antoni Szumny

Many studies have been carried out on the antioxidant effects of medicinal plants in edible oils. One of those plants is turmeric, which, due to its biological and antioxidant compounds such as curcuminoids, has a strong antioxidant effect and prevents oxidation of unsaturated fatty acids (FAs). In this study, turmeric at different levels (5% and 10% w/w) was used to increase the oxidative stability of the milk thistle (Silybum marianum L.) seed oil (MTSO). Additionally, to improve the performance of turmeric, cold plasma (CP) treatment was used at different durations (5 and 10 min). The effect of turmeric on the quality parameters of MTSO was investigated during different storage times (1, 30, and 60 days). The results indicated that the addition of turmeric treated with CP significantly (p < 0.05) decreased the acidity value (AV) (1.00–0.62 mg NaOH/g oil) and the peroxide value (PV) (4.47–3.65 mEq O2/kg oil) and also increased the total phenolic content (1003.23–1116.90 mg/GAE/100 g oil), antioxidant activity, α-tocopherol, and finally the oxidative stability compared to the control sample and oil containing untreated turmeric. Furthermore, gas chromatography (GC) analysis of the oil sample of the FA profile containing CP-treated turmeric revealed the higher ratio of unsaturated FAs (i.e., linoleic and arachidonic acid) and lower saturated FA content (palmitic and stearic acid) compared to oil samples containing untreated turmeric. To conclude, CP treatment increased turmeric performance and improved the quality characteristics of MTSO.

有关食用油中药用植物抗氧化作用的研究很多。姜黄就是其中的一种,姜黄中的姜黄素等生物抗氧化化合物具有很强的抗氧化作用,能防止不饱和脂肪酸(FA)氧化。在这项研究中,不同含量(5% 和 10% w/w)的姜黄被用来提高奶蓟草(Silybum marianum L.)种子油(MTSO)的氧化稳定性。此外,为了改善姜黄的性能,还采用了不同持续时间(5 分钟和 10 分钟)的冷等离子体(CP)处理。研究了姜黄在不同储存时间(1 天、30 天和 60 天)内对 MTSO 质量参数的影响。结果表明,与对照样品和含有未处理姜黄的油相比,添加经 CP 处理的姜黄能显著降低酸度值(AV)(1.00-0.62 mg NaOH/g)和过氧化值(PV)(4.47-3.65 mEq O2/kg 油)(p <0.05),还能提高总酚含量(1003.23-1116.90 mg/GAE/100 g 油)、抗氧化活性、α-生育酚以及氧化稳定性。此外,与含有未处理姜黄的油样相比,气相色谱(GC)分析显示,含有经氯化石蜡处理的姜黄的油样中不饱和脂肪酸(即亚油酸和花生四烯酸)的比例较高,而饱和脂肪酸(棕榈酸和硬脂酸)的含量较低。总之,氯化石蜡处理提高了姜黄的性能,改善了 MTSO 的质量特性。
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引用次数: 0
An Investigation Into Properties of Foam-Mat-Dried Spinach Powder and Physical Properties of Spinach Cube 对泡沫垫干菠菜粉的特性和菠菜块的物理特性的研究
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-14 DOI: 10.1155/2024/9114105
Maryam Sadat Emami, Mohebbat Mohebbi, Mohammad Khalilian-Movahhed

Today, producing fruit or vegetable cubes with high solubility and dispersibility can attract consumers’ attention, diminish agricultural waste, and raise people’s access to important nutritional sources. In recent years, it seems that cube-making preserves the appearance and marketability of food. In this study, the physicochemical properties of foam-mat-dried spinach powders with thicknesses of 3 and 5 mm produced at 40°C, 50°C, 60°C, and 70°C were investigated. The resulting powder was used to produce spinach cubes. The physicochemical properties of the powders were evaluated. With an increase in foam thickness, the water activity, moisture content, dissolution time, particle size, density, and porosity increased, whereas the hygroscopicity and glass transition temperature decreased. The lowest chlorophyll content (0.166 mg/g spinach) was observed in samples dried at 70°C and 5 mm. The samples dried at 70°C and 3 mm had the highest L and higher stability (lower moisture content and water activity and higher Tg). The Heckel equation showed that the highest k value was related to the spinach powder produced at 70°C and 5 mm. The cubes with the greatest heights, the highest chlorophyll content (0.245 mg/g spinach), and the lowest dissolution time were obtained from powders produced at 40°C and 3 mm. The cube of the powder produced at 70°C and 5 mm had the longest dissolution time. All cubes had a non-Fickian release in both dissolution media. The product of this research is dried spinach powder and its cubes. This product provides easy access to the valuable nutritional compounds of spinach in all conditions. This product is used as an additive in soup. It can also be used with yogurt and smoothies.

如今,生产具有高溶解性和高分散性的水果或蔬菜立方体可以吸引消费者的注意力,减少农业废弃物,提高人们获得重要营养来源的机会。近年来,立方体的制作似乎可以保持食品的外观和适销性。本研究调查了在 40°C、50°C、60°C 和 70°C 温度条件下生产的厚度分别为 3 毫米和 5 毫米的泡沫垫干燥菠菜粉的物理化学特性。所得粉末用于生产菠菜块。对粉末的物理化学特性进行了评估。随着泡沫厚度的增加,水活性、含水量、溶解时间、粒度、密度和孔隙率都有所增加,而吸湿性和玻璃化温度则有所下降。在 70°C 和 5 毫米条件下干燥的样品叶绿素含量最低(0.166 毫克/克菠菜)。在 70°C 和 3 毫米条件下干燥的样品具有最高的 L∗ 和更高的稳定性(更低的含水量和水活性以及更高的 Tg)。赫克尔方程显示,在 70°C 和 5 毫米条件下生产的菠菜粉 k 值最高。高度最大、叶绿素含量最高(0.245 毫克/克菠菜)、溶解时间最短的立方体来自 40°C 和 3 毫米条件下生产的粉末。在 70°C 和 5 毫米条件下生产的粉末立方体溶解时间最长。所有立方体在两种溶解介质中都有非菲氏释放。这项研究的产品是菠菜干粉及其立方体。该产品可让人们在任何条件下都能轻松获得菠菜中宝贵的营养成分。该产品可用作汤的添加剂。它还可以与酸奶和冰沙一起使用。
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引用次数: 0
Dynamic Changes in Physicochemical Properties, Amino Acid Content, and Flavour-Related Substances During Fortified Fermentation of Rice Wine With Saccharomycopsis fibuligera 酵母菌强化发酵米酒过程中理化性质、氨基酸含量和风味物质的动态变化
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-11 DOI: 10.1155/2024/5581068
Minghong Bian, Yuli Fang, Suhong Yang, Tianmeng Yuan, Xianjiang Zhou, Qiang Xu, Dongcai Jiang, Baolin Han

In recent years, the quality of rice wine has been greatly improved by multibacterial fermentation. In this study, Saccharomycopsis fibuligera (Sf) was selected as the experimental material to perform intensive fermentation of northeastern round glutinous rice (Db) and Yunzihong No. 23 (Zh). Anqi rice wine koji (Rl) served as the fermenting agent. The differences in the quality of rice wine before and after the addition of snap capsule-covering yeast were compared by determining the dynamic changes in the physicochemical properties, amino acids, and flavour-related compounds during rice wine fermentation. The results showed that adding Sf significantly (p < 0.05) increased the contents of alcohol, organic acids, and amino acids and imparted a new characteristic flavour to rice wine, resulting in more intense caramel and vinegar flavours. Overall, these results offer baseline information for using Sf in rice wine fermentation.

近年来,多菌发酵大大提高了米酒的品质。本研究选择纤维酵母菌(Sf)作为实验材料,对东北圆糯米(Db)和云子红 23 号(Zh)进行强化发酵。安琪米酒麴(Rl)作为发酵剂。通过测定米酒发酵过程中理化性质、氨基酸和风味相关化合物的动态变化,比较了添加扣盖酵母前后米酒质量的差异。结果表明,添加 Sf 能显著提高酒精、有机酸和氨基酸的含量(p < 0.05),并赋予米酒新的特征风味,使其具有更浓郁的焦糖和醋味。总之,这些结果为在米酒发酵中使用 Sf 提供了基本信息。
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引用次数: 0
Identification and Antibiotic Resistance of Lactobacillus and Bifidobacterium Species From Manufactured Probiotic Dairy Products 人造益生菌乳制品中乳酸杆菌和双歧杆菌的鉴定与抗生素耐药性
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-10 DOI: 10.1155/2024/1619353
Seda Seyirt, Başar Uymaz Tezel, Pınar Şanlıbaba

The objective of this study was to enumerate and identify Lactobacillus spp. and Bifidobacterium spp. from 70 samples of commercial dairy probiotic products and assess the antibiotic resistance profile of these probiotic cultures. Viable Lactobacillus spp. and Bifidobacterium spp. were not detected in 13 of 70 probiotic dairy products. Fifty-six products with specific claims of viable bacteria met or exceeded their label claim. Only one of the tested products had a lower number of viable bacteria than stated on the label. Based on the label claims, 11 samples explicitly indicated the presence of monoculture Bifidobacterium spp. strains. Meanwhile, 36 samples were labelled as containing a combination of Lactobacillus spp. and Bifidobacterium spp. species. However, 23 samples did not provide detailed information about the specific probiotic species present. The disc diffusion method was used for determining antibiotic resistance. High levels of resistance to methicillin (100%), vancomycin (95.3%), and cefoxitin (90.7%) were observed in Lactobacillus spp. strains. Bifidobacterium spp. strains were determined to have high frequencies of resistance to cefoxitin (100%), vancomycin (100%), and methicillin (95.6%). Finally, MDR rates were found to be 100% in Bifidobacterium and 95.3% in Lactobacillus species. Antibiotic resistance should be regarded as a significant part of the safety assessment of probiotics. Novel approaches will be essential for addressing MDR bacteria.

本研究的目的是从 70 个商业乳制品益生菌产品样本中计数和鉴定乳杆菌属和双歧杆菌属,并评估这些益生菌培养物的抗生素耐药性概况。在 70 种益生菌乳制品中,有 13 种未检测到可存活的乳酸杆菌和双歧杆菌。有 56 种产品的活性菌达到或超过了标签上的要求。只有一种受检产品的存活细菌数量低于标签上的规定。根据标签声明,11 个样品明确表示含有单培养双歧杆菌属菌株。同时,有 36 个样本标明含有乳酸杆菌和双歧杆菌的混合菌种。不过,有 23 个样本没有提供有关具体益生菌种类的详细信息。抗生素耐药性的测定采用了盘扩散法。乳杆菌属菌株对甲氧西林(100%)、万古霉素(95.3%)和头孢西丁(90.7%)的耐药性较高。双歧杆菌属菌株对头孢西丁(100%)、万古霉素(100%)和甲氧西林(95.6%)的耐药性频率较高。最后,双歧杆菌和乳酸杆菌的耐药率分别为 100%和 95.3%。抗生素耐药性应被视为益生菌安全性评估的重要组成部分。新方法对于解决耐药菌问题至关重要。
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引用次数: 0
Nutritious Instant Powder Production by Extrusion Processing Using Whole Oat and Broken Rice Flour 用全燕麦和碎米粉通过挤压加工生产营养速溶粉
IF 2 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-08 DOI: 10.1155/2024/4980863
Somayeh Okhravi, Elnaz Milani, Arash Koocheki

The effects of feed moisture content of 23% and 27%; die temperature of 120°C, 145°C, and 170°C; whole oat flour (WOF); and broken rice flour (BRF) at different ratios of 25:75, 50:50, and 75:25 were studied on functional and technological properties of the extrudates. Results showed that EI, hardness, porosity, and WAI were negatively affected by increasing WOF content, while this trend had a positive effect on WSI. Higher temperature resulted in higher WAI and WSI, but EI and hardness did not change with the amount of thermal treatment. Increasing the feed moisture content had a significant effect on elevating BD, hardness, and WAI and decreasing EI, porosity, and WSI. The FTIR spectrum demonstrated that the chemical groups of the textured samples from WOF and BRF did not change after extrusion. Simultaneous increase of WOF and the die temperature could noticeably change the morphology and crystallinity of the products. The optimized grain-based instant powder in terms of functional properties such as good flowability and lower cohesiveness was determined at WOF 25:75 BRF blend, 170°C, and 27% moisture content. Our results demonstrate an innovative approach for the use of WOF in combination with BRF in the production of nutritious instant powder by extrusion.

研究了饲料水分含量(23% 和 27%)、模头温度(120°C、145°C 和 170°C)、全燕麦粉(WOF)和碎米粉(BRF)的不同比例(25:75、50:50 和 75:25)对挤出物功能和技术特性的影响。结果表明,WOF 含量的增加对 EI、硬度、孔隙率和 WAI 有负面影响,而这一趋势对 WSI 有正面影响。温度越高,WAI 和 WSI 越高,但 EI 和硬度并不随热处理量的变化而变化。增加进料水分含量对提高 BD、硬度和 WAI,降低 EI、孔隙率和 WSI 有显著影响。傅立叶变换红外光谱显示,WOF 和 BRF 制成的纹理样品的化学基团在挤压后没有发生变化。同时提高 WOF 和模具温度会明显改变产品的形态和结晶度。在 WOF 25:75 BRF 混合物、170°C 和 27% 水分含量条件下,确定了功能特性(如良好的流动性和较低的凝聚性)方面的最佳谷物速溶粉。我们的研究结果表明,将 WOF 与 BRF 结合使用以挤压法生产营养速溶粉是一种创新方法。
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引用次数: 0
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Journal of Food Processing and Preservation
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