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The Genetic Interaction on the Quantitative Traits of Prescutellar Bristles in Melon Fly, Bactrccera (Zeugodacus) cucurbitae (Coquillett) 瓜蝇(Zeugodacus) -葫芦科(Coquillett)瓜蝇前刚毛数量性状的遗传互作
Pub Date : 2018-03-31 DOI: 10.6156/JTAR.201803_67(1).0002
E. Cheng, Zu-Hsien Wang, Yu‐Bing Huang, Ming-Yaw Chiang, Hsiu-Ying Lu, Dong-Hong Wu, Chung-Ming Yang, Chun-Chi Nien
前期研究中已針對瓜實蠅Bactrocera cucurbitae (Coquillett)中胸背板之prescutellar bristles (prsc)數目屬數量遺傳(quantitative genetics)機制控制,惟在研究中發現野生型(wild type)之2PHT (2prsc phenotype; 2P-strain)與多剛毛品系(multiple prsc phenotypes; MB-strain)雜交時,F_1子代遵循數量遺傳法則,但F_2之頻度分布(phenotype frequency distribution)中,2PHT卻有異常增加之現象,可能有「非屬數量遺傳」之其他機制介入,因此本研究一方面確認前述觀察之確實性,另一方面則藉此探討其原因。本研究係以2P品系及MB品系分別與MB品系之各表現型(phenotypes)進行兩系列之雜交試驗,並比對F_1與F_2之表現型頻度分布,以分析其異同。結果發現凡以2P品系進行之雜交試驗,F_1均依照數量遺傳法則表現,但F_2均出現2PHT表現型增多之情形,平均幅度為22.5% ± 4%,因此2PHT之增加極有可能來自於專限於2PHT之其他因子表現。以MB品系之12PHT與其他表現型之雜交,無論F_1或F_2則依數量遺傳法則表現,並無任何一種prsc表現型突然增加頻度之現象。研究結果顯示瓜實蠅之中胸背板剛毛數確實依「數量遺傳」之機制控制。但田間族群之表現卻以2PHT占95%之野生型,並未依數量遺傳之連續型(continuous traits)表現,因此,其他遺傳因素之介入,至為明顯。而本研究發現之「2PHT突增」(2PHT surge)現象可能為介入遺傳因素之一,而此現象也的確有提升2PHT野生型表現頻率之效益。
前期研究中已针对瓜实蝇Bactrocera cucurbitae (Coquillett)中胸背板之prescutellar bristles (prsc)数目属数量遗传(quantitative genetics)机制控制,惟在研究中发现野生型(wild type)之2PHT (2prsc phenotype; 2P-strain)与多刚毛品系(multiple prsc phenotypes; MB-strain)杂交时,F_1子代遵循数量遗传法则,但F_2之频度分布(phenotype frequency distribution)中,2PHT却有异常增加之现象,可能有「非属数量遗传」之其他机制介入,因此本研究一方面确认前述观察之确实性,另一方面则借此探讨其原因。本研究系以2P品系及MB品系分别与MB品系之各表现型(phenotypes)进行两系列之杂交试验,并比对F_1与F_2之表现型频度分布,以分析其异同。结果发现凡以2P品系进行之杂交试验,F_1均依照数量遗传法则表现,但F_2均出现2PHT表现型增多之情形,平均幅度为22.5% ± 4%,因此2PHT之增加极有可能来自于专限于2PHT之其他因子表现。以MB品系之12PHT与其他表现型之杂交,无论F_1或F_2则依数量遗传法则表现,并无任何一种prsc表现型突然增加频度之现象。研究结果显示瓜实蝇之中胸背板刚毛数确实依「数量遗传」之机制控制。但田间族群之表现却以2PHT占95%之野生型,并未依数量遗传之连续型(continuous traits)表现,因此,其他遗传因素之介入,至为明显。而本研究发现之「2PHT突增」(2PHT surge)现象可能为介入遗传因素之一,而此现象也的确有提升2PHT野生型表现频率之效益。
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引用次数: 1
Anthracnose of Pitaya (Hylocereus spp.) Caused by Colletotrichum spp., A New Postharvest Disease in Taiwan 台湾采后新病害火龙果炭疽菌引起的炭疽病
Pub Date : 2017-09-01 DOI: 10.6156/JTAR/2017.06603.01
Chu-Ping Lin, P. Ann, Hung Huang, Jay-Ting Chang, J. Tsai, 林筑蘋, 安寶貞, 黃鴻章, 張捷婷, 蔡志濃
紅龍果(白肉Hylocereus undatus,紅肉H. polyrhizus及H. costaricensis)為台灣近20年來廣受栽培的果樹,依2009到2013的田間調查顯示,由Colletotrichum spp.引起的炭疽病為台灣紅龍果主要採收後病害,導致果實倉儲期縮短或降低商品價值,以及經濟價值損失。病斑自果實採收後室溫儲藏5-10 d間始出現,表皮呈現褐色凹陷,嚴重者可能出現水浸狀腐爛。以組織分離罹病果實上的病原菌,並根據形態以及ITS區域序列及類緣分析,至少確定有3種Colletotrichum spp.,包括Colletotrichum gloeosporioides、C. truncatum與C. boninense可感染紅龍果。2009到2013期間自罹染炭疽病之組織上分離各病原之分離率分別為73.4、21.8及4.8%。近年來其他學者發現C. gloeosporioides與C. boninense個別為複合族群(species complex),族群內可再藉由多基因親源分析(multi-locus phylogenetic analysis)區分出多個新種Colletotrichum spp.。為鑑定感染紅龍果之Colletotrichum spp.,未來將再進行多基因親源分析。紅龍果炭疽病為台灣紅龍果重要之採收後病害,本篇在台灣為Colletotrichum spp.引起之紅龍果炭疽病之首篇報導。
红龙果(白肉Hylocereus undatus,红肉H. polyrhizus及H. costaricensis)为台湾近20年来广受栽培的果树,依2009到2013的田间调查显示,由Colletotrichum spp.引起的炭疽病为台湾红龙果主要采收后病害,导致果实仓储期缩短或降低商品价值,以及经济价值损失。病斑自果实采收后室温储藏5-10 d间始出现,表皮呈现褐色凹陷,严重者可能出现水浸状腐烂。以组织分离罹病果实上的病原菌,并根据形态以及ITS区域序列及类缘分析,至少确定有3种Colletotrichum spp.,包括Colletotrichum gloeosporioides、C. truncatum与C. boninense可感染红龙果。2009到2013期间自罹染炭疽病之组织上分离各病原之分离率分别为73.4、21.8及4.8%。近年来其他学者发现C. gloeosporioides与C. boninense个别为复合族群(species complex),族群内可再借由多基因亲源分析(multi-locus phylogenetic analysis)区分出多个新种Colletotrichum spp.。为鉴定感染红龙果之Colletotrichum spp.,未来将再进行多基因亲源分析。红龙果炭疽病为台湾红龙果重要之采收后病害,本篇在台湾为Colletotrichum spp.引起之红龙果炭疽病之首篇报导。
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引用次数: 10
Report on a new invasive species, Pseudococcus dendrobiorum Williams (Hemiptera: Pseudococcidae), in Taiwan. 台湾一新种石斛假球菌(半翅目:假球虫科)报告。
Pub Date : 2015-03-01 DOI: 10.6156/JTAR/2015.06401.01
Shu-Pei Chen, Y. Chiu, Chien-Chung Chen, J. Wong
A new invasive dendrobium mealybug, Pseudococcus dendrobiorum Williams (Hemiptera: Pseudococcidae), through imported orchids (Phalaenopsis spp.) from Vietnam was confirmed by Taiwan Agricultural Research Institute (TARI) in 2013. In this study, morphological characteristics and ribosomal DNA internal transcribed spacer 1 (rDNA-ITS1) sequence were used for the species identification and confirmation of the introduction source. A standard process for elimination and subsequent detection survey of this pest to prevent its spreading was executed by the Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) based on the Plant Protection Act. No individual of this pest was found again during the later surveys. The slide-mounted voucher specimens were deposited in the Insect and Mite Museum, Division of Applied Zoology, TARI.
2013年,台湾农业研究所(TARI)确认了一种通过越南进口兰花(蝴蝶兰属)入侵的石斛属粉蚧新虫——石斛假球菌(Pseudococcus dendrobiorum Williams)。本研究利用形态特征和核糖体DNA内转录间隔1 (rDNA-ITS1)序列进行物种鉴定和引种来源的确认。动植物卫生检验检疫局(BAPHIQ)根据《植物保护法》执行了消除和后续检测有害生物以防止其蔓延的标准程序。在后来的调查中,没有再发现这种害虫的个体。载玻片的代金券标本存放于美国科学院应用动物学部虫螨博物馆。
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引用次数: 0
Revision of the Genus "Sangariola" Jacobson (Coleoptera: Chrysomelidae: Galerucinae: Alticini) “Sangariola”Jacobson属的订正(鞘翅目:金凤蝶科:Galerucinae: Alticini)
Pub Date : 2014-09-01 DOI: 10.6156/JTAR/2014.06303.02
Chi-Feng Lee
Five species of "Sangariola" Jacobson are recognized as valid. "Sangariola hirashimai" Kimoto, 1970, "Sangariola fortune" (Baly 1888), and "Sangariola punctatostriata" (Motschulsky 1861) are redescribed. "Sangariola costata" Chujo, 1935b is removed from synonymy with "S. punctatostriata" (Motschulsky 1861). A new species, "Sangariola yuae" sp. nov., is described from Taiwan. "Charidea nigrosuturalis" var. "obliterate" Pic, 1937 is proposed as a new synonym of S. "fortune" (Baly 1888). Lectotypes are designated for "Charidea fortune" Baly, 1888, "Charidea nigrosuturalis" Pic, 1937, "Galleruca" (sic!) "punctatostriata" Motschulsky, 1861, "Galeruca (Adimonia) multicostata" Jacoby, 1885, and "Sangariola costata" Chujo, 1935b.
五种“Sangariola”Jacobson被认为是有效的。Kimoto, 1970,“Sangariola hirashimai”,“Sangariola fortune”(Baly, 1888)和“Sangariola punctatostriata”(Motschulsky, 1861)被重新描述。“Sangariola costata”Chujo, 1935b从“S”的同义词中删除。斑纹”(莫茨尔斯基1861年)。台湾一新种"Sangariola yuae" sp. nov.。“Charidea nigrosuturalis”var.“obliterate”Pic, 1937被提议作为S.“fortune”的新同义词(Baly 1888)。选型被指定为Baly 1888年的“Charidea fortune”,Pic 1937年的“Charidea nigrosuturalis”,Motschulsky 1861年的“Galleruca”,Jacoby 1885年的“Galeruca (Adimonia) multicostata”和Chujo 1935年的“Sangariola costata”。
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引用次数: 0
A New Species of Anagrus (Hymenoptera: Mymaridae) from Tahiti, with Notes on Egg Parasitoids of Proconiine Sharpshooters (Hemiptera: Cicadellidae: Proconiini) in the World
Pub Date : 2013-12-01 DOI: 10.6156/JTAR/2013.06204.01
S. Triapitsyn
One new fairyfly (Hymenoptera: Mymaridae) species, Anagrus (Anagrus) iti sp. n., described from Tahiti Island, French Polynesia, was reared from eggs of the invasive glassy-winged sharpshooter, Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), which is native to southeastern USA and northeastern Mexico. An overview of the taxonomic and biological studies on the egg parasitoids (Hymenoptera: Aphelinidae, Mymaridae, and Trichogrammatidae) of various proconiine sharpshooters (Cicadellidae: Cicadellinae: Proconiini) in the New World is given. Of particular importance are the natural enemies of H. vitripennis, a notorious vector of the phytopathogenic bacterium, Xylella fastidiosa; with reference to its egg parasitoids and biological control in the non-native range are also provided.
法属波利尼西亚塔希提岛发现的一新种金蝇(膜翅目:金蝇科),是用原产于美国东南部和墨西哥东北部的入侵性玻璃翅神鸟Homalodisca vitripennis(德国)(半翅目:金蝇科)的卵饲养的。本文综述了新大陆不同种类蝉科(蝉科:蝉科:Proconiini)的卵寄生蜂(膜翅目:蚜蜂科、茧蜂科、赤眼蜂科)的分类和生物学研究。特别重要的是玻璃锥虫的天敌,玻璃锥虫是一种臭名昭著的植物致病菌,苛养木杆菌的载体;并对其卵的拟寄生物及在外地的生物防治提供了参考。
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引用次数: 1
Preliminary report on the occurrence of papaya mealybug, Paracoccus marginatus Williams and Granara de Willink, in Taiwan. 台湾番木瓜粉蚧、边缘副绦虫和大粒粒绦虫发生情况的初步报告。
Pub Date : 2011-03-01 DOI: 10.6156/JTAR/2011.06001.07
Chen Shupei, Wong JenYu, Wu WenJen
The papaya mealybug, Paracoccus marginatus Williams and Granara de Willink, is a serious pest of many agricultural plants and ornamentals. It has spread throughout most countries of the Caribbean region, causing huge economic losses to papayas since 1994. This pest alert reports P. marginatus damaging papayas in Taiwan for the first time. Besides it also describes the fundamental biological data of P. marginatus, its existing host plants, and its distribution in Taiwan and around the world.
番木瓜粉蚧(Paracoccus marginatus Williams和Granara de Willink)是许多农业植物和观赏植物的严重害虫。它已经蔓延到加勒比地区的大多数国家,自1994年以来给番木瓜造成了巨大的经济损失。本报告为台湾首次报导边缘小蠹对木瓜的危害。此外,本文还介绍了边际花的基本生物学资料、寄主植物及其在台湾和世界各地的分布。
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引用次数: 15
A Simple and Inexpensive Technique for Estimating Leaf Surface Area of Muskmelon (Cucumis melo L.) 一种简便、廉价的甜瓜叶表面积估算方法
Pub Date : 2010-06-01 DOI: 10.6156/JTAR/2010.05902.01
Huibin Wu, Lit-Fu Chan, M. Wei, Hsiu-Ying Lu
Leaf area is a valuable index in identifying muskmelon (Cucumis melo L.) growth and development. A simple technique was developed for estimating leaf surface area (A) of muskmelon with measurements of leaf length from the sinus base to the apex of leaf along midrib (L) and/or maximum leaf width (W) without the use of any expensive instruments. Leaves for three muskmelon varieties, Chioumih, Zhufen and Tianhun were collected from different levels of the canopy of plants during the growing season in 2007 at Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan. Each leaf was measured for L, W and A. Data analysis were performed by regression technique. Results showed that differences in leaf shape among these varieties affected the performances of prediction accuracy in different models for leaf area estimation. The linear equations having W2 as the independent variable provided the most accurate estimation of A for Chioumih and Zhufen muskmelons, with leaf area coefficients of 0.70 and 0.62, respectively. For Tianhun muskmelon, the equation of A=0.67 × L × W exhibited the highest accuracy of leaf area estimation, and the equations of A=0.62 × L2 and A=0.71 × W2 could also be adopted. With these models, a high degree of 1:1 relationship was obtained between measured and predicted leaf areas, and they produced satisfactory results of lower mean squared deviation (in the range of 451 to 762), lower percentage of deviation for total leaf area (≦1.34%), and lower mean percentage of deviation for individual leaf area (≦0.25%). The linear regression model was simple, rapid, inexpensive and accurate for estimation of leaf surface area of muskmelon and nondestructive to plant growth.
叶面积是判别甜瓜生长发育的重要指标。在不使用任何昂贵仪器的情况下,通过测量从叶窦基部到叶尖沿中脉的叶长(L)和/或最大叶宽(W),建立了一种估算甜瓜叶表面积(A)的简单方法。2007年,在台中五峰台湾农业研究所采集了三种甜瓜品种Chioumih、Zhufen和Tianhun生长季节在植物冠层不同水平的叶片。测定每片叶片的L、W、a含量,采用回归分析方法进行数据分析。结果表明,不同品种叶片形状的差异影响了不同叶面积估算模型的预测精度。以W2为自变量的线性方程能最准确地估计出Chioumih和Zhufen甜瓜的A值,叶面积系数分别为0.70和0.62。天浑甜瓜叶面积估算精度最高的是A=0.67 × L × W,也可采用A=0.62 × L2和A=0.71 × W2的估算公式。利用这些模型,测量叶面积与预测叶面积之间的关系达到了1:1的高度,均方差较小(在451 ~ 762之间),总叶面积的偏差百分比较小(≦1.34%),单个叶面积的平均偏差百分比较小(≦0.25%)。线性回归模型估算甜瓜叶表面积简单、快速、经济、准确,且对植株生长无破坏。
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引用次数: 8
Seasonal Changes of Growth and Leaf Perillaldehyde in Perilla frutescens (L.) Britton 紫苏生长及叶中紫苏醛的季节变化布里顿
Pub Date : 2009-06-01 DOI: 10.6156/JTAR/2009.05802.05
Yuh-Jyuan Lee, Chwen-Ming Yang
Perilla frutescens (L.) Britton, a member of Lamiaceae and a native dicot in Asia, is now distributed worldwide including Taiwan. To further explore its uses as a commercial crop, a comprehensive study regarding the growth behavior and chemical components of this plant species is needed. In the present research, changes in growth traits, including plant height, leaf area index and weights of aerial parts, and leaf perillaldehyde (PA) concentration and content were investigated for plants grown in different growing seasons in the experimental period from 2004 to 2006. The five-leaf stage seedlings were transplanted in March (Season I), April (Season II), May (Season III) June (Season IV) and July (Season V), respectively. Concentration of leaf PA was determined by High Performance Liquid Chromatography (HPLC). Results showed that plant height was taller and leaf area index and fresh weights of aerial parts were larger for plants grown in cooler Season I relative to those plants grown in other warmer seasons (Seasons II-V), implying that warming conditions during growing periods was not in favor to plant growth. Seeds harvested at maturity were found varied in different growing seasons and seed produced in Season V was the lowest. In contrast, the highest value of 500-seed weight also obtained from seeds produced in Seasons V. The PA concentration in the primary leaves on the main stem from position 10 to 15 was higher than others and the quadratic pattern was similar in different growing seasons in 2004–2006. The distribution pattern of leaf PA content was in accordance with leaf PA concentration during the growing periods. In considering the proportions of PA distributed in leaves emerged on the main stem and the lateral branches, the proper time period to harvest the highest quantity of leaf PA for a single plant was from 110 to 120 days after transplanting.
紫苏(L.)布里顿属Lamiaceae,是亚洲的一种本土植物,分布在包括台湾在内的世界各地。为了进一步开发其作为经济作物的用途,需要对其生长行为和化学成分进行全面的研究。本文研究了2004 ~ 2006年不同生长季节生长的紫苏植物株高、地上部分叶面积指数和重量、叶紫苏醛(PA)浓度和含量等生长性状的变化。五叶期移栽时间分别为3月(第1季)、4月(第2季)、5月(第3季)、6月(第4季)和7月(第5季)。采用高效液相色谱法测定叶片中PA的浓度。结果表明:相对于其他暖季(ii ~ v季),冷季I生长的植株株高较高,地上部分叶面积指数和鲜重较大,表明生长期的增温条件不利于植株生长;成熟期收获的种子在不同的生长季节有所不同,第五季产量最低。在2004-2006年不同生长季节,主茎上第10 ~ 15位初生叶的PA浓度均高于其他位置,且其二次型分布规律相似。各生育期叶片PA含量的分布规律与叶片PA浓度一致。考虑到PA在主茎和侧枝上出现的叶片中所占的比例,单株收获叶片PA量最高的适宜时间为移栽后110 ~ 120天。
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引用次数: 3
The Responses of Oncidium Cut Flowers to Ethylene and 1-MCP 石斛切花对乙烯和1-MCP的响应
Pub Date : 2009-03-01 DOI: 10.6156/JTAR/2009.05801.01
Chao-Chia Huang, R. Paull
The ethylene production by Oncidium 'Gower Ramsey' buds and florets and response to 1-methylcyclopropene (1-MCP) were investigated. Small flower buds produced little ethylene (0.12 nL g^(-1) hr^(-1)) and the production declined to being undetectable as the bud grew and became a floret. Ethylene production increased again as the floret began to senesce. A peak of ethylene production occurred (1.5 nL g^(-1) hr^(-1)) at the half senescent floret stage. The rises in ethylene production occurred after the start of floret senescence. Floret buds were more sensitive to exogenous ethylene than the floret, as it took 5 versus 7 days for senescence symptoms to appear at 0.03 nL mL^(-1) exogenous ethylene. The ethylene inhibitor, 1-MCP was effective in prolonging the vase life of both ethylene-treated and non-ethylene-treated cut sprays. When 1-MCP was applied again after 7 days to the same sprays, senescence symptom development was further delayed. Concentration of 1-MCP as low as 235 nL m^(-3) which was one fourth of the concentration recommended for commercial use extended vase life. Bud opening also increased following 1-MCP treatment in ethylene-treated sprays. No difference in response to 1-MCP treatment was found when the treatment was varied from 2 to 12 h and from 10℃ to 28℃. A treatment with 1-MCP was a useful tool to extend vase life of Oncidium cut flower sprays.
研究了蓝心莲(Oncidium 'Gower Ramsey')芽、小花的乙烯产量及其对1-甲基环丙烯(1-MCP)的响应。小花蕾产生的乙烯很少(0.12 nL g^(-1) hr^(-1)),随着花蕾长成小花,乙烯的产量下降到无法检测。当小花开始衰老时,乙烯产量再次增加。半衰老小花期乙烯产量达到峰值(1.5 nL g^(-1) hr^(-1))。乙烯产量的增加发生在小花开始衰老之后。在0.03 nL mL^(-1)外源乙烯条件下,小花芽比小花对乙烯更敏感,衰老症状出现的时间为5天,而不是7天。乙烯抑制剂1-MCP对乙烯处理和非乙烯处理的切割喷雾剂都能有效延长花瓶寿命。7天后再次施用1-MCP时,衰老症状的发展进一步延迟。1-MCP的浓度低至235 nL m^(-3),是商业用途推荐浓度的四分之一,延长了花瓶的使用寿命。在乙烯处理的喷雾剂中,1-MCP处理也增加了芽开度。1-MCP处理时间在2 ~ 12 h、10 ~ 28℃范围内无显著差异。1-MCP处理是延长石斛切花喷雾瓶期的有效手段。
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引用次数: 8
Evaluation of In Vitro Antioxidant and Anti-Lipid Peroxidation Activities of Ching-Pien-Tsao (Pteris multifida Poiret) 青片草(Pteris multifiida Poiret)的体外抗氧化和抗脂质过氧化活性评价
Pub Date : 2009-03-01 DOI: 10.6156/JTAR/2009.05801.06
Tzuching Wang, Hou-I Lee, Chiching Yang
The total antioxidant capacity (TAC), scavenging ability on 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) and superoxide anions, chelating effect on copper ions, and anti FeCl2/H2O2-stimulated linoleic acid peroxidation of aqueous extract of Pteris multifida Poiret (AEPM), were determined in vitro. Results indicated that the AEPM showed a high TAC (86.7% at 20 mg/mL) with ferric thiocyanate method and exhibited the highest Trolox equivalent antioxidant capacity (1.42 mM at 10mg/mL). The scavenging ability on superoxide anion and chelating effect on copper ions of AEPM were 95.4% and 44.7% at 20mg/mL, respectively. The linoleic acid peroxidation inhibition percentage was increased with increasing concentrations and the IC50 was 1000±42μg/mL.
研究了多假羊蹄(Pteris multifiida Poiret, AEPM)水提物的总抗氧化能力(TAC)、对2,2′-氮化杂基(3-乙基苯并噻唑啉-6-磺酸)和超氧阴离子的清除能力、对铜离子的螯合作用以及抗FeCl2/ h2o2刺激的亚油酸过氧化作用。结果表明,采用硫氰酸铁法制备的AEPM在20mg /mL时TAC高达86.7%,在10mg/mL时具有最高的Trolox当量抗氧化能力(1.42 mM)。在20mg/mL浓度下,AEPM对超氧阴离子的清除能力为95.4%,对铜离子的螯合作用为44.7%。亚油酸过氧化抑制率随浓度的增加而增加,IC50为1000±42μg/mL。
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引用次数: 6
期刊
Taiwan Agricultural Research
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