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Development of a Generalizable IRIV-ANN Model for Rapid, Non-Destructive Polysaccharides Detection in Polygonatum Sibiricum 快速、无损检测黄精多糖的广义IRIV-ANN模型的建立
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-27 DOI: 10.1111/jfpe.70354
Bingqian Liu, Anguo Xie, Han Wu, Yunhong Liu

Polysaccharides, as important bioactive components in medicinal and functional food materials, are key indicators for quality evaluation. Conventional analytical methods for polysaccharides quantification often require laborious sample preparation and may cause irreversible damage to samples, which limits their suitability for routine quality control. To overcome these limitations, we developed a rapid and non-destructive detection approach by integrating hyperspectral imaging with an iteratively retained information variables-artificial neural network (IRIV-ANN) model. Spectral data (400–1000 nm) were processed using the IRIV algorithm, which classified variables into four categories-strong information, weak information, no information, and interference. The optimal subset was then used to construct an ANN model, and its performance was compared with models based on shuffled frog leaping algorithm (SFLA), successive projections algorithm (SPA), one-dimension convolutional neural network (1D-CNN), full-spectrum ANN, and multiple linear regression (MLR). The IRIV-ANN model successfully eliminated redundant variables, reduced model complexity, and improved predictive performance, achieving a coefficient of determination (R2) of 0.9301 and a root mean square error (RMSE) of 0.7348 for the prediction set. The characteristic wavelengths selected were closely associated with vibrational changes in O-H and C-H functional groups, providing chemical interpretability of the spectral features. This method provides an efficient and reliable analytical tool for rapid quantification of polysaccharides and has considerable potential for broader application in the quality control of functional foods, traditional medicines, and other plant-derived materials.

多糖作为药用和功能性食品原料中的重要生物活性成分,是质量评价的关键指标。传统的多糖定量分析方法通常需要费力的样品制备,并可能对样品造成不可逆的损害,这限制了它们对常规质量控制的适用性。为了克服这些限制,我们通过将高光谱成像与迭代保留信息变量人工神经网络(IRIV-ANN)模型相结合,开发了一种快速、无损的检测方法。光谱数据(400-1000 nm)采用IRIV算法进行处理,该算法将变量分为强信息、弱信息、无信息和干扰四类。然后利用最优子集构建人工神经网络模型,并与基于shuffle frog跳跃算法(SFLA)、逐次投影算法(SPA)、一维卷积神经网络(1D-CNN)、全谱人工神经网络和多元线性回归(MLR)的模型进行性能比较。IRIV-ANN模型成功地消除了冗余变量,降低了模型复杂性,提高了预测性能,预测集的决定系数(R2)为0.9301,均方根误差(RMSE)为0.7348。所选择的特征波长与O-H和C-H官能团的振动变化密切相关,提供了光谱特征的化学可解释性。该方法为多糖的快速定量分析提供了一种高效可靠的分析工具,在功能食品、传统药物和其他植物源性材料的质量控制中具有相当大的应用潜力。
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引用次数: 0
Quantitative Evaluation of Value Engineering Metrics for Productivity Improvement Through Function and Cost Analysis in Small-Scale Cooling Units 通过功能和成本分析提高小型冷却机组生产率的价值工程度量的定量评价
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-27 DOI: 10.1111/jfpe.70357
Sachin Mohite, Prashant Patane, Ashish Pawar

The fundamentals of value engineering are presented in this article and can be applied to any small-scale refrigeration water cooler manufacturing industry. Value engineering is a clever technical engineering approach that is also a cost-effective way to get the required product function at the lowest possible cost. A case study of a refrigeration water cooling system is conducted, where the value engineering method is used to modify the component design and material. According to the case study, the usage of different hardware components, expensive materials, excessive material size, etc., raises the cost needlessly. The compressor, condenser, condenser fan, evaporator, refrigeration control system, outer body, taps, drain tray, and other components of the water cooler have been chosen, and value engineering is applied to these components to cut costs. The functional coefficient, cost coefficient, and value coefficient are calculated to analyze the correlation between various functions and their associated cost. Through the use of value engineering techniques in refrigeration water cooling systems, cost savings are accomplished.

本文介绍了价值工程的基本原理,并可应用于任何小型制冷水冷机制造行业。价值工程是一种聪明的技术工程方法,也是一种以尽可能低的成本获得所需产品功能的经济有效的方法。以某制冷水冷却系统为例,采用价值工程方法对其部件设计和材料进行修改。根据案例研究,不同硬件部件的使用,昂贵的材料,过大的材料尺寸等,增加了不必要的成本。对水冷机的压缩机、冷凝器、冷凝器风机、蒸发器、制冷控制系统、外体、水龙头、排水盘等部件进行了选型,并对这些部件进行了价值工程的应用,以降低成本。通过计算功能系数、成本系数和价值系数来分析各种功能及其相关成本之间的相关性。通过在制冷水冷却系统中使用价值工程技术,实现了成本节约。
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引用次数: 0
Analyzing Food Systems to Strengthen Food Security Towards Achieving Sustainable Development Goal 2 (Zero Hunger) and Goal 12 (Responsible Consumption and Production) in Mexico: A Comparison Between Lettuce Growth in Vertical Farms and Outdoor Production Systems 分析粮食系统以加强粮食安全,实现墨西哥可持续发展目标2(零饥饿)和目标12(负责任的消费和生产):垂直农场和户外生产系统生菜生长的比较
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-27 DOI: 10.1111/jfpe.70312
Mayeli Peralta-Contreras, Linda Carolina Hernandez-Lozano, George De Feis, Julieta Domínguez-Soberanes

This study investigates the impact of production systems, including: (1) open production system (OP) and (2) vertical farm (VF) on lettuce quality. The study aligns with SDG 2 (Zero Hunger) and SDG 12 (Responsible Consumption and Production). Germination experiments were carried out inside a climate-controlled farm for 4 weeks with two varieties of lettuces: Ruby Sky Lettuce (LR) and Patrona Lettuce (LO). Then, seedlings were planted in two experimental settings: an open production system (OP) with a dripping irrigation system and a vertical farm treatment (VF) with an aero hydroponic irrigation system arrangement with a 200 L nutrient tank. A comparison test was carried out between Patrona Lettuce (LO) and Ruby Sky Lettuce (LR), which were harvested in two different production systems. The two lettuce varieties, Ruby Lettuce (RL) and Patrona Lettuce (PL), were sampled for microbiological analysis and counts using the following quality indicators: aerobic mesophilic microorganisms, coliforms, fungi, and yeast to compare the production systems. Microbial analysis revealed significantly lower counts of aerobic mesophilic microorganisms, coliforms, and fungi in vertical lettuce. For Ruby Sky, outdoor production resulted in 29-, 32-, and 37-times higher counts, respectively. Patrona exhibited similar trends. These findings suggest that vertical farming enhances food safety by minimizing microbial contamination. VF can empower small-scale farmers, improve agricultural productivity, and enhance food security, particularly in regions facing limited land and water resources, thus reaching towards SDG 2 and SDG 12.

本研究探讨了生产系统对生菜品质的影响,包括:(1)开放式生产系统(OP)和(2)垂直农场(VF)。该研究符合可持续发展目标2(零饥饿)和可持续发展目标12(负责任的消费和生产)。在一个气候控制的农场内进行了为期4周的发芽试验,试验材料为红宝石天莴苣(LR)和Patrona Lettuce (LO)两种生菜。然后,将幼苗种植在两个试验环境中:开放式生产系统(OP)与滴灌系统,垂直农场处理(VF)与空气水培灌溉系统布置,200 L营养池。对两种不同生产制度下收获的白顶莴苣(LO)和红宝石天莴苣(LR)进行了比较试验。采用好氧中温微生物、大肠菌群、真菌和酵母等品质指标,对红宝石莴苣(RL)和Patrona莴苣(PL)这两个生菜品种进行微生物学分析和计数,比较两种生产体系。微生物分析显示,垂直生菜的好氧中温微生物、大肠菌群和真菌数量显著降低。对于《Ruby Sky》,户外制作的结果分别是前者的29倍、32倍和37倍。Patrona也表现出类似的趋势。这些发现表明,垂直农业通过最大限度地减少微生物污染来提高食品安全。VF可以增强小农的权能,提高农业生产力,加强粮食安全,特别是在土地和水资源有限的地区,从而实现可持续发展目标2和12。
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引用次数: 0
Evaluation of Drying Kinetics and Quality Assessment of Desiccated Coconut Produced by Conventional Hot-Air Drying Method 传统热风干燥法生产的椰子干燥动力学评价及质量评价
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-22 DOI: 10.1111/jfpe.70349
Panoth Abhirami, Taruna Varghese, C. K. Sunil, Venkatachalapathy Natarajan

This study examined the drying kinetics and quality of desiccated coconut through hot-air drying at varying temperatures of 80°C, 85°C, and 90°C, reducing moisture content from 48% ± 2% (w.b.) to 2% ± 1.1% (w.b.). Several physicochemical parameters were assessed, including color, water activity, energy consumption, oil characteristics, surface morphology, functional groups, and fatty acid profiles. The findings revealed that drying temperature significantly affected the color attributes of the coconut, primarily driven by the Maillard reaction and sugar concentration, with desiccated coconut at 85°C demonstrating optimal color properties. Water activity consistently decreased with increasing drying temperature. Higher drying temperatures resulted in a notable reduction of free fatty acids (FFAs); however, the peroxide value of the extracted oil displayed an inverse correlation. Microstructural observations indicated that extended drying resulted in pore formation due to moisture evaporation, with 90°C exhibiting an oily surface indicative of oil cell breakdown absent at 85°C. Additionally, lauric acid content increased with drying temperature. FTIR analysis revealed distinct O–H stretching vibrations, alongside C–H and C═O stretching bonds, confirming the presence of carbohydrates and ester groups in coconut fat. Thus, optimizing the drying process at 85°C is recommended for improving the quality of desiccated coconut, suitable for industrial applications.

本研究通过在80°C、85°C和90°C的不同温度下热风干燥,研究了干燥动力学和干燥质量,将水分含量从48%±2% (w.b.)降低到2%±1.1% (w.b.)。评估了几种物理化学参数,包括颜色、水活度、能量消耗、油特性、表面形态、官能团和脂肪酸分布。研究结果表明,干燥温度对椰子的颜色属性有显著影响,主要受美拉德反应和糖浓度的影响,干燥后的椰子在85°C时表现出最佳的颜色属性。随着干燥温度的升高,水分活度持续下降。较高的干燥温度导致游离脂肪酸(FFAs)显著减少;然而,提取油的过氧化值呈负相关。微观结构观察表明,由于水分蒸发,延长的干燥导致孔隙形成,在90°C时表现出油状表面,表明在85°C时没有油细胞破裂。月桂酸含量随干燥温度的升高而升高。FTIR分析显示明显的O - h拉伸振动,以及C - h和C = O拉伸键,证实了椰子脂肪中碳水化合物和酯基团的存在。因此,建议在85°C下优化干燥工艺,以提高干燥椰子的质量,适合工业应用。
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引用次数: 0
Prediction of the Key Rheological Characteristics of Wheat Dough Based on the Spatial Deformation Difference Before and After the Bursting of the Dough 基于爆裂前后空间变形差的小麦面团关键流变特性预测
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-22 DOI: 10.1111/jfpe.70255
Xiuzhi Luo, Shihui Tan, Xin Shi, Jianfei Xing, Xiuying Tang

Dough toughness and extensibility are key rheological properties directly linked to the quality of dough-based products. Thus, this research employed a custom-built device to inflate dough sheets into bubbles, with a 3D imaging system recording real-time point cloud data of these formations. The investigation specifically targeted point cloud imagery capturing both the frame displaying maximum spatial deformation height in dough bubbles and its subsequent frame. Four novel computational methods were developed to quantify four distinct deformation characteristics from the selected two-frame bubble point cloud images. These parameters were subsequently fed into three machine learning architectures for autonomous prediction of dough toughness and extensibility. Experimental findings revealed optimal detection accuracy when utilizing deformation feature n's immediate recovery index (En) with the ELM algorithm, attaining Rp coefficients of 0.882 and0.940 for extensibility and toughness assessments, respectively.

面团的韧性和延展性是直接关系到面团产品质量的关键流变学特性。因此,本研究采用了一种定制的设备将面团片充气成气泡,并使用3D成像系统记录这些地层的实时点云数据。该研究特别针对点云图像,捕获了面团气泡中显示最大空间变形高度的帧及其后续帧。开发了四种新的计算方法来量化所选的两帧气泡点云图像的四种不同的变形特征。这些参数随后被输入到三个机器学习架构中,用于自主预测面团的韧性和延展性。实验结果表明,利用变形特征n的即时恢复指数(En)与ELM算法的检测精度最佳,扩展和韧性评估的Rp系数分别为0.882和0.940。
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引用次数: 0
Preparation of Platinum Nanowire Supported on Carbon Catalyst and Its Effect on the Electrochemical Hydrogenation of Soybean Oil 碳催化剂负载铂纳米线的制备及其对大豆油电化学加氢的影响
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-21 DOI: 10.1111/jfpe.70302
Huanyu Zheng, Jingqi Cui, Hui Jia, Siqi Wang, Xinghui Wu, Dongling Yan, Yunxin Ying, Dan Li

This study aims to develop a novel oil hydrogenation process using a solid polymer electrolyte (SPE) electrochemical reactor, focusing on optimizing the preparation of platinum nanowire (Pt-NWs) catalysts and their application in hydrogenation. By controlling the concentration of the reducing agent (a formic acid-to-water ratio of 7:600) and maintaining the reaction temperature at 20°C, a high-performance Pt-NWs/C catalyst was synthesized. Since the <111> crystal plane is considered the most active in the Pt catalyst, characterization showed that the Pt-NWs/C exhibited sharper x-ray diffraction peaks in that direction compared to commercial Pt/C, along with a more hydrophobic catalytic layer (contact angle of 135° ± 0.3°). In addition, the Pt-NWs catalyst also demonstrates superior performance in electrochemical stability (a 29.31% decrease). The catalyst was applied in an SPE hydrogenation reactor, and its performance in soybean oil hydrogenation was systematically evaluated. The results show that the hydrogenated oil containing Pt-NWs/C has good selectivity, while transfatty acid (TFA) content was about 13% lower than with commercial Pt/C. Electronic nose analysis confirmed that the hydrogenated oil retained favorable flavor characteristics. Polarized light microscopy showed that oil produced by Pt-NWs/C had a finer (approximately 20 nm) and more uniform crystal structure. The Pt-NWs/C catalyst exhibited excellent catalytic activity (ECSA value of 27.40 m2 g−1) and selectivity by the SPE reactor, offering a promising technological pathway for producing healthy hydrogenated oil with low TFA content.

本研究旨在开发一种基于固体聚合物电解质(SPE)电化学反应器的石油加氢新工艺,重点优化铂纳米线(Pt-NWs)催化剂的制备及其在加氢中的应用。通过控制还原剂浓度(甲酸水比为7:60)和反应温度保持在20℃,合成了高性能Pt-NWs/C催化剂。由于<;111>;晶面被认为是Pt催化剂中最活跃的,表征表明,与商用Pt/C相比,Pt- nws /C在该方向上表现出更清晰的x射线衍射峰,并且具有更疏水的催化层(接触角为135°±0.3°)。此外,Pt-NWs催化剂在电化学稳定性方面也表现出优异的性能(降低了29.31%)。将该催化剂应用于SPE加氢反应器,并对其在大豆油加氢中的性能进行了系统评价。结果表明,含Pt- nws /C的加氢油具有良好的选择性,反式脂肪酸(TFA)含量比含Pt/C的加氢油低13%左右。电子鼻分析证实,氢化油保留了良好的风味特征。偏振光显微镜观察表明,Pt-NWs/C制备的油具有更细(约20 nm)和更均匀的晶体结构。Pt-NWs/C催化剂具有良好的催化活性(ECSA值为27.40 m2 g−1)和选择性,为制备低TFA含量的健康氢化油提供了一条有前景的技术途径。
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引用次数: 0
Chitosan-Based Nanocomposite Films Loaded With Composite Essential Oil Nanoemulsions for Pork Preservation 负载复合精油纳米乳剂的壳聚糖基纳米复合膜用于猪肉保鲜
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-21 DOI: 10.1111/jfpe.70336
Jing Li, Kang Yang, Xiaoqin Shi, Tao Li, Lang Guo, Yong Guo, Xinhui Wang

In this study, carvacrol (CA)/thymol (TH) nanoemulsions (CT NEs) were first prepared by the ultrasonic emulsification method, and then the effects of various nanoemulsion concentrations on the physicochemical properties and biological activities of chitosan/pullulan (CS/PU) nanocomposite films were systematically evaluated. The CT NEs exhibited good stability with a high encapsulation efficiency of 90.29% ± 0.95%, and they were well integrated in the CS/PU matrix as confirmed by the microstructure characterization. The performances of the nanocomposite films were significantly enhanced by CT NEs, including the improvements of mechanical properties and barrier properties. The CS/PU-CT films exerted notable antioxidant and antibacterial activities. In the end, the CS/PU-3CT film (with 3.0% w/v CA and TH), with the best antioxidant and antibacterial performance, was applied to the preservation of pork, and its shelf life was extended for 10 days compared to the CK group. The film loaded with 3CT NEs exhibits outstanding performance and offers significant prospects for use in food packaging.

本研究首先采用超声乳化法制备了香芹酚(CA)/百里香酚(TH)纳米乳液(CT NEs),然后系统地考察了不同纳米乳液浓度对壳聚糖/普鲁兰(CS/PU)纳米复合膜理化性能和生物活性的影响。CT - NEs具有良好的稳定性,包封效率为90.29%±0.95%,并且在CS/PU基体中具有良好的集成性能。纳米复合膜的力学性能和势垒性能得到了显著提高。CS/PU-CT膜具有显著的抗氧化和抗菌活性。最后,将含有3.0% CA和TH的CS/PU-3CT薄膜用于猪肉的保鲜,其抗氧化和抗菌性能最好,比CK组延长了10 d。负载3CT NEs的薄膜表现出优异的性能,在食品包装中具有重要的应用前景。
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引用次数: 0
Engineering Surface Defect Chemistry of Cerium Oxide Nanoparticles to Enhance the Stability of Nano-Enriched Edible Oils Systems and Emulsions 氧化铈纳米颗粒的工程表面缺陷化学以提高纳米强化食用油体系和乳剂的稳定性
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-21 DOI: 10.1111/jfpe.70344
Sreemoyee Chakraborty, Debabrata Bera, Lakshmishri Roy, Chandan Kumar Ghosh

Engineering oxidative and structural stability in oil-enriched food systems remains a primary focus in food process engineering, particularly in polyunsaturated fatty acid (PUFA)-enriched food systems. The oxygen-vacancy-enriched cerium oxide nanoparticles (CeO2 NPs) were synthesized by a controlled synthesis route optimizing the surface defect chemistry and antioxidant capability in the present study. Structural characteristics, including Ce3+/Ce4+ ratio and oxygen vacancy concentration, were established by XRD, XPS, DLS, and UV–Vis methods. Prepared nanoparticles were incorporated into edible oils (rice bran and sunflower), and storage challenges in a high-oil emulsion-based mayonnaise were performed in an effort to explore designed nanoparticles' function in stabilizing Pickering-type food systems. Oxidative stability was ascertained by peroxide and anisidine value, and characterization of emulsion by textural, interfacial, and sensory tests. Addition of the CeO2 NPs into the edible oils significantly improved oxidative stability, as evidenced by lower peroxide and p-anisidine values after prolonged storage. The emulsions at final stage also showed improved interfacial stability, greater texture profile, and higher sensory acceptance during storage at low temperature, with retained resistance against oxidative as well as structural deterioration. Most importantly, traditional workflows on production of mayonnaise were perfectly compatible with the designed nano-process engineering methodology, involving no fundamental changes in formulations. These findings lay the foundation for oxygen-vacancy-engineered CeO2 NPs as multifunctional process additives in the creation of next-generation emulsions with extended shelf life, improved functional efficacy, and clean label characterization. The research advances progress in the field of nano-enabled food process engineering and systematic characterization of structure–function–process relationships.

富油食品系统的氧化和结构稳定性工程仍然是食品加工工程的主要焦点,特别是在富含多不饱和脂肪酸(PUFA)的食品系统中。采用可控的合成路线合成了富氧氧化铈纳米颗粒(CeO2 NPs),优化了其表面缺陷化学性质和抗氧化性能。通过XRD、XPS、DLS和UV-Vis等方法确定了材料的结构特征,包括Ce3+/Ce4+比和氧空位浓度。将制备的纳米颗粒掺入食用油(米糠和向日葵)中,并在高油乳化蛋黄酱中进行储存挑战,以探索设计的纳米颗粒在稳定pickering型食品体系中的功能。氧化稳定性通过过氧化物和茴香胺值来确定,并通过结构、界面和感官测试来表征乳液。在食用油中添加CeO2 NPs显著提高了氧化稳定性,证明了在长时间储存后过氧化氢和对茴香胺值降低。在低温贮藏过程中,乳状液的界面稳定性、结构轮廓和感官接受度均有所提高,同时具有抗氧化性和结构劣化性。最重要的是,蛋黄酱生产的传统工作流程与设计的纳米工艺工程方法完全兼容,配方没有根本变化。这些发现为氧空位工程CeO2 NPs作为多功能工艺添加剂,用于制造具有延长保质期、提高功能功效和清洁标签表征的下一代乳剂奠定了基础。该研究推动了纳米食品加工工程和结构-功能-过程关系系统表征领域的进展。
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引用次数: 0
Online Intelligent Quality-Efficiency Detection Method for Buckwheat Hulling Based on an Enhanced YOLOv8 Algorithm 基于改进YOLOv8算法的荞麦去皮品质效率在线智能检测方法
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-21 DOI: 10.1111/jfpe.70347
Bin Fan, Man Xie, Jingfu Dong, Zheying Zong, Tianshun Jin, Xiangyu Gao, Wenliang Du

Accurate detection of hulling quality-efficiency is critical for optimizing buckwheat processing and product quality. Current reliance on manual visual inspection is inefficient and impedes industrial progress. To address this issue, an online intelligent detection system is proposed based on an improved YOLOv8 algorithm. The model incorporates three key modifications: (1) a Dual Attention mechanism within the C2f module; (2) the WIoUv3 loss function replacing CIoU; and (3) an expansion to four detection heads. This refined framework enables real-time detection of buckwheat hulling efficiency. The system consists of four core components: an image-based detection module, a 6QB-150 buckwheat huller, control actuators, and auxiliary subsystems. An image acquisition device captures real-time samples, and the improved model accurately distinguishes three hulling statuses: hulled kernels, broken kernels, and unhulled kernels. Detection results are transmitted to a host computer, which interfaces with a PLC to regulate a three-phase asynchronous motor, enabling adaptive control of grinding disc speed and equipment operation. Ablation experiments demonstrated substantial performance improvements over the baseline, with increases of 7.7% in precision, 8.1% in recall, 5.9% in mAP@50, and 4.2% in mAP@50–95. Comparative trials confirmed the model's superiority over YOLOv5, YOLOv6, YOLOv8n, and YOLOv10n, with negligible computational increase. In practical experiment, model-detected rates for broken, hulled, and unhulled kernels deviated by merely +0.13%, −0.71%, and +0.53%, respectively, from manual counts, attesting to its reliability. The deployment of this system significantly enhances operational efficiency, offering an effective solution for automated quality control in buckwheat processing and contributing to agricultural modernization.

脱壳质量效率的准确检测是优化荞麦加工和提高产品质量的关键。目前对人工目视检查的依赖效率低下,阻碍了工业进步。针对这一问题,提出了一种基于改进YOLOv8算法的在线智能检测系统。该模型包含三个关键修改:(1)C2f模块内的双重注意机制;(2) WIoUv3损失函数代替CIoU;(3)扩展到四个检测头。这种改进的框架能够实时检测荞麦脱壳效率。该系统由四个核心组件组成:基于图像的检测模块、6QB-150荞麦脱壳机、控制执行器和辅助子系统。图像采集装置实时采集样本,改进后的模型能准确区分去壳、碎壳和未去壳三种剥壳状态。检测结果传输到上位机,上位机与PLC接口,调节三相异步电动机,实现对砂轮转速和设备运行的自适应控制。消融实验表明,与基线相比,性能有了实质性的改善,精确度提高了7.7%,召回率提高了8.1%,mAP@50提高了5.9%,mAP@50 -95提高了4.2%。对比试验证实了该模型优于YOLOv5、YOLOv6、YOLOv8n和YOLOv10n,计算量的增加可以忽略不计。在实际实验中,模型对破碎、去壳和未去壳籽粒的检测率与人工计数的偏差分别仅为+0.13%、−0.71%和+0.53%,证明了模型的可靠性。该系统的部署大大提高了操作效率,为荞麦加工的自动化质量控制提供了有效的解决方案,为农业现代化做出了贡献。
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引用次数: 0
Real-Time PCR Detection of Adulteration of Olive Oil With Soybean Oil Based on Optimized CTAB DNA Extraction Method 基于优化CTAB DNA提取法的大豆油掺假橄榄油实时荧光定量PCR检测
IF 2.9 3区 农林科学 Q3 ENGINEERING, CHEMICAL Pub Date : 2026-01-21 DOI: 10.1111/jfpe.70351
Kai Chen, JianZhi Luo, Bin Xiao, XueWei Yuan, Juan Zhang, Nan Wang, LiQiang Liu, AiKe Li, Ailiang Chen

This study established an optimized CTAB-based DNA extraction method for edible oils, which enables stable and reliable DNA recovery from commercially available edible oils for authenticity identification and adulteration detection. Real-time PCR was used to assess DNA precipitation time and organic solvent extraction, revealing that longer precipitation increased inhibitors, hindering PCR amplification, while organic solvents enhanced efficiency. The method was applied to detect adulteration in commercially available soybean and olive oils. A standard curve based on real-time PCR Ct values demonstrated the ability to detect as low as 10% soybean oil in olive oil, with recovery rates of 92% and 80% for samples spiked with 70% and 30% soybean oil, respectively. The coefficients of variation (CV) were 11% and 14%, within acceptable ranges. This reliable method provides a valuable tool for edible oil authentication and adulteration detection.

本研究建立了一种优化的基于ctab的食用油DNA提取方法,可以稳定可靠地从市售食用油中提取DNA,用于真伪鉴定和掺假检测。利用Real-time PCR对DNA的沉淀时间和有机溶剂萃取进行评价,发现较长的沉淀时间增加了抑制剂,阻碍了PCR扩增,而有机溶剂提高了效率。该方法应用于市售大豆油和橄榄油的掺假检测。基于实时PCR Ct值的标准曲线显示,该方法能够检测橄榄油中低至10%的大豆油,对于添加70%和30%大豆油的样品,回收率分别为92%和80%。变异系数(CV)分别为11%和14%,在可接受范围内。该方法可靠,为食用油的鉴别和掺假检测提供了有价值的工具。
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引用次数: 0
期刊
Journal of Food Process Engineering
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