Journal of immunological methods最新文献

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Evaluation of the i-Tracker infliximab and i-Tracker anti-infliximab total antibodies ECLIA on the random-access automated analyzer IDS-iSYS for the routine clinical monitoring of infliximab-treated patients 在IDS-iSYS随机存取自动分析仪上评价i-Tracker英夫利昔单抗和i-Tracker抗英夫利昔单抗总抗体ECLIA对英夫利昔单抗治疗患者的常规临床监测

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113933

Akpedje S. Dossou, Serena Kang, Tahira Kalhoro, Eduardo Castro Echeverry, Nathan C. Horton

引用次数: 0

Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis 标准抗磷脂抗体的持久性与滴度、同型和抗磷脂综合征诊断的关系

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113932

Arevik Ghazaryan , Mouhamed Yazan Abou-Ismail , Abdulrahman Saadalla

引用次数: 0

Development of a sensitive sandwich ELISA for the envelope domain III protein of dengue virus type 2 登革病毒2型包膜结构域III蛋白灵敏夹心ELISA试剂盒的建立。

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.114000

Ziting Ma , Jingyi Su , Zelin Han , Ximing Ren , Jinnian Guo , Lu Jiang , Suqing Zhao

Dengue, caused by the dengue virus (DENV), is a rapidly spreading infectious disease that poses a significant global health challenge. Rapid and accurate diagnosis is critical for effective disease management and epidemic control, as dengue is a kind of vector-borne disease and lacks definitive medical treatments. Current antibodies and antigens for DENV immunoassay, including immunoglobulin G (IgG), immunoglobulin M (IgM), and non-structural protein 1 (NS1), have drawbacks like cross-reaction and false-negative results in the late stages of primary infection or secondary infection. Envelope domain III (EDIII) protein, which is immunogenic and is a recognition region and binding site for DENV receptors, can also be the potential detection target of DENV infection. However, few studies of EDIII protein as a detection target have been reported. Therefore, this study developed a sensitive and specific ELISA for DENV-2_EDIII protein detection. Specific polyclonal antibodies (pAbs) against the DENV-2_EDIII protein were generated by immunizing the New Zealand White rabbits with the recombinant DENV-2_EDIII protein. Using these pAbs, a highly sensitive and specific ELISA method was developed. The assay demonstrated a wide detection range (7.8 ng/mL to 500 ng/mL) and a low limit of detection (LOD) of 0.156 ng/mL and showed no cross-reactivity with other orthoflaviviruses. These findings may provide a new methodological framework and research direction for the diagnosis of dengue after further clinical verifications.

登革热由登革热病毒（DENV）引起，是一种迅速蔓延的传染病，对全球卫生构成重大挑战。快速和准确的诊断对于有效的疾病管理和流行病控制至关重要，因为登革热是一种媒介传播的疾病，缺乏明确的医学治疗。目前用于DENV免疫测定的抗体和抗原，包括免疫球蛋白G （IgG）、免疫球蛋白M （IgM）和非结构蛋白1 (NS1)，在原发性感染或继发性感染的晚期存在交叉反应和假阴性结果等缺点。包膜结构域III （EDIII）蛋白具有免疫原性，是DENV受体的识别区和结合位点，也可能是DENV感染的潜在检测靶点。然而，将EDIII蛋白作为检测靶点的研究报道较少。因此，本研究建立了一种灵敏、特异的DENV-2_EDIII蛋白ELISA检测方法。用重组DENV-2_EDIII蛋白免疫新西兰大白兔，获得了DENV-2_EDIII蛋白的特异性多克隆抗体（pAbs）。利用这些pAbs，建立了一种高灵敏度和特异性的ELISA方法。该方法检测范围广（7.8 ng/mL ~ 500 ng/mL），低检出限（LOD）为0.156 ng/mL，与其他正黄病毒无交叉反应性。这些发现可能为进一步临床验证登革热的诊断提供新的方法学框架和研究方向。

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引用次数: 0

Evaluation of anti-nuclear antibody (ANA) patterns and its associations with autoimmune disease biomarkers 评估抗核抗体（ANA）模式及其与自身免疫性疾病生物标志物的关联

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113924

Vincent Ricchiuti , Min Kyung Lee , David Alfego , Stanley J. Naides , Ajay Grover

引用次数: 0

Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections 同时检测马雅罗病毒和基孔肯雅病毒IgM和IgG可改善急性马雅罗病毒感染的诊断

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113939

Maite Sabalza , Julia Klemens , Marleen Janku , Erik Lattwein , Anette Schulz , Oliver Klemens

引用次数: 0

Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring 用于诊断和治疗监测的血管内皮生长因子- d (VEGF-D) ELISA的性能特征

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113964

Lorraine Abushanab, Maria Meriwether, Yongbao Wang, Claire Coeshott

引用次数: 0

Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity 乳糜泻风险分层：HLA基因型与乳糜泻特异性抗体阳性的相关性

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113949

Tracie Profaizer , Ann Pole , Michael J. Thompson , Eszter Lazar-Molnar

引用次数: 0

Improved accessibility of the high precision T-SPOT®.TB test for laboratories and patients through the automation of cell isolation 提高了高精度T-SPOT®的可及性。结核病检测可通过实验室和患者的细胞分离自动化进行

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113934

Thomas Jackson-Soutter , Shu-Hua Wang , Andre Trollip , Ruth Brignall , Maite Sabalza , Mathew Quinn , Blanca Restrepo , Mathew Dudek

引用次数: 0

Variation in DNA enrichment among deuterium labeling studies is largely explained by different background corrections 在氘标记研究中DNA富集的差异很大程度上是由不同的背景校正来解释的。

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113987

Erdem Şanal , José A.M. Borghans , Rob J. de Boer

Heavy water (

D_{2} O

) labeling can be used to track the dynamics of circulating cells in vivo.

D_{2} O

is administered via the drinking water, dividing cells become labeled by incorporating deuterium in their newly formed DNA, and the accrual of labeled deoxyribose molecules in the DNA is measured by gas-chromatography mass-spectrometry (GC–MS). Deuterium labeling studies need to correct for a background enrichment that is due to several naturally occurring isotopes, but the procedure for performing this correction differs between studies. We show that a background correction that is based upon subtracting the background enriched fraction (i.e., the atom percent excess, APE) underestimates the true enrichment, whereas one that is based upon subtracting the background enriched ratio (i.e., the tracer-to-tracee ratio, TTR) provides an enrichment that correctly describes the additional effect of the deuterium labeling. This difference is reflected in the so-called ‘amplification factor’, which quantifies the relative enrichment of deoxyribose in a fast reference population of cells to the enrichment in the body water. Using mechanistic binomials we reanalyze deuterium labeling data and show that previous studies based upon an APE-based background correction have underestimated this amplification factor by about 25%, which explains most of the variation among studies in their average amplification factor. We propose a novel model in which the amplification factor is defined by a binomial expression allowing for metabolic differences between volunteers in the contribution of body water to the hydrogens incorporated into deoxyribose during the synthesis of DNA.

重水（D2O）标记可用于跟踪体内循环细胞的动力学。通过饮用水给予D2O，分裂细胞通过将氘加入其新形成的DNA中进行标记，并通过气相色谱-质谱（GC-MS）测量DNA中标记的脱氧核糖分子的累积。氘标记研究需要校正由几种天然同位素引起的背景富集，但在不同的研究中进行校正的程序不同。我们表明，基于减去背景富集分数（即原子过量百分比，APE）的背景校正低估了真正的富集，而基于减去背景富集比（即示踪物与示踪物之比，TTR）的背景校正提供了正确描述氘标记附加效应的富集。这种差异反映在所谓的“放大因子”上，该因子将快速参考细胞群中脱氧核糖的相对富集量与体内水体中的富集量进行量化。使用机制二项法，我们重新分析了氘标记数据，并表明先前基于ape背景校正的研究低估了该放大因子约25%，这解释了大多数研究之间平均放大因子的差异。我们提出了一种新的模型，其中放大因子由二项表达式定义，允许志愿者之间的代谢差异，在DNA合成过程中，身体水对氢结合到脱氧核糖中的贡献。

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引用次数: 0

Single-tiered serological diagnostic test for Lyme disease 莱姆病单层血清学诊断试验

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods

Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113941

Kirsten Fagan , Marta Urban , Paul Rocco LaSala , Charles Caso , James Needham , Anu Singh Maharjan

引用次数: 0

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