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Journal of immunological methods最新文献

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Evolution and characterization of β-Lactam allergy profiles and stability of sIgE over two decades in adult patients 20年来成人患者β-内酰胺过敏谱的演变和特征以及sIgE的稳定性
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113959
Jessica Murphy , Jose-Julio Laguna , Merce Tena , Cosmin Boteanu , Maria-Luisa Sanchez-Millan
{"title":"Evolution and characterization of β-Lactam allergy profiles and stability of sIgE over two decades in adult patients","authors":"Jessica Murphy , Jose-Julio Laguna , Merce Tena , Cosmin Boteanu , Maria-Luisa Sanchez-Millan","doi":"10.1016/j.jim.2025.113959","DOIUrl":"10.1016/j.jim.2025.113959","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113959"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Going green: boosting efficiency and sustainability with digital solutions 走向绿色：利用数字解决方案提高效率和可持续性
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113958
Jessica Murphy, Phyllis O'Connor
{"title":"Going green: boosting efficiency and sustainability with digital solutions","authors":"Jessica Murphy, Phyllis O'Connor","doi":"10.1016/j.jim.2025.113958","DOIUrl":"10.1016/j.jim.2025.113958","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113958"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Controlling background effects in anti-ganglioside antibody testing 抗神经节苷脂抗体检测中的控制背景效应
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113936
Renato Cotti , Jen Stuart , Brandon Beloin , Dominique Keller , Christian-Benedikt Gerhold , Christina Bauer
{"title":"Controlling background effects in anti-ganglioside antibody testing","authors":"Renato Cotti , Jen Stuart , Brandon Beloin , Dominique Keller , Christian-Benedikt Gerhold , Christina Bauer","doi":"10.1016/j.jim.2025.113936","DOIUrl":"10.1016/j.jim.2025.113936","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113936"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the i-Tracker infliximab and i-Tracker anti-infliximab total antibodies ECLIA on the random-access automated analyzer IDS-iSYS for the routine clinical monitoring of infliximab-treated patients 在IDS-iSYS随机存取自动分析仪上评价i-Tracker英夫利昔单抗和i-Tracker抗英夫利昔单抗总抗体ECLIA对英夫利昔单抗治疗患者的常规临床监测
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113933
Akpedje S. Dossou, Serena Kang, Tahira Kalhoro, Eduardo Castro Echeverry, Nathan C. Horton
{"title":"Evaluation of the i-Tracker infliximab and i-Tracker anti-infliximab total antibodies ECLIA on the random-access automated analyzer IDS-iSYS for the routine clinical monitoring of infliximab-treated patients","authors":"Akpedje S. Dossou, Serena Kang, Tahira Kalhoro, Eduardo Castro Echeverry, Nathan C. Horton","doi":"10.1016/j.jim.2025.113933","DOIUrl":"10.1016/j.jim.2025.113933","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113933"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis 标准抗磷脂抗体的持久性与滴度、同型和抗磷脂综合征诊断的关系
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113932
Arevik Ghazaryan , Mouhamed Yazan Abou-Ismail , Abdulrahman Saadalla
{"title":"Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis","authors":"Arevik Ghazaryan , Mouhamed Yazan Abou-Ismail , Abdulrahman Saadalla","doi":"10.1016/j.jim.2025.113932","DOIUrl":"10.1016/j.jim.2025.113932","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113932"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a sensitive sandwich ELISA for the envelope domain III protein of dengue virus type 2 登革病毒2型包膜结构域III蛋白灵敏夹心ELISA试剂盒的建立。
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.114000
Ziting Ma , Jingyi Su , Zelin Han , Ximing Ren , Jinnian Guo , Lu Jiang , Suqing Zhao
Dengue, caused by the dengue virus (DENV), is a rapidly spreading infectious disease that poses a significant global health challenge. Rapid and accurate diagnosis is critical for effective disease management and epidemic control, as dengue is a kind of vector-borne disease and lacks definitive medical treatments. Current antibodies and antigens for DENV immunoassay, including immunoglobulin G (IgG), immunoglobulin M (IgM), and non-structural protein 1 (NS1), have drawbacks like cross-reaction and false-negative results in the late stages of primary infection or secondary infection. Envelope domain III (EDIII) protein, which is immunogenic and is a recognition region and binding site for DENV receptors, can also be the potential detection target of DENV infection. However, few studies of EDIII protein as a detection target have been reported. Therefore, this study developed a sensitive and specific ELISA for DENV-2_EDIII protein detection. Specific polyclonal antibodies (pAbs) against the DENV-2_EDIII protein were generated by immunizing the New Zealand White rabbits with the recombinant DENV-2_EDIII protein. Using these pAbs, a highly sensitive and specific ELISA method was developed. The assay demonstrated a wide detection range (7.8 ng/mL to 500 ng/mL) and a low limit of detection (LOD) of 0.156 ng/mL and showed no cross-reactivity with other orthoflaviviruses. These findings may provide a new methodological framework and research direction for the diagnosis of dengue after further clinical verifications.
登革热由登革热病毒（DENV）引起，是一种迅速蔓延的传染病，对全球卫生构成重大挑战。快速和准确的诊断对于有效的疾病管理和流行病控制至关重要，因为登革热是一种媒介传播的疾病，缺乏明确的医学治疗。目前用于DENV免疫测定的抗体和抗原，包括免疫球蛋白G （IgG）、免疫球蛋白M （IgM）和非结构蛋白1 (NS1)，在原发性感染或继发性感染的晚期存在交叉反应和假阴性结果等缺点。包膜结构域III （EDIII）蛋白具有免疫原性，是DENV受体的识别区和结合位点，也可能是DENV感染的潜在检测靶点。然而，将EDIII蛋白作为检测靶点的研究报道较少。因此，本研究建立了一种灵敏、特异的DENV-2_EDIII蛋白ELISA检测方法。用重组DENV-2_EDIII蛋白免疫新西兰大白兔，获得了DENV-2_EDIII蛋白的特异性多克隆抗体（pAbs）。利用这些pAbs，建立了一种高灵敏度和特异性的ELISA方法。该方法检测范围广（7.8 ng/mL ~ 500 ng/mL），低检出限（LOD）为0.156 ng/mL，与其他正黄病毒无交叉反应性。这些发现可能为进一步临床验证登革热的诊断提供新的方法学框架和研究方向。
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引用次数: 0
Evaluation of anti-nuclear antibody (ANA) patterns and its associations with autoimmune disease biomarkers 评估抗核抗体（ANA）模式及其与自身免疫性疾病生物标志物的关联
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113924
Vincent Ricchiuti , Min Kyung Lee , David Alfego , Stanley J. Naides , Ajay Grover
{"title":"Evaluation of anti-nuclear antibody (ANA) patterns and its associations with autoimmune disease biomarkers","authors":"Vincent Ricchiuti ,&nbsp;Min Kyung Lee ,&nbsp;David Alfego ,&nbsp;Stanley J. Naides ,&nbsp;Ajay Grover","doi":"10.1016/j.jim.2025.113924","DOIUrl":"10.1016/j.jim.2025.113924","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113924"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections 同时检测马雅罗病毒和基孔肯雅病毒IgM和IgG可改善急性马雅罗病毒感染的诊断
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113939
Maite Sabalza , Julia Klemens , Marleen Janku , Erik Lattwein , Anette Schulz , Oliver Klemens
{"title":"Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections","authors":"Maite Sabalza ,&nbsp;Julia Klemens ,&nbsp;Marleen Janku ,&nbsp;Erik Lattwein ,&nbsp;Anette Schulz ,&nbsp;Oliver Klemens","doi":"10.1016/j.jim.2025.113939","DOIUrl":"10.1016/j.jim.2025.113939","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113939"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring 用于诊断和治疗监测的血管内皮生长因子- d (VEGF-D) ELISA的性能特征
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113964
Lorraine Abushanab, Maria Meriwether, Yongbao Wang, Claire Coeshott
{"title":"Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring","authors":"Lorraine Abushanab,&nbsp;Maria Meriwether,&nbsp;Yongbao Wang,&nbsp;Claire Coeshott","doi":"10.1016/j.jim.2025.113964","DOIUrl":"10.1016/j.jim.2025.113964","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113964"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity 乳糜泻风险分层：HLA基因型与乳糜泻特异性抗体阳性的相关性
IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Journal of immunological methods
Pub Date : 2025-11-01 DOI: 10.1016/j.jim.2025.113949
Tracie Profaizer , Ann Pole , Michael J. Thompson , Eszter Lazar-Molnar
{"title":"Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity","authors":"Tracie Profaizer ,&nbsp;Ann Pole ,&nbsp;Michael J. Thompson ,&nbsp;Eszter Lazar-Molnar","doi":"10.1016/j.jim.2025.113949","DOIUrl":"10.1016/j.jim.2025.113949","url":null,"abstract":"","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113949"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145416892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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