{"title":"Stereospecific Assignments of Val and Leu Methyl Groups in a Selectively13C-labeled 18 kDa Polypeptide Using 3D CT-(H)CCH–COSY and 2D1JC–CEdited Heteronuclear Correlation Experiments","authors":"Weidong Hu, Erik R.P. Zuiderweg","doi":"10.1006/jmrb.1996.0157","DOIUrl":"10.1006/jmrb.1996.0157","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 70-75"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A technique for single-shot, localized, water-suppressedin vivo1H NMR with high immunity toB1inhomogeneities is described. The single-shot localization is based on complete outer-volume suppression which is achieved with optimized, adiabatic BIR-4 pulses in combination withB0gradient modulation.B1-insensitive water suppression is achieved with BISTRO, a train of selective adiabatic full-passage pulses interleaved withB0gradient crushers. Computer simulations based on the Bloch equations, experimental images of the localized volume, andin vivo1H spectroscopy experiments demonstrate the excellent spatial localization and water suppression.
{"title":"B1-Insensitive, Single-Shot Localization and Water Suppression","authors":"R.A. de Graaf , Y. Luo , M. Garwood , K. Nicolay","doi":"10.1006/jmrb.1996.0152","DOIUrl":"10.1006/jmrb.1996.0152","url":null,"abstract":"<div><p>A technique for single-shot, localized, water-suppressed<em>in vivo</em><sup>1</sup>H NMR with high immunity to<em>B</em><sub>1</sub>inhomogeneities is described. The single-shot localization is based on complete outer-volume suppression which is achieved with optimized, adiabatic BIR-4 pulses in combination with<em>B</em><sub>0</sub>gradient modulation.<em>B</em><sub>1</sub>-insensitive water suppression is achieved with BISTRO, a train of selective adiabatic full-passage pulses interleaved with<em>B</em><sub>0</sub>gradient crushers. Computer simulations based on the Bloch equations, experimental images of the localized volume, and<em>in vivo</em><sup>1</sup>H spectroscopy experiments demonstrate the excellent spatial localization and water suppression.</p></div>","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 35-45"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Signal-to-Noise and Magnetic Susceptibility Trade-offs in Solenoidal Microcoils for NMR","authors":"A.G. Webb, S.C. Grant","doi":"10.1006/jmrb.1996.0159","DOIUrl":"10.1006/jmrb.1996.0159","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 83-87"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of a Proton-Detected–Carbon-Displayed HSQC-INADEQUATE with Carbon-Detected INADEQUATE Data on a Natural Product","authors":"T.K. Pratum","doi":"10.1006/jmrb.1996.0158","DOIUrl":"10.1006/jmrb.1996.0158","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 76-82"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76240203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Potter , R.L. Kleinberg , F.J. Brockman , E.W. Mcfarland
In this work, an NMR technique capable of detecting bacterial cells and measuring the cell density in suspension and in porous media has been developed. It is based on the pulsed-field-gradient technique and relies on the fact that extracellular water diffuses freely while intracellular water is completely restricted by the relatively impermeable cell wall of the bacterium. At high wave vectors, the signal from extracellular water is completely suppressed while the signal from intracellular water is comparatively unaffected. This technique has been applied to the mapping of bacterial distributions in porous media. This method is presented as a non-destructive, real-time technique for biomass characterization within laboratory column and flow cell experiments, and possibly for monitoring in situ bioremediation.
{"title":"Assay for Bacteria in Porous Media by Diffusion-Weighted NMR","authors":"K. Potter , R.L. Kleinberg , F.J. Brockman , E.W. Mcfarland","doi":"10.1006/jmrb.1996.0149","DOIUrl":"10.1006/jmrb.1996.0149","url":null,"abstract":"In this work, an NMR technique capable of detecting bacterial cells and measuring the cell density in suspension and in porous media has been developed. It is based on the pulsed-field-gradient technique and relies on the fact that extracellular water diffuses freely while intracellular water is completely restricted by the relatively impermeable cell wall of the bacterium. At high wave vectors, the signal from extracellular water is completely suppressed while the signal from intracellular water is comparatively unaffected. This technique has been applied to the mapping of bacterial distributions in porous media. This method is presented as a non-destructive, real-time technique for biomass characterization within laboratory column and flow cell experiments, and possibly for monitoring in situ bioremediation.","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 9-15"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroshi Matsuo , Ēriks Kupce , Hanjun Li , Gerhard Wagner
{"title":"Increased Sensitivity in HNCA and HN(CO)CA Experiments by Selective CβDecoupling","authors":"Hiroshi Matsuo , Ēriks Kupce , Hanjun Li , Gerhard Wagner","doi":"10.1006/jmrb.1996.0161","DOIUrl":"10.1006/jmrb.1996.0161","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 91-96"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of Backbone Torsion Angle ϵ of DNA Duplexes in Solution from3JC4′Pand3JH3′P, Using Heteronuclear Single-Quantum-Coherence Spectroscopy","authors":"Sik Lok Lam, Steve C.F. Au-Yeung","doi":"10.1006/jmrb.1996.0155","DOIUrl":"10.1006/jmrb.1996.0155","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 59-64"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stefan Widmaier , Thomas Hoess , Johannes Breuer , Wulf-Ingo Jung , Günther J. Dietze , Otto Lutz
Proton-decoupled31P NMR spectroscopy was performedin vitroandin vivoin humans to investigate the temperature dependence of the chemical-shift separation δαβbetween the α- and β-phosphorus resonances and the31P–31P coupling constants of ATP using a 1.5 T whole-body imager.In vitroin the presence of magnesium, a linear temperature dependence of δαβwas found that varied systematically with Mg-to-ATP ratio (R). This needs to be considered in studies where δαβis used to determine temperature. A second important point is the temperature dependence of −0.0128 ± 0.0002 ppm/°C (mean ± SD,n= 6) of the chemical-shift limit δMgATPαβ(T) of mostly mono-magnesium-complexed ATP found atR= 2.2 ± 0.2. This parameter is used in combination with the temperature dependences of the uncomplexed limit δATPαβ(T) and the dissociation constantKMgATPD(T) for the determination of [Mg2+free] at different temperatures. Preliminary studiesin vivoshow that δαβalso decreases in human skeletal muscle with increasing temperature, similarly to thein vitroresults. Therefore, interpretations of differences in δαβfoundin vivomust consider even small temperature differences to avoid significant errors in the determination of [Mg2+free].
{"title":"Magnesium-Based Temperature Dependence of the ATP Chemical-Shift Separation δαβand Its Relation to Intracellular Free Magnesium","authors":"Stefan Widmaier , Thomas Hoess , Johannes Breuer , Wulf-Ingo Jung , Günther J. Dietze , Otto Lutz","doi":"10.1006/jmrb.1996.0150","DOIUrl":"10.1006/jmrb.1996.0150","url":null,"abstract":"<div><p>Proton-decoupled<sup>31</sup>P NMR spectroscopy was performed<em>in vitro</em>and<em>in vivo</em>in humans to investigate the temperature dependence of the chemical-shift separation δ<sub>αβ</sub>between the α- and β-phosphorus resonances and the<sup>31</sup>P–<sup>31</sup>P coupling constants of ATP using a 1.5 T whole-body imager.<em>In vitro</em>in the presence of magnesium, a linear temperature dependence of δ<sub>αβ</sub>was found that varied systematically with Mg-to-ATP ratio (<em>R</em>). This needs to be considered in studies where δ<sub>αβ</sub>is used to determine temperature. A second important point is the temperature dependence of −0.0128 ± 0.0002 ppm/°C (mean ± SD,<em>n</em>= 6) of the chemical-shift limit δ<sup>MgATP</sup><sub>αβ</sub>(<em>T</em>) of mostly mono-magnesium-complexed ATP found at<em>R</em>= 2.2 ± 0.2. This parameter is used in combination with the temperature dependences of the uncomplexed limit δ<sup>ATP</sup><sub>αβ</sub>(<em>T</em>) and the dissociation constant<em>K</em><sup>MgATP</sup><sub>D</sub>(<em>T</em>) for the determination of [Mg<sup>2+</sup><sub>free</sub>] at different temperatures. Preliminary studies<em>in vivo</em>show that δ<sub>αβ</sub>also decreases in human skeletal muscle with increasing temperature, similarly to the<em>in vitro</em>results. Therefore, interpretations of differences in δ<sub>αβ</sub>found<em>in vivo</em>must consider even small temperature differences to avoid significant errors in the determination of [Mg<sup>2+</sup><sub>free</sub>].</p></div>","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 16-24"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76613525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multislice Imaging with Adiabatic Pulses Using Transverse Hadamard Encoding","authors":"Robin A. de Graaf, Klaas Nicolay","doi":"10.1006/jmrb.1996.0162","DOIUrl":"10.1006/jmrb.1996.0162","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 97-101"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85478421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimal Field Strength and Pulse-Sequence Parameters forin VivoDynamic-Polarization Imaging","authors":"H. Konijnenburg, A.F. Mehlkopf","doi":"10.1006/jmrb.1996.0154","DOIUrl":"10.1006/jmrb.1996.0154","url":null,"abstract":"","PeriodicalId":16130,"journal":{"name":"Journal of Magnetic Resonance, Series B","volume":"113 1","pages":"Pages 53-58"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/jmrb.1996.0154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80537764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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