{"title":"Developing Stakeholder Dynamic Assessment for developing wild animals conservation: an evidence from West New Guinea","authors":"","doi":"10.7176/jnsr/15-1-06","DOIUrl":"https://doi.org/10.7176/jnsr/15-1-06","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hepatoprotective Effect of Methanol Stem Bark Extract of Parkia biglobosa in Wister albino Rats","authors":"","doi":"10.7176/jnsr/15-1-05","DOIUrl":"https://doi.org/10.7176/jnsr/15-1-05","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140520902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CLIMATE CHANGE IMPACT, ITS ADAPTATION MEASURES ON FISHERMEN LIVELIHOOD STATUS IN COASTAL AREA OF SOUTHWEST NIGERIA","authors":"","doi":"10.7176/jnsr/14-12-07","DOIUrl":"https://doi.org/10.7176/jnsr/14-12-07","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139292678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weissella species are a group of lactic acid bacteria gaining rapid popularity as a result of discoveries centered on their biotechnological properties. In this study, an in silico approach was imbibed to investigate the antimicrobial potentials of metabolites of Weissella ciberia . Soluble compounds of W. ciberia were subjected to High Performance Liquid Chromatographic (HPLC) analysis and the inherent metabolites were identified. In order to evaluate their antimicrobial potentials against Escherichia coli and Shigella flexneri , the identified metabolites of W. ciberia were further subjected to geometry optimization of compound structures, ligand/receptor preparation, docking calculations and docking simulations. The HPLC identified metabolites from W. ciberia were atropoine, gallic acid, naringinin, caffeine, maleic acid, saponin and glutathione. The results of the in silico analysis showed binding affinities of the metabolites against the target microorganisms at a range of ˗4.6 to 10.7 Kcal/mol. Among metabolites, the highest binding affinity was observed in saponin against E. coli and S. flexneri at scores of ˗9.7 Kcal/mol and ˗10 Kcal/mol respectively. Binding affinities against E. coli and S. flexneri were also observed in naringinin at binding scores of ˗7.8 Kcal/mol and ˗8.5 Kcal/mol respectively. The scores obtained in this study predicts strong antimicrobial potentials that were comparable to those of conventional antibiotics such as ciprofloxacin and meropenem. Hence, the antimicrobial activities of metabolites of W. ciberia could be harnessed further for their potential in drug sensitivity against multiple-drug resistant pathogenic microbes. KEYWORDS : Bioactive, metabolites, fermentate, in silico and binding affinity. DOI: 10.7176/JNSR/14-12-06 Publication date: October 30 th 2023
{"title":"In silico evaluation of the antimicrobial potentials of soluble bioactive compounds derived from Weissella ciberia metabolites.","authors":"","doi":"10.7176/jnsr/14-12-06","DOIUrl":"https://doi.org/10.7176/jnsr/14-12-06","url":null,"abstract":"Weissella species are a group of lactic acid bacteria gaining rapid popularity as a result of discoveries centered on their biotechnological properties. In this study, an in silico approach was imbibed to investigate the antimicrobial potentials of metabolites of Weissella ciberia . Soluble compounds of W. ciberia were subjected to High Performance Liquid Chromatographic (HPLC) analysis and the inherent metabolites were identified. In order to evaluate their antimicrobial potentials against Escherichia coli and Shigella flexneri , the identified metabolites of W. ciberia were further subjected to geometry optimization of compound structures, ligand/receptor preparation, docking calculations and docking simulations. The HPLC identified metabolites from W. ciberia were atropoine, gallic acid, naringinin, caffeine, maleic acid, saponin and glutathione. The results of the in silico analysis showed binding affinities of the metabolites against the target microorganisms at a range of ˗4.6 to 10.7 Kcal/mol. Among metabolites, the highest binding affinity was observed in saponin against E. coli and S. flexneri at scores of ˗9.7 Kcal/mol and ˗10 Kcal/mol respectively. Binding affinities against E. coli and S. flexneri were also observed in naringinin at binding scores of ˗7.8 Kcal/mol and ˗8.5 Kcal/mol respectively. The scores obtained in this study predicts strong antimicrobial potentials that were comparable to those of conventional antibiotics such as ciprofloxacin and meropenem. Hence, the antimicrobial activities of metabolites of W. ciberia could be harnessed further for their potential in drug sensitivity against multiple-drug resistant pathogenic microbes. KEYWORDS : Bioactive, metabolites, fermentate, in silico and binding affinity. DOI: 10.7176/JNSR/14-12-06 Publication date: October 30 th 2023","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136198906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dialium guineense or African black velvet tamarind, is a common tree in West Africa whose parts have been established for its antimicrobial and therapeutic properties. In this study, the stem bark of the plant was accessed for endophytic bacteria and their activity against common clinical isolates were evaluated. Isolation of bacteria endophytes from the stem bark of the plant was achieved by surface sterilization using 70% ethanol and 2% sodium hypochlorite before aseptically cutting into small sizes of about 3.5 - 4.0 mm, plated on nutrient agar and then incubated for 24 hours. Pure isolates of the endophytes were obtained and identified macroscopically and molecularly by depositing 16SrRNA sequences of all the isolates on NCBI website. The endophytic bacteria isolates belong to the genera Pseudomonas, Halopseudomonas, Burkholderia, Streptococcus and Bacillus. Antibacterial activity carried out with the crude extracts of all endophytic bacteria isolates revealed that the bacteria endophyte mSB2 of the genera Halospeudomonas, had the most clearer zones and diameter of inhibition against all test isolates with zones ranging from 9.0 ± 0.35 mm to 35 ± 0.5 mm; hence, the extract from mSB2 was most active and posed effective antibacterial activities. This study established a fact that Dialium guineesnse harbors bacteria endophytes with active metabolites against common disease-causing organisms. Keywords: Dialium guineense, Endophytic bacteria, Stem bark, Antibacterial activities, Metabolites. DOI: 10.7176/JNSR/14-12-04 Publication date: September 30 th 2023
几内亚黑绒罗望子是西非的一种常见树木，其部分因其抗菌和治疗特性而被确定。在本研究中，对该植物的茎皮进行了内生细菌的提取，并对其对常见临床分离株的活性进行了评价。用70%乙醇和2%次氯酸钠表面灭菌，分离出植物茎皮内生菌，然后无菌切成3.5 - 4.0 mm的小块，镀在营养琼脂上孵育24小时。将所有内生菌的16SrRNA序列保存在NCBI网站上，获得了内生菌的纯分离株，并进行了宏观和分子鉴定。分离的内生细菌属假单胞菌属、假假单胞菌属、伯克氏菌属、链球菌属和芽孢杆菌属。对各内生菌粗提物进行抑菌活性研究表明，盐藻单胞菌属内生菌mSB2对各菌株的抑菌带和抑菌直径最清晰，抑菌带范围为9.0±0.35 mm ~ 35±0.5 mm;因此，mSB2的提取物活性最高，具有有效的抗菌活性。本研究证实了豚鼠Dialium含有细菌内生菌，其代谢产物对常见的致病生物具有活性。关键词:豚草，内生细菌，茎皮，抗菌活性，代谢物出版日期:2023年9月30日
{"title":"Antibacterial Activity of Endophytic Bacteria from Stem Bark of Dialium guineense (Wild).","authors":"","doi":"10.7176/jnsr/14-12-04","DOIUrl":"https://doi.org/10.7176/jnsr/14-12-04","url":null,"abstract":"Dialium guineense or African black velvet tamarind, is a common tree in West Africa whose parts have been established for its antimicrobial and therapeutic properties. In this study, the stem bark of the plant was accessed for endophytic bacteria and their activity against common clinical isolates were evaluated. Isolation of bacteria endophytes from the stem bark of the plant was achieved by surface sterilization using 70% ethanol and 2% sodium hypochlorite before aseptically cutting into small sizes of about 3.5 - 4.0 mm, plated on nutrient agar and then incubated for 24 hours. Pure isolates of the endophytes were obtained and identified macroscopically and molecularly by depositing 16SrRNA sequences of all the isolates on NCBI website. The endophytic bacteria isolates belong to the genera Pseudomonas, Halopseudomonas, Burkholderia, Streptococcus and Bacillus. Antibacterial activity carried out with the crude extracts of all endophytic bacteria isolates revealed that the bacteria endophyte mSB2 of the genera Halospeudomonas, had the most clearer zones and diameter of inhibition against all test isolates with zones ranging from 9.0 ± 0.35 mm to 35 ± 0.5 mm; hence, the extract from mSB2 was most active and posed effective antibacterial activities. This study established a fact that Dialium guineesnse harbors bacteria endophytes with active metabolites against common disease-causing organisms. Keywords: Dialium guineense, Endophytic bacteria, Stem bark, Antibacterial activities, Metabolites. DOI: 10.7176/JNSR/14-12-04 Publication date: September 30 th 2023","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135641264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: As the main arterial supply of the upper limb, brachial artery (BA) terminates by dividing into ulnar and radial artery about 1cm at the neck of radius as documented in standard anatomy text books. However, due to variations in results from different studies, BA may terminate at the level of neck of radius, radial tuberosity, mid arm and proximal arm. Based on its clinical utility such as blood pressure monitoring and surgical procedures, few reported disparities in certain populations and paucity data especially in black African population, exploration of variations in termination of BA is warranted. Objective :The purpose of this study was to evaluate variation in termination of brachial artery among black African population. Methodology: This was a cross sectional descriptive study carried out in human anatomy laboratories in Maseno, Uzima and Masinde muliro universities in Western Kenya. In this study, 77 cadavers constituting (n=154) upper limb specimens of back African population were sampled using stratified sampling technique. Data on termination of BA, laterality of the upper limb and sex of the cadaver were recorded in data entry form. Brachium region was exposed to access the brachial artery where its course to termination was assessed. Descriptive statistics was used to assess frequency distribution of variant termination while Chi-square was used to determine difference in proportion of normal termination and cumulative variation of termination of BA with regards to laterality of the upper limb. Results: Out of 154 upper limbs studied, the majority (89.0%) had a normal termination at the radial neck, while 7.8% terminated at the radial tuberosity. A small percentage of the upper limbs (1.3% and 1.9%) had termination at midarm and proximal arm, respectively. There was no statistically significant difference in variation in the left and right limbs (p=0.333 and p= 0.564) respectively relative to the normal termination. Conclusion and recommendations: There are variations in termination of brachial artery among the black African population, however, the variation from the normal morphology is not statistically significant, though clinically significant. Termination at radial tuberosity is the most common variant and more common in men than women. Understanding variant termination of BA among black African population is key to all health care professionals especially surgeons, radiologists, anatomists and medical students as such variants may lead to misdiagnosis and post operative related complications. Thus, further population and race specific studies need to be undertaken on such variants. Keywords: Brachial artey, Ulnar artery, Radial artery, Radial tuberosity, Radial neck, Midarm. DOI: 10.7176/JNSR/14-12-03 Publication date: September 30 th 2023
{"title":"Variation in Termination of Brachial Artery Among Black African Population","authors":"","doi":"10.7176/jnsr/14-12-03","DOIUrl":"https://doi.org/10.7176/jnsr/14-12-03","url":null,"abstract":"Background: As the main arterial supply of the upper limb, brachial artery (BA) terminates by dividing into ulnar and radial artery about 1cm at the neck of radius as documented in standard anatomy text books. However, due to variations in results from different studies, BA may terminate at the level of neck of radius, radial tuberosity, mid arm and proximal arm. Based on its clinical utility such as blood pressure monitoring and surgical procedures, few reported disparities in certain populations and paucity data especially in black African population, exploration of variations in termination of BA is warranted. Objective :The purpose of this study was to evaluate variation in termination of brachial artery among black African population. Methodology: This was a cross sectional descriptive study carried out in human anatomy laboratories in Maseno, Uzima and Masinde muliro universities in Western Kenya. In this study, 77 cadavers constituting (n=154) upper limb specimens of back African population were sampled using stratified sampling technique. Data on termination of BA, laterality of the upper limb and sex of the cadaver were recorded in data entry form. Brachium region was exposed to access the brachial artery where its course to termination was assessed. Descriptive statistics was used to assess frequency distribution of variant termination while Chi-square was used to determine difference in proportion of normal termination and cumulative variation of termination of BA with regards to laterality of the upper limb. Results: Out of 154 upper limbs studied, the majority (89.0%) had a normal termination at the radial neck, while 7.8% terminated at the radial tuberosity. A small percentage of the upper limbs (1.3% and 1.9%) had termination at midarm and proximal arm, respectively. There was no statistically significant difference in variation in the left and right limbs (p=0.333 and p= 0.564) respectively relative to the normal termination. Conclusion and recommendations: There are variations in termination of brachial artery among the black African population, however, the variation from the normal morphology is not statistically significant, though clinically significant. Termination at radial tuberosity is the most common variant and more common in men than women. Understanding variant termination of BA among black African population is key to all health care professionals especially surgeons, radiologists, anatomists and medical students as such variants may lead to misdiagnosis and post operative related complications. Thus, further population and race specific studies need to be undertaken on such variants. Keywords: Brachial artey, Ulnar artery, Radial artery, Radial tuberosity, Radial neck, Midarm. DOI: 10.7176/JNSR/14-12-03 Publication date: September 30 th 2023","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135641265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An integrated reflection seismic and well log data interpretations of reservoirs in the ‘M’ Field located in onshore Niger Delta were carried out to map the complex subsurface structures and characterize the reservoirs in order to predict the hydrocarbon potential of the field. The methodology includes delineation and assessment of rock properties of reservoirs from logs of five wells, faults and horizons mapping from 3D seismic data, time to depth conversion, generation of time and depth structural maps of identified reservoirs. Structural interpretation shows presence of five main faults (F1-F5) while petrophysical evaluation delineated two hydrocarbon bearing zones (reservoirs 1 and 2). The quality of the delineated reservoirs in the ''M-field'' Niger Delta is good as revealed by the various petrophysical parameters estimated. The reservoirs are also considerably thick to host hydrocarbon in commercial quantities. The exploratory wells are producible as shown by the values estimated for water saturation and hydrocarbon saturation. The time and depth structure maps shows that the identified faults are fault dependent and fault-assisted closures and are thus regarded as good hydrocarbon traps Keywords: Niger Delta, reservoir, hydrocarbon trap, structural map DOI: 10.7176/JNSR/14-12-05 Publication date: September 30 th 2023
{"title":"3D Structural Interpretation and Petrophysical Evaluation of Reservoirs in the ‘M’ Field, Onshore Niger Delta, Nigeria.","authors":"","doi":"10.7176/jnsr/14-12-05","DOIUrl":"https://doi.org/10.7176/jnsr/14-12-05","url":null,"abstract":"An integrated reflection seismic and well log data interpretations of reservoirs in the ‘M’ Field located in onshore Niger Delta were carried out to map the complex subsurface structures and characterize the reservoirs in order to predict the hydrocarbon potential of the field. The methodology includes delineation and assessment of rock properties of reservoirs from logs of five wells, faults and horizons mapping from 3D seismic data, time to depth conversion, generation of time and depth structural maps of identified reservoirs. Structural interpretation shows presence of five main faults (F1-F5) while petrophysical evaluation delineated two hydrocarbon bearing zones (reservoirs 1 and 2). The quality of the delineated reservoirs in the ''M-field'' Niger Delta is good as revealed by the various petrophysical parameters estimated. The reservoirs are also considerably thick to host hydrocarbon in commercial quantities. The exploratory wells are producible as shown by the values estimated for water saturation and hydrocarbon saturation. The time and depth structure maps shows that the identified faults are fault dependent and fault-assisted closures and are thus regarded as good hydrocarbon traps Keywords: Niger Delta, reservoir, hydrocarbon trap, structural map DOI: 10.7176/JNSR/14-12-05 Publication date: September 30 th 2023","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135735320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plasmodium falciparum Infection Modulates Platelet Count than Leucocyte Parameters in Carriage of Different Haemoglobin Beta Subunit (HBB) Genotypes","authors":"","doi":"10.7176/jnsr/14-10-05","DOIUrl":"https://doi.org/10.7176/jnsr/14-10-05","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90438339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Cannabidiol Oil and Prednisolone on Sex Hormones, Oxidative Stress Markers, Lipid Profile, and Testes Histology on Cadmium Induced Toxicity in Male Wistar Rats","authors":"","doi":"10.7176/jnsr/14-10-03","DOIUrl":"https://doi.org/10.7176/jnsr/14-10-03","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77180856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Impacts of Salt Affected Soil on Soil and Plant Growth and Management Options with Organic and Inorganic Amendments A: Reviewv","authors":"","doi":"10.7176/jnsr/14-10-02","DOIUrl":"https://doi.org/10.7176/jnsr/14-10-02","url":null,"abstract":"","PeriodicalId":16368,"journal":{"name":"Journal of Natural Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90716033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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