Mustard Is an Important Oilseed Which Is Used For many Purposes Like Culinary, Religious And Cultural Purposes By Humanity Since Time Immemorial. To Increase The Production of Mustard, Phytohormones Can Be Used Which Are Pro1duced With The Help Of Microorganisms. Phytohormones are the signaling or organic compound which helps in the development and growth of plant. Rhizobium and Bacillus sp were used to produce phytohormones which are isolated from different sources and investigated for their ability to produced auxin and cytokinin in PM2 and Czapek medium respectively. Thin layer chromatography and High performance liquid chromatography were performed to detect and quantify the amount of hormones in the production medium. Rhizobium produced the highest auxin (0.087mg/l) and cytokinin (0.047mg/ml) in the production medium. Then these microbiologically synthesized auxin and cytokinin were treated with mustard seeds to check their ability in promoting growth development. Auxin produced from microorganisms comparatively showed the fastest shoot development (7.9cm length) and leaf (length-1.8cm and width-1.9cm) than cytokinin and control.
{"title":"Testing the efficacy of phytohormones of microbial origin in promoting plant growth","authors":"V. Mahalakshmi, Ditisha Bhagawati","doi":"10.24896/JMBR.2017711","DOIUrl":"https://doi.org/10.24896/JMBR.2017711","url":null,"abstract":"Mustard Is an Important Oilseed Which Is Used For many Purposes Like Culinary, Religious And Cultural Purposes By Humanity Since Time Immemorial. To Increase The Production of Mustard, Phytohormones Can Be Used Which Are Pro1duced With The Help Of Microorganisms. Phytohormones are the signaling or organic compound which helps in the development and growth of plant. Rhizobium and Bacillus sp were used to produce phytohormones which are isolated from different sources and investigated for their ability to produced auxin and cytokinin in PM2 and Czapek medium respectively. Thin layer chromatography and High performance liquid chromatography were performed to detect and quantify the amount of hormones in the production medium. Rhizobium produced the highest auxin (0.087mg/l) and cytokinin (0.047mg/ml) in the production medium. Then these microbiologically synthesized auxin and cytokinin were treated with mustard seeds to check their ability in promoting growth development. Auxin produced from microorganisms comparatively showed the fastest shoot development (7.9cm length) and leaf (length-1.8cm and width-1.9cm) than cytokinin and control.","PeriodicalId":16482,"journal":{"name":"Journal of Microbiology and Biotechnology Research","volume":"5 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87848456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Singh, R. Gaur, S. Bansal, Farrukh Jamal, P. Pandey, S. Tiwari, S. Sarsaiya, Saket Mishra, N. Chaturvedi, D. P. Singh, M. K. Gaur, Father G. Vazhan Arasu
Aureobasidium pullulans, popularly known as black yeast, is one of the most widespread saprophytic fungus associated with wide range of terrestrial and aquatic habitats. The fungus has widely been employed in production of an economically important polysaccharide pullulan. Total 50 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 10 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RS-4, produced highest pullulan (6.0±0.003 g/100ml) at 43oC, pH 5.0 in 72h of incubation with 5% sucrose and 0.5% yeast extract in a non-stirred flask fermentation system with working volume 100ml. Key Words: Aureobasidium pullulans, Pullulan, Production, Flask Fermentation System
{"title":"Production of Pullulan from a high yielding strain of Aureobasidium pullulans in non-stirred flask-type fermentation system","authors":"R. Singh, R. Gaur, S. Bansal, Farrukh Jamal, P. Pandey, S. Tiwari, S. Sarsaiya, Saket Mishra, N. Chaturvedi, D. P. Singh, M. K. Gaur, Father G. Vazhan Arasu","doi":"10.24896/JMBR.2017715","DOIUrl":"https://doi.org/10.24896/JMBR.2017715","url":null,"abstract":"Aureobasidium pullulans, popularly known as black yeast, is one of the most widespread saprophytic fungus associated with wide range of terrestrial and aquatic habitats. The fungus has widely been employed in production of an economically important polysaccharide pullulan. Total 50 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 10 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RS-4, produced highest pullulan (6.0±0.003 g/100ml) at 43oC, pH 5.0 in 72h of incubation with 5% sucrose and 0.5% yeast extract in a non-stirred flask fermentation system with working volume 100ml. Key Words: Aureobasidium pullulans, Pullulan, Production, Flask Fermentation System","PeriodicalId":16482,"journal":{"name":"Journal of Microbiology and Biotechnology Research","volume":"25 1","pages":"26-32"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74393651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cocoa (Theobroma cacao L.) is a cash crop of huge economic significance in the world and the key raw material for chocolate manufacturing. Polyphenol oxidases (PPOs) are copper containing oxidoreductases that catalyze the hydroxylation and oxidation of phenolic compounds in the presence of molecular oxygen. The PPO presence and activity during fermentation and drying of cocoa beans is responsible for the development of flavor precursors. In this study, bioinformatics analysisof PPO from cocoa was done using standard bioinformatics tools such as Blastp, Hmmer, ClustalO, OMABrowser, EMBOSS and Swissmodel. The result showed that cocoa PPO homologs include Citrus sinensis, Populus euphratica, Gossypium raimondii, Litchi chinensis, Dimocarpuslongan, Canarium album, while the orthologsobtainedinclude Sorghum bicolor, Zea may, Manihot esculenta and Vitis vinifera among others.PPO of Solanum lycopersicum (tomato) was found to be a distant homolog of cocoa PPO, and that this evolutionary relationship implicated Witches’ Broom Disease (WBD). The computed physicochemical properties were in alignment with the experimental results. Keywords: Polyphenol Oxidase, Cocoa, Theobroma cacao L., Computational Analysis, Experimental Analysis.
{"title":"Comparative Evaluation of Computational and Experimental Analysis of Polyphenol Oxidase from Cocoa (Theobroma cacao L.)","authors":"D. M. Sanni, T. Fatoki, O. Omotoyinbo","doi":"10.24896/JMBR.2017714","DOIUrl":"https://doi.org/10.24896/JMBR.2017714","url":null,"abstract":"Cocoa (Theobroma cacao L.) is a cash crop of huge economic significance in the world and the key raw material for chocolate manufacturing. Polyphenol oxidases (PPOs) are copper containing oxidoreductases that catalyze the hydroxylation and oxidation of phenolic compounds in the presence of molecular oxygen. The PPO presence and activity during fermentation and drying of cocoa beans is responsible for the development of flavor precursors. In this study, bioinformatics analysisof PPO from cocoa was done using standard bioinformatics tools such as Blastp, Hmmer, ClustalO, OMABrowser, EMBOSS and Swissmodel. The result showed that cocoa PPO homologs include Citrus sinensis, Populus euphratica, Gossypium raimondii, Litchi chinensis, Dimocarpuslongan, Canarium album, while the orthologsobtainedinclude Sorghum bicolor, Zea may, Manihot esculenta and Vitis vinifera among others.PPO of Solanum lycopersicum (tomato) was found to be a distant homolog of cocoa PPO, and that this evolutionary relationship implicated Witches’ Broom Disease (WBD). The computed physicochemical properties were in alignment with the experimental results. Keywords: Polyphenol Oxidase, Cocoa, Theobroma cacao L., Computational Analysis, Experimental Analysis.","PeriodicalId":16482,"journal":{"name":"Journal of Microbiology and Biotechnology Research","volume":"126 1","pages":"18-25"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88137975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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