Decidual transformation of the endometrium is the final stage of the cyclic endometrial transformation during pregnancy. The morphogenesis of endometrial transformation is represented by clearly coordinated hormone-receptor interactions via immunological, angiogenic, and apoptotic factors that are necessary for pregnancy development. The pathology of morphogenesis can cause implantation disorders and early reproductive losses, highlighting the medical and social relevance of this issue. The aim of this review was to analyze the literature data on the morphogenesis of endometrial transformation in the first trimester of pregnancy. Open access full-text publications from the PubMed and eLibrary databases, as well as Russian relevant journals from 1999 to 2021 were used for the analysis. The morphogenesis of endometrial transformation in the first trimester of pregnancy is determined by cyclic endometrial transformation adequacy and the optimal endometrium-embryo interaction under the influence of many factors. The complex approach in evaluation of multiple links in the morphogenesis of decidual transformation of the endometrium (steroidogenesis, immunogenesis, angiogenesis, and apoptosis) will reveal molecular mechanisms of pregnancy termination due to altered decidual membrane formation. The fundamental value of molecular mechanisms underlying endometrial transformation in understanding the pathogenesis of reproductive failures dictates the necessity of endometrial transformation investigation under conditions of reprogramming and remodeling during pregnancy.
{"title":"Morphogenesis of decidual transformation of the endometrium. A literature review","authors":"Tat'yna G. Tral', Darya D. Kruglova, G. Tolibova","doi":"10.17816/jowd531483","DOIUrl":"https://doi.org/10.17816/jowd531483","url":null,"abstract":"Decidual transformation of the endometrium is the final stage of the cyclic endometrial transformation during pregnancy. The morphogenesis of endometrial transformation is represented by clearly coordinated hormone-receptor interactions via immunological, angiogenic, and apoptotic factors that are necessary for pregnancy development. The pathology of morphogenesis can cause implantation disorders and early reproductive losses, highlighting the medical and social relevance of this issue. The aim of this review was to analyze the literature data on the morphogenesis of endometrial transformation in the first trimester of pregnancy. Open access full-text publications from the PubMed and eLibrary databases, as well as Russian relevant journals from 1999 to 2021 were used for the analysis. The morphogenesis of endometrial transformation in the first trimester of pregnancy is determined by cyclic endometrial transformation adequacy and the optimal endometrium-embryo interaction under the influence of many factors. The complex approach in evaluation of multiple links in the morphogenesis of decidual transformation of the endometrium (steroidogenesis, immunogenesis, angiogenesis, and apoptosis) will reveal molecular mechanisms of pregnancy termination due to altered decidual membrane formation. The fundamental value of molecular mechanisms underlying endometrial transformation in understanding the pathogenesis of reproductive failures dictates the necessity of endometrial transformation investigation under conditions of reprogramming and remodeling during pregnancy.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139244063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Molotkov, M. Yarmolinskaya, A. A. Tsypurdeeva, M. Shalina
The incidence of adenomyosis in young patients who have not realized their reproductive function is steadily increasing, with the tactics of surgical and drug treatment being an unresolved problem. In clinical practice, special attention is focused on the possibility of surgical treatment of adenomyosis with preservation of the uterus; and a variety of new options for such operations are proposed, as well as ways to optimize the treatment of such patients. This article summarizes our own and world experience in managing such patients. We present current views regarding the selection of patients for such interventions and determining the indications for surgery, and we herein highlight the technical features of such interventions. A particular attention is paid to management tactics being aimed at ensuring reproductive function implementation.
{"title":"Modern approaches to performing organ-preserving operations in adenomyosis","authors":"A. Molotkov, M. Yarmolinskaya, A. A. Tsypurdeeva, M. Shalina","doi":"10.17816/jowd339433","DOIUrl":"https://doi.org/10.17816/jowd339433","url":null,"abstract":"The incidence of adenomyosis in young patients who have not realized their reproductive function is steadily increasing, with the tactics of surgical and drug treatment being an unresolved problem. In clinical practice, special attention is focused on the possibility of surgical treatment of adenomyosis with preservation of the uterus; and a variety of new options for such operations are proposed, as well as ways to optimize the treatment of such patients. This article summarizes our own and world experience in managing such patients. We present current views regarding the selection of patients for such interventions and determining the indications for surgery, and we herein highlight the technical features of such interventions. A particular attention is paid to management tactics being aimed at ensuring reproductive function implementation.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84063573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Chronic disorders of consciousness are rare clinical conditions that develop after coma and are accompanied by the restoration of wakefulness without the full restoration of consciousness 28 days or longer after brain damage. All women with chronic disorders of consciousness have menstrual irregularities such as oligomenorrhea or secondary amenorrhea. Clinical experience shows that recovery of the menstrual response in a number of patients with chronic disorders of consciousness precedes or is combined with recovery of clear consciousness. The results of a comprehensive examination of patients with different types of ovarian failure, depending on the type of chronic disorders of consciousness, have not been presented in the literature. Therefore, an analysis of the results of such an examination can become the basis for the selection of pathogenetically substantiated hormone-modulating therapy and the development of predictive models for assessing recovery of consciousness from chronic disorders of consciousness. AIM: The aim of this study was to assess the variants of ovarian failure in patients with different types of chronic disorders of consciousness and to develop prognostic models that evaluate the possibility of restoring consciousness. MATERIALS AND METHODS: This study enrolled 30 patients aged 18 to 44 years divided into three groups depending on the level of consciousness: vegetative state / unresponsive wakefulness syndrome (n = 12), minimally conscious state minus (n = 6), and minimally conscious state plus (n = 12). Here we examined lipid profile changes (total cholesterol, low-density lipoprotein, high-density lipoprotein) and levels of follicle-stimulating hormone, luteinizing hormone, prolactin, total testosterone, estradiol, free triiodothyronine, thyroxine, thyroid-stimulating hormone, adrenocorticotropic hormone, cortisol, and vitamin 25(OH)D in the blood, as well as levels of melatonin in the blood serum and 6-sulfatoxymelatonin in the urine. In addition, in the blood serum and cerebrospinal fluid, we evaluated levels of brain-derived neurotrophic factor, apoptosis antigen 1, Fas-ligand, glutamate, and S100 protein. We also performed ultrasound of the pelvic organs and mammary glands, magnetic resonance imaging of the chiasmal-sellar region, cervical screening, and genetic study (the prothrombin gene mutation and the Leiden factor). RESULTS: Hypogonadotropic and normogonadotropic ovarian failures were detected in 36.7% and 63.3% of patients, respectively. No differences in neurotrophin levels between the study groups were found. Three mathematical models were formulated for predicting the recovery of patients into a clear consciousness using the levels of thyroid-stimulating hormone, prolactin, follicle-stimulating hormone, anti-Mllerian hormone, total testosterone, and the age of patients. CONCLUSIONS: The presented prognostic models by determining hormone levels in the blood plasma allow for calculating the o
{"title":"The hormonal status and the possibility of predicting outcomes in patients with different types of chronic disorders of consciousness","authors":"A. O. Ivanova, M. Yarmolinskaya, E. Kondratyeva","doi":"10.17816/jowd370684","DOIUrl":"https://doi.org/10.17816/jowd370684","url":null,"abstract":"BACKGROUND: Chronic disorders of consciousness are rare clinical conditions that develop after coma and are accompanied by the restoration of wakefulness without the full restoration of consciousness 28 days or longer after brain damage. All women with chronic disorders of consciousness have menstrual irregularities such as oligomenorrhea or secondary amenorrhea. Clinical experience shows that recovery of the menstrual response in a number of patients with chronic disorders of consciousness precedes or is combined with recovery of clear consciousness. The results of a comprehensive examination of patients with different types of ovarian failure, depending on the type of chronic disorders of consciousness, have not been presented in the literature. Therefore, an analysis of the results of such an examination can become the basis for the selection of pathogenetically substantiated hormone-modulating therapy and the development of predictive models for assessing recovery of consciousness from chronic disorders of consciousness. \u0000AIM: The aim of this study was to assess the variants of ovarian failure in patients with different types of chronic disorders of consciousness and to develop prognostic models that evaluate the possibility of restoring consciousness. \u0000MATERIALS AND METHODS: This study enrolled 30 patients aged 18 to 44 years divided into three groups depending on the level of consciousness: vegetative state / unresponsive wakefulness syndrome (n = 12), minimally conscious state minus (n = 6), and minimally conscious state plus (n = 12). Here we examined lipid profile changes (total cholesterol, low-density lipoprotein, high-density lipoprotein) and levels of follicle-stimulating hormone, luteinizing hormone, prolactin, total testosterone, estradiol, free triiodothyronine, thyroxine, thyroid-stimulating hormone, adrenocorticotropic hormone, cortisol, and vitamin 25(OH)D in the blood, as well as levels of melatonin in the blood serum and 6-sulfatoxymelatonin in the urine. In addition, in the blood serum and cerebrospinal fluid, we evaluated levels of brain-derived neurotrophic factor, apoptosis antigen 1, Fas-ligand, glutamate, and S100 protein. We also performed ultrasound of the pelvic organs and mammary glands, magnetic resonance imaging of the chiasmal-sellar region, cervical screening, and genetic study (the prothrombin gene mutation and the Leiden factor). \u0000RESULTS: Hypogonadotropic and normogonadotropic ovarian failures were detected in 36.7% and 63.3% of patients, respectively. No differences in neurotrophin levels between the study groups were found. Three mathematical models were formulated for predicting the recovery of patients into a clear consciousness using the levels of thyroid-stimulating hormone, prolactin, follicle-stimulating hormone, anti-Mllerian hormone, total testosterone, and the age of patients. \u0000CONCLUSIONS: The presented prognostic models by determining hormone levels in the blood plasma allow for calculating the o","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87404581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Premature rupture of membranes occurs in 220% of all pregnancies. The possibility of identifying risk factors and pre dicting this complication are of great interest in practical obstetrics. This review article highlights the current state of the problem and presents approaches to the rational tactics of labor management in case of premature rupture of membranes. We also present the algorithms for the management of labor in the Obstetrics and Gynecology Clinic, Academician I.P. Pavlov First Saint Petersburg State Medical University.
{"title":"Premature rupture of membranes: the current state of the problem and approaches to the rational tactics of labor management","authors":"M. Morozova, V. Bezhenar","doi":"10.17816/jowd202804","DOIUrl":"https://doi.org/10.17816/jowd202804","url":null,"abstract":"Premature rupture of membranes occurs in 220% of all pregnancies. The possibility of identifying risk factors and pre dicting this complication are of great interest in practical obstetrics. This review article highlights the current state of the problem and presents approaches to the rational tactics of labor management in case of premature rupture of membranes. We also present the algorithms for the management of labor in the Obstetrics and Gynecology Clinic, Academician I.P. Pavlov First Saint Petersburg State Medical University.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77745035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fetal growth restriction remains a leading cause of preterm birth and neurological disorders in children and is associated with high neonatal and perinatal morbidity and mortality. Fetal growth restriction is strongly associated with preeclampsia, placental insufficiency, and does not tend to decrease in frequency. The terms small for gestational age and fetal growth restriction are similar in terms of fetometry, however, in modern literature, these concepts differ based on blood flow disorders in the mother-placenta-fetus system and perinatal complications. A deep understanding of the multifactorial pathogenesis of early and late fetal growth restriction will allow for developing targeted therapy of placental insufficiency. Determining the role of new placental growth biomarkers plays a significant role in understanding the pathogenesis of placental dysfunction and developing measures to predict placenta-associated pregnancy complications. This review article highlights new approaches to effective fetal growth restriction screening. Recent advances in ultrasound diagnosis of fetal growth restriction provide the basis for multivariate testing that can provide cost-effective screening for placental-associated pregnancy complications, including fetal growth restriction.
{"title":"Current views on the diagnosis and prognosis of fetal growth restriction (A literature review)","authors":"I. Ignatko, I. Bogomazova, M. A. Kardanova","doi":"10.17816/jowd344442","DOIUrl":"https://doi.org/10.17816/jowd344442","url":null,"abstract":"Fetal growth restriction remains a leading cause of preterm birth and neurological disorders in children and is associated with high neonatal and perinatal morbidity and mortality. Fetal growth restriction is strongly associated with preeclampsia, placental insufficiency, and does not tend to decrease in frequency. The terms small for gestational age and fetal growth restriction are similar in terms of fetometry, however, in modern literature, these concepts differ based on blood flow disorders in the mother-placenta-fetus system and perinatal complications. A deep understanding of the multifactorial pathogenesis of early and late fetal growth restriction will allow for developing targeted therapy of placental insufficiency. Determining the role of new placental growth biomarkers plays a significant role in understanding the pathogenesis of placental dysfunction and developing measures to predict placenta-associated pregnancy complications. \u0000This review article highlights new approaches to effective fetal growth restriction screening. Recent advances in ultrasound diagnosis of fetal growth restriction provide the basis for multivariate testing that can provide cost-effective screening for placental-associated pregnancy complications, including fetal growth restriction.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80301449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article is dedicated to the 25th anniversary of modern history and the 135th anniversary of the first domestic publication of the Journal of Obstetrics and Womens Diseases. The article traces the history of the Journal, its development and state of the art, while reflecting amazing facts, sometimes filled with drama. The Journal of Obstetrics and Womens Diseases has become a prominent publication, thanks to which practitioners, even in the remotest corners of Russia, can be aware of advanced medical technologies and important scientific events, and acquainted with the latest achievements in the field of obstetrics and gynecology, as is evidenced by leading articles published in the Journal.
{"title":"The history of the Journal of Obstetrics and Women’s Diseases (to the 135th anniversary of the first issue and the 25th anniversary of the revived edition)","authors":"Eduard K. Aylamazyan, E. Kira, Igor G. Rodin","doi":"10.17816/jowd340822","DOIUrl":"https://doi.org/10.17816/jowd340822","url":null,"abstract":"This article is dedicated to the 25th anniversary of modern history and the 135th anniversary of the first domestic publication of the Journal of Obstetrics and Womens Diseases. The article traces the history of the Journal, its development and state of the art, while reflecting amazing facts, sometimes filled with drama. The Journal of Obstetrics and Womens Diseases has become a prominent publication, thanks to which practitioners, even in the remotest corners of Russia, can be aware of advanced medical technologies and important scientific events, and acquainted with the latest achievements in the field of obstetrics and gynecology, as is evidenced by leading articles published in the Journal.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78474898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Y. Andreeva, M. Yarmolinskaya, E. V. Misharina, G. Tolibova, V.O. Semenova
BACKGROUND: The prevalence of external genital endometriosis is high, yet there is insufficient understanding of its etiology and pathogenesis. This coupled with the challenges posed by its diagnosis and treatment, and its co-occurrence with type 1 diabetes mellitus, underscores the significance of this issue. AIM: The aim of this study was to evaluate the expression of survivin in the eutopic endometrium, endometriotic lesions, and their concomitant conditions in patients with external genital endometriosis and type 1 diabetes mellitus. MATERIALS AND METHODS: This study enrolled 43 female patients of reproductive age who were divided into four groups. The main group (n = 17) included women with surgically and histologically confirmed external genital endometriosis combined with type 1 diabetes mellitus. The external genital endometriosis group (n = 9) comprised women with isolated external genital endometriosis, while the type 1 diabetes mellitus group (n = 6) included patients with type 1 diabetes mellitus only. Finally, the control group (n = 6) consisted of women without any gynecological pathology. Biological specimens were collected during the follicular phase of the menstrual cycle, and morphological examination was carried out through histological and immunohistochemical evaluation of the endometrium and endometrioid heterotopias. Statistical analysis of the data was performed using the Jamovi software program, with statistical significance defined as p 0.05. RESULTS: Our findings indicate that patients with external genital endometriosis and comorbid type 1 diabetes mellitus exhibit a statistically significant increase in survivin expression in the endometrium compared to the control group or patients with type 1 diabetes mellitus only. However, no significant difference was observed in survivin expression in endometriotiс lesions between patients with external genital endometriosis and those with external genital endometriosis combined with type 1 diabetes mellitus. CONCLUSIONS: The data obtained suggest the role of survivin in the pathogenesis of external genital endometriosis, regardless of the presence of type 1 diabetes mellitus.
{"title":"Evaluation of survivin expression in the endometrium and endometriotic lesions in patients with genital endometriosis, type 1 diabetes mellitus and their comorbidity","authors":"N. Y. Andreeva, M. Yarmolinskaya, E. V. Misharina, G. Tolibova, V.O. Semenova","doi":"10.17816/jowd345406","DOIUrl":"https://doi.org/10.17816/jowd345406","url":null,"abstract":"BACKGROUND: The prevalence of external genital endometriosis is high, yet there is insufficient understanding of its etiology and pathogenesis. This coupled with the challenges posed by its diagnosis and treatment, and its co-occurrence with type 1 diabetes mellitus, underscores the significance of this issue. \u0000AIM: The aim of this study was to evaluate the expression of survivin in the eutopic endometrium, endometriotic lesions, and their concomitant conditions in patients with external genital endometriosis and type 1 diabetes mellitus. \u0000MATERIALS AND METHODS: This study enrolled 43 female patients of reproductive age who were divided into four groups. The main group (n = 17) included women with surgically and histologically confirmed external genital endometriosis combined with type 1 diabetes mellitus. The external genital endometriosis group (n = 9) comprised women with isolated external genital endometriosis, while the type 1 diabetes mellitus group (n = 6) included patients with type 1 diabetes mellitus only. Finally, the control group (n = 6) consisted of women without any gynecological pathology. Biological specimens were collected during the follicular phase of the menstrual cycle, and morphological examination was carried out through histological and immunohistochemical evaluation of the endometrium and endometrioid heterotopias. Statistical analysis of the data was performed using the Jamovi software program, with statistical significance defined as p 0.05. \u0000RESULTS: Our findings indicate that patients with external genital endometriosis and comorbid type 1 diabetes mellitus exhibit a statistically significant increase in survivin expression in the endometrium compared to the control group or patients with type 1 diabetes mellitus only. However, no significant difference was observed in survivin expression in endometriotiс lesions between patients with external genital endometriosis and those with external genital endometriosis combined with type 1 diabetes mellitus. \u0000CONCLUSIONS: The data obtained suggest the role of survivin in the pathogenesis of external genital endometriosis, regardless of the presence of type 1 diabetes mellitus.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79778044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Yarmolinskaya, A. A. Tsypurdeeva, Nikolai I. Polenov, O. Malysheva, A. S. Koltsova, A. Pendina, O. Efimova, N. Osinovskaya, N. Shved
We present a clinical case of giant uterine fibroids in this research, with the peculiarity of surgical treatment and the course of the postoperative period described. A series of genetic studies specific to uterine fibroids was performed, namely, mutations in the MED12 gene, overexpression of the HMGA2 gene, and chromosomal imbalance. We did not detect mutations of exon 2 of the MED12 gene and an increase in the expression of the HMGA2 gene in the patients myomatous node sample. The molecular karyotype arr(22)1 (chromosome 22 monosomy) was established by comparative genomic hybridization in the tissue of giant uterine fibroids.
{"title":"Giant uterine fibroids with chromosome 22 monosomy. A clinical case","authors":"M. Yarmolinskaya, A. A. Tsypurdeeva, Nikolai I. Polenov, O. Malysheva, A. S. Koltsova, A. Pendina, O. Efimova, N. Osinovskaya, N. Shved","doi":"10.17816/jowd375307","DOIUrl":"https://doi.org/10.17816/jowd375307","url":null,"abstract":"We present a clinical case of giant uterine fibroids in this research, with the peculiarity of surgical treatment and the course of the postoperative period described. A series of genetic studies specific to uterine fibroids was performed, namely, mutations in the MED12 gene, overexpression of the HMGA2 gene, and chromosomal imbalance. We did not detect mutations of exon 2 of the MED12 gene and an increase in the expression of the HMGA2 gene in the patients myomatous node sample. The molecular karyotype arr(22)1 (chromosome 22 monosomy) was established by comparative genomic hybridization in the tissue of giant uterine fibroids.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86809928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: The prevalence of gestational diabetes mellitus has increased significantly and has become a global health problem, affecting 9.325.5% of pregnant women worldwide. Violation of the interaction of various body systems with the intestinal microbiota can be the cause of the development of insulin resistance. The study of the state of the intestinal microbiota based on the results of the study of the species composition of microorganisms in feces by the polymerase chain reaction method is necessary to understand the mechanisms of gestational diabetes mellitus development. AIM: The aim of this study was to evaluate the intestinal microbiota status in women with normal pregnancy and pregnancy complicated by gestational diabetes mellitus. MATERIALS AND METHODS: We examined 51 pregnant women in the period 2020-2022. The average age of women with normal pregnancy (n = 20) and pregnancy complicated by gestational diabetes mellitus (n = 31) was 29 (27.0; 32.5) and 31 (27.0; 35.0) years, respectively. The intestinal microbiota status was assessed based on the microbial species composition in feces using real-time polymerase chain reaction. All women underwent a test for carbohydrate metabolism at various gestation periods. RESULTS: We have established a positive relation between Bacteroides thetaiotaomicron and Body Mass Index before pregnancy (r = 0.42). The number of Bacteroides thetaiotaomicron in the 1st, 2nd and 3rd trimesters of gestation positively correlated with the initial weight of women before pregnancy (r = 0.60, r = 0.52, r = 0.47, respectively; p 0.05). The Bacteroides spp. / Faecalibacterium prausnitzii ratio in women with gestational diabetes mellitus was negatively correlated with the average blood glucose level in the 3rd trimester of pregnancy (r = 0.81; p 0.05). Parvimonas micra positively correlated with venous plasma glucose levels in the presence of gestational diabetes mellitus (r = 0.58; p 0.05). A positive relationship was obtained between the number of Escherichia coli in pregnant women in the 1st trimester and the average glucose level in the 3rd trimester of pregnancy (r = 0.41; p 0.05). It was demonstrated that the growth of Bacteroides fragilis in the large intestine of pregnant women with gestational diabetes mellitus in the 3rd trimester of pregnancy correlated with subnormal blood glucose levels (r = 0.77; p 0.05), which may be due to a diet disorder (insufficient carbohydrate intake) or insulin overdose, which can lead to hypoglycemic conditions. In the group of women with gestational diabetes mellitus, a positive correlation was obtained between glycated hemoglobin level and the opportunistic pathogen Klebsiella pneumoniae representative amount in the 1st trimester of pregnancy (r = 0.46; p 0.05). In addition, we have found positive relations between the Citrobacter spp. / Enterobacter spp. ratio and the maximum blood glucose level in women with gestational diabetes mellitus in the 1st, 2nd and 3rd trimeste
{"title":"Carbohydrate metabolism and the species composition of the intestinal microbiota in women with gestational diabetes mellitus","authors":"T. A. Zinina, A. Tiselko, M. Yarmolinskaya","doi":"10.17816/jowd321748","DOIUrl":"https://doi.org/10.17816/jowd321748","url":null,"abstract":"BACKGROUND: The prevalence of gestational diabetes mellitus has increased significantly and has become a global health problem, affecting 9.325.5% of pregnant women worldwide. Violation of the interaction of various body systems with the intestinal microbiota can be the cause of the development of insulin resistance. The study of the state of the intestinal microbiota based on the results of the study of the species composition of microorganisms in feces by the polymerase chain reaction method is necessary to understand the mechanisms of gestational diabetes mellitus development. \u0000AIM: The aim of this study was to evaluate the intestinal microbiota status in women with normal pregnancy and pregnancy complicated by gestational diabetes mellitus. \u0000MATERIALS AND METHODS: We examined 51 pregnant women in the period 2020-2022. The average age of women with normal pregnancy (n = 20) and pregnancy complicated by gestational diabetes mellitus (n = 31) was 29 (27.0; 32.5) and 31 (27.0; 35.0) years, respectively. The intestinal microbiota status was assessed based on the microbial species composition in feces using real-time polymerase chain reaction. All women underwent a test for carbohydrate metabolism at various gestation periods. \u0000RESULTS: We have established a positive relation between Bacteroides thetaiotaomicron and Body Mass Index before pregnancy (r = 0.42). The number of Bacteroides thetaiotaomicron in the 1st, 2nd and 3rd trimesters of gestation positively correlated with the initial weight of women before pregnancy (r = 0.60, r = 0.52, r = 0.47, respectively; p 0.05). The Bacteroides spp. / Faecalibacterium prausnitzii ratio in women with gestational diabetes mellitus was negatively correlated with the average blood glucose level in the 3rd trimester of pregnancy (r = 0.81; p 0.05). Parvimonas micra positively correlated with venous plasma glucose levels in the presence of gestational diabetes mellitus (r = 0.58; p 0.05). A positive relationship was obtained between the number of Escherichia coli in pregnant women in the 1st trimester and the average glucose level in the 3rd trimester of pregnancy (r = 0.41; p 0.05). It was demonstrated that the growth of Bacteroides fragilis in the large intestine of pregnant women with gestational diabetes mellitus in the 3rd trimester of pregnancy correlated with subnormal blood glucose levels (r = 0.77; p 0.05), which may be due to a diet disorder (insufficient carbohydrate intake) or insulin overdose, which can lead to hypoglycemic conditions. In the group of women with gestational diabetes mellitus, a positive correlation was obtained between glycated hemoglobin level and the opportunistic pathogen Klebsiella pneumoniae representative amount in the 1st trimester of pregnancy (r = 0.46; p 0.05). In addition, we have found positive relations between the Citrobacter spp. / Enterobacter spp. ratio and the maximum blood glucose level in women with gestational diabetes mellitus in the 1st, 2nd and 3rd trimeste","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84867336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Bespalova, Margarita O. Shengelia, V. A. Zagaynova, S. Chepanov, E. Komarova, I. Kogan
BACKGROUND: One of the most important research areas in the field of reproductive medicine is the search for biochemical and immunological parameters of oocyte quality and predicting the effectiveness of assisted reproductive technology protocols. AIM: The aim of this study was to evaluate the expression of follicular fluid soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin levels in women with reproductive disorders. MATERIALS AND METHODS: This prospective cohort study included 22 patients undergoing infertility treatment in a superovulation stimulation protocol using gonadotropin-releasing hormone antagonists. The inclusion criteria were age from 25 to 39 years, tubal-peritoneal factor infertility, and voluntary participation informed consent. The levels of soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin in follicular fluid samples were determined on the day of transvaginal follicle puncture by enzyme immunoassay. RESULTS: We established an inverse correlation between the expression levels of progesterone-inducing blocking factor and relaxin (r = 0.450) in the follicular fluid, antibodies to thyroperoxidase (r = 0.649), and thyroid-stimulating hormone (r = 0.519). We also found a direct correlation between human leukocyte antigen-E parameters in the follicular fluid, age (r = 0.813) and Body Mass Index (r = 0.866), as well as between human leukocyte antigen-C expression levels and total testosterone (r = 0.960). No data were obtained on any significant correlations between the studied biomarkers and the number of received oocytes. CONCLUSIONS: In this comprehensive study, we were the first who found the expression levels of five different follicular fluid components, namely, soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin. Such a complex assessment of the follicular fluid can allow for establishing the quality of the oocyte to predict the onset of pregnancy in an in vitro fertilization protocol.
{"title":"Biological profile of the follicular fluid. A pilot study","authors":"O. Bespalova, Margarita O. Shengelia, V. A. Zagaynova, S. Chepanov, E. Komarova, I. Kogan","doi":"10.17816/jowd112564","DOIUrl":"https://doi.org/10.17816/jowd112564","url":null,"abstract":"BACKGROUND: One of the most important research areas in the field of reproductive medicine is the search for biochemical and immunological parameters of oocyte quality and predicting the effectiveness of assisted reproductive technology protocols. \u0000AIM: The aim of this study was to evaluate the expression of follicular fluid soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin levels in women with reproductive disorders. \u0000MATERIALS AND METHODS: This prospective cohort study included 22 patients undergoing infertility treatment in a superovulation stimulation protocol using gonadotropin-releasing hormone antagonists. The inclusion criteria were age from 25 to 39 years, tubal-peritoneal factor infertility, and voluntary participation informed consent. The levels of soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin in follicular fluid samples were determined on the day of transvaginal follicle puncture by enzyme immunoassay. \u0000RESULTS: We established an inverse correlation between the expression levels of progesterone-inducing blocking factor and relaxin (r = 0.450) in the follicular fluid, antibodies to thyroperoxidase (r = 0.649), and thyroid-stimulating hormone (r = 0.519). We also found a direct correlation between human leukocyte antigen-E parameters in the follicular fluid, age (r = 0.813) and Body Mass Index (r = 0.866), as well as between human leukocyte antigen-C expression levels and total testosterone (r = 0.960). No data were obtained on any significant correlations between the studied biomarkers and the number of received oocytes. \u0000CONCLUSIONS: In this comprehensive study, we were the first who found the expression levels of five different follicular fluid components, namely, soluble human leukocyte antigen-G, human leukocyte antigen-E, human leukocyte antigen-C, progesterone-inducing blocking factor, and relaxin. Such a complex assessment of the follicular fluid can allow for establishing the quality of the oocyte to predict the onset of pregnancy in an in vitro fertilization protocol.","PeriodicalId":16623,"journal":{"name":"Journal of obstetrics and women's diseases","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88618259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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