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Influence of a die spacer on the fit and load to failure of cantilever resin-bonded fixed dental prostheses. 模具垫片对悬臂树脂粘接固定义齿配合和载荷失效的影响。
IF 1.7 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-10-16 Epub Date: 2025-09-10 DOI: 10.2334/josnusd.25-0048
Bing X Tan, Timothy Wong, Qicheng Wu, Christine Tran, Vera Wang, Wei C Tan, Louisa Taylor, Ranjana Varma, Abanoub K Tawfik, Dimitrios Tomazos, Ali Wassouf, Coco Hs Teng, Joseph Palamara, Jaafar Abduo

Purpose: To evaluate the effect of die spacer thickness on the fit and load to failure of cantilever resin-bonded fixed dental prostheses (RBFDPs).

Methods: Two identical maxillary RBFDPs with a retainer on the canine were designed to replace a lateral incisor. One design involved a closely fitting retainer with no die spacer (NDS), and the other included an 80-µm die spacer (DS). A total of 10 RBFDPs were produced for each group by selective laser melting. To simulate a natural canine abutment, lithium disilicate abutments were fabricated digitally. After bonding the RBFDP with an adhesive resin luting agent, the marginal fit was measured, and the specimens were then loaded until failure of the luting agent.

Results: The mean marginal fit in the NDS and DS groups was 79.1 µm and 75.7 µm, respectively, and the corresponding mean maximum load to failure was 272.8 N and 254.7 N, respectively. The inter-group differences in these values were not significant.

Conclusion: The inclusion of a RBFDP die spacer had no effect on the marginal fit and maximum load to failure. In addition, the die spacer had no significant effect on the pattern of resin luting agent failure.

目的:探讨模具垫片厚度对悬臂树脂粘接固定义齿(rbfdp)配合和失效载荷的影响。方法:设计两个相同的上颌rbfdp,在犬齿上带固位器代替侧切牙。一种设计包括一个紧密配合的保持器,没有模具垫片(NDS),另一个包括一个80微米的模具垫片(DS)。每组采用选择性激光熔融法制备10个rbfdp。为了模拟天然的犬牙基牙,二硅酸锂基牙被数字化制作。将RBFDP与粘接树脂粘结剂粘合后,测量边缘配合度,然后加载试件直至粘结剂失效。结果:NDS组和DS组的平均边际拟合分别为79.1µm和75.7µm,相应的平均最大失效载荷分别为272.8 N和254.7 N。各组间差异均不显著。结论:RBFDP模具垫片的加入对边缘配合和最大失效负荷没有影响。此外,模具间隔对树脂润滑剂失效模式无显著影响。
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引用次数: 0
Effect of precipitate formation in the root canal on the accuracy of electronic apex locators. 根管内沉淀对电子根尖定位器精度的影响。
IF 1.7 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-10-16 Epub Date: 2025-09-10 DOI: 10.2334/josnusd.25-0079
Kürşat Er, Simay Koç, Damla Erkal, Dide Tekinarslan, Ömer Kesmez, Feride Demir, Eszther Borbely

Purpose: This study evaluated how the formation of various types of precipitates affects the accuracy of electronic apex locators in determining the working length.

Methods: Two hundred and forty extracted human maxillary incisors were selected. A total of eight groups were used: four groups (n = 30) for the application of different irrigants; saline, sodium hypochlorite (5.25% NaOCl), chlorhexidine (2% CHX) and ethylenediaminetetraacetic acid (EDTA) (17% EDTA), and four groups (n = 30) for the formation of precipitates; orange-brown (2% CHX + 5.25% NaOCl), orange-white (2% CHX + 0.16% NaOCl), white milky (2% CHX + 17% EDTA), salt (2% CHX + saline). Electronic working length was determined by two different electronic apex locators.

Results: The success rate of the 5.25% NaOCl group was significantly higher than that of the white milky precipitate group. The electronic apex locators were highly effective in identifying working length, but the success rate of both devices declined due to the production of a white milky precipitate.

Conclusion: The study emphasizes the importance of selecting appropriate irrigation protocols for optimal treatment outcomes, as the chemical interactions between solutions and precipitates significantly influence electronic apex locator measurements, highlighting the need for careful consideration of irrigant compatibility.

目的:评价不同类型沉淀的形成对电子顶点定位仪确定工作长度精度的影响。方法:选择拔除的人上颌切牙240颗。共采用8组:4组(n = 30)分别应用不同的灌洗剂;生理盐水,次氯酸钠(5.25% NaOCl),氯己定(2% CHX)和乙二胺四乙酸(EDTA) (17% EDTA),四组(n = 30)形成沉淀;橙棕色(2%CHX + 5.25% NaOCl),橙白色(2%CHX + 0.16% NaOCl),白色乳白色(2%CHX + 17% EDTA),盐(2%CHX +生理盐水)。电子工作长度由两种不同的电子顶点定位器确定。结果:5.25% NaOCl组的成功率明显高于白色乳状沉淀物组。电子顶点定位器在识别工作长度方面非常有效,但由于产生白色乳状沉淀物,两种装置的成功率都有所下降。结论:该研究强调了选择合适的灌溉方案以获得最佳处理结果的重要性,因为溶液和沉淀物之间的化学相互作用显著影响电子顶点定位器的测量,强调了仔细考虑灌溉兼容性的必要性。
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引用次数: 0
Effects of sintering protocols, yttria content, and zirconia thickness on the optical properties of monolithic zirconia. 烧结工艺、氧化钇含量和氧化锆厚度对单片氧化锆光学性能的影响。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-07-16 Epub Date: 2025-05-28 DOI: 10.2334/josnusd.25-0083
Shoko Miura, Shohei Tsukada, Takafumi Fujita, Masanori Fujisawa, Pekka Vallittu, Lippo Lassila

Purpose: This laboratory-based study evaluated the effects of sintering protocols, yttria content, and zirconia thickness on the optical properties of monolithic zirconia.

Methods: Three partially stabilized zirconia (PSZ) materials, one monolayer (HT) and two multilayer (GE, FX) samples, with thicknesses of 0.5-1.5 mm were tested under conventional and speed sintering protocols. Translucency parameter (TP), color difference (ΔE00), and spectral reflectance were measured using a spectrophotometer against standardized black and white backgrounds. Statistical analyses were performed using a one-way analysis of variance, Tukey's post-hoc tests, and correlation analysis.

Results: The TP values decreased with increasing zirconia thickness; significant differences were observed between the sintering protocols. Speed sintering resulted in lower transparency in certain materials, particularly GE. Conversely, HT exhibited consistent optical properties across the sintering protocols. The ΔE00 values for multilayer zirconia exceeded clinically acceptable thresholds, with greater variations observed for thicker specimens. Spectral reflectance curves were minimally influenced by the sintering protocol and thickness for monolayer zirconia, whereas they showed significant variations for multilayer zirconia.

Conclusion: The results indicate that sintering protocols and material composition significantly influence the optical properties of zirconia, underscoring the necessity of optimizing processing conditions to enhance both esthetic and functional performance in clinical applications.

目的:研究烧结工艺、氧化钇含量和氧化锆厚度对单片氧化锆光学性能的影响。方法:在常规烧结和快速烧结条件下,对三种部分稳定的氧化锆(PSZ)材料,1种单层(HT)和2种多层(GE, FX)样品进行了厚度为0.5 ~ 1.5 mm的测试。半透明参数(TP)、色差(ΔE00)和光谱反射率使用分光光度计在标准的黑白背景下测量。统计分析采用单向方差分析、Tukey事后检验和相关分析。结果:TP值随氧化锆厚度的增加而降低;烧结工艺之间存在显著差异。快速烧结导致某些材料的透明度降低,特别是GE。相反,HT在烧结过程中表现出一致的光学性质。多层氧化锆的ΔE00值超过了临床可接受的阈值,在较厚的标本中观察到更大的变化。光谱反射率曲线受单层氧化锆烧结工艺和厚度的影响最小,而多层氧化锆的光谱反射率曲线变化显著。结论:烧结工艺和材料组成对氧化锆的光学性能有显著影响,强调了优化工艺条件以提高临床应用中美观和功能性能的必要性。
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引用次数: 0
Efficacy evaluation with stratified randomized controlled study of foam dentifrice containing isodecyl galactosides in older adults. 用分层随机对照研究评价含异癸基半乳糖泡沫牙膏对老年人的疗效。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-07-16 Epub Date: 2025-06-15 DOI: 10.2334/josnusd.24-0387
Yuki Iwawaki, Takaharu Goto, Takahiro Kishimoto, Takashi Matsuda, Keiko Fujimoto, Kan Nagao, Kenji Yoshimura, Teppei Tajikara, Yoshiyuki Eshita, Yoshitaka Yano, Tetsuo Ichikawa

Purpose: Oral hygiene is mainly maintained by mechanical plaque removal, but this is often inadequate in older adults. Isodecyl galactosides (ID-Gal) inhibits bacterial co-aggregation and could promote the maintenance and improvement of oral hygiene. In this study, the efficacy of ID-Gal-containing foam dentifrice in oral hygiene among older adults was evaluated.

Methods: Fifty-six subjects aged 65 years or older visited Tokushima University Hospital and were randomly assigned to interventions using ID-Gal-containing foam dentifrice applied to the dorsum of the tongue, or to controls using their usual dentifrice. Microbiological and clinical oral health parameters were evaluated before, and at 1 and 2 months after the intervention.

Results: The intervention group showed significant decreases in black pigment-producing bacteria on the dorsum of the tongue, Fusobacterium, and total bacterial counts on the mucosa below the denture base after 1 month. Additionally, the intervention group showed significant decrease in the Winkle Tongue Coating Index (WTCI) after 2 months.

Conclusion: ID-Gal-containing foam dentifrice decreased the number of bacteria by suppressing plaque formation and contributed to a reduction in the number of bacteria on the tongue and the WTCI. Collectively, these results suggest that ID-Gal-containing foam dentifrice is effective in maintaining and improving oral hygiene.

目的:口腔卫生主要通过机械清除菌斑来维持,但这在老年人中往往是不够的。异癸基半乳糖苷(Isodecyl galtosides, ID-Gal)能抑制细菌共聚集,促进口腔卫生的维持和改善。本研究评估含id - gal泡沫牙膏在老年人口腔卫生中的功效。方法:56名年龄在65岁及以上的患者到德岛大学医院就诊,随机分配到使用含id - gal泡沫牙膏涂抹舌背的干预组和使用常规牙膏的对照组。在干预前、干预后1个月和2个月分别评估微生物学和临床口腔健康参数。结果:干预组1个月后舌背黑色色素生成菌、梭杆菌及义齿基托下黏膜细菌总数明显减少。干预组2个月后Winkle Tongue Coating Index (WTCI)明显下降。结论:含id - gal泡沫牙膏通过抑制牙菌斑的形成来减少细菌数量,有助于减少舌部和WTCI上的细菌数量。总之,这些结果表明含id - gal泡沫牙膏在维持和改善口腔卫生方面是有效的。
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引用次数: 0
Usefulness of an occlusal force device incorporating a microcomputer. 结合微型计算机的咬合力装置的实用性。
IF 1.7 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-07-16 Epub Date: 2025-05-23 DOI: 10.2334/josnusd.25-0046
Hiroshi Shiga, Kunihisa Nakajima, Marie Komino, Hanako Uesugi, Masaoki Yokoyama, Yuka I Sumita

This study was conducted to clarify the usefulness of a newly developed occlusal force device. An arbitrary load was applied to the device 100 times, and the measured values were recorded. The loading and measured values were extremely close, and the error rate was fairly small. There was a close relationship between the occlusal force measured with a device based on the dental prescale and that measured using the new device. These results suggest that the newly developed device can measure and evaluate occlusal force with a degree of accuracy equivalent to the dental prescale.

本研究是为了阐明一种新开发的咬合力装置的实用性。对该装置施加任意负载100次,并记录测量值。加载值与实测值非常接近,误差率很小。使用基于牙预分尺的设备测量的咬合力与使用新设备测量的咬合力之间存在密切的关系。这些结果表明,新开发的装置可以测量和评估咬合力,其精度相当于牙齿预分尺。
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引用次数: 0
Clinical success of vital pulp therapy for pulp-exposed permanent teeth: a systematic review and meta-analysis. 关键牙髓治疗牙髓外露恒牙的临床成功:一项系统回顾和荟萃分析。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-07-16 Epub Date: 2025-06-15 DOI: 10.2334/josnusd.24-0452
Natthanon Pusa, Pattama Chailertvanitkul, Suttichai Krisanaprakornkit, Chetta Ngamjarus, Nutthapong Kantrong, Thalerngsak Samaksamarn

Purpose: To compare the clinical success of different forms of vital pulp therapy using various pulp-capping materials.

Methods: Two independent researchers selected relevant articles from databases, extracted data, evaluated bias risk, and performed meta-analysis. Among 337 studies retrieved, 21 with a total of 1,816 participants and 1,948 treated permanent teeth were eligible and selected for 17 types of comparison. Meta-analysis was conducted for only six of these 17 comparisons.

Results: Among the six comparisons, the success of direct pulp capping with mineral trioxide aggregate (MTA) was significantly higher than that using calcium hydroxide (relative risk = 1.21, 95% confidence interval = 1.02-1.42, P = 0.03). There was no significant difference in the success of direct pulp capping between mineral trioxide aggregate and biodentine, nor between partial pulpotomy and full pulpotomy for either type of pulp capping material.

Conclusion: The use of MTA for direct pulp capping appears to yield significantly better clinical success than the use of calcium hydroxide.

目的:比较不同盖髓材料对不同形式的生命髓治疗的临床效果。方法:两名独立研究人员从数据库中选择相关文章,提取数据,评估偏倚风险,进行meta分析。在检索到的337项研究中,21项共1816名参与者和1948颗治疗过的恒牙符合条件,并被选中进行17种类型的比较。这17个比较中只有6个进行了荟萃分析。结果:6个对比中,三氧化二矿骨料(MTA)直接盖髓成功率显著高于氢氧化钙(相对风险= 1.21,95%可信区间= 1.02 ~ 1.42,P = 0.03)。三氧化矿物骨料和生物牙汀直接盖髓成功率无显著差异,两种盖髓材料的部分切髓和完全切髓成功率也无显著差异。结论:MTA直接盖髓效果明显优于氢氧化钙盖髓效果。
{"title":"Clinical success of vital pulp therapy for pulp-exposed permanent teeth: a systematic review and meta-analysis.","authors":"Natthanon Pusa, Pattama Chailertvanitkul, Suttichai Krisanaprakornkit, Chetta Ngamjarus, Nutthapong Kantrong, Thalerngsak Samaksamarn","doi":"10.2334/josnusd.24-0452","DOIUrl":"10.2334/josnusd.24-0452","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the clinical success of different forms of vital pulp therapy using various pulp-capping materials.</p><p><strong>Methods: </strong>Two independent researchers selected relevant articles from databases, extracted data, evaluated bias risk, and performed meta-analysis. Among 337 studies retrieved, 21 with a total of 1,816 participants and 1,948 treated permanent teeth were eligible and selected for 17 types of comparison. Meta-analysis was conducted for only six of these 17 comparisons.</p><p><strong>Results: </strong>Among the six comparisons, the success of direct pulp capping with mineral trioxide aggregate (MTA) was significantly higher than that using calcium hydroxide (relative risk = 1.21, 95% confidence interval = 1.02-1.42, P = 0.03). There was no significant difference in the success of direct pulp capping between mineral trioxide aggregate and biodentine, nor between partial pulpotomy and full pulpotomy for either type of pulp capping material.</p><p><strong>Conclusion: </strong>The use of MTA for direct pulp capping appears to yield significantly better clinical success than the use of calcium hydroxide.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":" ","pages":"107-115"},"PeriodicalIF":1.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between severity of periodontitis and oral health-related quality of life in Korean adults. 韩国成年人牙周炎严重程度与口腔健康相关生活质量的关系
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-07-16 Epub Date: 2025-04-28 DOI: 10.2334/josnusd.25-0040
Eun-Young Kwon, Ji-Young Joo

Purpose: This study investigated the relationship between periodontitis severity and oral health-related quality of life (OHRQoL) among Korean adults.

Methods: This retrospective study included 291 adults aged 20-59. Age, sex, dental visits during the past year, frequency of tooth brushing, number of teeth, and dental caries were designated as confounders, and their information was collected. The severity of periodontitis was dichotomized into no/mild and moderate/severe. OHRQoL was evaluated using the Korean version of the 14-item oral health impact profile (OHIP-14K). Logistic and negative binomial regression analyses were used to calculate odds ratios (OR) or incidence rate ratios (IRR) with 95% confidence intervals (CI).

Results: The prevalence, severity, and extent of oral impact according to total OHIP-14K score were significantly higher in patients with moderate/severe periodontitis than in those with no/mild periodontitis (P < 0.05). The adjusted regression model indicated that age, sex, number of teeth and severity of periodontitis (OR = 2.22, 95% CI = 1.23-3.99) were significantly associated with the prevalence of impacts. Regarding the severity of impacts, sex, dental visits, and severity of periodontitis (IRR = 1.44, 95% CI = 1.16-1.79) were significantly associated with OHRQoL. Regarding the extent of impacts, sex, and severity of periodontitis (IRR = 1.85, 95% CI = 1.21-2.84) were significantly related to OHRQoL.

Conclusion: The severity of periodontitis is inversely related to the OHRQoL in Korean adults.

目的:本研究探讨韩国成人牙周炎严重程度与口腔健康相关生活质量(OHRQoL)的关系。方法:对291名年龄在20 ~ 59岁的成年人进行回顾性研究。年龄、性别、过去一年的牙科就诊次数、刷牙频率、牙齿数量和龋齿被指定为混杂因素,并收集他们的信息。牙周炎的严重程度分为无/轻度和中度/重度。使用韩国版的14项口腔健康影响量表(OHIP-14K)评估OHRQoL。采用Logistic和负二项回归分析计算95%置信区间(CI)的比值比(OR)或发病率比(IRR)。结果:中重度牙周炎患者OHIP-14K总分的患病率、严重程度及口腔影响程度均显著高于无轻度牙周炎患者(P < 0.05)。调整后的回归模型显示,年龄、性别、牙齿数量和牙周炎严重程度(OR = 2.22, 95% CI = 1.23-3.99)与影响发生率显著相关。对于影响的严重程度,性别、牙科就诊和牙周炎的严重程度(IRR = 1.44, 95% CI = 1.16-1.79)与OHRQoL显著相关。至于影响程度,性别和牙周炎的严重程度(IRR = 1.85, 95% CI = 1.21-2.84)与OHRQoL显著相关。结论:韩国成人牙周炎的严重程度与OHRQoL呈负相关。
{"title":"Relationship between severity of periodontitis and oral health-related quality of life in Korean adults.","authors":"Eun-Young Kwon, Ji-Young Joo","doi":"10.2334/josnusd.25-0040","DOIUrl":"10.2334/josnusd.25-0040","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated the relationship between periodontitis severity and oral health-related quality of life (OHRQoL) among Korean adults.</p><p><strong>Methods: </strong>This retrospective study included 291 adults aged 20-59. Age, sex, dental visits during the past year, frequency of tooth brushing, number of teeth, and dental caries were designated as confounders, and their information was collected. The severity of periodontitis was dichotomized into no/mild and moderate/severe. OHRQoL was evaluated using the Korean version of the 14-item oral health impact profile (OHIP-14K). Logistic and negative binomial regression analyses were used to calculate odds ratios (OR) or incidence rate ratios (IRR) with 95% confidence intervals (CI).</p><p><strong>Results: </strong>The prevalence, severity, and extent of oral impact according to total OHIP-14K score were significantly higher in patients with moderate/severe periodontitis than in those with no/mild periodontitis (P < 0.05). The adjusted regression model indicated that age, sex, number of teeth and severity of periodontitis (OR = 2.22, 95% CI = 1.23-3.99) were significantly associated with the prevalence of impacts. Regarding the severity of impacts, sex, dental visits, and severity of periodontitis (IRR = 1.44, 95% CI = 1.16-1.79) were significantly associated with OHRQoL. Regarding the extent of impacts, sex, and severity of periodontitis (IRR = 1.85, 95% CI = 1.21-2.84) were significantly related to OHRQoL.</p><p><strong>Conclusion: </strong>The severity of periodontitis is inversely related to the OHRQoL in Korean adults.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":" ","pages":"116-121"},"PeriodicalIF":1.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between vertical hyoid bone position and swallowing function in older adults. 老年人舌骨垂直位置与吞咽功能的关系。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-04-16 Epub Date: 2025-03-12 DOI: 10.2334/josnusd.24-0409
Yuuri Oku, Hideki Suito, Keiko Fujimoto, Adityakrisna Yoshi Putra Wigianto, Tetsuo Ichikawa, Kan Nagao

Purpose: The purpose of this study was to investigate swallowing function of older adults with lowered hyoid bone position.

Methods: A total of 60 older adults (23 males and 37 females, mean age: 70.1 years) with no diagnosed dysphagia participated in the study. Participants were divided into two groups (High and Low group) based on the anterior point of the hyoid bone (H) with respect to the line connecting the most inferior and anterior point on the corpus of the third cervical vertebra (C3) and Menton (Me). Swallowing function was measured using the Repetitive Saliva Swallow Test (RSST), the Eating Assessment Tool (EAT-10), jaw-opening force (JOF) and tongue pressure (TP). Each group was studied for its relationship to swallowing function. Additionally, the RSST of males and females in the Low group were compared.

Results: Males in the Low group had a significantly higher JOF (P = 0.009) in the RSST ≥ 5 group than in the RSST < 5 group. Females in the Low group had no measurements that showed significant differences between the RSST ≥ 4 and RSST < 4 groups.

Conclusion: JOF may affect swallowing function in older males with lowered hyoid bone position.

目的:本研究的目的是探讨舌骨位置降低的老年人吞咽功能。方法:共60例未确诊吞咽困难的老年人(男性23例,女性37例,平均年龄70.1岁)参与研究。参与者根据舌骨前点(H)相对于连接第三颈椎(C3)和Menton (Me)的最下方和前点的线分为两组(高组和低组)。采用重复唾液吞咽试验(RSST)、进食评估工具(EAT-10)、开颌力(JOF)和舌压(TP)测量吞咽功能。研究各组与吞咽功能的关系。并比较Low组男女RSST。结果:低组男性的JOF在RSST≥5组显著高于RSST < 5组(P = 0.009)。Low组女性RSST≥4组和RSST < 4组间无显著差异。结论:JOF可能影响舌骨位置降低的老年男性吞咽功能。
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引用次数: 0
Decalcification of calcified tissues induced by inorganic polyphosphate in chondrogenic ATDC5 cells in the presence of insulin. 胰岛素作用下,ATDC5软骨细胞中无机多磷酸盐诱导的钙化组织脱钙。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-04-16 Epub Date: 2025-03-08 DOI: 10.2334/josnusd.24-0429
Tatsuaki Fujiki, Hiroshi Shiratsuchi, Yoshikazu Mikami, Taku Toriumi, Yoshiyuki Yonehara, Hiromasa Tsuda

Purpose: Inorganic polyphosphate (PolyP), a polymer of orthophosphate, strongly promotes mineralized tissue formation. This study explored the conditions necessary for PolyP to induce calcified deposits in cartilage and assessed the role of insulin in modulating PolyP-induced tissue calcification.

Methods: Murine chondrogenic ATDC5 cells were cultured under growth, mineralization, or PolyP-induced calcification conditions, with or without insulin. Calcified nodules were stained with Alizarin Red S, and conditioned media were analyzed for pH and lactate concentration using a pH meter and a lactate assay kit-WST.

Results: PolyP treatment of ATDC5 cells led to calcified deposits by day 5, both with and without insulin. However, in the presence of insulin, these deposits were nearly fully decalcified by day 14. Conditioned media with insulin had a lower pH and a higher lactate concentration compared to those without insulin, with lactate levels sufficient to demineralize the PolyP-induced calcified deposits.

Conclusion: These data suggest that treatment of ATDC5 chondrogenic cells with PolyP accelerates the formation of mineralized tissue. However, PolyP-induced calcified nodules undergo demineralization owing to lactate production by cells in the presence of insulin.

用途:无机聚磷酸盐(PolyP)是一种正磷酸盐的聚合物,能强烈促进矿化组织的形成。本研究探讨了息肉诱导软骨钙化沉积的必要条件,并评估了胰岛素在调节息肉诱导的组织钙化中的作用。方法:小鼠软骨细胞ATDC5在生长、矿化或息肉诱导的钙化条件下培养,有或没有胰岛素。用茜素红S染色钙化结节,用pH计和乳酸测定试剂盒wst分析条件培养基的pH和乳酸浓度。结果:ATDC5细胞的息肉处理在第5天导致钙化沉积,无论是否使用胰岛素。然而,在胰岛素的作用下,这些沉积物在第14天几乎完全脱钙。与没有胰岛素的条件培养基相比,有胰岛素的条件培养基具有较低的pH值和较高的乳酸浓度,乳酸水平足以使息肉诱导的钙化沉积物脱矿。结论:这些数据表明,用PolyP治疗ATDC5软骨细胞可加速矿化组织的形成。然而,息肉诱导的钙化结节在胰岛素的作用下由于细胞产生乳酸而发生脱矿。
{"title":"Decalcification of calcified tissues induced by inorganic polyphosphate in chondrogenic ATDC5 cells in the presence of insulin.","authors":"Tatsuaki Fujiki, Hiroshi Shiratsuchi, Yoshikazu Mikami, Taku Toriumi, Yoshiyuki Yonehara, Hiromasa Tsuda","doi":"10.2334/josnusd.24-0429","DOIUrl":"10.2334/josnusd.24-0429","url":null,"abstract":"<p><strong>Purpose: </strong>Inorganic polyphosphate (PolyP), a polymer of orthophosphate, strongly promotes mineralized tissue formation. This study explored the conditions necessary for PolyP to induce calcified deposits in cartilage and assessed the role of insulin in modulating PolyP-induced tissue calcification.</p><p><strong>Methods: </strong>Murine chondrogenic ATDC5 cells were cultured under growth, mineralization, or PolyP-induced calcification conditions, with or without insulin. Calcified nodules were stained with Alizarin Red S, and conditioned media were analyzed for pH and lactate concentration using a pH meter and a lactate assay kit-WST.</p><p><strong>Results: </strong>PolyP treatment of ATDC5 cells led to calcified deposits by day 5, both with and without insulin. However, in the presence of insulin, these deposits were nearly fully decalcified by day 14. Conditioned media with insulin had a lower pH and a higher lactate concentration compared to those without insulin, with lactate levels sufficient to demineralize the PolyP-induced calcified deposits.</p><p><strong>Conclusion: </strong>These data suggest that treatment of ATDC5 chondrogenic cells with PolyP accelerates the formation of mineralized tissue. However, PolyP-induced calcified nodules undergo demineralization owing to lactate production by cells in the presence of insulin.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":" ","pages":"65-70"},"PeriodicalIF":1.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of substrate on the response of 3-dimensional periodontal ligament tissue to compressive stimulation. 基质对三维牙周韧带组织受压刺激反应的影响。
IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2025-04-16 Epub Date: 2025-03-08 DOI: 10.2334/josnusd.24-0366
Cheng Ni, Jun-Ichiro Jo, Ruonan Zhang, Yoshiya Hashimoto, Aki Nishiura

Purpose: The purpose of this study was to investigate the influence of substrates under the three-dimensional periodontal ligament (3D PDL) tissue on its biological functions after compressive stimulation.

Methods: A 3D PDL tissue was created using a poly(L-lactic acid) (PLLA) porous scaffold impregnated with human periodontal ligament fibroblasts (hPDLFs). It was then placed on a polyetheretherketone (PEEK) substrate, which has a comparable elastic modulus to bone and was compressed (25 g/cm2) for 1, 3, and 7 days. The morphology and biological functions of the hPDLFs in the 3D PDL tissue on the PEEK substrate were evaluated and compared with those on the polystyrene (PS) substrate.

Results: Compressive forces for the PLLA porous scaffold on the PEEK substrate were higher than those on the PS substrate. hPDLFs were present throughout the PLLA porous scaffold and there was no morphological change upon compressive stimulation. Increased expression of osteoclastogenic genes was observed after 3 days of compressive stimulation, while the level of these genes was increased by changing the substrate under the PDL tissue from PS to PEEK.

Conclusion: The substrate placed under the PDL tissue during compressive stimulation affects the biological functions of hPDLFs.

目的:研究三维牙周韧带(3D PDL)组织受压刺激后基质对其生物学功能的影响。方法:采用人牙周韧带成纤维细胞(hPDLFs)浸渍的聚l -乳酸(PLLA)多孔支架制备三维牙周韧带组织。然后将其放置在聚醚醚酮(PEEK)衬底上,该衬底具有与骨相当的弹性模量,并被压缩(25 g/cm2) 1,3和7天。研究了聚醚醚酮(PEEK)和聚苯乙烯(PS)基板上三维PDL组织中hPDLFs的形态和生物学功能。结果:聚乳酸多孔支架在PEEK基板上的压缩力高于PS基板上的压缩力。hPDLFs存在于PLLA多孔支架中,在压缩刺激下没有形态学变化。压缩刺激3天后,破骨基因的表达增加,而将PDL组织下的底物从PS改为PEEK后,这些基因的表达水平增加。结论:压缩刺激下置于PDL组织下的底物会影响hPDLFs的生物学功能。
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引用次数: 0
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Journal of oral science
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