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Journal of Plant Biology最新文献

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BEAR1, a bHLH Transcription Factor, Controls Salt Response Genes to Regulate Rice Salt Response bHLH转录因子BEAR1调控水稻盐反应基因
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2022-02-10 DOI: 10.1007/s12374-022-09347-4
Yantong Teng, Mingjie Lv, Xiangxiang Zhang, Maohong Cai, Tao Chen
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引用次数: 12
Hierarchical Structures and Dissected Functions of MADS-Box Transcription Factors in Rice Development MADS-Box转录因子在水稻发育中的层次结构和解剖功能
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2022-01-17 DOI: 10.1007/s12374-021-09343-0
Jinmi Yoon, Lae-Hyeon Cho, K. Jung
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引用次数: 3
Effect of Hydroxycinnamic Acid Amides, Coumaroyl Tyramine and Coumaroyl Tryptamine on Biotic Stress Response in Arabidopsis 羟基肉桂酸酰胺、香豆醇酪胺和香豆醇色胺对拟南芥生物胁迫响应的影响
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2022-01-06 DOI: 10.1007/s12374-021-09341-2
D. M. Macoy, S. Uddin, Gyeongik Ahn, Son Peseth, Gyeongryul Ryu, J. Cha, Jong-yeol Lee, Dongryeoul Bae, Seung-Mann Paek, Hye J. Chung, David Mackey, Sang Yeol Lee, Woe-Yeon Kim, M. G. Kim
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引用次数: 4
The Impact of Fasciation on Maize Inflorescence Architecture 迷恋对玉米花序结构的影响
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2022-01-04 DOI: 10.1007/s12374-021-09342-1
Da eun Kim, Jin-hee Jeong, Yu Mi Kang, Y. Park, Yongjae Lee, Jum-soon Kang, Y. Choi, Beung-Gu Son, S. Kim, Dave Jackson, B. Je
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引用次数: 4
Production of a Bacteria-like Particle Vaccine Targeting Rock Bream (Oplegnathus fasciatus) Iridovirus Using Nicotiana benthamiana. 利用本菌烟制备针对石鲷虹膜病毒的细菌样颗粒疫苗。
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2022-01-01 Epub Date: 2021-09-28 DOI: 10.1007/s12374-021-09328-z
Gyeongik Ahn, Joon-Yung Cha, Jeong Won Lee, Gyeongran Park, Gyeong-Im Shin, Shi-Jian Song, Gyeongryul Ryu, Inhwan Hwang, Min Gab Kim, Woe-Yeon Kim

Viral diseases are extremely widespread infections that change constantly through mutations. To produce vaccines against viral diseases, transient expression systems are employed, and Nicotiana benthamiana (tobacco) plants are a rapidly expanding platform. In this study, we developed a recombinant protein vaccine targeting the major capsid protein (MCP) of iridovirus fused with the lysine motif (LysM) and coiled-coil domain of coronin 1 (ccCor1) for surface display using Lactococcus lactis. The protein was abundantly produced in N. benthamiana in its N-glycosylated form. Total soluble proteins isolated from infiltrated N. benthamiana leaves were treated sequentially with increasing ammonium sulfate solution, and recombinant MCP mainly precipitated at 40-60%. Additionally, affinity chromatography using Ni-NTA resin was applied for further purification. Native structure analysis using size exclusion chromatography showed that recombinant MCP existed in a large oligomeric form. A minimum OD600 value of 0.4 trichloroacetic acid (TCA)-treated L. lactis was required for efficient recombinant MCP display. Immunogenicity of recombinant MCP was assessed in a mouse model through enzyme-linked immunosorbent assay (ELISA) with serum-injected recombinant MCP-displaying L. lactis. In summary, we developed a plant-based recombinant vaccine production system combined with surface display on L. lactis.

病毒性疾病是一种极其广泛的传染病,通过突变不断发生变化。为了生产针对病毒性疾病的疫苗,瞬态表达系统被采用,而烟(烟草)植物是一个迅速扩展的平台。本研究以虹膜病毒的主要衣壳蛋白(MCP)为靶点,与赖氨酸基序(LysM)和冠状蛋白1的卷曲结构域(ccCor1)融合,利用乳酸乳球菌进行表面展示。该蛋白在n -糖基化形式的benthamiana中大量产生。通过增加硫酸铵溶液的浓度,对侵染的红叶总可溶性蛋白进行顺序处理,重组MCP主要沉淀在40 ~ 60%。采用Ni-NTA树脂亲和层析进一步纯化。用粒径不相容色谱法进行天然结构分析表明,重组MCP以大的低聚形式存在。在三氯乙酸(TCA)处理的乳酸菌中,OD600值至少为0.4才能有效地显示重组MCP。采用酶联免疫吸附法(ELISA)评价重组MCP在小鼠模型中的免疫原性。综上所述,我们开发了一种结合乳杆菌表面展示的植物重组疫苗生产体系。
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引用次数: 1
Transcriptome Analysis Reveals Molecular Mechanisms Underlying Methyl Jasmonate-mediated Biosynthesis of Protopanaxadiol-type Saponins in Panax notoginseng Leaves 转录组分析揭示三七叶片中茉莉酸甲酯介导的原人参二醇型皂苷生物合成的分子机制
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2021-11-03 DOI: 10.1007/s12374-021-09331-4
Ying Li, Yuan Lin, B. Jia, Geng Chen, H. Shi, Rui Xu, Xuejiao Li, Junrong Tang, Qingyan Tang, Guanghui Zhang, Jian-li Yang, W. Fan, Sheng-chao Yang
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引用次数: 4
Overexpression of DREB2C Delays Flowering in Arabidopsis thaliana via the Activation of FLC DREB2C过表达通过激活FLC延迟拟南芥开花
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2021-10-18 DOI: 10.1007/s12374-021-09337-y
Chieun Song, Juwan Baek, Usol Choe, C. Lim
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引用次数: 2
Heterogeneous Overexpression of Two Oryza sativa Arsenic-Induced RING E3 Ligase4 (OsAIR4.1 and 4.2) Transcripts Enhances Plant Tolerance to Arsenic Stress 砷诱导的两个水稻RING E3连接酶4(OsAIR4.1和4.2)转录物的异质性过表达增强了植物对砷胁迫的耐受性
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2021-10-15 DOI: 10.1007/s12374-021-09336-z
J. Kim, Hyeon Ung Seo, J. E. Lee, S. Lim, C. Jang
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引用次数: 2
A Method for a Rapid and Accurate Detection of Phloem Sieve Element Development: Its Application and Scalability 一种快速、准确的韧皮部筛元检测方法的研制及其应用与可扩展性
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2021-10-07 DOI: 10.1007/s12374-021-09334-1
Hyoujin Kim, Ji-Young Lee
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引用次数: 0
Overexpression of BdASR2 Plays a Positive Role in Response to Salt Tolerance in Brachypodium distachyon BdASR2的过表达在短柄草的耐盐性响应中起积极作用
IF 2.9 4区 生物学 Q2 PLANT SCIENCES Pub Date : 2021-09-30 DOI: 10.1007/s12374-021-09333-2
Jin Seok Yoon, Paulina Calderon Flores, Y. Seo
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引用次数: 3
期刊
Journal of Plant Biology
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