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Evaluation and Stability Analysis of Early Maturing Soybean (Glycine Max L.) Genotypes in Lowland Agro Ecologies of Ethiopia 早熟大豆(Glycine Max L.)的评价与稳定性分析埃塞俄比亚低地农业生态的基因型
Pub Date : 2023-07-31 DOI: 10.11648/j.jps.20231104.14
Masreshaw Yirga, Yechalew Sileshi, Demelash Bassa
: Soybean is becoming the most important oil crops in Ethiopia. The national soybean breeding program is working to develop high-yielding, adaptable and stable varieties to increase production and productivity in the country. Breeding for early-maturing varieites is currently given due emphasis to overcome soybean production drawbacks in moisture defecit areas. Hence, performance evaluation of early maturing soybean genotypes across representative environments is essential to examine genotype × environment (GxE) interactions and identify the most stable and performed genotype. The trial was conducted using 10 soybean genotypes including checks in RCB design with four replications. The materials were evaluated over eight locations and G x E interaction was assessed using GGE-biplot analysis to identify stable genotype across testing environments. Data on phenological, agro-morphological and reaction to important diseases were collected for the crop. The combined analysis of variances showed significant to highly significant (P ˂ 0.01) difference among genotypes, environments and G x E interactions for most of the studied traits. GGE-biplot models showed that the eight environments used for the study belonged to four mega-environments. According to the GGE results, G3 (JM-HAR/G99-15-SD-2), and G7 (JM-HAR/PR142-15-SB) were identified as ideal genotypes in terms of higher-yielding ability and stability, and hence thesegenotypes are recommended for mega environment production in the country.
大豆正在成为埃塞俄比亚最重要的油料作物。国家大豆育种计划正在努力开发高产、适应性强和稳定的品种,以提高该国的产量和生产力。目前,早熟品种的选育受到了应有的重视,以克服水分不足地区大豆生产的缺点。因此,早熟大豆基因型在代表性环境下的性能评价对于检验基因型与环境(GxE)之间的相互作用并确定最稳定和表现最好的基因型至关重要。试验采用10个大豆基因型,包括RCB设计检查,共4个重复。在8个地点对材料进行评估,并使用ge -双图分析评估gx E相互作用,以确定不同测试环境下的稳定基因型。收集了该作物物候、农业形态和对重要病害的反应等资料。综合方差分析表明,基因型、环境和gx E交互作用对大多数性状的影响显著至极显著(P值小于0.01)。ge -双标图模型显示,研究中使用的8个环境属于4个巨型环境。根据GGE结果,G3 (JM-HAR/G99-15-SD-2)和G7 (JM-HAR/PR142-15-SB)在高产能力和稳定性方面被确定为理想的基因型,因此这些基因型推荐用于国内大型环境生产。
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引用次数: 0
Soybean (Glycine max) Yield Loss Due to Weeds at Jimma, Ethiopia 埃塞俄比亚Jimma杂草造成的大豆(甘氨酸max)产量损失
Pub Date : 2023-07-26 DOI: 10.11648/j.jps.20231104.13
Tigist Bidira, T. Shimales, Tekleeyesus Firde
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引用次数: 0
Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR 利用Real-Time PCR技术快速鉴别高价值阔叶木——大胡桃木
Pub Date : 2023-07-17 DOI: 10.11648/j.jps.20231104.12
Jiaying Wang, Jihong Zhang, Weijun Duan, Xianfeng Chen, Yan Wang
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引用次数: 0
Stimulatory Effects of Flakes and Compost Amendment Based on Tithonia diversifolia on the Quality of PIF Plantain Seedlings Growth and Tolerance to Mycosphaerella fijiensis 大车前草叶片和堆肥添加剂对PIF大车前草幼苗生长质量和对斐济分枝杆菌抗性的促进作用
Pub Date : 2023-07-17 DOI: 10.11648/j.jps.20231104.11
Meshuneké Arouna, Dakolé Daboy Charles, Wassom Damien Fabrice, Fotsing Silatsa Landry, Elock Mbang Gaston, Kengoum Djam Marie Paule, Ché William Asah, Kom Timma Jospin Walter, Niemenak Nicolas, Boudjeko Thaddée, Ewané Cécile Annie
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引用次数: 0
Participatory Demonstration and Evaluation of Common Bean Technologies in Lowlands of Borana, Oromia National Regional State, Ethiopia 埃塞俄比亚奥罗米亚民族地区州博拉纳低地普通豆技术参与式示范与评价
Pub Date : 2023-06-27 DOI: 10.11648/j.jps.20231103.18
Feyissa Desiso
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引用次数: 0
The Characterization of Anatomical Indices of Seed Coat Formation of Diploid and Tetraploid Representatives of the Genus Gossypium L 棉属二倍体和四倍体代表种皮形成解剖指标的研究
Pub Date : 2023-06-27 DOI: 10.11648/j.jps.20231103.17
Nina Tutushkina, Abdumavlyan Abdullaev, Ziroat Ernazarova
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引用次数: 0
Distribution and Regeneration Status of Guibourtia copallifera Benn. in Sierra Leone 黄花桂的分布与更新状况。在塞拉利昂
Pub Date : 2023-05-29 DOI: 10.11648/j.jps.20231103.11
J. Johnny, Aiah Lebbie, R. Wadsworth
: This study was conducted to assess the species distributional range, structure, and regeneration status of G. copallifera Benn. A distributional survey was conducted in over 30 villages and communities where claims were made about the presence of this species. At four locations, pairs of large plots measuring 500m x 20m were laid out 400m apart and each subdivided into ten (10) subplots (50m x 20m). For the regeneration studies (Seeds, seedlings and saplings), four quadrats of 5m x 5m were established in each subplot (giving a total of 320 quadrats). A total of 1,294 live individuals from 74 tree species and 12 lianas were recorded, representing 61 genera in 27 families. Guibourtia copallifera has the highest dominance, followed by Nesogordonia papaveriefera (A. Chev.) Capuron ex N. Hallé. , Memecylon normandii Jacq.-Fél. and Gmelina arborea Roxb . Species such as Homalium africanum (Hook. f.) Benth. Lindackeria dentata (Oliv.) Gilg , Pentadesma butyracea Sabine. and Santiria trimera (Oliv.) Aubrév. were recorded at low levels. Regarding family dominance, the Leguminosae - Caesalpiniaceae scores the highest, followed by Sterculiaceae, Euphorbiaceae, Melastomataceae and Verbenaceae. The density of regenerating individuals varied significantly between subplots based on the amount of degradation. The regeneration and spatial patterns of the species examined in this study exhibited clear relationships with disturbance intensity. Urgent action is needed to conserve this species, together with other ecologically and economically important tree species, before they are locally exterminated.
摘要:本文研究了黄杨属植物的分布范围、结构和更新状况。在声称存在该物种的30多个村庄和社区进行了分布调查。在四个地点,面积为500米× 20米的大地块相隔400米,每个地块被细分为10个子地块(50米× 20米)。对于再生研究(种子、幼苗和树苗),在每个子样地建立4个5m × 5m的样方(总共320个样方)。共记录到74种乔木、12种藤本植物、27科61属1294株活株。Guibourtia copallifera的优势度最高,其次是Nesogordonia papaveriefera (A. Chev.)。卡普隆前n·哈勒维尔。, Memecylon normandii jacq .- fsami。Gmelina arborea Roxb。如非洲Homalium africanum(钩。f。)Benth。齿形林氏菌(橄榄)丁酸戊二酯。和桑提亚·特里梅拉(奥利弗)Aubrev。被记录在低水平。在科优势度方面,豆科(Leguminosae - Caesalpiniaceae)得分最高,其次是Sterculiaceae、Euphorbiaceae、Melastomataceae和马鞭草科(Verbenaceae)。不同亚样地再生个体密度随退化程度的不同而有显著差异。在本研究中,物种的更新和空间格局与干扰强度有明显的关系。我们需要采取紧急行动,保护这一物种,以及其他具有重要生态和经济意义的树种,以免它们在当地被灭绝。
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引用次数: 0
Performance Evaluation of Potato Genotypes for Yield, Yield Related Traits in Bale High Lands South Eastern Ethiopia 埃塞俄比亚东南部贝尔高地马铃薯基因型产量及相关性状的性能评价
Pub Date : 2023-05-18 DOI: 10.11648/j.jps.20231102.12
G. Asefa, M. Beriso, Gemmechu Ijigu
: Potato is an important food security crop in the world spacially for Africa, specifically Ethiopia which ranked 9 th in potato production in Africa. Bale Zone South Eastern Ethiopia is known for potato production both in irrigation and rainfed. Howevere, the productivity of the crop is low due to many factors among which lack of improved potato varieties which were high yeilder and tolerance to potato disease is major. This study was conducted to evaluate potato genotypes for high-yielding, stability and resistance to late blight. The experiment was laid out in a randomized complete block design with three replications at three locations (Sinana, Goba and Dinsho districts) for three years. The highest total tuber yield was recorded from genotypes CIP-392640-524 (45.75t/ha -1 ) followed by CIP-395114.5 (40.01t/ha -1 ) while the lowest total tuber yield was obtained from local checks (25.27t/ha -1 ). Result from stability papameters indicated the stability of tested genotypes over the tested environment. The yield performance of genotypes (CIP-392640-524) across environments and yield advantage of genotype over standard checks (Moti) 11.45% with less disease (late blight reaction) and the result from AMMI analysis of variance and stability parameter also indicated the stability of genotype over tested environments. This suggested that the genotypes are promoted to a variety of verification trials for Bale highlands and similar agro-ecologies.
马铃薯是世界上重要的粮食安全作物,特别是非洲,特别是埃塞俄比亚,其马铃薯产量在非洲排名第9位。埃塞俄比亚东南部的贝尔区以灌溉和雨养马铃薯生产而闻名。然而,由于多种因素的影响,作物的产量较低,其中主要是缺乏高产和抗病的马铃薯改良品种。本研究对马铃薯高产、稳定和抗晚疫病的基因型进行了评价。实验采用随机完全区设计,在三个地点(Sinana、Goba和Dinsho区)进行三次重复,为期三年。CIP-392640-524基因型块茎总产量最高(45.75t/ha -1),其次是CIP-395114.5基因型(40.01t/ha -1),而本地检测的块茎总产量最低(25.27t/ha -1)。稳定性参数的结果表明所测基因型在测试环境中的稳定性。基因型(CIP-392640-524)在不同环境下的产量表现和相对于标准对照(Moti)的产量优势11.45%,且发病较少(晚疫病反应),AMMI方差分析和稳定性参数分析结果也表明基因型在不同环境下的稳定性。这表明这些基因型可以推广到贝尔高原和类似农业生态的各种验证试验中。
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引用次数: 0
DNA Extraction from Heartwood and Quick Species Authentication Using Real-Time PCR: A Case Study of the Rosewood (Pterocarpus Indicus) 心材DNA提取及实时荧光定量PCR快速鉴定——以红木(Pterocarpus Indicus)为例
Pub Date : 2023-04-27 DOI: 10.11648/j.jps.20231102.11
Jihong Zhang, Jiaying Wang, Xu Ying, Xiaoling Lv, Jianhua Wei, Ma Ming, Junxia Cui
: Illegal logging, felling and timber trade have continued to increase over the past two decades, leading to a decline in forest biodiversity and the extinction of some wood species. Pterocarpus indicus Willd., listed in the National Standards of the People’s Republic of China for Hongmu (GB/T 18107-2017), is widely used in production of high-end furniture, decorative flooring and musical instruments due to its high-quality timber. For molecular species identification, the quality and quantity of DNA extracted from wood samples should first be ensured. However, extracting DNA from dried, aged timber heartwood is difficult, as heartwood contains little fragmented DNA, along with lots of phenolic compounds known to impede sequence amplification. In order to protect P. indicus from over-exploitation and to achieve accurate species-level identification, we established a particular extraction method for obtaining amplifiable DNA from heartwood samples and the real-time PCR assay for species discrimination of P. indicus in this study. The quantity and quality of DNA extracted from dry heartwood samples using the modified CTAB method were 2.40-37.70 ng/µL and 1.55-2.12 demonstrated by OD 260/280 , respectively. Primer set P9, targeting P. indicus specific microsatellite Pin2-20 sequence, was amplifiable in newly established real-time PCR. Through analysis, this real time PCR was shown to be specific and sensitive with a detection limit around 0.17 ng/µL. Hopefully, this study will contribute to heartwood DNA extraction and species identification of timber logs for forensic discrimination, law enforcement and natural resource conservation.
过去二十年来,非法采伐、砍伐和木材贸易持续增加,导致森林生物多样性下降,一些木材物种灭绝。野龙柏。被列入中华人民共和国国家标准《红木》(GB/T 18107-2017),因其优质的木材被广泛用于生产高档家具、装饰地板和乐器。对于分子物种鉴定,首先要保证木材样本DNA提取的质量和数量。然而,从干燥、老化的木材心材中提取DNA是困难的,因为心材含有很少的DNA碎片,以及许多已知会阻碍序列扩增的酚类化合物。为了防止籼稻被过度开发,实现准确的种级鉴定,本研究建立了一种从心材样品中提取可扩增DNA的特殊提取方法,并建立了籼稻种级鉴别的实时PCR检测方法。改进的CTAB法提取干心材样品DNA的数量和质量分别为2.40 ~ 37.70 ng/µL和1.55 ~ 2.12 ng/µL (OD值为260/280)。引物组P9在新建立的实时PCR中可扩增,该引物组针对籼稻特有的微卫星Pin2-20序列。分析结果表明,该实时PCR具有特异性和敏感性,检测限约为0.17 ng/µL。希望本研究能够为心材DNA提取和物种鉴定提供依据,为法医鉴定、执法和自然资源保护提供依据。
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引用次数: 0
Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus 烟草摇铃病毒快速筛选微流控芯片方法的建立
Pub Date : 2023-03-09 DOI: 10.11648/j.jps.20231101.14
Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun
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引用次数: 0
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Journal of Plant Sciences
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