首页 > 最新文献

Journal of Reproduction and Development最新文献

英文 中文
Factors influencing in vivo embryo production in Japanese Black donors: the role of Anti-Müllerian hormone and inflammation parameters.
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-31 DOI: 10.1262/jrd.2024-092
Hiroaki Okawa, Norihiro Yukiyama, Osamu Yamato, Akira Goto, Oky Setyo Widodo, Yasuo Fushimi, Mitsuhiro Takagi

In Japanese Black (JB) cattle, the number of transferable embryos produced after superovulation is crucial for the economic success of embryo production for both farmers and veterinarians. Anti-Müllerian hormone (AMH) has emerged as a key reproductive marker for predicting the number of embryos produced in vivo and oocytes retrieved through transvaginal pickup. This study investigated the relationship between AMH, inflammatory markers, including serum amyloid A (SAA) and albumin/globulin (A/G) ratio, and the number of embryos recovered and transferable after superovulation in JB cows. A total of 96 JB donor cows underwent artificial insemination after superovulation, and embryo retrieval was performed 7 days later. Embryos retrieved were classified based on the International Embryo Technology Society criteria, wherein "transferable embryos" included those with codes 1 or 2, while "total embryos" included transferable embryos as well as those with codes 3 and 4. Blood samples collected during embryo recovery were used to measure serum AMH, SAA, and A/G ratios. When grouped by AMH quartiles, the high-AMH and middle-high-AMH groups produced significantly more total embryos compared to the low-AMH group. The total number of embryos increased with higher AMH levels (r = 0.3336, P = 0.0009). Correlation analysis revealed associations between AMH, α1-globulin and SAA. Additionally, a significant positive correlation was observed between total and transferable embryos (r = 0.6339, P < 0.0001) and between AMH and the yield ratio (r = 0.25583, P = 0.0119). These findings confirm that AMH concentration is a valuable reproductive marker for predicting the total and transferable embryos produced by JB donor cows.

{"title":"Factors influencing in vivo embryo production in Japanese Black donors: the role of Anti-Müllerian hormone and inflammation parameters.","authors":"Hiroaki Okawa, Norihiro Yukiyama, Osamu Yamato, Akira Goto, Oky Setyo Widodo, Yasuo Fushimi, Mitsuhiro Takagi","doi":"10.1262/jrd.2024-092","DOIUrl":"https://doi.org/10.1262/jrd.2024-092","url":null,"abstract":"<p><p>In Japanese Black (JB) cattle, the number of transferable embryos produced after superovulation is crucial for the economic success of embryo production for both farmers and veterinarians. Anti-Müllerian hormone (AMH) has emerged as a key reproductive marker for predicting the number of embryos produced in vivo and oocytes retrieved through transvaginal pickup. This study investigated the relationship between AMH, inflammatory markers, including serum amyloid A (SAA) and albumin/globulin (A/G) ratio, and the number of embryos recovered and transferable after superovulation in JB cows. A total of 96 JB donor cows underwent artificial insemination after superovulation, and embryo retrieval was performed 7 days later. Embryos retrieved were classified based on the International Embryo Technology Society criteria, wherein \"transferable embryos\" included those with codes 1 or 2, while \"total embryos\" included transferable embryos as well as those with codes 3 and 4. Blood samples collected during embryo recovery were used to measure serum AMH, SAA, and A/G ratios. When grouped by AMH quartiles, the high-AMH and middle-high-AMH groups produced significantly more total embryos compared to the low-AMH group. The total number of embryos increased with higher AMH levels (r = 0.3336, P = 0.0009). Correlation analysis revealed associations between AMH, α<sub>1</sub>-globulin and SAA. Additionally, a significant positive correlation was observed between total and transferable embryos (r = 0.6339, P < 0.0001) and between AMH and the yield ratio (r = 0.25583, P = 0.0119). These findings confirm that AMH concentration is a valuable reproductive marker for predicting the total and transferable embryos produced by JB donor cows.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of nuclear receptor corepressor 2 (NCOR2) in estrogen-induced repression of arcuate Kiss1 expression in female rats.
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-27 DOI: 10.1262/jrd.2024-100
Marina Takizawa, Sae Miyazaki, Hitomi Tsuchida, Mayuko Nagae, Shunsuke Seki, Masumi Hirabayashi, Fumitaka Osakada, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama

Hypothalamic arcuate (ARC) kisspeptin neurons are considered the gonadotropin-releasing hormone pulse generator in rats. In virgin rats, the expression of the ARC kisspeptin gene (Kiss1) is repressed by proestrous levels of estradiol-17β (high E2) but not by diestrous levels of E2 (low E2). In lactating rats, ARC Kiss1 expression is repressed by low E2 during late lactation. This study aimed to investigate whether nuclear receptor corepressor 2 (NCOR2, encoded by Ncor2), an estrogen receptor α corepressor, is involved in the estrogen-induced repression of ARC Kiss1 expression in rats. Double in situ hybridization for Kiss1 and Ncor2 revealed that approximately 80% of ARC Kiss1-expressing cells co-expressed Ncor2 in ovariectomized (OVX) + low E2 virgin rats, while approximately 90% of ARC Kiss1-expressing cells co-expressed Ncor2 in OVX + low E2 lactating rats. To further examine the role of Ncor2, we studied the effects of Kiss1-dependent Ncor2 knockdown on ARC Kiss1 expression and luteinizing hormone (LH) pulses. An adeno-associated virus vector carrying Cre-activated short hairpin RNA (shRNA) for Ncor2 was administered to the ARC in two Kiss1-Cre rat models: OVX + high E2 Kiss1-Cre virgin rats and OVX + low E2 Kiss1-Cre lactating rats. Ncor2-shRNA treatment significantly increased the number of ARC Kiss1-expressing cells and the intensity of Kiss1 signals in OVX + high E2 virgin rats but failed to fully restore low E2-induced Kiss1 repression in lactating rats. The Ncor2-shRNA treatment failed to affect LH pulses in both models. These findings suggest that NCOR2 in ARC kisspeptin neurons mediates high E2-induced repression of ARC Kiss1 expression in virgin rats.

{"title":"Involvement of nuclear receptor corepressor 2 (NCOR2) in estrogen-induced repression of arcuate Kiss1 expression in female rats.","authors":"Marina Takizawa, Sae Miyazaki, Hitomi Tsuchida, Mayuko Nagae, Shunsuke Seki, Masumi Hirabayashi, Fumitaka Osakada, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama","doi":"10.1262/jrd.2024-100","DOIUrl":"https://doi.org/10.1262/jrd.2024-100","url":null,"abstract":"<p><p>Hypothalamic arcuate (ARC) kisspeptin neurons are considered the gonadotropin-releasing hormone pulse generator in rats. In virgin rats, the expression of the ARC kisspeptin gene (Kiss1) is repressed by proestrous levels of estradiol-17β (high E2) but not by diestrous levels of E2 (low E2). In lactating rats, ARC Kiss1 expression is repressed by low E2 during late lactation. This study aimed to investigate whether nuclear receptor corepressor 2 (NCOR2, encoded by Ncor2), an estrogen receptor α corepressor, is involved in the estrogen-induced repression of ARC Kiss1 expression in rats. Double in situ hybridization for Kiss1 and Ncor2 revealed that approximately 80% of ARC Kiss1-expressing cells co-expressed Ncor2 in ovariectomized (OVX) + low E2 virgin rats, while approximately 90% of ARC Kiss1-expressing cells co-expressed Ncor2 in OVX + low E2 lactating rats. To further examine the role of Ncor2, we studied the effects of Kiss1-dependent Ncor2 knockdown on ARC Kiss1 expression and luteinizing hormone (LH) pulses. An adeno-associated virus vector carrying Cre-activated short hairpin RNA (shRNA) for Ncor2 was administered to the ARC in two Kiss1-Cre rat models: OVX + high E2 Kiss1-Cre virgin rats and OVX + low E2 Kiss1-Cre lactating rats. Ncor2-shRNA treatment significantly increased the number of ARC Kiss1-expressing cells and the intensity of Kiss1 signals in OVX + high E2 virgin rats but failed to fully restore low E2-induced Kiss1 repression in lactating rats. The Ncor2-shRNA treatment failed to affect LH pulses in both models. These findings suggest that NCOR2 in ARC kisspeptin neurons mediates high E2-induced repression of ARC Kiss1 expression in virgin rats.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats.
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-25 DOI: 10.1262/jrd.2024-078
Yuki Koyano, Masakatsu Fujinoki

The neurotransmitter, 5-hydroxytriptamine (5-HT), is well known. Furthermore, it enhances the acrosome reaction, hyperactivation, and in vitro fertilization (IVF) success in hamsters and mice. In the present study, we examined whether 5-HT enhances hyperactivation and increases IVF success in rats. When rat sperm was exposed to 5-HT, hyperactivation was significantly enhanced. Only the 5-HT4 receptor agonists significantly enhanced hyperactivation. Additionally, both 5-HT and 5-HT4 receptor agonists significantly increase the success of IVF. These results suggested that 5-HT increases IVF success by enhancing hyperactivation and effects of 5-HT are associated with the 5-HT4 receptor. Therefore, in rats, 5-HT enhances capacitation and the 5-HT4 receptor is the key molecule for capacitation.

{"title":"Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats.","authors":"Yuki Koyano, Masakatsu Fujinoki","doi":"10.1262/jrd.2024-078","DOIUrl":"https://doi.org/10.1262/jrd.2024-078","url":null,"abstract":"<p><p>The neurotransmitter, 5-hydroxytriptamine (5-HT), is well known. Furthermore, it enhances the acrosome reaction, hyperactivation, and in vitro fertilization (IVF) success in hamsters and mice. In the present study, we examined whether 5-HT enhances hyperactivation and increases IVF success in rats. When rat sperm was exposed to 5-HT, hyperactivation was significantly enhanced. Only the 5-HT<sub>4</sub> receptor agonists significantly enhanced hyperactivation. Additionally, both 5-HT and 5-HT<sub>4</sub> receptor agonists significantly increase the success of IVF. These results suggested that 5-HT increases IVF success by enhancing hyperactivation and effects of 5-HT are associated with the 5-HT<sub>4</sub> receptor. Therefore, in rats, 5-HT enhances capacitation and the 5-HT<sub>4</sub> receptor is the key molecule for capacitation.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Can thermoregulatory response to heat stress be improved in lactating dairy cows? Insights from counter-current heat transfer systems impacting reproduction. 泌乳奶牛对热应激的体温调节反应能否得到改善?从影响生殖的逆流传热系统的见解。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-16 DOI: 10.1262/jrd.2024-101
Fernando López-Gatius

Climate change has caused heat stress (HS) to become an increasingly severe problem for high-producing dairy herds. Although cooling systems allow milk production to remain nearly constant throughout the year, fertility decreases during summer. Physiological counter-current heat transfer mechanisms maintaining brain/hypothalamic and reproductive functions in cattle are vulnerable to HS. In this study, I propose strategies to improve cooling systems, particularly in zones with the highest risk of increased body temperature, such as milking areas. In addition, heat transfer mechanisms to protect the brain-hypothalamus axis from hyperthermia must be considered when implementing measures to reduce HS-related problems.

气候变化导致热应激(HS)成为高产奶牛群日益严重的问题。尽管冷却系统使牛奶产量在一年中几乎保持不变,但在夏季,生育率会下降。维持牛脑/下丘脑和生殖功能的生理逆流传热机制易受HS影响。在这项研究中,我提出了改善冷却系统的策略,特别是在体温升高风险最高的地区,如挤奶区。此外,在实施减少高温相关问题的措施时,必须考虑保护脑-下丘脑轴免受高温影响的传热机制。
{"title":"Can thermoregulatory response to heat stress be improved in lactating dairy cows? Insights from counter-current heat transfer systems impacting reproduction.","authors":"Fernando López-Gatius","doi":"10.1262/jrd.2024-101","DOIUrl":"https://doi.org/10.1262/jrd.2024-101","url":null,"abstract":"<p><p>Climate change has caused heat stress (HS) to become an increasingly severe problem for high-producing dairy herds. Although cooling systems allow milk production to remain nearly constant throughout the year, fertility decreases during summer. Physiological counter-current heat transfer mechanisms maintaining brain/hypothalamic and reproductive functions in cattle are vulnerable to HS. In this study, I propose strategies to improve cooling systems, particularly in zones with the highest risk of increased body temperature, such as milking areas. In addition, heat transfer mechanisms to protect the brain-hypothalamus axis from hyperthermia must be considered when implementing measures to reduce HS-related problems.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system. 利用 CRISPR/Cas9 系统,通过脂质转染两种引导 RNA 对猪子一代进行基因组编辑。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-08-30 DOI: 10.1262/jrd.2024-054
Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara, Takeshige Otoi

CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GHR) and glycoprotein alpha-galactosyltransferase 1 (GGTA1)-targeting guide RNAs (gRNAs) in pig zygotes. Zona pellucida-free zygotes were collected 10 h after in vitro fertilization and incubated with Cas9, gRNAs, and Lipofectamine 2000 (LP2000) for 5 h. In Experiment 1, we evaluated the mutation efficiency of gRNAs targeting either GHR or GGTA1 in zygotes transfected using LP2000 and cultured in 4-well plates. In Experiment 2, we examined the effects of the culture method on the development, mutation rate, and mutation efficiency of zygotes with simultaneously double-edited GHR and GGTA1, cultured using 4-well (group culture) and 25-well plates (individual culture). In Experiment 3, we assessed the effect of additional GHR-targeted lipofection before and after simultaneous double gRNA-targeted lipofection on the mutation efficiency of edited embryos cultured in 25-well plates. No significant differences in mutation rates were observed between the zygotes edited with either gRNA. Moreover, the formation rate of blastocysts derived from GHR and GGTA1 double-edited zygotes was significantly increased in the 25-well plate culture compared to that in the 4-well plate culture. However, mutations were only observed in GGTA1 when zygotes were transfected with both gRNAs, irrespective of the culture method used. GHR mutations were detected only in blastocysts derived from zygotes subjected to GHR-targeted lipofection before simultaneous double gRNA-targeted lipofection. Overall, our results suggest that additional lipofection before simultaneous double gRNA-targeted lipofection induces additional mutations in the zygotes.

通过电穿孔进行基于CRISPR/Cas9的多重基因组编辑相对高效;然而,脂质体感染因其易用性和低成本而用途广泛。在这里,我们的目的是利用生长激素受体(GHR)和糖蛋白α-半乳糖基转移酶1(GGTA1)靶向向导RNA(gRNAs)确定脂质体感染在基于CRISPR/Cas9的多重基因组编辑中的效率。在实验 1 中,我们评估了使用 LP2000 转染并在 4 孔板中培养的无透明带子代中靶向 GHR 或 GGTA1 的 gRNA 的突变效率。在实验 2 中,我们检测了培养方法对同时进行 GHR 和 GGTA1 双编辑的子代的发育、突变率和突变效率的影响,这些子代分别用 4 孔板(群体培养)和 25 孔板(个体培养)培养。在实验 3 中,我们评估了在同时进行双 gRNA 靶向脂质转染之前和之后进行额外的 GHR 靶向脂质转染对在 25 孔板中培养的编辑胚胎突变效率的影响。使用两种 gRNA 编辑的胚胎在突变率上没有明显差异。此外,与 4 孔板培养相比,25 孔板培养的 GHR 和 GGTA1 双编辑子代胚胎的囊胚形成率明显增加。然而,无论使用哪种培养方法,只有在转染两种 gRNA 的子囊胚胎中才能观察到 GGTA1 的突变。只有在同时进行双 gRNA 靶向脂质体转染之前进行 GHR 靶向脂质体转染的子囊所产生的囊胚中才检测到 GHR 突变。总之,我们的研究结果表明,在同时进行双 gRNA 靶向脂质感染之前进行额外的脂质感染会诱导子囊中出现额外的突变。
{"title":"Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.","authors":"Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara, Takeshige Otoi","doi":"10.1262/jrd.2024-054","DOIUrl":"10.1262/jrd.2024-054","url":null,"abstract":"<p><p>CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GHR) and glycoprotein alpha-galactosyltransferase 1 (GGTA1)-targeting guide RNAs (gRNAs) in pig zygotes. Zona pellucida-free zygotes were collected 10 h after in vitro fertilization and incubated with Cas9, gRNAs, and Lipofectamine 2000 (LP2000) for 5 h. In Experiment 1, we evaluated the mutation efficiency of gRNAs targeting either GHR or GGTA1 in zygotes transfected using LP2000 and cultured in 4-well plates. In Experiment 2, we examined the effects of the culture method on the development, mutation rate, and mutation efficiency of zygotes with simultaneously double-edited GHR and GGTA1, cultured using 4-well (group culture) and 25-well plates (individual culture). In Experiment 3, we assessed the effect of additional GHR-targeted lipofection before and after simultaneous double gRNA-targeted lipofection on the mutation efficiency of edited embryos cultured in 25-well plates. No significant differences in mutation rates were observed between the zygotes edited with either gRNA. Moreover, the formation rate of blastocysts derived from GHR and GGTA1 double-edited zygotes was significantly increased in the 25-well plate culture compared to that in the 4-well plate culture. However, mutations were only observed in GGTA1 when zygotes were transfected with both gRNAs, irrespective of the culture method used. GHR mutations were detected only in blastocysts derived from zygotes subjected to GHR-targeted lipofection before simultaneous double gRNA-targeted lipofection. Overall, our results suggest that additional lipofection before simultaneous double gRNA-targeted lipofection induces additional mutations in the zygotes.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"356-361"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MiR-145-5p regulates granulosa cell proliferation by targeting the SET gene in KGN cells. MiR-145-5p通过靶向KGN细胞中的SET基因调节颗粒细胞增殖。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-09-22 DOI: 10.1262/jrd.2024-053
Gao Lingling, Yang Qingxing, Xu Jianbo, Wang Weijie, Lu Dan

MiR-145-5p has been implicated in the development and progression of various disorders, and it is primarily recognized as a tumor suppressor in numerous cancers types. Its expression has been reported to decrease in the granulosa cells of patients with polycystic ovarian syndrome (PCOS). This study aimed to investigate whether miR-145-5p plays a role in granulosa cell proliferation and to shed light on the underlying pathological mechanisms of follicular development in patients with PCOS. Follicular fluid samples were collected from patients with PCOS and healthy individuals. The Cell Counting Kit-8 and bromodeoxyuridine assays were performed to assess KGN cell proliferation. The expression of miR-145-5p was significantly decreased in PCOS granulosa cells than in control cells, whereas the expression of SET was increased. Furthermore, miR-145-5p suppressed the proliferation of KGN cells. SET was identified as a direct target of miR-145-5p. Additionally, SET promoted the proliferation of KGN cells, and knockdown of SET counteracted the effect of the miR-145-5p inhibitor. Therefore, miR-145-5p regulates granulosa cell proliferation by targeting the SET in KGN cells; this process may be a potential pathological pathway that contributes to follicular developmental disorders in PCOS.

MiR-145-5p与多种疾病的发生和发展有关,它主要被认为是多种类型癌症的肿瘤抑制因子。据报道,在多囊卵巢综合征(PCOS)患者的颗粒细胞中,它的表达量有所下降。本研究旨在探讨 miR-145-5p 是否在颗粒细胞增殖中发挥作用,并揭示多囊卵巢综合征患者卵泡发育的潜在病理机制。研究人员采集了多囊卵巢综合征患者和健康人的卵泡液样本。采用细胞计数试剂盒-8和溴脱氧尿苷检测法评估KGN细胞的增殖情况。与对照细胞相比,多囊卵巢综合征颗粒细胞中 miR-145-5p 的表达明显减少,而 SET 的表达则有所增加。此外,miR-145-5p 还抑制了 KGN 细胞的增殖。SET被确定为miR-145-5p的直接靶标。此外,SET能促进KGN细胞的增殖,而敲除SET能抵消miR-145-5p抑制剂的作用。因此,miR-145-5p通过靶向KGN细胞中的SET来调节颗粒细胞的增殖;这一过程可能是导致多囊卵巢综合症患者卵泡发育障碍的潜在病理途径。
{"title":"MiR-145-5p regulates granulosa cell proliferation by targeting the <i>SET</i> gene in KGN cells.","authors":"Gao Lingling, Yang Qingxing, Xu Jianbo, Wang Weijie, Lu Dan","doi":"10.1262/jrd.2024-053","DOIUrl":"10.1262/jrd.2024-053","url":null,"abstract":"<p><p>MiR-145-5p has been implicated in the development and progression of various disorders, and it is primarily recognized as a tumor suppressor in numerous cancers types. Its expression has been reported to decrease in the granulosa cells of patients with polycystic ovarian syndrome (PCOS). This study aimed to investigate whether miR-145-5p plays a role in granulosa cell proliferation and to shed light on the underlying pathological mechanisms of follicular development in patients with PCOS. Follicular fluid samples were collected from patients with PCOS and healthy individuals. The Cell Counting Kit-8 and bromodeoxyuridine assays were performed to assess KGN cell proliferation. The expression of miR-145-5p was significantly decreased in PCOS granulosa cells than in control cells, whereas the expression of SET was increased. Furthermore, miR-145-5p suppressed the proliferation of KGN cells. SET was identified as a direct target of miR-145-5p. Additionally, SET promoted the proliferation of KGN cells, and knockdown of SET counteracted the effect of the miR-145-5p inhibitor. Therefore, miR-145-5p regulates granulosa cell proliferation by targeting the SET in KGN cells; this process may be a potential pathological pathway that contributes to follicular developmental disorders in PCOS.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"372-378"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Change in the ability of bovine granulosa cells to elongate transzonal projections and their transcriptome changes during follicle development. 卵泡发育过程中牛颗粒细胞伸长横区突起的能力变化及其转录组变化。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-10-14 DOI: 10.1262/jrd.2024-016
Mihoko Fushii, Hirohisa Kyogoku, Jibak Lee, Takashi Miyano

Granulosa cells (GCs) in secondary follicles differentiate into cumulus cells (CCs) and mural granulosa cells (MGCs) in the antral follicle. Only CCs maintain direct connections with oocytes through transzonal projections (TZPs) and support oocyte growth. Here, we examined whether granulosa cells (GCs) from secondary follicles and MGCs from early and late antral follicles were able to reconstruct complexes with TZP-free denuded oocytes (DOs) and regenerate TZPs. Furthermore, to confirm that the regenerated TZPs were functional, the development of the reconstructed complexes and oocyte growth in the complexes were evaluated. After coculture, GCs and MGCs from early antral follicles reconstructed the complexes with DOs and regenerated TZPs. Furthermore, the oocytes in the integrally reconstructed complexes grew fully and acquired meiotic competence, suggesting that the regenerated TZPs were functional. In contrast, MGCs from the late antral follicles lost their ability to elongate TZPs. As the ability to regenerate TZPs differed among cells, we analyzed the transcriptomes of GCs, CCs, and MGCs collected from follicles of different sizes. The characteristics of TZP generation coincided with the transcriptome changes in two directions: from GCs to CCs and MGCs. In conclusion, until the early antral follicle stage, bovine GCs, CCs, and MGCs have common characteristics to elongate TZPs and form antrum-like structures that support oocyte growth in vitro. Furthermore, as the follicle develops, MGCs lose the ability to elongate TZPs.

次级卵泡中的颗粒细胞(GCs)在前卵泡中分化为积层细胞(CCs)和壁层颗粒细胞(MGCs)。只有CC通过横带突起(TZPs)与卵母细胞保持直接连接,并支持卵母细胞的生长。在这里,我们研究了来自次级卵泡的颗粒细胞(GCs)和来自早期和晚期前卵泡的MGCs是否能与不含TZP的变性卵母细胞(DOs)重建复合体并再生TZPs。此外,为了证实再生的 TZPs 具有功能性,还对重建复合体的发育和复合体中卵母细胞的生长进行了评估。经过共培养后,来自早期前卵泡的 GCs 和 MGCs 与 DOs 和再生 TZPs 重建了复合体。此外,整合重建的复合体中的卵母细胞完全生长并获得了减数分裂能力,这表明再生的TZPs是有功能的。相比之下,来自晚期前卵泡的 MGCs 则失去了伸长 TZPs 的能力。由于不同细胞再生TZPs的能力不同,我们分析了从不同大小卵泡中收集的GCs、CCs和MGCs的转录组。TZP生成的特征与转录组在两个方向上的变化相吻合:从GCs到CCs和MGCs。总之,直到早期前卵泡阶段,牛的 GCs、CCs 和 MGCs 都有一个共同的特点,即拉长 TZPs 并形成支持体外卵母细胞生长的前膜样结构。此外,随着卵泡的发育,MGCs 失去了拉长 TZPs 的能力。
{"title":"Change in the ability of bovine granulosa cells to elongate transzonal projections and their transcriptome changes during follicle development.","authors":"Mihoko Fushii, Hirohisa Kyogoku, Jibak Lee, Takashi Miyano","doi":"10.1262/jrd.2024-016","DOIUrl":"10.1262/jrd.2024-016","url":null,"abstract":"<p><p>Granulosa cells (GCs) in secondary follicles differentiate into cumulus cells (CCs) and mural granulosa cells (MGCs) in the antral follicle. Only CCs maintain direct connections with oocytes through transzonal projections (TZPs) and support oocyte growth. Here, we examined whether granulosa cells (GCs) from secondary follicles and MGCs from early and late antral follicles were able to reconstruct complexes with TZP-free denuded oocytes (DOs) and regenerate TZPs. Furthermore, to confirm that the regenerated TZPs were functional, the development of the reconstructed complexes and oocyte growth in the complexes were evaluated. After coculture, GCs and MGCs from early antral follicles reconstructed the complexes with DOs and regenerated TZPs. Furthermore, the oocytes in the integrally reconstructed complexes grew fully and acquired meiotic competence, suggesting that the regenerated TZPs were functional. In contrast, MGCs from the late antral follicles lost their ability to elongate TZPs. As the ability to regenerate TZPs differed among cells, we analyzed the transcriptomes of GCs, CCs, and MGCs collected from follicles of different sizes. The characteristics of TZP generation coincided with the transcriptome changes in two directions: from GCs to CCs and MGCs. In conclusion, until the early antral follicle stage, bovine GCs, CCs, and MGCs have common characteristics to elongate TZPs and form antrum-like structures that support oocyte growth in vitro. Furthermore, as the follicle develops, MGCs lose the ability to elongate TZPs.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"362-371"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ccdc152 is not necessary for male fertility, but contributes to maintaining sperm morphology. Ccdc152 不是男性生育所必需的,但有助于维持精子的形态。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-10-26 DOI: 10.1262/jrd.2024-058
Ryua Harima, Takahiro Sasaki, Takayuki Kaneko, Fuka Aso, Hayato Takashima, Takashi Toyama, Kenshiro Hara, Kentaro Tanemura, Yoshiro Saito

Selenoprotein P (SeP) is synthesized in the liver and plays a vital role in maintaining selenium homeostasis via transport throughout the body. Previous studies have shown that SeP-deficient mice have severely reduced expression of selenoproteins essential for testicular function, leading to male infertility. We previously reported that the high expression of Ccdc152 in hepatocytes acts as a lncRNA, suppressing SeP expression in the liver. Ccdc152 reduces SeP translation by binding to SeP mRNA and decreasing its interaction with SECIS-binding protein 2. Although Ccdc152 is highly expressed in testes, its function remains unclear. Therefore, this study aimed to elucidate the role of Ccdc152 in the testes. Using the CRISPR/Cas9 system, we generated mice lacking all exons of Ccdc152 and found that SeP expression levels in the liver and plasma, as well as overall selenium homeostasis, remained unchanged. No significant differences were observed in the expression of glutathione peroxidase 1/4 or level of selenium in the testes. Subsequent investigation of the impact on male reproductive function revealed no abnormalities in sperm motility or Mendelian ratios of the offspring. However, a slight decrease in testicular weight and an increased rate of sperm malformations in the epididymis were observed. RNA-seq and pathway analyses identified the reduced expression of multiple genes related to kinesin and reproductive pathways. Based on these findings, Ccdc152 may not be essential for male reproductive function, but it may enhance reproductive capabilities by maintaining the expression of genes necessary for reproduction.

硒蛋白 P(SeP)在肝脏中合成,通过在全身的转运在维持硒平衡方面发挥着重要作用。先前的研究表明,SeP 缺乏的小鼠睾丸功能所必需的硒蛋白表达严重减少,导致男性不育。我们以前曾报道,肝细胞中高表达的 Ccdc152 可作为一种 lncRNA,抑制 SeP 在肝脏中的表达。虽然 Ccdc152 在睾丸中高表达,但其功能仍不清楚。因此,本研究旨在阐明Ccdc152在睾丸中的作用。我们利用 CRISPR/Cas9 系统生成了缺乏 Ccdc152 所有外显子的小鼠,结果发现肝脏和血浆中 SeP 的表达水平以及整体硒平衡保持不变。在谷胱甘肽过氧化物酶1/4的表达或睾丸中的硒水平方面没有观察到明显差异。随后对男性生殖功能影响的调查显示,后代的精子活力或孟德尔比率没有异常。不过,观察到睾丸重量略有下降,附睾中精子畸形率增加。RNA-seq和通路分析发现,与驱动蛋白和生殖通路相关的多个基因表达减少。基于这些发现,Ccdc152 可能不是雄性生殖功能所必需的,但它可能通过维持生殖所必需基因的表达来提高生殖能力。
{"title":"<i>Ccdc152</i> is not necessary for male fertility, but contributes to maintaining sperm morphology.","authors":"Ryua Harima, Takahiro Sasaki, Takayuki Kaneko, Fuka Aso, Hayato Takashima, Takashi Toyama, Kenshiro Hara, Kentaro Tanemura, Yoshiro Saito","doi":"10.1262/jrd.2024-058","DOIUrl":"10.1262/jrd.2024-058","url":null,"abstract":"<p><p>Selenoprotein P (SeP) is synthesized in the liver and plays a vital role in maintaining selenium homeostasis via transport throughout the body. Previous studies have shown that SeP-deficient mice have severely reduced expression of selenoproteins essential for testicular function, leading to male infertility. We previously reported that the high expression of Ccdc152 in hepatocytes acts as a lncRNA, suppressing SeP expression in the liver. Ccdc152 reduces SeP translation by binding to SeP mRNA and decreasing its interaction with SECIS-binding protein 2. Although Ccdc152 is highly expressed in testes, its function remains unclear. Therefore, this study aimed to elucidate the role of Ccdc152 in the testes. Using the CRISPR/Cas9 system, we generated mice lacking all exons of Ccdc152 and found that SeP expression levels in the liver and plasma, as well as overall selenium homeostasis, remained unchanged. No significant differences were observed in the expression of glutathione peroxidase 1/4 or level of selenium in the testes. Subsequent investigation of the impact on male reproductive function revealed no abnormalities in sperm motility or Mendelian ratios of the offspring. However, a slight decrease in testicular weight and an increased rate of sperm malformations in the epididymis were observed. RNA-seq and pathway analyses identified the reduced expression of multiple genes related to kinesin and reproductive pathways. Based on these findings, Ccdc152 may not be essential for male reproductive function, but it may enhance reproductive capabilities by maintaining the expression of genes necessary for reproduction.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"396-404"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preovulatory follicular dynamics and ovulatory events following the use of GnRH 84 h after medroxyprogesterone acetate sponge removal in postpartum buffaloes. 产后水牛移除醋酸甲羟孕酮海绵84小时后使用GnRH的排卵前卵泡动态和排卵事件。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-08-24 DOI: 10.1262/jrd.2024-040
Muhammad Usman Mehmood, Ghazanfar Ali Chishti, Muhammad Waseem, Burhan E Azam, Zahid Naseer, Muhammad Saadullah, Kehuan Lu, Yangqing Lu

Herein, we evaluated the effects of gonadotropin hormone-releasing hormone (GnRH) administration 84 h after medroxyprogesterone acetate (MAP) sponge removal on follicular growth, ovulation timing, and pregnancy per artificial insemination (AI) in cosynchronized postpartum Nili Ravi buffaloes. In this study, 58 Nili Ravi postpartum buffaloes (DIM = 103 ± 1.64) were randomly divided into two treatment groups (n = 29/treatment): GnRH-TAI-84 and TAI-84. All buffaloes were administered a MAP sponge for seven days. Upon MAP sponge removal, all the subjects received prostaglandin F (PGF) and Timed AI (TAI) was performed 84 h after sponge removal. In the GnRH-TAI-84 group, the buffaloes received GnRH alongside insemination, whereas in the TAI-84 group, the buffaloes were inseminated without GnRH administration. Follicle diameter and blood estradiol levels were measured every 6 h from 72-108 h after MAP sponge removal. The animals were checked for pregnancy using ultrasonography 40 days after AI. Animals subjected to the GnRH-TAI-84 protocol had a higher follicular growth rate and preovulatory follicle size than those in the TAI-84 group. The follicular diameter was also larger in animals that received GnRH-TAI-84 than in those that received TAI-84 90 and 96 h after MAP sponge removal. Buffaloes in the GnRH-TAI-84 group had lower estradiol concentrations at 90, 96, 102, and 108 h than those in the TAI-84 group. Ovulation in GnRH-TAI-84 buffaloes occurred 11 h earlier than that in buffaloes from the TAI-84 group. A shorter interval between AI and ovulation in GnRH-TAI-84 buffaloes (14 h vs. 25 h) led to greater pregnancies per AI (62% vs. 17%) compared to buffaloes from the TAI-84 group.

在此,我们评估了在移除醋酸甲羟孕酮(MAP)海绵84小时后注射促性腺激素释放激素(GnRH)对同步产后Nili Ravi水牛卵泡生长、排卵时间和人工授精(AI)妊娠的影响。在这项研究中,58 头 Nili Ravi 产后水牛(DIM = 103 ± 1.64)被随机分为两个处理组(n = 29/处理组):GnRH-TAI-84组和TAI-84组。所有水牛都被注射了 MAP 海绵,为期七天。移除 MAP 海绵后,所有受试者均接受前列腺素 F2α (PGF2α),并在移除海绵 84 小时后进行定时人工授精 (TAI)。在GnRH-TAI-84组中,水牛在接受人工授精的同时还接受了GnRH,而在TAI-84组中,水牛在未注射GnRH的情况下接受了人工授精。在移除 MAP 海绵后的 72-108 小时内,每隔 6 小时测量一次卵泡直径和血液中的雌二醇水平。人工授精后 40 天,用超声波检查动物是否怀孕。采用GnRH-TAI-84方案的动物的卵泡生长率和排卵前卵泡大小均高于TAI-84组。在移除MAP海绵90和96小时后,接受GnRH-TAI-84治疗的水牛的卵泡直径也比接受TAI-84治疗的水牛大。GnRH-TAI-84组水牛在90、96、102和108小时的雌二醇浓度低于TAI-84组。GnRH-TAI-84组水牛的排卵时间比TAI-84组水牛早11小时。与TAI-84组的水牛相比,GnRH-TAI-84组水牛人工授精与排卵之间的间隔时间更短(14小时对25小时),因此每次人工授精的妊娠率更高(62%对17%)。
{"title":"Preovulatory follicular dynamics and ovulatory events following the use of GnRH 84 h after medroxyprogesterone acetate sponge removal in postpartum buffaloes.","authors":"Muhammad Usman Mehmood, Ghazanfar Ali Chishti, Muhammad Waseem, Burhan E Azam, Zahid Naseer, Muhammad Saadullah, Kehuan Lu, Yangqing Lu","doi":"10.1262/jrd.2024-040","DOIUrl":"10.1262/jrd.2024-040","url":null,"abstract":"<p><p>Herein, we evaluated the effects of gonadotropin hormone-releasing hormone (GnRH) administration 84 h after medroxyprogesterone acetate (MAP) sponge removal on follicular growth, ovulation timing, and pregnancy per artificial insemination (AI) in cosynchronized postpartum Nili Ravi buffaloes. In this study, 58 Nili Ravi postpartum buffaloes (DIM = 103 ± 1.64) were randomly divided into two treatment groups (n = 29/treatment): GnRH-TAI-84 and TAI-84. All buffaloes were administered a MAP sponge for seven days. Upon MAP sponge removal, all the subjects received prostaglandin F<inf>2α</inf> (PGF<inf>2α</inf>) and Timed AI (TAI) was performed 84 h after sponge removal. In the GnRH-TAI-84 group, the buffaloes received GnRH alongside insemination, whereas in the TAI-84 group, the buffaloes were inseminated without GnRH administration. Follicle diameter and blood estradiol levels were measured every 6 h from 72-108 h after MAP sponge removal. The animals were checked for pregnancy using ultrasonography 40 days after AI. Animals subjected to the GnRH-TAI-84 protocol had a higher follicular growth rate and preovulatory follicle size than those in the TAI-84 group. The follicular diameter was also larger in animals that received GnRH-TAI-84 than in those that received TAI-84 90 and 96 h after MAP sponge removal. Buffaloes in the GnRH-TAI-84 group had lower estradiol concentrations at 90, 96, 102, and 108 h than those in the TAI-84 group. Ovulation in GnRH-TAI-84 buffaloes occurred 11 h earlier than that in buffaloes from the TAI-84 group. A shorter interval between AI and ovulation in GnRH-TAI-84 buffaloes (14 h vs. 25 h) led to greater pregnancies per AI (62% vs. 17%) compared to buffaloes from the TAI-84 group.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"349-355"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum concentrations of anti-Müllerian hormone modulate ovarian response to different doses of follicle-stimulating hormone in Japanese Black donors. 抗缪勒氏管激素的血清浓度可调节日本黑人捐献者卵巢对不同剂量卵泡刺激素的反应。
IF 1.9 4区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-12-13 Epub Date: 2024-11-10 DOI: 10.1262/jrd.2024-067
Hiroaki Okawa, Norihiro Yukiyama, Oky Setyo Widodo, Kanae Niimi, Yuta Chuman, Yasuo Fushimi, Mitsuhiro Takagi

Anti-Müllerian hormone (AMH) is a marker for predicting embryo production in cows subjected to superovulation; however, it remains to be established as a reliable predictor of reproductive performance. We hypothesized that the serum AMH concentration of donors affects the ovarian response to follicle-stimulating hormone (FSH) treatment during superovulation. Herein, we retrospectively investigated the association between different FSH doses and AMH concentrations in donor Japanese Black cows in a superovulation program and analyzed the number of total and transferable embryos recovered. The number of transferable embryos recovered from donors with high AMH levels was significantly higher than that recovered from donors with low AMH levels. Additionally, it increased further with a reduction in the FSH dose. These results illustrate that the AMH concentration is a useful marker for predicting embryo production after superovulation, and donors with high AMH levels produce more transferable embryos at low FSH doses than at high doses.

抗缪勒氏管激素(AMH)是预测超排卵奶牛胚胎生产的一个指标,但它作为繁殖性能的可靠预测指标仍有待确定。我们假设供体的血清 AMH 浓度会影响超级排卵期间卵巢对促卵泡激素(FSH)治疗的反应。在此,我们回顾性地研究了超级排卵计划中不同FSH剂量与供体日本黑牛AMH浓度之间的关系,并分析了回收的总胚胎数和可移植胚胎数。AMH水平高的供体回收的可移植胚胎数量明显高于AMH水平低的供体。此外,随着 FSH 剂量的减少,可移植胚胎数也进一步增加。这些结果说明,AMH 浓度是预测超排卵后胚胎产量的有效指标,AMH 水平高的供体在低 FSH 剂量下比高剂量下产生的可移植胚胎更多。
{"title":"Serum concentrations of anti-Müllerian hormone modulate ovarian response to different doses of follicle-stimulating hormone in Japanese Black donors.","authors":"Hiroaki Okawa, Norihiro Yukiyama, Oky Setyo Widodo, Kanae Niimi, Yuta Chuman, Yasuo Fushimi, Mitsuhiro Takagi","doi":"10.1262/jrd.2024-067","DOIUrl":"10.1262/jrd.2024-067","url":null,"abstract":"<p><p>Anti-Müllerian hormone (AMH) is a marker for predicting embryo production in cows subjected to superovulation; however, it remains to be established as a reliable predictor of reproductive performance. We hypothesized that the serum AMH concentration of donors affects the ovarian response to follicle-stimulating hormone (FSH) treatment during superovulation. Herein, we retrospectively investigated the association between different FSH doses and AMH concentrations in donor Japanese Black cows in a superovulation program and analyzed the number of total and transferable embryos recovered. The number of transferable embryos recovered from donors with high AMH levels was significantly higher than that recovered from donors with low AMH levels. Additionally, it increased further with a reduction in the FSH dose. These results illustrate that the AMH concentration is a useful marker for predicting embryo production after superovulation, and donors with high AMH levels produce more transferable embryos at low FSH doses than at high doses.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"418-422"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Reproduction and Development
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1